WHO SPECIFICATIONS AND EVALUATIONS FOR PUBLIC HEALTH PESTICIDES

d-ALLETHRIN1
(RS)-3-allyl-2-methyl-4-oxocyclopent-2-enyl (1R)-cis, transchrysanthemate

2002

WORLD HEALTH ORGANIZATION Geneva

d-Allethrin is the name given by the manufacturer, in the absence of an ISO common name

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TABLE OF CONTENTS d-ALLETHRIN

DISCLAIMER INTRODUCTION PART ONE SPECIFICATIONS FOR d-ALLETHRIN d-ALLETHRIN INFORMATION d-ALLETHRIN TECHNICAL MATERIAL PART TWO 2002 EVALUATION REPORT FOR d-ALLETHRIN, INCORPORATING FOOTNOTES ADDED IN 2003 and 2004

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Disclaimer1

WHO specifications are developed with the basic objective of promoting, as far as practicable, the manufacture, distribution and use of pesticides that meet basic quality requirements. Compliance with the specifications does not constitute an endorsement or warranty of the fitness of a particular pesticide for a particular purpose, including its suitability for the control of any given pest, or its suitability for use in a particular area. Owing to the complexity of the problems involved, the suitability of pesticides for a particular purpose and the content of the labelling instructions must be decided at the national or provincial level. Furthermore, pesticides which are manufactured to comply with these specifications are not exempted from any safety regulation or other legal or administrative provision applicable to their manufacture, sale, transportation, storage, handling, preparation and/or use. WHO disclaims any and all liability for any injury, death, loss, damage or other prejudice of any kind that may be arise as a result of, or in connection with, the manufacture, sale, transportation, storage, handling, preparation and/or use of pesticides which are found, or are claimed, to have been manufactured to comply with these specifications. Additionally, WHO wishes to alert users to the fact that improper storage, handling, preparation and/or use of pesticides can result in either a lowering or complete loss of safety and/or efficacy. WHO is not responsible, and does not accept any liability, for the testing of pesticides for compliance with the specifications, nor for any methods recommended and/or used for testing compliance. As a result, WHO does not in any way warrant or represent that any pesticide claimed to comply with a WHO specification actually does so. ____________________________________

1 This disclaimer applies to all specifications published by WHO.

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INTRODUCTION WHO establishes and publishes specifications* for technical material and related formulations of public health pesticides with the objective that these specifications may be used to provide an international point of reference against which products can be judged either for regulatory purposes or in commercial dealings. From 2002, the development of WHO specifications has followed the New Procedure, described in the 1st edition of Manual for Development and Use of FAO and WHO Specifications for Pesticides (2002). This New Procedure follows a formal and transparent evaluation process. It describes the minimum data package, the procedure and evaluation applied by WHO and the experts of the FAO/WHO Joint Meeting on Pesticide Specifications (JMPS). WHO Specifications now only apply to products for which the technical materials have been evaluated. Consequently, from the year 2002 onwards the publication of WHO specifications under the New Procedure has changed. Every specification consists now of two parts, namely the specifications and the evaluation report(s): Part One: The Specification of the technical material and the related formulations of the pesticide in accordance with chapters 4 to 9 of the 1st edition of the FAO/WHO Manual on Pesticide Specifications. Part Two: The Evaluation Report(s) of the pesticide, reflecting the evaluation of the data package carried out by WHO and the JMPS. The data are provided by the manufacturer(s) according to the requirements of chapter 3 of the FAO/WHO Manual on Pesticide Specifications and supported by other information sources. The Evaluation Report includes the name(s) of the manufacturer(s) whose technical material has been evaluated. Evaluation reports on specifications developed subsequently to the original set of specifications are added in a chronological order to this report. WHO specifications developed under the New Procedure do not necessarily apply to nominally similar products of other manufacturer(s), nor to those where the active ingredient is produced by other routes of manufacture. WHO has the possibility to extend the scope of the specifications to similar products but only when the JMPS has been satisfied that the additional products are equivalent to that which formed the basis of the reference specification.

* Footnote: The publications (http://www.who.int/ctd/whopes).

are

available

on

the

Internet

under

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PART ONE SPECIFICATIONS

d-ALLETHRIN d-ALLETHRIN INFORMATION d-ALLETHRIN TECHNICAL MATERIAL

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WHO SPECIFICATIONS AND EVALUATIONS FOR PUBLIC HEALTH PESTICIDES

d-ALLETHRIN

INFORMATION
Common name Chemical name No ISO common name for d-allethrin. Footnote 1 CAS: none IUPAC: (RS)-3-allyl-2-methyl-4-oxocyclopent-2-enyl (1R)-cis, transchrysanthemate CAS Registry number CIPAC number Structural formula
H COO 1R,trans;1R O H COO 1R,trans;1S O

No CAS number available for d-allethrin. Footnote 2 742

H COO 1R,cis;1R O

H COO 1R,cis;1S O

d-allethrin consists of [1R,trans;1R] + [1R,trans;1S] +[1R,cis;1R] + [1R,cis;1S] in an approximate ratio of 4:4:1:1

Molecular formula Relative molecular mass

C19H26O3 302.41

1 2

Allethrin is the ISO common name for the racemic mixture of 8 stereoisomers.

CAS number for racemic allethrin: 584-79-2 (unstated stereochemistry and also used for bioallethrin).

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d-ALLETHRIN TECHNICAL MATERIAL WHO Specification WHO/742/TC (2002)

This specification, which is PART ONE of this publication, is based on an evaluation of data submitted by the manufacturer whose name is listed in the evaluation report (742/2002). It should be applicable to relevant products of this manufacturer but it is not an endorsement of those products, nor a guarantee that they comply with the specifications. The specification may not be appropriate for the products of other manufacturers. The evaluation report (742/2002) as PART TWO forms an integral part of this publication.

1 Description The material shall consist essentially of d-allethrin with related manufacturing impurities. It shall be a yellowish-brown oil, substantially odourless and free from extraneous materials or added modifying agents. 2 Active ingredient 2.1 Identity tests (Note 1) The active ingredient shall comply with an identity test and, where the identity remains in doubt, shall comply with at least one additional test. 2.2 d-Allethrin content (CIPAC 741/TC/M/-) (Note 2) The d-allethrin content shall be declared (not less than 900 g/kg) and, when determined, the average measured content shall not be lower than the declared minimum content. 2.3 Isomer composition (Note 2) The trans-isomer content of the d-allethrin shall be declared (not less than 75% and not more than 85%) and, when determined, the average measured trans-isomer content shall not be lower than the declared minimum value nor higher than the declared maximum value. The 1R-isomer content of the d-allethrin shall be declared (not less than 95%) and, when determined, the average measured 1R-isomer content shall not be lower than the declared minimum content. 3 Relevant impurities 3.1 Chrysanthemic anhydride (Note 3) CAS: (2,2-dimethyl-3-(2-methyl-1propenyl) cyclopropanecarboxylic anhydride. CAS No. 14297-82-6) Maximum: 10 g/kg. _______________________________________
Note 1 Identity tests based on GC retention time (see Note 2) or IR spectrum provide evidence of identity as allethrin enantiomers but determination of the isomer composition (see Note 2) provides the definitive identity test for d-allethrin.

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IR spectrum of d-allethrin (identifies the presence of allethrin enantiomers)

Note 2

Methods for the determination of d-allethrin content and isomer composition were adopted by CIPAC in 2003 but are not yet published. Prior to publication in CIPAC Handbook L, copies of the methods may be obtained through the CIPAC website, http://www.cipac.org or from the Secretary, Dr Lszl Bura, Central Service for Plant Protection and Soil Conservation, Budarsi t 141-145, 1118 Budapest, Hungary. Determination of chrysanthemic anhydride content. Scope of method The method is intended for the determination of chrysanthemic anhydride in d-allethrin TC. It is a relatively non-specific technique but has been shown to be satisfactory for the intended purpose. It is not intended for the determination of chrysanthemic anhydride in d-allethrin formulations. Outline of method Chrysanthemic anhydride in d-allethrin TC is reacted with excess morpholine. The amounts of morpholine added to, and remaining after, the reaction are determined with 0.1 mol/l HCl-methanol solution. The amount of morpholine consumed in the reaction, determined as hydrochloric acid equivalent, is used to calculate the content of chrysanthemic anhydride. Reagents Hydrochloric acid, concentrated Methanol Ethanol Morpholine Methyl red Methyl yellow Methylene blue Sodium carbonate, volumetric analysis grade Hydrochloric acid-methanol solution (approximately 0.1 mol/l). hydrochloric acid (9.5 ml) to 1000 ml with methanol. Dilute concentrated

Note 3

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Morpholine-methanol solution (approximately 0.1 mol/l). Weigh or dispense morpholine (about 8.7 g or 8.7 ml) and dilute to 1000 ml with methanol. Methyl red indicator solution. Dissolve methyl red (0.1 g) in ethanol (100 ml) and filter if necessary. Methyl yellow-methylene blue indicator solution. methylene blue (0.1 g) in methanol (125 ml). Procedure Standardization of 0.1 mol/l hydrochloric acid-methanol solution. Weigh accurately sodium carbonate (0.15 g), previously heated to between 500C and 650C for 40 to 50 min and allowed to cool in a desiccator. Dissolve in water (30 ml), add methyl red indicator solution (3 drops) and titrate the solution with 0.1 mol/l hydrochloric acidmethanol until the colour of the solution changes to persistent orange to orange-red. Calculate the normality factor (f) using the following equation. f = where a V a V x 5.299 = amount (mg) of sodium carbonate, corrected for purity; = volume (ml) of 0.1 mol/l hydrochloric acid-methanol solution; Dissolve methyl yellow (1 g) and

5.299 = mass of sodium carbonate equivalent to 0.1 mole of hydrochloric acid (105.989 2 x 0.1). Determination. Weigh accurately 2.0 g of sample into a conical flask (100 ml). Add 25 ml morpholine-methanol solution and dissolve the sample completely. Add methyl yellow-methylene blue indicator solution (3 to 4 drops) and titrate with 0.1 mol/l hydrochloric acid-methanol until the colour of the solution changes from green to red. Perform a blank determination in the same manner, adding exactly the same volume of morpholine solution. Calculation Chrysanthemic anhydride (g/kg) = 31.846 x (B A) x f x 1000 W where A B f W = the volume of 0.1 mol/l hydrochloric acid-methanol solution used to titrate the sample solution; = the volume of 0.1 mol/l hydrochloric acid-methanol solution used to titrate the blank solution; = the normality factor for the 0.1 mol/l hydrochloric acid-methanol solution; = the amount (mg) of the test sample;

31.846 = mass of chrysanthemic anhydride equivalent to 0.1 mole of hydrochloric acid (318.46 x 0.1).

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PART TWO EVALUATION REPORT(S)

d-ALLETHRIN

2002

Evaluation report based on submission of data from Sumitomo Chemical Company Ltd, incorporating footnotes added in 2003 and 2004. (TC)

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WHO SPECIFICATIONS AND EVALUATIONS FOR PUBLIC HEALTH PESTICIDES d-ALLETHRIN EVALUATION REPORT WHO/742/2002 Explanation d-Allethrin was evaluated by the WHO/IPCS in 1989 (IPCS, 1989a). It was reviewed by US EPA in 1975 and by the Health and Safety Executive in UK prior to 1985. The draft specification and the supporting data were provided by Sumitomo Chemical Company Ltd., Japan, in 2001. The patent for d-allethrin has expired. Uses d-Allethrin is a synthetic pyrethroid with fast knock down activity against household pest insects. It is used in public health against mosquitoes, houseflies and cockroaches1. Identity ISO common name None (footnote 2) Chemical name IUPAC: (RS)-3-allyl-2-methyl-4-oxocyclopent-2-enyl (1R)-cis, transchrysanthemate CA: None (footnote 3) CAS Registry number None (footnote 4) CIPAC number 742 Synonyms
1 2

2003 footnote. The proposer stated that d-allethrin is also registered for use on garden plants. Allethrin is the ISO common name for the racemic mixture of 8 stereoisomers; d-allethrin is the name given by the Sumitomo Chemical Company to the specific ratio of isomers defined by the WHO specification. Proposed CAS name: 2-methyl-4-oxo-3-(2-propenyl)-2-cyclopent-1-yl methyl-1-propenyl) cyclopropanecarboxylate. The CAS number for allethrin is 584-79-2. 2,2-dimethyl-3-(2-

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Pynamin Forte Structural formula

H COO 1R,trans;1R O

H COO 1R,trans;1S O

H COO 1R,cis;1R O

H COO 1R,cis;1S O

d-allethrin consists of [1R,trans;1R] + [1R,trans;1S] +[1R,cis;1R] + [1R,cis;1S] in an approximate ratio of 4:4:1:1

Notes on the isomer composition of some related active ingredients: allethrin is a racemic mixture of the 8 stereoisomers; bioallethrin consists of [1R,trans:1R] and [1R,trans;1S] isomers, approx ratio 1:1; esbiothrin consists of [1R,trans:1R] and [1R,trans;1S] isomers, approx ratio 1:3; S-bioallethrin (esbiol) is the [1R,trans;1S] isomer.

Molecular formula C19H26O3 Relative molecular mass 302.41 Identity tests (1) isomer composition (enantio-specific)1; (2) GC retention time in the analytical method, capillary GC with FID (not enantio-specific); (3) IR spectrum (not enantio-specific).

2003 footnote. The isomer composition is determined by enantio-selective HPLC and not, strictly, enantio-specific HPLC.

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Physico-chemical properties of pure d-allethrin Table 1. Physico-chemical properties of pure d-allethrin

Parameter Vapour pressure: Melting point and temperature of decomposition: Solubility in water: Value(s) and conditions see vapour pressure for technical material Melting point: not applicable Decomposition temperature: not available 5.00 0.11 mg/l at 25 C and pH 5.9 to 6.0 Note1 Purity % Method reference Not applicable

99.5

EPA Guideline CG1500 (Saito et al., 1989a) OECD 107 (Saito et al., 1989b)

Octanol / water log POW = 4.95 (4.89 to 4.97) at partition coefficient: 25 C and pH 5.8 to 6.0. Initial concentration in octanol = 738 to 3000 mg/l. Hydrolysis characteristics: d-trans-allethrin2

99.5

No measurable hydrolysis after 31 Radiochemical days at 25 C and pH 5. purity: 99.3 Estimated half-life approx 500 days at 25 C and pH 7. Half life: 4.3 days at 25 C and pH 9. Radiochemical Photodegradation in water under purity: 99.3 natural sunlight: Half life: 49 experiment hours or 19 sunlight hours at 25.5C and pH 5 Not applicable

EPA Guideline 1611 (Estigoy et al., 1990)

Photolysis3 characteristics: d-trans-allethrin Dissociation characteristics:

EPA Guideline 1612 (Chari et al., 1990)

Not applicable

1 2

Water solubility: observed values, 4.81-5.24 mg/l (n=18). Hydrolysis rates were measured at 0.5 mg/l in 1% aqueous acetonitrile in sterile dark conditions for 1 month at pH 5 and pH 7 and for 16 days at pH 9. Hydrolysis products were identified after pH 9 hydrolyis, allethrolone and two isomeric bicyclic ketones. Photolysis rates were measured at 0.5 mg/l in 1% aqueous acetonitrile in sterile buffer at pH 5 and 25.5C. Quartz tubes of solution were exposed to sunlight at 37.45N (Richmond, California) for 5 days in January. Photolysis products were identified as allethrolone, dihydroxyallethrolone and carbon dioxide. Cis-trans isomerization was not observed under the conditions.

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Chemical composition and properties of d-allethrin technical material (TC) Table 2. Chemical composition and properties of d-allethrin TC
Manufacturing process, maximum limits for impurities 1 g/kg, 5 batch analysis data. Declared minimum d-allethrin content: Relevant impurities 1 g/kg and maximum limits for them: Relevant impurities < 1 g/kg and maximum limits for them: Stabilizers or other additives and maximum limits for them: Boiling temperature range1 test guideline: 40 CFR 158 Confidential information supplied and held on file by WHO. Mass balances were 98.7 to 99.5% and percentages of unknowns were 0.5 to 1.3%. 900 g/kg. Chrysanthemic anhydride = 10g/kg. None. None. Boiling point (Pynamin forte): 281.5C at 760 mm Hg. Observed values 282.2, 281.1, 282.2C at 765.58 mm Hg. At approximately 199C, the test material changed from a pale yellow to a dark yellow colour and, at 232C, it turned brown and began to smoke. (Hoffman, 1989). Pynamin forte: 1.65 10-4 Pa at 21.6 C. Observed values: 1.45 10-4, 1.91 10-4, 1.60 10-4 Pa at 3 gas flow rates. (Semann et al., 1989).

Vapour pressure2 test guideline: 40 CFR 158

Boiling point measurements were made in a Siwoloboff boiling point apparatus. Purity of the material was not stated. The bath liquid was heated at about 3C/min until 10C below the expected boiling point, when the rate was reduced to 1C/min. Vapour pressure was measured by a gas-saturation method. Dry nitrogen gas streams were passed through gas-saturation tubes at 21.6C and then through traps in dry ice/ethanol for approx 5 days at flow rates of 4.4, 5.2 and 7.6 ml/min. The contents of the traps were measured by HPLC.

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Hazard summary
Notes. (i) The proposer provided written confirmation that the toxicological and ecotoxicological data included in the summary below were derived from d-allethrin having impurity profiles similar to those referred to in the table above. (ii) The conclusions expressed in the summary below are those of the proposer, unless otherwise specified.

Table 3. Toxicology profile of d-allethrin technical material, based on acute toxicity, irritation and sensitization.
Species Rats, male and female Rabbits, male and female Rats, male and female Rabbits, male and female Rabbits, male and female Guinea pigs Test oral dermal Duration and conditions EPA Guideline 81-1 EPA Guideline 81-2 EPA Guideline 81-3 EPA Guideline 81-5 EPA Guideline 81-4 Buehler method, EPA Guideline 81-6 Result LD50 = (male) 2150, (female) 900 mg/kg bw Purity Ref.

92.4% Hiromori et al., 1989a

LD50 = (male) 2660, 92.4% Hiromori et al., (female) 4390 mg/kg bw 1989b LC50 = (male) >3875, (female) >3875 mg/m3 Not irritant 92.4% Kawaguchi et al., 1989 92.4% Nakanishi et al., 1988 92.4% Nakanishi et al., 1988 92.4% Nakanishi et al., 1989

inhalation skin irritation

eye irritation

Minimal irritant

skin sensitization

Not sensitizing1

2003 footnote. The concentration of chrysanthemic anhydride in the test material was 6 g/kg.

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Table 4. Toxicology profile of d-allethrin technical material based on repeated administration (subacute to chronic)
Species Test Duration and conditions 3 months EPA Guideline 82-4 3 months Result Purity Reference

Rats, male and female Rats, male and female Mice, male and female Dogs, male and female Dogs, male and female Rats, male and female

inhalation

NOEL = both sexes: 50.0 mg/m

92.3%

Kawaguchi et al., 1993

feeding, toxicity

NOEL = 750 ppm diet, equivalent to: male: 49.6, female: 59.2 mg/kg/day NOEL = both sexes: 100 ppm , equivalent to: male: 14, female: 16 mg/kg/day NOEL = both sexes: 10 mg/kg/day NOEL = both sexes: 6 mg/kg/day

94.5%

Kadota, 1977 IPCS, 1989, p40 Edwards et al., 1986

feeding, toxicity

5 weeks

93.4%

feeding, toxicity

4 weeks

93.1%

Dalgard et al., 1987 Dalgard et al., 1989

feeding, toxicity

1 year EPA Guideline 83-1 123 weeks

Not reported

feeding, carcinogenicity

NOEL = 125 ppm in diet, equivalent to: male: 5.9 mg/kg/day, female: 6.6 mg/kg/day. Carcinogenicity: negative NOEL = male: 600 ppm, female: 600 ppm. Carcinogenicity: negative Reproduction NOEL = 6000 ppm. Adult and fetus NOEL = 200 ppm Maternal NOEL = 30 mg/kg. Developmental NOEL = 100 mg/kg Maternal NOAEL = 100 mg/kg Developmental NOAEL = >100 mg/kg

91.4%, 92.2%

Arai et al., 1985 IPCS, 1989, p45

Mice, male and female

feeding, carcinogenicity

81 weeks EPA Guideline 83-2 EPA Guideline 83-4 EPA Guideline 83-3 EPA Guideline 83-3

93.1%

Mayfield et al., 1989e

Rats, male and female Rats, male and female Rabbits, male and female

feeding, 2 generation reproduction feeding, teratogenicity and embryotoxicity feeding, teratogenicity and embryotoxicity

93.4%

Hoberman, 1989a

93.4%

Hoberman, 1989b

93.4%

Hoberman, 1989c

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Table 5. Mutagenicity profile of d-allethrin technical material based on in vitro and in vivo tests
Species Salmonella typhimurium, Escherichia coli Rat hepatocytes Chinese hamster ovary cells Test Gene mutation Conditions Result Purity 93.4% Reference Kogiso et al., 1989a Kogiso et al., 1989b Kogiso et al., 1989c Ames test/In Negativ vitro e In vitro In vitro Negativ e Negativ e

DNA damage and repair Mammalian chromosomal aberration test

93.4%, 91.9% 93.4%, 91.9%

Table 6. Ecotoxicology profile of d-allethrin and related technical materials.

Species Test Duration and conditions 8 days Result Purity Reference

Bobwhite

Acute dietary toxicity Acute dietary toxicity Acute flowthrough toxicity Acute flowthrough toxicity Acute flowthrough toxicity Acute flowthrough toxicity Acute flowthrough toxicity Acute flowthrough toxicity Static Contact toxicity

LC50 : >5620 ppm LC50 : >5620 ppm LC50 (96 hr): 9.40 g/L LC50 (96 hr): 9.70 g/L LC50 (96 hr): 27.0 g/L LC50 (96 hr): 9.90 g/L LC50 (96 hr): 53 g/L LC50 (96 hr): 14.6 g/L LC50 : 25 mg/L

d-allethrin 93.4%

Mallard

8 days

d-allethrin 93.4%

Coho salmon Steelhead trout Channel catfish Yellow perch Fathead minnow Channel catfish Daphnia pulex Honey-bee

96 hr at 12C 96 hr at 12C 96 hr at 12C 96 hr at 12C 96 hr at 12C 96 hr at 12C 3 hours

d-trans-allethrin 90% d-trans-allethrin 90% d-trans-allethrin 90% d-trans-allethrin 90% S-bioallethrin 98% S-bioallethrin 98%

Honey-bee Oral toxicity

bioallethrin, purity not stated LD50 3.4 g/bee allethrin, purity not stated LD50 4.6allethrin, purity not stated 9.1 g/bee

Fink and Beavers, 1978a Fink and Beavers, 1978b Mauck et al., 1976. Mauck et al., 1976. Mauck et al., 1976. Mauck et al., 1976. Mauck et al., 1976. Mauck et al., 1976. IPCS, 1989, p36 IPCS, 1989, p37 IPCS, 1989, p37

d-Allethrin was evaluated by the WHO/IPCS in 1989, with the following conclusions(IPCS, 1989, p54). General population: Under recommended conditions of use, the exposure of the general population to allethrins is negligible and is unlikely to present a hazard. Occupational exposure: With reasonable work practices, hygiene measures, and safety precautions, the use of allethrins is unlikely to present a hazard to those occupationally exposed to them.

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Environment: Under recommended conditions of use and application rates, it is unlikely that allethrins or their degradation products will attain significant levels in the environment. In spite of the high toxicity of these compounds for fish and honey-bees, they are only likely to cause a problem in the case of spillage or misuse. The WHO hazard classification of d-allethrin is: slightly hazardous. Formulations The main formulation types available are MC (mosquito coils) and MV (vaporizing mats). These formulations are registered and sold in many countries throughout the world. Methods of analysis and testing The validation of the analytical method for the active ingredient (including identity tests) is in progress under the auspices of CIPAC. The d-allethrin is determined by capillary GC with FID. The method was submitted to JMPS in 2002 (Furuta, 2002)1. Analytical methods for isomer composition are available (Fujita, 2002a). The optical isomer ratios are determined by HPLC using a chiral stationary phase, while the geometric isomer ratio is determined by GLC on a non-chiral stationary phase. The methods have been validated by Sumitomo Chemical Co Ltd. The isomer composition determined by these methods provides the critical identity test for d-allethrin2. The analytical method for the determination of the relevant impurity, chrysanthemic anhydride, in d-allethrin TC is available (Fujita, 2002b) but it has not yet been peer-validated. The method relies on the back-titration of residual morpholine after standard addition to the sample.3 An additional (non-stereospecific) identity test relies on matching the IR spectrum of the test sample with the IR spectrum for d-allethrin. Test methods for determination of physico-chemical properties of technical active ingredient were OECD and EPA, while those for the formulations were in accordance with the WHO guideline specifications for household insecticide products.

2003 footnote. The analytical method for determination of d-allethrin content was successfully validated and adopted by CIPAC in June, 2003. 2003 footnote. The identity test for determination of trans- and 1R isomer ratios was successfully peer validated and adopted by CIPAC in June, 2003. 2003 footnote. The analytical method for determination of chrysanthemic anhydride in d-allethrin TC was successfully peer validated and is appended to the specification.

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Containers and packaging No special requirements for containers and packaging have been identified. Expression of the active ingredient The active ingredient is expressed as d-allethrin. Appraisal The data submitted were in accordance with the requirements of the FAO Manual (5th edition) and supported the draft specifications. No common name is available for d-allethrin; d-allethrin is the name given by the manufacturer. The technical material, d-allethrin is a mixture of [1R,trans;1R] + [1R,trans;1S] + [1R,cis;1R] [1R,cis:1S] allethrin in an approximate ratio of 4:4:1:1. The CIPAC number for d-allethrin is 742. Allethrin and bioallethrin have CIPAC numbers 267 and 203, respectively. A CAS name and a number are available for allethrin, but not for d-allethrin. d-Allethrin is out of patent. d-Allethrin is slightly volatile at room temperature (vapour pressure 0.165 mPa at 25C) with a boiling point of 281.5C. It has low water solubility (5 mg/l at 25C) and the octanol-water partition coefficient (log POW = 4.95 at 25C) makes d-allethrin a fat-soluble compound. It is stable to hydrolysis under neutral or slightly acidic conditions, but hydrolyses readily under basic conditions. Photolysis in water degrades d-trans-allethrin quickly; cis-allethrin is not detected as a significant product. The hydrolysis study used d-trans-allethrin, which comprises 80% of d-allethrin active ingredient, so the results should be generally transferable to d-allethrin. The photolysis study also used d-trans-allethrin. The Meeting was provided with commercially confidential information on the manufacturing process and batch analysis data on all impurities present at or above 1 g/kg. Analyses of 5 batches of d-allethrin produced in 2000 and 2001 accounted for 98.7 to 99.5% of the material, including 2.0 to 2.2% of higher molecular weight unknowns. Chrysanthemic anhydride was identified by the proposer as a relevant impurity because it has a potential for skin sensitization. The opinion of WHO/PCS was that chrysanthemic anhydride should be considered a relevant impurity, on a precautionary basis, but that further information is required to define the risks involved and to support the limit proposed. Following a post-meeting consideration of all data available on the hazards presented by chrysanthemic anhydride and the associated risks, agreement was reached between WHO/PCS and the proposer that the maximum level of chrysanthemic anhydride in

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d-allethrin TC should be 10 g/kg1,2. The highest level of chrysanthemic anhydride in the 5-batch analyses was 7 g/kg. The concentration of chrysanthemic anhydride in d-allethrin TC is measured by a back-titration method. This method may be unsuitable for the determination of chrysanthemic anhydride in d-allethrin formulations but specifications for such formulations were not proposed to the meeting. Given the low level of active ingredient in the MV (vaporizing mats) and MC (mosquito coil) formulations and very low probability of substantial dermal contamination of users, WHO/PCS concluded that chrysanthemic anhydride should not be considered a relevant impurity in these formulations. Therefore, if specifications for these formulations are proposed in future, it would not be necessary to develop a more sensitive and specific method for the determination of chrysanthemic anhydride in such products. The impurity profile data submitted to WHO were declared by the Australian National Registration Authority for Agricultural and Veterinary Chemicals (NRA) to be identical to those submitted for registration in Australia (Sethi, 2002). Toxicology studies on d-allethrin generally showed low mammalian toxicity. The IPCS evaluation in 1989 concluded that, under recommended conditions of use, the exposure of the general population to allethrins is negligible and is unlikely to present a hazard. Also, with the usual precautions, the use of allethrins is unlikely to present a hazard to those occupationally exposed to them. The WHO hazard classification of d-allethrin is: slightly hazardous. The attention of the Meeting was drawn to the recent restriction on household uses of synthetic pyrethroids in The Netherlands because of reports of neuronal effects following the exposure of neonatal mice (Schreuder, 2002). d-Allethrin is of low toxicity to birds. Toxicity testing showed that S-bioallethrin is toxic to fish and allethrin is toxic to honey-bees. It is reasonable to conclude that d-allethrin is also toxic to fish and honey-bees. The IPCS evaluation in 1989 concluded that it is unlikely that allethrins or their degradation products will attain significant levels in the environment. In spite of the high toxicity of these compounds to fish and honey bees, they are only likely to cause a problem in the case of spillage or misuse. The IPCS evaluation of 1989 was relevant to the uses at that time. It is reasonable to conclude that d-allethrin is hazardous to fish and honey-bees, while the risk will depend on their exposure. The analytical method for active ingredient (capillary GC with FID) is in process of validation by CIPAC3. This method also serves as a non-stereospecific identity test. An IR spectrum is available as an additional non-stereospecific identity test. An analytical method for isomer composition is available. The optical isomer ratios are determined by HPLC using a chiral stationary phase, while the geometric isomer ratio is determined by GLC on a non-chiral stationary phase. It
1

2003 footnote. In tests using the Buehler method, the LOAEL for chrysanthemic anhydride in d-allethrin was 100 g/kg. 2004 footnote. The limit of 10 g/kg is in accordance with the classification and labelling of skin sensitizers provided by the Globally Harmonized System of Classification and Labelling of Chemicals (United Nations, New York and Geneva, 2003, 443 pages). 2003 footnote. The analytical method for determination of d-allethrin content was successfully validated and adopted by CIPAC in June, 2003.

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has been company validated1. The method for isomer composition is recommended as the primary identity test. No special requirements for containers and packages have been identified. The proposed specification for d-allethrin TC requires the determination of d-allethrin content and measurements of total trans-isomer and total 1R-isomer contents in the active ingredient to distinguish d-trans-allethrin from other mixtures of allethrin isomers. A limit of 10 g/kg in the TC was proposed for chrysanthemic anhydride2. The existence of an FAO specification for d-trans-allethrin TC (303/2/S/3) should be noted. Recommendations The meeting recommended that the draft specification for d-allethrin TC proposed by Sumitomo Chemical Company Ltd, with amendments, should be adopted by WHO, subject to acceptable validation of analytical methods for the active ingredient and relevant impurity3. References
Arai M., Sato T. and Hagiwara A., 1985 Chronic toxicity and oncogenicity study of Pynamin Forte in rats, Daiyukai Institute of Medical Science. Sumitomo report # KT-0058. Unpublished.

Chari S., Shepler K. and Ruzo L. Sunlight photodegradation of [Alc-14C]-d-trans-allethrin in a buffered O., 1990 aqueous solution at pH 5, Pharmacology and Toxicology Research Laboratory, Project 197W. Sumitomo report # KM-01-0006. Unpublished. Dalgard D. W., Learn M., Machotka S. V., Alsaker R. D., Dalgard D. W., Rubin L. F., Bromberg N. M. and Hagen W. H., 1987 Four-week toxicity study in dogs with Pynamin Forte, Hazleton Laboratories, Sumitomo report # KT-0067. Unpublished.

Dalgard D. W., May E. R., Burns Chronic toxicity of Pynamin Forte in dogs, Hazleton Laboratories, J. M., Alsaker R. D. and Kuhlman Sumitomo report # KT-0085. Unpublished. S. M., 1989

2003 footnote. The analytical method for determination of isomer composition by HPLC using a chiral stationary phase was successfully peer validated and adopted by CIPAC in June, 2003. 2003 footnote. The analytical method for determination of chrysanthemic anhydride in d-allethrin TC was successfully peer validated and is appended to the specification. The method is not specific to chrysanthemic anhydride (other anhydrides may interfere) and it has limited sensitivity. However, it is fit for the intended purpose and is simpler and likely to be more robust than more specific and/or sensitive methods. 2003 footnote. The analytical method for determination of d-allethrin content was successfully validated and adopted by CIPAC in June, 2003. Methods for determination of the isomer ratio (identity test) and of the content of the chrysanthemic anhydride (relevant impurity) were also successfully peer validated in 2003. Validation and adoption of the methods enabled the specification to be published in 2003.

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Edwards C.A., Connaughton K., Pynamin Forte toxicity to mice by repeated dietary administration Crook D., Heywood R., Gopinath for 5 weeks, Huntingdon Research Centre Ltd. Sumitomo report # C., Gibson W. A., Majeed S. K. KT-0064. Unpublished. and Anderson A., 1986 Estigoy L., Shepler K. and Ruzo Hydrolysis of [Alc-14C]-d-trans-allethrin at pH 5, 7 and 9, Pharmacology and Toxicology Research Laboratory Project 196W. L. O., 1990 Sumitomo report # KM-01-0007. Unpublished. Fink R. and Beavers J. B., 1978a Eight-day dietary LC50 - bobwhite quail Pynamin Forte final report, Wildlife International Ltd. Sumitomo report # KT-0022, Unpublished. Fink R. and Beavers J. B., 1978b Eight-day dietary LC50 - mallard duck Pynamin Forte final report, Wildlife International Ltd., Sumitomo report # KT-0001. Unpublished. Fujita T., 2002a Fujita T., 2002b Furuta R., 2002 Furuta R., 2003a Analytical methods of isomers composition in d-allethrin. Sumitomo Chemical Co. Unpublished. Analytical method of chrysanthemic anhydride in d-allethrin. Sumitomo Chemical Co. Unpublished. d-Allethrin (no. 742). Small-scale collaborative study and analytical method. CIPAC/4276/R and CIPAC/4275/m. Analytical method for the determination of isomer ratio of d-allethrin technical material and formulations. Sumitomo Chemical Co. Unpublished. Peer validation for the analytical method of d-allethrin isomer ratio in technical material and formulations. Sumitomo Chemical Co. Unpublished.

Furuta R, 2003b

Hiromori T., Yamada H., Kato T., Acute oral toxicity study of Pynamin Forte in rats, Sumitomo Kohda A., Misaki Y. and Okuno Chemical Co., Ltd. Sumitomo report # KT-0079. Unpublished. Y., 1989a Hiromori T., Yamada H., Kato T., Acute dermal toxicity study of Pynamin Forte in rabbits, Sumitomo Kohda A., Sako H. and Okuno Chemical Co., Ltd. Sumitomo report # KT-0080. Unpublished. Y., 1989b Hoberman A. M., 1989a Reproductive effects of Pynamin Forte administered orally in feed to CRL;COB CD (SD)BR rats for two generations, Argus Research Laboratories, Inc. Sumitomo report # KT-0087. Unpublished. Teratology study in rats with Pynamin Forte, Argus Research Laboratories, Inc. Sumitomo report # KT-0094. Unpublished. Teratology study in rabbits with Pynamin Forte, Argus Research Laboratories, Inc. Sumitomo report # KT-0097. Unpublished. Determination of boiling point/boiling range of Pynamin Forte, Guideline 40 CFR 158, Project HLA 6001-309, Hazleton Laboratories America, Inc. Report KP-91-0053. Unpublished. Allethrins - allethrin, d-allethrin, bioallethrin, S-bioallethrin. Environmental Health Criteria, 87. WHO, Geneva. 90-Day subacute toxicity study of Pynamin Forte on rats, Sumitomo Chemical Co., Ltd. Sumitomo report # KT-0006. Unpublished.

Hoberman A. M., 1989b Hoberman A. M., 1989c Hoffman M. J., 1989

IPCS, 1989 Kadota T., 1977

Kawaguchi S., Yamada H., Kato Acute inhalation toxicity study of Pynamin Forte in rats, Sumitomo T., Kohda A. and Ito S., 1989 Chemical Co., Ltd. Sumitomo report # KT-0082. Unpublished.

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Kawaguchi S., Kato T., Yoshioka Three-month inhalation toxicity study of Pynamin Forte in rats, K. and Uwagawa S., 1993 Sumitomo Chemical Co., Ltd. Sumitomo report # KT-0123. Unpublished. Kogiso S., Yamada H., Yoshitake Reverse mutation test of Pynamin Forte in Salmonella typhimurium A. and Yamada F., 1989a and Escherichia coli, Sumitomo Chemical Co., Ltd. Sumitomo report # KT-0083. Unpublished. Kogiso S., Yamada H., Yoshitake In vitro unscheduled DNA synthesis (UDS) assay of Pynamin Forte A., Kawamoto M. and Hara M., in rat hepatocytes, Sumitomo Chemical Co., Ltd. Sumitomo report 1989b # KT-0089. Unpublished. Kogiso S., Yamada H., Yoshitake In vitro chromosomal aberration test of Pynamin Forte in Chinese A., Yamamoto K. and Hara M., hamster ovary cells (CHO-KI), Sumitomo Chemical Co., Ltd. 1989c Sumitomo report # KT-0088. Unpublished. Mauck W. L., Olson L.E. and Marking L.L., 1976 Mayfield R., Gopinath C., Crook D., Offer J., Anderson A. and Gibson W. A., 1989 Mukumoto M., 2003a Toxicity of natural pyrethroids and five pyrethroids to fish, Archives of Environmental Contamination and Toxicology, 4, 18-29. Published report. Pynamin Forte potential tumorigenic effects in prolonged dietary administration to mice, Huntingdon Research Centre Ltd. Sumitomo report # KT-0086. Unpublished. Analytical method for the determination of chrysanthemic anhydride in d-allethrin technical material. Sumitomo Chemical Co., Ltd. Unpublished. Peer validation of the analytical method of chrysanthemic anhydride in d-allethrin technical material. Sumitomo Chemical Co., Ltd. Unpublished. Primary eye and skin irritation tests with Pynamin Forte in rabbits, Sumitomo Chemical Co., Ltd. Sumitomo report # KT-0078. Unpublished. Skin sensitization test with Pynamin Forte in guinea pigs, Sumitomo Chemical Co., Ltd. Sumitomo report # KT-0081. Unpublished.

Mukumoto M., 2003b

Nakanishi T., Yamada H., Kato T. and Kohda A., 1988 Nakanishi T., Yamada H., Kato T. and Kohda A., 1989

Saito S., Yamada H., Matsuda Water solubility of Pynamin Forte, Sumitomo Chemical Co., T. and Tanoue A., 1989a Ltd. Sumitomo report # KP-90-0051. Unpublished.
Saito S., Yamada H., Matsuda T. Partition coefficient (n-octanol/water) of Pynamin Forte, Sumitomo and Tanoue A., 1989b Chemical Co., Ltd. Sumitomo report # KP-90-0052. Unpublished. Schreuder R., 2002 Personal communication to the JMPS, relating to two unpublished documents: (i) minutes of the European Commissions Special Meeting of the Working Group Pesticides - Evaluation, held 19 June 2002, to discuss questions related to developmental neurotoxicity (incorporating comments of the Swedish delegation, 20 June, 2002). (ii) Synthetic pyrethroids/28-08-02, letter from the Netherlands government to the European Commission, 2002.

Semann T. M., Pesselman R. L. Vapor pressure determination of Pynamin Forte, Hazleton and Hoffman M. J., 1989 Laboratories America, Inc., project HLA 6001-269. Sumitomo report # KP-91-0056. Unpublished. Sethi P., 2002 WHO specifications for d-allethrin and d-phenothrin, letter 30 May 2002, National Registration Authority, Australia.

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