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    Bile Notes

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    Richel Ameryl Dasoc
    The Lieberman-Buchard test is a chemical test used to detect cholesterol. It involves mixing cholesterol crystals with acetic anhydride and concentrated sulfuric acid. A color change from blue to green or violet indicates the presence of cholesterol.

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    Bile Notes

    Uploaded by

    Richel Ameryl Dasoc
    19 views1 page
    The Lieberman-Buchard test is a chemical test used to detect cholesterol. It involves mixing cholesterol crystals with acetic anhydride and concentrated sulfuric acid. A color change from blue to green or violet indicates the presence of cholesterol.

    Original Description:

    Original Title

    BILE NOTES (1)

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    The Lieberman-Buchard test is a chemical test used to detect cholesterol. It involves mixing cholesterol crystals with acetic anhydride and concentrated sulfuric acid. A color change from blue to green or violet indicates the presence of cholesterol.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    19 views1 page

    Bile Notes

    Uploaded by

    Richel Ameryl Dasoc
    The Lieberman-Buchard test is a chemical test used to detect cholesterol. It involves mixing cholesterol crystals with acetic anhydride and concentrated sulfuric acid. A color change from blue to green or violet indicates the presence of cholesterol.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Lieberman-Buchard test:

    Cholesterol crystals + 10 drops of acetic anhydride + 2 drops of


    Conc HzSO+ -------------) mix , allow to stand , observe

    Questions:
    1. What is bile?
    Bile is a secretion of the liver. lt is viscid and has an alkaline reaction and its color is
    yellowish/greenish brown. Where is it produced in the body?
    Bile is a fluid that is made and released by the liver and stored in the gallbladder.
    2. What is the role of bile in digestion?
    It breaks down fats into fatty acids, which can be taken into the body by the digestive tract.
    It also aids in the elimination of waste products from the body.
    3. What are the components of bile?
    Its important constituents are bile acids, bile pigments, inorganic salts and cholesterol.
    4. What are Bile salts? Bile Pigments?
    Bile salts are polarized steroids that play a vital role in intestinal fat absorption as primary
    components of bile. While bile pigments are highly colored waste products of the
    hemoglobin of old red blood cells, found in the bile which functions as coloring matters (as
    bilirubin or biliverdin) in bile.
    5. What are the following substances:
    1. Urobilinogen is a colorless by-product of bilirubin reduction. It is formed in the
    intestines by bacterial action on bilirubin.
    2. urobilin or urochrome is the chemical primarily responsible for the yellow color of
    urine.
    3. Bilirubin is a yellowish substance in your blood. lt forms after red blood cells break
    down.
    4. Stercobilin is the chemical responsible for the brown color of human feces and is
    one end-product of heme catabolism.

    6. What is emulsification? Discuss the roles of the bile acids and bile salts in this process.
    Emulsification is a process in which large lipid globules are broken down into several small
    lipid globules. Through the process of enrulsification, bile acids break down large lipid droplets
    into smaller ones, increasing the surface area for digestive enzymes. Emulsification is possible
    due to the amphipathic property of bile salts that digests lipids into fatty acids and monoglycerides.
    7. What does excessive amount of bile indicate?
    It indicates bile acid malabsorption (BAM), a condition that occurs when your intestines
    can't absorb bile acids properly. This results in extra bile acids in your intestines, which can cause
    watery diarrhea.

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