ISO Common Name Chemical Name: Metazachlor 411
ISO Common Name Chemical Name: Metazachlor 411
ISO Common Name Chemical Name: Metazachlor 411
METAZACHLOR
411
CH3
O
Cl
N CH2
CH3 CH2
N
N
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METAZACHLOR 411
METAZACHLOR TECHNICAL
*
411/TC/M/-
3 Metazachlor
OUTLINE OF METHOD The active ingredient is chromatographed by HPLC
on a reversed phase column (C18, methanol/water) and quantitatively determined
by UV detection with external calibration.
REAGENTS
Methanol HPLC grade, e. g. methanol LiChrosolv® from Merck
Water HPLC grade
Mobile phase methanol/water 3 + 2 (v/v)
Metazachlor standard of known purity
Calibration solution. Weigh (to the nearest 0.1 mg) about 270 mg of metazachlor
standard (s mg) into a 50 ml volumetric flask and dissolve quantitatively in 6 ml
methanol. Make up to volume with mobile phase. Adjust the amount of
metazachlor (s mg) such that the peak height is not more than 80 % of full
scale. In case of doubt, check the linearity of the detector. Prepare two
calibration solutions of similar concentrations. Do not use the same
calibration solutions for periods longer than one week.
APPARATUS
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METAZACHLOR 411
PROCEDURE
(a) Preparation of sample solution. Weigh (to the nearest 0.1 mg) into a 50 ml
volumetric flask enough sample (w mg) to contain about 270 mg pure metazachlor.
Dissolve in 6 ml methanol and make up to volume with mobile phase. The
concentration of metazachlor should be similar to its concentration in the
calibration solutions. Prepare three solutions for each sample.
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METAZACHLOR 411
(d) Calculation
Calibration factor:
Hs
f
s
where:
Hs = area of the metazachlor peak in the calibration solution
s = mass of metazachlor standard taken (mg)
Hw P
Metazachlor content
f w
where:
Hw = area of the metazachlor peak in the sample solution
P = purity of metazachlor standard (g/kg)
w = mass of the sample taken (mg)
The content of metazachlor is the mean value of the results of the three sample
solutions.
2 Identity tests
2.1 Infrared. Weigh about 0.1 g of sample into a 50 ml centrifuge tube, add 50 ml
of acetone, stir for 1 h and centrifugate for 20 min at about 3000 min -1. Transfer
the supernatant, clear solution into a 100 ml round-bottomed flask and evaporate
to dryness under reduced pressure. Dry the residue for 2 h at 60 °C in vacuo.
Proceed as described for 411/TC/M/2.1.
2.2 HPLC. As for 411/TC/M/2.2
*
CIPAC method 1992. Prepared by the German Committee (DAPA), Chairman: Dr W Dobrat.
Based on a method supplied by BASF (FRG)
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METAZACHLOR 411
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