Determination of Isoniazid and Pyridoxine Hydrochloride Levels in Tablets With Ultraviolet Spectrophotometry by Successive Ratio Derivative
Determination of Isoniazid and Pyridoxine Hydrochloride Levels in Tablets With Ultraviolet Spectrophotometry by Successive Ratio Derivative
Determination of Isoniazid and Pyridoxine Hydrochloride Levels in Tablets With Ultraviolet Spectrophotometry by Successive Ratio Derivative
95-101
Corresponding Author:
Rida Evalina Tarigan,
Institut Kesehatan Helvetia Medan
Email: [email protected]
1. INTRODUCTION
In general, Anti Tuberculosis Drugs (OAT) used are isoniazid, rifampin, ethambutol,
pyrazinamide and streptomycin. This group of drugs is called the primary drug used as
standard therapy. To obtain the effectiveness of treatment and prevent the emergence of
resistance, it is necessary to avoid the use of monotherapy, by combining several types of
drugs in sufficient quantities and in appropriate doses according to the treatment category.
However, without quality assurance, the bioavailability of the drug will be reduced, the risk of
toxicity or under- dose and over-dose can facilitate the development of drug resistance (1).
2. RESEARCH METHODE
This research was conducted at the USU Faculty of Pharmacy Research Laboratory in
October-November 2021. The tools used in this research are complete ultraviolet-visible
spectrophotometry (Shimadzu 1800) with a personal computer (PC) equipped with UV probe
2.42 software, MS Excel, sonicator (Branson), analytical balance (Sartorius), measuring flask
(Pyrex). , measuring cup (Pyrex), beaker glass (Pyrex), maat pipette (kimax), mortar and
pestle. The materials used in this study were tablets containing isoniazid 400 mg and
pyridoxine hydrochloride 10 mg Pehadoxin Forte® (PT. Pharpros), isoniazid (BPFI),
pyridoxine hydrochloride (BPFI), 0.1 N HCl and aquadest. The samples of this study were
tablets containing isoniazid 400 mg and pyridoxine hydrochloride 10 mg Pehadoxin Forte®
(PT. Pharpros) which were obtained from a pharmacy in Medan city. The data obtained is in
the form of absorbance spectrum measurement results which are calculated with the help of
UV Probe and Ms Excel software.
Absorption Spectrum
0,5165
0,4000
0,2000
0,0000
200,00 250,00 300,00 350,00 400,00
nm.
0,8794
0,8000
0,6000
0,4000
0,2000
0,0000
200,00 250,00 300,00 350,00 400,00
nm.
0,8794
0,8000
0,6000
0,4000
0,2000
0,0000
200,00 250,00 300,00 350,00 400,00
nm.
226,0103
0,0000
-500,0000
-1000,0000
-1120,8019
200,00 250,00 300,00 350,00 400,00
nm.
2713,5306
2000,0000
1000,0000
0,0000
-439,8367
200,00 250,00 300,00 350,00 400,00
nm.
1,0000
0,0000
-1,0000
-1,2573
200,00 250,00 300,00 350,00 400,00
nm.
Method Validation
Accuracy, Precision (RSD), Linearity, Limit of Detection (LOD) and Limit of Quantity (LOQ)
Table 1. Accuracy, Precision (RSD), Linearity, Limit of Detection (LOD) and Limit of
Quantity (LOQ)
4. CONCLUSION
Based on the research conducted, it can be concluded that the determination of the levels of
isoniazid and pyridoxine hydrochloride in tablet preparations using the ultraviolet
spectrophotometric method using successive ratio derivatives meets the requirements of the
Indonesian Pharmacopoeia Edition VI..
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