1.4. Microbiology QP

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WJEC (Eduqas) Biology A-level

Topic 1.4: Microbiology


Questions by Topic
1.

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3. (a) Define the following terms with reference to bacterial growth.

[3]

(i) obligate aerobe

(ii) obligate anaerobe

(iii) facultative anaerobe

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(b) Describe and explain the appearance of Gram positive and Gram negative bacteria following Gram

staining.

[3]

(c) Most pathogens in humans are Gram-positive organisms. Six Gram-positive genera are typically

pathogenic in humans. Two of these, Streptococcus and Staphylococcus, are cocci. The remaining
organisms are bacilli.

What three dimensional shape would the cocci and bacilli be?

[1]

cocci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

...........

bacilli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

..........

4. 1 m3 of air was filtered from two different environments.

The microorganisms collected were grown, using aseptic technique, on four different types of agar plates
(A, B, C and D) at the same temperature and for the same length of time.

The number of colonies grown from each sample is shown.

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(a) What conclusion can you draw from the graph above about the numbers of microbes in the two air

samples?

[1]

(b) The four agar types have resulted in different colony numbers because they contain different nutrients.
State four ways that the agar types could differ in composition.

[2]

i. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

..........

ii. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

..........

iii. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

..........

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iv. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

..........

5. Complete the sentences below by giving the correct term in each case. [4]

(a) A clone arising from a single bacterium on an agar plate is called a. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

(b) A viable count is a count of . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . bacterial cells.

(c) The type of bacterium that lacks lipopolysaccharides in its cell walls is a

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . bacteria.

(d) A corkscrew or helical shaped bacterium is known as a . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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