PMT

Cambridge International Examinations

Cambridge International Advanced Subsidiary and Advanced Level
* 2 6 1 5 4 6 2 0 8 0 *

BIOLOGY 9700/34
Paper 3 Advanced Practical Skills 2 May/June 2016
2 hours
Candidates answer on the Question Paper.
Additional Materials: As listed in the Confidential Instructions.

READ THESE INSTRUCTIONS FIRST

Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use an HB pencil for any diagrams or graphs.
Do not use staples, paperclips, glue or correction fluid.
DO NOT WRITE IN ANY BARCODES.

Answer all questions.

Electronic calculators may be used.

You may lose marks if you do not show your working or if you do not use appropriate units.

At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.

For Examiner’s Use

Total

This document consists of 11 printed pages and 1 blank page.

DC (ST/SW) 105819/3
© UCLES 2016 [Turn over
 PMT

Before you proceed, read carefully through the whole of Question 1 and Question 2.

Plan the use of the two hours to make sure that you finish all the work that you would like to do.

If you have enough time, consider how you can improve the accuracy of your results, for example
by obtaining and recording one or more additional measurements.

You will gain marks for recording your results according to the instructions.

1 The enzyme, E, catalyses the hydrolysis (breakdown) of starch into glucose.

You are required to investigate the progress of this enzyme-catalysed reaction by both

• testing for the disappearance of starch

• testing for the appearance of glucose by finding the time taken for the decolourisation of
potassium manganate(VII) solution.

To follow the progress of this reaction you will need to take samples from the reaction mixture at
different times.

(a) (i) Decide how often you will take these samples including removing the last sample at
20 minutes.

State the times when you will remove the samples.

.......................................................................................................................................[1]

(ii) Decide how you will test a sample to show that the starch has disappeared.

State the reagent you will use .............................................

Describe how you will test each sample to show that the starch has disappeared and
state the colour of any results you might collect.

test ....................................................................................................................................

...........................................................................................................................................

colour ............................................................................................................................[1]

To test for the production of glucose, the change in the colour of potassium manganate(VII)
solution is:

purple colourless

Test-tube Z shows the colourless end-point.

© UCLES 2016 9700/34/M/J/16

 PMT

You are provided with:

labelled contents hazard volume / cm3

E amylase solution harmful 10

irritant

S starch solution none 40

A sulfuric acid irritant 20

P potassium manganate(VII) solution harmful 20

You are advised to wear suitable eye protection, especially when using the amylase solution, E
and the sulfuric acid, A. If either E or A come into contact with your skin, wash off with cold water.
P may stain your skin.

(b) When carrying out a practical procedure the hazards of using the solutions need to be
considered. Then the level of risk needs to be assessed as low or medium or high.

State the hazard with the greatest level of risk when using the solutions then state the level of
risk of the procedure: low or medium or high.

hazard .......................................................................................................................................

level of risk ............................................................................................................................[1]

Proceed as follows:

1. Label the test-tubes with the sampling times that you stated in (a)(i).

2. Put 2.5 cm3 of A into each test-tube.

3. Put 1 cm3 of P into each test-tube and gently shake to mix with A.

(c) Temperature may affect the rate of an enzyme-catalysed reaction.

(i) Use the thermometer to measure the temperature of the room.

State the temperature of the room. ............................ [1]

Read step 4 to step 9 and note that the timer should not be stopped until you have the last end-
point recorded.

The reaction will start as soon as E is added to S.

4. Put 30 cm3 of S into a beaker.

5. Put 4 cm3 of E into the beaker containing S.

6. Immediately stir the mixture in the beaker and start the timer.

© UCLES 2016 9700/34/M/J/16 [Turn over

 PMT

7. At each of your sampling times:

• test for the disappearance of starch as described in (a)(ii)

• test for the appearance of glucose by removing 3 cm3 of the mixture and putting it into
the appropriately labelled test-tube, mixing well.

8. Record, in (c)(ii), the time shown on the timer when the colourless end-point is reached (raw
result). Do not stop the timer.

9. Repeat step 7 and step 8 for each of the times decided in (a)(i) until you have recorded the
end-point for the last sample, removed at 20 minutes.

10. Calculate the time taken to reach each end-point (processed results).

(ii) Prepare the space below to record your results for:

• the test for starch

• the raw results for the appearance of glucose
• your processed results for the appearance of glucose.

[5]

(iii) Now measure the temperature of the room once again.

State the temperature of the room. ............................

State the difference between this temperature and the temperature of the room you
recorded in (c)(i).

...........................................................................................................................................

State whether temperature was a significant error in this investigation.

.......................................................................................................................................[1]
© UCLES 2016 9700/34/M/J/16
 PMT

(iv) Explain how a temperature rise of 30 °C might have affected the mixture of starch and
amylase.

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

.......................................................................................................................................[3]

(v) This procedure investigated the progress of the hydrolysis of starch by amylase.

To modify this procedure for investigating a different variable, the time for the hydrolysis
would be standardised.

Consider how you could modify this procedure to investigate the effect of pH on the
activity of amylase.

Describe how the independent variable, pH, could be investigated.

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

Describe how the dependent variable could be measured.

...........................................................................................................................................

...........................................................................................................................................

.......................................................................................................................................[3]

© UCLES 2016 9700/34/M/J/16 [Turn over

 PMT

(d) A student investigated the effect of starch concentration on the initial rate of reaction of
amylase.

Table 1.1 shows the results for this investigation.

Table 1.1

percentage initial rate of reaction of

concentration of starch amylase
/ arbitrary units
0.00 0
0.50 100
1.25 215
1.75 285
2.50 340
3.25 340

You are required to use a sharp pencil for graphs.

(i) Plot a graph of the data shown in Table 1.1.

[4]

© UCLES 2016 9700/34/M/J/16

 PMT

(ii) Use the graph to estimate the Michaelis-Menten constant (Km).

Show your working on the graph and in the space below.

Km ...........................................................[3]

[Total: 23]

© UCLES 2016 9700/34/M/J/16 [Turn over

 PMT

2 N1 is a slide of a stained transverse section through a plant stem.

You are not expected to be familiar with this specimen.

You are required to use a sharp pencil for drawings.

(a) (i) Select a field of view so that you can observe the epidermis and the vascular bundles.

Draw a large plan diagram of the different tissues in the field of view to show
• two of the large vascular bundles
• the epidermis
• any other observable tissues.

Use one ruled label line and label to identify the xylem.

You are expected to draw the correct shape and proportions of the different tissues.

[5]

© UCLES 2016 9700/34/M/J/16

 PMT

(ii) Observe the cells in the centre of the stem (pith) in N1. These cells are not identical.

Select one group of four adjacent (touching) cells which show some of the differences
between these cells.
Each cell of the group must touch at least two of the other cells.

Make a large drawing of this group of four cells.

Use one ruled label line and label to identify the cell wall of one cell.

[5]

© UCLES 2016 9700/34/M/J/16 [Turn over

 PMT

10

(b) Fig. 2.1 is a photomicrograph of a stained transverse section through a root from a different
type of plant.

You are not expected to be familiar with this specimen.

cell X

610 +m

Fig. 2.1

(i) In Fig. 2.1 a line is drawn across the diameter of a cell, X.

Use this line and the scale bar to find the actual diameter of cell X in μm.

You may lose marks if you do not show your working or if you do not use appropriate
units.

actual diameter .................................................... μm [3]

© UCLES 2016 9700/34/M/J/16

 PMT

11

(ii) Prepare the space below so that it is suitable for you to record the observable differences
between the stem on N1 and the root in Fig. 2.1.

Record your observations in the space you have prepared.

[4]

[Total: 17]

© UCLES 2016 9700/34/M/J/16

 PMT

12

BLANK PAGE

Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every reasonable
effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will
be pleased to make amends at the earliest possible opportunity.

To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge International
Examinations Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download at www.cie.org.uk after
the live examination series.

Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local
Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge.

© UCLES 2016 9700/34/M/J/16