LC-40B X3 CL: Instruction Manual

228-97198B
Sep. 2022
Solvent Delivery Module for Shimadzu

Liquid Chromatograph
LC-40B X3 CL
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
This page is intentionally left blank.
Introduction
Read this Instruction Manual thoroughly before using
the product.
Thank you for purchasing this product.
This instruction manual describes the basic operation, and accessories and options for this
product. Read this manual thoroughly before using the product and operate the product
in accordance with the instructions in this manual.
The following instruction manuals are included with the product in booklet form or in
PDF format. The PDF documentation is on the instruction manual CD-ROM (Part No.
228-97193-41).
Document Name Document No. Description

Instruction Manual (PDF) 228-97198 This instruction manual.
This manual provides details on how to use
the system:
System Guide (PDF) 228-97194 system performance optimization, analysis
procedure, troubleshooting, validation,
installation, etc.
Safety Guideline Describes the precaution instructions to
228-97195
(Booklet/PDF) ensure safe operation.
Read "Safety Guideline" thoroughly before using the product.

"Safety Guideline" describes the information about the warranty, after-sales service,
safety instructions and precautions to ensure safe operation of the instrument. Keep this
manual for future reference.
Important
• If the user or usage location changes, ensure that this Instruction Manual is always
kept together with the product.
• If this manual or a product warning label is lost or damaged, immediately contact
your Shimadzu representative to request a replacement.
• To ensure safe operation, read the accompanying booklet "Safety Guideline"
before using the product.
• To ensure safe operation, contact your Shimadzu representative if product
installation, adjustment, re-installation (after the product is moved), or repair is
required.
ã 2020-2022 Shimadzu Corporation. All rights reserved.

Original version is approved in English.
LC-40B X3 CL i
Introduction
Notice
• Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
• All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission in writing from Shimadzu Corporation.
• Nexera and LabSolutions are trademarks of Shimadzu Corporation.
Windows is a registered trademark of Microsoft Corporation in the United States and/or
other countries.
Third-party trademarks and trade names may be used in this publication to refer to
either the entities or their products/services, whether or not they are used with
trademark symbol "TM" or "®".
Indications Used in This Manual

Precaution symbols are indicated using the following conventions:
Indication Meaning
Indicates a potentially hazardous situation which, if not

avoided, could result in serious injury or possibly death.
Indicates a potentially hazardous situation which, if not

avoided, may result in minor to moderate injury or equipment
damage.
Emphasizes additional information that is provided to ensure
the proper use of this product.
The following symbols are used in this manual:
Indication Meaning
Indicates an action that must not be performed.
Indicates an action that must be performed.
Indicates information provided to improve product performance.
Indicates the location of related reference information.
ii LC-40B X3 CL
Electromagnetic Compatibility
Electromagnetic Compatibility
Descriptions in this section apply only to the following models:
• 228-65078-55 LC-40B X3 CL
This product complies with European standard EN61326, class B for electromagnetic
interference (Emissions) and industrial electromagnetic environment (Immunity).
n EN55011 Emissions (Electromagnetic Interference)
This is a class B product. When this product causes an electromagnetic disturbance to

devices being used near this product, create an appropriate distance between those
devices and this product in order to eliminate the disturbance.
Operating Nexera CL System

n Intended Use
This system is designed to perform qualitative and quantitative analysis of target

compounds in a sample matrix and can be used as a liquid delivery unit of the liquid
chromatograph for general in vitro diagnostic applications. However, only personnel who
have received appropriate training on use of the system can use it for these purposes.
n Calibration
A calibration curve should be generated for each analyte in appropriate methods. At

least four out of six non-zero standards should meet the appropriate criteria, including
the calibration standard lower than the assumed LOQ and the calibration standard at
the highest concentration.
n Quality Control
Implement quality control of the instrument by routinely measuring at least one each of
four types of quality-control samples (a sample with a normal concentration level, a
sample with a concentration level higher than normal as well as a sample lower than
normal, and a blank sample). Check the selectivity/specificity in the matrix used for the
actual sample, the accuracy, correctness, recovery rate, dynamic range, linearity, and lower
limit of quantitation.
Based on these assessment results, check that the results are within the permissible range.
If the assessment results are out of range, the measured data may be invalid, so do not
use analysis results obtained from the instrument in question until it can be confirmed
that it is functioning normally. When, for example, analyzing samples that contain
complex sample matrices such as serum, plasma or urine, it may be possible to obtain
stabilized data by using an appropriate pre-treatment or an internal standard.
LC-40B X3 CL iii
Contents
Introduction Indications Used in This Manual ......................... ii
Electromagnetic Compatibility ............................ iii
Operating Nexera CL System .............................. iii
1 Overview 1.1 Features ............................................................. 1

1.2 Component Parts ................................................. 2
1.3 Optional Parts ..................................................... 2
Parts Identification 6
2 and Function
2.1 Front ..................................................................
2.2 Behind Front Cover ............................................. 7

2.3 Back ................................................................... 9
2.4 Right Side and Bottom ...................................... 10
2.5 Name and Functions of the Operation Panel ....... 11
2.5.1 Display Area ................................................... 13
2.5.2 Operation Area ............................................... 15
3 Operation 3.1 Settings for the Basic Operation ......................... 17

3.1.1 Prior to Key Operation ........................................ 17
3.1.2 Setting Flow Rate ............................................. 17

3.1.3 Setting Compressibility ........................................ 18
3.1.4 Setting Maximum Pressure Limit .............................. 18
3.1.5 Setting Minimum Pressure Limit .............................. 19
3.2 Operation ........................................................ 20

3.2.1 Preparation before Operation ................................. 20
3.2.2 Operation in Constant Flow Delivery Mode .................. 21
3.3 Types of Screens ................................................ 22

3.4 Monitoring Screens ............................................ 23
3.5 Auxiliary Functions Screen (FUNCTION) ................ 27
3.5.1 Auxiliary Functions (FUNCTION) List .......................... 27
3.5.2 [PARAMETER] Setting Group .................................. 29
3.5.3 [CONTROL] Setting Group .................................... 34
iv LC-40B X3 CL
Contents
3.5.4 [SYSTEM] Setting Group ...................................... 35

3.5.5 [UTILITY] Setting Group ....................................... 39
3.5.6 [FILE] Operation Group ........................................ 41
3.6 VP Functions Screen ........................................... 42

3.6.1 List of VP Functions .......................................... 42
3.6.2 Product Information Group (PRODUCT INFO) ................ 44
3.6.3 Maintenance Information Group (MAINTENANCE) ........... 44
3.6.4 Validation Support Information Group (VALIDATION) ........ 48
3.6.5 Calibration Support Information Group (CALIBRATION) ...... 55
3.7 Creating Time Program ...................................... 63

3.7.1 Time Program Command List ................................. 63
3.7.2 Time Program Setting Screen ................................. 64
3.7.3 Creating a High Pressure Gradient Program ................. 65

3.7.4 Deleting a Step ............................................... 67
3.7.5 Starting and Stopping a Time Program ...................... 68
3.7.6 Commands Used for Time Programs Only ................... 68
3.8 Connection with the System Controller or
Workstation ...................................................... 69
3.8.1 Setting the Instrument ........................................ 69
3.8.2 Basic Parameters .............................................. 69
3.8.3 Notes on Operation ........................................... 70
3.9 Connection to External Input/Output Terminals

......................................................................... 70
4 Maintenance 4.1 Periodic Inspection and Maintenance .................. 71

4.1.1 Prior to Inspection and Maintenance ......................... 72
4.1.2 List of Periodic Inspection and Maintenance ................. 73
4.1.3 Check after Inspection and Maintenance .................... 74

4.2 Removing/Installing the Pump Heads ................... 76
4.3 Replacing the Plunger Seal ................................ 82
4.3.1 Replacing the Plunger Seal and Backup Ring ................ 83
4.3.2 Resetting the Delivery Amount ............................... 90

4.3.3 Check after Replacement ..................................... 90
4.4 Cleaning/ Replacing the Plunger and
Diaphragm ........................................................ 91
LC-40B X3 CL v
Contents
4.4.1 Removing the Pump Head and the Head Holder ........... 91
4.4.2 Cleaning and Replacement of the Plunger and the
Diaphragm ..................................................... 93
4.5 Rinsing the Check Valve .................................... 97

4.6 Performing an Ultrasonic Cleaning of the
Check Valve ...................................................... 99
4.7 Replacing the Check Valve ............................... 101
4.8 Inspecting the Line Filter ................................. 102
4.9 Replacing the Line Filter .................................. 103
4.10 Cleaning/Replacing the Suction Filter ................. 104
4.11 Replacing the Drain Valve ................................ 107
4.12 Cleaning of the Leak Tray ................................ 108
4.13 Cleaning the Exterior ....................................... 108
4.13.1 Usual cleaning ............................................... 108
4.13.2 Decontamination cleaning .................................. 109
4.14 Cleaning of the Automatic Rinsing Kit .............. 110
Technical 111
5 Information
5.1 Specifications ...................................................
5.1.1 Specifications ............................................... 111

5.1.2 High-Pressure Gradient System .............................. 112
5.1.3 Other Specifications ......................................... 112

5.2 Maintenance Parts ........................................... 115
5.2.1 LC-40B X3 ................................................... 115
5.2.2 Maintenance Kit ............................................. 116
Index ......................................................... 117
vi LC-40B X3 CL
1 Overview
This instrument is a liquid delivery unit for liquid chromatography using a parallel double
plunger reciprocating-type pump developed to improve the precision and sensitivity of 1
analysis.
By pumping alternately from two pump heads arranged in parallel, it can perform
continuous pumping. The stepping motor's torque is transmitted to the cam, causing the
plungers to perform a piston motion.
1.1 Features
n Low Flow Pulsation and Pulsation Period Enable Precise Delivery
By reducing the discharge volume per plunger stroke to the microvolume level (10 µL),
and applying high-speed drive, the flow pulsation and pulsation period have been
reduced to levels significantly lower than those for other instruments.
n HIgh-pressure Flow is Available
LC-40B X3 CL enables ultra high pressure solvent delivery up to 130 MPa, supporting ultra
fast high separation analysis in combination with Shim-pack XR-ODS III or other ultra fast
high separation columns.
n A single unit of this instrument enables you to perform high pressure gradient
LC-40B X3 CL is equipped with two pump units, with maximum pressure resistances of
130 MPa enabling highly accurate high pressure gradient with a small delay.
n Automatic Plunger Rinsing
An automatic rinsing kit is provided as standard. It can rinse the plunger in the back of
the plunger seal. When high-salt-concentration buffer solutions are used, in particular, it
prevents premature seal failure due to crystallization, extending the service life of plunger
seals.
LC-40B X3 CL 1
1 Overview
1.2 Component Parts

This instrument consists of the standard parts listed below. Check the parts against this
list after unpacking.
No. Part Name Part No. Q'ty Remark

Main Unit
- - 1
LC-40B X3 CL
- Safety Guideline (Booklet)*1 228-97195 1
Instruction manual
- 228-97193-41 1
(CD-ROM)
- Bottle, 250 mL 228-74372 1
1 Suction Filter Assy 228-45708-91 Including a tube
Not used in CL
2 REMOTE CABLE ASSY 228-28253-91 1
model.
3 DRAIN TUBE SI (L1000) 228-25162-03 1
STOP JOINT ASSY,B,D 228-46054-91 1
4
SYRINGE TUBE ASSY,B,D 228-46055-91 1
SYRINGE,SS-20ESZ 228-66048 1
5
NEEDLE ASSY,FOR SYRINGE 228-18216-91 1
6 CLAMP,M9120 046-05522-02 1
7 DRAIN ADAPTER 228-42204 1
8 STANDARD OUT DRAIN 228-42205 1
9 CTO OUT DRAIN 228-42206 1
10 STRAIGHT JOINT 228-28163 1
11 FITTING,L-TYPE 1253 035-61561-12 1
12 CABLE,PUMP CJ19-LITE 228-70156-41 1 Not use in CL model.
13 SF Nut,1/8 228-56687-42 3 Used for connecting
the rinse path of the
14 SF Ferrule,1/8 228-56687-43 3 auto sampler.
Attach to the rinse
15 CAP ASSY,40B X3 WASHING 228-57084-41 1
solution bottle.
*1 It contains cautions for use regarding the instrument.
1.3 Optional Parts

Optional units available for this instrument are listed below.
2 LC-40B X3 CL
1.3 Optional Parts
For information about other optional units listed below, contact your Shimadzu
representative.
Option Part No. Features

1
Gradient Mixer for
High-Pressure Gradient "Gradient Mixer for High-Pressure Gradient Analysis" P.3
Analysis
The rinsing pump kit is intended for
continuously rinsing the back part of the plunger
RINSING PUMP XR 228-39625-42
seal with rinsing solution. The rinsing volume
can be selected.
Kit containing the necessary tools for
Tool Kit 228-57647-43
preparation, inspection, and maintenance.
Plumbing for the pump inlet. ID 0.3 mm
SUS Pipe (0.3´500) 228-53184-51
´1000 mm, pre-bent
SUS Pipe HP (0.3´1,000) 228-53184-96 Plumbing for the pump outlet. ID 0.3 mm´1000
The maximum available pressure is 44MPa.

When using a non-polar organic solvent such as
Plunger Seal GFP 228-52711-94
hexane or a solvent of alkyl sulfonic acid with
TFA, use the standard plunger seal.
Suction filter with tube and fittings for
SUCTION FILTER ASSY 228-61905-49
degassing unit.
A set of consumables and tubing parts for
LC-40B X3 CL. This kit is also used for the
Maintenance Kit for instrument.
228-53265-46
LC-40B X3
"Maintenance Kit for LC-40B X3 CL(Parts
No. 228-53265-46)" P.116
n Gradient Mixer for High-Pressure Gradient Analysis
The following gradient mixers for high-pressure gradient analysis are available for high
performance liquid chromatographs.
Part Name Part No.

(w/ mixer recognition device) 228-72652-41
MIXER,MR 20 µL
(w/o mixer recognition device) 228-45209-41
MIXER,MR 40 µL
MIXER,MR 100 µL
MIXER,MR 180 µL Ⅱ
Mixers described above are packaged in a set
Part Name Part No.

Set of Two Mixers MR 40 µL and MR 180 µLⅡ 228-45292-41
LC-40B X3 CL 3
1 Overview
Part Name Part No.

Set of Two Mixers MR 100 µL and MR 180 µLⅡ 228-45292-42
Set of Three Mixers MR 40 µL, MR 100 µL and MR 180 µLⅡ 228-45292-43
Recommended Mixers
The table below lists the mixers with the smallest volume among mixers that can be used
in individual combinations of detectors and mobile phases.
Mobile Phase
Water (Including
Solvent Containing Solvent Containing Buffer Solution),
Trifluoroacetic Formic Acid, Organic Solvent,
Acid (TFA) Acetic Acid, etc. or Its Solvent
Mixture
Photo Diode Array
MR 180 µL MR 180 µL MR 180 µL
Detector
Detector
UV Detector MR 180 µL MR 100 µL MR 40 µL
LCMS, LCMSMS - MR 20 µL MR 20 µL
Using a mixer with a volume smaller than the recommendation shown above may cause
excessive fluctuation of the baseline.
When connecting multiple detectors, use a mixer with the largest volume among the
applicable mixers. For example, if using a Photo Diode Array and an LC/MS, the MR 180
µLⅡmixer is recommended.
A larger than recommended mixer can be used to improve mixing performance, but will
also increase the gradient delay volume.
4 LC-40B X3 CL
1.3 Optional Parts
n Automatic Rinsing Kit
The plunger can be rinsed with the automatic rinsing kit at the back part of the plunger
seal. LC-40B X3 CL has the automatic rinsing kit as standard.
See the table below for the necessity of the automatic rinsing kit and rinsing solution.
1
Mobile Phase Necessity Recommended Rinsing Solution

Buffer Solution Necessary 10 % 2-propanol water
Normal-phase solvent such as
Not necessary -
hexane
Other Solutions Necessary 10 % 2-propanol water
Necessary: The rinsing kit is necessary.

Not necessary: The rinsing kit is not necessary.
• For an application system, refer to the instruction manual of the system.

• If the rinse solution is dirty, replace with new solution. It getting significantly
dirty, replace with new solution once a day. Even if it does not appear dirty,
replace with new solution once a week.
LC-40B X3 CL 5
2 Parts Identification and Function
2.1 Front
No. Name Description

Configure or operate the instrument with the operation keys.
1 Operation Panel Touching the center part on the operation panel displays the
operation keys.
2 Front Cover This is the cover for the flow line.
Switches ON/OFF the power.
Pressing the switch turns on the power and pressing it again
3 (Power button) turns off the power.
The switch is enabled only when the main power switch on
the back is on.
6 LC-40B X3 CL
2.2 Behind Front Cover
2.2 Behind Front Cover

For LC-40B X3 CL, pump A is on the lower side, and pump B is on the upper side, when
viewed from the front.
The following takes pump A as an example to show the names of the parts.

1 Check Valve OUT*1 A check valve on the outlet side.
It has the rinsing flow path of the plunger inside. The
2 Head Holder*1
pump head is attached to this part.
It is used to replace mobile phase solvent or remove
3 Drain Valve air from the flow path. It has an internal pressure
sensor.
4 Pump Head*1 The plunger inside it reciprocates to deliver solvent.
5 Check Valve IN*1 A check valve on the inlet side.
6 Pump Inlet A connection port of the tube from the suction filter.
A connection port of the tube connected to the
7 Pump Outlet
autosampler/manual injector.
A column protection filter for removing contaminants
8 Line Filter
from mobile phase solvent.
LC-40B X3 CL 7
It detects liquid leaks.

If the leak sensor detects a leak, delivery automatically
stops, causing an alarm and message on the display.
9 Leak Sensor
A pump to deliver rinse solvent for the plunger seal

10 Seal Rinse Pump
and the plunger.
11 Degassing Unit A device to degas mobile phase.
*1 This is common to the right and left pump heads.
8 LC-40B X3 CL
2.3 Back
2.3 Back
No.*1 Name Description

1A/1B [REMOTE] Connector A connector for connecting the system controller.
External Input/Output
2A Connect to external equipment.
Terminals
To input the pressure signal from the external
3A/3B [DGU PRESS IN] Connector
degassing unit.
A connector for connecting rinsing pump or degassing
4A [DGU/SV] Connector
unit.
4B [DGU] Connector Used for connecting two degassing units.
5A/5B [PC] Connector A connector for service personnel. Normally unused.
6 Power Cord Connector Connect the power cord.
It turns ON/OFF the power to the instrument. Normally
7 Main Power Switch
keep it on.*2
*1 The letter "A" indicates the connectors for pump A and the letter "B" the connectors for pump B.
*2 Normally use (power button) of the operation panel or system controller to turn ON/OFF the
power. If the system controller is connected and the main power switch is on, the user can use
(power button) of the system controller to turn ON/OFF the power from the front of the instrument.
If the instrument is not used for a long time, turn off the main power switch. Before turning off
the main power switch, be sure to turn OFF the power using the power button.
"2.5 Name and Functions of the Operation Panel" P.11
LC-40B X3 CL 9
2.4 Right Side and Bottom

1 Mixer Mounting Holes Used to install mixer and column holder.
To prevent damage during transportation.
2 Shipping Screws
Remove before installation.
3 Leakage Drain Outlet Connect the provided drain tubing.
10 LC-40B X3 CL
2.5 Name and Functions of the Operation Panel

This instrument is controlled through the keypad of the operation area. The display area
allows verification of the instrument status. The operation area has two input modes:
[Arrow key input mode] to enable screen transfer, and [Numerical key input mode] to
enable value input.
Pressing [Enter] switches between these input modes.
When turning off the main power switch on the back of the instrument after 2
changing the parameters via panel operation, be sure to turn OFF the power
with the power button at the front of the instrument, and turn off the main
power switch. Otherwise, some of the changed parameters may return to their
original values.

1 Display Area Displays various screens and settings.
2 Link LED Illuminates when controlled by the system controller.
• Green: analysis ready
• Red: error
• Blue: analysis in progress
3 Status LED
• Yellow: preparation for analysis in progress (The indicator
normally lights in yellow while no solvent is delivered.)
• Orange: sleep
LC-40B X3 CL 11
Switches ON/OFF the power.

• To turn the power ON:
Press and hold the power button for at least 3 seconds.
• To turn the power OFF:
(Power Holding down the power button 3 seconds or more
4 displays the confirmation screen as shown below. Holding
Button)*1 it down again 1 second turns off the power.
Press [CE] to cancel the operation.
Delivery start/stop, gradient concentration setting,

5 Direct Key
timeprogram start and purge can be performed directly.
*1 If CBM-40 CL is used, pressing the power button of the pump turns off the power of the entire
system.
Note, however, that the following will apply in the case of a system with multiple pumps.
- When using CBM-40 CL: The system power can be turned off using the power button of the
pump with the lowest optical link number.
12 LC-40B X3 CL
2.5.1 Display Area
Use the keys on the front to operate or configure the instrument. Touch the key to make
the status LED brighter, accept input when you release the key.
n In high pressure gradient mode ([PUMP CONFIG (SYS)] = 2)
"Setting the system parameter《PUMP CONFIG (SYS)》" P.37
No. Name Function

1 T.flow Displays the total flow rate (mL/min) of pumps A and B.
2 BCONC Displays the concentration (%) of the mobile phase for pump B.
Displays pressure values of pumps A and B measured by the
3, 4 pressure A/B pressure sensor in the unit of pressure specified by the auxiliary
function [PRESSURE UNIT].
5 PUMP Highlighted during pump operation.
Highlighted in the high-pressure gradient mode or blinks in the
6 CONC
high-speed LC mode.
Highlighted during execution of a time program or in the
7 RUN
high-speed LC mode.
8 PURGE Highlighted during purging.
LC-40B X3 CL 13
n In isocratic mode ([PUMP CONFIG (SYS)] = 1)
No. Name Function

1, 2 flow A/B Displays the set flow rate (mL/min) of pumps A and B.
Displays pressure values of pumps A and B measured by the
3, 4 pressure A/B pressure sensor in the unit of pressure specified by the auxiliary
function [PRESSURE UNIT].
5 PUMP Highlighted during pump operation.
Highlighted in the high-pressure gradient mode or blinks in the
6 CONC
high-speed LC mode.
Highlighted during execution of a time program or in the
7 RUN
high-speed LC mode.
8 PURGE Highlighted during purging.
14 LC-40B X3 CL
2.5.2 Operation Area
Use the keys on the front to operate or configure the instrument.
No. Key Name Function

1 [PUMP] Pump Key Starts or stops the pump.
Concentration
2 [CONC] Sets liquid concentrations in gradient analysis.
Key
Starts or stops a time program. (Key operation is
3 [RUN] Run Key
ignored if a time program is not configured.)
Starts or stops purging. Purging stops automatically 3
minutes after it begins.
4 [PURGE] Purge Key
[PUMP] can also be used to stop purging. Purging time
can be set with the [PURGE TIME] auxiliary function.
This key is used for the following;
• To initialize the screen
5 [CE] Clear Key • To clear the values input up to that time while
entering the values.
• To clear error message and cancel alarms.
Use in the following cases.
• To delete input values.
6 [del] Delete Key
• To delete a line of the displayed time program while
creating it.
7 [Enter] Enter Key Confirms input values of the setting items
[↑][→]
8 Arrow Keys Moves the cursor or switches the screen
[↓][←]
9 [·] to [9] Numeric Keys Inputs values for the setting items.
10 [ - ] Minus Key No use
LC-40B X3 CL 15
Regarding the acceptable part of the arrow keys on the touch panel.
When operating the arrow keys, it
responds by touching the part including
the upper and lower range of each key
indication on the operation panel.
No. Description
1 Acceptable part of [↑] key input.
2 Acceptable part of [→] key input.
3 Acceptable part of [↓] key input.
4 Acceptable part of [←] key input.
16 LC-40B X3 CL
3 Operation
3.1 Settings for the Basic Operation
Before operating this instrument, it is necessary to set the flow rate and the pressure
limit to protect flow line parts such as columns.
See the product specifications before use.
"5.1.1 Specifications" P.111
3.1.1 Prior to Key Operation
Touching the central part of the operation panel displays the operation keys that the 3
user can operate.
3.1.2 Setting Flow Rate
The following is the basic procedure for setting a flow rate [FLOW].
1 Displays the initial screen (the screen

that is displayed when the power is
turned ON).
If the initial screen is not displayed, press
[CE] to display the initial screen.
2 Press [Enter].
This starts the cursor blinking at the input position and enables input of the total flow
rate [T.flow].
3 Input the total flow rate with the numeric keypad and press [Enter].
This confirms the total flow rate value [T.flow] and then enables input of concentration
of the mobile phase for pump B [BCONC].
4 Input concentration of the mobile phase for pump B with the numeric keypad
and press [Enter].
This confirms the concentration value of pump B [BCONC] and then displays the initial
screen.
To cancel the input value, press [CE].

[BCONC] can also be set by using the [CONC] key.
LC-40B X3 CL 17
3 Operation
3.1.3 Setting Compressibility
The following explains how to set the compressibility of the mobile phase in pump A.
Set the compressibility of the mobile phase in pump B in the same way.
1 Press [→] twice on the initial screen.

The FUNCTION setting group is displayed.
2 Move the cursor to [PARAMETER], and

press [→].
The PARAMETER setting group is displayed.
3 Press [↓] repeatedly until [COMP A]

(compressibility correction for pump A)
is displayed in the screen and press
[Enter].
The cursor blinks at the input position
indicating that the compressibility [COMP] can be input.
4 Input the compressibility [COMP A] for the solvent to be used (0.45 for water)
with the numeric keypad.
"Setting the compressibility of solvent《COMP A/B》" P.31
3.1.4 Setting Maximum Pressure Limit
The maximum pressure limit is the pressure

in the flow line that may not be exceeded.
The following explains how to set the
maximum pressure limit of pump A. Set
the maximum pressure limit of pump B in the same way.
If the pressure exceeds the maximum
pressure limit, delivery operation
automatically stops, causing an alarm to
go off and a message to be displayed.
n Setting Procedure
The following explains how to set the pump A maximum pressure [P.MAX] to 15.0 MPa.
1 Press [→] three times on the initial

screen.
18 LC-40B X3 CL
3.1 Settings for the Basic Operation
2 Press [Enter].
The cursor blinks at the input position indicating that [P.MAX A] can be input.
3 Press [1], [5], [ . ], [0], and [Enter].

The maximum pressure is set to 15.0 MPa.
The allowable maximum pressure is 130 MPa for LC-40B X3 CL. When connecting
the instrument with other equipment, set the maximum pressure to the lowest
allowable maximum pressure of the equipment.
"Setting the maximum pressure limit during pumping《P.MAX A/B》" P.30
3
3.1.5 Setting Minimum Pressure Limit
The purpose of the minimum pressure limit

is to prevent a pressure drop which can
occur as a result of the following
situations:
• When the mobile phase runs out, air will be pumped through the flow lines resulting
in a pressure drop.
• When a leak occurs in the flow lines, a pressure drop may be observed.
The following explains how to set the minimum pressure limit of pump A. Set the
minimum pressure limit of pump B in the same way.
If the pressure is still lower than the
minimum pressure limit 1 minute after
delivery operation starts, the delivery
operation stops automatically. Then the
error message will be displayed with the
alarm sound.
Then the error message will be displayed with the alarm sound.
n Setting example
The following explains how to set the pump A minimum pressure [P.MIN A] to 2.0 MPa.
1 Press [→] three times on the initial screen.

2 Press [↓] twice and on the [P.MIN A]

screen, press [Enter].
The cursor blinks at the input position
indicating that [P.MIN A] can be input.
LC-40B X3 CL 19
3 Operation
3 Press [2], [ . ], [0] and [Enter].

The minimum pressure is set to 2.0 MPa.
If [P.MIN] is set to "0", solvent delivery will not stop and an alarm will not
sound even if the pressure has dropped.
"Setting the minimum pressure during delivery《P.MIN A/B》" P.30
3.2 Operation
3.2.1 Preparation before Operation
1 Pour the mobile phase into the reservoir bottle.
2 Place a suction filter into the reservoir bottle.
3 Connect the tubing provided to the pump outlet.
4 Put the end of the drain tubing into the waste container.
5 Turn the drain valve knob

90°counterclockwise to open the drain
valve.
20 LC-40B X3 CL
3.2 Operation
6 Press [PURGE], and then [ENTER].
During delivery, first press [pump]

and [ENTER] to stop the delivery,
then press [purge] and [ENTER].
IF the drain valve knob is turned more than 180°, any mobile phase that drains
out may contain air bubbles. This is normal.
3.2.2 Operation in Constant Flow Delivery Mode
This instrument supports "constant flow delivery mode" in which the instrument operates
at a specified flow rate. 3
1 Turn the drain valve knob clockwise as far as possible to close the drain valve.
2 Press [CE].
The initial screen is displayed.
3 Press [Enter] and set the desired flow

rate and the solvent B concentration.
4 Press [PUMP], and then [ENTER].

[PUMP] in the display panel is highlighted
and pumps A and B start operation at the
same time.
5 Check that the pump outlet pressure is increasing on the pressure indicating
display.
6 To stop operation, press [PUMP], and then [ENTER].

[PUMP] on the display stops being highlighted and the pump stops operation.
Close the drain valve when not draining through the drain path.
If the drain valve is left opened, a pressure differentia may cause the mobile
phase solvent to flow out of the drain outlet.
LC-40B X3 CL 21
3 Operation
3.3 Types of Screens

Turning ON the power displays the initial screen.
By pressing the [↓] and [→] arrows on the initial screen, the screen can be switched
from the initial screen to one of three screens below:
Displays Screen Description

View the status of the instrument.
Monitor screen
"3.4 Monitoring Screens" P.23
Configure various parameter settings and perform operations.

Auxiliary functions screen
"3.5 Auxiliary Functions Screen (FUNCTION)" P.27
Supports the validation of the instrument with functions for

VP function screen viewing and checking instrument information.
"3.6 VP Functions Screen" P.42
Pressing [→] on the initial screen displays the screen to select the auxiliary functions
screen [FUNCTION] or VP functions screen [VP]. Move the cursor to the desired option
with [↓] or [↑] and press [→] to display the screen. Press [←] to return to the previous
screen. And press[CE] to return to the initial screen.
22 LC-40B X3 CL
3.4 Monitoring Screens

This section describes the monitoring
screens.
Press [↓] on the initial screen to show the
various types of information.
Name Description Reference

MONITOR TIME To show ｔhe elapsed time while a time program runs. P.23
MONITOR FLOW To show the actual flow rate during delivery. P.23
MONITOR ID/SV Not used in CL model. -
To show the Auto-Diagnostics result when 3
Auto-Diagnostics is on. The delivery status of the pump is
shown when Auto-Diagnostics is off.
"Turning on/off Auto-Diagnostics《AUTO
CONDITION A/B DIAGNOSTICS》" P.62 P.24
Auto-Diagnostics can cause a warning when a

delivery failure is detected.
DEGAS PRES A/B To show the vacuum pressure of the degassing unit. P.25
To show the status of the connection between the
PC/WS*1 P.26
instrument and the PC/WS.
*1 Shown only when CBM-40 CL is connected.
n Displaying the time that elapsed after a program was started《MONITOR TIME》
While a time program is running, the

panel displays the time that elapsed after
the program was started.
The time is displayed only when

PUMP CONFIG (SYS) is set to "2"
(high pressure gradient mode).
n Displaying the actual flow rate during delivery《MONITOR FLOW》
On the right column of the screen, the

flow rate actually delivered is displayed in
real time while the flow rate is gradually
increased with the [FLOW SLOPE] function.
"Setting the time to reach the set
flow rate after the start of delivery
《FLOW SLOPE A/B》"
LC-40B X3 CL 23
3 Operation
n Displaying the pump ID and the port of the flow path switching valve connected to
the instrument during remote control《MONITOR ID/SV》
Not used in CL model.

There are no flow path switching valves available for the CL model.
n Displays of Condition《CONDITION A/B》
The screen displays a delivery status

Auto-Diagnostics result during analysis
when the Auto-Diagnostics function is on.
The first row shows the diagnosis result of
pump A and the second row the diagnosis
result of pump B.
Display Auto-Diagnostics Status

The delivery status is not being
judged. This is displayed while
Diag Off
delivery is stopped or when not
being analyzed etc.
Judgment of the delivery status is

Diag Monitoring
in progress.
Diag NotDetected Delivery failure was not detected.
Diag Detected Delivery failure was detected.
Auto-Diagnostics issues a
warning if a pulse exceeds a
specific threshold.
Note that very slight pressure
variation may not be detected.
The table shows a guideline of the conditions under which the Auto-Diagnostics function
can correctly detect a delivery failure.
Item Specification
0.1 to 2 mL/min
Flow Rate
* Pumps should satisfy the above flow rate range.
Delivery Pressure 5 to 130 MPa
24 LC-40B X3 CL
Item Specification
Pumping Modes Isocratic, two-solvent high-pressure gradient
The Auto-Diagnostics function may not work in case of a significant pressure

decrease, such as when a large bubble enters the pump head. For this reason,
set the lower pressure limit P.MIN when using the function.
When Auto-Diagnostics is off, change in

the current pump pressure is detected and
a delivery status is displayed. The first row
shows the pump A status and the second
row the pump B status.
Display Delivery Status 3

Press Stable Delivery is stable.
The pressure of the pump head that is on the left when viewed
Press Dec Left
from the front is decreasing during discharge.
The pressure of the pump head that is on the right when viewed
Press Dec Right
from the front is decreasing during discharge.
Press Increase The pressure is at least 10 % higher than that 5 minutes ago.
Press Decrease The pressure is at least 10 % lower than that 5 minutes ago.
The messages "Press Increase" and "Press Decrease" are also displayed in a
normal condition such as when the pumps are starting up or stopped.
If [Press Dec Right] is displayed, it may indicate one of the following:
• Air remains inside the right pump head.
• There is a leak in the right pump head's seal.
• The right pump head's inlet check valve or the left pump head's outlet check
valve are not operating properly.
If [Press Dec Left] is displayed, it may indicate that conditions described above
have occurred for the left pump head.
"Turning on/off Auto-Diagnostics《AUTO DIAGNOSTICS》" P.62
n Displaying Vacuum Pressure in Degassing Unit《DEGAS PRESSURE INT/EXT》
The screen displays the vacuum pressure of

the degassing unit built in or connected to
LC-40B X3 CL.
• [(OK)] is displayed when the vacuum
pressure is at a normal value.
• [(NG)] is displayed when the vacuum pressure is at an abnormal value.
LC-40B X3 CL 25
3 Operation
The [INT: DEGAS PRESSURE] window

displays [Disconnected] when the built-in
degassing unit is not used. The [EXT:
DEGASPRESSURE] window displays
[Disconnected] when the optional
degassing unit is not connected.
n Displaying the status of connection with PC/WS《PC/WS》
The screen displays the status of

connection with a PC/WS.
[Connected] is displayed when the
instrument is connected with a PC/WS.
This screen is displayed only when the
CBM-40 CL is connected.
[Disconnected] is displayed when the instrument is not connected with a PC/WS.
26 LC-40B X3 CL
3.5 Auxiliary Functions Screen (FUNCTION)

The auxiliary functions screen has six setting groups. Switch the groups with [↑] or [↓]
and press [→] to enter the screen of the desired setting group. Press [CE] to return to
the initial screen.
Setting Group
Description
Name
PARAMETER The group allows the user to set analysis parameters.
CONTROL The group enables specific operation.
The group allows the user to set parameters related to the system such
SYSTEM
as parameters of connection with external equipment.
The group allows the user to set parameters not related to the analysis
UTILITY
result such as display and buzzer parameters. 3
EDIT Time programs can be edited.
FILE Not used in CL model.
3.5.1 Auxiliary Functions (FUNCTION) List
n [PARAMETER] settings group
Name Description Remark Ref.

Initial value: 10
Sets the maximum pressure limit
P.MAX A/B Set value: P.30
for solvent delivery.
1.0 to 130.0 MPa
Initial value: 0
Sets the minimum pressure limit for
P.MIN A/B Set value: P.30
solvent delivery.
0 to 130.0 MPa
Makes fine adjustment of
Initial value: 0.45
COMP A/B compressibility correction of P.31
Set value: 0.00 to 3.00
solvent.
EXT.SV PORT
Not used in CL model* Initial value: 1 -
CH1/CH2/CH3
EVENT1 Not used in CL model* Initial value: 0 -
EVENT2 Not used in CL model* Initial value: 0 -
Initial value: 3
PURGE TIME Sets a purge execution time. P.33
Set value: 1 to 20 minutes
Initial value: 4.0
PURGE FLOW Sets a purge flow rate. Set value: P.33
0.1 to 10.0 mL/min
Sets maximum pressure limit at Initial value: 10.0
PURGE P.MAX P.33
purging. Set value: 1.0 to 10.0 MPa
Sets the delivery amount of the
RINSING Initial value: 1
optional rinsing pump when the P.33
OPERATE Set value: 0 to 5
pump is used.
LC-40B X3 CL 27
3 Operation

Initial value: 0
FLOW SLOPE Sets the time to reach the set flow
Set value: P.34
A/B rate during delivery.
0 to 30.00 minutes
* Do not change the factory default value.
n [CONTROL] settings group

Moves the plunger position. Initial value: 0
PLUNGER SET
Replace the plunger or the plunger Set value: P.34
A/B
seal. 0: Stop 1: L 2: R 3: M
Performs zero adjustment of
pressure screen.
ZERO ADJUST - P.35
* Pumps A and B are zero-adjusted
at the same time.
n [SYSTEM] setting group

Initial value: 0
Selects independent operation or
LOCAL MODE Set value: P.35
control via system controller.
0: Remote 1: Local
Sets address of the instrument for Initial value: A: 4, B: 5
LINK ADDRESS P.35
control via system controller. Set value: 1 to 11
CONTROLLER Selects the type of the connected Initial value: 1
P.36
LINK system controller. Set value: 0: Int 1: Ext
SELECT EVENT1 Not used in CL model.* Initial value: 0 -
SELECT EVENT2 Not used in CL model.* Initial value: 0 -
Initial value: 2
Set value:
PUMP CONFIG Input values according to the
1: Isocratic mode P.37
(SYS) system used.
2: High-pressure gradient
mode
Reduces the flow rate without
SYSTEM Initial value: 0
stopping the pumping when the P.38
PROTECT Set value: 0: Clear 1: Set
pressure exceeds the P.MAX value.
Initial value: 0
EXT.SV TYPE Not used in CL model.* Set value: -
0: - 1: 11AL 2: 11ALS
EXT.SV SERIAL Not used in CL model. Initial value: - -
EXT.SV CHANNEL Not used in CL model.* Initial value: 0 -
Initial value: 0
Sets the unit of the displayed Set value:
PRESSURE UNIT P.39
pressure. 0: MPa 1: kgf/cm2
2: bar 3: psi
* Do not change the factory default value.
28 LC-40B X3 CL
n [UTILITY] setting group

KEY CLOSE Disables the key input. - P.39
Sets the brightness of display Initial value: 4
BRIGHTNESS P.39
screen. Set value: 1 to 4
Initial value: 0
BEEP MODE Sets the operation of buzzer. Set value: P.40
0: ON 1: Alarm 2: OFF
Initial value: 2
VOLUME Sets the buzzer volume. P.40
Set value: 1 to 3
The operation panel turns off after Initial value: 1
DISP OFF TIME P.40
the time set here elapses. Set value: 0 to 10 minutes
Specifies whether to display the Initial value: 0
DIRECT KEY
confirmation screen when the Set value: P.40
3
MODE
direct key is operated. 0: Confirm 1: Direct
n [EDIT] group
Use this group to create a time program. For details, see "3.7 Creating Time Program"
P.63.
n [FILE] operation group
This function is not used because events are not used in the CL model.
Name Description Remark

Initial value: 0
FILE NUMBER Selects a program file No.
Set value: 0 to 9
Initial value: 0
FILE COPY Copies a file.
Set value: 0 to 9
FILE DELETE Deletes a time program from a file. -
About files:
The instrument can store up to 10 files of flow rate and other parameter values, and
time programs in memory. File No. 0 to 9 are assigned to each file. The auxiliary
functions except [EXT.SV PORT] are common functions.
Use [FILE NUMBER] of the file operation group to switch the files.
3.5.2 [PARAMETER] Setting Group
This setting group is relevant to solvent delivery parameters.
LC-40B X3 CL 29
3 Operation
n Setting the maximum pressure limit during pumping《P.MAX A/B》
When the delivery pressure exceeds the set

value, an error will occur and stop delivery.
Input the value with the numeric keypad
and press [Enter].
Setting Range Setting Step

1.0 to 130.0 MPa 0.1 MPa
10 to 1326 kgf/cm2 1 kgf/cm2
10 to 1300 bar 1 bar
142 to 19000 psi 1 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
n Setting the minimum pressure during delivery《P.MIN A/B》
When the delivery pressure falls below the

set value for a specific time, an error will
occur and stop delivery.
Input the value with the numeric keypad
and press [Enter].

0 to 130.0 MPa 0.1 MPa
0 to 1300 bar 1 bar
0 to 19000 psi 1 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
30 LC-40B X3 CL
n Setting the compressibility of solvent《COMP A/B》
Although the instrument corrects the

compressibility to suppress pulse increase
caused by the effect of the compressibility
of the solvent, setting a compressibility
suitable for the solvent used enables more
accurate correction. Mobile Phase compressibility (GPa)-1
Input the compressibility (GPa)-1 with the Water 0.45
numeric keypad and press [Enter]. The
Acetonitrile 1.20
initial value is 0.45.
Methanol 1.25
Ethanol 1.20
2-propanol 1.20
Hexane 1.60 3
Heptane 1.25
Cyclohexane 1.25
Ethyl Acetate 1.10
Chloroform 1.10
Benzene 1.00
Single solvent
Set the compressibility according to the table.
Mixture of water and organic solvent

If the concentration of the organic solvent is 50 % or less, "0.45" is recommended for
the compressibility of water. If the concentration is more than 50 %, make the value
closer to the compressibility of organic solvent according to the mixture ratio.
Example) When the ratio between water and acetonitrile is 50 : 50, set "0.45".
When the ratio between water and acetonitrile is 30 : 70, set "0.75".
In other cases or when rigorous adjustment is required
1 Connect a resistance tube (or column) that can create pressure similar to the
desired delivery pressure at 1.0 mL/min or less and wait until delivery becomes
stable.
2 While monitoring fluctuation on the pressure monitor of the instrument,

gradually increase the set value of [COMP] from 0.45 in 0.05 increments to find
the COMP value with the minimum fluctuation on the pressure monitor.
LC-40B X3 CL 31
3 Operation
n Setting the flow path of the mobile phase switching valve connected to the back of
the instrument《EXT.SV PORT CH1 / CH2 / CH3》

Do not change the factory default value.
n Setting the EVENT1 output terminal《EVENT1》

Set "ON" (close) / "OFF" (open) of the

[EVENT1] output (relay contact) on the
back of the instrument.
Set
EVENT1 Output
Value
0 Relay 1 OFF
1 Relay 1 ON
n Setting the EVENT2 output terminal《EVENT2》

Set "ON" (close) / "OFF" (open) of the

[EVENT2] output (relay contact) on the
back of the instrument.
Set
EVENT2 Output
Value
0 Relay 2 OFF
1 Relay 2 ON
32 LC-40B X3 CL
n Setting the purging time《PURGE TIME》
Set the purging time of pumps A and B to

the same time period.
The value can be set in the range from 1
minute to 20 minutes in one minute
increments.
n Setting the purging flow rate《PURGE FLOW》
Set the purging flow rate of pumps A and

B to the same flow rate. This setting is
effective for both purging with the
[PURGE] operation in the instrument and
auto purging with the system controller. 3
The value can be set in the range from 0.1 mL/min to 10.0 mL/min in 0.1 mL/min
increments.
The flow rate set here is approximate for replacing the mobile phase in the
pump.
n Setting the maximum pressure during purging《PURGE P.MAX》
Set the maximum pressure limit for auto

purging to the same value.
For a purging operation started by the

[PURGE] key of the instrument, the
maximum pressure limit function works at
2.0 MPa and displays the error shown in
the figure as well as stopping delivery.

1.0 to 10.0 MPa 0.1 MPa
10 to 100 bar 1 bar
142 to 1451 psi 1 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
n Setting the delivery amount of the rinsing pump《RINSING OPERATE》
Set the delivery amount of the optional

rinsing pump. The rinsing pump is a
standard unit for LC-40B X3 CL.
During delivery or purging operation, if
the setting is "standard", the rinsing pump
LC-40B X3 CL 33
3 Operation
starts with 6-second delivery and then delivers rinsing solution for 2 seconds at intervals
of 1 minute. The other settings double or triple the 2-second delivery time.
The user can set the delivery amount to one of the four levels below.
Selecting "0" disables the rinsing pump.
Based on the delivery amount per hour in the following table, prepare necessary the
amount of the rinse solution for the analysis time.
• The delivery amount in the table is approximate for rinsing.

• Select "0" when not using the rinsing pump.
• The approximate delivery amount necessary for replacing the rinse solution in the rinsing
flow path is approximately 10 mL.
• To replace the rinse solution in the rinsing flow path, select "5" and operate the pump
for approximately 10 minutes for LC-40B X3 CL.
LC-40B X3 CL
Set Value Delivery Amount per Hour (mL/h) Remark

0 0 The rinsing pump is disabled.
1 1.5 Half of standard
2 3 Standard
3 6 Twofold amount of standard
4 12 Fourfold amount of standard
5 Purge (90) Operates continuously
n Setting the time to reach the set flow rate after the start of delivery《FLOW SLOPE
A/B》
Set the time to reach the set flow rate

after the start of delivery.
The value can be set in the range from
0 minutes to 30.00 minutes in 0.01 minute
increments.
Selecting "0" disables this function.
3.5.3 [CONTROL] Setting Group
n Setting the plunger stop position《PLUNGER SET A/B》
Set the plunger stop position. This setting

is used for maintenance.
[PUMP] on the display is highlighted and
the plunger starts moving. [PUMP] on the
display stops being highlighted after the
plunger finishes moving.
34 LC-40B X3 CL
Set Value Function

0 The plunger stops moving.
The plunger on the left when viewed from the front is retracted.
1
In this state, the left pump head is removed.
The plunger on the right when viewed from the front is retracted.
2
In this state, the right pump head is removed.
3 The plunger stops at the middle position.
n Zero adjustment of pressure sensor《ZERO ADJUST》
Set the zero point of the pressure sensor

for pumps A and B at the same time.
1 Open the drain valves of pumps A and B to remove pressure completely.
2 Press [Enter].
3.5.4 [SYSTEM] Setting Group
This group is relevant to the system configuration such as connection with external
equipment.
n Selecting the local/remote mode《LOCAL MODE》
When the system controller is connected,

the user can select whether to operate the
instrument independently or with the
system controller.
Set Value Mode Function

0 Remote The instrument is operated with the system controller.
1 Local The instrument is operated independently (local mode).
n Setting the link address《LINK ADDRESS》
Set the address of pump A (channel

number) for connecting this instrument to
a system controller.
Set the address of pump A only.

The address of pump B is automatically set to the value equal to the set pump A address
plus 1.
LC-40B X3 CL 35
3 Operation
For example, if the user sets LINK ADDRESS to "3", the pump A address is set to 3 and
the pump B address is set to 4.
Address Function
1 to 11 A channel number for connecting with the system controller
n Setting the link destination of the system controller《CONTROLLER LINK》
Select the type of the connected system

controller.
Set Value Function

0 Not used in CL model.
The instrument is connected to an external system controller such as CBM-40
1
CL via an optical cable connected to the [REMOTE] connector.
n Setting the function of the EVENT1 output terminal《SELECT EVENT1》

Set the mode of controlling external

equipment via the [EVENT1] output (relay
1).
Set Value Function

0 The relay contact is controlled with the [EVENT1] set value.
1 The relay contact 1 [EVENT1] is used as a start signal of a time program.
If the SELECT EVENT1 function is used, the corresponding EVENT parameter is

disabled.
36 LC-40B X3 CL
n Setting the function of the EVENT2 output terminal《SELECT EVENT2》

Set the mode of controlling external

equipment via the [EVENT2] output (relay
2).
Set Value Function

3
0 The relay contact is controlled with the [EVENT2] set value.
1 The relay contact 2 [EVENT2] is used as an error output signal.
If the SELECT EVENT2 function is used, the corresponding EVENT parameter is

disabled.
n Setting the system parameter《PUMP CONFIG (SYS)》
Input values according to the status of the

system used.
Set Value Function

To use pump A and pump B independently or control them through the
1
system controller (isocratic mode).
2 To operate the system in high pressure gradient mode.
The system functions normally only with the above values. Values other than the
above cannot be set.
Setting the value to "1" (isocratic mode)

causes the initial screen to be displayed as
shown in the figure.
The first row shows the flow rate and
pressure of pump A and the second row
those of pump B. The flow rates are
configurable.
LC-40B X3 CL 37
3 Operation
n Setting the system protection《SYSTEM PROTECT》
When [P.MAX] is active, the flow rate is

reduced in increments of a half rate while
delivery is continued until the pressure
falls below [P.MAX].
Set Value Function

0 Do not use system protection.
1 Use system protection.
To clear a [P.MAX] error, press [CE]. The alarm stops and the flow rate returns to that
before the error.
n Selecting the type of external solenoid valve《EXT.SV TYPE》

Select the type of flow switching valve

(optional) connected to the [SV] connector
on the back of the instrument.
Set Value Function
0 Do not use FCV.
n Inputting the serial number of the external flow path switching valve《EXT.SV SERIAL》
Input the serial number of the flow path

switching valve connected to the SV
connector on the back of the instrument.
38 LC-40B X3 CL
Selecting the flow path of the solenoid

valve unit《EXT.SV CHANNEL》
Set Value Port

0 The port does not pass through a flow path of FCV-11AL/FCV-11ALS
n Setting the unit of the displayed pressure《PRESSURE UNIT》

3
Set the unit of the displayed pressure.
Set Value Unit of Displayed Value

0 MPa
1 kgf/cm2
2 bar
3 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
3.5.5 [UTILITY] Setting Group
This setting group is relevant to the display monitor.
n Disables the key entry《KEY CLOSE》
Press [Enter] to disable key input.

From this point, keypad input is disabled.
To release this function, press [Enter] while pressing [CE].
n Setting the brightness of display screen《BRIGHTNESS》
Sets the brightness of the display screen.

The value range is 1 to 4, and 4 is the
brightest.
LC-40B X3 CL 39
3 Operation
n Setting the operation of buzzer《BEEP MODE》
Sets the buzzer sound.
Set Value Function

The key input sound, as well as the alarm sound to go off when an error
0
occurs, are activated. (Default setting)
1 Only the alarm sound that goes off when an error occurs is activated.
2 All the buzzer sounds are deactivated.
n Setting the operation of buzzer volume《VOLUME》
Set the buzzer sound level. A larger setting

value increases the sound level.
n Setting the time to turn off the operation panel automatically《DISP OFF TIME》
Sets the time elapsed before the operation

panel automatically turns to the sleep
mode, when no operations are performed
on the instrument.
The setting range is from 0 to 10 (min), in which the step can be set by minute. If 0
(min) is input, the operation panel remains on.
Setting "0" minutes causes the display screen to be always on. If the display
screen is always on, some dots frequently lighting will deteriorate in brightness
in a shorter time.
n Setting the direct keys《DIRECT KEY MODE》
Allows displaying the confirmation screen

when a direct key ([PUMP], [RUN], or
[PURGE]) is operated to prevent
unintended operation.
For example, setting this function to "0"

(Confirm) and then pressing [PUMP]
displays the confirmation screen shown in
the figure. Pressing [Enter] starts the
operation.
Pressing [PURGE] when starting purge does not display the confirmation screen. The screen,
however, is displayed when the purge is stopped.
40 LC-40B X3 CL
3.5.6 [FILE] Operation Group
This function is not used because events are not used in the CL model.
n Specifying the file number《FILE NUMBER》
The instrument allows the user to create

and store a maximum of ten time
programs. The program files can be 3
selected with the parameter.
Select the file number from 0 to 9.
Input the file number with the numeric keypad and press [Enter].
n Specifying the file copy destination《FILE COPY》
The program content of the currently

selected file (indicated by the number of
[FILE NUMBER] above) is copied to the file
of a specified number.
Input a file number with the numeric
keypad and press [Enter].
n Deleting a file《FILE DELETE》
The time program of the currently selected

file is deleted.
Press [Enter].
LC-40B X3 CL 41
3 Operation
3.6 VP Functions Screen

The VP functions screen has four setting groups. Switch the groups with [↑] or [↓] and
press [→] to enter the screen of the desired setting group. Press [CE] to return to the
initial screen.
Setting Group Name Description

PRODUCT INFO The group is relevant to information of the instrument.
MAINTENANCE The group is relevant to maintenance of the instrument.
The group allows the user to check whether the instrument is
VALIDATION
operating correctly.
CALIBRATION The group is for calibration of the instrument.
3.6.1 List of VP Functions
n Product information group [PRODUCT INFO]
Name Function Ref.

SERIAL NUMBER Displays the serial number of the instrument. P.44
S/W ID Displays the S/W version number. P.44
n Maintenance information group [MAINTENANCE]
Name Function Ref.

TOTAL OP TIME Displays the total operation time of the instrument. P.44
Displays or reset the total delivery amount of the
L/R SEAL USED A currently used plunger seal and the delivery amount that
requires replacement (for the pump A).
P.44
Displays or reset the total delivery amount of the
L/R SEAL USED B currently used plunger seal and the delivery amount that
requires replacement (for the pump B).
Displays the remaining amount of mobile phase and set
MOBILE PHASE A/B P.45
an amount.
Specifies a threshold to cause an alarm of low mobile
ALARM LEVEL A/B P.45
phase level.
PART REPLACEMENT Input the part number of the replaced part when
P.45
A/B replacing the part.
MAINTENANCE LOG
Displays the maintenance log. P.45
A/B
OPERATION LOG A/B Displays the log of password change, parameter reset, etc. P.46
ERROR LOG A/B Displays the error log. P.46
Displays or reset the operation time of the degassing unit
DGU OP TIME INT／EXT P.47
built in or connected to the instrument.
42 LC-40B X3 CL
n Validation support information group [VALIDATION]
Name Function Ref.

DATE Displays or set the date. P.48
TIME Displays or set the time. P.48
MEMORY CHECK Checks ROM/RAM. P.48
LEAK SENSOR CHECK Checks operation of the leak sensor. P.48
PULSE CHECK A/B Checks the pulse. P.49
FLOW CHECK A/B Checks the flow rate. P.50
PRESS LIMIT CHECK Checks that the maximum and minimum pressure
P.54
A/B functions work correctly.
n Calibration support information group [CALIBRATION] 3

Name Function Ref.
Input PASSWORD*1 Input a password. P.55
OPERATION MODE Not used in CL model.*2 -
DATE FORMAT Changes the displayed order of year, month and day. P.55
INITIALIZE PARAM Initializes parameters. P.56
PARAMETER LOCK To prohibit change of parameters. P.56
PARTS MGMT TOOL Sets the usage of the parts management tool. P.56
PASSWORD SETTING Changes the password. P.56
Displays or set the parameters of the connected system
CBM PARAMETER P.58
controller.
LEAK THOLD Sets the operation level of the leak sensor. P.60
FLOW COMP FACTOR
Not used in CL model.*2 -
A/B
PRESS COMP FACTOR
Not used in CL model.*2 -
A/B
PLUNGER SEAL A/B Sets the time to replace the plunger seal. P.62
P.MAX OVERRIDE Sets override of the maximum pressure. P.62
AUTO DIAGNOSTICS Turns on/off Auto-Diagnostics. P.62
*1 In the calibration support group, an invalid password prevents the user from going further than
[OPERATION MODE] even if the user presses [Enter].
*2 Do not change the factory default value.
LC-40B X3 CL 43
3 Operation
3.6.2 Product Information Group (PRODUCT INFO)
The group is relevant to information of the instrument.
n Showing the serial number《SERIAL NUMBER》
Displays the serial number of the

instrument.
n Displaying the S/W version number《S/W ID》
The screen displays the S/W name (model

name) and version.
3.6.3 Maintenance Information Group (MAINTENANCE)
The group is relevant to maintenance information of the instrument.
n Showing the total operation time《TOTAL OP TIME》
Displays the total cumulative operating

time of the instrument.
n Displaying the delivery amount of the left/right plunger seal《L/R SEAL USED A》《L/R
SEAL USED B》
The screen displays the total delivery

amount of the currently used plunger seal
and the delivery amount that requires
replacement (unit: L).
If the parts management tool (PARTS
MGMT TOOL) is not used, press [del] after replacing the seal. The total delivery amount
is reset to "0" and the reset date is recorded in the maintenance log.
• The plunger seal on the left when viewed from the front is shown as "L".
• When PARTS MGMT TOOL is active, the delivery amount cannot be reset on the operation
panel. For the operation procedure, refer to the PARTS MGMT TOOL instruction manual.
44 LC-40B X3 CL
n Displaying and setting the remaining amount of mobile phase《MOBILE PHASE A/B》
The screen displays the remaining amount

and set amount of the current mobile
phase.
When replacing the mobile phase, input
the set amount of the mobile phase in the
unit of mL and press [Enter].
The remaining amount is automatically
calculated when solvent is delivered.
n Setting a threshold to cause an alarm of low mobile phase level《ALARM LEVEL

A/B》
The warning screen is displayed when the

mobile phase level has fallen to the set
3
percentage. Note that delivery continues.
Input the set value with the numeric
keypad and press [Enter]. Setting the value
to 0 % disables the low mobile phase level
alarm.
n Inputting a replacement part number《PART REPLACEMENT A/B》
When the parts management tool (PARTS

MGMT TOOL) is not used, input the part
number when replacing a general part.
The input part number is stored in the
maintenance log.
When PARTS MGMT TOOL is active, a

part number cannot be input on the
operation panel. Input the number
from PARTS MGMT TOOL.
"Displaying the maintenance log《MAINTENANCE LOG A/B》" P.45
When PARTS MGMT TOOL is active, the delivery amount cannot be reset on the operation
panel. For the operation procedure, refer to the PARTS MGMT TOOL instruction manual.
n Displaying the maintenance log《MAINTENANCE LOG A/B》
This displays twenty records of previously

replaced parts with the dates of their
replacement. After press [→], press [↓]
several times to display the replaced parts
in order.
The figure shows an example that
"part number 228-48249-96 was replaced
on April 1, 2019".
LC-40B X3 CL 45
3 Operation
The table lists the contents displayed in the second row of a maintenance log.
Message Description Ref.

L SEAL REPLACED The left plunger seal was replaced. P.44
R SEAL REPLACED The right plunger seal was replaced. P.44
P/N: XXX-XXXXX-XX The displayed part number was replaced. P.45
The setting value (******) was entered in DGU OP
DGU OP TIME: ****** P.47
TIME.
n Displaying the operation log《OPERATION LOG A/B》
This displays ten records of previous

modifications such as password change and
parameter reset with the dates of the
modifications. After press [→], press [↓]
several times to display the operation log
records in order.
"the password was changed on April 1,
2019".
The table shows the messages displayed in the second row of a maintenance log.
Message Description Ref.

INITIALIZE PARAM The parameters were initialized. P.56
PARAM LOCK ON*1 The parameter lock function was turned ON.
PARAM LOCK OFF*1 The parameter lock function was turned OFF.
P.56
When the parameter lock function was ON, a user
PARAM LOGIN*1 entered the password and moved on to the next
screen.
CHANGE PASSWORD*1 The password was changed. P.56
USE PARTS MGMT The PARTS MGMT TOOL was set to "Use".
P.56
NOT USE PARTS MGMT The PARTS MGMT TOOL was set to "NotUse".
*1 Only OPERATION LOG A displays logs.
n Displaying the error log《ERROR LOG A/B》
This displays ten records of previous errors

with the dates of the errors.
After press [→], press [↓] several times to
display the error log records in order.
"a maximum pressure error occurred on
April 1, 2019".
For the messages displayed in [ERROR
LOG], refer to the System Guide.
System Guide "5.4 Error Log List"
46 LC-40B X3 CL
n Displaying the operation time of the degassing unit《DGU OP TIME INT/EXT》
The screen displays the operation time of

the degassing unit built in or connected to
the instrument.
When the operation time is input, count
of the operation time of the degassing
unit starts from the input time. The input set value is stored in the maintenance log.
If [999999 h] is set, the display shows "******" and the operation time will not be
counted.
• To activate this function for an external degassing unit, supply power from
the instrument to the degassing unit and make sure that the pressure signal
cable is connected correctly. If more than one pump is used, be sure to connect
the power cable and pressure signal cable to the same pump. For how to 3
connect, refer to the System Guide.
• The shutdown function of CBM-40 CL or LC-Workstation can stop the degassing
unit. For details, refer to the instruction manual of the system controller or
LC-Workstation.
• Approximate time to replace the vacuum pump of the degassing unit is 3 years
or 8000 hours of operation time. Contact your Shimadzu representative if you
need to replace the vacuum pump.
LC-40B X3 CL 47
3 Operation
3.6.4 Validation Support Information Group (VALIDATION)
The group allows the user to check whether the equipment is operating correctly.
n Entering date《DATE》
This allows the user to view or input the

date in this screen. The value is reset to
the initial value "00-00-00" when the
power switch on the back is turned off.
The value is transmitted when the system
controller is connected for control.
To delete the input value, press [del].
The figure is an example of April 1, 2019.
n Entering time《TIME》
This allows the user to view or input the

time in this screen. The value returns to
the initial value "00:00:00" when the
power is turned off. The value is
transmitted when the system controller is
connected for control.
To delete the input value, press [del].
The figure is an example of 3:01:01 p.m.
n Checking the memory《MEMORY CHECK》
Runs the memory check on ROM and RAM.

Pressing [Enter] starts the memory check.
After the check, the result is displayed.
n Checking the leak sensor《LEAK SENSOR CHECK》
Performs the operation test for the leak sensor.
1 Use a syringe filled with water to wet

the sensor unit at the bottom of the
leak sensor.
48 LC-40B X3 CL
2 Wait about 10 seconds and then press

[Enter].
If the sensor detects a leakage, [Good] will
be shown.
If not, [No Good] will be shown.
3 Press [CE].
The result display will be cleared.
If the result is [No Good], adjust the detection level with [LEAK THOLD] function in the
Calibration Support Group. 3
"Setting the operation level of leak sensor《LEAK THOLD》" P.60
After wetting and testing the leak sensor, wipe away the water on the tray
completely. When wiping away the water, do not apply pressure to the leak
sensor, the sensitivity decreases if the sensor unit touches the wall.
"4.12 Cleaning of the Leak Tray" P.108
n Checking the pulse《PULSE CHECK A/B》
When checking operation, connect a resistance tube to the pump outlet to create 5 MPa
to 10 MPa pressure at 1 mL/min during delivery.
1 Press [Enter].
LC-40B X3 CL 49
3 Operation
2 Set the acceptance criterion (MPa) of

pressure variation (pulse).
Input the set value with the numeric
keypad and press [Enter]. Start delivery at
1 mL/min.
The value range is 0.05 to 1.00 MPa.
• When setting 0.20 MPa as the acceptance criterion, input a value as shown in the figure.
• Standard of the acceptance criterion (MPa) : 0.20 MPa or less (When connecting the
resistance tube 4 m x 0.1 mm I.D and delivery of water at 1 mL/min.)
The measurement starts one minute after

delivery is started. During measurement,
the set flow rate (1 mL/min), measured
pressure, pressure variation, and remaining
time are displayed.
After measurement, the panel displays the

measured pressure variation with pass or
fail, and then the results and the
measurement date and time are stored in
the memory of the instrument.
Before measurement, make sure that the compressibility correction parameter of

the solvent is correctly set.
n Checking the flow rate《FLOW CHECK A/B》
to 10 MPa pressure during delivery.
According to your measurement method, measure the flow rate in the procedure
described below.
Set Value Measurement Method

The flow rate is measured based on the time that was required to deliver
0
the specified volume. (volumetric method)
The flow rate is measured based on the weight of water delivered in the
1
specified time. (gravimetric method)
2 A flow meter is used for measurement
Before measurement, make sure that the compressibility correction parameter of

the solvent is correctly set.
50 LC-40B X3 CL
To use the volumetric method
1 Set the flow rate and start delivery.
2 Confirm that the delivery stabilized and then measure the time required for the
solvent accumulated in a measuring flask to reach 5 mL.
3 In the [FLOW CHECK A] screen, press

[Enter].
3
4 In the screen to select a measurement
method, input "0".
5 Input the measured flow rate.

The value can be set between 0.1 mL/min
and 3.0 mL/min.
6 Set the acceptance criterion of the

flow rate accuracy in "ML".
The value can be set between 0.01 mL and
1.00 mL.
For example, if the set flow rate is 1 mL/min and the criterion is ±2 %, input 0.02 mL.
7 Input the measurement time (seconds).
The flow rate accuracy (%) and pass/fail

result are displayed.
To use the gravimetric method
1 Measure the weight (g) before delivery.
LC-40B X3 CL 51
3 Operation
3 Confirm that the delivery stabilized and then wait a certain time for water to
accumulate in the measuring flask.
4 Measure the weight (g) before delivery.

[Enter].
6 In the measurement method selection

screen, input "1".

and 3.0 mL/min.

flow rate accuracy in mL.
The value can be set between 0.01 mL and
1.00 mL.
9 Input the weight (g) measured before

delivery.
10 Input the measurement time (seconds).
52 LC-40B X3 CL
11 Input the weight (g) measured after

delivery.

To use a flow meter
3
2 After confirming the delivery stabilizes, measure the flow rate.

[Enter].
4 In the measurement method selection

screen, input "2".

and 3.0 mL/min.

flow rate accuracy in mL.
The value can be set between 0.001 mL
and 1.000 mL.
LC-40B X3 CL 53
3 Operation
7 Input the flow rate measured with a

flow meter.

n Checking operation of the maximum and minimum pressure functions 《PRESS LIMIT
CHECK A/B》
to 10 MPa pressure at 1 mL/min during delivery.
1 Press [Enter].
Start delivery at 1 mL/min. The pressure is

monitored for 1 minute and the maximum
pressure (P.MAX) and minimum pressure
(P.MIN) are automatically set.
Then the flow rates are automatically set

to 1.5 mL/min and 0.5 mL/min, and
whether the P.MAX and P.MIN functions
work correctly is checked.
The result is displayed after checking is

completed.
54 LC-40B X3 CL
3.6.5 Calibration Support Information Group (CALIBRATION)
The group is for calibration of the instrument.
The instrument is tuned before shipment. Do not modify values unnecessarily.
n Inputting the password《Input PASSWORD》
The password must be input by the system

administrator.
Input the five-digit numbers by using the
numeric keypad, and press [Enter].
3
Be sure to input the five numbers. The default password is [00000].
When the password matches, the [OPERATION MODE] screen below appears on the screen.
If the password does not match, the user cannot go further.
n Setting the operation mode《OPERATION MODE》
This function is not used in the CL model.

The factory default value : 0
n Changing the displayed order of year, month and day《DATE FORMAT》
Change the displayed order of year, month

and day.
Input the value by using the numeric
keypad, and press [Enter].
Set value Display

0 Year, month, and day (YMD)
1 Day, month, and year (DMY)
2 Month, day, and year (MDY)
LC-40B X3 CL 55
3 Operation
n Initializing the parameters《INITIALIZE PARAM》
Initialize the parameters and the time

programs.
Press [Enter] to return to the default
values of the parameters and to delete the
time programs.
After the parameter initialization
completes, the instrument is automatically
restarted.
n Setting the parameter lock《PARAMETER LOCK》
This prevents users other than the

administrator from changing parameters
except the flow rate by prohibiting screen
transition to the auxiliary functions screen
and VP functions screen.
If [1] (On) is set, a password input screen is displayed when the user enters the setting
screen. If the user modifies the setting or inputs the password to enter the setting screen,
the operation is recorded in the operation log.
"Displaying the operation log《OPERATION LOG A/B》" P.46
n Setting the usage of the parts management tool《PARTS MGMT TOOL》
Sets the usage of the parts management

tool.
For details on the parts management tool
(PARTS MGMT TOOL), refer to the PARTS
MGMT TOOL instruction manual.
Set value Description

0 Do not use PARTS MGMT TOOL.
1 Use PARTS MGMT TOOL.
When the setting is changed, the change is recorded in the operation log.
"Displaying the operation log《OPERATION LOG A/B》" P.46
n Changing the password《PASSWORD SETTING》
Enables to change the password.
1 Press [Enter].
The input screen is displayed.
56 LC-40B X3 CL
2 Input the five-digit number by using

the numeric keypad, and press [Enter].
3 To confirm, input the same password

that was input in step 2 again.
When the input is completed and the
password is changed, the message
"PASSWORD CHANGED" is displayed. Then,
the password change is recorded in the
operation log.
If the password input is not correct, the
message "PASSWORD WRONG" is
displayed. At this stage, the password has 3
not been changed yet.
4 Press [Enter] to return to the title screen.
LC-40B X3 CL 57
3 Operation
n Showing/setting CBM parameter《CBM PARAMETER》
This allows the user to view or set the

parameters of CBM-40 CL that controls the
instrument.
Press [→] to enter the CBM parameter setting screen.

Press [↓] or [↑] several times to select the desired item.
Pressing [←] at an item in the screen returns the display to the screen as shown in the
figure.
If CBM-40 CL is not connected (it is not set the link address), pressing [→] does not bring
the user to the CBM parameter setting screen.
"Setting the link address《LINK ADDRESS》" P.35

"Setting the link destination of the system controller《CONTROLLER LINK》" P.36
List of CBM Parameters
Display Description
SERIAL NUMBER Displays the serial No. of CBM.
S/W ID Displays the program version No. of CBM.
INTERFACE Sets the transmitting protocol to data processing unit.
ETHERNET SPEED Sets the transmitting speed of Ethernet.*1
USE GATEWAY Sets usage of default gateway or DHCP server.*1
IP ADDRESS Sets IP address of CBM.*1
SUBNET MASK Sets subnet mask.*1
DEFAULT GATEWAY Sets default gateway.*1, *2
Select the communication distribution when connecting to an

TRS MODE
LC workstation or a Chromatopac.
*1 Available only to show, when not allowed to change on CBM-40 CL.

*2 Not available when [USE GATEWAY] is set to "Do not use Default Gateway".
Each parameter is activated after the system controller is restarted.
Refer to CBM-40 CL instruction manual for details of each parameter.
Showing the serial number《SERIAL NUMBER》

Displays the serial number of the system
controller which controls the instrument.
Showing the S/W version number《S/W ID》

Displays the S/W name (the same name as
the model name) and version of CBM
which controls the instrument.
58 LC-40B X3 CL
Setting the transmitting protocol to data processing unit《INTERFACE》

Sets the transmitting protocol between the
data processing unit and the system
controller which controls the instrument.
Set Value Transmitting Protocol

0 To connect with optical cable.
1 To connect with serial transmission. (RS-232C)
2 To connect with Ethernet.
Setting the transmitting speed of Ethernet《ETHERNET SPEED》

Sets transmitting speed of the system
controller Ethernet which controls the 3
instrument.
Set Value Transmitting Speed

0 Auto Detect
1 10 Mbps, Half Duplex
2 10 Mbps, Full Duplex
3 100 Mbps, Half Duplex
4 100 Mbps, Full Duplex
Setting the usage of default gateway or DHCP server《USE GATEWAY》

Sets usage of default gateway or DHCP
server of the system controller which
controls the instrument.
Set Value Description

0 Do not use Default Gateway.
1 Use Default Gateway.
2 Automatic acquisition of IP address from DHCP server.
Setting IP address《IP ADDRESS》

Sets IP address of the controller which
LC-40B X3 CL 59
3 Operation
Setting the subnet mask《SUBNET MASK》

Sets subnet mask of the controller which
Setting the default gateway《DEFAULT GATEWAY》

Sets the default gateway of the controller
which controls the instrument.
Setting the transmission mode《TRS MODE》

Select the communication distribution
when the controller is connected to an LC
workstation.
Set Value Description

0 Connects in manual setting at the system controller.
2 Can not be used.
3 Connects to Labsolutions.
Do not set values other than the set value above. Otherwise the instrument will
not work properly.
n Setting the operation level of leak sensor《LEAK THOLD》
Enables to set the threshold value for the

leak sensor.
When an error occurs in the leak sensor
check, set the value again using the
procedure below.
Calibration of the leak sensor must be performed by the system administrator.

The leak sensor detects liquid drops when the mobile phase leaks from the
plunger seal or tubes. When the sensor output value exceeds the predetermined
"threshold," the sensor issues an error and stops pumping.
1 Dip the sensor unit at the bottom of the leak sensor into water.
Upon detection of water, the leak sensor value (Actual Level) in the screen increases.
2 Read the maximum value of the leak sensor value (Actual Level).
Example) The maximum value here is "160".
60 LC-40B X3 CL
3 Wipe off the water near the leak sensor.

The leak sensor value (Actual Value) will decrease.
When wiping away the water, do not apply pressure to the sensor unit of the
leak sensor. The sensitivity decreases if the sensor unit touches the instrument
wall surface.
4 Calculate the threshold of the leak sensor based on the value read in step 2.
Formula to calculate the leak sensor threshold:
Threshold = (Max. value-100)×0.7+100
Example) The value obtained here is "142".
3
5 Press [Enter], input the value obtained
in step 4, and press [Enter].
The new "threshold" is stored.
n Setting the flow rate correction parameter (ALPHA)《FLOW COMP FACTOR A/B》

This allows the service personnel to set the

flow rate correction parameter.
n Setting the pressure sensor sensitivity correction factor《PRESS COMP FACTOR A/B》
Nou used in CL model.

This allows service personnel to set the

pressure sensor sensitivity correction factor.
LC-40B X3 CL 61
3 Operation
n Setting the time to replace the plunger seal《PLUNGER SEAL A/B》
This allows the user to set the time to

replace the plunger seal (unit: L).
"Displaying the delivery amount of the left/right plunger seal《L/R SEAL USED A》《L/R SEAL
USED B》" P.44
n Selecting how to set the maximum pressure《P.MAX OVERRIDE》

Set Value Setting Method

0 To set the value with the system controller.
1 To set the value on the pump.
n Turning on/off Auto-Diagnostics《AUTO DIAGNOSTICS》
This allows the user to turn on/off

Auto-Diagnostics.
Set Value Setting Method

0 Auto-Diagnostics is off.
1 Auto-Diagnostics is on.
"Displays of Condition《CONDITION A/B》" P.24
62 LC-40B X3 CL
3.7 Creating Time Program
• The function of the time program is not available when controlled by the
system controller. Configure from the workstation. See " system guide 2.2
Editing Methods"
• Refer to the following procedure when using this function in local mode.
The instrument can run a time program with a flow rate and other parameters. A created
time program is saved in a file. Check for file numbers in use before creating a time
program.
See "Specifying the file number《FILE NUMBER》" P.41 of the auxiliary function to view
or specify file numbers.
The time program function is available only when PUMP CONFIG (SYS) is set to "2" (high 3
pressure gradient mode).
3.7.1 Time Program Command List
The commands for the time program are listed below.
Com-
Description Setting Range Remark Ref.
mand
0 to Minimum unit:
FLOW Sets flow rate -
10.0000 mL/min 0.0001 mL/min
Specifies concentration of
Minimum unit: *1
BCONC mobile phase B 0 to 100 %
0.1 %
0 to 100 %
Opens or closes the
solenoid valve of 0, 1, 2, 3, 12, 13,
EXT.SV Not used in CL model. -
FCV-11AL/FCV-11ALS 23, 123
(optional)
Sets ON/OFF of the event
output (relay contact) on
EVENT 0, 1, 2, 12 Not used in CL model. -
the back of the
instrument
0 to 255
LOOP Repeats program * 0 means 256 - P.68
times.
STOP Stops program N/A - P.68

Switches to other
GOTO 0 to 9 Not used in CL model. -
programs (up to 10 files)
*1 The concentration of mobile phase A will be the value subtracted from the [BCONC] value from
100 (%).
Concentration A = 100 − [BCONC]
LC-40B X3 CL 63
3 Operation
3.7.2 Time Program Setting Screen
To create a time program, access the edit screen as described below.
1 Press [CE].
The initial screen is deisplayed.
2 Press [→] twice.

The auxiliary functions screen [FUNCTION]
is displayed.
3 Press [↓] several times until [EDIT] is displayed in the screen, move the cursor
to [EDIT], and press [→].
The time program editing screen is
displayed. The figure shows an example of
a time program that has ten steps already
created and 310 steps left available.
Display Description
USED The number of created steps in the selected file
LEFT The number of steps left available
4 Press [Enter] again to edit steps of the

time program.
Displays Description
TIME Time (minute) that elapsed from the start of the step
FUNC Command name
VALUE Set value
64 LC-40B X3 CL
3.7.3 Creating a High Pressure Gradient Program
Setting the concentrations of solvents B at individual time points in a time program can
change the concentrations of solvents A and B with time. When doing so, the following
is applicable to pump A (concentration of solvent A).
Concentration of solvent A (%) ＝ 100 (%) - Concentration of solvent B (BCONC) (%)
For the BCONC command, the parameter can be set in the range from 0 % to 100 %
in the minimum unit of 0.1 %.
The table shows an example of a time

program that changes the concentration of
solvent B as shown in the figure. (The
initial concentration of solvent B is 0.0 %.)
3
Step TIME (minute) FUNC VALUE (%)

1 20.00 BCONC 100.0
2 30.00 BCONC 100.0
3 30.01 BCONC 0.0
4 40.00 STOP -
1 Displays the time program setting

screen.
"3.7.2 Time Program Setting Screen"
P.64
2 Press [Enter].
The time (minute) setting screen is
displayed.
To set step 1
3 Press [2], [0], and [Enter] to set the

time.
LC-40B X3 CL 65
3 Operation
4 Press [↓] once, move the cursor to

[BCONC] (concentration of solvent B),
and press [Enter].
The [BCONC] command is selected, which
enables the user to input the solvent B
concentration.
5 Press [1], [0], [0], and [Enter].

This sets the concentration of solvent B to
100 % and finishes edit of step 1.
To set step 2
6 Press [3], [0], and [Enter] to set the

time.

and press [Enter].

concentration.
8 Press [1], [0], [0], and [Enter].
To set step 3
9 Press [3], [0], [.], [0], [1], and [Enter] to

set the time.

and press [Enter].
concentration.
11 Press [0] and [Enter].
66 LC-40B X3 CL
To set step 4
12 Press [4], [0], and [Enter].
13 Press [↓] several times until [STOP] is

displayed in the command selection
screen.
14 Press [Enter].
3
15 Press [CE] to save the time program.

Then the initial screen is displayed.
When inputting multiple steps, the user does not need to input the data in order of time
because they are automatically sorted.
3.7.4 Deleting a Step
To delete a step, display it and press [del].

See the following example of deleting the first step of the program created in "3.7.3
Creating a High Pressure Gradient Program" P.65.
1 In the same manner as creating the

program, display the desired step.
To display the second or subsequent

lines, press [↓] several times until the
desired step is displayed.
2 Press [del].
The first step of the program is deleted
and the second step is displayed.
LC-40B X3 CL 67
3 Operation
3.7.5 Starting and Stopping a Time Program
n Starting a Time Program
To start a time program after setting it,

perform the following.
Press [RUN] to start the program.
[RUN] on the display is highlighted.
n Stopping a Time Program
There are two ways to stop the program.

• Forcibly stopping a program being executed
Press [RUN] to stop the program.
[RUN] on the display stops being highlighted and the program stops.
• Stopping a program with the [STOP] command
"Setting a [STOP] command for a time program《STOP》" P.69
3.7.6 Commands Used for Time Programs Only
This section explains about the commands that can be set for time programs only.
n Setting a loop count for a time program《LOOP》
A [LOOP] command can repeat a program

a specified number of times.
Settings shown in the table repeat steps 1 and 2 three times at intervals of 30 minutes.
TIME VALUE
Step FUNC
(Minute) (mL/min)
1 15.00 FLOW 2.0000
2 20.00 FLOW 5.0000
3 30.00 LOOP 3
68 LC-40B X3 CL
3.8 Connection with the System Controller or Workstation
• After [LOOP] is repeated the specified number of times, the program

automatically stops.
• The VALUE of the [LOOP] command can be set to a maximum of 255. Note
that if it is set to "0", LOOP is executed 256 times.
n Setting a [STOP] command for a time program《STOP》
This allows the user to set the time to stop

a time program. Input the time in the last
step of a time program and press [↓]
several times until [STOP] is displayed.
Press [Enter] to set a [STOP] command.
In the following cases, do not set a [STOP] command at the end of the program. 3
1 When the time program is executed continuously
2 When the program files are linked through a [GOTO] command
3.8 Connection with the System Controller or Workstation
3.8.1 Setting the Instrument
To control the instrument from CBM-40 CL set the parameters as follows:
Setting
Set Value Remark
Parameter
"Selecting the local/remote mode《LOCAL MODE》"
LOCAL MODE 0: Remote
P.35
LINK ADDRESS Link address "Setting the link address《LINK ADDRESS》" P.35
CONTROLLER 0: Int "Setting the link destination of the system controller

LINK 1: Ext 《CONTROLLER LINK》" P.36
OPERATION Not used in CL model.

0: 40B X3 CL
MODE Do not change the factory default value.
3.8.2 Basic Parameters
A max. of 4 pump units can be connected to CBM-40 CL. Workstation can set flow rate
(pressure), maximum pressure limit, minimum pressure limit, pumping "ON"/"OFF", time
program, etc., and can control binary and ternary high-pressure gradient analysis
depending on the system configuration. Also, by combining the SIL-40 CL series
autosampler, the auto purging function is available. For details, refer to the system guide.
LC-40B X3 CL 69
3 Operation
3.8.3 Notes on Operation
• When connect LC-40B X3 CL to the system controller, be sure to connect both of A

and B pump. Otherwse the instrument may not work correctly.
3.9 Connection to External Input/Output Terminals
In the CL model, do not connect another external device with the black-white
event cable.
Wiring is done by service personnel during installation.
70 LC-40B X3 CL
4 Maintenance
4.1 Periodic Inspection and Maintenance
It is necessary to perform periodic inspections of this instrument to ensure its safe use.
It is possible to have these periodic inspections performed by Shimadzu service personnel
on a contractual basis.
For information regarding the maintenance inspection contract, contact your Shimadzu
representative.
This chapter takes pump A as an example to describe the maintenance and inspection
procedures of the instrument.
Turn off the main power switch on the back of the instrument and unplug
the power cable prior to maintenance when instructed.
Fire, electric shock or malfunction may occur.
For parts replacement, use parts and tools listed in "1.2 Component Parts" P.2
and "5.2 Maintenance Parts" P.115".
If any other parts or tools are used, part damage, injury, and malfunction may
occur.
Never remove the cover.

This may cause injury or a malfunction of the device.
Contact your Shimadzu representative if the cover must be removed.
LC-40B X3 CL 71
4 Maintenance
4.1.1 Prior to Inspection and Maintenance
• Loosen the male nuts (A and C) on the

column inlet and outlet with an 8-mm
wrench and then remove the column (B)
from the flow path. Attach the coupling
(D) to the male nut (A and C), and
tighten it with an 8 mm wrench.
• Replace the mobile phase in the flow lines with at least 20mL of water. When normal
phase solvent was used, replace the internal channel with 2-propanol and then with
water.
• Wipe away any dirt from the front panel and the main cover.
n Standard Reagents for Maintenance
Solvent Description Inspection/Maintenance Item

"4.3 Replacing the Plunger
Seal"
"4.4 Cleaning/ Replacing the
Plunger and Diaphragm"
"4.5 Rinsing the Check Valve"
"4.6 Performing an Ultrasonic
2-propanol sed for cleaning parts. Cleaning of the Check Valve"
"4.10 Cleaning/Replacing the
Suction Filter"
"4.11 Replacing the Drain
Valve"
"4.14 Cleaning of the
Automatic Rinsing Kit"
n Hardware Maintenance Supplies
Note that in addition to the following tools and parts are also required. The tools and
parts required for the maintenance of each part are listed at the beginning of each
section.
Item Description Inspection/Maintenance Item
Protective glasses glasses and gloves before
ALL Inspection/Maintenance
Gloves the maintenance.
72 LC-40B X3 CL
Item Description Inspection/Maintenance Item

Used for wiping and Seal"
Wiping paper
cleaning parts. "4.4 Cleaning/ Replacing the
Plunger and Diaphragm"
Beaker Used for cleaning parts.
Seal"
Seal"
"4.5 Rinsing the Check
Valve"
Ultrasonic bath Used for cleaning parts.
"4.10 Cleaning/Replacing the
Suction Filter"
"4.14 Cleaning of the
Automatic Rinsing Kit"
Used for gripping the
Tweezers plunger seal and backup
Seal"
ring.
4.1.2 List of Periodic Inspection and Maintenance 4
The maintenance and replacement periods listed in this table are presented only
as guidelines. These are not guarantee periods.
These will vary depending on usage conditions.
Inspection/Maintenance Replacement
Remark Ref.
Item Periods
• Sealing efficiency decreases when seals
are worn.
• As a guideline, seals should be
replaced after delivery of the
respective volumes listed below. (VP
fuction, [L(R)SEAL USED], shows the
total delivered volume.)
Replacement of Plunger
1 year P.82
Seal
Pumping Pressure Total Delivery
80 MPa (816 kgf/cm2) 30 L
Repeating high-pressure (above

100 MPa) and low-pressure (below
10 MPa) pumping could shorten the life
of seals.
Replace the plunger seal and the
Replacement of Plunger 1 year diaphragm when the plunger is
replaced. P.91
Replacement of
1 year -
Diaphragm
Replacement of Outlet
1 year - P.101
Check Valve
LC-40B X3 CL 73
4 Maintenance
Remark Ref.
Item Periods
Replacement of Inlet
1 year - P.101
Check Valve
Replace when particulates in mobile
Replacement of Line Filter 2 years P.102
phase clog the filter.
Replacement of Suction Replace when particulates in mobile
2 years P.104
Filter phase clog the filter.
Replacement of Drain Replace when there is a fluid leak from
3 years P.107
Valve the drain valve.
Replacement of Air Filter
1 year Contact your Shimadzu representative. -
(for FAN)
Pump ASSY lubrication 3 years Contact your Shimadzu representative. -
Replacement of Fuse 3 years Contact your Shimadzu representative. -
Cleaning of the
1 year - P.110
Automatic Rinsing Kit
Pumping mobile phase mixtures
containing acids such as trifluoroacetic
acid or formic acid can cause leakage
due to internal damage in the pump
Replacement of Pump head. In such cases, replace the pump
(2 years) -
Head head every two years, as a general
guideline.
(Pump heads do not need to be
replaced if only mobile phases without
acid are pumped.)
Inspection of Leak Sensor 1 year - P.48
n Optional Parts
Remark Ref.
Item Periods
Pumping mobile phase mixtures
containing acids such as trifluoroacetic
acid or formic acid can cause leakage
due to internal damage in the pump
Replacing Air Filter of head. In such cases, replace the pump
(1 years) -
Low Pressure Gradient Kit head every two years, as a general
guideline.
(Pump heads do not need to be
replaced if only mobile phases without
added acid are pumped.)
4.1.3 Check after Inspection and Maintenance
• After inspection and maintenance, check any leakage during pumping.
74 LC-40B X3 CL
• Return tubes after completing the work.
When the parts management tool （PARTS MGMT TOOL） is not used, input the
part number when replacing a part not registered in the PARTS MGMT TOOL.
"Inputting a replacement part number《PART REPLACEMENT A/B》" P.45
LC-40B X3 CL 75
4 Maintenance
4.2 Removing/Installing the Pump Heads

This section explains how to remove and re-attach the right pump head of pump A.
Removing and re-attaching the left-side pump head is also the same procedure.
Necessary tools
Part Name Quantity Part No.

Wrench (8 mm and 10 mm) 2 086-03002
Allen wrench (M5) 1 086-03805
Syringe 1 046-00038-01
Syringe needle 1 228-18216-91
n Prior to the maintenance perform the preparatory work.
"4.1.1 Prior to Inspection and Maintenance" P.72
n Removing the pump head
1 Perform either of the following to prevent the mobile phase solvent from
flowing out of the suction tube or pump head when removing the tubes around
the pump.
• Place the reservoir bottle at a position lower than the pump inlet.
• Empty the reservoir bottle and suction tube of the mobile phase solvent, loosen the
bushing of the suction filter, and remove it from the pump inlet.
2 Press [CE] to display the initial screen.
3 Press [→] twice.
4 Press [↓] once, move the cursor to [CONTROL], and press [→].
[PLUNGER SET] menu is displayed.
76 LC-40B X3 CL
5 Press [Enter].
The cursor blinks at the input position.
6 Press [2] and [Enter]. (To replace the

left plunger seal, press [1] and [Enter].)
[PUMP] on the display is highlighted and
after a while stops being highlighted. The
right plunger is now at the backmost
position.
In the conditions, remove the right pump head.
Before attaching the right pump head, perform steps 2 to 6 as well.
LC-40B X3 CL 77
4 Maintenance
7 Follow the instructions below to remove the tube (SUS pipe R) connecting the
check valve OUT and the pressure sensor.
1 While holding the check valve

OUT (A) with a 10-mm wrench,
loosen the check valve OUT side
male nut (B) of the SUS pipe R
with an 8-mm wrench.
2 Loosen the pressure sensor side
male nut (C) of the SUS pipe R
3 Hold the check valve OUT side

male nut ("a" in the figure) of
the SUS pipe R and then slightly
lift and pull out the SUS pipe R.
4 Pull the SUS pipe R to the right

to remove it from the pressure
sensor.
Though the orientation is

opposite to the right one
(SUS pipe R), remove the
left tube (SUS pipe L) in
the same procedure.
78 LC-40B X3 CL
8 Loosen the male nut PEEK (A) of the

inlet tube by hand and remove it from
the check valve IN (B).
9 Make sure that the drain tube of the

rinse flow line is inserted into the
drain bottle located below the pump,
and loosen the joint (A). Confirm that
the rinse solution inside the rinse flow 4
line is discharged, and tighten the joint
(A).
10 Gradually loosen the 2 hexagonal

socket screws using an Allen wrench
(M5) by alternating between the left
and right screw, and remove the pump
head.
Gently pull the pump head

horizontally along the plunger.
The plunger may be damaged
if the pump head is forcefully
removed.
LC-40B X3 CL 79
4 Maintenance
n Installing the pump head
1 Set the plunger position by following steps 2 to 6 in "Removing the pump head"
P.76.
2 Hold the pump head with the mark (a)

coming at the bottom right, tighten
the 2 hexagonal socket screws (B) with
an Allen wrench (M5) by tightening
the right and left screws alternately,
and attach the pump head (A).
Gently inset the pump head

horizontally along the plunger.
The plunger may be damaged
if the pump head is forcefully
inserted.
Tighten the left and right hexagonal socket screws 90 degrees at a time, and
then firmly tighten using the long side of the Allen wrench.
3 Tighten the male nut PEEK (A) of the

inlet tube to the inlet check valve (B)
by hand.
80 LC-40B X3 CL
4 Follow the instructions below to attach the tube (SUS pipe R) connecting the
check valve OUT and the pressure sensor.
1 Insert the end of the SUS pipe R

into the hole of the pressure
sensor.
2 Hold the check valve side end of

the SUS pipe R ("a" in the figure)
and then insert the male nut into
the check valve OUT (A). 4
3 While holding the check valve

OUT (A) with a 10-mm wrench,
tighten the SUS pipe R male nut
on the check valve OUT side with
an 8-mm wrench (B).
4 Tighten the pressure sensor side

male nut (C) of the SUS pipe R
Though the orientation is

opposite to the right one
(SUS pipe R), attach the
left tube (SUS pipe L) in
the same procedure.
LC-40B X3 CL 81
4 Maintenance
5 Set the delivery amount of the rinsing pump to [5].

"Setting the delivery amount of the
rinsing pump《RINSING OPERATE》"
P.33
6 Set the total flow rate to [0], set the concentration for pump B to [0], press the
[PUMP] key, and then press the [ENTER] key.
The rinsing pump operates. Wait approximately 10 minutes for the rinse fluid to exit
from the outlet of the rinse flow line.
"3.1.2 Setting Flow Rate" P.17
"3.2.2 Operation in Constant Flow
Delivery Mode" P.21
4.3 Replacing the Plunger Seal

A plunger seal and backup ring are attached inside the left and right pump heads. The
following section will explain the method for replacing the plunger seal on the right
side (when viewed from the front).
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Allen wrench (M5) 1 086-03805
Seal Installer/Remover 1 228-62458-41
Necessary parts
Part Name Part Type Part No.

Plunger Seal (UHP)
Consumable part 228-52711-93
(supplied with backup ring)
82 LC-40B X3 CL
n Prior to the maintenancePerform the preparatory work.
4.3.1 Replacing the Plunger Seal and Backup Ring
1 Remove the pump head, SUS pipe, and inlet tube according to the procedure
described in "Removing the pump head" P.76.
LC-40B X3 CL 83
4 Maintenance
2 Perform the following steps to replace the plunger seal and backup ring.
The plunger seal and the backup ring are installed inside the pump head. Use the
provided seal installer/remover to remove them.
Don't drop the seal installer/remover when using it.

Seal installer/remover may be damaged.
1 Wipe off the threaded tip on the

metal part (a) of the seal
installer/remover (A) with a
wiping paper soaked in
2-propanol.
2 With the metal part (a) of the

seal installer/remover (A) facing
downward, insert the seal
installer/remover (A) vertically
into the backup ring (B).
3 Turn the seal installer/remover (A)

180 degrees to 360 degrees
clockwise.
84 LC-40B X3 CL
4 Pull out the seal installer/remover

(A) upward.
The backup ring (B) will come off
from the plunger seal hole (b) of
the pump head (C).
If the backup ring is

still stuck, return to
step 3 and turn the
seal installer/remover
180 degrees more,
then retry this step.
5 Grip the backup ring (B) with two

fingers and turn the seal
installer/remover
counterclockwise, then remove
the backup ring from the seal
installer/remover.
metal part of the seal 4
installer/remover with a wiping paper soaked in 2-propanol.
7 With the metal part of the seal

installer/remover facing
downward, place the seal
installer/remover vertically above
the plunger seal and push it
slightly into the seal.
8 Turn the seal installer/remover (A)

clockwise until it comes into
contact with the pump head (C).
LC-40B X3 CL 85
4 Maintenance
9 Pull out the seal installer/remover

(A) upward.
The plunger seal (D) will come off
from the plunger seal hole (b) of
the pump head (C).
10 Place the pump head into a clean beaker containing 2-propanol, and clean it for
10 minutes using an ultrasonic bath.
11 Use a piece of wiping paper

soaked in 2-propanol to wipe the
plunger attachment portion (b)
inside the pump head (C).
• If seal material adheres to the plunger attachment portion of the

pump head, the airtightness cannot be maintained.
• If any scratches are found on the plunger attachment portion of
the pump head, replace the pump head.
12 Grip the plunger seal (D) with

two fingers and turn the seal
installer/remover
counterclockwise, then remove
the plunger seal from the seal
installer/remover.
metal part of the seal
installer/remover with a wiping paper soaked in 2-propanol.
14 Remove the cap (c) from the seal

installer/remover (A).
15 Wipe off the white protrusion (d)
and the surrounding area of the
seal installer/remover (A) with a
wiping paper soaked in
2-propanol.
86 LC-40B X3 CL
16 Put some drops of 2-propanol in

the plunger seal hole (b) of the
pump head (C) while paying
attention to spillage of
2-propanol.
Before putting in 2-propanol, screw the check

valves (E) into the pump head (C) so that
2-propanol does not leak from the holes for the
check valve.
17 Soak a new plunger seal and a new backup ring in 2-propanol in a clean beaker.
When gripping the plunger seal with

tweezers, do not touch the internal
hole surface. Also, to prevent damage,
do not grip the plunger seal and the
backup ring tightly with tweezers.
18 Take the new plunger seal (D) out

of the beaker and put it on the
white protrusion (d) of the seal
installer/remover (A).
Be sure that the plunger seal is moistened with 2-propanol when putting it
on the seal installer/remover.
LC-40B X3 CL 87
4 Maintenance
19 Insert the seal installer/remover

(A) vertically all the way into the
plunger seal hole (b) of the pump
head (B) and slowly pull out the
seal installer/remover upward.
The plunger seal will be installed
in the pump head.
20 Take the new backup ring (B) out

of the beaker and, with the
smaller diameter side facing the
seal installer/remover (A), put the
backup ring on the white
protrusion (d).
Be sure to attach the backup ring with

the correct orientation.
When the backup ring (B) is attached
correctly to the pump head, the larger
diameter side comes into contact with
the plunger seal (D).
Be sure that the backup ring is moistened with 2-propanol when putting it
on the seal installer/remover.
21 Insert the seal installer/remover

vertically all the way into the
plunger seal and slowly pull out
the seal installer/remover upward.
The new backup ring (B) will be
installed into the pump head (C).
88 LC-40B X3 CL
22 Put the cap (c) on the white

protrusion (d) of the seal
installer/remover.
3 Attach the pump head, SUS pipe, and inlet tube by following the instructions in
"Installing the pump head" P.80.
LC-40B X3 CL 89
4 Maintenance
4.3.2 Resetting the Delivery Amount
When PARTS MGMT TOOL is active, the delivery amount cannot be reset on the
operation panel. Reset it from PARTS MGMT TOOL.
1 Display the [MAINTENANCE]

information group of the VP function.
"3.3 Types of Screens" P.22
2 Press [↓] several times until [R SEAL USED A] is displayed.
3 Press [del].
The delivery amount of the plunger seal
before replacement is reset and the reset
date is recorded in the maintenance log.
"Displaying the delivery amount of the left/right plunger seal《L/R SEAL USED A》《L/R SEAL
USED B》" P.44
To replace the left plunger seal, display [L SEAL USED A] and press [del].
4.3.3 Check after Replacement
After replacing the plunger seal, check the following:

• Is the pumping (pressure) stable?
• Is fluid leaking from the gap between the pump head and the head holder?
• Is fluid leaking from the rinse flow line?
If the above occurs even after replacing the plunger seal, it is possible that there
are scratches on the surface of the plunger. If there are scratches on the plunger,
replace it with a new plunger.
"4.4 Cleaning/ Replacing the Plunger and Diaphragm" P.91
90 LC-40B X3 CL
4.4 Cleaning/ Replacing the Plunger and Diaphragm

A plunger and a diaphragm are attached to the left and right pump body. The following
section will explain the method for cleaning/inspecting (replacing) the plunger and the
diaphragm on the right side (when viewed from the front).
When replacing the plunger, be sure to also replace the plunger seal and the diaphragm.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Allen wrench M4 1 086-03804
Allen wrench M5 1 086-03805
Box driver 1 228-28767-91
Diaphragm jig 1 228-54852
Plunger press plate 1 228-70388
Necessary parts
4
Part Name Part Type Part No. Remark
Supplied with a
Plunger Holder Assy Consumable part 228-52069-44
diaphragm
n Prior to the inspection and the maintenancePerform the preparatory work.
4.4.1 Removing the Pump Head and the Head Holder
1 Remove the pump head, SUS pipe, and

inlet tube according to the procedure
described in "Removing the pump
head" P.76.
2 Loosen the head holder top and

bottom fittings (C and D), and remove
the rinse tube.
LC-40B X3 CL 91
4 Maintenance
3 Gradually loosen the 2 hexagonal

socket screws (B) of the head holder
(A) using an Allen wrench (M4) by
alternating between the left and right
screw.
4 Remove the head holder.
Gently pull the head holder horizontally along the plunger.

If the head holder is not removed carefully, the plunger may become damaged.
n In the case the head holder is difficult to remove
Insert into the head holder M4 bolt hole

(a) the 2 hexagonal socket screws (B) that
were removed, and tighten the left and
right sides gradually using an Allen wrench
(M4) (D).
The head holder (A) can be loosened by

pushing the pump body (C) using the 2
hexagonal socket screws (B).
92 LC-40B X3 CL
4.4.2 Cleaning and Replacement of the Plunger and the Diaphragm
When replacing the plunger, be sure to replace the plunger seal and the
diaphragms.
1 Perform the following steps to clean or inspect (replace) the plunger and
diaphragm.
1 Wipe plunger with a sheet of

wiping pape soaked with
2-propanol to remove any portion
of the seal or dirt attached to the
plunger (A).
If small pieces of the

plunger seal material
adheres to the
surface, the
airtightness cannot
be maintained.
4
2 Check that there are no visible scratches on the plunger (A) or the diaphragm (B).
• If scratches are found through visual inspection, replace with a new plunger.
Proceed to the next procedure 3.
• If no scratches are found through visual inspection, proceed to step 2.
Do NOT allow foreign particles or crystals into the head holder.

Foreign particles from the mobile phase solution or crystals from the buffer
solution getting into the head holder may cause scratches on the surface of
the plunger.
LC-40B X3 CL 93
4 Maintenance
3 Insert the box driver (B) to the

plunger holder (A), then rotate
counter-clockwise to remove the
plunger holder with the
diaphragm.
Gently insert the box driver to

the plunger holder.
The plunger may become
damaged if he box driver is
forcefuly inserted.
4 Attach a new diaphragm (B) to

the new plunger holder (A) using
the diaphragm jig (C).
Confirm the
direction of the
diaphragm when
attaching.
5 After applying the grease (black:

approximately 5 mm square size)
provided with the plunger holder
ASSY to the rear end surface of
the plunger (a), insert the plunger
holder (A) to which the
diaphragm was attached in
Procedure 4 into the pump body.
• Be careful not to get any grease on the diaphragm or the body.

• Attach the diaphragm so that it will not become detached from the
plunger holder. If the diaphragm becomes detached, wipe away any
grease and perform from Procedure 4 again.
94 LC-40B X3 CL
6 Tighten the plunger holder (A)

using the box driver (B).
7 Pinch the end of the plunger with

your two fingers, move it up,
down, right, left, clockwise, and
counterclockwise five times each
so that the grease applied to the
rear end of the plunger holder
can spread evenly. Then wipe off
the plunger surface with wiper
paper soaked in 2-propanol to clean it.
2 In the [PLUNGER SET A] screen of the control setting group, press [2] and [Enter].
(To replace the left plunger, press [1] and [Enter].)
[PUMP] on the display is highlighted and after a while stops being highlighted. The right
plunger is now at the backmost position.
"Setting the plunger stop position《PLUNGER SET A/B》" P.34
4
3 Attach the head holder to the pump
body.
Slowly push in the head

holder along the plunger.
Pushing in the head holder
forcibly may break the plunger.
4 Attach the 2 hexagonal socket screws to the head holder and tighten them with
an Allen wrench.
Tighten the right and left hexagon socket screws alternately in 90° increments.
Finally hold the longer part of the hex wrench and tighten them securely.
5 Attach rinse tubes to the top and bottom of the head holder.
LC-40B X3 CL 95
4 Maintenance
6 In the [PLUNGER SET A] screen, press [1] and [Enter].

(To replace the left plunger, press [2] and [Enter].)
plunger is now at the frontmost position.
7 Pinch the end of the plunger that is

projecting from the head holder with
your fingers and adjust its position to
make the gap (C) around the plunger
(A) inside the hole of the projection (B)
of the head holder even. While
keeping the plunger in place, wipe off
the plunger surface with clean wiper
paper soaked in 2-propanol.
Move the plunger gently.

Moving the plunger too
abruptly could scratch the
plunger surface or cause it to
break off.
8 In the [PLUNGER SET A] screen, press [2] and [Enter]. (To attach the left plunger
seal, backup ring and pump head, press [1] and [Enter].)
plunger is now at the backmost position.
When replacing the plunger, be sure to replace the plunger seal and the
diaphragms."4.3 Replacing the Plunger Seal" P.82.
9 Attach the pump head, SUS pipe, and inlet tube and set the seal rinsing kit back
in place by following the instructions in "Installing the pump head" P.80.
96 LC-40B X3 CL
4.5 Rinsing the Check Valve
4.5 Rinsing the Check Valve

This section will explain how to pump 2-propanol to rinse the check valve.
Necessary tools

Wrench (8 mm) 2 086-03002
Necessary parts
Part Name Part Type Part No. Quantity

RESISTANCE TUBE,
Replacement part 228-57908-41 1
COIL FOR CLEANING
1 After using buffer solution as the mobile phase, replace the solvent in the
reservoir bottle with water.
4
After using buffer solution as the mobile phase, do NOT replace with an
organic solvent such as 2-propanol without first replacing with water.
When using a buffer solution in the reservoir bottle, replace with distilled
water or purified water, and after pumping at least 20 mL into the instrument,
replace with new 2-propanol. If the buffer solution is directly replaced with
2-propanol, crystals will be precipitated, which can cause damage to the
plunger seal or cause problems with the check valve.
System Guide "Precautions When Replacing the Mobile Phase"
2 Replace the solvent in the reservoir bottle with 2-propanol.
3 Loosen the male nuts on the coupling

inlet and outlet with an 8-mm wrench
and then remove the coupling from
the flow path.
4 Attach the male nut (A and C) of the

column inlet and outlet that was
removed to the resistor tube (D), and
tighten it with an 8 mm wrench.
LC-40B X3 CL 97
4 Maintenance
6 Press [Enter] once.

The cursor blinks at the [FLOW] input position.
7 Press [2], [. ], [0] and [Enter].

[FLOW] is set to "2.0000" and confirmed.
8 Press [PUMP].
[PUMP] on the display is highlighted and delivery of 2-propanol starts at 2 mL/min.
Continue delivery at least 1 hour.
9 Loosen the male nut (A and C) of the

resistor tube inlet and outlet with an
8 mm wrench, and remove the resistor
tube (B) from the flow line.
10 Attach the removed male nut (A) of

the resistor tube inlet and outlet to
the coupling with an 8 mm wrench
and tighten it with the 8 mm wrench.
11 Check the pumping stability after cleaning the check valves.

"Checking the pulse《PULSE CHECK A/B》" P.49
12 Replace the solvent in reservoir bottle with the original mobile phase.
System Guide "Precautions When Replacing the Mobile Phase "
98 LC-40B X3 CL
4.6 Performing an Ultrasonic Cleaning of the Check Valve
4.6 Performing an Ultrasonic Cleaning of the Check Valve

Check valves are attached to the left and right pump heads. The following section will
explain the method for performing an ultrasonic cleaning of the check valves on the
right side (when viewed from the front).
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
1 Remove the SUS pipe and inlet tube by following the instructions in "Removing
the pump head" P.76.
2 Use a 10 mm wrench to remove the

inlet check valve (A) and the outlet
check valve (B).
4
Never disassemble the check valve.

Performance cannot be guaranteed if disassembled.
Do NOT turn the inlet check valve (A)

upside down.
If you do, the internal parts will fall out.
LC-40B X3 CL 99
4 Maintenance
3 Immerse the inlet check valve (A) and outlet check valve (B) into 2-propanol, and
clean for 5 minutes using an ultrasonic bath.
4 Rotate the inlet check valve (A) and

the outlet check valve (B) clockwise to
attach them to the pump head, and
after tightening by hand, use a 10 mm
wrench to tighten them as follows.
• Inlet check valve : Hand tightening and
120° with wrench
• Outlet check valve : Hand tightening and

60 to 90° with wrench
5 Attach SUS pipe and inlet tube by following the instructions in "Installing the
pump head" P.80.
100 LC-40B X3 CL
4.7 Replacing the Check Valve
4.7 Replacing the Check Valve

Check valves are attached to the left and right pump heads. The following section will
explain the method for Inspecting (Replacing) of the check valves.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Necessary parts

Inlet check valve ASSY 228-52964-42
Consumable parts
Outlet check valve ASSY,XR 228-53334-96
1 Check the pumping stability.

"Checking the pulse《PULSE CHECK A/B》" P.49"
4
If the result of the system check is "Pass", it is considered normal.
If the result of the system check is "Fail", perform "4.5 Rinsing the Check Valve" P.97
or "4.6 Performing an Ultrasonic Cleaning of the Check Valve" P.99, and then check the
pumping stability again. If the pumping (pressure) is still unstable, proceed to the
following procedure.
2 Remove the check valve and install a new one by following the instructions in
"4.6 Performing an Ultrasonic Cleaning of the Check Valve" P.99.
3 Attach SUS pipe and inlet tube by following the instructions in "Installing the
pump head" P.80.
4 Turn the drain valve counterclockwise to open it.
5 Press [Enter] once.

The cursor blinks at the [FLOW] input position.
6 Press [2], [. ], [0], and [Enter].

[FLOW] is set to "2.0000" and confirmed.
7 Press [PUMP] and confirm the solvent continues to flow out from the drain valve.
[PUMP] on the display is highlighted and the solvent flows out of the drain tube.
There is no problem with the valve if the solvent continues to flow out.
LC-40B X3 CL 101
4 Maintenance
4.8 Inspecting the Line Filter
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
1 Fix the line filter (A) with a 10 mm

wrench (B) and loosen the male nut (C)
of the pump outlet tube with an 8 mm
wrench (D) to remove it.
2 Pour water into a reservoir bottle and set the flow rate to 1 mL/min.
3 In a no load state, pump water at a rate of 1 mL/min.

• If the pressure is 0.3 MPa or less, it is considered normal.
• If the pressure is more than 0.3 MPa, replace the line filter.
When a mobile phase additive such as trifluoroacetic acid is used, a ghost peak
may be generated from the line filter. In this case, perform the following
procedure to rinse the line filter.
• Deliver a 17% phosphoric acid solution at a flow rate of 1 mL/min for about
30 minutes. (Pumping pressure: 1 MPa to 5 MPa)
For example, attach a stainless tube with ID0.1 x 2000mm (Part No.:
228-57908-41).
• Deliver purified water at a flow rate of 10 mL/min for about 30 minutes.
(Pumping pressure: 1 MPa to 5 MPa)
For example, attach a stainless tube with ID0.3 x 2000mm*1.
*1: Stainless tubing ID0.3 x 1000mm (Part No.: 228-53184-96) 2pc and Coupling
(Part No.: 228-16004-13) 1pc can be substituted.
102 LC-40B X3 CL
4.9 Replacing the Line Filter
4.9 Replacing the Line Filter
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Necessary parts

Line Filter Consumable part 228-57501-92

wrench (B) and loosen the male nut (C)
of the pump outlet tube with an 8 mm
wrench (D) to remove it.
2 Loosen the line filter (A) with 10 mm wrench to remove it.
3 Wipe with a cotton swab, etc., to wipe

away any dirt from the line filter port
(a).
4 Attach the new line filter (A) to the

line filter port (a) by hand, and tighten
it 45° using a 10 mm wrench.
LC-40B X3 CL 103
4 Maintenance
5 Attach the male nut (C) of the pump

outlet tube to the line filter (A) by
hand.

wrench (B) and tighten the male nut
(C) of the pump outlet tube with an
8 mm wrench (D).
When using in the front end of

LC-MS, carry out the cleaning
process described as follows to
reduce background noise. Pump
50% methanol solution at a
pressure of approx.10 MPa for
approx. 30 min. (For example,
attach a stainless steel tube
with ID 0.1 x 2000 mm (PartNo.:
228-57908-41) and adjust the
flow rate.)
4.10 Cleaning/Replacing the Suction Filter
Necessary Parts
Part Name Part Type Part No. Quantity

SUS Filter Element,
Consumable part 228-45707-91 1
w/PTFE Fitting
Use the SUCTION FILTER with tube and fittings for pump inlet (Part No. 228-45708-91) or
SUCTION FILTER with tube and fittings for the the degassing unit (Part No. 228-61905-49)
as necessary.
Contamination of suction filters can lead to contamination of mobile phase

solvents and bottles, causing background rise and ghost peaks. Even if you are
wearing gloves, do not touch the metal parts of the suction filter as much as
possible. Hold the non-metal part of the filter element.
104 LC-40B X3 CL
4.10 Cleaning/Replacing the Suction Filter
After using the buffer solution as the mobile phase, do NOT replace with an
organic solvent such as 2-propanol without first replacing with water.
When using a buffer solution, replace it with distilled water or purified water,
and after pumping at least 20 mL into the instrument, replace it with new
2-propanol. If the buffer solution is directly replaced with 2-propanol, crystals
will be precipitated, which can cause damage to the plunger seal or cause
problems with the check valve.
System Guide "Precautions When Replacing the Mobile Phase"
1 Pull the suction filter (A) from the suction tubing (B).
2 Place the suction filter into a clean container with 2-propanol, and clean it for
5 minutes using an ultrasonic bath.
3 Insert the suction tubing (B) into the

suction filter (A).
4 Replace the solvent in reservoir bottle with water, and place the suction tubing
back into the bottle.
6 Deliver water at 1 mL/min for 10 minutes.
LC-40B X3 CL 105
4 Maintenance
7 Check for air bubbles inside the suction tubing.

Replace the suction filter if air bubbles are present.
If Ghost peaking occurs, rinse the suction filter. Refer to System Guide "5.3.2
Symptoms Presumably Due to an Error, Based on a Chromatogram".
106 LC-40B X3 CL
4.11 Replacing the Drain Valve
4.11 Replacing the Drain Valve
Necessary tools

Phillips screwdriver 1 -
Necessary parts

DRAIN VALVE ASSY,TP Replacement part 228-51229-93
1 Rotate the drain valve ASSY counter-clockwise at least 3 complete rotations and
pull it off straight.
Check the hole for foreign objects.
4
2 Wet the seal portion (a) of the new
drain valve ASSY (A) with 2-propanol,
etc., and insert it into the hole.
If the drain valve ASSY is

forcefully inserted or is not
inserted straight, the seal
portion may become deformed,
resulting in leakage.
3 Tighten the drain valve ASSY

clockwise.
4 After tightening the drain valve ASSY,

attach the knob with the screw (B)
using a phillips screwdriver so that it
is vertical, and then attach the cap (C).
LC-40B X3 CL 107
4 Maintenance
4.12 Cleaning of the Leak Tray
If a leak occurred, wipe off the leak in the leak tray completely as below.
Leak of mobile phase solution used as buffer solution may dry and crystallize,
clogging the leak tray. If the leak tray is contaminated by such crystallized buffer
solution, wipe off the leak tray with wiper paper soaked in water in the same
manner as below.
1 Wipe off leak around the leak sensor or on the leak tray completely with wiper
paper.
• Do not bend or pull the leak sensor.

• If there is contamination such as crystallized buffer solution, wipe it off with
wiper paper soaked in water.
4.13 Cleaning the Exterior
4.13.1 Usual cleaning
If the exterior of the instrument is dirty, clean it with a dry soft cloth or paper wiper.
If it is very dirty, clean it as below.
1 Clean with a cloth soaked in diluted neutral detergent and wrung tightly.
2 Wipe off detergent with a cloth soaked in water and wrung tightly and then
wipe off water with a dry cloth.
Do NOT leave any part wet nor use thinner for cleaning.
Doing so may cause rust or discoloration.
108 LC-40B X3 CL
4.13 Cleaning the Exterior
4.13.2 Decontamination cleaning
After handling the hazardous substances, using the following procedure.
1 Turn off the power of the column oven.
The gas sensor may defect volatile gas generated from organic solvent.
2 Clean with a cloth soaked in Aqueous ethanol solution (80%) or aqueous sodium
hypochlorite solution (0.05%) and wrung tightly.
Do not leave metal surfaces wet.

This can cause rusting and discoloration.
LC-40B X3 CL 109
4 Maintenance
4.14 Cleaning of the Automatic Rinsing Kit
n Necessary parts

WASHING PUMP SEAL KIT Replacement part 228-70612-41
If the 10% 2-propanol water in the rinse solution bottle is dirty, replace with
new solution. If getting significantly dirty, replace with new solution once a day.
Even if it does not appear dirty, replace with new solution once a week.
1 Pour distilled water into the rinse solution botte and clean the inside of the rinse
solution bottle using a brush, etc.
2 Pour distilled water into the rinse solution bottle and rinse the inside of the
rinse solution bottle for about 5 minutes using an ultrasonic bath.
3 Pour new 10% 2-propanol water into the rinse solution bottle, and return it back
to its original positions.
4 Replace the rinse tube if the inside is dirty.
110 LC-40B X3 CL
5 Technical Information
5.1 Specifications
5.1.1 Specifications
Item Specification
Micro-volume double plunger pump
Pump Type
(approx. 10 µL/stroke)
Pumping Methods Constant flow pumping
Allowable Maximum Pressure 130 MPa
Flow Rate Setting
0.0001 to 10.0000 mL/min
Range
130 MPa (0.0001 to 3.0000 mL/min)
Maximum Delivery
80 MPa (5.0000 mL/min)
Pressure
22 MPa (10.0000 mL/min)
Constant Flow
Pumping ±1 %
(1 mL/min when water is pumped at 80 MPa,
Flow Rate Accuracy
with water and room temperature constant
between 20 to 30 °C)
No more than the large value calculated with
Flow Rate Precision
either 0.06 % RSD or 0.02 min SD
Pressure Display Accuracy Larger value of either ±2 % or ±2.4 MPa 5
Suction Filter 10 µm
Line Filter 5 µm
Commands for flow rate, pressure, [EVENT],
Time Program [LOOP] (for program repetition),
320 steps (total of 10 program files)
*1 When water is pumped at 10 to 40 MPa, with water and room temperature constant between 20
to 30 °C.
*2 When water is pumped at 40 to 60 MPa, with water and room temperature constant between 20
to 30 °C.
LC-40B X3 CL 111
5.1.2 High-Pressure Gradient System
Item Specification
Number of Solvents Mixed 2 or 3
Step, linear and exponential functions (Only
Gradient Profile when controlled by LC workstation) possible at
multiple levels
Maximum Program Steps 400 steps (total of 20 program files)
0.01 to 9999.90 minutes (in steps of 0.01
Program Duration
minutes)
Range of Set Concentrations 0 % to 100 % (in steps of 0.1 %)
±0.5 %
Concentration Accuracy*1
(1 mL/min, 80 MPa)
Flow Rate Possible 0.0001 mL/min to 10 mL/min
*1 For binary gradient with water/caffeine solution
5.1.3 Other Specifications
Item Specification
Liquid-Contacting Part
SUS316L, PEEK, ruby, sapphire, HastelloyC, polyethylene
Materials
Operating Temperature
4 °C to 35 °C
Range
Humidity Range 20 % to 85 %
Available pH Range 1 to 14
Dimensions W260 mm ´ H210 mm ´ D500 mm (Excluding protruding parts)
Mass 21 kg
Power Supply
100 V AC to 240 V AC
Voltage
* Mains supply voltage fluctuations are not to exceed 10 % of
(indicated on
the nominal supply voltage.
the instrument)
Power
Power 180 VA
Consumption
Supply
Frequency 50/60 Hz
50 A
Rated Breaking * Connect the instrument to a power outlet that is equipped
Capacity with a circuit breaker that shuts off the current at the described
value or less.
Installation Category Ⅱ, Pollution Degree 2,
Installation Environment
Altitude 2000 m max.
(IEC)
Install indoors.
Plunger Rinsing Line Automatic rinsing kit as standard equipment
Pressure Limiter
Upper/lower limits can be set
Operations
112 LC-40B X3 CL
5.1 Specifications
Item Specification
Error Display Exist (Error display and stop at the time of malfunction)
Degassing Unit 5 channels as standard equipment
LC-40B X3 CL 113
n Performance Specifications for the degassing unit
Item Specification
Number of
5 channels
Channels
When a UV absorption detector with wavelength of 210 nm and
sensitivity of 0.5 AU is used and methanol saturated with dissolved
Degassing
oxygen (HPLC grade at 25 ｰC) is flowed at a rate of 1 mL/min, UV
Performance
absorption difference with or without the degassing unit is 300 mAU or
more
10 mL/min per channel
• Assuming that the degassing capacity of He degassing of methanol as
Maximum 100 %, the capacity at the flow rate of 1 mL/min and 10 mL/min will
Operating Flow be 89 % and 25 % respectively.
Rate If more degassing capacity than the above is required, ultrasonic
vacuum degassing of the mobile phase etc. shall be carried out
beforehand.
Internal Capacity 400 μL/1 channel
Pressure ±0.1 MPa (only for suction with the solvent delivery module. It is not
Resistance possible to pressurize the degassing unit.)
A mechanism is provided that operates stably by capturing outside air
Self Purge
during vacuum pump operation
If the maximum operating flow rate is exceeded, bubbles may appear or the
degassing chamber may be overloaded and damaged.
114 LC-40B X3 CL
5.2 Maintenance Parts
5.2 Maintenance Parts
5.2.1 LC-40B X3
n Consumable Parts
No. Part Name Part No. Remarks

Seal for the pump head, with
PLUNGER SEAL,BACKUP RING UHP 228-52711-93
1 backup ring
Plunger Seal GFP 228-52711-94 Optional parts
2 Line Filter ASSY, SUS 228-57501-92
3 SUS Filter Element, w/PTFE Fitting 228-45707-91 Filter body only (cleaned)
4 Plunger ASSY 228-52069-44 With diaphragm
5 Inlet Check Valve ASSY 228-52964-42
6 Outlet Check Valve ASSY, XR 228-53334-96
LC-40B X3 CL 115

7 DIAPHRAGM 2PCS 228-55272-41
n Replacement Parts

8 PUMP HEAD 228-55230-45
9 HEAD HOLDER,2030 228-38022-01
10 Suction Filter ASSY 228-56200-48
11 FEP TUBE 3.0(O.D.)×1.5(I.D.) 670-10321-05 For the suction filter
12 DRAIN VALVE ASSY,TP 228-51229-93
13 THRUST 228-34469
14 SUS PIPE L,40-L 228-70026-01 For the left pump head
15 SUS PIPE R,40-R 228-70026-02 For the right pump head
16 TUBE HOLDER 228-70044
17 DRAIN TUBE ASSY 228-25495-93
5.2.2 Maintenance Kit
A set of consumable parts is provided as Maintenance Kit.
n Maintenance Kit for LC-40B X3 CL(Parts No. 228-53265-46)
This kit includes the parts for two pump ASSYs (one unit of LC-40B X3 CL).
Part Name Part No. Quantity Remarks

PLUNGER SEAL,BACKUP RING UHP 228-52711-93 4 With backup ring
Line Filter ASSY, SUS 228-57501-92 2
Suction Filter ASSY 228-56200-48 2
Plunger ASSY 228-52069-44 4 With diaphragm
Inlet Check Valve ASSY 228-52964-42 4
Outlet Check Valve ASSY 228-53334-96 4
116 LC-40B X3 CL
Index
A password 56
Auto-Diagnostics 24, 62 Periodic Inspection 71
Automatic Rinsing Kit 5, 110 Plunger 91
auxiliary functions screen 27 Plunger Seal 82
Available pH Range 112 Power button 6, 12
Product Information Group 44
C Purge 15
Calibration Support Information Group 55
CBM parameter 58
R
Check Valve 97, 99, 101 rinsing pump 33
Component Parts 2
Compressibility 18, 31 S
Constant Flow Delivery Mode 21 Shipping Screws 10
Consumable Parts 115 Specifications 111
[CONTROL] Setting Group 34 Status LED 11
Suction Filter 104
D System Controller 69
degassing unit 47 [SYSTEM] Setting Group 35
Diaphragm 91
Display Area 13 T
Drain Valve 107 Time Program 63
F U
[FILE] Operation Group 41 [UTILITY] Setting Group 39
G V
Gradient Mixer for High-Pressure Gradient Analysis Validation Support Information Group 48
3 volume 40
VP Functions Screen 42
H
High-Pressure Gradient System 112
L
Leak Sensor 8
Line Filter 102, 103
Liquid-Contacting Part Materials 112
M
Main Power Switch 9
Maintenance Information Group 44
Maintenance Kit 116
Maintenance Parts 115
Monitoring Screens 23
O
Operation Area 15
Operation Panel 11
Optional Parts 2
P
[PARAMETER] Setting Group 29
LC-40B X3 CL 117
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228-97198B

Solvent Delivery Module for Shimadzu

Document Name Document No. Description

Read "Safety Guideline" thoroughly before using the product.

ã 2020-2022 Shimadzu Corporation. All rights reserved.

Indications Used in This Manual

Indicates a potentially hazardous situation which, if not

Indicates a potentially hazardous situation which, if not

The following symbols are used in this manual:

Indicates an action that must not be performed.

Indicates an action that must be performed.

Indicates information provided to improve product performance.

Indicates the location of related reference information.

n EN55011 Emissions (Electromagnetic Interference)

This is a class B product. When this product causes an electromagnetic disturbance to

Operating Nexera CL System

This system is designed to perform qualitative and quantitative analysis of target

A calibration curve should be generated for each analyte in appropriate methods. At

1 Overview 1.1 Features ............................................................. 1

2.2 Behind Front Cover ............................................. 7

2.5.2 Operation Area ............................................... 15

3 Operation 3.1 Settings for the Basic Operation ......................... 17

3.1.2 Setting Flow Rate ............................................. 17

3.1.5 Setting Minimum Pressure Limit .............................. 19

3.2 Operation ........................................................ 20

3.2.2 Operation in Constant Flow Delivery Mode .................. 21

3.3 Types of Screens ................................................ 22

3.5.3 [CONTROL] Setting Group .................................... 34

3.5.4 [SYSTEM] Setting Group ...................................... 35

3.5.6 [FILE] Operation Group ........................................ 41

3.6 VP Functions Screen ........................................... 42

3.6.5 Calibration Support Information Group (CALIBRATION) ...... 55

3.7 Creating Time Program ...................................... 63

3.7.2 Time Program Setting Screen ................................. 64

3.7.3 Creating a High Pressure Gradient Program ................. 65

3.9 Connection to External Input/Output Terminals

4 Maintenance 4.1 Periodic Inspection and Maintenance .................. 71

4.1.2 List of Periodic Inspection and Maintenance ................. 73

4.1.3 Check after Inspection and Maintenance .................... 74

4.3.2 Resetting the Delivery Amount ............................... 90

4.5 Rinsing the Check Valve .................................... 97

4.13.2 Decontamination cleaning .................................. 109

4.14 Cleaning of the Automatic Rinsing Kit .............. 110

5.1.1 Specifications ............................................... 111

5.1.3 Other Specifications ......................................... 112

5.2.2 Maintenance Kit ............................................. 116

Index ......................................................... 117

n Low Flow Pulsation and Pulsation Period Enable Precise Delivery

n HIgh-pressure Flow is Available

n Automatic Plunger Rinsing

1.2 Component Parts

No. Part Name Part No. Q'ty Remark

*1 It contains cautions for use regarding the instrument.

1.3 Optional Parts

Option Part No. Features

The maximum available pressure is 44MPa.

n Gradient Mixer for High-Pressure Gradient Analysis

Part Name Part No.

Mixers described above are packaged in a set