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TABLE OF CONTENTS
UNIT 1: CLASSIFICATION OF KINGDOM ANIMALIA ................................................................................. 1
1.1. Introduction................................................................................................................................................. 1
3.2 Osmosis............................................................................................................................................................24
4.1 Definition........................................................................................................................................................30
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5.1 Introduction...............................................................................................................................................35
UNIT 7: PHOTOSYNTHESIS................................................................................................................................45
7.1 Introduction...............................................................................................................................................45
7.2 Necessity for chlorophyll, light energy and carbon dioxide for photosynthesis ..............45
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12.4 Symptoms, prevention, control and treatment of common infectious diseases .............88
13.1 Introduction..............................................................................................................................................94
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15.5 Abortion....................................................................................................................................................116
Glossary ....................................................................................................................................................................122
References................................................................................................................................................................126
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Dear learner,
You are already in senior four of A’ level with Biology as a core subject, and you became familiar with the
subject of Biology. Studying Biology is not difficult, you just need certain good strategies to be successful:
1. Read your syllabus before starting the lesson in order to prepare the subject content of the day.
Write down notes of important subject contents and questions raised.
2. During the course delivery, please do follow carefully teacher’s comments and highlight main
points.
3. Revise daily your notes and record encountered difficulties.
4. Make research and do as many exercises as possible to fix properly the learned content.
5. Do your home works. Your homework should be done as soon as possible. Do not wait till the
day of submission of the home works. Do not let other people work for you. Do the homework
yourself, it is for your benefit.
6. “Good luck” in science means to work hard.
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a. Class Pisces
The term ‘Pisces’ is derived from a Latin word ‘Piscis’ which means fish. Therefore, the class pisces is made
up of the fish family. Ee.g.: Tilapia, Shark and Mud fish
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4. Fish have fins that aid in movement. The fins on the sides of the body are usually found in pairs, for example,
the pelvic and pectoral fins. The dorsal, ventral and tail fins are usually unpaired.
5. Fish are poikilothermic: Their body temperature is dependent on environmental temperature because they
cannot regulate their own body temperature.
6. Fish exhibit external fertilisation where eggs are first laid by the female then the male sheds sperms over
them.
7. Fish have a lateral line on their body for sensitivity.
8. Fish have a single circulatory system with a two chambered heart.
Note: Fish use gills for gaseous exchange except lung fish that lives in oxygen deficient swamps and use
lungs for breathing.
b. Class Amphibia
The word amphibia comes from the word ‘amphi’ which means ‘dual’ or ‘two’. This class of chordates can
live both on land and in water. Most adult amphibians live on land. However, they go back to the water to
breed. Examples are: Frog, Toad, Salamander, Newt.
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c. Class Reptilia
The term ‘Reptilia’ comes from a latin word, ‘Reptilis’ which means ‘crawl’. The animals in this class move
by creeping or crawling. Unlike amphibians, reptiles do not breed in water because they lay eggs with
leathery shells; hence cannot dry out. Examples of reptilia are; snakes, turtles, tortoises, crocodiles and
lizards.
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5. Birds have different types of feet to adapt them to their different environments. These include:
• Feet for grasping – like those of a kingfisher. They are large and curved to grasp a prey tightly.
• Feet for scratching – like those of chickens. They have nail-like toes to scratch the soil for food.
• Swimming birds – like ducks have webbed feet used like paddles.
• Perching feet – like that of a robin. They have long back toes to grab and perch tightly on a tree branch or
bark.
• Feet for running – like those of ostrich. They have three toes to enable stability when running.
e. Class Mammalia
The term Mammalia is derived from the Latin word ‘mammalis’ which means ‘mammal’ or ‘mamma’ which
means milk secreting organ of female mammals. Most mammals are terrestrial except a few like dolphins and
whales which are aquatic.
Exapmles of mammals: dogs, camel, humman being,
Main characteristics of animals in Class Mammalia
1. They have mammary glands. They suckle and take care of their young ones.
2. Their bodies are covered with fur, hair or wool.
3. They give birth to young ones except the duck-billed platypus and spiny ant-eater which lay eggs.
4. They have external ears. These are the only class of chordates that have the external ears.
5. They exhibit internal fertilisation.
6. They are Homeothermic. Their body temperatures do not depend on the environment. It is maintained
constantly.
7. They have differentiated teeth (i.e incisors, canines, pre-molars and molars) each with different function.
They are therefore referred to as heterodonts. Others with uniform teeth differing only in size are
homodonts.
3. They have bilateral symmetry. This means that they can be cut into two similar halves in only one way.
4. Muscles for movement are attached on the cuticle or exoskeleton, for example in insects.
5. They have a fluid filled body cavity called haemocoel for example in earthworms.
6. Their bodies are segmented.
Organisms in phylum Arthropoda can further be grouped into various classes. The organisms are grouped on
the basis of:
• Number of legs
• Presence and absence of antennae
• Number of antennae
• Number of body parts
• Type of eyes
The 5 classes of phylum Arthropoda are: Insecta, Arachnida, Crustacea, Diplopoda and Chilopoda
a. Class Insecta
This is the largest class in the phylum Arthropoda. The term ‘insecta’ comes from the word ‘incised’ which
means ‘cut.’ The body of organisms in this class are divided into three distinct parts; that is head, thorax
and abdomen.
Main characteristics of organisms in class Insecta
1. They have three distinctive body parts; head, thorax and abdomen.
2. They have a pair of long antennae.
3. They have three pairs of jointed legs, which are attached to their thorax.
4. They have a pair of large compound eyes.
5. Some have one or two pairs of wings that are attached to the thorax.
6. They breathe by means of spiracles, which are found on the sides of the abdomen and thorax.
b. Class Arachnida
This class of arthropods includes spiders, mites, ticks and scorpions.
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c. Class Crustacea
The term crustacea is derived from “crusta” which means a ‘hard shiny coat’. They are aquatic arthropods
except for wood lice which are the only fully terrestrial crustaceans.
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d. Class Diplopoda
Diplopoda means arthropods with two pairs of legs per segment. They also have elongated cylindrical
bodies. Diplopods are herbivores and mainly feed on vegetables and dead organic matter. This class consists
of millipedes. Millipedes are of various sizes. There are giant millipedes and small sized millipedes.
Main characteristics of organisms in class Diplopoda
1. They have elongated and cylindrical bodies.
2. Their bodies are divided into two main parts; the head and a segmented trunk. The number of body segments
ranges from 25 – 100.
3. Each body segment has two pairs of legs except the first thoracic segments that have one pair of leg each.
4. They have a pair of antennae.
5. They may have simple or compound eyes, or in some cases no eyes are present.
6. They breathe through spiracles found on the sides of the body segments.
7. Millipedes roll their bodies when disturbed. After coiling, they produce a stinking substance from their
stink glands. This is self- protective behaviour against enemies and predators.
e. Class Chilopoda
This class is composed of centipedes. They are found in virtually all habitats but unlike the millipedes, they
have less number of legs. Centipedes have only one pair of legs per segment.
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W X Y Z
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a) To which phylum do the above organisms belong? Give reasons for your answer.
b) How does animal Z protect itself from enemies?
c) Which animal above belong to a class that lacks antennae? Name the class.
11. Some animals under class Arachnida are dangerous. They can both bite and sting. Suggest ways in which
such organisms can be handled.
12. Discussion on the topic "are insects important or not important to our environment?"
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(a) Light - The sun is the main source of light energy in all ecosystems on earth. Light is important in any
ecosystem because plants use it to make their own food.
(b) Temperature – Temperature variation in an ecosystem affects the type and distribution of organisms
found in it. Some organisms prefer high temperatures while others prefer low temperatures. Therefore
they will inhabit different parts of the ecosystem.
(c) Atmospheric pressure – This is the pressure exerted by air in the atmosphere. Atmospheric pressure
affects the amount of oxygen in the air. At the sea level, air pressure is high. This reduces at higher
altitudes. Therefore different organisms will occupy different altitudes based on their endurance.
(d) Salinity – This is the degree of salt concentration in water. This is a factor that is mainly found in aquatic
habitats. Some organisms live in salty environments while others live in fresh water environments.
(e) Humidity – This is the amount of water vapour in the atmosphere. It influences evaporation and
transpiration rates.
(f) pH– This is the degree of acidity or alkalinity. The pH of an ecosystem affects the type of distribution of
organisms found in it.
(g) Wind – This is moving air. Air moves in different directions and speed. Wind influences environmental
factors such as temperature and humidity.
ii. Biotic: Anything that is, or has ever been, alive. Examples of biotic factors in an environment include
organisms, organic molecules, and cells. Biotic is the opposite of abiotic.
iii. Carrying capacity:The maximum number of individuals in a single population that a given
environment can sustain at a given time.
iv. Community: A group of two or more populations of organisms from different species inhabiting the
same location at the same time.
v. Decomposer: An organism that feeds on and breaks down dead or decaying matter in the process
of ecological decomposition. Examples of decomposers include fungi, like mushrooms and molds; worms,
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like earthworms and some nematodes; and some bacteria. Decomposers are also called saprotrophs,
meaning "putrid eaters."
vi. Ecosystem: A term describing all the living and nonliving things in a certain location. Ecosystem studies
in ecology explore the interactions between organisms, like individuals, populations, or communities, and
the abiotic components in the environment, like chemicals, landscapes, and the like.
vii. Food chain: A simple, direct, and trophic, or eating, relationship among a group of organisms, where
one organism, like a plant, is the food source for the next organism, like a cow, which in turn is the food
source for the next organism, like a human, and so on and so forth.
viii. Food web: A complex trophic relationship among a group of organisms, consisting of interactions
among multiple food chains (see definition above). A food web describes how
multiple producers and consumers directly or indirectly interact in an ecosystem.
ix. Habitat: The physical environment where a population of a single species lives, or inhabits. A habitat
consists of all the abiotic, or nonliving, resources influencing the population.
x. Niche: An organism's role in an environment, including how it uses its resources, relates to other
organisms, and times its reproduction. Each individual organism has a niche in its population, community,
and ecosystem, but niches are flexible and change depending on circumstances.
xi. Population: A group of organisms of the same species living in the same geographic area.
xii. Biosphere: This is the part of the Earth and its atmosphere capable of supporting life. It is an area where
organisms live, including the ground and the air.
xiii. Producer: This refers to all green plants (for example, beans, mango tree and pine) which manufacture
their own food by the process of photosynthesis.
xiv. Biome: This is a large naturally occurring community of animals (fauna) and plants (flora) occupying a
major habitat. Examples include: forest, grassland, freshwater, marine and desert.
xv. Biomass: This is the total mass of organisms in a given area or volume.
xvi. Edaphic factors: These are all non-living components of an ecosystem both chemical and physical.
They are related to abiotic factors.
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A food chain is a linear representation of how organisms eat each other before they are eaten in return. It is a
sequence describing a feeding relationship between producers and consumers.
Examples offood chains
A food web is a complex series of interconnected food chains. It shows feeding relationships between
various species of organisms in a given community. A food web is composed of all possible food chains in
any given ecosystem. When a schematic representation is made to show other options an organism has for
food, a food web is obtained.
Examples of food webs
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e.g.: 1
e.g.:2
The food web comprises the relationship within a community. It is easily made by arranging organisms in
their trophic levels.
The arrows in the food chain indicate the flow of energy from the food web. One organism can provide
energy to more than one trophic level.
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Figure 2.4: energy flow in an ecosystem illustrating how energy is lost from one feeding level to the next
Example: in the first trophic level in a terrestrial environment, the amount of energy produced by the
autotrophs is 10000kcal. The amount of energy at the primary consumers is 1000kcal and that at secondary
consumers is 100kcal. Calculate amount of energy transferred from producers to primary consumers.
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1000
TLTE = x 100
10000
TLTE = 10%
2.6 Ecological pyramids
Ecological pyramids are diagrams that show how important factors in an ecosystem such as energy, biomass
and population size change at each trophic level. Traditionally, these diagrams place the primary producers
(green plants) at the bottom. The highest trophic levels are placed at the top. The size of the portion of the
diagram associated with each trophic level shows the amount of factor in consideration. A food chain can be
expressed in a measurable way by using pyramid of numbers or pyramid of biomass.
2.6.1 Pyramid of numbers
The pyramid of numbers is a diagramatic representation of numbers of organisms in each trophic level in a
food chain. In this way, it is possible to know the number of organisms that are capable of transferring
energy from one trophic level to the next.
Sometimes the pyramid is not upright, for instance, if you were to construct pyramid of numbers using the
tree as a habitat. It would be the only producer and the consumers such as caterpillars and birds would be
many. If we were to construct pyramid of numbers, the smallest box would represent the tree. It would be at
the bottom and not at the top. The shape of this pyramid would be inverted as can be seen in the figure
below.
Figure 2.5: Pyramids of numbers (a) glassland (b) parasitic food chain
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a. Name the living things in the food web that are producers.
b. Name the living things in the food web that are consumers.
c. Which living things does the snake eat?
d. Which living things does the hawk eat?
e. What is eaten by the rabbit?
3. During an ecological tour of Lake Muhazi, a group of students from Kagarama Secondary school recorded
the following observations.
(i) Tilapia feeds on mosquitoes larvae.
(ii) Mosquito larvae feed on planktonic algae.
(iii) Planktonic crustaceans feed on planktonic algae.
(iv) Hawks feed on tilapia, worms and planktonic crustaceans.
(a) From this record of observations, construct a food web.
(b) From the food web you have constructed in (a) above, isolate and write down a food chain that ends with
(i) Hawk as a secondary consumer
(ii) Hawk as a tertiary consumer
(c) (i) Which group of organisms in this lake are the producers?
(ii) The biomass of the producers in this lake was found to be greater than that of the primary
consumers. Give an explanation for this observation.
(d) Using the food web you constructed in (a) above, name
(i) Two organisms that compete for food in the lake?
(ii) The source of food the organisms in (d) (i) above compete for.
(e) State ways by which human beings may interfere with this lake ecosystem.
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b. Concentration gradient
The greater/bigger the concentration gradient the faster the rate of diffusion.
When the concentration gradient is low, diffusion rate is also slower.
c. Size of molecules
Larger molecules are heavier and will diffuse at a slower rate compared to smaller molecules which are
lighter.
d. Diffusion distance
The shorter the distance of the membrane, the faster the rate of diffusion. It takes a longer time for molecules
to diffuse across a thick membrane while It takes less time for molecules to diffuse across a thin membrane.
e. Surface area to volume ratio
When the surface area to volume ratio is large, more of the substance diffuses across it than when it is small.
Importance of diffusion odf gases and solutes and water as a solvent
Gases
Diffusion of gases is important because:
It is necessary for the process of gaseoaus exchange in all living organisms. For example, in
humanbeings, oxygen moves from a region of high concentarion in the airsacs to a region of low
concentration in the blaood.
During the process of photosynthesis, plants are able to obtain carbon dioxide from the air and
release oxygen into tha air.
Solutes
The importance of diffusion of solutes is;
It enables absorption of diisolved nutrients in living organisms such as bacteria and fungi.
It enables dissolved salts to get into the plant through the root hair cell.
It helps in absorption of some digested foods.
Water as a solvent
Water is important as a solvent because:
Waste products in living organisms cannot be excreted unless they are dissolved in water.
Secretion of enzymes and hormones cannot take place unless they are dissolved in water
Minerals have to be dissolved in water for them to be absorbed by plants.
About 75% of water is contained in most cells.
3.2 Osmosis
Osmosis is the movement of water molecules from a region of high water potential (dilute solution) to a
region of low water potential (concentrated solution) through a partially permeable membrane.
Since the concentration of solutions is defined in terms of solute concentration and not in terms of water
content; water molecules diffuse from less concentrated solution (fewer solutes, more water) to a more
concentrated solution (more solute, less water).
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Concentration of a solution depends on the amount of solute dissolved. A dilute solution has more water
molecules compared to solute molecules whereas a concentrated solution has more solute molecules than
water molecules.
Osmosis is an important way of moving water in and out of the cell when needed. If a cell uses up water in
its chemical reactions, the cytoplasm becomes more concentrated and more water will immediately move in
by osmosis. Similarly, if the cytoplasm becomes too dilute due to water produced during chemical reactions,
water will leave the cell by osmosis, restoring the balance.
However, osmosis can cause some serious problems in animal cells. If the solution outside the cell is more
dilute than the cell content (hypotonic solution), then water will move into the cell by osmosis, diluting the
cytoplasm. The cells will swell and may eventually burst. If the solution outside the cell is more
concentrated than the cell contents (hypertonic solution), then water will move out of the cell by osmosis.
The cytoplasm will become very concentrated and the cell will shrivel up.
Osmosis in plant cells
When water moves into a plant cell by osmosis in this way, the vacuole gets bigger and the cytoplasm is
pressed against the cell walls. The force exerted on the cell walls is called turgor. When the cytoplasm is
pressing against the wall of the cell so hard that the more water can enter the cell, the cell is said to be fully
turgid. The rigid cells give the plant strength and support. They allow the stems, leaves and flowers to be
upright so the tissues can function properly.
If the surrounding becomes more concentrated than the plant cells, then water will leave the cell by osmosis.
The vacuole shrinks and the cell becomes much less rigid, it is flaccid (flabby). If water continues to leave
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the cell by osmosis, eventually the cytoplasm pulls away from the cell walls. This process is called
plasmolysis (the shrinking of protoplasm away from the cell wall of a plant due to water loss) and the cell is
plasmolysed.
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Insectivorous plants such as Pitcher plant trap insects when there is a sudden change in their turgor pressure
when disturbed by the insect.
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5. Slices of onion epidermis were placed in different concentrations of sucrose solution. The percentage of
plasmolysed cells was determined after thirty minutes. The results were as follows:
Conc. of sucrose solution (Molar) 0.55 0.6 0.65 0.7 0.75
% of plasmolysed cells 0 5 20 80 100
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(e) What is the relationship between molar concentration of sucrose solutions, and percentage of plasmolysed
cells?
(f) What description or term would be used on a plant where 100% of its cells were plasmolysed?
6. Two potatoes were peeled. One of them was boiled. A cavity was made in each of the potatoes and the
experiment was set up as shown below. The experiment was left undisturbed for 24 hours.
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The moveent of substances across a mebrane by active requires carrier proteins. They pick up
molecules of a substance from one side of the cell membrane and transport them across against the
concentration gradient. Carrier protein molecules have binding sites for molecules. the molecules to
be transported bind to the carrier protein using energy. The carrier proteins change shape and the
particles are transported to the other side of the membrane, and they difuse away from the carrier
protein.
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a. Phagocytosis
Phagocytosis is a process by which a cell engulfs large molecules to form a phagocytic vesicle enclosing the
particles. Phagocytosis is also known as “cell eating”. This is because the molecules are ingested.
Phagocytosis occurs in specialized cells called phagocytes.
2. Exocytosis
Exocytosis is a process by which the contents of a cell vacuole are released to the exterior through fusion of
the vacuole membrane with the cell membrane.
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Materials removed include solid particles, undigested remains from food vacuoles among others.
Across
2. Type of transport that requires energy.
3. Prefix that means “inside”.
4. The engulfing of large particles.
5. Prefix that means “outside”.
Down
1. Type of transport that does not require energy.
6. Active transport will remove...........ions, while taking in potassium ions.
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Based on their reaction with Benedict’s solution sugars are grouped into two.
• Reducing sugars
• Non-reducing sugars
i. Reducing sugars
Benedict’s solution is a clear blue solution used to test simple/reducing sugar, such as glucose and fructose
as well as disaccharides like maltose.
In the presence of simple sugar, the blue solution changes colour to green, yellow and brick-red (orange)
depending on the amount of sugar.
Material required: bunsen burner, waterbath, thermometer, test tubes, marker, dropper, test tube stand, test
tube claps.
Chemicals required: Benedict’s solution, distilled water.
Food sample: glucose
Procedure:
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1. Mix small amount of each food sample (glucose) with distilled water to make a test solution.
2. To a test tube, add 1cm3 of solution to be tested.
3. If testing more than one solution, label each test tube with a marker.
4. Add 10 drops of Benedict’s solution to each test tube.
5. Carefully heaat the test tubes by suspending in a hot waterbath at about 40-50 degrees celsius for five
minutes.
6. Note any colour change.
If sugar is present, solution will turn gree, yellow, orange or red-brown depending on sugar concentration.
Procedure
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The solution which was initially blue becomes yellow, from yellow to green, from green to orange or red-
brown precipitate.
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Procedure:
1. To a test tube, add food sample (egg albumen or milk) to be tested.
2. Add 3 to 5 drops of Biuret reagent solution and shake gently to mix.
3. Note any colour change.
Pink or purple colour indicates the presence of proteins.
Caution: Biuret solution is corrosive and can cause burning sensation. Handle it carefully.
Note: The Biuret’s test uses several reagents that are mixed in equal proportions. Copper (II) sulphate and
sodium hydroxide make up the reagent.
Lipids are composed of fats and oils. Fats are solid at room temperature while oils are liquid at room
temperature. Cooking oil, animal fats, groundnuts, meat among others, all contain lipids.The ethanol
emulsion test is a food test which determines the presence of lipids.
Materials required: test tubes, marker, dropper. Measuring cylinder, test tube stand.
Chemicals required: ethanol, distilled water.
Food sample: vegetable oil
Procedure:
1. Take food sample (vegetable oil) and place in a dry test tube.
2. Add 95% ethanol into the tube and shake thoroughly.
3. Pour this solution into another test tube of distilled water.
4. Observe for changes.
A white (milk-like) emulsion indicates the presence of fats or oils.
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3. Explain the chemical tests that you can carry to identify the presence of proteins in a food sample.
4. Outline the steps followed in identifying a reducing sugar in a food substance.
5. When one is testing for starch, a blue-black precipitate is usally formed, explain.
6. Name the positive results for identifying starch, proteins and vitamin C.
7. Some drops of fresh pineapple fruit juice are needed drop by drop to DCPIP solution. The deep blue
colour of the DCPIP quickly fades.
a. What does the disappearance of the blue colour indicate about the type of food substance present
in the juice?
b. What is the importance of this food substance to the human body?
8. (a) A test tube containging some sucrose solution was treated with Benedict’s solution and then
heated gently. The mixture remained blue. What does this indicate about sucrose?
(b) In another test tube, a few drops of dilute hydrochloric acid were added to some sucrose solution and the
mixture was boiled. What chemical change will take place?
(c) After cooling the mixture in (b), a few drops dilute sodium hydrogen carbonate solution were added in
the mixture. Explain this reaction.
(d) After a little while, a few drops of Benedict’s solution were added to the mixture obtained in (c) above. A
deep orange precipitate formed. What conclusion can you draw from this result about sucrose?
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UNIT 6: ENZYMES
6.1. What are enzymes?
Enzymes are biological catalysts that are proteins in nature. They speed up or slow down the rate of
chemical reactios in the body without being used up themselves. These reactions taking place in the living
organisms are referred to as metabolism and they occur in much the same way as any other chemical
reactions in a laboratory.
6.2 Characteristics of enzymes
All enzymes are proteins in nature.
Enzymes are produced in living cells.
Enzymes are specific- this means that an enzyme catalyses only one kind of substrate and not others.
Enzymes are affected by changes in temperature and pH – an enzyme works best at specific optimum
and pH.
Some enzymes work best at low pH (acidic medium) while others work best at high pH (alkaline
medium). Most enzymes in the human body for instance work best at neutral or slightly alkaline pH.
Examples are: lipases, peptidases and amylase. A few enzymes like pepsin that digests proteins in
the stomach works best at an acidic pH of 2.
Enzymes work rapidly- enzymes work very fast in converting substrates into products.
Enzymes are efficient – this means
They are required in very small amounts.
They are not used up in a reaction and can therefore be reused.
Enzymes catalyse reversible reactions: this means that enzymes can change a substrate into products
and the products back to the original substrate.
Active sites of enzymes can be deactivated or slowed down by the substances called inhibitors, e.g:
cyanide (a poison).
he temperature destroy enzymes. Such temperatures destroy the binding sites of enzymes and the enzymes
are said to be denaturated. A denatured enzyme cannot function. In human beings, most enzymes begin to
denature at 40°C.
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1. Enzymes are important in living organisms for the following reasons except.
a. They sustain life. c. They speed up the rate of reaction.
b. They slow down the rate of reaction d. They are catalysts.
2. What is the name of the part of the enzyme where the substrate binds?
a. Active transport c. Active transport
b. Substrate d. Product
3. Most enzymes are............
a. Vitamins c. Proteins
b. Carbohydrates d. Fats
4. Define catalysts.
5. State four characteristcs of enzymes.
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UNIT 7: PHOTOSYNTHESIS
7.1 Introduction
Plants are autotrophs. This means that plants can synthesize their own food. Plants use energy from the sun,
carbon dioxide and water as raw materials to make food in process known as photosynthesis. They have
chrolophyll which traps light energy from the sun. The chlorophylls are found in chloroplasts. Chloroplast
is an example of a plastid. It is an organelle in a plant cell where photosyhntesis takes place. Cells that have
chloroplasts are called photosynthetic cells. During photosynthesis, oxygen is given off as a by-product.
chlorophyll
Water + carbon dioxide > glucose + oxygen
sunlight
7.2 Necessity for chlorophyll, light energy and carbon dioxide for photosynthesis
a. Chlorophyll
Chlorophyll is a pigment that gives plants and algae their green colour. It is necessary foe photosynthesis
since it absorbs light energy required. It converts the light energy to chemical energy which synthesises
water and carbon dioxide to form glucose and oxygen. Leaves that do not have chlorophyll do not undergo
photosynthesis.
b. Light energy
Light energy is necessary for photosynthesis because it provides the energy needed to ensure the reaction
occurs.
c. Carbon dioxide
CO2 is necessary for photosynthesis because it is converted into sugars such as glucose that is needed by the
plant.
7.3 Limiting factors of photosynthesis
A factor is called a limiting factor bacause it restricts the rate at which the process can take place. Three
factors can limit the speed of photosyhntesis: amount of CO2, light intensity and temperature.
a. Carbon dioxide concentration
b. Light intensity
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The rate of bubbles given off per unit of time, from the cut end of the water plant (Hydrilla) is
measured. The results obtained are shown in the table below:
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Xylem is the system of tubes and transport cells that conducts water
and dissolved minerals. They have different types of elements:
Tracheids: long, thick, spindle shaped with pits in their thick
walls. Water flows through these pits.
Vessels: are long, cylindrical cells with a spacious lumen. Cells
are arranged end to end and cross walls are completely
dissolved. These perforations allow water to easily flow
through xylem vessels.
Functions of xylem
The main function of xylem is to conduct water and minarals from
the roots to other parts of the plants. Xylem also supports and
strengthens the systems of plants.
Function of phloem
The phloem transports food substances made during photosyntesis (sugar and amino acids) from leaves to
other parts of the plant. The movement of food substances from the leaves to other parts of the plant is
known as translocation.
8.3 Distribution of vascular tissue (xylem and phloem)
Xylem and phloem run parallel to the length of the stem and root. They occur side by side to form
continuous longitudinal channels. They are found in leaves, stems and roots.
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Figure 8.4: T.S of dicot and monocot systems showing xylem and phloem
3. Root: the vascular system is discontinuous. Xylem forms a hard central water conducting core.
8.4 Water and minerals uptake
Water uptake
Water is absorbed from the soil by roots and transported by xylem vessels to other parts of the plants. The
soil particles are usually surrounded by a film of water except during dry spells. Root hair cells absorb
water from the soil by osmosis.
The sap of the root hair cell is hypertonic to the soil particles. This is because it has dissolved sugar and
salts. Due to this concentration gradient, water molecules move by osmosis from the soil through the
epidermis of root hair cells into the cell sap.
The water moves from the epidermis through the cortex. The water is travelling between the cells. The
water then passes through the cortex. The water is travelling through the cytoplasm of the cells that are
found in the endodermis before entering the xylem. It then moves to the xylem vessels found in the stem
then to leaves. Xylem connects roots to the leaves through stem and branches. In this manner, water and
minerals are carrier to the entire plant. The water escapes through the stomata (transpiration).
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3. Soil air: for water to be absorbed by the roots, energy is needed. Energy is produced during the
process of respiration hence the need for soil air which determines the rate of respiration.
4. Transpiration rate: the rate of transpiration affects the rate of water uptake. This is because of
transpiration pull in the xylem vessels which ensures that water is pulled upwards.
Minerals uptake
Mineral uptake refers to transportation of minerals from the soil into the plant through the roots.
Xylem vessels transport minerals to other parts of the plant through the process of active transport. Roots
hair cells uptake ions from the soil actively (using energy in the form of ATP). Therefore, there is need for
abundant oxygen supply to the soil for respiration to take place.
8.5 Source and sink of food products.
A source is a structure (or an area) in the plant that produces sugars. Leaves are examples of sources. This is
where sugar is manufactured through the process of photosynthesis.
Sinks are areas in the plant where sugars are stored or used. Generally sikns are growing roots, stems,
developing leaves and fruits.
Phloem tissue transport food (sugars) from sources to sinks.
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Figure 8.7: A potometer (a device used for measuring the rate of water uptake of a leafy plant shoot. The
main reason for water uptake by a cut shoot is transpiration (evaporation in plants) and is affected by the
transpiration stream.)
8.7 Wilting in plants
Wilting is a condition in plants where more water is lost by evaporation that is absorbed from the soil.
Temporary midday wilting is common during hot, sunny afternoons but plants can rehydrate over night
when lower temperatures result in decreased water losses.
Wilting in plants makes both leaves and flowers to droop. Drooping of leaves is advantageous since it
reduces the surface area of the leaf exposed to the atmosphere thus reducing the rate of transpiration.
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b. Mesophytes
Mesophytes are plants which grow under moderate to hot and humid conditions. For example, maize,
tomato,...
Adaptations of mesophyes
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1. Asthma
This is a disease that comes about when the air passages in the lungs suddenly narrow as a result of
contraction of their smooth muscles. It is also characterised by an inflow of mucus which clogs the narrow
passages even more. Some substances can trigger an asthma attack. They are called allergens. They include
pollen grains, some type of proteins in milk, pet hairs, dust and even flavours in food. It can also be caused
by stress and anxiety. In some families the disease is inherited.
Symptoms of asthma
• Difficulty in breathing. Breathing can feel so difficult or quick that the patient can faint.
• Wheezing sounds when breathing.
Prevention and treatment
Asthma is treated by two types of medication: long-term control and quick-relief medicines that help reduce
airway inflammation and prevent asthma symptoms.
Quick-relief medicines relieve asthma symptoms that flare up.
People with asthma should avoid an environment that is likely to bring on asthmatic attack. This is
by avoiding contact with allergens.
Asthma patients are advised to carry inhalers that contain a drug which pacifies the condition.
2. Bronchitis
This is an infection of the inner walls of the bronchi. It is caused by bacteria or air pollutants such as smoke
in inhaled air. The infection causes the mucous membrane in the respiratory tract to produce excess mucus.
This causes the cells lining the bronchi and bronchioles not to function properly. As a result, the air passage
to the alveoli becomes blocked with mucus. Severe coughing occurs in an attempt to get rid of the excess
mucus. Breathing also becomes difficult. Bronchitis may be acute or chronic.
Acute bronchitis starts quickly and stops after a few days. The symptoms of acute bronchitis are like those
of a cold.
Chronic bronchitis starts slowly and lasts for a long time. It is a more serious kind of infection. It is
commonly caused by smoking and air pollution.
Symptoms of bronchitis
• Secretion of excess mucus
• Coughing
• Difficulties in breathing.
Prevention and treatment
• Acute bronchitis is treated by simple measures that include: getting plenty of rest, drinking lots of fluids
and taking a cough syrup.
• People with chronic bronchitis should take antibiotics every time they have a cold with a fever.
• A doctor should be consulted at the early stages of bronchitis.
• Avoid smoking whether directly or passively.
• Avoid polluted air.
3 . Emphysema
This results from long untreated bronchitis where the bronchioles in the lungs become blocked. This causes
damage to delicate walls of the alveoli due to high pressure when coughing. This leads to reduced surface
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area for gaseous exchange. The lungs become distended and inelastic that gases cannot be exchanged
efficiently.
The patient becomes weak due to insufficient oxygen supply to tissues.
Running and walking can prove to be hard when one has this condition.
Prevention and treatment
Emphysema is treated according to the severity of symptoms. Bronchodilators are normally given to help
relieve coughing, shortness of breath and breathing problems.
Early treatment of bronchitis with antibiotics to prevent secondary infection can help to prevent emphysema.
4. Pneumonia
Pneumonia is an infection of the lungs. It is caused by bacteria called Pneumococcus that spreads through
the air. It can also be caused by a virus or a fungi. Infection proceeds from the mouth down into the lungs.
As a result of the infection, a fluid is produced which collects in the alveoli. The lungs become solid and
have no air. This prevents exchange of gases in the lungs.
Signs and symptoms
• Sudden chills and high fever.
• Rapid shallow breathing and sometimes wheezing.
• Cough with yellow, greenish colour or mucus with some blood.
• Chest pains.
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6. Whooping cough
Whooping cough is caused by bacteria called Bordetella pertussis. The mode of infection is from one person
to another through inhalation of infected droplets.
Signs and Symptoms
• Whooping cough starts like a cold with fever, running nose and cough.
• Two weeks later, the whooping begins. The patient coughs rapidly many times without taking a breath,
until one coughs up a mass of sticky mucus, and the air rushes back into the lungs with a loud whoop
sound. After the “whoop”, the patient may vomit. Between coughing bouts the patient seems fairly
healthy.
1. Patients should consult a doctor for adequate treatment. Treatment for whooping cough involves taking
antibiotics early before coughing fits begin.
2. Patients should be isolated from contact with other people.
3. Immunisation with vaccines against whooping cough. In infants, the vaccine against whooping cough is
usually combined with those against diphtheria, tetanus and poliomyelitis.
7. Common cold
A common cold is an illness caused by a virus infection located in the nose. Colds also involve the sinuses,
ears and bronchial tubes. Colds last on average for one week. Mild colds may last only 2 or 3 days while
severe colds may last for up to 2 weeks. A cold is a milder illness than influenza.
Nasal secretions containing cold viruses contaminate the hands of people with colds as a result of nose
blowing, covering sneezes and touching the nose. Cold viruses may contaminate objects and surfaces in the
environment of a patient.
Note: Young children are prone to colds.
Cold virus, which is expelled into the air in coughs and sneezes, may land in the nose or eye of another
person and cause infection. Hence transmission.
Signs and symptoms
• Sneezing
• Runny nose
• Nasal obstruction
• Sore or scratchy throat
• Cough
• Hoarseness
• Mild general symptoms like headache, feverishness, chilliness, and not feeling well in general.
Prevention and treatment
Cold is caused by a virus, therefore it will clear after several days. Treatment is mainly tolessen the
symptoms. If a cold persists seek medical advice.
To prevent catching a cold, limit contact with known cold patients, especially during the first three days of
their illness. Practice preventive measures which keep cold virus from entering the nose:
• Wash hands after contact with cold sufferers and objects and surfaces they may have contaminated.
• Keep fingers out of the eyes and nose.
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• Avoid having cold patients cough and sneeze on you or in your direction.
8. Influenza
Influenza or 'the flu' is a highly contagious disease caused by infection from influenza type A or B (or rarely
C) virus. These viruses infect the upper airways and lungs. The flu is highly contagious.
Flu is not the same as a common cold, and can be a serious illness. For some people, such as the elderly and
those with underlying medical conditions, the flu can cause serious complications which require
hospitalisation. It can sometimes lead to death. Flu is usually spread through infected people coughing and
sneezing, which temporarily contaminates the surrounding air and surfaces with infected droplets
Signs and Symptoms
Symptoms usually appear 1–3 days after being infected. A person can spread flu to others 1–2 days before
they become unwell and up to 5 days after symptoms develop. The symptoms of influenza can include:
• fever
• dry cough
• muscle and joint pain
• tiredness or extreme exhaustion
• headache
• sore throat
• Stuffy nose
Prevention and treatment
1. Generally, uncomplicated flu is managed by simply resting in bed, drinking plenty of fluids (particularly
water) and taking over-the-counter medication to help relieve the symptoms.
2. Antiviral medications reduce the length of time symptoms last and help people infected return to their
daily routines earlier.
3. Good hygiene is essential to protect yourself and others
4. You can reduce the risk of infection by getting vaccinated.
Effect of smoking on the respiratory system
The respiratory system is not designed to cope with tobacco smoke. Tobacco smoke paralyses the cilia in the
respiratory tract and stops their movement.
In addition, tobacco smoke increases the production of mucus in the air passages. A cough by a smoker is an
attempt to remove the excess mucus from the respiratory system. Besides irritating the trachea and bronchi,
smoke particles interfere with the uptake of oxygen in the air sacs.
When cigarette smoke is inhaled, about one-third of the particles remain in the alveoli. Phagocytic cells
called macrophages can slowly remove many of the particles. However, an excess of particles from smoking
or from other sources of air pollution breaks down the walls of the air sacs and causes the formation of
inelastic tissue. This reduces the functional area of the respiratory surface and in severe cases may lead to a
disease called emphysema. In some cases, lung cancer also develops.
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Stomata are the main structures for gaseous exchange in leaves of plants. They are found in the epidermis of
leaves, with the exception of submerged plants, stomata are present in all the leaves of plants. Most land
plants have stomata on the lower surface of the leaf.
The guard cells control the opening and closing of each stoma.
When the stomata are open, air from the surrounding enters the leaf and occupies the air spaces. Oxygen and
carbon dioxide diffuse into or out of the leaf cells along their concentration gradient.
Gaseous exchange takes place in leaves. Exchange of gases in plants is required for two critical processes:
respiration and photosynthesis. Respiration in leaves takes place during the day as well as night, while
photosynthesis takes place in presence of light. The entire process can be studied as follows:
At the night, there occurs no photosynthesis, no O2 is produced. Oxygen from the air diffuses into the
leaves to carry out respiration. During respiration, CO2 is produced which diffuses out into the air. Thus,
the net exchange of gas in leaves at night is: O2 diffuses in and CO2 diffuses out.
During the day, CO2 (generated by respiration) is used up for photosynthesis. Hence, there is no release
of CO2. Instead, O2 is released. The leaves use some of this O2 for respiration and rest of the O2 diffuses
out into the air. So, the net exchange of gases in leaves during the day time is: CO2 diffuses in and O2
diffuses out.
Note that when air is entering or leaving the plant through stomata, the process is not gaseous exchange, but
diffusion. Gaseous exchange refers to the movement of gases between the cells and their surroundings.
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1. Which of the following is not true? Gaseous exchange in plants is through ............... .
a. Pneumatophores c. Xylem
b. Stomata d. Lenticels.
2. Which of the following is false? Pneumonia is caused by .............. .
a. Streptococcus pneumonia c. Virus
b. Fungi d. Protozoa
3. Which of the following respiratory diseases is caused by inhaling asbestos dust?
a. Tuberculosis b. Lung cancer
b. Lung cancer c. Asthma
4. Identify the importance of gaseous exchange.
5. Explain the characteristic features of gaseous exchange surfaces.
6. Describe the process of gaseous exchange in the alveoli. Use a diagram.
7. Discuss the causes, spread, treatment and prevention of the following respiratory diseases.
a. Tuberculosis
b. Lung cancer
c. Common cold
8. The diagram below shows a cross section through a green leaf.
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(a) Deamination
The amino acids absorbed in the gut are used in the synthesis of proteins. The body does not store excess
amino acids or proteins. The excess amino acids are broken down in the liver in a process called
deamination. From each amino acid, the amino group (NH2)) is converted to ammonia (NH3). Ammonia
should not be allowed to accumulate in the body because it is highly toxic. The remainder of the amino acid
molecule is changed to glycogen or fat for storage. The ammonia produced from the amino group is quickly
converted to a less toxic substance called urea.
(b) Detoxification
The liver removes harmful substances such as drugs and hormones from the blood. Within the liver, these
substances are converted into inactive or less dangerous forms, for instance, hydrogen peroxide, a highly
toxic by-product of certain metabolic process is rapidly split into water and oxygen by enzyme catalase in
the liver.
catalase
Hydrogen peroxide → water + oxygen
catalase
2H2O2 (aq) → 2H2O (l) + O2( g)
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Thus, the liver purifies or detoxifies blood. The inactive substances formed in the liver are returned to the
bloodstream and are finally excreted from the body by the kidneys.
A pair of kidneys
A pair of ureters
A urinary bladder
A urethra
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Ureter
A thin muscular tube called the ureter comes out of each kidney extending from the renal pelvis. It carries
urine from the kidney to the urinary bladder.
Urinary Bladder
It is a sac-like structure that stores urine until micturition. Micturition is the expulsion of urine from the
body. The urine is carried to the bladder through the ureters.
Urethra
This tube arises from the urinary bladder and helps to expel urine out of the body. In males, it acts as the
common route for sperms and urine. Its opening is guarded by a sphincter.
Nephron, urine formation and secretion
Urine is formed in small units called nephrons. Blood carrying excretory wastes enters the kidney through
the renal artery. Renal artery divides into capillaries forming the glomerulus.
The afferent arterioles entering the glomerulus are wider than veins leaving it (efferent). This
creates a high pressure of the blood in the glomerulus.
Ultrafiltration occurs and causes blood plasma, urea, water, salts, glucose, amino acids, etc… to
filter out through the capillary walls into the Bowman’s capsule. Blood cells and blood proteins
remain infiltrated. This ensures that kidney is selective filter, because it hold completely some
compounds and blood cells and let others completely or partially.
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The urine is formed in the nephrons and involves the following steps:
Glomerular filtration
Tubular reabsorption
Secretion
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The reabsorption
Reabsorption takes place in the proximal and distal convoluted tubule and the loop of Henle. The
glomerulus filtrate contains certain substances contain which are important to the body like such as
glucose, amino-acids, vitamins, fatty acids, water, Na+, Cl-, etc…
These essential substances are re-absorbed in the proximal and convoluted tubule and return back in
the blood stream.
Excessive salts, excessive water, urea, and inactive hormone form urine. It passes down the
collecting tubule, pelvis and through the ureter to the bladder where it is stored temporarily. The
sphincter muscles relaxes and urinary bladder contracts. The urine is then discharged from the
body through the urethra.
Function of excretion
To remove waste products from the body as soon as they are formed, before they can reach toxic and
harmful levels.
Removal of waste products that result from chemical reactions, which indirectly promotes continuous
breakdown of foods absorbed, and continued body reactions.
Sweating, which is part of excretion by the skin, helps in regulation of body temperatures on a hot day.
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Large intake of fluids lowers the osmotic pressure of blood. This leads to reduced reabsorption of water in
the kidney tubules resulting in the production of large amounts of dilute urine.
Intake of a salty meal raises the osmotic pressure of blood. This leads to increased reabsorption of water in
the kidney tubules resulting in the production of coloured, little and smelly urine.
3. Weather
In hot and dry weather conditions, the body tends to lose a lot of water through sweating thereby raising the
osmotic potential of blood. In this case a lot of water is reabsorbed resulting in coloured, little and smelly
urine. During cold weather the frequency of urination increases because sweating is so minimal.
4. Physical activity
During an exercise like running, jumping and playing, we sweat a lot. The kidney reabsorbs more water
resulting in little, coloured and smelly urine.
5. Diseases
Certain diseases that affect the secretion of hormones that control reabsorption of water in the kidney tubules
can either lead to production of large or small amounts of urine. An example is diabetes insipidus.
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b. Movable joints
These are joints that allow movement of body parts to take place. Some movable joints allow only a
small degree of movement while others allow a wide range of movement.
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2. Synovial joints
These joints are classified depending on the shape of the adjoining articular surface. They include:
vii. Saddle joints
Hinge joints are joints that allow back and forth movement as the only type of movement. Examples are
joints at the elbow, knee and fingers.
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v. ............... are elastic bands of fibres which connect two bones together. (ligaments/cartilages)
vi. .............. joint allows one part of the body to rotate over another. (ball and socket/pivot)
vii. The ............... and the triceps are antagonistic muscles. (biceps/forceps)
2. Which type of joints found in the elbow?
3. What is ligament?
4. What do you understand by antagonistic muscles? Give an example.
5. Complete the table below;
Type of joint Examples
Fixed joint
Sliding joint
Synovial joint
8. The diagram below shows a typical synovial joint. Study the diagram and answer the questions that
follow.
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The body has many ways of defending itself from invasion by harmful microorganisms. In most cases the
body’s defense mechanism prevents this from happening. Some of these defense mechanisms are made in a
way to block pathogens from entering into the body. Other mechanisms eliminate pathogens that are already
in the body. There are also defence mechanisms that attack micro-organisms when they persist inside the
body. The ability of the body to prevent itself from diseases using its lines of defense is called resistance.
Body defence mechanisms can either be first line or second line.
back and forth in a wave-like motion. Cilia trap microbes and dust particles and prevent them from
entering the lungs. These particles then combine with mucus and are either coughed, sneezed out or
swallowed and then passed out of the body in faeces.
(iv)The stomach produces hydrochloric acid which destroys many of the microbes that enter the body in
food and drinks we take.
(v) Tears contain a powerful enzyme that can digest and breakdown harmless substances. Therefore,
harmful microorganisms cannot enter the body through the opening of the eye.
(vi) Clotting of blood occurs when an open cut or wound exposes blood to air. Such a cut causes a break
in the skin exposing the body to harmful micro-organisms. When a clot forms, it seals the opening
and enables the wound to heal.
Immunity is the ability of an organism to resist a particular infection or toxin by the action of specific
antibodies or some white blood cells. An antibody is a protein produced by the body’s immune system
when it detects harmful substances, called antigens. Examples of antigens include micro-organisms
such as bacteria, fungi, parasites, viruses, and chemicals. Each type of antibody is unique and defends
the body against one specific type of antigen.
Antibodies detect and neutralises the antigen by binding to it. This signals the production of other cells
of the immune system to get rid of the invading microbes before it spreads or attacks the body.
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c) Tuberculosis
Tuberculosis (TB) is caused by bacteria called Mycobacterium tuberculosis that most often affects the lungs.
TB is spread from person to person through the air. When a person with pulmonary TB coughs, sneezes or
spits, they spread germs into the air. If a healthy person nearby inhales these germs they can become infected.
Signs and symptoms of pulmonary TB
i. Cough with sputum and blood at times
ii. Chest pains
iii. General weakness
iv. Weight loss
v. Fever
vi. Night sweats
d) Salmonellosis
This is an infection caused by Salmonella bacteria. The elderly, infants, and those with impaired immune
systems are more likely to develop severe form of illness. Salmonella infection usually results from
ingestion of the bacteria from contaminated food or water. Eggs, milk, meat or poultry are particularly high
risk foods. Fruit and vegetables may also be contaminated, especially if manure has been used as fertiliser.
People may become infected if they transfer animal faeces containing Salmonella bacteria from their hands
to their mouths, for example, eating after touching animals and failing to wash hands.
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e) Typhoid
Typhoid fever is caused by a bacterium called Salmonella typhi, which only lives in human.
The bacteria is spread by eating food or drinking water contaminated with faeces or urine of patients
and carriers. Typhoid is common in areas where hygiene standards are poor and water is likely to be
contaminated with sewage waste.
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(ii) Food handlers in institutions, like hospitals, schools and restaurants should go for regular
medical checkups and treatment if found infected.
(iii) Proper disposal of faeces in toilet and in pit latrines.
(iv) Water treatment and purification to kill the bacteria.
(v)Boiling drinking water and cooking of food properly to kill the bacteria.
(vi) Practice good hygienic methods like washing hands properly after visiting the toilet.
(vii) Washing fruits and vegetables with clean water before eating.
(viii) Vaccinations in the case of outbreaks of the disease in epidemics.
(vii) Seek proper treatment from qualified medical doctor in case of an infection.
Antibiotic treatment helps reduce the time a person is infectious.
f) HIV and AIDS
The Human Immunodeficiency Virus (HIV) attacks the immune system. This weakens a person’s defence
mechanism against infections and some types of cancer.
HIV and AIDS is transmitted from one person to another through various ways including:
(i) By birth, from an infected mother to her unborn baby.
(ii) By having unprotected sexual intercourse with an infected person.
(iii) (iii) By transfusion of infected blood.
(iv) By sharing of unsterilised surgical and skin piercing instruments with infected people.
Symptoms of HIV and AIDS
The symptoms depend on the stage of infection. People living with HIV and AIDS tend to be most infectious
in the first few months. The first few weeks after initial infection, individuals may experience no symptoms
or an influenza-like illness including fever, headache, rash or sore throat.
As the infection progresses, the immune system weakens. An individual can develop other signs and
symptoms, such as swollen lymph nodes, weight loss, fever, diarrhoea and cough. Without treatment, they
could also develop severe illnesses such as tuberculosis, meningitis (disease caused by bacteria and viruses
and its symptoms include fever, headache, nausea, blured vision and confusion) and cancers such as
lymphomas and Kaposi’s sarcoma (cancer that manifests itself as small, dark, raised bruise-like patches on
the skin, inside the mouth and the nose), among others.
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6. If there is an outbreak of cholera in your village, suggest preventive measures can you give to people.
7. Infectious diseases are deadly and sometimes fatal. They not only affect physically, mentally but also
economically. Discuss.
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Antigen
Antigen is any substance that is capable of stimulating an immune response, specifically activating the body
to produce antibodies.
There are two main divisions of antigens: foreign antigens and self-antigens.
• Foreign antigens come from outside the body. All pathogens (viruses, bacteria and protozoa, and certain
proteins in foods) are examples of foreign antigens.
• Self-antigens come from within the body. Normally, the body is able to distinguish self from non-self.
However, in individuals with autoimmune disorders, normal body substances provoke an immune
response, leading to the generation of auto-antibodies.
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Antigen–antibody reaction
An antigen and antibody reaction works like a lock and key mechanism. Antibodies themselves do not
destroy antigens. They inactivate and tag antigens for destruction by phagocytes.
All antibodies form an antigen-antibody (immune) complex When they combine with antigens.
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a) Innate immunity
Innate immunity also called nonspecific immunity is the body defense system that one is born with. It
protects against all antigens Innate immunity involves barriers that keep harmful materials from entering
your body. It consists of cells and proteins that are always present and ready to fight microbes at the site of
infection.
The main components of the innate immune system are:
v. Physical epithelial barriers
vi. Phagocytic leukocytes
vii. Dendritic cells
viii. A special type of white blood cells called a natural killer (NK) cell
ix. Circulating plasma proteins
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Innate immunity includes the components of the body’s first line of defence. These are enzymes in tears and
skin oils, mucus, skin and acid in the stomach. If an antigen gets past these barriers, it is attacked and destroyed
by other parts of the immune system.
b) Adaptive immunity
Adaptive immune system also known as acquired immunity is immunity that develops after exposure to
various antigens. The immune system builds a defense against that specific antigen. Components of the
adaptive immune system are normally silent; however, when activated, these components “adapt” to the
presence of infectious agents by creating mechanisms for neutralising or eliminating the microbes.
The immune system includes certain types of white blood cells, for example, lymphocytes. There are B and
T type lymphocytes.
• B lymphocytes are cells that produce antibodies. Antibodies attach to a specific antigen and present it
to other immune cells for destruction.
• T lymphocytes are cells that attack antigens directly and help control the immune response.
During growth and development of white blood cells, they always learn to differentiate between individuals
own body tissues and foreign substances. Once B cells and T cells are formed, a few of those cells will multiply
and provide "memory" for the immune system. This allows the immune system to respond faster and more
efficiently the next time an individual is exposed to the same antigen. In many cases it will prevent you from
getting sick, for example, a person who has had measles or has been immunised against measles is immune
from getting it again.
Active immunity
This is the immunity that results from the production of antibodies by the immune system in response to the
presence of an antigen. Active immunity is either natural or artificial.
• Naturally acquired active immunity occurs when a person is exposed to a live pathogen. The individual
develops the disease, and becomes immune as a result of the primary immune response.
• Artificially acquired active immunity can be induced by a vaccine, a substance that contains alternated
form of the antigen. A vaccine stimulates a primary response against the antigen without causing
symptoms of the disease.
Passive immunity
This is a short-term immunity that results from the introduction of antibodies from another person or animal.
Passive immunity is either artificial or natural.
Artificially acquired passive immunity is a short-term immunisation. It is achieved by the injection
of antibodies that are not produced by the recipient's cells.
• Naturally acquired passive immunity occurs during pregnancy, in which certain antibodies are
passed from the maternal body into the foetal bloodstream.
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in nutrients and antibodies. These antiodies protect against bacterial infections, until the newborn can
synthesise its own antibodies. Maternal antibodies protect against some diseases more than other artificial
antibodies. Maternal passive immunity offers immediate protection which lasts four to six months after birth.
For this reason, breastfeeding a baby for at least one thousand days after birth is very important.
13.5 Immunisation and autoimmunity
Immunisation (vaccination) is the process whereby a person is made immune or resistant to an infectious
disease, typically by the administration of a vaccine. Small amounts of an antigen, such as dead viruses, are
given to activate immune system "memory". Memory allows your body to react quickly and efficiently to
future exposure. Such small doses of antigens are referred to as vaccines (a vaccine is a substance used to
stimulate the production of antibodies and provide immunity against one or several diseases, prepared from
the causative agent of a disease, its products, or a synthetic substitute, treated to act as an antigen without
inducing the disease).
Autoimmunity is a condition whereby the immune system attacks and kills own cell. The immune system
normally guards against germs like bacteria and viruses. When the immune system cells start to mistakenly
attack and destroy the body’s healthy tissue just as if it were a foreign invader, like bacterim or virus, is called
autoimmune response. An autoimmune disease is a condition in which your immune system mistakenly
attacks your body.
Complications from altered immune responses include:
i. Allergy or hypersensitivity
ii. Autoimmune disorders
iii. Immunodeficiency disorders
iv. Serum sickness
v. Transplant rejection
Type 1 diabetes
Type 1 diabetes was initially known as juvenile diabetes or insulin-dependent diabetes mellitus. It is a chronic
condition in which the pancreas produces little or no insulin. Insulin is a hormone needed to enable sugar
(glucose) to enter cells to produce energy.
Causes
The exact cause of type 1 diabetes is unknown. Usually, the body's own immune system which normally
fights harmful bacteria and viruses mistakenly destroys the insulin-producing cells (beta cells in islets of
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Langerhans) in the pancreas. Other possible causes include: genetics and exposure to viruses and other
environmental factors.
Type 1 diabetes signs and symptoms can appear relatively suddenly and may include:
Increased thirst
Frequent urination
Bed-wetting in children who previously didn't wet the bed during the night
Extreme hunger
Unintended weight loss
Irritability and other mood changes
Fatigue and weakness
Blurred vision
14.1 Introduction
Sexual behaviour refers to the way in which people experience and express their sexuality. Sexuality refers
to a person’s sexual orientation or preference and the capacity of having sexual feelings. Sexual response
refers to the physical and emotional changes that occur as a person becomes sexually aroused and
participates in sexually stimulating activities.
14.2 Male and female sexual response
The human sexual response is a series of changes that take place in the body in response to sexual
stimulation. These changes take a different form in men and women. Sexual response occurs when there is
sexual stimulation (any stimulus whether physical or mental that leads to sexual arousal) such as through
touch, looking, smelling or thoughts.
Sexual responses in male
• There is an erection: the penis becomes large and hard.
• There is an increase in the size of the testes.
• The nipples may become hard.
• The heart beat is faster and breathing rate increases.
• The muscles contract.
Female sexual responses
• There is a nice and warm feeling all over the body.
• The hart beat and breathing rate increases.
• The pupils become bigger.
• The breasts become larger and nipples get hard.
• The vagina becomes lubricated.
• The clitoris sweels slightly and hardens.
• The muscles in the vagina contract.
These changes take a different form in men and women. The stages are in four phases as below.
i. Excitement phase
ii. Plateau phase
iii. Orgasmic phase
iv. Resolution phase
1. Excitement phase
Excitement is the initial stage of stimulation that gets the body ready for sex. The stimulation may be due to
kissing, seeing an attractive person or soft touches. This stage is accompanied by a rise in heart rate,
breathing rate and blood pressure. The phase can last for a few minutes to several hours.
2. Plateau phase
This is a phase that prepares both man and woman for orgasm. It is characterised by increased heartbeat,
muscle tension and blood pressure.
3. Orgasmic phase
The orgasm is the climax (peak) of the sexual pleasure which occurs during sexual activity and which
In males may include ejaculation and in females vaginal contractions. It is the shortest of the phases and
generally lasts only a few seconds. General characteristics of this phase include: Blood pressure, heart rate
and breathing which are at their highest rates, accompanied by a rapid intake of oxygen. This phase is
accompanied with sexual pleasure and satisfaction with release of sexual tension.
4. Resolution phase
During resolution, the body slowly returns to its normal level of functioning. This phase is marked by a general
sense of well-being, enhanced intimacy and, often, fatigue.
Puberty in girls
On average, girls begin puberty at an early age of about 10-11years. Puberty in girls occurs fast as compared
to boys. Menstruation in girls is a characteristic that shows the onset of puberty. This is brought about by the
growth and development of the ovaries.
Early onset of puberty in girls is detrimental, causing low self-esteem and stress. This is due to breast
enlargement and a lot of fat deposition on the body that causes different body shapes between the girl and her
peers. Early onset is caused by a number of factors which include diet and environmental exposure.
Puberty in girls is more challenging to parents. Girls tend to become more private and sensitive emotionally.
Parents should therefore be more careful. Social behaviours of girls also tend to change. Girls become more
attracted to males than females. The main challenge that girls face during puberty is unwanted pregnancies.
Other challenges include rejection from peers, clothes to wear and physical appearance.
b) Functional disorders: This is the inability of a woman to experience sexual pleasure and satisfaction. This
results from emotional problems, feeling of guilt and trauma.
c) Difficulties getting aroused: this is one of the major problems experienced by women. Some women do not
get aroused easily during sexual stimulation. This may be caused by stress, anxiety or sex trauma.
Sexual behaviours are based on the morals and values of the particular society. These norms help to govern
the sexual behaviours of individuals in a society. These norms are passed across people in a society by
family members, teachers in schools, religious leaders, peers and the media.
The following sexual behaviours are unacceptable in the rwandan culture:
i) Incest: This is the act of having sex with a close relative.
ii) Homosexuality: This is where people of the same sex are engaged in a sexual relationship.
iii)Masturbation: The act of selfstimulation that leads to sexual satisfaction.
iv) Anal sexual intercourse: This is a type of sexual activity in which the penis is inserted into the anus.
v) Rape: The act of forcing some one into sexual intercourse without consent.
vi) Adultery: Having sex outside wedlock.
vii) Fornication: Engaging in sex before marriage.
viii) Sex slavery: The act of taking advantage of vulnerable individuals in a forced commercial sex.
ix) Prostitution: Engaging in sexual relationships for financial gains.
Ageing is process of growing old. The ageing process does not reduce the need or desire for sex. For many
older adults, the concept of sexuality includes practices such as kissing, hugging and fondling. Such activity
may replace actual intercourse and becomes increasingly important for those who no longer desire or are in
capable of sexual intercourse.
Ageing in men
In an ageing male, the most common sexual dysfunction is erectile dysfunction. This can be due to hormonal
changes as part of normal ageing or due to underlying conditions such as diseases. Testosterone hormones
which increase sexual urge in men decline with age.
Ageing in women
The most common sexual dysfunction in women, is lack of desire and arousal. This is due to decrease in
hormones, which begins at menopause and continue as women age. This decrease in hormones may also
produce reduced muscles, loss of bone mass and declining energy. Sexually, it may result in vaginal dryness,
decreased libido, and decreased ability to achieve orgasm.
1. Every person who occupies accommodation has a right to freedom from harassment because of sex,
sexual orientation, gender identity or gender expression by the landlord or agent of the landlord or by
an occupant of the same building.
2. Every person who is an employee has a right to freedom from harassment in the work place because
of sex, sexual orientation, gender identity or gender expression by his or her employer or agent of the
employer or by another employee.
3. Every person has a right to be free from a sexual solicitation or advance made by a person in a
position to confer, grant or deny a benefit or advancement.
Ways in which human sexual rights are violated
1. Rape: penetration of the vagina or anus with the penis, other body part or foreign object without
consent (willingnes). It also includes forced oral sex.
2. Human trafficking for sexual exploitation: this is where people are kidnapped and sold to other
people for sexual exploitation. Sex trafficking or slavely is the exploitation of women and children,
within national or across international borders, for the purposes of forced sex work. Commercial
sexual exploitation includes pornography, prostitution and sex trafficking of women and girls, and is
characterized by the exploitation of a human being in exchange for goods or money.
3. Marriage /de facto rape: de facto refers to something that exist in fact, although perhaps not
intended, legal or accepted. Marriage rape occurs when one intimate partner pressures or forces the
other partner into having sex. This is rape, even though the person is a spouse or regular sexual
partner.
4. Child sexual assault: is any act of a sexual nature towards a child by someone older than the child or
in a position of power.
5. Incest: is sexual assault by a family member. This may be a parent, sibling, step-parent, uncle or any
other family member. Incest is a taboo in rwandan culture.
c) Temperature method
Temperature method is also known as the basal body temperature method. It is a type of natural family
planning where a woman measures her basal body temperature: temperature when fully at rest especially after
waking up. It is believed that ovulation may cause a slight increase in basal body temperature. Elevated waking
temperature for three days in a row is considered confirmation of the post-ovulatory less fertile phase.
d) Calendar method
Calendar-based methods are various methods of estimating a woman's likelihood of fertility, based on a
record of the length of previous menstrual cycles. Calendar based contraceptive methods prevent
pregnancy by monitoring the fertile periods during the menstrual cycle. To use this method a woman
monitors the length of her menstrual cycle for a period of six months to establish how many days her cycle
usually lasts for.
Advantages
It allows a woman to understand her fertile and infertile days and helps to safeguard the woman’s
reproductive health.
Disadvantages
It is not an effective method since the body system can fail.
Advantages of natural contraception
a) Effective method of birth control.
b) Have no negative health side effects.
c) An alternative for women who cannot or do not want to use hormonal methods.
d) Promotes positive body awareness.
e) Consistent with many religious beliefs and lifestyles.
f) Alerts women to reproductive health and fertility concerns.
g) Fosters communication between partners and encourages male involvement.
Disadvantages of natural contraception
a) Provides no protection from sexually transmitted infections.
b) Often difficult to find trained instructors.
c) Requires time to learn (usually 3 to 6 cycles).
d) Requires discipline and commitment to chart fertility signs and follow the rules to avoid pregnancy.
e) Times of abstinence from intercourse may be a challenge for some couples
Artificial birth control is any product, procedure or practice that uses artificial means to prevent pregnancy.
Barrier methods such as condoms and diaphragms, hormonal methods such as the pill and, and surgical
sterilisation procedures such as vasectomy or hysterectomy are all artificial birth control methods. Most
types of artificial contraceptives work by:
• Preventing an egg from being released every month (hormones).
• Preventing sperms from reaching the egg (barrier and some IUD methods).
• Blocking the reproductive function in men or women (sterilisation).
• Preventing a fertilised egg from implanting in the uterus (hormones).
Barrier methods
a) The male condom
This is a thin rubber sheath worn by a man over an erect penis. It collects semen at its tip so that they are
not released into the vagina. Condoms are usually made of latex. It is the most used contraceptive.
c) Spermicide
Spermicide is a birth control method that contains
chemicals that stop sperms from fertilising the ova.
Spermicides are available in different forms, including
creams, film, foams and gels. Spermicide can be used
alone, or it can be used with other birth control methods
such as diaphragm, to make them more effective.
Spermicide does not always offer the best protection
against pregnancy. It has very few associated side-
effects, but it does not protect against STIs.
a) The Pill
It is the most popular form of female
contraception. It is taken orally in form of
a tablet, every day at almost the same
time. The contraceptive pill will prevent
pregnancy 95%.
It comes close to providing 99%
protection if one pill is taken every day as
prescribed. The pill does not provide any
protection against STIs and a doctor's
prescription is required to buy it.
f) Emergency contraception
The effectiveness rate for IUDs is above 99%. However, it provides no protection against STIs. It requires a
doctor to properly insert it and to make periodic follow ups.
Figure
15.13: Vasectomy
• Non-surgical sterilisation: This involves placing a coil in each fallopian tube through the vagina and
uterus. Scars appear and eventually block each tube completely. The scars may take up to 3 months to
completely block the tubes, so you need to use another method of contraception in the meantime.
Both options also offer more than 99% of protection against pregnancy. In rare cases the blocked tubes can
grow back and reconnect. However the method offers no protection against STIs.
15.5 Abortion
Abortion is the deliberate process of prematurely terminating a pregnancy usually before the embryo or foetus
is capable of independent life. Depending on the number of weeks it has, a pregnancy can be terminated either
by taking medication or through a surgical procedure.
The following are some of the reasons why a woman might decide to have an abortion:
• Personal circumstances including risk to the wellbeing of existing children.
• Health risk to the mother.
• A high chance that the baby will have a serious abnormality either genetic or physical.
15.6 Menopause
This is a term used to describe that state when a woman stops having monthly periods and is no longer able
to get pregnant naturally. Monthly periods become less frequent over a few months or years before they stop
altogether. In some women they stop abruptly.
Most women will experience menopausal symptoms. Some of these can be quite severe and have a significant
impact on a woman’s everyday activities. Common symptoms include:
a. Hot flushes
b. Night sweats
c. Vaginal dryness and discomfort during sex
d. Difficulty in sleeping
e. Low mood or anxiety
f. Reduced sex drive (libido).
g. Problems with memory and concentration
Menopausal symptoms can begin months or even years before the periods stop. They can last around four
years after the last period, although some women experience them for much longer.
Makes education for the children more affordable, and better educated children can then take better
care of their parents later in life
Reduces maternal and child illnesses, allowing money that would otherwise be used on their
treatments to be used for constructive purposes. Morevoer, if the mother is healthy, she can participate
in economic activites
In males
• Pain when passing out urine.
• White discharge from the penis.
• The testicles may be painful or swollen.
• Swelling of skin around the anus.
In females
• Painful and frequent urination.
• Smelly yellowish and abnormal vaginal discharge.
• Pain in the lower abdomen.
• Swollen skin in the vagina or around the anus.
Treatment
Chlamydia is easily treated using antibiotics.
2. Gonorrhoea (imitezi)
Gonorrhoea is caused by a bacterium called Neisseria gonorrhoeae. Gonorrhoea is transmitted through
sexual contact with the penis, vagina, mouth or anus of an infected partner. Gonorrhoea can also be spread
from mother to baby during childbirth.
Signs and symptoms of gonorrhoea
Some men with gonorrhea may have no symptoms at all. However, men who do have symptoms may have:
• A burning sensation when urinating.
• A white, yellow, or green discharge from the penis.
• Painful or swollen testicles (although this is less common).
Most women with gonorrhea do not have any symptoms. Even when a woman has symptoms, they are often
mild and can be mistaken for a bladder or vaginal infection. Women with gonorrhea are at risk of developing
serious complications from the infection, even if they do not have any symptoms. Symptoms in women can
include:
• Painful or burning sensation when urinating.
• Increased vaginal discharge.
• Vaginal bleeding between periods.
Treatment
Gonorrhea can be treated using antibiotics like penicillin.
3. Syphilis (mburugu)
Syphilis is caused by a bacteria called Treponema pallidum. It is transmitted from person to person by direct
contact with a syphilitic sore, known as a chancre. Chancres occur mainly on the external genitals, vagina,
anus or in the rectum. Chancres also can occur on the lips and in the mouth. Transmission of syphilis occurs
during vaginal, anal or oral sex.
The bacterial infection progresses through several stages.
• In the primary stage, small hard painless sores develop at the site of infection usually the penis and
the vagina.
• The disease enters secondary stage several weeks later characterised by rashes on the skin and mild
fever. These symptoms subside after a few weeks followed by a latent asymptomatic period.
• In the tertiary stage, lesions develop and cause extensive tissue damage that may lead to paralysis,
insanity, blindness and eventually death.
• Treatment
• Antibiotics like penicillin, erythromycin or tetracycline are used to treat syphilis although some
strains can be resistant to certain antibiotics.
16.2 Transmission of HIV (Human immunodeficiency virus) infection
HIV is the virus that causes AIDS (Acquired immune deficiency syndrome). It is mainly transmitted
through:
a. Having unprotected sex (meaning sex without a condom) with someone who has HIV, particularly
unprotected vaginal sex and anal sex.
b. Sharing injecting equipment like needles, syringes or other equipment with someone who has HIV.
c. Infected mother-to-baby during pregnancy, childbirth and breastfeeding
d. Contaminated blood transfusions and organ/tissue transplants (this risk is extremely small because
most countries test blood products for HIV first.)
e. Being bitten by a person with HIV. There is no risk of transmission if the skin is not broken.
f. Deep, open-mouth kissing if both partners have sores or bleeding gums.
g. Contact between broken skin, wounds or mucous membranes and HIV-infected blood or
bloodcontaminated body fluids.
h. Eating food that has been pre-chewed by an HIV-infected person. The only known cases are among
infants.
1. Bacterial STIs can be cured using antibiotics if treatment begins early enough. Antibiotics, often in a
single dose, can cure many sexually transmitted bacterial and parasitic infections, including gonorrhoea,
syphilis, chlamydia and trichomonasis. Treatment of gonorrhoea and chlamydia is always done at the same
time because the two infections often appear together.
2. Viral STIs cannot be cured, but the symptoms can be managed with medications. For instance there is
a vaccine against hepatitis B, but it will not help if you already have the disease.
HIV cannot be cured but can be managed. A person may choose to visit a voluntary counselling and testing
(VCT) centre to undergo HIV and AIDS counselling. After conselling he or she can make an informed
decision about whether to be tested for HIV or not.
At the VCT centre pre-test and posttest counselling is offered to assist people to learn more about their
status. Counselling involves getting information and learning how to live positively with the virus. This
means learning to accept the fact that one is HIV-infected, seeking emotional support, eating a healthy diet,
learning how to control the amount of stress in our life, making sure there is no re-infection and planning for
the future.
Antiretroviral therapy (ART) is the use of HIV medicines to treat HIV infection. People on ART take a
combination of HIV and AIDS drugs every day. People infected with HIV and AIDS should start ART as
soon as possible. ART cannot cure HIV and AIDS, but helps people infected to live longer and healthier lives.
ART also reduces the risk of HIV and AIDS transmission.
Potential risks of ART include unwanted side effects from HIV and AIDS drug interactions. This includes not
taking drugs every day as required. It could lead to drug resistance and treatment failure.
Glossary
Abiotic - physical factors in the environment that affect living organisms. They include; wind, humidity,
sunlight, soil and atmospheric pressure among others.
Abortion - deliberate physical or chemical termination of human pregnancy.
Aboral - relating to or denoting the side or end that is furthest from the mouth, especially in animals that
lack clear upper and lower sides, such as echinoderms.
Abstinence - act of restraining oneself from doing something; for example, restraining oneself from having
sex.
Active transport - movement of particles against a concentration gradient.
Active site- a region on an enzyme that binds to a protein or other substance during a reaction.
Aerencyma tissue - A spongy tissue with large intercellular air spaces that is found in aquatic plants. It
provides buoyancy and allows the circulation of gases.
Aerosol - a substance enclosed under pressure and able to be released as a fine spray.
Agglutination - the clumping together in suspension of antigen-bearing cells, micro-organisms or particles
in the presence of specific antibodies.
Amenorrhea - absence of menstruation for at least three months
Appendage - a structure that attaches to another part of the body.
Antagonistic muscles - a muscle that opposes the action of another, for example, the biceps and triceps.
Antibody - proteins found in plasma that are responsible for mediation of immunity in body fluids.
Antibiotic - a drug derived from bacteria or fungus and is used to treat bacterial infections.
Anti-retroviral (ARV) - drugs to maximally suppress the HIV virus and stop the progression of HIV
disease.
Antigen - any substance that stimulates response of a specific immune system.
Articulate - meet at a point to form a joint.
Arthritis – a condition that causes pain and inflammation in a joint.
Autoimmune - a condition arising from an abnormal immune response to a normal body part.
Biotic - activities of living organisms that affect an ecosystem.
Bilateral symmetry - symmetrical arrangement of an organism or part of an organism along a central axis.
Bilirubin - a yellow pigment produced from the breakdown of heme in the worn out red blood cell.
Biodiversity - variety and variability of life forms on earth.
Biome - large ecological areas on the earth's surface, with fauna and flora (animals and plants) adapting to
their environment.
Biuret test- a test that uses a reagent (a solution of copper sulfate (CuSO4) and sodium hydroxide (NaOH))
used to determine the presence of peptide bonds in protein.
Bowman’s capsule - the filtration unit of the glomerulus and has tiny slits in which filtrate may pass
through into the nephron.
Bone – a rigid body tissue consisting of cells compressed into hard intercellular material.
Capillarity - the ability of a narrow tube to draw a liquid upwards against the force of gravity.
Carrier protein - a protein that transports specific substance across the cell membrane.
Cartilage - tough elastic tissue.
Catalyst - a substance that causes a chemical reaction to occur but is not itself involved in the reaction.
Cephalothorax – a term referring to the fused head and thorax occurring in many arthropods, particularly
crustaceans and arachnids.
Chaetae - bristles made of chitin that are found on bodies of annelids.
Cholesterol - a fatty substance made in the body and found in certain foods. Some cholesterol are harmful
others are useful to the body.
Carapace - hard structure made of chitin that covers the dorsal part of an animal and protects internal
organs. CD4 cell - white blood cells that play an important role in the immune system.
References
1. Godfrey Wasswa, Benson Mugarura, Selah Ndiwa (2018). Biology and Health Sciences for Rwanda
Schools Senior Two. Longhorn publishers limited.
2. https://www.avert.org/hiv-transmission-prevention/how-you-get-
hiv?gclid=EAIaIQobChMIjavPvrjb6AIVB0TTCh0w2AgeEAAYASAAEgKdxPD_BwE
3. https://www.fhi360.org/sites/default/files/media/documents/need-family-planning-handouts.pdf
4. https://www.who.int/news-room/fact-sheets/detail/family-planning-contraception
5. Juliet Mbogori,V.O.K Muranga, James Magodu (2017). Achievers Biology for Rwandan Schools
senior2. East African Educational Publishers Ltd.
6. Michael Roberts (1986); Biology - A Functional Approach (Fourth Edition); Oxford publishers
7. Nyiramuhire Dancilla, Ugirimbabazi Odax, Wat Tyler and Cary Grant (2017). Comprehensive Biology
and Health Science for Rwanda Schools student’s book 2. Laxmi Publications Pvt.Ltd.
8. www.healthline.com