CELL-DYN EMERALD SYSTEM

LABORATORY INFORMATION SYSTEM

INTERFACE SPECIFICATION

LIST NO. 09H40-04

REVISION F

©2010, 2020
Abbott Laboratories
Diagnostics Division
Abbott Park, IL 60064
 TABLE OF CONTENTS
TRADEMARK STATEMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
OBJECTIVE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
APPROVAL AND HISTORY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
SCOPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
TERMINOLOGY AND ABBREVIATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
SYSTEM OVERVIEW . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
GENERAL SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Physical Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
RS232 connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Ethernet connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Data Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Host serial configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Host net configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Communication Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Invoking Host Transmission . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
RUN SAMPLE Menu Automatic Transmit Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
QC RUN AND RESULTS Menu Automatic Transmit Mode . . . . . . . . . . . . . . . . . . . . . . . . . . 6
CALIBRATION Menu Automatic Transmit Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
DATALOG Menu Transmit Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Transmission Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Response from Host. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
No response from the host . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Non valid Acknowledgement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Acknowledgement with error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Frame Format. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Frame header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Frame ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Frame length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Control sum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Data representation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
FRAMES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Connection (HOST) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Connection request . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
SENDING RESULTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Sending result request . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Sending result request acknowledgement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Sending analysis result. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Result acknowledgement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Error handling in handshake mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
CALIBRATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Sending a calibration report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Calibration report acknowledgement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Calibration results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
QC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
QC Result. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Precision. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

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 Precision Result . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Control sum. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Example . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
CELL-DYN Emerald LIS Quick Reference Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42

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TRADEMARK STATEMENTS

All Abbott Laboratories product names and trademarks are owned by or licensed to Abbott
Laboratories, its subsidiaries or affiliates. No use of any Abbott trademark, trade name, trade dress, or
product name may be made without the prior written authorization of Abbott Laboratories, except to
identify the product or services of Abbott Laboratories. All other trademarks, brands, product names,
and trade names are the property of their respective companies. All rights reserved.
Except as permitted above, no license or right, express or implied, is granted to any person under any
patent, trademark, or other proprietary right of Abbott Laboratories.
CELL-DYN Emerald and related brand marks are trademarks of Abbott. Other trademarks are the
property of their respective owners.
.

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 NOTES

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1. OBJECTIVE
This document describes the data transmission format protocol between CELL-DYN Emerald
and a host Laboratory Information System (LIS).
The functionality must match the software specifications of the Emerald.

2. APPROVAL AND HISTORY

Version Date Author Reason and Type of Change

A 14/10/08 O. Genet/M. Document creation

Hepp

B 13/02/09 O. Genet/M. Updated to align with CD-Emerald Operator’s Manual

Hosseini changes.

C 11/06/10 E. Ayala Updated to align with Software Version 2.1.0.

D 6/30/11 E. Ayala Update WBC, RBC, and PLT interpretation tables

E 10/24/12 M. Wang Update to align with Software Version 2.2.0, added additional
trademark information and added a quick start guide.

F 12/02/20 N. Kallur Update section 1.2.2 Notes to align with SRS document.

3. SCOPE
This document applies to the CELL-DYN Emerald instrument. It specifies the messages and
behaviors of the initial version of the CELL-DYN Emerald Host Computer Interface.

4. TERMINOLOGY AND ABBREVIATIONS

Handshake: bi-directional communication using acknowledgement
LIS: Laboratory Information System, i.e. a HOST system
Host: external computer or data collection system
CRC: Cyclic Redundancy Code

5. SYSTEM OVERVIEW
This document describes the interface characteristics of the CELL-DYN Emerald automated
hematology analyzer when attached to a Host.

6. GENERAL SPECIFICATIONS
The Host Interface for the CELL-DYN Emerald System is a specific format designed to transfer
data to an external system. The system does not use the ASTM or CLSI (LIS-x) standards for
communications with a host system.
The format is text-oriented and is compatible with the CSV format used by various Microsoft
Office applications.
The Supervisor level password (default password is 123) is necessary to access the
COMMUNICATION menu. When LIS customization is completed, it is imperative that the user
returns the instrument to general user login (no password).

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 NOTES

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1.1 Physical Interface
1.1.1 RS232 connector
The CELL-DYN Emerald provides a standard DB-9 female connector, labelled RS232C,
mounted on the rear of the instrument.

RS232 Label
Pins on the DB-9 connector: (standard RS-232 9-pin assignments)
Pin 1: Data Carrier Detect (DCD input)
Pin 2: (output) CELL-DYN Emerald Data to Host (LIS, results)
Pin 3: (input) Host Data to CELL-DYN Emerald
Pin 4: (output) Data Terminal Ready (DTR)
Pin 5: Signal Ground
Pin 6: Data Set Ready (DSR)
Pin 7: (input) Clear to Send (CTS input) (not enabled by CELL-DYN Emerald Software)
Pin 8: (output) Request to Send (RTS output) (not enabled by CELL-DYN Emerald
Software)
Pin 9: (not connected)
1.1.2 Ethernet connector
The CELL-DYN Emerald provides an RJ 45 connector 10/100Base, labelled with the
symbol below, mounted on the rear of the instrument.

RJ 45 label

1.2 Data Interface

The system can be configured to use either TCP/IP or UDP/IP over Ethernet or RS232 in the
COMMUNICATION menu by selecting HOST SERIAL (for RS 232) or HOST NET (for TCP/IP
or UDP). The default setting is no host.
1.2.1 Host serial configuration
The system can be configured for data transmission on the RS232 channel by using the
COMMUNICATION/SERIAL PARAM. Menu. The configurable transmission parameters
include data bits, stop bits, parity and baud rate.
• The asynchronous method of data transmission (serial by bit) is used.
• All information transmitted is in character form and is represented by 8-bit or ASCII.
• Transmitted characters consist of one (1) start bit, eight (8) data bits (least
significant first), odd, even or no parity bit, and one (1) or two (2) stop bits.
• Parity may be selected as none, odd, or even. Default parity is None.
• The transmission speed may be selected from 1200, 9600, 19200, 57600 or 115200
bits per second (bps).
• The Default setting is 115200 bits per second.
• The number of bits per character is eight bits.

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 1.2.2 Host net configuration
• The system can be configured for data transmission via TCP/IP or UDP/IP over
Ethernet by using the COMMUNICATION/NET. PARAM. Menu.
• The configurable transmission parameters include protocol, host IP address, host
port, Emerald IP address, Emerald mask address, and Gateway address.
• The protocol may be set to TCP/IP in this menu. The default setting is “UDP/IP”.
• The host default IP address is 192.168.0.103. Its port is default set to 1200. Its mask
is 255.255.255.0.
• The Emerald default IP address is 192.168.0.200. The gateway default address is
192.168.0.1.
• The Emerald port is set to 3000.
• The AUTOMATON contains the configuration parameters of the Emerald.
NOTES:
• A valid IP address cannot consist of all 0’s (0.0.0.0) or all 255’s (255.255.255.255)
• Mask must be only consecutive 1 from left to right in binary
• Mask must be lower or equal to 255.255.255.252
• The mask must be in the same network class as the IP Address (class A, B or C)
• Gateway must be in the same sub-net as the Emerald.

1.3 Communication Protocol

The following is available with RS232 only.
• Transmission control is provided in one way: XOFF/XON protocol in which the Host
transmits an XOFF character (hex 13) to stop transmission from the System and an
XON character (hex 11) to re-start transmission.
• This is optional and not default-enabled. The option is available in
COMMUNICATION/SERIAL PARAM. Menu with the FLOW setting.
• The CTS/RTS pinouts are not used.
1.3.1 Invoking Host Transmission
The following assumes that the HOST mode is selected in the COMMUNICATION
menu.
1.3.1.1 RUN SAMPLE Menu Automatic Transmit Mode
The user is able to manually send the current results displayed in the RUN
SAMPLE menu by selecting the SEND button available in the TOOLS menu.
1.3.1.2 QC RUN AND RESULTS Menu Automatic Transmit Mode
The user is able to manually send all selected QC runs by selecting the SEND
button available in the TOOLS menu.
1.3.1.3 CALIBRATION Menu Automatic Transmit Mode
The user is able to manually send all selected calibration runs by selecting the
SEND button available in the TOOLS menu.
1.3.1.4 DATALOG Menu Transmit Mode
The user is able to manually send a range of selected results viewable in the
DATALOG menu or the DATE menu by selecting the SEND button available in
the TOOLS menu.

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 1.3.2 Transmission Control
1.3.2.1 Response from Host
The following assumes that the HOST mode with handshake mode is
selected in the COMMUNICATION menu.
There is no automatic re-transmission following a transmission error.
1.3.2.2 No response from the host
The instrument displays an error message “HOST: TIME OUT”.
1.3.2.3 Non valid Acknowledgement
If the acknowledgement message from the host is not the one expected, the
instrument displays an error message “HOST: SYNCHRO ERROR”
1.3.2.4 Acknowledgement with error
The host acknowledges the message with an error.
The instrument displays an error message “HOST: ACK ERROR”.
1.3.3 Frame Format
Each frame sent by the instrument consists of the following elements:
FRAME HEADER FRAME ID DATA SEGMENT
Each frame sent by the host consists of the following elements:
FRAME ID DATA SEGMENT
DATA SEGMENT is optional according to the type of frame described in section
1.4.2.3.1.
The following subsections describe these constructs in detail.
1.3.3.1 Frame header
The Emerald always begins its frame with the following header, named frame
header:
INSTRUMENT TYPE; INSTRUMENT NUMBER; INSTRUMENT
SERIAL NUMBER; USER LOGIN[CR]
The semicolon ‘;’ is the field separator. [CR] is the carriage-
return.
1.3.3.1.1 Field 1—Instrument Type
The Instrument Type is an alphanumeric field of seven (7)
characters: enclosed in double quotation marks. The string for
the CELL-DYN Emerald is sent as “”EMERALD””.
1.3.3.1.2 Field 2—Instrument Number
The Instrument Number is a numeric field of up to two (2)
characters ranging from 0 to 99.
This number is set in the COMMUNICATION menu. The default
value is 1.
1.3.3.1.3 Field 3—Instrument Serial Number
The Instrument Serial Number is an alphanumeric field of thirteen
(13) characters including the dash (-) character.

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 1.3.3.1.4 Field 4—User Login
The User Login is a field of up to ten (10) characters UTF8
encoded.
1.3.3.2 Frame ID
The frame ID allows to distinguish the type of information transmitted.

FRAME ID SENDER INFORMATION

INSTRUMENT HOST (with handshake

enabled)

CONNECT X X Connection test request

ACK_CONNECT X Connection acknowledged

NAK_CONNECT X Connection acknowledged with

failed status

RESULT_READY X Instrument ready to send result

data

RESULT X Result data being sent

ACK_RESULT_READY X Host acknowledge: ready for

result data

ACK_RESULT X Host acknowledge: end of result

data receipt

CALIBRATION X Calibration report being sent

ACK_CALI X Host acknowledge: end of

calibration report receipt

1.3.3.3 Frame length

The length is variable.
1.3.3.4 Control sum
A CRC 16 is provided according to the type of frame and may be optionally
processed by the host to verify correct transmission.
Details of this algorithm are available in section 1.5.1.
1.3.3.5 Data representation
Numeric data are transmitted in fields of variable length. A data segment is
made of several data fields.
1.3.3.5.1 Data field
Each data sent by the instrument consists of the following
elements:
FIELD ID FIELD SEPARATOR DATA VALUE [CR]

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 According to the type of field, several data values, semicolon separated, may follow a
field ID:
• The decimal separator is the dot ‘.’.
• The fields separator is the semicolon ‘;’.
• The lines separator and the end of frame indicator are the carriage return [CR].
• All field IDs must be transmitted. If no data value is available, the data value field is
empty.
• There are two exceptions mentioned in the analysis result concerning the PDW and
PCT values.
All Identifier key words (frame, parameter) are in upper case.
The following fields are UTF8 encoded: SID, PID, ID, blood type (specimen), operator,
lot numbers.

1.4 FRAMES
1.4.1 Connection (HOST)
The Connection frame is used to determine if the connection between the CELL-DYN
Emerald and the host computer is operational.
This frame is not mandatory to establish an operational connection with the host.
This transaction may be initiated either by the instrument or the host.
1.4.1.1 Connection request
1.4.1.1.1 Initiated by the Emerald
The Emerald sends this request when a user logs in.

Emerald Host

Connection request FRAME HEADER[CR]

CONNECT;200207-000451;7[CR]

ACK CONNECT;7 Acknowledge

Or NAK_CONNECT;7

FRAME HEADER[CR]
CONNECT; instrument serial number; format version[CR]

Please refer to section 1.3.3.1 for details.

The current format version is number 7.
1.4.1.1.2 Initiated by the Host

Host Emerald

Connection request CONNECT;7[CR]

FRAME HEADER[CR] Acknowledge

CONNECT;200207-000451;5[CR]

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 1.4.2 SENDING RESULTS
The Emerald sends results after the Host acknowledgement occurs, in order to avoid
sending a data stream when there is no host connected.

Emerald Host

Sending result requested FRAME HEADER[CR]

RESULT_READY;SIZE_FIELD[CR]

ACK_RESULT_READY[CR] Sending result request

Acknowledge

Analysis result sending FRAME HEADER[CR]

RESULT[CR]RESULT DATA SEGMENT

ACK_RESULT;OK[CR] Result acknowledge

1.4.2.1 Sending result request

FRAME HEADER[CR]
RESULT_READY;Size_field[CR]
1.4.2.1.1 Field 1—Size
The size is a numeric field that is the size of the data segment to
be sent by the instrument with a value ranging from 0 to
4294967295(2^32-1).
1.4.2.2 Sending result request acknowledgement
ACK_RESULT_READY[CR]
1.4.2.3 Sending analysis result
FRAME HEADER[CR]
RESULT[CR]

1.4.2.3.1 Result data segment

The following fields are described based upon their rank order in
the data segment.
1.4.2.3.1.1 Field 1 - Date
The Specimen Date, giving the date on which the
specimen was run, is an alphanumeric field of ten (10)
characters. The date format is DD/MM/YYYY.
Field ID Field ID length Data Format Length

DATE 4 DD/MM/YYYY 10

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 1.4.2.3.1.2 Field 2 - Time
The Specimen Time is an alphanumeric field of eight (8)
characters. It gives the time at which the specimen was
run in standard 24-hour format.
Field ID Field ID length Data Format Length

TIME 4 HH:MM:SS 8

1.4.2.3.1.3 Field 3 - Mode

The mode field is an alphanumeric field of up to thirteen
(13) characters. It gives the analysis mode: standard
analysis run, QC run, precision run, calibration run,
linearity run or startup run.
Field ID Field ID length Data format Length

MODE 4 NORMAL Variable

1.4.2.3.1.4 Field 4 - Unit

The unit code is a numeric field of one (1) character with
a value ranging from 1 to 3.
The parameters reported by the instrument may be
represented in any of three different sets of measurement
units as follows:
Unit code value Unit system

1 USA = standard

2 S.I.

3 S.I. MOD

Field ID Field ID length Data format Length

UNIT 4 Unit code value 1

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 Following are the unit labels according to the selected unit set:
UNITS

Parameters USA(standard) SI SI MOD

WBC XXX.X 103/µL XXX.X 109/L XXX.X 109/L

RBC XX.XX 106/µL XX.XX 1012/L XX.XX 1012/L

HGB XX.X g/dL XXX g/L XX.XX mmol/L

HCT XX.X % X.XXX L/L X.XXX L/L

MCV XXX.X fL XXX.X fL XXX.X fL

MCH XX.X pg XX.X pg X.XX fmol

MCHC XX.X g/dL XXX g/L XX.XX mmol/L

RDW XX.X % XX.X %CV XX.X %CV

PLT XXXX 103/µL XXXX 109/L XXXX 109/L

MPV XX.X fL XX.X fL XX.X fL

PCT* X.XXX % X.XXX mL/L X.XXX mL/L

PDW* XX.X % XX.X % XX.X %

1.4.2.3.1.5 Field 5 - Seq

The sequence number is a numeric field of four (4)
characters with values ranging from 1 to 9999.
Field ID Field ID length Data format Length

SEQ 4 Integer 1 up to 4

1.4.2.3.1.6 Field 6 - SID

The Sample ID is an alphanumeric field of up to sixteen
(16) characters, UTF8 encoded. A SID is mandatory to
run an analysis run.
Field ID Field ID length Data format Length

SID 3 Alphanumeric, UTF8 encoded 1 up to 16

1.4.2.3.1.7 Field 7 - PID

The Patient ID is an alphanumeric field of up to sixteen
(16) characters, UTF8 encoded.
Field ID Field ID length Data format Length

PID 3 Alphanumeric, UTF8 encoded 0 up to 16

* Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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 1.4.2.3.1.8 Field 8 - ID
The ID is an alphanumeric field of up to twenty (20)
characters, UTF8 encoded. It is the patient name.
In Linearity mode, this field is forced to Linearity.
Field ID Field ID length Data format Length

ID 2 Alphanumeric, UTF8 encoded 0 up to 20

1.4.2.3.1.9 Field 9 - Type

The Type is an alphanumeric field of up to ten (10)
characters, UTF8 encoded. It is the specimen name.
Field ID Field ID length Data format Length

TYPE 3 Alphanumeric, UTF8 encoded 1 up to 10

There are 20 specimen types that can be defined in the

instrument software. The first (default) one, named
STANDARD, cannot be modified.
1.4.2.3.1.10 Field 10 - Test
Test is an alphanumeric field of three (3) characters. It is
set to LMG for the type of tests runs on this instrument.
Field ID Field ID length Data format Length

TEST 4 LMG 3

1.4.2.3.1.11 Field 11 - Operator

The Operator is an alphanumeric field of up to ten (10)
characters.
If no Operator ID is specified, the field is empty.
Field ID Field ID length Data format Length

OPERATOR 8 Alphanumeric, UTF8 encoded 0 up to 10

1.4.2.3.1.12 Field 12 - WBC

NOTE:
• To better understand flagging fields, refer to Section 3 in the CELL-DYN Emerald
System Operator’s Manual.
• The count values are transmitted to the host in the selected unit.
• For Rank 3: ‘*’ is used for L1 and/or L5 WBC flags and ‘s’ is used for L2 and/or L3
WBC flags. If both flags are generated simultaneously, ‘*’ will supercede ‘s’.

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 Rank Data values Data format Length

1 Field ID WBC 3

2 Count value Numeric Variable (refer to the units table). Maximum 5

+++++ 5
----- 5

3 Suspect flag Empty, ‘s’ or ‘*’ Up to 1 character

4 Flag: over-range Empty or ‘D’ or ‘L’ Up to 1 character

or panic flags or ‘l’ or ‘h’ or ‘H’

5 Low Panic value Numeric Variable (refer to the units table)

6 Low value Numeric Variable (refer to the units table)

7 High value Numeric Variable (refer to the units table)

8 High panic value Numeric Variable (refer to the units table)

For the data values at rank 2, 5, 6, 7 and 8, please refer to the following table to
determine the maximum length:
UNITS

Parameters USA(standard) SI SI MOD

WBC XXX.X XXX.X XXX.X

RBC XX.XX XX.XX XX.XX

HGB XX.X XXX XX.XX

HCT XX.X X.XXX X.XXX

MCV XXX.X XXX.X XXX.X

MCH XX.X XX.X X.XX

MCHC XX.X XXX XX.XX

RDW XX.X XX.X XX.X

PLT XXXX XXXX XXXX

MPV XX.X XX.X XX.X

PCT* X.XXX X.XXX X.XXX

PDW* XX.X XX.X XX.X

* Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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 1.4.2.3.1.13 Field 13 - RBC
Please refer to the WBC field description for details. The
field ID is RBC.
1.4.2.3.1.14 Field 14 - HGB
Please refer to the WBC field description for details. The
field ID is HGB.
1.4.2.3.1.15 Field 15 - HCT
Please refer to the WBC field description for details. The
field ID is HCT.
1.4.2.3.1.16 Field 16 - MCV
Please refer to the WBC field description for details. The
field ID is MCV.
1.4.2.3.1.17 Field 17 - MCH
Please refer to the WBC field description for details. The
field ID is MCH.
1.4.2.3.1.18 Field 18 - MCHC
Please refer to the WBC field description for details. The
field ID is MCHC.
1.4.2.3.1.19 Field 19 - RDW
Please refer to the WBC field description for details. The
field ID is RDW.
1.4.2.3.1.20 Field 20 - PLT
Please refer to the WBC field description for details. The
field ID is PLT.
1.4.2.3.1.21 Field 21 - MPV
Please refer to the WBC field description for details. The
field ID is MPV.
1.4.2.3.1.22 Field 22 - PCT*
Please refer to the WBC field description for details. The
field ID is PCT.
1.4.2.3.1.23 Field 23 - PDW*
Please refer to the WBC field description for details. The
field is PDW.
1.4.2.3.1.24 Field 24 - LYM%
Please refer to the WBC field description for details. The
field is LYM%.

*Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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9159926A—December 2020
 1.4.2.3.1.25 Field 25 - MID%
Please refer to the WBC field description for details. The
field is MID%.
1.4.2.3.1.26 Field 26 - GRA%
Please refer to the WBC field description for details. The
field is GRA%.
1.4.2.3.1.27 Field 27 - LYM
Please refer to the WBC field description for details. The
field is LYM.
1.4.2.3.1.28 Field 28 - MID
Please refer to the WBC field description for details. The
field is MID.
1.4.2.3.1.29 Field 29 - GRA
Please refer to the WBC field description for details. The
field is GRA.
1.4.2.3.1.30 Field 30 - WBC channel data
Following the field ID, there are 128 values. Each value is
followed by a ‘;’ separator and with a value ranging from 0
to 255.
Field ID Field ID length Data format Length

WBC CURVE 9 Numeric integer Variable

1.4.2.3.1.31 Field 31 - WBC thresholds

This field gives the two WBC thresholds M1 and M2 set
on the system. Each is a value ranging from 0 to 255.
Rank Data values Data format Length

1 Field ID WBC THRESHOLDS 14

2 Threshold M1 Numeric Variable. Integer ranging from 0 to 255

3 Threshold M2 Numeric Variable. Integer ranging from 0 to 255

4 Not used 0 1 character

1.4.2.3.1.32 Field 32 - RBC channel data

Following the field ID, there are 128 values. Each value is
followed by a ‘;’ separator and with a value ranging from 0
to 255.
Field ID Field ID length Data Format Length

RBC CURVE 9 Numeric integer Variable

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 1.4.2.3.1.33 Field 33 - RBC thresholds
This field gives both RBC thresholds that are unused on
the Emerald. So, these values have no meaning on the
system.

Rank Data values Data Format Length

1 Field ID RBC THRESHOLDS 14

2 Threshold M1 32 2

3 Threshold M2 55 2

1.4.2.3.1.34 Field 34 - PLT channel data

Following the field ID, there are 128 values. Each value is
followed by a ‘;’ separator and with a value ranging from 0
to 255.
1.4.2.3.1.35 Field 35 - PLT thresholds
This field gives the PLT thresholds set on the system. It is
a value ranging from 0 to 255.
Rank Data values Data Format Length

1 Field ID PLT THRESHOLDS 14

2 Threshold P Numeric Variable

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 1.4.2.3.1.36 Field 36 - Alarms
This field lists the instrument alarms, operational alerts
and measurand data flags applied on a result.
NOTE: To better understand Flagging fields, refer to Section 3 in
the CELL-DYN Emerald System Operator’s Manual.
Each flag is present in the list only if the flag is set. It
means that the according data values are available if the
according alarm flag is set. So, the number of data values
depends on the number of flags set.
Rank Data values Data Format Length

1 Field ID ALARMS 6

2 L1 L1 2

3 L2 L2 2

4 L3 L3 2

5 L5 L5 2

6 P1 P1 2

7 P2 P2 2

8 P3 P3 2

9 Startup not done S-UP NOT DONE 13

10 Startup failed S-UP FAIL 9

11 QC not done QC NOT DONE 11

12 QC failed QC FAIL 7

13 Temperature alert INS-T 5

14 Pressure alert INS-P 5

15 HGB channel INS-H 5

saturation

16 WBC clog W_CL 4

17 RBC clog R_CL 4

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 1.4.2.3.1.37 Field 37 - WBC interpretive result
This field lists the WBC interpretive result.
NOTE: To better understand WBC interpretive results, refer to
Section 3 in the CELL-DYN Emerald System Operator's
Manual.
Each WBC interpretive result is present in the list only if it
is set. This means that the according data values are
available if the according interpretive flag is set. So, the
number of data values depends on the number of
interpretive flags set.
rank Data values Data Format Length

1 Field ID INTERPRETIVE_WBC 16

2 Leukocytosis LEU> 4

3 Leukopenia LEU< 4

4 Lymphocytosis LYM> 4

5 Lymphopenia LYM< 4

6 Granulocytosis GRA> 4

7 Granulocytopenia GRA< 4

8 Unable to interpret NO_INTERPRETATION 17

1.4.2.3.1.38 Field 38 - RBC interpretive result

This field lists RBC interpretive results.
NOTE: To better understand RBC interpretive results, refer to
Section 3 in the CELL-DYN Emerald System Operator’s
Manual.
Each RBC interpretive result is present in the list only if it
is set. This means that the according data values are
available if the according interpretive flag is set. So, the
number of data values depends on the number of
interpretive flags set.
Rank Data values Data Format Length

1 Field ID INTERPRETIVE_RBC 16

2 Anemia ANE 3

3 Erythrocytosis ERY> 4

4 Macrocytosis MACRO 5

5 Microcytosis MICRO 5

6 Unable to interpret NO_INTERPRETATION 17

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 1.4.2.3.1.39 Field 39 - PLT interpretive result
This field lists PLT interpretive results.
NOTE: To better understand PLT interpretive results, refer to
Section 3 in the CELL-DYN Emerald System Operator’s
Manual.
Each PLT interpretive result is present in the list only if it
is set. This means that the according data values are
available if the according interpretive flag is set. So, the
number of data values depends on the number of
interpretive flags set.

Rank Data values Data Format Length

1 Field ID INTERPRETIVE_PLT 16

2 Thrombocytosis THR> 4

3 Thrombocytopenia THR< 4

4 Giant platelets GIANTP 6

5 Cell debris CELLD 5

6 Platelet aggregates PLTAGGR 7

7 Unable to interpret NO_INTERPRETATION 17

1.4.2.3.1.40 Field 40 - COMMENT

This field is filled with text if IUO** mode and US** mode
are enabled. Both options are default disabled.
Field ID Field ID length Data Format Length

COMMENT 7 PCT* and PDW* are for Info Only 29

1.4.2.3.1.41 Field 41 - END RESULT

This field is populated with the CRC value computed on
the transmitted buffer and is the end marker of the result
transmission.
The Algorithm is described in section 1.5.
Field ID Field ID length Data Format Length

END RESULT 10 Integer Variable

* Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S.
** These Options may only be selected outside of the U.S.

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 1.4.2.3.2 Result data segment example
DATE;06/06/2008[CR]
TIME;13:41:29[CR]
MODE;NORMAL[CR]
UNIT;1[CR]
SEQ;31;0[CR]SID;No ID Entered[CR]
PID;[CR]
ID;[CR]
TYPE;STANDARD[CR]
TEST;LMG[CR]
OPERATOR;OG[CR]
WBC;12.0 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
RBC;5.20 ;;H;0.00 ;0.00 ;0.00 ;0.00 [CR]
HGB;11.9;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
HCT;40.9;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MCV;78.7 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MCH;22.9 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MCHC;29.1 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
RDW;17.7 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
PLT;220 ;;H;0 ;0 ;0 ;0 [CR]
MPV;7.6 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
LYM%;22.5;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MID%;23.7;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
GRA%;53.8;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
LYM;2.7 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MID;2.8 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
GRA;6.5 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
WBC
CURVE;0;0;0;0;0;0;0;0;1;5;13;25;40;56;73;91;109;128;
146;165;184;202;220;235;244;245;240;229;215;199;182;
164;145;127;108;89;71;54;40;31;25;21;18;17;17;17;17;
17;17;18;20;23;25;27;29;32;35;37;39;41;44;47;49;51;
53;56;59;61;63;65;68;71;73;75;77;79;82;84;86;88;91;
94;96;98;98;98;97;95;94;92;89;87;85;83;81;78;75;73;
71;69;66;63;61;59;57;54;51;49;47;45;42;39;37;35;33;
30;27;23;17;11;6;2;0;0;0;0;0;0;[CR]
WBC THRESHOLDS;25;37;0;[CR]
RBC
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;1;3;6;12;20;30;42;56;70;86;102;119;136;
153;170;186;201;213;221;225;225;221;214;204;192;178;
163;147;130;113;96;80;64;50;37;27;19;13;8;5;3;1;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;[CR]
RBC THRESHOLDS;32;55
[CR]PLT
CURVE;0;0;0;0;0;0;1;2;4;7;10;14;19;23;29;34;40;46;
52;58;64;70;77;83;89;95;100;104;108;110;112;113;114;
113;113;112;111;109;107;105;103;101;98;96;94;91;89;

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 86;83;81;78;75;73;70;68;65;63;60;57;55;52;49;47;44;
41;39;36;33;31;28;25;23;20;17;15;12;10;8;6;5;4;3;2;
1;1;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;[CR]
PLT THRESHOLDS;100[CR]
ALARMS;QC FAIL;INS-T;[CR]
INTERPRETIVE_WBC;LEU>;LYM>;GRA>;[CR]
INTERPRETIVE_RBC;ERY>;MACRO;[CR]
INTERPRETIVE_PLT;THR>;GIANTP;[CR]
COMMENT;;[CR]
END_RESULT;7774[CR]
1.4.2.4 Result acknowledgement
ACK_RESULT;A;B[CR]
Where A : OK or error code, B : Nothing.
Regardless of the error code transmitted by the host, the CELL-DYN Emerald
does not interpret the error code, so if A is different from OK, the instrument
generates an error such as described in section.1.3.2.4.
1.4.2.5 Error handling in handshake mode
In handshake mode, each result is tagged as sent if no error is generated in
the acknowledgement process. If an error is ever generated then the result is
not tagged as sent.
At next login, the CELL-DYN Emerald prompts the user to automatically
transmit the results not sent. The user then has the ability to accept, to
postpone or ignore (in this case the results are flagged as sent).
In non handshake mode, the CELL-DYN Emerald assumes that all frames
were correctly sent and so marks all samples as ‘transmitted’.

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9159926A—December 2020
 1.4.3 CALIBRATION

Emerald Host

Sending calibration report FRAME HEADER[CR]

CALIBRATION;DATA SEGMENT

ACK_CALI;LOT NAME;OK[CR] calibration report

acknowledge

Calibration results sending: FRAME HEADER[CR]

RESULT_READY;SIZE_FIELD [CR]

Sending calibration result ACK_RESULT_OK[CR] Sending calibration

request result request
acknowledge report
Calibration result sending: FRAME HEADER[CR]
RESULT[CR]RESULT SEGMENT
Calibration Result
ACK_RESULT;OK[CR] acknowledge

1.4.3.1 Sending a calibration report

FRAME HEADER[CR]
CALIBRATION;CALIBRATION DATA SEGMENT
The following fields are described based on their rank order in the calibration
data segment.
1.4.3.1.1 Field 1 - Calibration field ID - data segment
Rank Data values Data Format Length

1 Field ID CALIBRATION 11

2 User login Alphanumeric Up to 10 characters. UTF8 encoded

3 Calibration Date DD/MM/YYYY 10

4 Calibration Time HH:MM:SS 8

5 Lot Name Alphanumeric Up to 10 characters. UTF8 encoded

6 Expiry Date DD/MM/YYYY 10

7 Creation Date DD/MM/YYYY 10

8 Creation Time HH:MM:SS 8

10 WBC calibration factor numeric Float ranging from 0.5 to 2.0

11 RBC calibration factor numeric Float ranging from 0.5 to 2.0

12 HGB calibration factor numeric Float ranging from 0.5 to 2.0

13 MCV calibration factor numeric Float ranging from 0.5 to 2.0

14 PLT calibration factor numeric Float ranging from 0.5 to 2.0

15 Number of result numeric Integer ranging from 0 to 10

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 1.4.3.1.1.1 User Login
The User Login, which identifies the operator that
performed the calibration, is an alphanumeric field of up
to ten (10) characters. It is UTF8 encoded.
1.4.3.1.1.2 Calibration Date
The Calibration Date, the date on which the calibration
was done, is an alphanumeric field of ten (10) characters.
The default format of the date is DD/MM/YYYY.
1.4.3.1.1.3 Calibration Time
The Calibration Time is an alphanumeric field of eight (8)
characters. It gives the time at which the Calibration was
done in standard 24-hour format.
1.4.3.1.1.4 Lot name
The Calibration Lot is an alphanumeric field of up to eight
(8) characters.
1.4.3.1.1.5 Expiry Date
The Expiry Date, the expiration date of the calibrator lot,
is an alphanumeric field of ten (10) characters.The default
format of the date is DD/MM/YYYY.
1.4.3.1.1.6 Creation Date
The Creation Date, the date on which the calibration was
done, is an alphanumeric field of ten (10) characters. The
default format of the date is DD/MM/YYYY.
1.4.3.1.1.7 Creation Time Field
The Creation Time is an alphanumeric field of eight (8)
characters. It gives the time at which the Calibration was
done in standard 24-hour format.
1.4.3.1.1.8 WBC calibration factor
The WBC calibration factor is a numeric field filled out
with the computed calibration factor. It ranges from 0.5 to
2.0.
1.4.3.1.1.9 RBC calibration factor
The RBC calibration factor is a numeric field filled out with
the computed calibration factor. It ranges from 0.5 to 2.0.
1.4.3.1.1.10 HGB calibration factor
The HGB calibration factor is a numeric field filled out with
the computed calibration factor. It ranges from 0.5 to 2.0.
1.4.3.1.1.11 MCV calibration factor
The MCV calibration factor is a numeric field filled out
with the computed calibration factor. It ranges from 0.5 to
2.0.

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9159926A—December 2020
 1.4.3.1.1.12 PLT calibration factor
The PLT calibration factor is a numeric field filled out with
the computed calibration factor. It ranges from 0.5 to 2.0.
1.4.3.1.1.13 Number of result
When a manual calibration is performed, the number of
results is set to 0.
1.4.3.1.2 Field 2 - WBC target assay / limit
This field lists the assay value and limits input from the
calibrator’s assay sheet for the WBC parameter.

Rank Data values Data Format Length

1 Field ID WBC 3

2 WBC assay value Numeric Float

WBC limit Numeric Float

1.4.3.1.3 Field 3 - RBC target value /limits

This field lists the assay value and limits input from the
calibrator’s assay sheet for the RBC parameter.

Rank Data values Data Format Length

1 Field ID RBC 3

2 RBC assay value Numeric Float

3 RBC limit Numeric Float

1.4.3.1.4 Field 4 - HGB target value / limits

This field lists the assay value and limits input from the
calibrator’s assay sheet for the HGB parameter.

Rank Data values Data Format Length

1 Field ID HGB 3

2 HGB assay value Numeric Float

3 HGB limit Numeric Float

1.4.3.1.5 Field 5 - MCV target value / limits

This field lists the assay value and limits input from the
calibrator’s assay sheet for the MCV parameter.

Rank Data values Data Format Length

1 Field ID MCV 3

2 MCV assay value Numeric Float

3 MCV limit Numeric Float

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9159926A—December 2020
 1.4.3.1.6 Field 6 - PLT target value / limits
This field lists the assay value and limits input from the
calibrator’s assay sheet for the PLT parameter.

Rank Data values Data Format Length

1 Field ID PLT 3

2 PLT assay value Numeric Float

3 PLT limit Numeric Float

1.4.3.1.7 Field 7 - END CALI

This field is populated with the CRC value computed on the
transmitted buffer and is the end marker of the calibration report
transmission.
The Algorithm is described in section 1.5

Field ID Field ID length Data Format Length

END CALI 8 Integer Variable

1.4.3.1.8 Calibration data segment example

CALIBRATION;OG;20/06/2008;15:02:08;CALI;01/01/
2009;20/06/
2008;14:54:30;OG;0.556000;1.000000;1.000000;0.922000
;1.000000;2[CR]
WBC; 5.0; 2.0[CR]
RBC; 6.00; 2.00[CR]
HGB;25.0; 5.0[CR]
MCV; 80.0; 20.0[CR]
PLT; 150; 10[CR]
END_CALI;8333[CR] ...
1.4.3.2 Calibration report acknowledgement
Calibration acknowledgement by the host is done by the following frame:
ACK_CALI;Calibration Lot;A[CR]
Where A : OK or Error code.
1.4.3.3 Calibration results
The runs used for the calibration are sent after the calibration report. The
number of calibration results is given in the calibration field.
The calibration result segment is a subset of the fields described in the
analysis result segment with some specific details listed below.
1.4.3.3.1 Calibration result segment
1.4.3.3.1.1 Field 1 - Date
The Date, the date on which the calibration run was done,
is an alphanumeric field of ten (10) characters. The
default format of the date is DD/MM/YYYY.

Field ID Field ID length Data Format Length

DATE 4 DD/MM/YYYY 10

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 1.4.3.3.1.2 Field 2 - Time
The Time is an alphanumeric field of eight (8) characters.
It gives the time at which the calibration run was done in
standard 24-hour format.

Field ID Field ID length Data Format Length

TIME 4 HH:MM:SS 8

1.4.3.3.1.3 Field 3 - Mode

The mode field is an alphanumeric field of up to thirteen
(13) characters. It gives the analysis mode, which is here
CALIBRATION.

Field ID Field ID length Data Format Length

MODE 4 CALIBRATION 11

1.4.3.3.1.4 Field 4 - Unit

Please refer to Section 1.4.2.3.1.4
1.4.3.3.1.5 Field 5 - Seq
Please refer to Section 1.4.2.3.1.5
1.4.3.3.1.6 Field 6 - Test
Please refer to Section 1.4.2.3.1.10
1.4.3.3.1.7 Field 7 - Operator
Please refer to Section 1.4.2.3.1.11
1.4.3.3.1.8 Field 8 - WBC
NOTE: To better understand Flagging fields, refer to Section 3 in
the CELL-DYN Emerald System Operator’s Manual.
• The count values transmitted to the host are sent in the selected units.
• For Rank 3: ‘*’ is used for L1 and/or L5 WBC flags and ‘s’ is used for L2 and/or L3
WBC flags. If both flags are generated simultaneously, ‘*’ will supercede ‘s’.

Rank Data values Data Format Length

1 Field ID WBC 3

2 Count value Numeric Variable (look at the units table). Maximum 5

+++++ 5
----- 5

3 Suspect flag Empty, ‘s’ or ‘*’ Up to 1 character

4 Flag: over range or Empty or ‘D’ or Up to 1 character

panic flags ‘L’ or ‘l’ or ‘h’ or
‘H’

5 Low Panic value Empty 0

6 Low value Empty 0

7 High value Empty 0

8 High panic value Empty 0

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 1.4.3.3.1.9 Field 9 - RBC
Please refer to the WBC field description for details. The
field ID is “RBC”.
1.4.3.3.1.10 Field 10 - HGB
Please refer to the WBC field description for details. The
field ID is “HGB”.
1.4.3.3.1.11 Field 11 - MCV
Please refer to the WBC field description for details. The
field ID is “MCV”.
1.4.3.3.1.12 Field 12 - PLT
Please refer to the WBC field description for details. The
field ID is “PLT”.
1.4.3.3.1.13 Field 13 - END RESULT
Please refer to Section 1.4.2.3.1.41
1.4.3.3.2 Calibration result segment example
RESULT[CR]
DATE;20/06/2008[CR]
TIME;15:00:24[CR]
MODE;CALIBRATION[CR]
UNIT;1[CR]
SEQ;1;0[CR]
TEST;LMG[CR]
OPERATOR;OG[CR]
WBC;7.0 ;;;;;;[CR]
RBC;4.70 ;;;;;;[CR]
HGB;11.4;;L;;;;[CR]
MCV;86.0 ;;;;;;[CR]
PLT;120 ;;L;;;;[CR]
END_RESULT;11687[CR] ..

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9159926A—December 2020
 1.4.4 QC

Emerald Host
Sending QC result FRAME HEADER[CR]
request RESULT_READY;1280[CR]

ACK_RESULT_READY[CR] Sending QC result

request acknowledge

QC result sending FRAME HEADER[CR]

RESULT[CR]RESULT DATA SEGMENT
QC Result
ACK_RESULT;OK[CR] acknowledge

1.4.4.1 QC Result
The QC result segment is a subset of the fields described in the analysis
result segment with some specific details listed below.
1.4.4.1.1 QC Result segment
1.4.4.1.1.1 Field 1 - Date
The Date, the date on which the QC run was done, is an
alphanumeric field of ten (10) characters. The default format of
the date is DD/MM/YYYY.

Field ID Field ID length Data Format Length

DATE 4 DD/MM/YYYY 10

1.4.4.1.1.2 Field 2 - Time

The Time is an alphanumeric field of eight (8) characters.
It gives the time at which the QC run was done in
standard 24-hour format.

Field ID Field ID length Data Format Length

TIME 4 HH:MM:SS 8

1.4.4.1.1.3 Field 3 - Mode

The mode field is an alphanumeric field of up to thirteen
(13) characters. It gives the analysis mode: here QC.

Field ID Field ID length Data Format Length

MODE 4 QC 2

1.4.4.1.1.4 Field 4 - Unit

Please refer to Section 1.4.2.3.1.4

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 1.4.4.1.1.5 Field 5 - Seq
Please refer to Section 1.4.2.3.1.5
Note that in this case the SEQ field is filled out with the
sequence number initialized by the CELL-DYN Emerald
when the QC run is requested.
If the QC results are sent later, then the sequence
number becomes a rank number from 0 to n.
1.4.4.1.1.6 Field 6 - Lot Name
The Lot Name giving the name of the QC lot is an
alphanumeric field of up to eight (8) characters, UTF8
encoded.

Field ID Field ID length Data Format Length

LOT 3 Alphanumeric From 1 up to 8

1.4.4.1.1.7 Field 7 - Level

The Lot Level, giving the level of the QC, is an
alphanumeric field of one (1) character.

Field ID Field ID length Data Format Length

LEVEL 5 H or L or N 1

H for High Level

L for Low Level
N for Normal Level
1.4.4.1.1.8 Field 8 - Lot Date
The Lot Date, giving the date and time on which the QC
lot was created.

rank Fields Data Format Length

1 Field ID LOT DATE 8

2 Creation date DD/MM/YYYY 10

3 Creation time HH:MM:SS 8

1.4.4.1.1.9 Field 9 - Expiry DateType

The Expiry Date, the expiration date of the QC lot, is an
alphanumeric field of ten (10) characters. The default
format of the date is DD/MM/YYYY

Field ID Field ID length Data Format Length

EXPIRY DATE 11 DD/MM/YYYY 10

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 1.4.4.1.1.10 Field 10 - User
The User, the operator who created the QC lot, is an
alphanumeric field of up to ten (10) characters. It is UTF8
encoded.

Field ID Field ID length Data Format Length

USER 4 Alphanumeric Up to 10. UTF8 encoded.

1.4.4.1.1.11 Field 11 - Test

Please refer to Section 1.4.2.3.1.10
1.4.4.1.1.12 Field 12 - Operator
Please refer to Section 1.4.2.3.1.11
1.4.4.1.1.13 Field 13 - WBC
NOTE: To better understand Flagging fields, refer to Section 3 in
the CELL-DYN Emerald System Operator’s Manual.
•The count values transmitted to the host are sent in the
selected unit.
•For Rank 3: ‘*’ is used for L1 and/or L5 WBC flags and ‘s’
is used for L2 and/or L3 WBC flags. If both flags are
generated simultaneously, ‘*’ will supercede ‘s’.

rank Data values Data Format Length

1 Field ID WBC 3

2 Count value Numeric Variable (refer to the units table). Maximum 5

+++++ 5
----- 5

3 Suspect flag Empty, ‘s’ or ‘*’ Up to 1 character

4 Flag: over range or Empty or ‘D’ or ‘L’ Up to 1 character

panic flags or ‘l’ or ‘h’ or ‘H’

5 Low Panic value Numeric Variable (refer to the units table)

In QC, the value here is the low limit assay value.

6 Low value Empty 0

7 High value Empty 0

8 High panic value Numeric Variable (refer to the units table)

In QC, the value here is the high limit assay value.

1.4.4.1.1.14 Field 14 - RBC

Please refer to the WBC field description for details. The
field ID is RBC.
1.4.4.1.1.15 Field 15 - HGB
Please refer to the WBC field description for details. The
field ID is HGB.
1.4.4.1.1.16 Field 16 - HCT
Please refer to the WBC field description for details. The
field ID is HCT.

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 1.4.4.1.1.17 Field 17 - MCV
Please refer to the WBC field description for details. The
field ID is MCV.
1.4.4.1.1.18 Field 18 - MCH
Please refer to the WBC field description for details. The
field ID is MCH.
1.4.4.1.1.19 Field 19 - MCHC
Please refer to the WBC field description for details. The
field ID is MCHC.
1.4.4.1.1.20 Field 20 - RDW
Please refer to the WBC field description for details. The
field ID is RDW.
1.4.4.1.1.21 Field 21 - PLT
Please refer to the WBC field description for details. The
field ID is PLT.
1.4.4.1.1.22 Field 22 - MPV
Please refer to the WBC field description for details. The
field ID is MPV.
1.4.4.1.1.23 Field 23 - PCT*
Please refer to the WBC field description for details. The
field ID is PCT.
1.4.4.1.1.24 Field 24 - PDW*
Please refer to the WBC field description for details. The
field ID is PDW.
1.4.4.1.1.25 Field 25 - LYM%
Please refer to the WBC field description for details. The
field ID is LYM%.
1.4.4.1.1.26 Field 26 - MID%
Please refer to the WBC field description for details. The
field ID is MID%.
1.4.4.1.1.27 Field 27 - GRA%
Please refer to the WBC field description for details. The
field ID is GRA%.
1.4.4.1.1.28 Field 28 - LYM
Please refer to the WBC field description for details. The
field ID is LYM.
1.4.4.1.1.29 Field 29 - MID
Please refer to the WBC field description for details. The
field ID is MID.

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 1.4.4.1.1.30 Field 30 - GRA
Please refer to the WBC field description for details. The
field ID is GRA.
1.4.4.1.1.31 Field 31 - END RESULT
Please refer to Section 1.4.2.3.1.41

*Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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 1.4.4.1.2 QC Result example
RESULT[CR]
DATE;21/06/2008[CR]
TIME;10:08:25[CR]
MODE;QC[CR]
UNIT;1[CR]
SEQ;1;0[CR]
LOT;16961CD[CR]
LEVEL;L[CR]
LOT DATE;21/06/2008;10:07:59[CR]
EXPIRY DATE;07/06/2009[CR]
USER;OG[CR]
TEST;LMG[CR]
OPERATOR;OG[CR]
WBC;8.0 ;;L;15.0 ;;;20.0 [CR]
RBC;4.80 ;;;4.68 ;;;5.28 [CR]
HGB;11.5;;L;14.4;;;15.8[CR]
HCT;40.5;;;37.8;;;44.8[CR]
MCV;84.4 ;;;79.0 ;;;87.0 [CR]
MCH;24.0 ;;L;27.8 ;;;32.8 [CR]
MCHC;28.4 ;;L;33.2 ;;;39.8 [CR]
RDW;16.6 ;;;0.0 ;;;99.8 [CR]
PLT;220 ;;L;407 ;;;527 [CR]
MPV;15.8 ;;;0.0 ;;;99.8 [CR]
*PCT;0.580;;H;0.000;;;0.000[CR]
*PDW;12.2 ;;H;0.0 ;;;0.0 [CR]
LYM%;28.1;;L;46.8;;;56.8[CR]
MID%;29.5;;H;6.2 ;;;12.2[CR]
GRA%;42.4;;;34.0;;;44.0[CR]
LYM;2.2 ;;L;6.8 ;;;11.4 [CR]
MID;2.4 ;;;0.8 ;;;2.4 [CR]
GRA;3.4 ;;L;4.8 ;;;8.8 [CR]
END_RESULT;29885[CR] ...

* Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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 1.4.5 Precision

Emerald Host
Sending precision FRAME HEADER[CR]
result requested RESULT_READY;2560[CR]

Sending precision ACK_RESULT_READY[CR]

request acknowledge

Precision result sending FRAME HEADER[CR]

RESULT[CR]RESULT DATA SEGMENT
Precision result
ACK_RESULT;OK[CR]
acknowledge

1.4.5.1 Precision Result

The Precision result segment is a subset of the fields described in the
analysis result segment with some specific details listed below.
1.4.5.1.1 Precision result data segment
1.4.5.1.1.1 Field 1 - Date Field
The Date, the date on which the precision run was done,
is an alphanumeric field of ten (10) characters. The
default format of the date is DD/MM/YYYY.

Field ID Field ID length Data Format Length

DATE 4 DD/MM/YYYY 10

1.4.5.1.1.2 Field 2 - Time Field

The Time is an alphanumeric field of eight (8) characters.
It gives the time at which the precision run was done in
standard 24-hour format.

Field ID Field ID length Data Format Length

TIME 4 HH:MM:SS 8

1.4.5.1.1.3 Field 3 - Mode

The mode field is an alphanumeric field of up to thirteen
(13) characters. It gives the analysis mode: here
REPEATABILITY.

Field ID Field ID length Data Format Length

MODE 4 REPEATABILITY 13

1.4.5.1.1.4 Field 4 - Unit

Please refer to Section 1.4.2.3.1.4
1.4.5.1.1.5 Field 5 - Seq
Please refer to Section 1.4.2.3.1.5

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 1.4.5.1.1.6 Field 6 - Test
Please refer to Section 1.4.2.3.1.10
1.4.5.1.1.7 Field 7 - Operator
Please refer to Section 1.4.2.3.1.11
1.4.5.1.1.8 Field 8 - WBC
NOTE: To better understand Flagging fields, refer to Section 3 in
the CELL-DYN Emerald System Operator’s Manual.
•The count values transmitted to the host are sent in the
selected unit.
•For Rank 3: ‘*’ is used for L1 and/or L5 WBC flags and ‘s’
is used for L2 and/or L3 WBC flags. If both flags are
generated simultaneously, ‘*’ will supercede ‘s’.

rank Data values Data Format Length

1 Field ID WBC 3

2 Count value Numeric Variable (refer to the units table). Maximum 5

+++++ 5
----- 5

3 Suspicion flag Empty, ‘s’ or ‘*’ Up to 1 character

4 Flag: overange or Empty or ‘D’ or ‘L’ Up to 1 character

panic flags or ‘l’ or ‘h’ or ‘H’

5 Low Panic value Empty 0

6 Low value Empty 0

7 High value Empty 0

8 High panic value Empty 0

1.4.5.1.1.9 RBC- Field 9

Please refer to the WBC field description for details. The
field ID is RBC.
1.4.5.1.1.10 HGB- Field 10
Please refer to the WBC field description for details. The
field ID is HGB.
1.4.5.1.1.11 Field 11 - HCT
Please refer to the WBC field description for details. The
field ID is HCT.
1.4.5.1.1.12 Field 12 - MCV
Please refer to the WBC field description for details. The
field ID is MCV.
1.4.5.1.1.13 Field 13 - MCH
Please refer to the WBC field description for details. The
field ID is MCH.

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 1.4.5.1.1.14 Field 14 - MCHC
Please refer to the WBC field description for details. The
field ID is MCHC.
1.4.5.1.1.15 Field 15 - RDW
Please refer to the WBC field description for details. The
field ID is RDW.
1.4.5.1.1.16 Field 16 - PLT
Please refer to the WBC field description for details. The
field ID is PLT.
1.4.5.1.1.17 Field 17 - MPV
Please refer to the WBC field description for details. The
field ID is MPV.
1.4.5.1.1.18 Field 18 - PCT*
Please refer to the WBC field description for details. The
field ID is PCT.
1.4.5.1.1.19 Field 19 - PDW*
Please refer to the WBC field description for details. The
field is PDW.
1.4.5.1.1.20 Field 20 - LYM%
Please refer to the WBC field description for details. The
field is LYM%.
1.4.5.1.1.21 Field 21 - MID%
Please refer to the WBC field description for details. The
field is MID%.
1.4.5.1.1.22 Field 22 - GRA%
Please refer to the WBC field description for details. The
field is GRA%.
1.4.5.1.1.23 Field 23 - LYM
Please refer to the WBC field description for details. The
field is LYM.
1.4.5.1.1.24 Field 24 - MID
Please refer to the WBC field description for details. The
field is MID.
1.4.5.1.1.25 Field 25 - GRA
Please refer to the WBC field description for details. The
field is GRA.
1.4.5.1.1.26 Field 26 - END RESULT
Please refer to section 1.4.2.3.1.41

*Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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 1.4.5.1.2 Precision result example
RESULT[CR]
DATE;21/06/2008[CR]
TIME;10:23:09[CR]
MODE;REPEATABILITY[CR]
UNIT;1[CR]
SEQ;2;0[CR]
TEST;LMG[CR]
OPERATOR;OG[CR]
WBC;12.0 ;;;;;;[CR]
RBC;4.70 ;;;;;;[CR]
HGB;11.4;;;;;;[CR]
HCT;40.4;;;;;;[CR]
MCV;86.0 ;;;;;;[CR]
MCH;24.3 ;;;;;;[CR]
MCHC;28.2 ;;;;;;[CR]
RDW;16.2 ;;;;;;[CR]
PLT;220 ;;;;;;[CR]
MPV;10.4 ;;;;;;[CR]
*PCT;0.380;;;;;;[CR]
*PDW;12.0 ;;;;;;[CR]
LYM%;9.3 ;;;;;;[CR]
MID%;9.7 ;;;;;;[CR]
GRA%;81.0;;;;;;[CR]
LYM;1.1 ;;;;;;[CR]
MID;1.2 ;;;;;;[CR]
GRA;9.7 ;;;;;;[CR]
END_RESULT;59882[CR]
1.5 Control sum
The control sum is a CRC code: CRC-16.
1.5.1 Algorithm
The source code used is below(C code).
It allows generation of standard CRC-16.
This algorithm is mainly used in embedded systems (like a hard disk controller).
For best performance, computation is done from a seek table with 16 values.
Here is the CRC computation:
CRC = 0xFFFF
For each nibble:
Work on high weight nibble:
Index = byte EXCLUSIVE OR CRC
Index = Index AND 000F
CRC = Table (Index) EXCLUSIVE OR (CRC divided by 16)

Index = byte divided by 16

Index = Index EXCLUSIVE OR CRC
Index = Index AND 000F
CRC = Table (Index) EXCLUSIVE OR (CRC divided by 16)

* Clinical significance has not been established for these parameters; therefore they are not reportable in the U.S. These options
may only be selected outside of the U.S.

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9159926A—December 2020
 C language source example implementation:
Seek table declaration:
static const unsigned short ausCrcTab1[] =
{
0x0000, 0xCC01,0xD801,0x1400,0xF001,0x3C00,0x2800,0xE401,
0xA001,0x6C00,0x7800,0xB401,0x5000,0x9C01,0x8801,0x4400,
};

CRC computation:
unsigned short calc_crc(unsigned char *pucData, long lSize)
{
unsigned short usAcc1 = 0xFFFF;

while ( lSize > 0 )

{
usAcc1 = ausCrcTab1[(*pucData ^ usAcc1) & 15] ^ (usAcc1 >> 4);
usAcc1 = ausCrcTab1[((*pucData >> 4) ^ usAcc1) & 15] ^ (usAcc1 >>
4);

pucData++;
lSize--;
}

return(usAcc1);
}

^: EXCLUSIVE OR
>>: LOGICAL RIGHT SHIFT.
&: LOGICAL AND.
This CRC is computed from the beginning of the sent data until the end of the line ([CR]
included) preceding the checksum line (Identifier + value).

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 1.5.2 Example
The CRC is computed with all grey background data:
EMERALD;INSTRUMENT NUMBER;INSTRUMENT SERIAL NUMBER;USER LOGIN[CR]
RESULT[CR]
DATE;06/06/2008[CR]
TIME;13:41:29[CR]
MODE;NORMAL[CR]
UNIT;1[CR]
SEQ;31;0[CR]SID;No ID Entered[CR]
PID;[CR]
ID;[CR]
TYPE;STANDARD[CR]
TEST;LMG[CR]
OPERATOR;OG[CR]
WBC;12.0 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
RBC;5.20 ;;H;0.00 ;0.00 ;0.00 ;0.00 [CR]
HGB;11.9;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
HCT;40.9;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MCV;78.7 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MCH;22.9 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MCHC;29.1 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
RDW;17.7 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
PLT;220 ;;H;0 ;0 ;0 ;0 [CR]
MPV;7.6 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
LYM%;22.5;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MID%;23.7;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
GRA%;53.8;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
LYM;2.7 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
MID;2.8 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
GRA;6.5 ;;H;0.0 ;0.0 ;0.0 ;0.0 [CR]
WBC
CURVE;0;0;0;0;0;0;0;0;1;5;13;25;40;56;73;91;109;128;146;165;184;202;
220;235;244;245;240;229;215;199;182;164;145;127;108;89;71;54;40;31;25;
21;18;17;17;17;17;17;17;18;20;23;25;27;29;32;35;37;39;41;44;47;49;51;
53;56;59;61;63;65;68;71;73;75;77;79;82;84;86;88;91;94;96;98;98;98;97;
95;94;92;89;87;85;83;81;78;75;73;71;69;66;63;61;59;57;54;51;49;47;45;
42;39;37;35;33;30;27;23;17;11;6;2;0;0;0;0;0;0;[CR]
WBC THRESHOLDS;25;37;0;[CR]
RBC
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;1;3;6;
12;20;30;42;56;70;86;102;119;136;153;170;186;201;213;221;225;225;221;
214;204;192;178;163;147;130;113;96;80;64;50;37;27;19;13;8;5;3;1;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;[CR]

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 RBC THRESHOLDS;32;55
[CR]PLT
CURVE;0;0;0;0;0;0;1;2;4;7;10;14;19;23;29;34;40;46;52;58;64;70;77;83;
89;95;100;104;108;110;112;113;114;113;113;112;111;109;107;105;103;101;
98;96;94;91;89;86;83;81;78;75;73;70;68;65;63;60;57;55;52;49;47;44;41;
39;36;33;31;28;25;23;20;17;15;12;10;8;6;5;4;3;2;1;1;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
[CR]
PLT THRESHOLDS;100[CR]
ALARMS;QC FAIL;INS-T;[CR]
INTERPRETIVE_WBC;LEU>;LYM>;GRA>;[CR]
INTERPRETIVE_RBC;ERY>;MACRO;[CR]
INTERPRETIVE_PLT;THR>;GIANTP;[CR]
COMMENT;;[CR]

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CELL-DYN Emerald LIS Quick Reference Guide

Page 42 of 44 CELL-DYN Emerald LIS Interface Specification

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CELL-DYN Emerald LIS Interface Specification Page 43 of 44
9159926A—December 2020
 NOTES

Page 44 of 44 CELL-DYN Emerald LIS Interface Specification

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