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    Activity 7 Inoculation of Microorganisms From The Body

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    Samantha Grace
    This document provides instructions for a laboratory activity to culture bacteria from different sites on the human body. The objectives are to culture bacteria, detect microorganisms, and demonstrate aseptic technique. Students are assigned body sites from which to obtain inoculum using sterile cotton swabs and streak the samples on prepared agar plates. The plates are incubated and later examined to identify and label the microorganisms that have grown from each site.

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    Activity 7 Inoculation of Microorganisms From The Body

    Uploaded by

    Samantha Grace
    14 views3 pages
    This document provides instructions for a laboratory activity to culture bacteria from different sites on the human body. The objectives are to culture bacteria, detect microorganisms, and demonstrate aseptic technique. Students are assigned body sites from which to obtain inoculum using sterile cotton swabs and streak the samples on prepared agar plates. The plates are incubated and later examined to identify and label the microorganisms that have grown from each site.

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    This document provides instructions for a laboratory activity to culture bacteria from different sites on the human body. The objectives are to culture bacteria, detect microorganisms, and demonstrate aseptic technique. Students are assigned body sites from which to obtain inoculum using sterile cotton swabs and streak the samples on prepared agar plates. The plates are incubated and later examined to identify and label the microorganisms that have grown from each site.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    14 views3 pages

    Activity 7 Inoculation of Microorganisms From The Body

    Uploaded by

    Samantha Grace
    This document provides instructions for a laboratory activity to culture bacteria from different sites on the human body. The objectives are to culture bacteria, detect microorganisms, and demonstrate aseptic technique. Students are assigned body sites from which to obtain inoculum using sterile cotton swabs and streak the samples on prepared agar plates. The plates are incubated and later examined to identify and label the microorganisms that have grown from each site.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    SCHOOL OF HEALTH AND NATURAL SCIENCES

    LABORATORY ACTIVITY NO. 7


    INOCULATION OF MICROORGANISMS FROM THE BODY

    Name/s: ________________________________________ Date: ______________


    Course and Year: ______________________________ Code: _____________

    INTRODUCTION
    Animal and human diseases may be classified, according to the causative agent, as bacterial
    diseases, fungal diseases, viral diseases, parasitic diseases, hereditary diseases, and diseases caused by
    environmental factors. Frequently, diseases may be brought on by a multitude of causes. A relatively
    mild viral infection, for example, if favored by hereditary susceptibility, may then weaken the body’s
    resistance to bacterial invasion.
    In this activity, you will culture bacteria from different sources in your body. Remember that
    bacteria are ubiquitous, so, it is expected that you will also collect them from vital parts of the human
    body.

    OBJECTIVES
    1. To culture bacteria from different sites of inoculation.
    2. To be able to detect the presence of microorganisms in the human body.
    3. To demonstrate the aseptic technique in the study of bacteria.

    MATERIALS AND EQUIPMENT


    Prepared Agar Plates Used paper
    Sterile cotton swab Tissue Paper
    Incubator

    PROCEDURE
    1. Take out from the refrigerator the culture media that you have prepared in the previous
    activity.
    2. Remove the excess water that may have accumulated on the surface of the agar media and wipe
    dry the Petri dishes. In doing this procedure, try not to open the Petri dishes so as not to expose
    the media (opening of the Petri dishes should be done only at the designated area of exposure).
    3. Label the Petri dishes that you shall use for inoculation as well as the Petri dishes to be used for
    exposure.
    4. Your instructor will assign from which particular sites of inoculation and areas of exposure are
    you going to obtain your inoculum.

    Inoculation
    1. The following parts of the body are fertile sources of inoculum.

    in-between toes anus Throat


    Inguinal vagina in-between-fingers
    Underarm navel Urine
    2. Moisten the cotton swab and smudge on the desired part of the body where you shall obtain
    your inoculum (or as assigned by your instructor). Streak smudged the swab onto the surface of
    the agar medium.
    3. Do not forget to label your streak lines with the source of the inoculum.

    Page 1 of 3
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    4. Wrap all the inoculated Petri dishes with used paper and incubate at 37OC to be used for the
    next activity.

    ILLUSTRATION
    1. Illustrate microorganisms that have grown in your Petri dishes. Label the sources.

    Page 2 of 3
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    DISCUSSION

    1. From your set-up, which part of your body is the most thickly populated by bacteria?
    _____________________________________________________________________________________
    _____________________________________________________________________________________

    2. Based on your answer in item number 1, why do you think so?


    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________

    3. What kind of bacteria, according to morphological features, is the most abundant in the human
    body?
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    __________________________________________________________________________________

    Do you think there is a part of the body that cannot be inhabited by bacteria? What made you
    say so?
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________

    GENERALIZATION
    ___________________________________________________________________________________________
    ___________________________________________________________________________________________
    ___________________________________________________________________________________________
    ___________________________________________________________________________________________
    __________________________________________________________________________________________

    NAME CONTRIBUTION/PARTICIPATION PERSONAL PROTECTIVE


    EQUIPMENT

    Date Submitted: _______________________ Score: __________________


    Instructor: ____________________________

    Page 3 of 3
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