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    Ospe Answers Biochem

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    Ram Pranav Sai
    1. The document provides information on various biochemical tests performed at different stations including normal ranges for glucose, lipids, electrolytes, enzymes, and others. 2. Chemical and enzymatic methods for estimating blood glucose are described along with their advantages and disadvantages. Sodium fluoride is mentioned as a preservative. 3. Ranges provided for total cholesterol, HDL, LDL and methods for their estimation including ultracentrifugation and gel electrophoresis.

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    © All Rights Reserved
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    Ospe Answers Biochem

    Uploaded by

    Ram Pranav Sai
    194 views6 pages
    1. The document provides information on various biochemical tests performed at different stations including normal ranges for glucose, lipids, electrolytes, enzymes, and others. 2. Chemical and enzymatic methods for estimating blood glucose are described along with their advantages and disadvantages. Sodium fluoride is mentioned as a preservative. 3. Ranges provided for total cholesterol, HDL, LDL and methods for their estimation including ultracentrifugation and gel electrophoresis.

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    1. The document provides information on various biochemical tests performed at different stations including normal ranges for glucose, lipids, electrolytes, enzymes, and others. 2. Chemical and enzymatic methods for estimating blood glucose are described along with their advantages and disadvantages. Sodium fluoride is mentioned as a preservative. 3. Ranges provided for total cholesterol, HDL, LDL and methods for their estimation including ultracentrifugation and gel electrophoresis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    194 views6 pages

    Ospe Answers Biochem

    Uploaded by

    Ram Pranav Sai
    1. The document provides information on various biochemical tests performed at different stations including normal ranges for glucose, lipids, electrolytes, enzymes, and others. 2. Chemical and enzymatic methods for estimating blood glucose are described along with their advantages and disadvantages. Sodium fluoride is mentioned as a preservative. 3. Ranges provided for total cholesterol, HDL, LDL and methods for their estimation including ultracentrifugation and gel electrophoresis.

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    Biochemidhy: OSPE

    Staten
    Nomal fashing blood
    glucoce level: 7o -I10 maldL
    Posk prandtal plasna alueose level:
    2. Chamiaal mehods A O-loluidline method
    b. Folin- Wu's metod

    ymatie melhods :
    a Hexokinase method
    bGlucose oriclase- leroxtdase Mehod

    Chemieal Method : No Specific, tima consurnina


    500
    knzymalie Meltboo : lt shouts lineany or cTo L
    Glucose Concernt akon

    4 Sodtun ourmde acs a an


    mki-8'ycolyie agevt and

    preven aueoyetbnhbng enzyme Enolase, So


    that &a gucos conent Yemauns unhanged for 2-3 dags.

    Stalion 2
    Total cholesnol: 15D-200 mal4
    Taly edas 4-165 mgldl
    HDL- choleshol 20-6o mg
    LDL- cholectaol60- 150 mg
    . Olkacenmfugaten, Coleumn ehom alogphy, Gel Hechophoesie.
    pd all the conanlsaliod
    Post andeal
    ashmaon f
    3 a blood ha al lke LDL Value,
    o lids tn
    Station 3

    lastma blood sugar, Randup Blood Sugar


    HbAle Glyeosyated H.

    HbAIC

    3.
    anage: Gives fasler vesull, capillany blood ean be collecled
    Disacvantage: 1t aivec slightly hgher result than actual.

    alueoce Benediol's Test


    Kelone Bodes-RoItols tect

    ahion 5
    lbumin, Globulin, Hibzinoqen, Paotnombin
    2. Mullkple Myeloma
    3.
    Nephooke ncwone: and nlobulin albumt, prol.ai
    Chvonie echion: m a r albumi
    alobuls

    0lobalins .
    Station
    V t D defeioney
    popathpoidisho, gpepaahyzvidisn
    Fanconi's Sndome
    Hgpohos phalëm1ie Rickeß
    2 Seum Ino ganie phosphous. 2:5 4:9 MEq]L.
    3. Painciple iske and subavvou method.
    In this melld Seun proleans are precaptatad tichlovoacetie
    acid. The ofein -Ree flhte reafe i
    is
    olybdic aid
    1eaganto fon phosphormobedaTa. 1 is reducad b-amim
    2naph t- sulfonte acid (AnsA) to pm molybedenum blue,
    inens fWhoa colouy is a
    measure
    oinovanic phoephate
    S4ahien 1
    .Cavdlae 'hoponins 18-24 h
    Crearine Kinasa : 1- 24 hs
    Laetate Dehydoganate : 24-4
    hs
    2 goalebi

    SHahon 8,o (Communiceatsn SlD

    Staien 9 ldenficakion Spotor


    a Colbtimeler
    b.
    Prorineiple based on Bear- Lamberte law. Panuplef photomeb)
    Estmalion of bleod alucoe level : Glucose oncdase peoridase
    mehod
    Eshimaon blood Urea: Dam meo d
    of
    Low Sensitivi
    Colourless Compownde Canot be analyad.
    Neds elaivelygher amoumb f sample

    . Lic te deqree t which a efaove medium retes


    hansmited a of a
    Itesprelaten slalen (
    Seam Total B1kaabio o-2 13 aL
    2 Seum Unc Aetd 2.6 7 2
    mg jal
    3. Seum Tal oolestiol- 20-200 mad
    4 Seu lanine lzntamnase 5-45 0L
    5: Seao Sodium 135 145 mea|L

    ocedure Statuin (2

    Benxidine Tes est for Blood


    Take 2-3 dhopt f benzdne solo in tst
    Add 2-3 chops of c g e n peroide soln
    Add 12 dops of i s miature *o 2ml unne

    blue colour i oed Wich s


    Cbsevaluon: f a or
    qreen
    minuS ad changes t
    stable only for afew f blood i
    it indioates the Pre
    sence

    bown
    he aven Sample

    hocedure Staton )

    Tes for Caleun


    ake 3 mlf unne sample
    Add Inl of % aoce atid
    Add 3ml f poltasiun oxaale Sp\uiuor.
    t indians t
    nEpreratoin: fsel whu Ppt is med,
    preSenee oftalcuin nhich is Pecpuad as

    Calcuin oxalata.
    Yooblem Solving Statdn
    Cone f blood glucose
    OD of tesk -odofLlank Cone o Std o0
    oD f st- OD blank . of est
    0-21- 0.00
    02 0 00 0.01
    0 : 2 2 x 100 2 8 5 x toD mg|at

    28:5 maa

    2.
    Cone of Saum total poeans
    OD of test - oD of b lank Conc-of std* dotad vol o tewm
    000
    OD of St- oD of blank vo: of Sample
    taken

    0.21-0 00 x 6* 10D
    0-98- D.00 lo00

    021» 6
    5.195 la
    0-28

    Crical Case Stalioo

    Paobable cngonosis RIeKETS.

    Ezlanaioh
    Btochermlcal Iveshgakoh Bona di'sovder due to deseelive mineralicaon

    'Sow caleuin and inoganle phocphate ie decvnased


    Sawo altalne pho4phata le is inetoased
    .
    Caleiol decvased.
    Is

    Cause Bone dtsordar due


    deseot in cal uuo and phexphowe
    melaloltm, Secondy vtamin D dafcien
    Sueckon 11
    Reduaing Cubsuncas BenedioBs Test
    Kefone Bocies- Rolerak Test
    Ble salls- Hay Test
    Proteins-Heat Coagulalion est

    ueshon 16
    Van- Gierkek Diseacealuse- 6- phozphalz
    maltate.
    2.
    ompes DiceaseLuco sewnal
    3. Mc Ale's Dicease- Musele phoaphoylasa
    4 Hers Dicease - Liver phosphoylase

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