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    Bacteriology

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    Hanna Marae
    The document summarizes the procedure for using the Bact/Alert 3D blood culture system. Key steps include: 1) Inspecting culture bottles for defects before inoculating with blood or body fluids drawn directly into the bottle. 2) Placing inoculated bottles into the Bact/Alert machine for incubation and automated monitoring every 10 minutes. 3) The machine will signal positive bottles based on changes in fluorescence from bacterial growth and metabolism producing carbon dioxide.

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    Bacteriology

    Uploaded by

    Hanna Marae
    43 views1 page
    The document summarizes the procedure for using the Bact/Alert 3D blood culture system. Key steps include: 1) Inspecting culture bottles for defects before inoculating with blood or body fluids drawn directly into the bottle. 2) Placing inoculated bottles into the Bact/Alert machine for incubation and automated monitoring every 10 minutes. 3) The machine will signal positive bottles based on changes in fluorescence from bacterial growth and metabolism producing carbon dioxide.

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    The document summarizes the procedure for using the Bact/Alert 3D blood culture system. Key steps include: 1) Inspecting culture bottles for defects before inoculating with blood or body fluids drawn directly into the bottle. 2) Placing inoculated bottles into the Bact/Alert machine for incubation and automated monitoring every 10 minutes. 3) The machine will signal positive bottles based on changes in fluorescence from bacterial growth and metabolism producing carbon dioxide.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    43 views1 page

    Bacteriology

    Uploaded by

    Hanna Marae
    The document summarizes the procedure for using the Bact/Alert 3D blood culture system. Key steps include: 1) Inspecting culture bottles for defects before inoculating with blood or body fluids drawn directly into the bottle. 2) Placing inoculated bottles into the Bact/Alert machine for incubation and automated monitoring every 10 minutes. 3) The machine will signal positive bottles based on changes in fluorescence from bacterial growth and metabolism producing carbon dioxide.

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    of 1

    WEEK 3: BACTERIOLOGY SECTION LEC 2: BLOOD CULTURE SYSTEM

    BACT/ALERT 3D PROCEDURE
    INTENDED USE:
    1. Remove the flip-off cap from the Bact/Alert vial
     BACT/ALERT 3D top and inspect the vials for cracks
    o Blood contamination, excessive cloudiness and bulging
    o Sterile body fluids or indented stoppers. (do not use if any defect is
    noted.)
    PRINCIPLE 2. Before inoculating, swab the septum with alcohol
    (iodine is not recommended). Aseptically inject
     BACT/ALERT Microbial Detection System utilize or draw the required amount of blood directly.
    a colorimetric sensor and reflected light to 3. Inoculated aerobic vials should be placed in the
    monitor the presence and production of carbon Bact/Alert instrument as soon as possible for
    dioxide (CO2) that is dissolved in the culture incubation and monitoring. If placement of
    medium. If microorganisms are present in the theinoculated vial into the instrument has been
    test sample, carbon dioxide is produced as the delayed and visible growth is apparent, it should
    organisms metabolize the substrates in the not be tested in the Bact/Alet instrument. It
    culture medium. When growth of the should be subcultured, gram stained and treated
    microorganisms produces CO2, the color of the as presumptive positive.
    gas-permeable sensor installed in the bottom of 4. Vial entered into the instrument will be
    each culture bottle changes to yellow. automatically tested every 10 minutes for the
    MATERIALS duration of testing. Positive vials will be
    determined by the Bact/Alert instrument and
     BACT/ALERT CULTURE BOTTLES identified as such. The sensor inside the bottle
     BACT/ALERT 3D SYSTEM MACHINE will not appear visibly different in positive and
    negative vials; The Bact/Alert however can
      determine differencein fluorescene.
    5. If at the end of the testing period, a negative vial
     
    appears visibly positive (i.e., chocolatized blood,
      bulging septum, lyzed and/or much darkened
      blood) it should be subcultured, gram stained
      and treated as presumptive positive.
      6. Positive vials should be subcultured on BAP,
      MacConkey, Mannitol salt and Chocolate agar.
      INTERPRETATION
     
     Positive or negative culture bottles are
    determined by decision making software
    contained in Bact/Alert microbial detection
    system. No action is required until the Bact/Alert
    instrument signals culture bottles positive or
    negative.

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