May 2017

DNeasy® PowerSoil® Kit

Handbook

For the isolation of microbial genomic DNA

from all soil types

Sample to Insight__
Contents
Kit Contents ............................................................................................................... 3

Storage ..................................................................................................................... 4

Intended Use .............................................................................................................. 4

Safety Information ....................................................................................................... 5

Quality Control........................................................................................................... 5

Introduction ................................................................................................................ 6

Principle and procedure......................................................................................... 6

Equipment and Reagents to Be Supplied by User .......................................................... 10

Important Notes ........................................................................................................ 10

Protocol: Experienced User ........................................................................................ 11

Protocol: Detailed ..................................................................................................... 13

Protocol: QIAvac 24 Plus Vacuum Manifold ................................................................. 17

Troubleshooting Guide .............................................................................................. 19

Ordering Information .................................................................................................... 21

2 DNeasy PowerSoil Kit Handbook 05/2017

Kit Contents
DNeasy PowerSoil Kit (50) (100)

Catalog no. 12888-50 12888-100

Number of preps 50 100

MB Spin Columns 50 2 x 50

PowerBead Tubes, Garnet 50 2 x 50

Solution C1 6.6 ml 6.6 ml

Solution C2 15 ml 2 x 15 ml

Solution C3 15 ml 2 x 15 ml

Solution C4 72 ml 2 x 72 ml

Solution C5 30 ml 2 x 30 ml

Solution C6 9 ml 2 x 9 ml

Collection Tubes (2 ml) 4 x 50 8 x 50

Quick Start Protocol 1 1

DNeasy PowerSoil Kit Handbook 05/2017 3

Storage
The DNeasy PowerSoil Kit reagents and components can be stored at room temperature
(15–25°C) until the expiration date printed on the box label.

Intended Use
All DNeasy products are intended for molecular biology applications. These products are not
intended for the diagnosis, prevention or treatment of a disease.

All due care and attention should be exercised in the handling of the products. We
recommend all users of QIAGEN products to adhere to the NIH guidelines that have been
developed for recombinant DNA experiments, or to other applicable guidelines.

4 DNeasy PowerSoil Kit Handbook 05/2017

Safety Information
When working with chemicals, always wear a suitable lab coat, disposable gloves and
protective goggles. For more information, please consult the appropriate safety data sheets
(SDSs). These are available online in convenient and compact PDF format at
www.qiagen.com/safety where you can find, view and print the SDS for each QIAGEN kit
and kit component.

WARNING: Solution C5 contains ethanol. It is flammable.

WARNING: Do not use bleach to clean the inside of the QIAvac® 24 Plus manifold.

CAUTION
DO NOT add bleach or acidic solutions to directly to the sample
preparation waste

PowerBead Tubes and Solution C4 contain guanidine salts, which can form highly reactive
compounds when combined with bleach. If liquid containing these buffers is spilt, clean with
a suitable laboratory detergent and water. If the spilt liquid contains potentially infectious
agents, clean the affected area first with laboratory detergent and water, and then with 1%
(v/v) sodium hypochlorite.

Quality Control
In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of
DNeasy PowerSoil Kits is tested against predetermined specifications to ensure consistent
product quality.

DNeasy PowerSoil Kit Handbook 05/2017 5

Introduction
The DNeasy PowerSoil Kit comprises a novel and proprietary method for isolating genomic
DNA from environmental samples using patented Inhibitor Removal Technology® (IRT). This
kit is intended for use with environmental samples containing high humic acid content,
including difficult soil types such as compost, sediment and manure. Other more common
soil types have also been used successfully with this kit. The isolated DNA has a high level of
purity, which allows for more successful PCR amplification of organisms from the sample.
PCR analysis has been performed to detect a variety of organisms including bacteria (e.g.,
Bacillus subtilis, Bacillus anthracis), fungi (e.g., yeasts, molds), algae and actinomycetes
(e.g., Streptomyces).

Principle and procedure

The DNeasy PowerSoil Kit uses a humic substance/brown color removal procedure. This
procedure is effective at removing PCR inhibitors from even the most difficult soil types.
Environmental samples are added to a bead beating tube for rapid and thorough
homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic
DNA is captured on a silica membrane in a spin column format. DNA is then washed and
eluted from the membrane. The isolated DNA is ready for PCR analysis and other
downstream applications.

Bead beating options

The DNeasy PowerSoil Kit does not require homogenization using a high velocity bead
beater. However, if the microorganism of interest requires stronger homogenization than
provided by a vortex, or if using a bead beater is desired, the DNeasy PowerSoil Kit may be
used in conjunction with the PowerLyzer® 24 Homogenizer (110/220V) (cat. no. 13155).
We now offer DNeasy PowerLyzer PowerSoil Kits (cat. no. 12855-50 and 12855-100) with
bead tubes suitable for high-powered bead beating of soil. For more information about these

6 DNeasy PowerSoil Kit Handbook 05/2017

products, or for references using the DNeasy PowerSoil Kit with other bead mill instruments,
please contact Technical Support at support.qiagen.com.

The PowerLyzer 24 Homogenizer (110/220V): Optimized for complete

homogenization of any sample

The PowerLyzer 24 Homogenizer (110/220V) is a bead beating instrument uniquely

designed for the most efficient and complete lysis and homogenization of biological
samples. In as little as 30 seconds, the PowerLyzer 24 Homogenizer is capable of
processing up to 24 samples in 2 ml tubes. With true ‘hands-free’ operation, the downtime
associated with manipulating samples through multiple cycles is eliminated. Even the
toughest and most difficult samples such as pine needles, seeds, spores, fungal mats and
clay soils are easily and effectively lysed. For more information and protocols, please
contact Technical Support at support.qiagen.com.

High-throughput options

We offer a vacuum-based protocol for faster processing without centrifugation for the
DNA-binding and column-washing steps for MB Spin Columns. The QIAvac 24 Plus
Manifold allows for processing of up to 24 MB Spin Column preps at a time. For additional
high-throughput options, we offer the DNeasy PowerSoil HTP 96 Kit for processing up to
2 x 96 samples using a centrifuge capable of spinning two 96 well blocks stacked
(13 cm x 8 cm x 5.5 cm) at 2500 x g. For 96-well homogenization of soil, we offer the
TissueLyser II and Plate Adapter Set (cat. no. 85300 and 11990, respectively.)

Automated nucleic acid purification on the QIAcube

Purification of DNA using the DNeasy PowerSoil Kit can be automated on the QIAcube®.
The innovative QIAcube uses advanced technology to process QIAGEN spin columns,
enabling seamless integration of automated, low-throughput sample prep into your
laboratory workflow. Sample preparation using the QIAcube follows the same steps as the

DNeasy PowerSoil Kit Handbook 05/2017 7

manual procedure (i.e., lyse, bind, wash and elute), enabling you to use the DNeasy
PowerSoil Kit for purification of high-quality DNA.

If automating the DNeasy PowerSoil Kit on the QIAcube, the instrument may process fewer
than 50 samples due to dead volumes, evaporation and additional reagent consumption by
automated pipetting. QIAGEN only guarantees 50 sample preps with manual use of the
DNeasy PowerSoil Kit.

For more information about the automated procedure, see the relevant protocol sheet
available at www.qiagen.com/MyQIAcube. Up-to-date protocol sheets can be downloaded
free of charge, or may be obtained by contacting QIAGEN Technical Services at
support.qiagen.com.

Figure 1. The QIAcube instrument.

8 DNeasy PowerSoil Kit Handbook 05/2017

 Figure 2. DNeasy PowerSoil Kit procedure.

DNeasy PowerSoil Kit Handbook 05/2017 9

Equipment and Reagents to Be Supplied by User
When working with chemicals, always wear a suitable lab coat, disposable gloves and
protective goggles. For more information, consult the appropriate safety data sheets (SDSs)
available from the product supplier.

 Microcentrifuge (10,000 x g)
 Pipettors (50 μl–500 μl)
 Vortex-Genie 2 Vortex
 Vortex Adapter for 24 (1.5-2.0 ml) tubes (cat. no. 13000-V1-24)
 100% ethanol (for the QIAvac 24 Plus Manifold protocol only)
 QIAvac 24 Plus Manifold

Important Notes
 Make sure the 2 ml PowerBead Tubes rotate freely in your centrifuge without rubbing.
 Shake to mix Solution C4 before use.

10 DNeasy PowerSoil Kit Handbook 05/2017

Protocol: Experienced User
Important points before starting
 Perform all centrifugation steps at room temperature (15–25°C).
 If Solution C1 has precipitated, heat at 60°C until precipitate dissolves.
 2 ml Collection Tubes are provided.

Procedure

1. Add 0.25 g of soil sample to the PowerBead Tube provided. Gently vortex to mix.
2. Add 60 μl of Solution C1 and invert several times or vortex briefly.
Note: Solution C1 may be added to the PowerBead tube before adding soil sample
3. Secure PowerBead Tubes horizontally using a Vortex Adapter for 24 (1.5–2.0 ml) tubes
(cat. no. 13000-V1-24).
4. Vortex at maximum speed for 10 min.
Note: If using the 24-place Vortex Adapter for more than 12 preps, increase the vortex
time by 5–10 min.
5. Centrifuge tubes at 10,000 x g for 30 s.
6. Transfer the supernatant to a clean 2 ml Collection Tube.
Note: Expect between 400–500 μl of supernatant. Supernatant may still contain some
soil particles.
7. Add 250 μl of Solution C2 and vortex for 5 s. Incubate at 2–8°C for 5 min.
Note: You can skip the 5 min incubation. However, if you have already validated the
DNeasy PowerSoil extractions with this incubation we recommend you retain the step.
8. Centrifuge the tubes for 1 min at 10,000 x g.
9. Avoiding the pellet, transfer up to 600 μl of supernatant to a clean 2 ml Collection Tube.
10. Add 200 μl of Solution C3 and vortex briefly. Incubate at 2–8°C for 5 min.

DNeasy PowerSoil Kit Handbook 05/2017 11

 Note: You can skip the 5 min incubation. However, if you have already validated the
PowerSoil extractions with this incubation we recommend you retain the step.
11. Centrifuge the tubes for 1 min at 10,000 x g.
12. Avoiding the pellet, transfer up to 750 μl of supernatant to a clean 2 ml Collection Tube.
13. Shake to mix Solution C4 and add 1200 μl to the supernatant. Vortex for 5 s.
14. Load 675 μl onto an MB Spin Column and centrifuge at 10,000 x g for 1 min. Discard
flow-through.
15. Repeat step 14 twice, until all of the sample has been processed.
16. Add 500 μl of Solution C5. Centrifuge for 30 s at 10,000 x g.
17. Discard the flow-through. Centrifuge again for 1 min at 10,000 x g.
18. Carefully place the MB Spin Column into a clean 2 ml Collection Tube. Avoid splashing
any Solution C5 onto the column.
19. Add 100 μl of Solution C6 to the center of the white filter membrane. Alternatively, you
can use sterile DNA-free PCR-grade water for this step (cat. no. 17000-10).
20. Centrifuge at room temperature for 30 s at 10,000 x g. Discard the MB Spin Column.
The DNA is now ready for downstream applications.
Note: We recommend storing DNA frozen (–20°C to –80°C) as Solution C6 does not
contain EDTA. To concentrate DNA, see the Troubleshooting Guide.

12 DNeasy PowerSoil Kit Handbook 05/2017

Protocol: Detailed
Notes before starting
 Perform all centrifugation steps at room temperature (15–25°C).
 If Solution C1 has precipitated, heat at 60°C until precipitate dissolves.
 2 ml Collection Tubes are provided.

Procedure

1. Add 0.25 g of soil sample to the PowerBead Tube provided. Gently vortex to mix.
Note: After your sample has been loaded into the PowerBead Tube, the next step is a
homogenization and lysis procedure. The PowerBead Tube contains a buffer that will (a)
help disperse the soil particles, (b) begin to dissolve humic acids and (c) protect nucleic
acids from degradation. Gentle vortexing mixes the components in the PowerBead Tube
and begins to disperse the sample in the buffer.
2. If Solution C1 has precipitated, heat at 60°C until precipitate dissolves. Add 60 μl of
Solution C1 to sample and invert several times or vortex briefly.
Note: Solution C1 may be added to the PowerBead tube before adding soil sample.
Solution C1 contains SDS and other disruption agents required for complete cell lysis. In
addition to aiding cell lysis, SDS is an anionic detergent that breaks down fatty acids
and lipids associated with the cell membrane of several organisms. If it gets cold, it will
form a white precipitate in the bottle. Heating to 60°C will dissolve the SDS but will not
harm it or the other disruption agents. Solution C1 can be used while it is still warm.

3. Secure PowerBead Tubes horizontally using a Vortex Adapter for 24 (1.5–2.0 ml) tubes
(cat. no. 13000-V1-24).
4. Vortex at maximum speed for 10 min.
Note: If using the 24-place Vortex Adapter for more than 12 preps, increase the vortex
time by 5–10 min. Vortexing is critical for complete homogenization and cell lysis. Cells
are lysed by a combination of chemical agents from steps 1–4 and mechanical shaking

DNeasy PowerSoil Kit Handbook 05/2017 13

 introduced at this step. By randomly shaking the beads in the presence of disruption
agents, collision of the beads with microbial cells will cause the cells to break open. Use
of the Vortex Adapter will maximize homogenization, which can lead to higher DNA
yields. Avoid using tape, which can become loose and result in reduced homogenization
efficiency, inconsistent results and reduced yields.

5. Centrifuge tubes at 10,000 x g for 30 s.

6. Transfer the supernatant to a clean 2 ml Collection Tube.
Note: Expect between 400–500 μl of supernatant. Supernatant may still contain some
soil particles.

7. Add 250 μl of Solution C2 and vortex for 5 s. Incubate at 2–8°C for 5 min.
Note: You can skip the 5 min incubation. However, if you have already validated the
DNeasy PowerSoil extractions with this incubation we recommend you retain the step.
Solution C2 is patented Inhibitor Removal Technology (IRT). It contains a reagent that can
precipitate non-DNA organic and inorganic material including humic substances, cell
debris and proteins. It is important to remove contaminating organic and inorganic
matter that may reduce DNA purity and inhibit downstream DNA applications.

8. Centrifuge the tubes at 10,000 x g for 1 min.

9. Avoiding the pellet, transfer up to 600 μl of supernatant to a clean 2 ml Collection Tube.
Note: The pellet at this point contains non-DNA organic and inorganic material including
humic acid, cell debris and proteins. For best DNA yields and quality, avoid transferring
any of the pellet.
10. Add 200 μl of Solution C3 and vortex briefly. Incubate at 2–8°C for 5 min.
Note: You can skip the 5 min incubation. However, if you have already validated the
PowerSoil extractions with this incubation we recommend you retain the step.
Solution C3 is patented Inhibitor Removal Technology (IRT) and is a second reagent to
precipitate additional non-DNA organic and inorganic material including humic acid,
cell debris and proteins. It is important to remove contaminating organic and inorganic
matter that may reduce DNA purity and inhibit downstream DNA applications.

11. Centrifuge the tubes at 10,000 x g for 1 min.

14 DNeasy PowerSoil Kit Handbook 05/2017

12. Avoiding the pellet, transfer up to 750 μl of supernatant to a clean 2 ml Collection Tube.
Note: The pellet at this point contains non-DNA organic and inorganic material
including humic acid, cell debris and proteins. For best DNA yields and quality, avoid
transferring any of the pellet.
13. Shake to mix Solution C4 and add 1200 μl to the supernatant. Vortex for 5 s.
Note: Solution C4 is a high-concentration salt solution. Since DNA binds tightly to silica
at high salt concentrations, this will adjust the DNA solution salt concentrations to allow
binding of DNA, but not non-DNA organic and inorganic material that may still be
present at low levels, to the MB Spin Columns.
14. Load 675 μl onto an MB Spin Column and centrifuge at 10,000 x g for 1 min. Discard
flow-through.
Note: DNA is selectively bound to the silica membrane in the MB Spin Column device in
the high salt solution. Contaminants pass through the filter membrane, leaving only DNA
bound to the membrane.
15. Repeat step 14 twice, until all of the sample has been processed.
16. Add 500 μl of Solution C5. Centrifuge for 30 s at 10,000 x g.
Note: Solution C5 is an ethanol based wash solution used to further clean the DNA that
is bound to the silica filter membrane in the MB Spin Column. This wash solution
removes residual salt, humic acid, and other contaminants while allowing the DNA to
stay bound to the silica membrane.

17. Discard the-flow through. Centrifuge again for 1 min at 10,000 x g.

Note: This flow-through fraction is non-DNA organic and inorganic waste removed from
the silica MB Spin Column membrane by the ethanol wash solution. The second spin
removes residual Solution C5 (ethanol wash solution). It is critical to remove all traces of
wash solution because the ethanol in Solution C5 can interfere with many downstream
DNA applications such as PCR, restriction digests and gel electrophoresis.
18. Carefully place the MB Spin Column into a clean 2 ml Collection Tube. Avoid splashing
any Solution C5 onto the column.

DNeasy PowerSoil Kit Handbook 05/2017 15

19. Add 100 μl of Solution C6 to the center of the white filter membrane. Alternatively, you
can use sterile DNA-free PCR-grade water for this step (cat. no. 17000-10).
Note: Placing the Solution C6 (sterile elution buffer) in the center of the small white
membrane will make sure the entire membrane is wet. This will result in a more efficient
and complete release of the DNA from the silica MB Spin Column membrane. As
Solution C6 passes through the silica membrane, DNA that was bound in the presence
of high salt is selectively released by Solution C6 (10 mM Tris), which lacks salt.
20. Centrifuge at room temperature for 30 s at 10,000 x g. Discard the MB Spin Column.
The DNA is now ready for downstream applications.
Note: We recommend storing DNA frozen (–20°C to –80°C) as Solution C6 does not
contain EDTA. To concentrate DNA, see the Troubleshooting Guide.

16 DNeasy PowerSoil Kit Handbook 05/2017

Protocol: QIAvac 24 Plus Vacuum Manifold
Notes before starting

 For each sample lysate, use one MB Spin Column. Keep the MB Spin Column in the
attached 2 ml Collection Tube and continue using the Collection Tube as an MB Spin
Column holder until needed for the vacuum manifold protocol.
 Label each Collection Tube top and MB Spin Column to maintain sample identity. If the
MB Spin Column becomes clogged during the vacuum procedure, switch to the
centrifugation protocol.
 100% ethanol will be needed for step 8 of this protocol.

Procedure
1. Connect the QIAvac 24 Plus to the vacuum source using the QIAvac Connecting System
(for more details, refer to the QIAvac 24 Plus Handbook, Appendix A, page 16).
2. Insert a VacValve into each Luer slot of the QIAvac 24 Plus that is to be used. Close
unused Luer slots with Luer plugs or close the inserted VacValve.
3. Insert a VacConnector into each VacValve. Perform this step directly before starting the
purification to avoid exposure of VacConnectors to potential contaminants in the air.
4. Place an MB Spin Column into each VacConnector on the manifold.
5. Transfer 650 µl of prepared sample lysate (from step 13 of the centrifugation protocol) to
an MB Spin Column.
6. Turn on the vacuum source and open the VacValve of the port. Hold the tube in place
when opening the VacValve to keep the MB Spin Column steady. Allow the lysate to
pass through the MB Spin Column completely.
7. After the lysate has passed through the column completely, load again with 650 µl of
lysate. Continue until all the lysate has been loaded onto the MB Spin Column. Close the
VacValve of that port.

DNeasy PowerSoil Kit Handbook 05/2017 17

 Note: If the MB Spin Columns are filtering slowly, close the ports to samples that have
completed filtering to increase the pressure to the other columns.
8. Add 800 µl of 100% ethanol to completely fill the MB Spin Column. Open the VacValve
while holding the column steady. Allow the ethanol to pass through the column
completely. Close the VacValve.
9. Add 500 µl of Solution C5 to each MB Spin Column. Open the VacValve and apply a
vacuum until Solution C5 has passed through the MB Spin Column completely. Continue
to pull a vacuum for another minute to dry the membrane. Close each port.
10. Turn off the vacuum source and open an unused port to vent the manifold. If all 24 ports
are in use, break the vacuum at the source. Make certain that all vacuum pressure is
released before performing the next step. It is important to turn off the vacuum at the
source to prevent backflow into the columns.
11. Remove the MB Spin Column and place in the original labeled 2 ml Collection Tube.
Place into the centrifuge and spin at 13,000 x g for 2 min to completely dry the
membrane.
12. Transfer the MB Spin Column into a new 2 ml Collection Tube and add 100 µl of
Solution C6 to the center of the white filter membrane. Alternatively, sterile DNA-free
PCR-grade water (cat. no. 17000-10) may be used.
13. Centrifuge at 13,000 x g for 1 min at room temperature.
14. Discard the MB Spin Column. The DNA is now ready for downstream applications.
Note: We recommend storing DNA frozen (–20°C to –80°C) as Solution C6 does not
contain EDTA. To concentrate DNA, see the Troubleshooting Guide.

18 DNeasy PowerSoil Kit Handbook 05/2017

Troubleshooting Guide
This troubleshooting guide may be helpful in solving any problems that may arise. For more
information, see also the Frequently Asked Questions page at our Technical Support Center:
www.qiagen.com/FAQ/FAQList.aspx. The scientists in QIAGEN Technical Services are
always happy to answer any questions you may have about either the information and/or
protocols in this handbook or sample and assay technologies (for contact information, visit
www.qiagen.com).

Comments and suggestions

Soil processing

a) Amount of soil to process The QIAGEN DNeasy PowerSoil Kit is designed to process 0.25 grams of
soil. For inquiries regarding the use of larger sample amounts, please contact
Technical Support for suggestions.

b) Soil sample is high in Remove contents from PowerBead Tube (beads and solution) and transfer into
water content another sterile microcentrifuge tube (not provided). Add soil sample to
PowerBead Tube and centrifuge at room temperature for 30 seconds at
10,000 x g. Remove as much liquid as possible with a pipet tip. Add beads
and bead solution back to PowerBead Tube, gently vortex to mix and resume
protocol from Step 2.

DNA

a) DNA does not amplify Make sure to check DNA yields by gel electrophoresis or spectrophotometer
reading. An excess amount of DNA will inhibit a PCR reaction.
Diluting the template DNA should not be necessary with DNA isolated using
the QIAGEN PowerSoil DNA Kit; however, it should still be attempted.
If DNA will still not amplify after trying the steps above, then PCR optimization
(changing reaction conditions and primer choice) may be needed.

b) Eluted DNA is brown If you observe coloration in your samples, please contact Technical Support
for suggestions.

c) Concentrating eluted The final volume of eluted DNA will be 100 μl. The DNA may be
DNA concentrated by adding 4 μl of 5 M NaCl and inverting 3–5 times to mix.
Next, add 200 μl of 100% cold ethanol and invert 3–5 times to mix.
Centrifuge at 10,000 x g for 5 minutes at room temperature. Decant all
liquid. Remove residual ethanol in a speed vac, a dessicator or air dry.
Resuspend precipitated DNA in sterile water or sterile 10 mM Tris.

DNeasy PowerSoil Kit Handbook 05/2017 19

 Comments and suggestions

d) DNA floats out of a well This usually occurs because residual Solution C5 remains in the final sample.
when loading a gel Prevent this by being careful in step 19 and not transferring liquid onto the
bottom of the spin filter basket. Ethanol precipitation (described in
“Concentrating eluted DNA”) is the best way to remove residual Solution C5.

e) Storing DNA DNA is eluted in Solution C6 (10 mM Tris) and must be stored at –20°C to
–80°C to prevent degradation. DNA can be eluted in TE without loss, but the
EDTA may inhibit downstream reactions such as PCR and automated
sequencing. DNA may also be eluted with sterile DNA-free PCR grade water
(cat. no. 17000-10).

Alternative lysis methods

a) Cells are difficult to lyse After adding Solution C1, incubate at 70°C for10 minutes. Resume protocol
from step 3.

b) Reduction of shearing of After adding Solution C1, vortex 3–4 seconds, then heat to 70°C for 5
DNA minutes. Vortex 3–4 seconds. Heat another 5 minutes. Vortex 3–4 seconds.
This alternative procedure will reduce shearing but may also reduce yield.

20 DNeasy PowerSoil Kit Handbook 05/2017

Ordering Information

Product Contents Cat. no.

DNeasy PowerSoil Kit (50) For 50 preps: Isolate microbial

genomic DNA from all soil types 12888-50

DNeasy PowerSoil Kit (100) For 100 preps: Isolate microbial

genomic DNA from all soil types 12888-100

DNeasy PowerSoil HTP 96 Kit For 4 x 96 preps: High-throughput

(384) isolation of DNA from soil samples in 12955-4
less than one day

DNeasy PowerMax Soil Kit (10) For 10 preps: Isolate microbial DNA
from large quantities of soil; great for 12988-10
samples with low microbial load

DNeasy PowerLyzer PowerSoil For 50 preps: Isolate DNA from tough

Kit (50) soil microbes; optimized for use with 12855-50
bead-based homogenizers

DNeasy PowerLyzer PowerSoil For 100 preps: Isolate DNA from

Kit (100) tough soil microbes; optimized for use 12855-100
with bead-based homogenizers

RNeasy PowerSoil Total RNA Kit For 25 preps: Isolate high-quality total
12866-25
(25) RNA from all soil types

MagAttract® PowerSoil DNA KF For 384 preps: Hands-free isolation

Kit (384) of DNA from soil using automated
27000-4-KF
processing and liquid handling
systems

MagAttract PowerSoil DNA KF For 384 preps: Hands-free isolation

Kit (384) of DNA from soil using automated
27000-4-KF
processing and liquid handling
systems

DNeasy PowerSoil Kit Handbook 05/2017 21

Product Contents Cat. no.

Ceramic Bead Tubes, 1.4 mm Ready-to-use bead tubes for rapid

(50) and reliable biological sample lysis
13113-50
from a wide variety of starting
materials

Glass Bead Tubes, 0.5 mm (50) Ready-to-use bead tubes for rapid
and reliable biological sample lysis
13116-50
from a wide variety of starting
materials

Glass Bead Tubes, 0.1 mm (50) Ready-to-use bead tubes for rapid
and reliable biological sample lysis
13118-50
from a wide variety of starting
materials

QIAvac 24 Plus Manifold Vacuum Manifold for processing

1–24 spin columns; includes QIAvac
19413
24 Plus Vacuum Manifold, Luer Plugs,
and Quick Couplings

For up-to-date licensing information and product-specific disclaimers, see the respective
QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are
available at www.qiagen.com or can be requested from QIAGEN Technical Services or your
local distributor.

22 DNeasy PowerSoil Kit Handbook 05/2017

Trademarks: QIAGEN®, Sample to Insight®, DNeasy®, PowerSoil®, PowerLyzer®, MagAttract®, QIAvac®, QIAcube® (QIAGEN Group), Vortex-Genie (Scientific
Industries). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.

Limited License Agreement for DNeasy PowerSoil Kit

Use of this product signifies the agreement of any purchaser or user of the product to the following terms:
1. The product may be used solely in accordance with the protocols provided with the product and this handbook and for use with components contained in the kit
only. QIAGEN grants no license under any of its intellectual property to use or incorporate the enclosed components of this kit with any components not included
within this kit except as described in the protocols provided with the product, this handbook, and additional protocols available at www.qiagen.com. Some of
these additional protocols have been provided by QIAGEN users for QIAGEN users. These protocols have not been thoroughly tested or optimized by QIAGEN.
QIAGEN neither guarantees them nor warrants that they do not infringe the rights of third-parties.
2. Other than expressly stated licenses, QIAGEN makes no warranty that this kit and/or its use(s) do not infringe the rights of third-parties.
3. This kit and its components are licensed for one-time use and may not be reused, refurbished, or resold.
4. QIAGEN specifically disclaims any other licenses, expressed or implied other than those expressly stated.
5. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN may
enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action
to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.
For updated license terms, see www.qiagen.com.

HB-2257-001 © 2017 QIAGEN, all rights reserved.

DNeasy PowerSoil Kit Handbook 05/2017 23

Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com

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1104560 HB-2257-001 05/2017 DNeasy PowerSoil Kit Handbook 05/2017