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ISBN: 978-0-12-800167-7
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CONTRIBUTORS
Michael A. Ackley 27 Warren D. Hirst 43

John J. Baldwin 3 John Hynes Jr. 117
Amelia Black 437 Pravin S. Iyer 209
Michel Bouvier 285 Ravi Jalluri 135
Eli Breuer 269 Sylvie E. Kandel 347
Joanne Bronson 437 Kareem Khoury 167
Matthew F. Brown 399 Bethany L. Kormos 43
Sean P. Brown 77 Jed N. Lampe 347
David Burns 135 Jonathan Lawrence 249
Allorie T. Caldwell 87 Christopher Liu 249
Monalisa Chatterji 209 Adam R. Looker 383
Murali Dhar 437 Gabriel Martinez-Botella 27
Kirk Dinehart 383 John P. Miller 317
Alexander D€
omling 167 Jeffrey N. Miner 151
James J. Doherty 27 Peter Mueller 383
Bruce Ellsworth 437 Michael J. Munchhof 399
Natalia Estrada-Ortiz 167 Satheesh K. Nair 117
Heather Finlay 101 Shridhar Narayanan 209
Mark E. Flanagan 399 Constantinos Neochoritis 167
Pontus Forsell 59 Pascal Neuville 285
Stephen Frye 301 Gunnar Nordvall 59
Paul Galatsis 43 Gregory T. Notte 417
Jean-Luc Girardet 151 Gregory R. Ott 189
William J. Greenlee 11 Reuven Reich 269
Peter D.J. Grootenhuis 383 J. Robert Merritt 437
Vincent Guerlavais 331 Jonathan R.A. Roffey 189
Sabine Hadida 383 Francesco G. Salituro 27
Yong He 249 Tomi K. Sawyer 331
Jaclyn Henderson 43 Stephan Schann 285
xv
xvi Contributors
Peggy Scherle 135 Frederick Van Goor 383

Niu Shin 135 Xiaodong Wang 301
Pravin S. Shirude 209 William N. Washburn 363
Michael J. Sofia 221 E. Blake Watkins 87
Chakrapani Subramanyam 399 Larry C. Wienkers 347
Claudiu T. Supuran 269 Wenqing Yao 135
Bradley D. Tait 317 Ning Yin 249
Joshua P. Taygerly 77
PREFACE
The Annual Reports in Medicinal Chemistry is dedicated to furthering genuine

interest in learning, chronicling, and sharing information about the discov-
ery of compounds and the methods that lead to new therapeutic advances.
ARMC’s tradition is to provide disease-based reviews and highlight emerg-
ing technologies of interest to medicinal chemists.
A distinguishing feature of ARMC is its knowledgeable section editors in
the field who evaluate invited reviews for scientific rigor. The current
volume contains 27 chapters in 8 sections. The first five sections have a
therapeutic focus. Their topics include CNS diseases (edited by Albert J.
Robichaud); cardiovascular and metabolic diseases (edited by Robert L.
Dow); inflammatory, pulmonary, and gastrointestinal diseases (edited by
David S. Weinstein); oncology (edited by Shelli R. McAlpine); and infec-
tious diseases (edited by William J. Watkins). Sections VI and VII review
important topics in biology (edited by John A. Lowe) and new technologies
for drug optimization (edited by Peter R. Bernstein). The last section deals
with case histories and drugs approved by the FDA in the previous year
(edited by Joanne Bronson).
The format for Volume 49 follows our previous issue. We continue with
the personal essays, which express the personal stories and scientific careers
of “drug hunters”; the volume opens with essays by John Baldwin and
William Greenlee Jr. In all, there are 14 therapeutic focused chapters as well
as 6 chapters that review important topics in biology and new technologies
for drug optimization. The last section includes case histories for the three
recently approved drugs, dapagliflozin, ivacaftor, and tofacitinib.
This would not have been possible without our panel of section editors,
to whom I am indeed very grateful. I would like to thank the authors of this
volume for their hard work, patience, dedication, and scholarship in the
lengthy process of writing, editing, and making last-minute edits and
revisions to their contributions. Further, I extend my sincere thanks to
the following reviewers who have provided independent edits to the man-
uscripts: George Chang, Margaret Chu-Moyer, Kevin Currie, Dario Doller,
Jennifer Davoren, Carolyn Dzierba, Bruce Ellsworth, Gary Flynn, Allen
Hopper, Doug Johnson, Jeff Kropff, Mary Mader, Amanda Mikels-Vigdal,
Bernard Murray, Anandan Palani, Greg Roth, Joachim Rudolf, Subas
Sakya, Adam Schrier, Steven Spregel, Kirk Stevens, James Taylor, Lorin
Thompson, and Jeff Zablocki.
xvii
xviii Preface
Finally, I would like to thank John Primeau, who was the section editor
for the infectious diseases section for Volumes 43 (2008) through 48 (2013),
for his dedication to identifying the most relevant topics of interest to the
medicinal chemists and his editorial expertise.
I hope the material provided in this volume will serve as a precious
resource on important aspects of medicinal chemistry and, in this way, main-
tain the tradition of excellence that ARMC has brought to us for more than
four decades. I am excited about our new initiatives; I look forward to hear-
ing from you about them and welcome your suggestions for future content.
MANOJ C. DESAI, Ph.D.
Gilead Sciences, Inc.
Foster City, CA
CHAPTER ONE
A Personal Essay: My Experiences

in the Pharmaceutical Industry
John J. Baldwin
Gwynedd Valley, PA, USA
Content
References 8
I am a chemist, a medicinal chemist, born in Wilmington, Delaware, the

cradle of the U.S. chemical industry, the home of DuPont and its post-
monopoly offspring, Hercules and Atlas. Wilmington borders on three riv-
ers, the Delaware, the Brandywine, and the Christiana. The fourth
connecting side was dominated by the three research centers of DuPont
and its spin-offs.
Like most budding scientists, I had the obligatory chemistry sets. My
dream was to work someday in those beautiful research centers in the
Wilmington suburbs, surrounded by parks and golf courses. (Of course there
were the other dreams, maybe being a Bishop or riding on horseback into
the sunset with imaginary cowboy friends, but that’s another story.)
When choosing science as a career, it is important to develop a strong
foundation in mathematics, languages, biology, chemistry, and physics. It
is also important to immerse yourself in a motivating and competitive envi-
ronment. A strong work ethic is critical. When I attended the University of
Delaware as a chemistry major, I carried a full schedule each year while
working 24 h a week in the analytical lab at Halby Chemical. My interest
in biology was evident in my course selection: biology, physiology, and
psychology. My thesis, under John Wriston, focused on the possible fate
of one-carbon fragments produced during metabolism. At the University
of Delaware, I felt that I had prepared myself to dig deeper into the biological
sciences in graduate school. Work hard; study harder.
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ISSN 0065-7743 All rights reserved.
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4 John J. Baldwin
For graduate school, I chose the University of Minnesota and was for-
tunate to receive a teaching fellowship. I majored in Organic Chemistry
and minored in Biochemistry, which was structured within the Medical
School. My mentor in synthetic chemistry was Lee Smith, a member of
the National Academy of Science and an expert in quinone chemistry, espe-
cially as it related to vitamin E and coenzyme Q. My minor in Biochemistry
required 2 years of heavy course work. My mentor here was Paul Boyer, the
Nobel Prize winner. Working with both of these men was a great experi-
ence. My research in synthetic organic chemistry and the courses within the
Medical School prepared me well for a future in drug discovery. Work hard;
study harder.
After saying farewell to graduate school in 1960, I joined the Medicinal
Chemistry Department of Merck and Company’s West Point, Pennsylvania,
laboratory. The company, then known as Merck Sharp & Dohme, had
formed through a merger following the discovery at Sharp & Dohme of
hydrochlorothiazide, a game changing medication for the treatment of
hypertension and, in my thinking, the beginning of modern hypothesis-
driven drug discovery.
The Merck laboratory in Rahway, New Jersey, focused on antibiotic
chemistry, which had evolved from the penicillin program; steroids, which
grew out of cortisone synthesis; and vitamins, stemming from their fine
chemicals background. The West Point laboratory was devoted to cardio-
vascular and CNS diseases, antisecretory/antiulcer agents, atherosclerotic
disease, and antiviral agents.
I became involved in many of the West Point programs, including loop
diuretics, xanthine oxidase inhibitors, beta adrenergic blockers, vasodilators,
dopamine agents for Parkinson’s disease, antivirals, and carbonic anhydrase
inhibitors for glaucoma. Learning pharmacology was never ending in the
Merck environment. Work hard; study harder.
The areas that were especially attractive to me were the mechanistic
approach to drug discovery and understanding the biochemistry of disease,
using computationally intensive predictive methods and X-ray crystallogra-
phy of ligand–protein complexes. From this work came Edecrin, Crixivan,
Trusopt, Cosopt, and the antiulcer agent famotidine (Pepcid), which
I identified in the patent literature and championed through the Merck sys-
tem. The work, that led to the first topically available carbonic anhydrase
inhibitor, dorzolamide (Trusopt), has been described in terms of its design,
computational understanding, conformational analysis, and X-ray crystallo-
graphic details of the enzyme/ligand structure.1 This integration of
A Personal Essay: My Experiences in the Pharmaceutical Industry 5
disciplines established a powerful approach to drug design that I repeated in

the discovery of the HIV protease inhibitors at Merck and the renin inhib-
itors at Vitae.
A compound that created excitement within research but failed to reach
the market was the topically penetrating, direct-acting dopamine agonist for
Parkinson’s disease. The compound was administered via patch and effec-
tively controlled symptoms. However, as sometimes happens, the Research
Division could not convince the Clinical/Marketing Organization to con-
tinue with clinical trials.
In 1993, Merck began to prepare for the twenty-first century and the
predicted patent cliff which lay ahead. One step was to decrease staff through
a retirement incentive plan. Cut-backs not only pose difficult decisions for
management but impose difficult decisions on the people affected as well.
Such actions by Merck and other companies marked the disappearance of
the lifelong commitment of an employee to a single company and the belief
that the commitment of the company to this contract would also be hon-
ored. A new era in the company/employee relationship had begun. This
new reality became even more apparent over the past decade, and it must
be considered by professionals and students alike as they make choices about
employment. I adjusted to this apparent evolutionary change by deciding
not only to take Merck’s offer but to start a new company, Pharmacopeia,
which was based on cutting-edge technology not being practiced in Big
Pharma. Jack Chabala, also from Merck, and Larry Bock of Avalon Ventures
joined me in launching the new company around encoded combinatorial
chemistry and high-throughput screening. This technology played a key
role in exploiting the genomic revolution and the target-specific approach
to drug discovery.
Pharmacopeia perfected the synthesis of encoded solid-phase combina-
tional libraries, which allowed the preparation and screening of large com-
pound collections across numerous in vitro assays. These screening
collections, built on a common theme, would be followed by smaller librar-
ies focused on the bioactive lead.
Within a few years, the approach was adopted by all pharmaceutical
companies, and the ability to generate large screening collections became
a technology that was required within every drug discovery organization.
The only advantage that Pharmacopeia had in selling what had become a
commodity was price and its experience in drug discovery. One obvious
solution to the price issue was to move compound production to a
lower-cost environment. After first looking into a joint venture possibility
6 John J. Baldwin
in China and finding no interest at Pharmacopeia, I, along with one of our

founding scientists, Ge Li, and three others decided to finance a new com-
pany, WuXi Pharma Tech. This Contract Research Organization grew rap-
idly and is the largest company in China servicing the research needs of the
U.S. and European pharmaceutical industries.
With WuXi Pharma Tech successfully listed on the New York Stock
Exchange, it was time to start something new. That new endeavor was Con-
current Pharma, later Vitae Pharma, which was based on computational
methods from Eugene Shakhnovich’s laboratory at Harvard. The method
defines in detail a ligand–protein complex that was constructed in silico, frag-
ment by fragment, along the surface of the target. The computational exer-
cise was followed by chemical synthesis of the predicted ligand and
appropriate in vitro testing. The predicted complex then could be verified
by X-ray crystallography. We found a problem that was ideally suited to test
the technology among the aspartic acid proteases, renin and beta-secretase.
The approach produced novel, nonpeptidic, and bioavailable inhibitors of
both enzymes. A similar approach was used to find inhibitors of
11-beta-hydroxysteroid dehydrogenase, a flexible enzyme that offered an
additional challenge. This de novo design strategy holds great promise for
the future, especially for the virtual company that lacks the internal architec-
ture of Big Pharma.
During this evolutionary period of Vitae Pharma, the drug-discovery
capability in China continued to develop such that the time seemed right
for a new company not only capable of research support but also of drug
discovery, development, toxicology, registration, and human clinical trials,
each component present in a virtual organization. So, Bob Nelson of Arch
Ventures and I decided to start a new company, Hua Medicine. This China-
centric company will move its first drug candidate into human Phase
I clinical trials this year, 2013.
Looking back to the start of the biotech era, it is clear that start-up com-
panies continue to be a high-risk exercise. Whether the company is large or
small, it still takes years and over a billion dollars to discover and develop a
New Chemical Entity (NCE). This forces the small biotech firm to be in a
constant search for financing and for a viable exit strategy.
Recent years have not been easy ones, especially for the major pharma-
ceutical companies, which faced a slowing rate of discovery, major products
going “off patent” into the generic class, higher R&D costs, and longer
approval times. To combat this, a range of new survival strategies has been
developed and adopted,2,3 including a series of mega-mergers with the
resulting cut-back in employment. Since NCEs are the main factor in raising
valuation, it is unlikely that the merger strategy will increase value to the
pharmaceutical industry. Mergers like Merck/Schering-Plough, Pfizer/
Wyeth, Bayer/Schering, and Sanofi/Aventis will, at best, produce only
short-term stability. History teaches us that such mergers simply do not
increase the productivity on which valuation is based. Such retrenchment
stimulated an outsourcing trend that has accelerated over the past decade.
The layoffs from mergers and outsourcing reached 130,000 between
2005 and 2008, with the total number now well over 300,000 people.4,5
Few companies were spared: the top 10 pharma layoffs in 2011, in rank
order, were Merck, Pfizer, Novartis, Abbott, Astra Zeneca, Teva, Sanofi,
Johnson & Johnson, Eisai, and Bayer.6 WuXi Pharma Tech, one of the first
to recognize the growth in outsourcing and profit from it, went public on
the New York Stock Exchange in 2000 and now has over 6000 employees.
Along with the outsourcing of science, there has been growth in alliance
among large pharmaceutical companies themselves as well as with academic
institutions, all with the idea of sharing both cost and risk.7–9 With the West-
ern markets mature, Big Pharma has turned to the evolving economies as
part of its growth strategy. However, emerging markets have shown resis-
tance to patented medication and a greater interest in improving health
through the availability of low-cost generic drugs.10,11
To overcome the generic competition, Big Pharma has turned to
“branded generics.” Even with cost only somewhat above the local generic
equivalent, pricing remains a problem for Western companies.12 The cost of
patented Western drugs in these economies is being countered by price con-
trols and forced licensing.13 Considering both of these marketing issues, fast-
growing evolving economies may not be the answer for the problems of
today’s pharmaceutical companies.
With the uptick in new FDA approvals in the past 2 years (2011–2012), it
has been suggested that the worst may be over for the productivity decline
we have seen in the pharmaceutical industry.14 However, considering that
programs for these approvals were initiated in the late 1990s to early 2000s, it
is reasonable to predict that the decrease in productivity expected from the
cut-backs will not be felt for at least another 10–12 years.
Things have changed in the pharmaceutical industry since I first walked
the halls of Merck. These changes and the resulting strategies discussed here
have had a negative impact for those who have worked as scientists on drug
R&D and even those who hope to join the exciting adventure of drug dis-
covery. The sky is red, but it is difficult to tell whether it is the sunrise or the
8 John J. Baldwin
sunset. Looking to the future, it is certain that there are many road blocks
along the road to recovery. For synthetic organic chemists, the path ahead
is not bright but should stabilize as the cost advantage of outsourcing to India
and China decreases. In the USA, there will be fewer opportunities for
chemists than there will be for biologically trained scientists as the revolution
in biotechnology continues. Many of these opportunities will be in industry-
sponsored academic laboratories, although the productivity of this indus-
trial/academic strategy may decline over the next decade as Big Pharma
increasingly depends on licensing new products from non-U.S. biotechs.
Government laboratories, such as the National Institutes of Health and
the National Cancer Institute, are unlikely to replace the industrial discovery
machine that gave the world new drugs. Therefore, society will become
increasingly dependent on generic medications as new drugs constitute a
smaller percentage of total sales. With government budget pressures, generic
drugs will become commodities with low profit margins. This may create
the kind of shortages seen recently due to deteriorating production facilities
and poor quality control, which leads to forced closings and recalls by gov-
ernment agencies.
The introduction of biosimilars will be slower than expected, and their
price will not decrease as dramatically as in the case of small-molecule drugs.
The high prices for niche drugs will come under increasing pressure, as dem-
onstrated by the Sloan-Kettering Cancer Center’s experience with the anti-
cancer drug Zaltrap. Similar forced pricing and licensing decisions by India
and other countries on expensive, patented drugs will be a growing problem
for the Western pharmaceutical industry. Companies will begin to rethink
whether it is worth pursuing low-volume drugs where high prices are
needed to recover cost. Similarly, society must decide whether it is willing
to pay high prices for drugs that extend the life of a very ill person for just a
short period of time. These questions pose ethical issues that are difficult
to solve.
For young scientists who received their first chemistry set under the
Christmas tree, all these issues are only challenges for tomorrow. To play
in the sandbox of drug discovery, you must be driven. Work hard;
study harder.
REFERENCES
1. Greer, J.; Erickson, J. W.; Baldwin, J. J.; Varney, M. D. J. Med. Chem. 1994, 37,
1035–1054.
2. Baldwin, J. J. Future Med. Chem. 2011, 3(15), 1873–1876.
3. Edwards, J. Leaner Isn’t Meaner for 9 Big Pharma Firms That Tried to Cut Their Way to
Greatness. www.industry.bnet.com/Pharma/10008581/leaner-isnt-meaner-for-9-big-
Pharma.
4. Johnson, L. Merck Plans More Job Cuts. The Reporter, Jul 30, 2011.
5. Novartis Cuts 1,400 US Jobs. The Wall Street Journal, Nov 30, 2011.
6. McBridem, R.; Hallmer, M. http://www.fiercepharma.com/special-reports/top-10-
pharma-layoffs-2011.
7. DeArment, A. http://www.drugstorenews.com/article/astrazeneca-broad-institute-
partner-antibiotic-antiviral-drugs.
8. PharmaLive. http://pharmalive.com/news/Print.cmf?articleid¼877597.
9. Timmerman, L. http://www.xconomy.com/national/2011/06/20/pfizers-idea-to-fix-
the-drug-development.
10. Silverman, E. http://www.pharmalot.com/2012/10/India-pushes-to-end-sale-of-
branded-drugs/.
11. Staton, T. http://www.fiercepharma.com/node/91359/print.
12. Herper, M. http://www.forbes.com/sites/matthewherper/2012/07/12/The-global-
drug-market.
13. Silverman, E. http://www.pharmabt.com/2012/10/australia-plans-to-preview.
14. Christel, M. D. Tide Turns for Innovation. R&D Directions, Jan/Feb 10–11, 2012.
CHAPTER TWO
Adventures in Medicinal
Chemistry: A Career in Drug
Discovery
William J. Greenlee
MedChem Discovery Consulting, LLC, Teaneck, New Jersey, USA
Contents
Acknowledgments 21
References 21
I was born into a family of chemists. My father, uncle, and grandfather were
all chemists. As a result, my brother Mark and I were exposed to laboratories
and the basic concepts of chemistry from an early age. We got used to having
our father enliven family outings by throwing a lump of sodium into the
nearest body of water and having complex chemistry discussions with our
grandfather. I got my own start in the laboratory during high school, syn-
thesizing acetylenes using reactions in liquid ammonia, running Grignard
reactions, and monitoring large-scale distillations for my father’s new catalog
company “Chemical Samples Company.” During that time, my intention
was to study medicine, but I was drawn into the world of organic chemistry
around me. I went on to major in chemistry at Ohio State University, doing
undergraduate research with Paul Gassman. I completed my Ph.D. with
Robert B. Woodward at Harvard University, and after postdoctoral work
with Gilbert Stork at Columbia University, I joined Merck Research Lab-
oratories in Rahway, New Jersey in 1977. It was not a surprise that Mark also
got his Ph.D. in chemistry and has had a long and successful career as a
medicinal chemist.
One thing that drew me to organic and (especially) medicinal chemistry
was the concept of creating a new chemical compound that had never
existed before. The idea that I could do that, and that the compound might
be useful in treating disease was a powerful motivation. Later, when I was
working at the bench, one of my favorite occurrences was spotting the first
#
Annual Reports in Medicinal Chemistry, Volume 49 2014 Elsevier Inc. 11
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12 William J. Greenlee
crystals growing in a solution of a new compound. Creating a new com-

pound and watching how it forms crystals for the first time was a near-
religious experience for me. Although I have been out of the laboratory
for many years, I still dream about being back there doing experiments
and experiencing the excitement of drug discovery.
I began my career at Merck in the New Lead Discovery group led by
Art Patchett, who had also received his Ph.D. at Harvard University with
R.B. Woodward. The goal of my first project at Merck was to synthesize
halovinyl amino acids as irreversible inhibitors for DOPA decarboxylase
(for Parkinson’s disease (PD)) and alanine racemase (as potential antibacterial
agents).1 This type of inhibitor was referred to as a “suicide substrate,” one
that became reactive only after turnover by the enzyme target (also referred
to as a “mechanism-based enzyme inactivator”). I was also able to work out a
more general synthesis of β,γ-unsaturated amino acids using the Strecker
reaction.2 Drugs with an irreversible mode of action fell out of favor for
many years, but irreversible inhibitors of protein kinases have become pop-
ular, and there are now many examples of marketed drugs that form covalent
bonds with the target protein.
H
X
H X = -F
Fluorovinylglycine
H2N CO2H
X = -Cl
Chlorovinylglycine
By the time I arrived at Merck from Columbia, Art had already started a
program to identify inhibitors of angiotensin-converting enzyme (ACE), to
follow up on earlier discoveries made at Squibb (now Bristol-Myers Squibb).
At the time, the antihypertensive drug Aldomet™ was a successful product for
Merck, but would be off patent in a few years, and a drug that would treat
hypertension with fewer side effects was a major goal. I was asked to join
the effort and was privileged to be part of the team, along with Matt Wyvratt,
Eugene Thorsett, and others, who discovered enalapril and lisinopril, both of
which became important drugs for Merck.3 For many of us, this was our first
experience working with amino acids and small peptides, and doing reactions
and workups in aqueous solution. The ACE inhibitor program was successful
even though the X-ray crystal structure of ACE was not available. Amazingly,
the details of the structure and the fact that ACE has two nonidentical active
sites were not known until many years later.
Adventures in Medicinal Chemistry 13
After ACE inhibitors were moving ahead in development, Merck and

other companies shifted their attention to renin inhibitors, which block
an earlier step in the renin–angiotensin pathway, and were thought to have
potential advantages. Merck had started working on renin even before the
ACE inhibitor program began, but the initial screen using porcine renin had
not yielded viable hits. Like most other companies, Merck pursued a pep-
tidomimetic approach to renin inhibitors, and the team at the Merck West
Point site, led by Dan Veber and Joshua Boger, used the X-ray crystal struc-
ture of a fungal aspartic protease (Rhizopus pepsin) as a model enzyme.
NH2
EtO O HO O
CH3
N N
N N
H O OH H O OH
O O
Enalapril Lisinopril
(Vasotecä) (Prinivilä)
Their elegant work led to a potent and selective hexapeptide inhibitor, but
oral bioavailability was low, and the program was eventually put on hold.
A few years later, we began a new effort in Rahway, now guided by a pub-
lished X-ray structure of human renin. We made significant progress in
reducing the peptide nature of our inhibitors and identifying potent macro-
cyclic inhibitors,4 but we could not achieve the desired profile for an orally
bioavailable inhibitor. At one point, there were over 20 companies with
renin inhibitor programs, but none of the resulting candidates progressed
beyond early clinical trials. It became clear that identifying peptidomimetics
with drug-like properties would be a difficult challenge. It was over 20 years
later (2007) when the first renin inhibitor, aliskiren, was approved for treat-
ment of hypertension.5
It was therefore very exciting when DuPont Pharmaceuticals disclosed
the first potent nonpeptide angiotensin II receptor antagonist and reported
that it had efficacy in a rat model of hypertension. Rather than inhibiting the
formation of angiotensin II, the receptor antagonist losartan blocks the
action of the peptide at its receptor. With encouragement from senior man-
agement, the Merck renin inhibitor program was immediately abandoned,
and all effort shifted to the new target. I soon found myself leading a large
medicinal chemistry effort with an ambitious goal to identify a development
candidate without delay. However, after a few months, Merck announced a
new collaboration with DuPont Pharmaceuticals to develop their candidate

losartan (Cozaar™). Our medicinal chemistry program and the ongoing
program at DuPont Pharmaceuticals led by Ruth Wexler were merged into
a combined effort to identify backup and second-generation antagonists.
This work led to several backup candidates, including 1, MK-996, and
DuP532 that were derived from the losartan chemotype.6,7 It had become
clear that there are two receptors for angiotensin II, AT1 and AT2, and
that losartan blocks only AT1 receptors. A potent AT2 antagonist had been
disclosed by a group at Parke-Davis, but despite numerous studies, the sig-
naling pathways and roles of this receptor were unclear (and still are). By
modifying MK-996 and losartan, the team was able to identify potent dual
AT1/AT2 antagonists such as 2 and XR510, but in the absence of a clear
understanding of the AT2 receptor, none of these were taken forward
into development.8 In the meantime, Cozaar™ was launched as a mon-
otherapy and in combination with hydrochlorothiazide (Hyzaar™), and
both were very successful drugs for treatment of hypertension. Several other
AT1 antagonists (also called angiotensin receptor blockers) based on the
losartan lead were launched by competitors and also became successful
drugs (valsartan, candesartan, telmisartan, irbesartan, eprosartan, azilsartan,
olmesartan).
OH
N N H3C
N N N N
N
N H N N
Cl N H
N nBu
H3C N Et
Losartan (Cozaarä) 1
H3C O
N H Ph
O N H
O N Ph O
N S N O N O
H N S iPn
H3C N O
Et H3C N O
Et
MK-996 2
nPr
O H3C
N (3-Pyr) N H
O
F H O N O
O O N S nBu
O N O O
N S iPn H3C N
Et Et S
N O
nPr
3 iBu
XR510
One day during our backup program, Pete Siegl, who directed the
Merck pharmacology effort at West Point, called to tell me that one of
our “antagonists” had raised blood pressure when dosed to rats. In fact,
we had discovered the first nonpeptide AT1 agonist, 3.9 This compound
was found to be a full agonist of the AT1 receptor, with a much longer dura-
tion of action than angiotensin II, which is rapidly degraded. Although non-
peptide agonists of peptide G-protein-coupled receptors are now common,
at that time 3 was the only such compound identified outside of the opioid
receptor field. We were astounded by how small a modification in structure
was required to shift an antagonist to an agonist.10 Recently, 3 became the
starting point for design of AT2 selective agonists (without effects on blood
pressure) that are being explored for potential utilities.11
Soon after we completed our angiotensin II program, the discovery of
the vasoactive peptide endothelin was reported, and it was demonstrated
to be the most potent vasoconstricting peptide to date. There was hope that
blocking the endothelin receptors ETA and ETB, or preventing biosynthesis
of endothelin by blocking endothelin-converting enzyme, would provide a
novel (and possibly superior) approach to treating hypertension. Working
from screening leads, we were able to identify potent dual ETA/ETB recep-
tor antagonists, including 4.12 A few of our antagonists also had activity as
angiotensin receptor antagonists, and remarkably we discovered compounds
that blocked all four receptors (ETA, ETB, AT1, AT2) with nanomolar affin-
ity.13 While this work did not continue at Merck, others followed up on
similar leads and were able to demonstrate reduction of blood pressure in
hypertensive patients.14 A number of selective endothelin receptor antago-
nists have been developed by others, but (similar to the situation with the
angiotensin II receptors) the ETA receptor appears to be linked to vasocon-
striction, while the roles of the ETB receptor are still unclear. Interestingly,
both ETA selective (ambrisentan) and dual ETA/ETB antagonists (bosentan,
macitentan) are approved for treatment of pulmonary arterial hypertension.
O OH
O O O
O S
N
H
O
O
4
Art Patchett’s interests in medicinal chemistry were very broad, and

working with him provided me and others with broad training in medic-
inal chemistry. I also benefitted greatly from working with other managers
and mentors at Merck, including Burt Christensen, Ralph Hirschmann,
and Tom Salzmann. Although I was mostly involved in cardiovascular
programs, I also had the opportunity to work on medicinal chemistry
programs to discover antibacterial and antifungal agents and several pro-
jects in inflammation. Merck was an outstanding place to work, and
I was privileged to be there during the “most admired” era of a world-class
company.
After nearly two decades at Merck, I accepted a position at Schering-
Plough in Kenilworth, NJ, to lead the Cardiovascular and CNS Medicinal
Chemistry group. I was excited to join the Schering-Plough team and to
work directly with Catherine Strader, who had also recently relocated
from Merck. I had been fascinated by neuroscience for many years, and
the opportunity to contribute in this area was particularly attractive.
I quickly found myself involved in programs for Alzheimer’s disease (mus-
carinic M2 receptor antagonists), PD (adenosine A2A antagonists), and
schizophrenia (dopamine D4 antagonists). Many of our CV/metabolic dis-
eases projects (e.g., NPY5 antagonists, MCHR1 antagonists, MC4 ago-
nists) also involved targets residing in the CNS, so most of the programs
we took on required drug candidates with good brain penetration. This
was a steep learning curve for me, since designing drugs for CNS penetra-
tion adds a new dimension of complexity to lead optimization. I was also
gaining an appreciation for the liabilities of CNS side effects in otherwise
excellent drug candidates.
One of our most challenging CNS programs was to identify a potent
and selective adenosine A2A receptor antagonist for the treatment of PD.
This project was initiated and actively promoted by Ennio Ongini at
Schering-Plough’s Neuroscience Center in Milan, Italy. Ennio and his
group had produced a very convincing set of data in rodent models
of PD for the potent A2A antagonist SCH 58261, which had been
designed and synthesized by his collaborator Professor Pier Baraldi at
the nearby University of Ferrara.15 This antagonist, which was only
modestly selective versus the A1 receptor (10-fold), had extremely
low aqueous solubility and had to be dosed in DMSO by intraperitoneal
injection.
NH2
N N N
N O
N
N
SCH 58261
NH2
N N N
H3C O O N N O
N
N
N
Preladenant
Although improving selectivity was straightforward, increasing solubility

while maintaining robust activity in the in vivo models turned out to be a
major challenge. Many attempts to reduce the complexity of the tricyclic
core and replace the furan ring gave antagonists with high binding potency
(1–5 nM), but led to loss of activity in the primary rat catalepsy model,
despite high free drug levels in the brain. The adenosine antagonist program
was not unique in this respect, and similar experiences in more recent pro-
grams have convinced me that we do not yet fully understand how to design
effective CNS drugs. The CNS multiparameter optimization approach
introduced recently by Pfizer16 may be a step in the right direction. Of sev-
eral thousand antagonists synthesized for the program, preladenant emerged
as our development candidate, and it possessed the properties we believed to
be essential for success.17 Preladenant demonstrated robust activity in rodent
and primate models of PD and had excellent pharmacokinetics and receptor
occupancy in humans. Unfortunately, although it showed promising activ-
ity in Phase 2 studies in PD, preladenant did not demonstrate sufficient effi-
cacy in Phase 3 studies to support continued development.
One of the first projects initiated soon after I joined Schering-Plough was
a program to identify a potent thrombin receptor antagonist as a potential
antiplatelet agent. Activation of the thrombin receptor, also known as
protease-activated receptor-1 (PAR-1), activates platelets and promotes
their aggregation as part of thrombus formation. Unlike most G-protein-
coupled receptors that are activated by an external ligand, PAR-1 receptors
are cleaved at their amino terminus by the enzyme thrombin, creating a new
peptide sequence that functions as a “tethered ligand” to activate the
receptor. We were extremely fortunate that one of the hits to emerge from
high-throughput screening became an excellent lead for the program. The
hit was an analog of the natural product Himbacine, which had been syn-
thesized for another program at Schering-Plough. The chemistry team,
headed by Sam Chackalamannil, brought two development candidates for-
ward, but each of these ran into a toxicity issue. Fortunately, the team was
able to address these issues, and the third candidate, SCH 530348
(vorapaxar), moved into clinical trials for prevention of arterial thrombosis.18
I was excited to have the opportunity to co-chair the Early Development
Team for vorapaxar with Madhu Chintala, Head of Pharmacology for the
preclinical program. After completion of the Phase 3 clinical program for
vorapaxar (which enrolled over 41,000 patients), the NDA was filed in
201319 and was approved by the FDA in May of 2014. Vorapaxar (proposed
trade name Zontivity™) is hoped to provide benefits in secondary preven-
tion of heart attack and stroke.20
O H
H H
N O
O H
O
H3C H H
F
Vorapaxar
(Zontivityä)
Soon after the discovery of beta-amyloid-converting enzyme-1

(BACE-1) in 1999, Schering-Plough initiated a program to discover inhib-
itors of this enzyme which plays a key role in the biosynthesis pathway of
beta-amyloid and formation of the extracellular plaques found in
Alzheimer’s disease. I was highly motivated to be a part of this effort, since
Alzheimer’s disease has affected my family, and I had seen the effects of this
terrible disease. BACE-1 is an exceedingly challenging target, due to its loca-
tion in organelles inside neurons in the brain. BACE-1 is anchored in the
membrane, but its active site faces an aqueous environment that requires
inhibitors with substantial aqueous solubility. At Schering-Plough, we
screened our entire sample collection and drew upon external screening
resources, but were unable to generate leads for our program. Efforts to
work from nonpeptide renin inhibitors in the scientific and patent literature
were also unsuccessful. Reluctantly, we began an effort to design a pep-
tidomimetic BACE-1 inhibitor based on the amyloid precursor protein sub-
strate. Although we were able to identify potent inhibitors with oral
bioavailability using this approach, these inhibitors were P-glycoprotein
(PGP) efflux substrates and showed exceedingly low brain penetration.21
Given these setbacks, it was very exciting when Dan Wyss and Yu-Sen
Wang in our structural chemistry group presented us with fragment hits
that they had identified using HSQC NMR screening. Follow up by
X-ray crystallography provided several confirmed hits, including an iso-
thiourea that bound in the BACE-1 active site, making multiple interactions
with the two active site aspartic acids. The isothiourea was clearly an unde-
sirable group to have in a drug molecule, and a key insight provided by
Zhaoning ( Johnny) Zhu was to replace this basic group with a five-
membered heterocycle (iminohydantoin) in order to make similar interac-
tions. A large team, led by Andy Stamford, worked to optimize the weak
iminohydantoin lead, which later evolved into a six-membered ring series
of iminopyrimidinones.22,23 The BACE-1 program was supported by chem-
ists at Pharmacopeia, and later by chemists at Albany Molecular Research Inc.
As with most CNS programs, achieving potent binding affinity was only part
of the challenge. Although most potent inhibitors in the series reduced beta-
amyloid levels in plasma, reducing them in cerebrospinal fluid (CSF) and
(especially) in cortex was much more difficult. We expected that a balance
of lipophilicity, aqueous solubility, and low PGP efflux would be essential
for good activity in the CNS. However, it also became clear that a high degree
of inhibition of BACE-1 was required for CNS activity. Our failure to
achieve good inhibition of BACE-1 in the cortex became a big concern,
and it was fortunate that we identified an iminopyrimidinone (5) that showed
good activity in both CSF and cortex with an ED50 of 6 mg/kg (po) in rats.24
SCH 900931, later renamed MK-8931, was shown to reduce A-beta-40
levels by over 80% in a Phase 1 rising multiple dose study.25 This inhibitor
is now in Phase 3 clinical studies in Alzheimer’s disease patients. Throughout
the BACE-1 program, the medicinal chemistry team received strong support
from our structural chemistry group led by Corey Strickland, and from other
groups in discovery research. Merck has made a strong commitment to
develop MK-8931 in both mild-to-moderate AD and in patients with mild
cognitive impairment.26 If successful, this inhibitor could make a major con-
tribution to the treatment (and possibly prevention) of this terrible disease.
H H
N H3C NH
H CH3
S N HN N
H
S
O
H
O N
Cl
Cl
Fragment lead 5
After having succeeded in identifying a potent BACE inhibitor for

development, we wondered whether the same iminoheterocycle chemo-
type could be used for inhibition of the related aspartyl protease renin.
We were able to convince senior management at Schering-Plough to give
us 6 months to come up with a renin inhibitor with good oral bioavailability,
a property that was lacking in the successful renin inhibitor aliskiren. Know-
ing the extensive literature on the roles of the renin–angiotensin system in
the brain, we were also interested to see what advantages a brain-penetrant
renin inhibitor might have. By mining Schering-Plough’s BACE-1 inhib-
itor collection and carrying out rapid and creative lead optimization, Brian
McKittrick and Tanweer Khan were able to discover potent and selective
renin inhibitors such as inhibitor 6 (human renin Ki ¼ 0.6 nM).27 Having
struggled with peptidomimetic renin inhibitors during 1980s, it was partic-
ularly gratifying to have this success over 25 years later. We wondered
whether iminoheterocycles might prove to be a general scaffold for aspartyl
protease inhibitors, and interestingly, a series of iminohydantoins have
recently been reported which are potent inhibitors of the aspartyl protease
plasmepsin, with potential for treatment of malaria.28
F
H N
N H
H N O N
O
Renin inhibitor 6
Throughout my career, I have been privileged to work with talented and

dedicated scientists whose dream has been to contribute to the discovery of
new medicines to reduce human suffering. Their commitment and persis-
tence in the face of continual setbacks have been a source of inspiration. I am
grateful for the support and encouragement that I have received from so
many colleagues, and the opportunity to make a difference. There have been
many changes since I joined Merck over 35 years ago. When I began as a
new medicinal chemist there, I had every expectation that I would be at
Merck my entire career, and in the end I moved only a few miles away
to Schering-Plough, and later had the chance to rejoin Merck after the
merger. No chemist hired today can have the expectation of a long, secure
career with a single company. News of layoffs of medicinal chemists has
become so frequent that it is easy to become cynical about the future of
medicinal chemistry as a career path. In spite of the new uncertainty, there
has never been a more exciting time to be involved in drug discovery, and
I believe that new targets and approaches in discovery research offer a bright
future for medicinal chemists.
What guidance can we offer to medicinal chemists in the industry now or
contemplating a career there? Here are my words of advice: (1) Do your
best, stay focused on drug discovery, and work hard to gain a deep and crit-
ical understanding of medicinal chemistry. Expand your reading beyond
chemistry to acquire a basic knowledge of related disciplines such as phar-
macology, drug metabolism, and toxicology. (2) Build your resume and try
to get one publication, presentation, and patent (at least) from each project
you contribute to, so that your contributions can be recognized. Do not put
off writing the paper, even if you are busy with the next project. (3) Find
ways to contribute more to your projects and to medicinal chemistry.
Become an expert on an important topic and write a review. Look for
opportunities to become involved in medicinal chemistry outside your
company, including volunteering in support of the American Chemical
Society. (4) Above all, maintain a sense of urgency in your work, since what
you are doing is important to the lives of patients. Remember that you have
limited time in your career to make a difference, and take advantage of your
opportunities. I wish you success and happiness in your efforts.
ACKNOWLEDGMENTS
I would like to acknowledge the many outstanding colleagues who have contributed so much
to my career in medicinal chemistry. I have mentioned just a few in the text, without the
intention to overlook others. In addition, I would like to acknowledge Ashit Ganguly,
Michael Czarniecki, Duane Burnett, Deen Tulshian, John Clader, Stuart McCombie,
Cecil Pickett, Ismail Kola, Ann Weber, and Malcolm MacCoss.
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ACS Med. Chem. Lett. 2014, 5, 89.
CHAPTER THREE
Natural and Synthetic Neuroactive

Steroid Modulators of GABAA
and NMDA Receptors
Gabriel Martinez-Botella, Michael A. Ackley, Francesco G. Salituro,
James J. Doherty
Sage Therapeutics, Cambridge, Massachusetts, USA
Contents
1. Introduction 27
2. NAS Modulators of the GABAA Receptor 29
2.1 Endogenous NAS Modulators of the GABAA Receptor 31
2.2 Synthetic NAS Modulators of the GABAA Receptor 32
3. NAS Modulators of the NMDA Receptor 36
4. Conclusions 38
References 39
1. INTRODUCTION
Neuroactive steroids (NASs) are a family of steroid-based compounds
of both natural and synthetic origin, which have been shown to impact cen-
tral nervous system (CNS) function through allosteric modulation of the
GABA(γ-aminobutyric acid)A receptor1–4 and the N-methyl-D-aspartic acid
(NMDA) class of glutamate receptors.5 Respectively, these are two of the
major inhibitory and excitatory neurotransmitter receptor families involved
in synaptic transmission in the brain.
Research over recent years has demonstrated that these receptors can
be either positively (PAM) or negatively (NAM) allosterically modulated
by NAS compounds, an action mediated through binding to allosteric sites.6
Evidence suggests that endogenous NASs (Fig. 3.1), such as all-
opregnanolone (1), are capable of modulating GABAA receptors at concen-
trations that are relevant to their endogenous levels.4,9 Additionally, recent
Annual Reports in Medicinal Chemistry, Volume 49 # 2014 Elsevier Inc. 27

http://dx.doi.org/10.1016/B978-0-12-800167-7.00003-1
28 Gabriel Martinez-Botella et al.
Figure 3.1 Biosynthesis of endogenous neuroactive steroids (NASs) of the GABAA and
NMDA receptor.7,8
evidence has shown that cholesterol (2) metabolism in the brain is respon-
sible for the generation of a specific, endogenous NAS modulator of NMDA
receptors (24(S)hydroxycholesterol, 3).10
O OH
H H H
H H H H H H
HO HO HO
H
1 2 3
Due to their potential to modulate the overall excitatory and inhibitory

tone in the nervous system, it is proposed that specific NAS modulators may
be used to treat a broad range of CNS disorders, such as epilepsy, autism,
schizophrenia, depression, and pain, in which the excitatory/inhibitory
homeostasis is perturbed. The focus of this report is to briefly review endog-
enous and synthetic NASs that target GABAA and NMDA receptors.
Modulators of GABAA and NMDA Receptors 29
2. NAS MODULATORS OF THE GABAA RECEPTOR

The GABAA receptor is a pentameric ion channel formed by assem-
bling two alpha subunits (α1–α6), two beta subunits (β1–β3), and one
additional subunit (γ1–γ3, δ, ε, π, or θ).11 The subunit composition deter-
mines the biophysical and pharmacological characteristics of the channel as
well as influencing its location at synaptic or extrasynaptic sites. For exam-
ple, γ subunits have been associated with clustering the receptor at synap-
tic sites and thus mediating fast inhibitory synaptic transmission. On the
other hand, receptors that contain the δ subunit have been associated with
extrasynaptic receptors that mediate tonic inhibitory conductances.12 Fur-
thermore, certain subunit combinations are restricted to specific anatomic
locations affording the opportunity to target individual neuronal circuits.
Gating of the channel allows chloride ions to flow along their electro-
chemical gradient resulting in a modification of the resting membrane
potential and change in the resistance of the cell membrane. Under the
majority of conditions, this results in an inhibitory influence on the cell,
reducing its ability to transduce excitatory inputs into action potentials. As
the major inhibitory neurotransmitter in the nervous system, GABA act-
ing via an array of GABAA receptors can influence a wide range of brain
circuits that are central to a variety of behavioral states, such as anxiety
levels, sleep, and memory. GABAA dysregulation also sits at the center
of a range of neuropsychiatric diseases such as schizophrenia and mood
disorders. Perhaps not surprisingly, given its critical role in the function
of neuronal circuits, the GABAA receptor is the target for numerous clin-
ically prescribed drugs such as benzodiazepines, barbiturates, and certain
anesthetics.
NASs have the potential to differentiate from classical GABAA receptor
modulators in several ways. First, NASs differentiate from benzodiazepines
by targeting different populations of GABAA receptors. NASs have been
suggested to putatively bind to three or four distinct sites on the GABAA
receptor. At low concentrations, NASs bind to a site in the M3/M4
domains of the α subunit,6 leading to allosteric modulation of the
GABA-induced current. This modulation could be in either a positive
(PAM) or a negative (NAM) direction. At high concentrations, direct gat-
ing of GABAA currents may be achieved in the absence of GABA via bind-
ing at the α/β interface near the GABA-binding site.6 At least one or two
alternate sites have also been suggested to be responsible for the inhibitory
30 Gabriel Martinez-Botella et al.
effects of sulfated NASs on GABA currents.13 On the other hand, benzo-

diazepines bind to an allosteric site distinct from the GABA-binding site, at
the interface of the α and γ subunits.14–17 This limits their ability to poten-
tiate synaptic GABA currents to receptor assemblies that contain a γ sub-
unit.17 Conversely, NASs potentiate the GABAA receptor by binding to
residues within the obligatory α subunit and so modulate receptors in a
manner which is agnostic to their subunit composition. In this way, and
unlike benzodiazepines, NASs are capable of targeting extrasynaptic GABA
receptors that include the δ subunit in addition to synaptic γ-containing
receptors. As such, NASs may exhibit a therapeutic advantage over benzo-
diazepines, for example, in indications such as benzodiazepine-resistant
seizures.18
Interestingly, data also suggest that NASs can exert profound effects on
expression levels of GABAA receptors.19–21 For example, in the hippocam-
pus, brief exposure to low concentrations of NASs can enhance tonic cur-
rents while having little effects on phasic synaptic currents even after a
prolonged wash.20,22 These data suggest that NASs could influence the traf-
ficking and surface expression of certain GABAA receptors. Consistent with
this hypothesis, recent data suggest that NASs do indeed enhance trafficking
of GABAA receptor subunits.23 Such an effect would further differentiate
NAS allosteric modulators of the GABAA receptor from benzodiazepines.
Indeed, benzodiazepines typically display tolerance to their effects on
repeated dosing.18
As NASs have complex modulatory actions via allosteric sites on the
receptor complex, this has historically had implications for determining
structure–activity relationships (SARs) using available pharmacological
tools. The ability of NASs to modulate the binding of the picrotoxin site
ligand, t-butylbicyclophosphorothionate (TBPS), has been used to identify
compounds that bind to the GABAA receptor.24 As this represents an allo-
steric interaction between the NAS- and TBPS-binding sites, there is not
necessarily a linear relationship between functional potency of the NAS
and inhibition of TBPS. Additional functional assays can be helpful to fur-
ther characterize compounds that were promising based on the initial data.
In fact, the gold standard for measuring functional activity of ligand-gated
ion channels is using patch clamp electrophysiology and it is only with
the recent advent of automated high-throughput patch clamp systems such
as the QPatch systems (Sophion) and Ionworks Barracuda (Molecular
Devices) that drug discovery has been able to screen in a high-throughput
manner and to fully understand SARs.25
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mature, and the animal is for a time hermaphrodite; later on, however,
when all the spermatozoa are used up, the worm is a female. Beard's
"dwarf males" are therefore merely the young of hermaphrodite forms.
In cysticolous species each cyst usually contains a large female
individual and a small male. In these cases the young one (male)
discharges all its spermatozoa before the ova ripen, so that a period of
immaturity intervenes and a true hermaphrodite condition is omitted;
the animal is at first male, and later female. The Myzostomaria are thus
"protandric hermaphrodites."
The affinity of these animals has been much discussed; they

superficially resemble the Tardigrada in many anatomical features, and
differ greatly from Chaetopoda, but as they possess the characteristic
chaetae or parapodia, and pass through a larval stage[400] similar to
that of the Polychaetes, there is no doubt that they are closely allied to
the group, and indeed may be regarded as degenerate Chaetopods. It
has been suggested that they form a passage group between them and
the Tardigrada; and von Graff forms a group Stelechopoda, to include
the Myzostomaria, the Tardigrada, and the Linguatulida.
OLIGOCHAETA (EARTHWORMS, ETC.) AND
HIRUDINEA (LEECHES)
BY
F. E. BEDDARD, M.A. (Oxon.), F.R.S.

Prosector to the Zoological Society
CHAPTER XIII
OLIGOCHAETA (EARTHWORMS AND THEIR ALLIES)
INTRODUCTION—ANATOMY—REPRODUCTION—BIONOMICS—DISTRIBUTION
—CLASSIFICATION—MICRODRILI AND MEGADRILI
The Oligochaeta form a well-marked branch of that exceedingly large

assortment of animals vaguely spoken of as worms, and embracing a
number of types many of which have no near relationship to each other.
From this great and unnatural group, which has survived as "Vermes"
even in some quite modern text-books, we can separate off those forms
which show a plain segmentation or division of the body internally as
well as externally into a series of more or less similar rings, as a Class
Chaetopoda. This Class, consisting of the Orders mentioned on p. 241,
includes the worms which form the subject of the present chapter—the
Oligochaeta, as they were originally called by Grube, on account of the
fewness of their chaetae as compared with the number possessed by
the majority of the Polychaeta.
Our knowledge of this group, as of so many others, dates from

Aristotle, who called the earthworms the "intestines of the earth." But it
is only very recently that the numerous and remarkable genera of exotic
earthworms have been anatomically investigated; indeed the common
British species was not really well known before the publication of the
memoirs of Lankester[401] and Claparède[402] in 1864 and 1868, in
spite of the elaborate quarto devoted to it by Morren,[403] the botanist,
in 1826. Some of the aquatic species afforded material to Bonnet and
Spallanzani for their experiments upon the powers of regeneration of
the animals when cut into fragments, while the work of O. F. Müller[404]
upon various Naids is a monument of careful anatomical description.
Our knowledge of the aquatic Oligochaeta does not appear to have
advanced so rapidly as has that of the earthworms.
External Characters.—The most salient external characteristic of this

group of worms, which vary from 1 mm. to 2 metres in length, is of
course the segmentation. The entire body is divided into a number of
rings, which are for the most part similar to each other; a fragment of an
earthworm's body could not be accurately replaced unless it had been
cut from the anterior region. There is precisely the same regular
segmentation in the aquatic representatives of the Order. At the anterior
end of the body in the common earthworm (and in nearly all
Oligochaeta) is a small unpaired lobe, which overhangs the mouth, and
is usually termed the prostomium; the mouth itself is surrounded by the
first segment of the body, which never bears any chaetae in any
Oligochaete. The prostomium is occasionally greatly developed, and in
such cases doubtless forms a tactile organ of importance. This is
especially the case with the South American genus Rhinodrilus, where
the lengthy prostomium can be retracted at will. The aquatic Nais
lacustris (= Stylaria proboscidea) has also an exceedingly long
prostomium, which cannot, however, be retracted, though it is
contractile. At a certain distance from the anterior end of the body, fixed
for the species, but varying greatly from genus to genus and from
species to species, is the clitellum. This region of the body (popularly
believed to mark the spot where a worm divided by the gardener's
spade has come together again) is associated with the reproductive
function, and serves to secrete the cocoon in which the creature's eggs
are deposited. It has in the earthworm a thick glandular appearance. A
more minute examination of the worm's body will show the orifices of
the reproductive ducts and of the excretory organs which will be found
described below. In addition to these, all British earthworms and a large
percentage of the tropical forms have a row of pores along the back,
which are between the successive segments in the median line. These
"dorsal pores" open directly into the body-cavity, and are mere
perforations of the body-wall, not tubes lined by a special layer of cells.
Professor Spencer of Melbourne[405] has observed a giant earthworm
(Megascolides australis) of Gippsland which, when held in the hand,
spurts out, to a height of several inches, the fluid of the body-cavity
through its dorsal pores. The burrows, he remarks, are coated over with
the same fluid, which is regarded by him as a lubricant. This, however,
considering that the glandular cells of the epidermis can secrete a
mucous fluid, seems to be an expensive use to which to put the
important fluids of the interior of the body. It is more probable that the
dorsal pores are a means of getting rid of waste products. Lim Boon
Keng[406] suggests that the coelomic fluid possesses a bactericidal
function. The dorsal pores are missing in many earthworms, and
without exception in those Oligochaeta which live in water; but these
latter worms have a pore upon the head, which appears to be wanting
in the earthworms. Dr. Michaelsen has thought that the head-pore
serves to relieve the brain from undue watery pressure—to act, in fact,
as a kind of safety-valve for the liberation of superfluous fluid.
In some foreign worms the pores of the reproductive ducts are

conspicuous external features (Fig. 197); even in our British species the
turgescent male apertures upon the fifteenth segment are sometimes
quite obvious.
Structure of the Body-Wall.—The body-wall consists in all

Oligochaeta of three recognisable sheets of tissue. Outside is the
epidermis, which always consists of a single layer of cells, except in the
clitellar region of earthworms. It is a point of difference between the
aquatic genera and the terrestrial forms that in the former the clitellum
is only one cell thick, while in the higher Oligochaeta it is made up of
more than one layer of cells. The epidermis is ciliated only in the genus
Aeolosoma, and there only on the prostomium. It secretes a thin layer
of chitin, which is defective opposite to the glandular cells, and
becomes therefore perforated by numerous pores. The structure of the
epidermis of Lumbricus has been studied by Cerfontaine, whose recent
account[407] of the same is the fullest and most accurate that exists.
Underneath the epidermis comes a layer of circular muscle-fibres, and
underneath this again a layer of longitudinal muscles. In both layers the
fibres have a softer core, outside which lies the radially striated
muscular substance. The fibres are embedded in a granular matrix. It
used to be considered at one time that such medullated fibres were
distinctive of leeches as opposed to Oligochaeta. Their existence has
been really known in the Oligochaeta since the researches of Ratzel;
but Cerfontaine has fully described them, and emphasised the fact that
the fibres of both circular and longitudinal coats are alike in this respect.
Fig. 187.—Chaetae of Oligochaeta. × 10. (After Michaelsen, Stolc, and

Vejdovsky). 1, 2, Penial chaetae of Acanthodrilus georgianus; 3,
Spirosperma; 4, Ilyodrilus; 5, Lophochaeta; 6, Tubifex; 7, 8, Nais; 9,
Bohemilla. Figs. 3-9 are ordinary chaetae.
Chaetae.—The passive organs of locomotion in these animals are the

chaetae, which are absent in only one family, Discodrilidae, and in one
other genus, Anachaeta. In this latter worm the chaetae are
represented by large glandular cells, which seem to correspond to the
cells from which the chaetae arise in other forms. They are in this case,
as in the others, cells of the epidermis. The chaetae of the Oligochaeta
are not quite so variable in form as in the marine Polychaeta (see Fig.
138, p. 267). Figs. 187 and 188 illustrate some of the principal shapes
which these bristles assume. The most prevalent form is an elongated
S, which has been aptly compared to the mathematical sign ∫. This kind
of chaeta is found in all earthworms, and in not a few aquatic genera
such as the Lumbriculidae. In some of the latter and in the Tubificidae
and Naids there is the same form of chaeta, which is cleft at the free
end, and possibly enables the worm to grasp the leaves of aquatic
plants, and otherwise facilitates progression in a laxer medium than the
stiff soil frequented by the earthworms. Even earthworms, at any rate
the genus Pontoscolex, have chaetae of this kind; some of the aquatic
Oligochaeta have elongated and hair-like bristles, such as that of
Tubifex.
Fig. 188.—Chaetae. × 10. 1, Onychochaeta; 2, Pontoscolex; 3,

Trichochaeta; 3b, the same, more highly magnified.
In the Tubificid Lophochaeta (Fig. 187, 5) the chaetae are ornamented

on both sides with delicate processes, which give them the appearance
of Crustacean hairs. Among earthworms the simple S-like form is
sometimes complicated by the development of sinuous ridges upon the
distal end. No doubt these bristles enable their possessor to get a
firmer grip of adjacent objects; they are very commonly found, in the
family Geoscolicidae, upon the segments of the clitellum, and permit of
a firmer union during sexual congress. In no Oligochaeta are the
chaetae borne upon parapodia, as is the case with the Polychaeta; but
in many of the aquatic forms there are a considerable number to each
bundle. In earthworms the number of chaetae varies greatly. The
common earthworms of this country, belonging to the genera
Lumbricus, Allolobophora, and Allurus, have only eight chaetae upon
each segment of the body, and these are then, as a rule, arranged in
pairs or rather couples, two of each on each side of the body. The
genus Perichaeta and some of its allies have a much larger number of
chaetae to each segment, disposed in a continuous row round the
middle of the segment. The intermediate condition is to be seen in the
genus Deinodrilus, where there are twelve in each segment, and in
certain members of the genus Megascolex, where there are eight in
each segment in the anterior region of the body, the number increasing
in the posterior segments. The four bundles of chaetae in the Naids and
Tubificids have been likened to the notopodia and neuropodia of the
Polychaetes; but it does not seem certain that this comparison is
justifiable. It was at one time thought that the continuous circle of
chaetae of the Perichaetidae was the primitive condition; but Professor
Bourne has lately found that in Perichaeta the young embryos have not
got this continuous circle; it is only acquired later.
Branchiae.—The Oligochaeta were called by Cuvier the "Annélides

abranches sétigères." But the epithet "abranches" is now known to be
inaccurate. In fact it really was so when Cuvier wrote; for naturalists
were at that time well acquainted, chiefly through the elaborate work of
O. F. Müller, with the little fresh-water Naid Dero, the posterior extremity
of which is provided with a varying number of branchial processes.
These are furnished with looped blood-vessels and are covered
externally by cilia, so that the water containing oxygen is constantly
renovated. The second instance of a gilled Oligochaete was discovered
in the very same family. Professor Bourne[408] of Madras found in
"tanks" a Naid which he named Chaetobranchus, in which the head
segments, to the number of fifty or so, are provided with long ciliated
processes, which as a rule enclose the dorsal chaetae of their
segments, and in addition a capillary loop. Curiously enough, this very
same worm made its appearance in the Victoria regia tank at the
Botanical Gardens in the Regent's Park, whither it had in all probability
been accidentally imported. Two members of the family Tubificidae
were the next examples of gilled Oligochaeta made known to science;
one of these, Branchiura sowerbyi,[409] appeared also in the Botanical
Gardens, so that its native home is unknown. It differs from
Chaetobranchus in that the gills are at the posterior end of the body,
and are contractile; during the life of the worm they are in continual
motion. A species of the South American genus Hesperodrilus,[410] H.
branchiatus, is also gilled, and, so far as can be made out from a spirit-
preserved specimen, the gills are precisely of the same pattern and
contractility as those of its ally Branchiura. Possibly Branchiura ought to
be included in the same genus with Hesperodrilus. A worm which was
originally described by Grube as Alma nilotica, should really have been
placed before the three last-mentioned instances; but as this worm was
only known from a fragment, and as the description was not by any
means full, it was not thoroughly believed in; it was surmised that it
might be a member of some marine genus, perhaps of the Capitellidae.
Oddly enough, the same worm was independently described by a
different name, Digitibranchus niloticus, a few years later by Levinsen.
Quite recently Michaelsen has found by a reference to the original
types that this worm is really gilled, and that it is specifically identical
with a worm which had been given a totally different name, viz.
Siphonogaster. The fact that the gills of the latter had been overlooked
was readily explained by the circumstance that they are retractile, and
not merely contractile. But all the species of the genus Siphonogaster,
or Alma, as it ought really, following the rules of priority, to be called,
have not got gills, as is the case too with the genus Hesperodrilus. The
gills of Alma are branched, and there is therefore no longer any
justification whatever for defining the Oligochaeta as a group of
Annelids without gills. The simple gill-like processes of Chaetobranchus
might have been held to be not accurately comparable to the more
complex structures which we find in the marine worms.
Fig. 189.—Transverse section through Branchiura sowerbyi. × 20. d.br,

Dorsal branchia; i, intestine; n, nerve-cord; v.br, ventral branchia.
Nervous System.—The central nervous system of the Oligochaeta is

very uniform in its structure in the entire group. The only family which is
at all anomalous is that of the Aphaneura. In Aeolosoma there appears
to be only a pair of cerebral ganglia, which retain the primitive position
of these organs in being still in direct connexion with the epidermis. In
all other Oligochaeta there are a pair of cerebral ganglia, connected by
a circumoesophageal commissure with a ventral ganglionated cord.
From the cerebral ganglia arises a system of nerve-fibres and nerve-
cells, which represents the stomatogastric nerves of other
Invertebrates.
Senses and Sense-Organs.—The only organs that can be regarded
with anything like probability as sense-organs are the pigmented eyes
of certain Naids and the tactile cells of many worms. The latter are
usually elongated cells provided at their free extremity with a stiff
process; they occur associated in groups, and often these bundles of
cells have a segmental arrangement. The head end of many of the
lower Oligochaeta, for instance the genus Aeolosoma, has delicate
processes projecting here and there; these appear to be also of a
tactile nature, and are of course connected with cells of the epidermis.
The eyes of certain Naids are little more than lenticular bodies
embedded in a mass of pigment. In the genus Eudrilus and in many
Eudrilidae are peculiar integumental bodies, which were independently
discovered by Dr. Horst[411] and myself, and compared by us to the
Pacinian bodies of Mammals. Whether these structures are connected
with nerves or not is doubtful. In spite of the poor development and the
simplicity of their sense organs, the higher Oligochaeta at any rate can
feel, and can distinguish light from darkness. Darwin[412] came to the
conclusion that "light affects worms by its intensity and its duration."
And furthermore, it is only the anterior end of the body which is thus
affected. Of the sense of hearing these animals appear to be utterly
devoid. Some kept by Darwin "took not the least notice of the shrill
notes from a metal whistle, which was repeatedly sounded near them;
nor did they of the deepest and loudest tones of a bassoon." But it is
always necessary to discriminate between sound and vibrations
passing through any solid body, which would appeal rather to a sense
of touch. Here worms are most sensitive. It is quite easy, by digging
with some vigour, to arouse the worms in the neighbourhood, who will
crawl to the surface and away from the scene of action; a proceeding
on their part which is sometimes put down to a desire to escape from
their enemy the mole.
Smell appears to be another sense which is somewhat deficient. But

worms are epicures, and exhibit a decided taste and preference for
certain articles of diet. Like their fellow tiller of the soil, the agricultural
labourer, worms have a keen relish for onions, which, however, they
must recognise by the smell. They prefer green cabbage to red, celery
to both, and raw meat appears to be the greatest delicacy that can be
offered to them. It is only substances they are not likely to meet with,
such as perfumes, tobacco, and paraffin, that produce no impression
upon the worm's sense of smell.
Coelom and Vascular System.—When an earthworm is dissected the

various organs are seen to lie in a fairly spacious cavity, which is
interrupted and divided into a series of chambers by the mesenteries or
septa which stretch across from wall to wall of the body, and
correspond roughly in their position to the grooves which separate the
body externally. This cavity, common to all the higher animals, is known
as the coelom; it is lined by cells, which cover the intestines as well as
the inside of the body-wall; and upon the intestine assume the form so
characteristic of the group, namely, that of large yellow cells loaded with
secreted matters, and called "chloragogen-cells" by Claparède. The
coelom communicates with the exterior by means of the dorsal pores,
the nephridia, and the ducts of the reproductive organs. As in all
animals which possess a coelom, the reproductive tissues, ova and
sperm, are developed on its walls.
Fig. 190.—Sparganophilus tamesis; general anatomy, × 3. (After

Benham.) I-XVIII, segments. 1, 4, 6, Perivisceral vessels (6 is one of
the hearts); 2, 3, 7, dorsal vessel; 5, spermatheca; 8, sperm sacs; 9,
intestino-tegumentary vessels; 10, ovary; 11, 12, integumentary
vessels.
The vascular system of the Oligochaeta forms a system of perfectly

closed vessels, which ramify into fine capillary networks in the body-
wall, in the coats of the alimentary canal, and upon the other organs of
the body. The main trunks are a dorsal and a ventral longitudinal, which
communicate directly in the anterior end of the body by large transverse
contractile trunks, the so-called hearts (see Fig. 190, 6). The dorsal
vessel is also contractile, but not the ventral, or, when it occurs, the
subnervian. The vascular system has many degrees of complexity in
different families; it is simpler in the smaller aquatic forms. The blood is
usually red, and the pigment which is suspended in the plasma is
haemoglobin. The blood is corpusculated.
Excretory Organs.—There appears to be a great deal more variation

in the structure of the excretory system than there is in many other
groups. For a long time only Lumbricus and a few of the aquatic genera
were known as regards their excretory systems. In these there is a pair
of excretory organs or nephridia in nearly all the segments. These are
much coiled tubes, in which it is always possible to recognise three
divisions. The nephridium commences with an orifice of a funnel-like
character, fringed with long cilia, and opening into the body-cavity; from
this springs a tube, which immediately perforates the septum lying
between the segment which contains the funnel and the following one;
this tube has the peculiarity first pointed out by Claparède of being
excavated in the substance of cells; the glandular part of the
nephridium is a row of cells which are bored through by a continuous
canal, the walls of which are here and there furnished with cilia. It often
happens that the main canal gives off minute lateral ramifications,
which may even form a kind of network round the principal canal. The
terminal section of the nephridium is a muscular sac which opens on to
the exterior by a pore, and from which the products of excretion are
from time to time evacuated by contractions of its walls. This is a brief
statement of the main facts in the structure of those Oligochaeta in
which there is a single pair of nephridia to each segment of the body;
small differences of more or less importance occur. In Chaetogaster, for
example, there is no trace of a funnel; in some genera the terminal sac
is much reduced or unusually extended, being even sometimes
provided with a caecum of moderate dimensions. In Acanthodrilus
novae-zelandiae and a few other species the point of opening of the
nephridia varies from segment to segment, though it always bears
some relation to the chaetae. In these species the nephridia which
open more dorsally are a little different in structure from those which
open more ventrally. One set have a caecum, and the other have not.
The nephridia of the terrestrial forms are enveloped by a richly

developed network of blood capillaries, which is absent in the smaller
aquatic genera.
A very remarkable genus, Brachydrilus, has lately been described by

Dr. Benham,[413] in which each segment has two pairs of nephridia
instead of a single pair. More recently, certain Australian forms, which I
propose to unite on this account into a genus Trinephrus, have been
discovered which have no less than three distinct and separate pairs in
each segment.[414]
Fig. 191.—Section through body-wall of Megascolides australis, highly

magnified. (After Spencer.) 1, 4, 5, 6, Coils of nephridia; 2, funnel; 3,
septum; 7, external apertures.
In many Megascolicidae there is a nephridial system of a different

character. In Perichaeta when dissected the nephridia appear, on
account of their minute size, to be altogether absent. There is, however,
in most Perichaetidae, in many Acanthodrilidae, and in many
Cryptodrilidae a mass of minute tubules which cover the inside of the
body-wall, and open on to the exterior by innumerable openings; there
may be in a single segment one hundred or more of these external
orifices, which are scattered about irregularly. It is at present uncertain
whether these minute tubes are connected among themselves, thus
forming a network passing through the septum and from segment to
segment, or whether each tube is isolated from its fellows, and forms a
distinct nephridium, of which there are many in each segment and
entirely separate. This is, however, certain, that the complex nephridial
systems of at any rate Octochaetus and Megascolides are derived from
the multiplication of a single pair of tubes which are alone present in the
embryo. In Perichaeta the minute nephridia are furnished with coelomic
funnels; in Octochaetus they are not, except in the case of certain
nephridia which open into the terminal section of the intestine.
Both at the anterior and at the posterior end the nephridia occasionally
open into the alimentary canal. In various genera the first pair of
nephridia are larger than the others, and open into the buccal cavity; it
seems likely that they serve as salivary glands. A somewhat similar
condition of things exists in Peripatus (vol. v, p. 17). In Octochaetus
multiporus, for example, there is a large tuft of nephridial tubes in the
anterior region of the body, which opens by a long muscular duct into
the buccal cavity. In the same species a good many of the nephridial
tubes open into the posterior section of the intestine, reminding one of
the anal vesicles of the Gephyrea (p. 436) and of the Malpighian tubes
of the Arthropods.
In many Eudrilidae the ducts of the paired nephridia form a network in

the body-wall, which opens on to the exterior by many pores.
Alimentary Canal.—The digestive tube is perfectly straight in nearly all

Oligochaeta. Only in Plagiochaeta and a species of Digaster is it
twisted in the intestinal region in a corkscrew-like fashion. The mouth is
under the buccal lobe (where, as in the majority of cases, this is
present); the anus is mostly terminal, or rarely, e.g. Criodrilus, a little in
advance of the end of the body on the ventral side. In the simpler forms
three regions can be distinguished, which are themselves simple in
structure. The mouth leads into a buccal cavity, which in its turn opens
into the pharynx; the latter is muscular, with thick walls. The narrower
oesophagus opens into the wider intestine, which opens posteriorly, as
already stated. In the earthworms there is as a rule some complication.
The oesophagus bears certain glandular appendages, the calciferous
glands; and a part of it is modified into a gizzard. The gizzard is merely
a portion of the oesophagus with very much thickened muscular walls
and with a stout lining of chitin. It is not universally present among
earthworms, and when present varies much in position. The rule is that
one gizzard only is present. In Digaster, as is implied by the name, and
in some other forms there are two in successive segments; in Trigaster,
as the name also indicates, there are three gizzards; in Moniligaster
and the Eudrilids Hyperiodrilus and Heliodrilus there are four to six; and
a few other forms also have a considerable number of gizzards. The
calciferous glands are diverticula of the oesophagus with folded and
sometimes ciliated walls; their epithelium secretes calcareous particles,
which are frequently of crystalline form. Darwin supposed that this
secretion was provided in order to negative the humus-acids of the soil
which is the food of earthworms. These organs are usually paired, but
in the Eudrilidae there are unpaired as well as paired glands; the
unpaired calciferous glands lie ventrally. These glands are totally
wanting among the aquatic families, with the sole exception of the
Enchytraeidae. In a few of these there are either paired or single glands
of a very similar nature; Dr. Michaelsen has suggested that the function
of these is rather absorptive than secretory. From the median unpaired
gland of Buchholzia arises the dorsal vessel, which at first forms a
sinus round the glandular epithelium; the epithelium, like that of the
nephridia, is perforated by the ducts. In certain Oligochaeta there are
some curious modifications of the calciferous glands. In Stuhlmannia
and a few other Eudrilidae the oesophagus is beset with a larger
number of paired structures than in any other genera of the family,
where the calciferous glands are more limited in number. These glands
consist of a short tube lined with epithelium opening into the
oesophagus. Round this is a mass of cellular tissue, but the outlines of
the constituent cells are lost; the whole is permeated with abundant
blood-vessels. This layer seems to be peritoneal, and the entire gland
seems to have lost its function as a secretory organ, and to have taken
on some function in connexion with the vascular system. An analogous
modification is to be found among the Enchytraeidae. In certain forms
there is a structure known as the cardiac body; this is a chord of cells
lying in the dorsal blood-vessel at the point where it springs from the
intestine. It is tempting to regard this cellular rod as being the altered
dorsal glandular pouch already spoken of, which is surrounded by a
blood sinus.
Reproductive Organs.—All the Oligochaeta are hermaphrodite

animals. But, as is the case with other hermaphrodites, the male and
female organs are in many cases mature at different times, thus leading
to a practical unisexuality. Many of the aquatic forms appear to have
fixed times for breeding, which may be in the winter or in the summer;
but the earthworms are as a rule sexually mature the whole year round.
Various accessory organs are developed in the majority of cases. In all,
the reproductive glands lie in successive segments and are attached to
the septa, from the peritoneal covering of which they originate. Their
actual position differs greatly in different genera; the position is constant
only in the earthworms, where the testes are in the tenth and eleventh
segments and the ovaries in the thirteenth, in exactly corresponding
situations. A few earthworms have only one pair of testes. The only
exception, among terrestrial forms, to the position of the generative
organs is in the family Moniligastridae, which show so many other
affinities to the lower forms of Oligochaeta. In this family the ovaries
have moved one or two segments forward. Among the fresh-water
families the position of the testes and of the ovaries is not so uniform.
They are generally more anterior than in the terrestrial genera,
particularly the ovaries.
One of the chief differences between the Oligochaeta and the

Polychaeta is that the reproductive organs of the former have special
ducts to convey their products to the exterior. In Aeolosoma, the only
exception to this rule, Dr. Stolc[415] has shown some reasons for
believing that certain nephridia, but slightly altered in form, serve as the
conduits of the spermatozoa, whilst the ova are extruded through a
pore upon the ventral surface of the body. In the Enchytraeidae the
same pore for the extrusion of the ova appears to exist; but a nearer
examination shows that it is really not a mere perforation of the
integument, like the dorsal pores, for example, but that its internal
orifice is fringed with cells which seem to represent a rudimentary
oviduct; perhaps Aeolosoma typifies a last stage in the reduction. Even
so high in the scale as in the genus Nemertodrilus (Eudrilidae), there is
an oviduct which can only be compared with that of the Enchytraeidae.
Elsewhere the oviducts are a pair of tubes with a wide, funnel-shaped,
and ciliated mouth, which leads to the exterior by way of a ciliated tube
of varying length.
The sperm-ducts are of an essentially similar structure; but they are

commonly much longer, passing through a variable number of
segments on their way to the exterior. In most earthworms there are,
moreover, two of them on each side instead of only a single pair, as is
the case with the oviducts. Among the Tubificidae, Naids, and other
aquatic families there are only two sperm-ducts, one on each side of
the body. But this is not a character of the aquatic families, for the
Lumbriculidae have generally two pairs, as in the earthworms. It is,
however, a rule with hardly an exception, that among the aquatic
Oligochaets the sperm-ducts open, as do the oviducts in all
Oligochaets, upon the segments following that which bears internally
the ciliated funnel. It is only in the Moniligastridae among earthworms
that the sperm-duct only traverses two segments in its course. But
where it is short as regards the actual distance traversed between the
two extremities, the tube itself is commonly long and coiled.
Sometimes, as in our common earthworms, the sperm-duct opens

directly on to the exterior of the body, the lips of the external orifice
being swollen by the development of cutaneous gland-cells. In the
majority of cases the sperm-duct or ducts open near or into a glandular
structure which in earthworms has been called "prostate"; in the aquatic
forms, on the other hand, "atrium." As these terms are objectionable
from the different way in which they have been used for structures of
Vertebrates, I have suggested for both the term "spermiducal glands,"
indicating the identity of the structure in all Oligochaeta. The number of
pairs of these glands varies, as does also their shape and size. The
typical form is perhaps illustrated in the lower Oligochaeta, where there
is but a single pair into which the sperm-duct or ducts of the same side
open. The Naids, Tubificidae, Lumbriculidae, and Moniligastridae have
a simple gland of this description on each side of the body. These
glands may consist of a tuft of pear-shaped glandular cells attached to
the organ at one side, as in most Tubificidae, or of a complete
investment of gland-cells, as in Branchiura. Among earthworms it is
only the Moniligastridae and the Eudrilidae in which the sperm-duct
opens directly into the end of the spermiducal gland; in the
Perichaetidae the gland is differentiated into two sections; there is a
muscular duct leading to the exterior, and a lobate glandular part, which
is formed by a complicated branching of a single sac such as exists in
the Tubificidae; in the Acanthodrilidae and in many Cryptodrilidae the
spermiducal glands are of a tubular form and are not branched, though
there is the same differentiation into a duct and a secreting portion.
There are in the Acanthodrilidae two pairs of these, and as many as
three pairs in Dichogaster; in the latter case in three successive
segments. In the Acanthodrilidae the glands are upon the seventeenth
and nineteenth segments. In most Cryptodrilidae the sperm-ducts do
not open into the duct of the spermiducal gland, but on to the body-wall
near to its orifice, the distance varying in different genera. In the
Acanthodrilidae the male pore is on the eighteenth segment, removed
therefore by the distance of a segment from the aperture of either of the
glands. It may be that a large series of structures which exist in
Microchaeta benhami[416] and in other Geoscolecids, and which have
been termed copulatory glands, are the equivalents of the spermiducal
glands.
Fig. 192.—Diagrammatic longitudinal section of Lumbricus, showing the

generative segments. × 3. (After Hesse.) sp, Spermathecal pore; t,
testis; s.s, seminal sac; sp.s, sperm-sac; o, ovary; e.s, egg-sac; ♀ ,
female pore; ♂, male pore.
In many earthworms there are, at the external opening of the male

ducts, bundles of specially modified chaetae, which have been called,
from their supposed function, penial chaetae; they are usually
ornamented at the free end with spinelets or ridges, and frequently offer
valuable specific characters. In the Lumbricidae and the Geoscolicidae
there are modified chaetae upon the clitellum; in a few forms, such as,
for example, Acanthodrilus schmardae, the spermathecae have
bundles of similar chaetae in their neighbourhood, often associated with
glands not unlike the spermiducal glands.
In most, perhaps in all Oligochaeta the sperm is not matured in the

testes, or even in the body-cavity; it is received into special sacs which
are called sperm-sacs, and there ripens. These sacs, the vesiculae
seminales, have been shown to be outgrowths of the septa; their cavity
is thus a portion of the body-cavity shut off more or less completely
from the general body-cavity.
The reproductive organs of the Eudrilidae, and particularly the female

organs, are so divergent in many particulars from those of other
Oligochaeta that it is convenient to treat them separately. The testes
are normal, save that they are often adherent to the posterior wall of
their segment, as, however, is the case with some other earthworms. In
many Eudrilidae, for instance in the genus Hyperiodrilus, the funnels of
the sperm-ducts are dependent from the anterior wall of the segment
which contains them; the narrow tube which follows projects into the
segment in front, and is there immediately dilated into a wide chamber,
which again narrows, and bending round, re-traverses the same
septum; the two ducts of each side (if there are two, which is not
invariably the case) remain separate and open separately into the
glandular part of the spermiducal gland. There is occasionally only a
single median gland; and as a general rule the two glands open by a
median unpaired orifice. Penial chaetae may or may not be present.
Fig. 193.—Female reproductive organs of Hyperiodrilus. XII-XV,

Segments of the body; 1, spermathecal sac; 2, egg-sac; 3,
spermatheca; 4, ovary.
The structure of the female organs differs considerably in detail in the
different genera. But Hyperiodrilus may be taken as an instance of a
genus in which these organs are as complicated as they are anywhere.
The ovaries (Fig. 193, 4) are perfectly normal in structure and in
position. So also are the oviducts; but both are enclosed in sacs which
communicate in rather an elaborate fashion. Each ovisac is somewhat
rounded in form, and the two communicate by a narrow tube; from the
ovisac also arises another narrow tube, which soon dilates into a
chamber lying in the thirteenth segment; this contains the mouth of the
oviduct and is continuous with the egg-sac; the latter is quite normal in
position. Beyond the egg-sacs the two tubes unite round the intestine
and open into a large median sac, which contains sperm and may be
called the spermathecal sac (1). There is, however, a true
spermatheca, single and median. This opens on to the exterior in the
middle of the thirteenth segment, but lies chiefly in the right-hand sac
behind the ovarian portion of the same. I never found this spermatheca
to contain sperm. Dr. Rosa inferred on anatomical grounds, and I have
been able to prove developmentally (in Libyodrilus), that these sacs
which involve the ovaries and oviducts, and which also contain sperm,
are derivatives of the septa; that in fact the spaces which they enclose
are coelomic. In some Eudrilids these sacs are the only
"spermathecae"; in others, as in Hyperiodrilus, there are in addition
blind pouches lying within them which must be regarded as true
spermathecae; these are smaller in some than in others. In fact there
are various transitions in the entire replacement of true spermathecae
apparently homologous with those of other earthworms by pouches
which are derived from the septa, and which are therefore of an entirely
different morphological significance; here is an excellent case of the
substitution of organs, analogous to the replacement of the primitive
notochord of the Vertebrate by the vertebral column.
So far as is known, all the Oligochaeta deposit their eggs in special

chitinous cases, the cocoons. They share this peculiarity with the
Hirudinea. The cocoons have long been known, but were originally
mistaken for the eggs themselves. The cocoons contain several eggs
and a variable quantity of albumen for the nutrition of the growing
embryos. In the majority of earthworms they are more or less oval with
projections at the two ends, and are of a brownish colour. In others the
tint is rather to be described as green. The genera Criodrilus and
Sparganophilus have a cocoon which is greatly elongated. These
structures seem to be undoubtedly formed by the clitellum, the earlier
opinion of D'Udekem being that they were the product of certain glands
developed in Lumbricus at the breeding season, which he thence called
the capsulogenous glands. It is more probable that these glands, which
have been up to the present but little investigated, are the seat of the
formation of the albumen which is found within the cocoons. The
cocoons are deposited at varying depths in the ground, or on the
surface. Among the aquatic genera they are often attached to aquatic
plants. The process of formation has been carefully watched by
Vejdovsky[417] in the genus Rhynchelmis. The worm throws off the
cocoon over its head, crawling backwards to free itself therefrom. The
eggs, spermatozoa and albumen, reach the interior of the cocoon as it
passes over the orifices of the respective ducts. Out of the numerous
eggs which a single cocoon originally contains, only a few, sometimes
only one, reaches to maturity. Among the Enchytraeidae, however,
quite a large number of young emerge from a single cocoon. The
development of all the Oligochaeta is direct, there being no free larval
stage. It seems to be the rule for a process of fission to take place in
the embryos of Allolobophora trapezoides[418] at least, according to the
observations of Vejdovsky, in warm weather. In cold weather he found
in each cocoon as a rule single embryos, and only 10 per cent of
double embryos.
Fig. 194.—Cocoons of Lumbricidae. (After Vejdovsky.) A, Lumbricus

rubellus, nat. size and × 3; B, Allurus, nat. size and × 6; C,
Allolobophora foetida, nat. size and × 3.
Habitat.—Earthworms are found in almost every part of the world

where they have been looked for. They occur far to the north, in Siberia
and Nova Zembla,[419] while South Georgia and Kerguelen mark their

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The Annual Reports in Medicinal Chemistry is dedicated to furthering genuine

A Personal Essay: My Experiences

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disciplines established a powerful approach to drug design that I repeated in

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Soon after the discovery of beta-amyloid-converting enzyme-1

After having succeeded in identifying a potent BACE inhibitor for

Throughout my career, I have been privileged to work with talented and

Natural and Synthetic Neuroactive

Annual Reports in Medicinal Chemistry, Volume 49 # 2014 Elsevier Inc. 27

Due to their potential to modulate the overall excitatory and inhibitory

2. NAS MODULATORS OF THE GABAA RECEPTOR

effects of sulfated NASs on GABA currents.13 On the other hand, benzo-

The affinity of these animals has been much discussed; they

F. E. BEDDARD, M.A. (Oxon.), F.R.S.

OLIGOCHAETA (EARTHWORMS AND THEIR ALLIES)

The Oligochaeta form a well-marked branch of that exceedingly large

Our knowledge of this group, as of so many others, dates from

External Characters.—The most salient external characteristic of this

In some foreign worms the pores of the reproductive ducts are

Structure of the Body-Wall.—The body-wall consists in all

Fig. 187.—Chaetae of Oligochaeta. × 10. (After Michaelsen, Stolc, and

Chaetae.—The passive organs of locomotion in these animals are the

Fig. 188.—Chaetae. × 10. 1, Onychochaeta; 2, Pontoscolex; 3,

In the Tubificid Lophochaeta (Fig. 187, 5) the chaetae are ornamented

Branchiae.—The Oligochaeta were called by Cuvier the "Annélides

Fig. 189.—Transverse section through Branchiura sowerbyi. × 20. d.br,

Nervous System.—The central nervous system of the Oligochaeta is

Smell appears to be another sense which is somewhat deficient. But

Coelom and Vascular System.—When an earthworm is dissected the

Fig. 190.—Sparganophilus tamesis; general anatomy, × 3. (After

The vascular system of the Oligochaeta forms a system of perfectly

Excretory Organs.—There appears to be a great deal more variation

The nephridia of the terrestrial forms are enveloped by a richly

A very remarkable genus, Brachydrilus, has lately been described by

Fig. 191.—Section through body-wall of Megascolides australis, highly

In many Megascolicidae there is a nephridial system of a different

In many Eudrilidae the ducts of the paired nephridia form a network in

Alimentary Canal.—The digestive tube is perfectly straight in nearly all