User Manual

RI IMSI™

RESEARCH INSTRUMENTS LTD

Bickland Industrial Park, Falmouth, Cornwall TR11 4TA, UK
t: +44 (0) 1326 372 753 | f: +44 (0) 1326 378 783 |e: [email protected]
www.research-instruments.com

Document 6-50-900UM, Issue 7, 27th April 2015, MRF 2882

RI IMSI™ User Manual
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Introduction
Please read this manual carefully before using the system, and follow the instructions to ensure that
your RI IMSI™ system will work reliably. Obtaining high quality images requires careful set-up and
regular cleaning.
This manual does not contain detailed instructions for using micromanipulators, the microscope or
additional optical components. For detailed information, refer to the individual manuals supplied with
each system component.

Intended Use
To faithfully reproduce the image from a high-resolution microscope on a computer screen, for the
purpose of conducting Intracytoplasmic Morphologically-selected Sperm Injection (IMSI) technique,
during assisted reproduction procedures.

Specifications

DC2 Digital Camera High sensitivity, 1.4 MP, 2/3’’ colour CCD
Monitor 21” 1600 x 1200, DICOM compliant medical imaging monitor
Software RI Viewer ™ software included

Hardware Overview

The quality of the image in an IMSI system is primarily determined by the optical components. IMSI
requires high resolution optics to obtain fine detail, and good contrast enhancement in order for the
detail to be seen easily. Your microscope supplier can provide suitable components.
Resolution does not depend on magnification, but is related to the Numerical Aperture (NA) of the
objective and the condenser. The NAs of both should be as high as possible whilst maintaining practical
working distances.
IMSI works at the limit of what is possible to see with visible light, so, whatever the optics used, they
must be set up exactly according to the instructions supplied. The microscope must also be regularly
serviced and kept clean.

IMSI Monitor

We recommend a monitor designed for medical imaging, and that the PC is fitted with a separate PCI-E
graphics card with DVI (Digital Video Interface) output and at least 256MB memory.
Place the monitor in a convenient position close to the microscope.
Adjust the height and angle of the monitor such that the operator can comfortably look towards the
centre of the screen.

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Monitor Settings (for Medical Monitor Supplied by RI)

The M (Mode) button on the bottom edge of the monitor switches between display modes for different
purposes. Confirm the chosen mode by pressing the l button. We suggest using DICOM mode for IMSI
for the best image contrast and brightness. If the PC is used for other purposes (for example, office
applications), the high brightness and colour temperature of the DICOM mode may be tiring. Change
to Text mode for general computer use.
In Text mode the brightness and colour controls can be adjusted for comfortable viewing. We suggest
setting Text mode to a brightness of 30% with a colour temperature of 6500K.
Brightness and colour cannot be manually adjusted in DICOM mode, as these settings are fixed to
conform to the DICOM standard. See the monitor manual, supplied separately, for detailed instructions.

RI VIEWER™

Introduction

RI Viewer is the software that interacts with the Saturn 5 Laser System for use within an ART laboratory.
Connected to a camera attached to a microscope, it provides on screen images of samples under the
microscope. It can record video and store still images from the microscope in a PC’s file system.
RI Viewer software is provided on a CD and can be downloaded from Software.research-instruments.com.
Once installed the RI Viewer application can be started by double clicking the RI Viewer icon
on the desktop.

User Interface Icons

Icon Meaning Icon Meaning

Take Picture Laser Control

Take Video General Settings

Stop Video Additional Camera

Recording Settings

Open RI Viewer Take Measurements

Gallery

Choose Objective
Display Rulers Tool
Magnification

Increase/Decrease
Instructions For Use
Zoom Level

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How to View Live Images

A camera will need to be connected to the PC running RI Viewer and the drivers installed correctly to
view live images within RI Viewer. The dongle must also be fitted to a USB port.
RI Viewer software will automatically recognise both analogue and compatible digital cameras connected
to the PC. If there is only one camera detected, it will show the live image for that device. If multiple
cameras are connected, it will use the camera that was last selected from the Video Source drop down
box. You may select the device you want the live image to be viewed from. To do this select the camera
from the drop down list on the Settings panel.

How to Zoom & Pan the Image

There are three ways to zoom into an area on the screen.

1. Place the mouse at a point on the screen and click and hold the right mouse button then release.
This will zoom into the spot where the mouse is placed. To zoom out, click and hold the right mouse
button.
2. Place the mouse at a point on the screen and use the scroll wheel on the mouse (if available).
This will give the user control over how much digital zoom is given.
3. Using the tool bar at the bottom of the screen, click the + icon to zoom in incrementally. To zoom
out by the same amount, click on the -- icon on the tool bar. The magnifying glass icon will zoom in
and out by the preset zoom.
Whilst using digital zoom, a thumbnail image of the screen will appear in the top left of the screen. At
the top of this panel the amount of magnification being used is shown, when zoomed out fully, this
panel will not appear on the screen.
Zooming in and zooming out can also be achieved using the foot pedal.

How to Set the Preset Zoom

1. Click the Additional Camera Settings icon on the tool bar.

2. Drag the Preset Zoom slider to the required zoom.

How to Select Cameras

1. Click the General Settings icon on the tool bar. The Video Source drop down box will have a list of
camera names referring to the cameras connected to the PC.
2. Click on the drop down box to allow the selection of cameras. Clicking on the required camera in
the list will change the live image to that of the selected camera.
Note: The list of available cameras is automatically populated to show the attached devices. Camera
names in this list cannot be altered by the user.

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Camera Flipping

1. Click the General Settings icon on the tool bar.

2. Tick Flip Video Horizontal (for cameras that support these options).
3. Tick Flip Video Vertical (for cameras that support these options).

Camera flipping can be used for those microscopes where the camera image is inverted. This often
occurs when the camera is mounted on the eyepiece tube.

Changing Camera Settings

Clicking the Additional Camera Settings icon on the tool bar will show a panel indicating the camera
name and frames per second (fps). Clicking on Additional Settings will allow the user to adjust settings
on the video source. Only settings available to the video source chosen will appear.

Objectives

Objectives can be changed on the microscope to enable the user to get a larger field of view or higher
magnification. If you require accurate measurements of the image, the objective chosen in RI Viewer
must be the same magnification as the objective in use on the microscope.

How to Select Objectives

1. Click the Choose Objective Magnification icon on the tool bar.

2. Click the required objective magnification.

How to Add Objectives

1. Click Display Rulers Tool icon on the tool bar.

2. Click the Objective Calibration button.
3. Click New Objective. The magnification will be the same as the current magnification.
4. Click the required objective to edit Objective Magnification and Fine Adjustment values.

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How to Remove Objectives

1. Click the Display Rulers Tool icon on the tool bar.

2. Click the Objective Calibration button.
3. Click X next to the objective to be removed.

How to Check Objective Calibration

1. Click the Display Rulers Tool icon on the tool bar.

2. Click the Objective Calibration button to open the Objective Calibration panel.

3. Place an object of known dimensions. A stage micrometer

is supplied with each system for this purpose in the field
of view.
Ensure that the objective selected in RI Viewer matches
that being used on the microscope.
4. The stage micrometer supplied measures 100µm between
the longer lines.
5. Drag the rulers to the point from which the stage
micrometer is going to be measured and use the fine
adjustment up and down until the ruler scale matches the
stage micrometer.

How to Configure the Foot Pedal/Keyboard

1. Click the General Settings icon on the tool bar.

2. From the Settings panel click the Keyboard/Foot Pedals Shortcuts.
3. Click the corresponding edit button to assign a shortcut.
4. Hold down the key or press the foot pedal to assign.
5. Click Save and Quit.

Once the shortcuts are set up, press the shortcut keys or the corresponding foot pedal to
activate the command.

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How to Perform Measurements

1. Click the Take Measurements icon on the tool bar.

2. Click on the screen to select a start point for the measurement.
3. Click on the screen a second time to select an end point for the measurement.
The ends of the line can be dragged to change the measurement.
Lines can be removed by clicking the cross in the context window.
Lines are shown during laser operation but cannot be dragged or created and no measurements
will be displayed.
Lines and measurements are shown on an image when images are recorded.

How to Take a Picture

1. Click the Take Picture icon on the tool bar.

2. A camera icon will flash briefly in the centre of the screen to let the user know that an image has
been taken.
Images can be stored in a .bmp file format or .jpeg file format. This is configurable from the Settings
menu by checking compress images.
Images are named by default with the date and time: yyyymmddhhmmss.bmp. For example, a picture
taken on the 3rd of January 2012 at 10:35:02 would be named 20120103103502.bmp.

How to Record Video

1. Click the Take Video icon on the tool bar.

2. Whilst video is being captured, a recording notification will flash in the top left corner
of the screen.
3. Click the Stop Video Recording icon on the tool bar and the recording notification will stop.
Each video recording can last for a maximum of one hour. This eliminates the problem of
accidentally leaving the software recording with the possibility of rendering the computer
unusable.
4. When Freeze Frame is set a keyboard shortcut, the live image can be paused. A second press of
the shortcut key will restart the live image.
Recorded videos are stored in a .wmv file format.
Recorded videos are named by default with the date and time: yyyymmddhhmmss.wmv. For example,
a video taken on the 3rd of January 2012 at 10:35:02 would be named 20120103103502.wmv.

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1. Delete
2. Assign
3. Unselect
4. Select Patient
5. Return to Unassigned Images and Videos or Patient Details
6. Re-Assign
7. Edit Details
8. Copy
9. Full Screen View
10. Print Preview (Images only)

RI Viewer Gallery
The RI Viewer Gallery is used to review, store and add comments to images and videos taken using RI
Viewer. The RI Viewer Gallery icon can be found on the tool bar.

When images and videos are taken with RI Viewer, they are saved to the pre-configured storage folder.
This is configured from the Settings screen of RI Viewer. The RI Viewer Gallery shows all of the images
and videos in this folder. It allows them to be reviewed and then printed, deleted or assigned to a
patient. Once they have been assigned to a patient, they can also have references and comments added.
These can then be printed alongside the images.

The Gallery icon in RI Viewer is used to open RI Viewer Gallery. The first screen displayed is the
Un-Assigned Images screen below.
To return to RI Viewer, close the Gallery using the cross in the top-right corner.

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Un-Assigned Images and Videos

The Un-Assigned Images and Videos screen shows all of the images and videos that are saved in the
default folder as configured in RI Viewer.
• To select an image or video either right-click on it or hold the Ctrl key and left-click. The selected
images are indicated by the coloured border.
• A single left-click will open the image or video in a full-screen view. See “9. Full Screen View”
on page 12.
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Patient Details
The Patient Details screen shows the images that have been assigned to the selected patient along with
the patient name, reference and comments that have been entered for each image.
The Delete, Print, Image Selection and Unselect functions all work in the same way.

RI Viewer Gallery Functions :

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1. Delete
This deletes the selected images and videos. To delete an image or video, first select it, then press
the delete icon. A confirmation screen will appear, allowing the user to confirm whether the images
and videos listed should or should not be deleted.

2. Assign
This is used to assign the selected images and videos to a patient using a unique ID number.
The image or video will be moved to the patient folder.

3. Unselect
This will de-select all of the images and videos that have been selected.

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4. Select Patient
Once images or videos have been assigned to a Patient ID, they are no longer shown on the Un-
Assigned screen. To view the images and videos assigned to a patient click the Select Patient button
to show the Select Patient box. Then enter the patient ID number into the box and click the arrow
to go to the Patient Details screen for that patient. See “Patient Details” on page 9.

5. Return to Unassigned Images and Videos or Patient Details

To return to the Un-Assigned Images and Videos or Patient Details screen click .

6. Re-Assign
If the Patient ID was assigned incorrectly click . A pop-up box will appear in which the
correct patient details relating to the image or video can be input. Click OK to save.

7. Edit Details
This is used to enter a patient name, reference and a comment. These are saved with each image
and are displayed on the Patient Details screen.
If a different patient name, reference or comment has been entered across a selection of images,
the Edit Details screen will display multiple values for these fields. Changing this will alter the value
for all of the selected images.

8. Copy
This copies the selected images and videos to the clipboard. These images can then be pasted to a
new location such as a USB drive or into another application such as a word processor.

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9. Full Screen View

A single left-click on an image or video will open it in a full-screen view. Delete, Assign, Re-Assign,
Copy and Print can all be actioned from this view. To return to the Un-Assigned Images and Videos
or Patient Details screen click .
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10. Print Preview

To preview the image before printing, first select the image. Then press Print.
This will open the Print Preview Functions.

A. Print
B. Print Setting

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A. Print
The Print button will print the selected images with the current settings. See “B. Print Settings” below.

B. Print Settings
Select the printer, paper size, page range, etc required for printing the selected images.
Press OK to save the changes and return to Print Preview or Cancel to discard your changes.

Advice for Use

All images and videos should eventually be moved out of the Un-Assigned list so that it only contains
newly captured images and videos. If there are any that you do not wish to delete permanently or assign
to an existing patient, then a patient ID of ‘miscellaneous’ or similar can be created to assign them to.
Whenever one or more videos are selected, either in addition to a number of images or by themselves,
the print command is unavailable.

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Maintenance

Monitor Calibration
The monitors we supply will provide a high quality image with minimal set up. However, performance
of any monitor will change slowly over time, and periodic calibration will ensure a high quality image
for as long as possible.
Larger hospitals may have calibration facilities already in place to cover monitors used for X-ray imaging.
Contact your IT/maintenance department for information.
Calibration involves measuring the screen output using a colorimeter. The measurements are stored
by the graphics card and used to compensate for any slight errors. Note: The colorimeter is specific
for DICOM calibration on these monitors. General purpose colorimeters (eg Datacolor Spyder3)
are not compatible.
Colorimeters can be supplied by RI, or a calibration service may be offered by your RI distributor or local
representative of the monitor manufacturer. We suggest calibrating your monitor every twelve months.

Troubleshooting

Problem Possible Cause Solution

Ensure USB cable is properly connected
Camera not connected
and undamaged
No video image
Ensure the microscope‘s port selector is
Port selector closed
set to the appropriate position
Correction collar out of Turn the correction collar on the
adjustment objective to the correct position
Make sure all accessible optical surfaces
Poor quality image Dirt on optics
are perfectly clean
Optics system out of alignment Refer to microscope provider
Monitor in Text mode Set monitor to DICOM mode
Excessive zoom cannot resolve more
Blurred image Too much digital zoom
detail. Reduce zoom level

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Software Updates
Updates to RI Viewer software can be obtained by visiting http://software.research-instruments.com/
and clicking on the link for RI Viewer.

RI Repairs System
In the event that you have a problem with an RI instrument, please follow the procedure below to
ensure prompt attention.
1. Read the ‘Troubleshooting’ section.
2. If you require any further help contact your distributor or RI directly.
RI will try to resolve the problem as quickly as possible.

RI Returns System
1. Contact RI to obtain a Returned Materials Authorisation (RMA) number. Note: Goods will not be
replaced or refunded without prior agreement and clearly stating the RMA number.
2. Pack the item carefully in its original packaging. RI will not accept responsibility for damage due to
incorrect packaging. Replacement items or additional repairs will be invoiced.
3. Clearly label the package with the RMA number, mark the package “Urgent - Returned Items For
Repair”, and ship to the address on the next page. Goods should be insured for their full value
during shipping.

Contact Details
Research Instruments Ltd, Bickland Industrial Park,
Falmouth, Cornwall, TR11 4TA, UK
Tel: +44 (0) 1326 372 753 Fax: +44 (0) 1326 378 783
E-mail: [email protected]
Website: www.research-instruments.com

Feedback
Thank you for purchasing a RI product. To help RI develop the best tools for ART, we rely on customer
feedback. If you have any suggestions for how we can improve our products or the information we
provide with them, please send them to [email protected]. Your feedback will help
us develop the product and supporting materials to meet your future needs.
Thank you.

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