1100 Series Thermos Tatted Column Compartment Reference Manual G1316-90004

Agilent 1100 Series Thermostatted Column Compartment
Reference Manual
Notices
Agilent Technologies, Inc. 1995, 1996-2004 No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.
Warranty
The material contained in this document is provided as is, and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.
rights not conveyed under these terms, it must negotiate with Agilent to establish acceptable terms in a written agreement executed by all relevant parties.
Safety Notices
Manual Part Number

G1316-90004
CAUTION
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.
Edition
Edition 05/2004 Printed in Germany Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn, Germany
Software Revision
This guide is valid for A.01.xx revisions of the Agilent 1100 Series Thermostatted Column Compartment software, where xx refers to minor revisions of the software that do not affect the technical accuracy of this guide.
Technology Licenses
The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.
WA R N I N G
A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.
Restricted Rights Legend

Software and technical data rights granted to federal government customers include only those rights customarily provided to end user Customers of Software. Agilent provides this customary commercial license in Software and technical data pursuant to FAR 12.211 (Technical Data) and FAR 12.212 (Computer Software) and, for Department of Defense purchases, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commercial Computer Software or Computer Software Documentation). If a federal government or other public sector Customer has a need for
1100 Series TCC Reference Manual
In This Guide
1 Installing the Column Compartment
How to install the thermostatted column compartment
How to optimize the Column Compartment

An introduction to the column compartments optimization
Troubleshooting and Test Functions

This chapter describes the instruments built in troubleshooting and test functions.
Repairing the Column Compartment

Instructions on how to repair the column compartment
Identifying Parts and Materials

Detailed illustrations and listings for parts and materials identification
Introduction to the Column Compartment

An introduction to the column compartment, instrument overview, theory of operation, external communication and internal connectors
Control Module Sceens for the Agilent 1100 Column Compartment

This chapter is intended to introduce an operator to the screens available for operation of the Agilent 1100 thermostatted column compartment (TCC) with the Agilent 1100 control module. Please use the manual of control module for further detailed reference.
Specifications
Performance specifications of the thermostatted column compartment
Contents
1 Installing the Column Compartment Site Requirements 12 14 15 17 20 23 27 11
Physical Specifications
Unpacking the Column Compartment Optimizing the Stack Configuration Installing the Column Compartment
Flow Connections of the Column Compartment 2 How to optimize the Column Compartment Optimizing the Column Compartment 3 Troubleshooting and Test Functions 28 29
Overview of the Column Departments Indicators and Test Functions Status indicators 31 Power Supply Indicator 32 Instrument Status Indicator 32 Error Messages 33 Timeout 34 Shutdown 35 Remote Timeout 36 Synchronization Lost 37 Leak 38 Leak Sensor Open 39 Leak Sensor Short 40 Compensation Sensor Open
30
41
Contents
Compensation Sensor Short 42 Left Fan Failed 43 Right Fan Failed 44 Open Cover 45 Cover Violation 46 Left Temperature Timeout 47 Right Temperature Timeout 48 Defective Temperature Sensor 49 Heater Profile 50 Valve Failed 51 Column Temperature 52 Heatsink Temperature 53 Defective Heater Circuit 54 Thermostat Function Test 55
Temperature Calibration 57 Temperature Calibration Procedure 58 Calibration Problems 59 Installing the Temperature Sensor 60 4 Repairing the Column Compartment 63 64 67
Introduction into Repairing the Column Compartment Using the ESD Strap 66 Overview of the Repairing the Column Compartment Simple Repairs 68 Changing Column Identification Tags 69 Replacing Head Parts of Column Switching Valve Correcting Leaks 74 Exchanging Internal Parts 75 Removing the Top Cover and Foam 77 Removing the Column Switching Valve 80 Installing the Column Switching Valve 83
72
Contents
Exchanging the Column Compartment Main (CCM) Board 86 Replacing the Column Identification Module (CID) Board 90 Exchanging the Fan 91 Removing the Heat Exchanger Assemblies 93 Installing the Heat Exchanger Assemblies 96 Exchanging the Power Supply 99 Replacing the Leak Sensor or Leak Base 102 Replacing Status Light Pipe 107 Installing the Foam and the Top Cover 108 Assembling the Main Cover 111 Replacing the Column Compartments Firmware 112 5 Identifying Parts and Materials Overview of Main Assemblies Column Switching Valve 2 position / 10 port Valve Control Module Sheet Metal Kit Plastic Parts Foam Parts 119 120 121 122 123 116 118 113 114
Power and Status Light Pipes Leak Parts Accessory Kit 124 125
Cable Overview 126 Analog Cables 128 Remote Cables 131 BCD Cables 138 Auxiliary Cable 140 CAN Cable 141
Contents
External Contact Cable RS-232C Cable Kit LAN Cables 6 144 143
142
Introduction to the Column Compartment Introduction to the Column Compartment System Overview 147 149 150 153
145 146
Column-Identification System Column-Identification Tag Column Clip 152
Column Switching Valve (Optional) Electrical Connections Instrument Layout The Electronics 157 158 155
Column Compartment Main Board (CCM) Firmware Description Firmware Updates 164 165 166
159
Agilent 1100 Series Interfaces GPIB Interface 167 CAN Interface 167 Remote Interface 167 RS-232C 169
Setting the 8-bit Configuration Switch 171 GPIB Default Addresses 172 Communication Settings for RS-232C Communication Forced Cold-Start Settings 174 Stay Resident Settings 175 The Main Power Supply Assembly 176
173
Contents
Control Module Screens for the Agilent 1100 Column Compartment Major keys on the Agilent 1100 Control Module Screens available from the Analysis screen Screens available from the System screen Screens available from the Records screen Diagnostics and Tests 201 181 192 194 180
179
Specifications
203 204
Performance Specifications A Safety Informations 207
General Safety Information Lithium Batteries Information Radio Interference Sound Emission 212 213 214
208 211
Solvent Information
Agilent Technologies on Internet Index 217
216
Contents
10
Agilent 1100 Series Thermostatted Column Compartment Reference Manual
1 Installing the Column Compartment

Site Requirements 12 Physical Specifications 14 Unpacking the Column Compartment 15 Optimizing the Stack Configuration 17 Installing the Column Compartment 20 Flow Connections of the Column Compartment 23
Agilent Technologies
11
Installing the Column Compartment
Site Requirements
A suitable environment is important to ensure optimum performance of the column compartment.
Power Consideration
The column compartment power supply has wide-ranging capability (see Table 1 on page 14). It accepts any line voltage in the above mentioned range. Consequently there is no voltage selector in the rear of the column compartment. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.
WA R N I N G
To disconnect the column compartment from line, unplug the power cord. The power supply still uses some power, even if the power switch on the front panel is turned off.
WA R N I N G
Shock hazard or damage of your instrumentation can result, if the devices are connected to a line voltage higher than specified.
CAUTION
Make sure to have easy access to the power cable of the instrument, in order to disconnect the instrument from line.
Power Cords
Different power cords are offered as options with the column compartment. The female end of each of the power cords is identical. It plugs into the power-input socket at the rear of the column compartment. The male end of each of the power cords is different and designed to match the wall socket of a particular country or region.
12
WA R N I N G
Never operate your instrumentation from a power outlet that has no ground connection. Never use a power cord other than the power cord designed for your region.
WA R N I N G
Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Bench Space
The column compartment dimensions and weight (see Table 1 on page 14) allow to place the column compartment on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for the circulation of air and electric connections. If the bench should carry a complete Agilent 1100 Series system, make sure that the bench is designed to carry the weight of all the modules. The detector should be operated in a horizontal position.
Environment
Your column compartment will work within specifications at ambient temperatures and relative humidity as described in Table 1 on page 14.
CAUTION
Do not store, ship or use your column compartment under conditions where temperature fluctuations could cause condensation within the column compartment. Condensation will damage the system electronics. If your column compartment was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation.
13
Table 1
Type Weight
Specification 10.2 kg (22.5 lbs) 410 435 140 mm (16.1 17 5.5 inches) 100 120 or 220 240 VAC, 50 or 60 Hz, 5 % 320 VA / 150 W / 512 BTU 0 55 C (32 131 F) -40 70 C (-4 158 F) <95 %, at 25 40 C (77 104 F) Up to 2000 m (6500 ft.) Up to 4600 m (14950 ft.) Installation Category II, Pollution Degree 2 For storing the instrument Non-condensing Maximum Comments
Dimensions (width depth height) Line Voltage Line frequency Power consumption Ambient operating temperature Ambient non-operating temperature Humidity Operating altitude Non-operating altitude Safety standards: IEC, CSA, UL, EN
10 %
Wide-ranging capability
14
Unpacking the Column Compartment
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the column compartment may have been damaged during shipment.
CAUTION
If there are signs of damage, please do not attempt to install the column compartment.
Delivery Checklist
Ensure all parts and materials have been delivered with the column compartment. The delivery checklist is shown in Table 2. Please report missing or damaged parts to your local Agilent Technologies sales and service office. Table 2
Description Thermostatted column compartment Power cable CAN cable (Part number 5181-1516) Column switching valve Reference Manual Accessory kit (see Table 3 on page 16)
Column Compartment Delivery Checklist

Quantity 1 1 1 optional 1 1
15
Accessory Kit Contents

Table 3
Description Tubing flexible (to waste) for re-order 5 m Capillary, 90 mm lg, 0.17 i.d., fittings need to be assembled Ferrule front SST Ferrule back SST Fitting SST Column-identification tag Column clip ESD Wrist Strap
Accessory Kit Contents (Part number G1316-68705)

Part Number 5062-2463 G1316-87300 0100-0043 0100-0044 79814-22406
* **
Quantity 1.2 m 1 2 2 2 1 2 1
9300-1408
* for reordering use part number 5062-8588 that comprises a kit with 3 column identification tags ** for reordering use part number 5063-6526 that comprises a kit with 6 column clips
Ferrule front Ferrule back Fitting Capillary
Figure 1
Capillary (Column-Heat Exchanger) Parts
16
Optimizing the Stack Configuration
If your column compartment is part of a Agilent 1100 Series system, you can ensure optimum performance by installing the following configuration. This configuration optimizes the system flow path and ensures minimum delay volume
17
Solvent cabinet
Vacuum degasser
Pump
Autosampler Control module
Column compartment
Detector
Figure 2
Recommended Stack Configuration (Front View)
18
Remote cable Analog pressure signal
CAN Bus cable
CAN Bus cable
AC power Analog signal to recorder GPIB or LAN to LC ChemStation
Figure 3
Recommended Stack Configuration (Rear View)
19
Preparations
Locate bench space. Provide power connections. Unpack the Column compartment. Column compartment Power cord, for other cables see text below and Column Switching Valve on page 116.
Parts required
1 Place the column compartment in the stack or on the bench in a horizontal position. 2 Ensure the power switch at the front of the column compartment is OFF.
Status indicator green/yellow/red
Line power switch with green light
Figure 4
Front View of the Thermostatted Column Compartment
3 Connect the power cable to the power connector at the rear of the column compartment. 4 Connect the CAN cable to other Agilent 1100 Series modules.
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5 If a Agilent ChemStation is the controller, connect either the GPIB cable to the detector or the LAN connection to the LAN interface board in the detector.
NOTE
If a Agilent 1100 DAD/MWD/FLD is in the system, the LAN/GPIB should be connected to the DAD/MWD/FLD (due to higher data load).
6 Connect the APG Remote cable (optional) for non-Agilent 1100 Series instruments. 7 Turn on power by pushing the button at the lower left side of the column compartment. The status LED should shine green.
Security lever
APG Remote RS-232C
CAN
GPIB
Power
Configuration switch
Figure 5
Rear View of the Thermostatted Column Compartment
NOTE
The column compartment is turned on when the line power switch is pressed and the green indicator lamp is illuminated. The column compartment is turned off when the line power switch is protruding and the green light is off.
21
WA R N I N G
To disconnect the column compartment from line, unplug the power cord. The power supply still uses some power, even if the power switch at the front panel is turned off.
NOTE
The column compartment was shipped with default configuration settings. For changing of these setting, see Setting the 8-bit Configuration Switch on page 171.
22
Flow Connections of the Column Compartment
WA R N I N G
When working with solvents please observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used.
Preparations Parts required
Install the column compartment Other modules Parts from accessory kit, see Accessory Kit Contents on page 16 and/or from capillary kit column switching valve, see Column Switching Valve on page 116 Two wrenches 1/4 5/16 inch for capillary connections
1 Press release buttons and remove front cover to gain access to heater area.
2 The column compartment is equipped with an column-identification system that can read column tags. Antennas
Column tag
Column clip
23
For more information on column identification, see Column-Identification System on page 149.
The internal volumes of the heat exchanger assemblies comprise a volume of 3 l (left) and 6 l (right). The internal capillary diameter is 0.17 mm.
3 Place the column on the left heat exchanger assembly and connect the capillaries to the column. From autosampler
4 Or place the column on the right heat exchanger assembly and connect the capillaries to the column.
To detector
To connect the column selection valve, see Column Switching Valve (Optional) on page 153.
24
5 Fix the column with the column clip from the accessory kit. From autosampler
If your accessory kit was shipped without the column clip, you may order them. Refer to Accessory Kit Contents on page 16 for part number information.
To detector
6 If the column compartment is not part of a Agilent 1100 Series system, or if an Agilent 1100 Series Autosampler is located on top, connect the corrugated tubing to the waste outlet.
7 Route tubings from modules above through the openings in the funnel holder (top) and the plastic bottom part. Remove small plastic plugs first.
25
8 Close the front cover and replace the front cover.
The installation of the column compartment has now been completed.
NOTE
The moduler should be operated with the front cover in place for proper thermostatting condiotion and to protect thecolumn areaarea against strong drafts from the ouside.
26
2 How to optimize the Column Compartment

Optimizing the Column Compartment 28
27
How to optimize the Column Compartment
Optimizing the Column Compartment
For best performance results of the column compartment follow the following hints: Use short connection capillaries and place them close to the heat exchanger. This will reduce heat dissipation and external band-broadening. Use the left heat exchanger for small volume columns, for example, 23 mm i.d. columns at flow rates of less than 200 l/min. For even lower band-broadening, the heat exchanger can be by-passed and the column is placed well between the heat exchanger fins. Keep the left and right heat exchanger temperature the same unless you do specific applications. Assure that the front cover is always closed.
28
3 Troubleshooting and Test Functions

Overview of the Column Departments Indicators and Test Functions 30 Status indicators 31 Error Messages 33 Thermostat Function Test 55 Temperature Calibration 57
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Overview of the Column Departments Indicators and Test Functions
Status Indicators
The instrument is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the instrument. The status indicators provide a quick visual check of the operation of the instrument.
Error Messages
In the event of an electronic, mechanical or hydraulic failure, the instrument generates an error message in the user interface. The following pages describe the meaning of the error messages. For each message, a short description of the failure, a list of probable causes of the problem, and a list of suggested actions to fix the problem are provided.
Thermostat Function Test

The thermostat function test evaluates the heating and cooling efficiency of the two peltier elements.
Temperature Calibration and Verification

The temperature calibration and verification procedure enables the instrument temperature to be measured against an external, calibrated measuring device. Normally, temperature calibration is not required throughout the lifetime of the instrument. However, in order to comply with local regulatory requirements, calibration and verification may be required. The following sections describe these functions in detail.
30
Status indicators
Two status indicators are located on the front of the instrument. The lower left indicates the power supply status, the upper right indicates the instrument status.
Status indicator green/yellow/red
line power switch with green light
Figure 6
Location of Status indicators
31
Power Supply Indicator

The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON.
Instrument Status Indicator

The instrument status indicator indicates one of four possible instrument conditions: When the status indicator is OFF (and power switch indicator is on), the instrument is in a prerun condition, and is ready to begin an analysis. A green status indicator, indicates the instrument is performing an analysis (run mode). A yellow indicator indicates a not-ready condition. The instrument is in a not-ready state when it is waiting for a specific condition or action to be completed (for example, immediately after changing a setpoint), or while a self-test procedure is running. An error condition is indicated when the status indicator is red. An error condition indicates the instrument has detected an internal problem which affects correct operation of the instrument. Usually, an error condition requires attention (for example, leak, defective internal components). An error condition always interrupts the analysis.
32
Error Messages
Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, frit exchange, exchange of consumable is necessary). In the event of such a failure, the red status indicator at the front of the column compartment is switched on, and an entry is written into the instrument logbook. This section describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
33
Timeout
The timeout threshold was exceeded.
Probable Causes
The analysis was completed successfully, and the timeout function switched off the pump as requested. A not-ready condition was present during a sequence or multiple-injection run for a period longer than the timeout threshold.
Suggested Actions
Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required.
34
Shutdown
An external instrument has generated a shut-down signal on the remote line. The instrument continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Probable Causes
Leak detected in an external instrument with a remote connection to the system. Shut-down in an external instrument with a remote connection to the system. The degasser failed to generate sufficient vacuum for solvent degassing.
Suggested Actions
Fix the leak in the external instrument before restarting the pump module. Check external instruments for a shut-down condition. Check the degasser module for an error condition. Refer to the Reference Manual for the Agilent 1100 Series vacuum degasser.
35
Remote Timeout
A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (e.g. a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis. If a not-ready condition is still present on the remote line after one minute the error message is generated.
Probable Causes
Not-ready condition in one of the instruments connected to the remote line. Defective remote cable. Defective components in the instrument showing the not-ready condition.
Suggested Actions
Ensure the instrument showing the not-ready condition is installed correctly, and is set up correctly for analysis. Exchange the remote cable. Check the instrument for defects (refer to the instruments reference documentation).
36
Synchronization Lost
During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.
Probable Causes
CAN cable disconnected. Defective CAN cable. Defective main board in another module.
Suggested Actions
Ensure all the CAN cables are connected correctly. Switch off the system. Restart the system, and determine which module or modules are not recognized by the system. Ensure all CAN cables are installed correctly.
37
Leak
A leak was detected in the column compartment module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent. This changes the resistance of the leak sensor which is sensed by the leak-sensor circuit on the CCM board.
Probable Causes
Condensation. Loose column fittings. Broken capillary. Leaking column-switching valve seal.
Suggested Actions
Use a higher temperature setpoint. Ensure all fittings are tight. Exchange defective capillaries. Exchange the valve seal.
38
Leak Sensor Open

The leak sensor in the column compartment module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits. If the current falls outside the lower limit, the error message is generated.
Probable Causes
Leak sensor not connected to the CCM board. Defective leak sensor.
Suggested Actions
Ensure the leak sensor is connected correctly. Exchange the leak sensor.
39
Leak Sensor Short

The leak sensor in the column compartment module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits. If the current increases above the upper limit, the error message is generated.
Probable Causes
Defective leak sensor.
Suggested Actions
Exchange the leak sensor.
40
Compensation Sensor Open

The ambient-compensation sensor (NTC) on the CCM board in the column compartment module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the CCM board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor increases above the upper limit, the error message is generated.
Probable Causes
Defective CCM board.
Suggested Actions
Exchange the CCM board.
41
Compensation Sensor Short

The ambient-compensation sensor (NTC) on the CCM board in the column compartment module has failed (short circuit). The resistance across the temperature compensation sensor (NTC) on the CCM board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor falls below the lower limit, the error message is generated.
Probable Causes
Suggested Actions
42
Left Fan Failed

The left cooling fan in the column compartment has failed. The hall sensor on the fan shaft is used by the CCM board to monitor the fan speed. If the fan speed falls below 2 revolutions/second for longer than 5 seconds, the error message is generated.
Probable Causes
Fan cable disconnected. Defective fan. Defective CCM board.
Suggested Actions
Ensure the fan is connected correctly. Exchange fan. Exchange the CCM board.
43
Right Fan Failed

The right cooling fan in the column compartment has failed. The hall sensor on the fan shaft is used by the CCM board to monitor the fan speed. If the fan speed falls below 2 revolutions/second for longer than 5 seconds, the error message is generated.
Probable Causes
Fan cable disconnected. Defective fan. Defective CCM board.
Suggested Actions
Ensure the fan is connected correctly. Exchange the fan. Exchange the CCM board.
44
Open Cover
The top foam has been removed. The sensor on the CCM board detects when the top foam is in place. If the foam is removed, the fan is switched and peltier elements are switched off, and the error message is generated.
Probable Causes
The top foam was removed during operation. Foam not activating the sensor.
Suggested Actions
Replace the top foam. Exchange the foam.
45
Cover Violation
The column compartment was switched on with the top cover and foam open. The sensor on the CCM board detects when the top foam is in place. If the column compartment is switched on with the foam removed, the processor switches off the peltier elements after a short delay, and the error message is generated.
Probable Causes
The column compartment was switched on with the top cover and foam removed.
Suggested Actions
Replace the top cover and foam.
46
Left Temperature Timeout

The temperature of the left heat exchanger did not reach the temperature setpoint within the timeout threshold.
Probable Causes
Timeout threshold too short. Defective left heater assembly. Defective CCM board.
Suggested Actions
Increase the timeout threshold value. Exchange the heater assembly. Exchange the CCM board.
47
Right Temperature Timeout

The temperature of the right heat exchanger did not reach the temperature setpoint within the timeout threshold.
Probable Causes
Timeout threshold too short. Defective right heater assembly. Defective CCM board.
Suggested Actions
Increase the timeout threshold value. Exchange the heater assembly. Exchange the CCM board.
48
Defective Temperature Sensor

Defective Temperature Sensor 0:left column. Defective Temperature Sensor 1:left heatsink. Defective Temperature Sensor 2:right column. Defective Temperature Sensor 3:right heatsink. Defective Temperature Sensor 4:ambient-correction sensor (located on left flex board). One of the temperature sensors has failed. The CCM board monitors the signal from the sensor continually. If the signal is missing or out of range, the error message is generated.
Probable Causes
Flex board not connected (only if all left or right sensor error messages appear simultaneously). Defective heater assembly. Defective CCM board.
Suggested Actions
Ensure the flex board is connected correctly. Exchange the heater assembly. Exchange the CCM board.
49
Heater Profile
Heater Profile 0: left heater. Heater Profile 2: right heater. The temperature warm-up (or cooling) profile of the heater is incorrect. When the temperature setpoint is changed, the heater begins heating (or cooling) the column heat exchanger. During this time, the processor monitors the temperature change, and checks if the temperature profile is changing in the correct direction. If the temperature is not changing as expected, the error message is generated.
Probable Causes
Defective heater assembly. Defective CCM board.
Suggested Actions
Exchange the heater assembly. Exchange the CCM board.
50
Valve Failed
Valve Failed 0:failed to switch to the position where ports 1 and 2 are connected. Valve Failed 1:failed to switch to the position where ports 1 and 6 are connected. The column-switching valve failed to switch. The switching of the column-switching valve is monitored by two micro switches on the valve assembly. The switches detect the successful completion of the valve movement within a predefined time window. If the valve fails to reach the end point, or fails to reach the end point within the time window, the error message is generated.
Probable Causes
Defective column-switching valve. Defective CCM board.
Suggested Actions
Exchange the column-switching valve. Exchange the CCM board.
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Column Temperature
Column Temperature 0: left heater. Column Temperature 2: right heater. The temperature of the column heat exchanger has exceed the maximum limit. For safety reasons, the maximum column heat-exchanger temperature is 105 C. If an electronic failure occurs which causes the heater to heat continually, the current is switched off when the temperature exceeds 105 C, and the error message is generated.
Probable Causes
Suggested Actions
52
Heatsink Temperature
Heatsink Temperature 0: left heater. Heatsink Temperature 2: right heater The temperature of the Peltier heatsink has exceed the maximum limit. The maximum temperature of the Peltier heatsink is 70 C. If an electronic failure occurs which causes the heatsink to reach 70 C, the current is switched off and the error message is generated.
Probable Causes
Suggested Actions
53
Defective Heater Circuit

The electronic circuit for control of the heater assemblies is defective. The processor checks the function of the heater circuits continually. If a defect is detected in the control circuit, the processor switches off the heater (peltier) assemblies, and the error message is generated.
Probable Causes
Suggested Actions
54
Thermostat Function Test
The thermostat function test is used to evaluate the cooling and heating performance of the two peltier elements.
Description
When the test is started, both heat exchangers are cooled initially to 25 C. This temperature is held for 12 seconds, and then the setpoint is changed to 20 C. The time required to reach 20 C is a measure of the cooling efficiency of the peltier elements. At 3.5 minutes, the setpoint is changed to 30 C, and both elements begin heating. The time required to reach 30 C is a measure of heating efficiency. A typical thermostat function test profile is shown in Figure 7.
Temperature [C]
Right Peltier element Left Peltier element
Time [minutes]
Figure 7
Typical Thermostat Function Test Profile
55
Evaluating the Thermostat Function Test

During the cooling phase, the Peltier elements should cool at a rate of >2 C minute. During the heating phase, the temperature change should be >3 C/minute. Defective thermostat components may cause cooling or heating rates to fall outside these limits.
Function Test Failed Probable Causes

Column compartment cover not installed correctly (bad insulation). Air intake blocked (insufficient air flow for cooling). Poor peltier efficiency (if setpoint temperatures can still be reached, and are stable, there is no requirement to exchange the heater assembly). Defective sensors on flex board. Defective heater assembly.
Suggested Actions
Ensure cover is installed correctly. Ensure sufficient space is available for air circulation (Bench Space on page 13). Exchange the heater assembly.
56
Temperature Calibration
Temperature Calibration Principle

The actual temperatures of the column heat exchangers (left and right) are dependent on the column setpoint temperature. For setpoint temperatures above 36 C, the heat exchangers are heated to a temperature slightly above the setpoint temperature. Conversely, for setpoint temperatures below 36 C, the heat exchangers are maintained at a temperature slightly below the setpoint temperature. This fine temperature correction compensates for the small amount of heat exchange through the instrument housing, and ensures the column is always maintained at the setpoint temperature. At 36 C, the column setpoint and heat-exchanger temperatures are equal (temperature cross-over point). This is the temperature at which a calibrated measuring device can be used to calibrate the column thermostat
Temperature difference [C] +1 temperature at measuring point 0 Column setpoint temperature
Calibration done at cross-over point (36C) -1 0 Temperature [C] 80
Figure 8
1-Point Calibration at the Temperature Cross-Over Point.
The column thermostat is calibrated correctly when the measured temperature (using the external measuring device, Temperature Calibration Procedure on page 58) and the cross-over temperature (36 C) of both heat exchangers (left and right) are within 0.5 C. 1100 Series TCC Reference Manual 57
Temperature Calibration Procedure

Tools required Parts required Temperature measuring device (see note below) Calibrated temperature measuring device.
NOTE
For the measuring and calibration process Agilent Technologies recommends a measuring device that provides the necessary resolution and precision. Contact the local Agilent Technologies support representative for ordering information.
NOTE
The figures below refer to a specific type of temperature sensor (Heraeus, Quat340, quartz surface-temperature measurement sensor). Other sensors may require a different fixing.
1 Install the temperature sensor (Installing the Temperature Sensor on page 60). 2 Select the column-compartment temperature calibration mode in the user interface. 3 Wait for the temperature to stabilize at the calibration temperature (36 C). 4 Measure the temperature of the heat exchanger. 5 If the measured temperature deviates by more than 0.5 C from the actual temperature, enter the measured value in the measured-temperature field for the left heat exchanger. 6 Install the sensor at the measurement point on the right heat exchanger. Repeat the calibration procedure for the right heat exchanger.
Limits
After calibration, the measured temperature and the calibration temperature should be within 0.5 C. The maximum deviation which can be adjusted is 1.8 C. If the measured value and the calibration value differ by more than 8 C, this is an indication that a problem exists, Calibration Problems on page 59.
58
Calibration Problems
If the temperature cannot be calibrated, check the following: Thermostat front cover is closed correctly. The measuring device is functioning correctly, and is calibrated according to the manufacturers instructions.
Hardware Failures
Probable hardware failures leading to a failed calibration procedure are: Defective or wrongly calibrated measuring device. Defective heater assembly. Defective ambient-temperature sensor. Defective CCM board.
59
Installing the Temperature Sensor

Installation of the temperature sensor is required for the temperature calibration and temperature verification procedures.
NOTE
The figures below refer to a specific type of temperature sensor (Heraeus, Quat340, quartz surface-temperature measurement sensor). Other sensors may require a different fixing.
1 Attach the thermal pad from the Calibration Kit onto the temperature sensor.
2 Insert the temperature sensor into the spring. Ensure the face of the thermal pad faces downwards.
Thermal pad
Thermal pad face
60
3 Remove the front cover.
4 Install the temperature sensor at the measurement position on the left heat exchanger.
5 Route the sensor wire through the slit in the leak tray.
6 Replace the front cover.
61
62
4 Repairing the Column Compartment

Introduction into Repairing the Column Compartment 64 Overview of the Repairing the Column Compartment 67 Simple Repairs 68 Exchanging Internal Parts 75
63
Introduction into Repairing the Column Compartment
Simple Repairs
The column compartment is designed for easy repair. The most frequent repairs such as change of column and column switching valve head parts can be done from the front of the column compartment with the column compartment in place in the system stack. These repairs are described in Simple Repairs on page 68.
Exchanging Internal Parts

Some repairs may require exchange of defective internal parts. Exchange of these parts requires removing the column compartment from the stack, removing the covers, and disassembling the column compartment. The security lever at the power input socket prevents that the column compartment cover is taken off when line power is still connected. These repairs are described in Exchanging Internal Parts on page 75.
WA R N I N G
To prevent personal injury, the power cable must be removed from the column compartment before opening the column compartment cover. Do not connect the power cable to the column compartment while the covers are removed.
WA R N I N G
To prevent personal injury, be careful when getting in contact with sharp metal areas.
WA R N I N G
64
CAUTION
Electronic boards and components are sensitive to electronic discharge (ESD). In order to prevent damage always use an ESD protection (for example, the ESD wrist strap from the accessory kit) when handling electronic boards and components, see Using the ESD Strap on page 66.
CAUTION
The column compartment has two heat exchanger assemblies that might be hot. If so, allow them to cool down before starting repairs.
Cleaning the Column Compartment

The column compartment case should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent. Do not use an excessively damp cloth that liquid can drip into the column compartment.
WA R N I N G
Do not let liquid drip into the column compartment. It could cause shock hazard and it could damage the column compartment.
65
Using the ESD Strap

Electronic boards are sensitive to electronic discharge (ESD). In order to prevent damage, always use an ESD strap supplied in the standard accessory kit (see Accessory Kit on page 125) when handling electronic boards and components. 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist. 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end. 3 Attach the copper foil to a convenient and exposed electrical ground.
Figure 9
Using the ESD Strap
66
Overview of the Repairing the Column Compartment
Figure 10 shows the main assemblies and their locations which can be repaired.
CID board, see page 90 Power supply, see page 99 CCM board, see page 86
Fan, see page 91
Heat exchanger Column switching valve, see page 83 and page 80
Fan
Leak sensor, see page 102 Heat exchanger, see page 93
Waste handling parts
Figure 10
Overview on Repair Procedures
NOTE
The shown CID board has been removed with TCC main board revision G1316-66530 in 2003. Its function has been incorporated into the main board.
67
Simple Repairs
The following sections describe repairs that can be done without opening the main cover. Table 4
Procedure Changing Column Identification Tags on page 69 Replacing Head Parts of Column Switching Valve on page 72 Correcting Leaks on page 74
Simple Repairs
Typical Frequency When column performance or new application requires a change When the valve performance shows indication of leakage or wear If leak has occurred Check for leaks Notes
68
Changing Column Identification Tags

Frequency Parts required If column is used on the opposite heat exchanger or a tag is added to a new column. Column identification tag, pack of 3, 5062-8588
The column compartment is equipped with an column-identification system, that stores column specific information. Two identification antennas are incorporated in the heat exchanger assemblies.
69
Antennas
Column-identification tag Column clip
Figure 11
Location of Column Identification System
When correctly placed on the heat exchanger, the distance between tag and antenna is 12 mm. This is the optimum distance for proper function. The identification tag can be easily removed from the column.
NOTE
For small diameter columns, a cable tie wrap should be used, to fix the column identification tag at the column. Assure that the tie wrap is not blocking the front cover.
70
NOTE
There is a difference in attaching the identification tag to the column depending on which heat exchanger it will be located, see Figure 12 and Figure 13. The Agilent logo should be always at the front side.
Column-identification tag
Figure 12
Column-Identification Tag for Left Heat Exchanger
Figure 13
Column-Identification Tag for Right Heat Exchanger
71
Replacing Head Parts of Column Switching Valve

Frequency Tools required Parts required If valve leaks 1/4 inch wrench 9/64 inch hex key Stator screws 1535-4857, stator head 0100-1850, stator face assembly 0100-1851, rotor seal 3 grooves (tefzel) 0100-1854, rotor seal 3 grooves (Vespel) 0100-1855, isolation seal 0100-1852, stator screws 1535-4857
Isolation seal Rotor seal Stator ring Stator face Stator head Stator screws Spring shows to rear
Figure 14
Column Switching Valve Parts
72
1 Remove capillaries from ports 1, 5, and 6.
2 Loosen each fixing stator screws two turns at a time. Remove bolts from head.
4 Remove the stator head and the ceramic stator face. 5 Remove the stator ring. 6 Remove the rotor seal (and isolation seal if damaged or contaminated). 7 Install the new isolation seal (if required). Ensure the metal spring inside the ring faces towards the valve body. 8 Install the new rotor seal. 9 Replace the stator ring. Ensure the stator ring is flush against the valve body. 10 Place the new (if required) ceramic stator face in place on the stator head. Reinstall the stator head. 11 Insert the stator screws in the stator head. Tighten the screws alternately two turns at a time until the stator head is secure. 12 Reconnect the pump capillaries to the valve ports. Slide the waste tube into the waste holder in the leak tray. 13 Perform a pressure-tightness test to ensure the valve is pressure tight to 400 bar.
73
Correcting Leaks
When required Tools required If a leakage has occurred at the heat exchanger or at the capillary connections or at the column switching valve Tissue Wrench 1/4 5/16 inch for capillary connections
1 Remove the front cover. 2 Use tissue to dry the leak sensor area. 3 Observe the capillary connections and the column switching valve for leaks and correct, if required. 4 Replace the front cover.
Column switching valve Column
Leak sensor assembly Waste outlet
Figure 15
Possible Leak Areas
74
Exchanging Internal Parts
WA R N I N G
The following procedures require opening the main cover of the column compartment. Always ensure the column compartment is disconnected from the line power when the main cover is removed. The security lever at the power input socket prevents that the column compartment cover is taken off when line power is still connected.
WA R N I N G
To disconnect the column compartment from line, unplug the power cord. The power supply still uses some power, even if the switch on the front panel is turned off.
WA R N I N G
To prevent personal injury, be careful when getting in contact with sharp metal areas.
WA R N I N G
NOTE
The electronics of the column compartment will not allow operation of the module when the top cover and the top foam are removed. A safety light switch on the main board will inhibit the operation of the fans immediately. Voltages for the other electronic components will be turned off after 30 seconds. The status lamp will light red and an error will be logged into the logbook of the user interface. Always operate the column compartment with the top covers in place.
75
CAUTION
Electronic boards and components are sensitive to electronic discharge (ESD). In order to prevent damage always use an ESD protection (for example, the ESD wrist strap from the start up kit) when handling electronic boards and components, see Using the ESD Strap on page 66.
CAUTION
The column compartment has two heater assemblies that might be hot. If so, allow them to cool down before starting repairs.
76
Removing the Top Cover and Foam

When required Tools required Parts required For all repairs inside the column compartment Screwdriver Pozidriv 1 PT3 Depends on the work inside and the following procedures
Preparations for this procedure: Turn off the column compartment. Disconnect the power cable. Disconnect capillaries. Remove column compartment from stack and place it on the working bench.
1 Press the release buttons and remove the front cover.
77
2 Pull the leak funnel out of the leak funnel holder.
3 Move the power lock across the power inlet and lift the clips on the rear of the cover.
4 Lift the cover up and slide it towards the rear.
5 Unscrew the screws at the rear of the top plate, slide the plate towards the front and remove it.
78
6 If installed. disconnect the connector of the column switching valve from column compartment and remove the top foam.
Do not connect a power plug to the column compartment after removing the top covers. A safety light switch on the main board will turn off fan (immediately) and electronics (after 30 seconds) to avoid the operation with removed covers. An error will be generated (status lamp lights red) and the logbook will show an error message. The next figures show the position of the light switch on the board.
Connector
7 Position of the foam in the safety light switch.
8 Position of safety light switch on the main board when removing the foam.
79
Removing the Column Switching Valve

When required Tools required If valve failed or bottom foam part has to be removed for other replacements Screwdriver Pozidriv 1 PT3 Wrench 1/4 5/16 inch for capillary connections
Preparations for this procedure: Turn off the column compartment. Disconnect the power cable. Disconnect capillaries. Remove column compartment from stack and place it on the working bench. Remove the front cover, top cover and top foam section, see Removing the Top Cover and Foam on page 77.
1 Disconnect the grounding connection of the valve at the Z-panel.
80
2 Unscrew the Z-panel.
3 Press against the rear of the Z-panel to release the metal plate from the guide and pull it carefully upwards.
4 Lift the Z-panel together with the top plastic panel half-way out of the guide.
5 Locate the ambient temperature sensor in the top plastic part and push it towards the rear.
81
6 Carefully remove the ambient temperature sensor plugged into the rear of the top plastic panel.
7 Pull the top plastic panel together with the Z-panel completely out of the guide.
8 Remove the Valve from its location.
For the installation refer to Installing the Column Switching Valve on page 83.
82
Installing the Column Switching Valve

When required Tools required For first time installation or after it was removed Screwdriver Pozidriv 1 PT3 Wrench 1/4 5/16 inch for capillary connections
Preparations for this procedure are: The column compartment is open as described in Removing the Column Switching Valve on page 80.
1 If no column switching valve was installed, remove the RFI-shield and the plastic cover (no longer used).
83
2 Replace the valve into its location.
Ensure that during the next steps the flexible cables close to the heat exchanger assemblies are not damaged.
3 Carefully insert the top plastic panel together with the Z-panel into the guide and press it half-way down.
4 Carefully plug the ambient temperature sensor into the rear of the top plastic panel.
Ensure that the ambient temperature sensor is completely plugged into the rear of the top plastic panel. Ensure that during the next steps the flexible cables close to the heat exchanger assemblies are not damaged.
84
5 Press the Z-panel together with the Top Plastic Panel completely down.
6 Press down completely until it clicks into its holding position.
7 Fix the Z-panel with the two screws.
8 Reconnect the grounding connection of the valve at the Z-panel.
Next steps:
9 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108. 10 Replace the column compartment into stack and reconnect capillaries and the power cable. 11 Turn on the column compartment.
85
Exchanging the Column Compartment Main (CCM) Board

When required Tools required If board is defective or for repair on other assemblies Screwdriver Pozidriv 1 PT3 Hexagonal wrench 5 mm Hexagonal wrench 7 mm Column compartment main board CCM G1316-69530 (exchange assembly)
Parts required
NOTE
The CCM board version G1316-66530/-69530 does not need the CID board anymore. The function of the CID board has been incorporated into the new CCM board. The minimum firmware for the new CCM board is A.05.05 and cannot be downgraded to firmware revisions below (is not required, since it works like all previous versions).
1 Switch off the column compartment, and disconnect the cables. 2 Remove column compartment from stack and place it on the working bench. 3 Remove the front cover, top cover and top foam section, see Installing the Foam and the Top Cover on page 108.
Remote
GPIB
Figure 16
Unscrew Connectors from Board
4 Use a 5 mm and 7 mm wrench to unscrew the REMOTE and the GPIB connector. 5 Disconnect all connectors from the processor board.
86
J2 - RS-232C J3 - REMOTE J4 - GPIB J5/25 - CAN J7 - Power Supply J11 - Column Switching Valve J14 - Heat Exchanger Assembly (left) J15 - Heat Exchanger Assembly (right) J16- Fan Assembly (right) J17 - Fan Assembly (left) J18 - Leak Sensor J19 - Flexboard (left) J21 - Flexboard (right) J23 - Column Identification Board (CID) B - Battery S - Configuration Switch
S B J17 J18 Safety lock
J4
J5 CID J23
J25 J11
J2
J3 J7 J14 J15
J16 J19 J21
Figure 17
Location of connectors
NOTE
The connector J11 for the CID board has been removed with TCC main board revision G1316-66530 in 2003. The CIDs function has been incorporated into the main board.
6 Remove the processor board. Place the board on the ESD kit. 7 In most cases the RFI spring plate remains on the interface connectors of the board. Carefully remove the spring plate and place it back into its position in the instrument before installing a new board. 8 On the new board check the switch setting of address switch S1, see Setting the 8-bit Configuration Switch on page 171.
NOTE
An incorrect switch setting (e.g., TEST/BOOT) may cause the module to turn in a basic mode (yellow or red flashing status light). In such a case turn off the module, re-set the address switches, and turn on the module again.
87
9 Install the new processor board and reconnect the connectors. 10 Refit the screws at the REMOTE and GPIB connectors. 11 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108. 12 Replace column compartment into the stack and reconnect the cables.
NOTE
If a new CCM board is installed, update the serial number information of the column compartment in the user interface, see. procedure below.
13 Check the firmware revision of the module. If the firmware revision is older than the current firmware revision of the module, update the firmware using the standard firmware update procedure, see Replacing the Column Compartments Firmware on page 112.
Entering the Serial Number using the Control Module

1 Connect the control module to the column compartment. Turn on the column compartment. 2 In the control module, press System (F5), then Records (F4). Using the up/down arrows, make sure that the column compartment is highlighted. 3 Press FW Update (F5). Now, press the m key. This will display a box which says Update Enter Serial#. 4 Press Enter. This will display the box labeled Serial#. 5 Letters and numbers are created using the up and down arrows. Into the box labeled Serial#, enter the 10-character serial number for the column compartment. When the 10-character serial number is entered, press Enter to highlight the complete serial number. Then, press Done (F6).
NOTE
For firmware revisions below A02.00 it is very important never to press Done if the Serial# box is blank. In this case, the module can no longer be recognized by either the control module or the ChemStation. The main board must then be replaced.
6 Turn the column compartment off, then on again. The Records screen should display the correct serial number for this module. 7 If a ChemStation is also connected, re-boot the ChemStation now as well.
88
Entering the Serial Number using the ChemStation

Module serial numbers are entered by typing specific commands into the command line at the bottom of the main user interface screen. 1 To enter a module serial number, type the following command into the command line: print sendmodule$(lthm, "ser YYYYYYYYYY") Where: YYYYYYYYYY is the 10-character serial number of the module in question.
NOTE
The first two characters are letters, which should be capitalized.
The reply line will respond with RA 0000 SER followed by the module serial number you just entered. 2 Turn off the column compartment, then on again. Then, re-boot the ChemStation. If the serial number you have just entered is different than the original module serial number, you will be given the opportunity to edit the configure 1100 access screen during the re-boot of the ChemStation. 3 After boot-up, the serial number you have just entered can be seen under the Instrument menu of the main user interface screen. The serial number of the column compartment can also be seen by typing the following command into the command line: print sendmodule$ (lthm, "ser?") The reply line will give the module serial number.
89
Replacing the Column Identification Module (CID) Board

The column identification module board (CID) is plugged onto the column compartment main (CCM) board.
When required Tools required If board is defective Screwdriver Pozidriv 1 PT3 Hexagonal wrench 5 mm Hexagonal wrench 7 mm Column identification module board (CID) G1316-66503
Parts required
NOTE
The CID board has been removed with TCC main board revision G1316-66530 in 2003. The CIDs function has been incorporated into the main board.
Preparations for this procedure: Turn off the column compartment. Disconnect the power cable. Disconnect capillaries. Remove column compartment from stack and place it on the working bench. Remove the front cover, top cover and top foam section, see Installing the Foam and the Top Cover on page 108.
1 Carefully exchange the CID board.
4 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108.
90
Exchanging the Fan

When required Tools required Parts required If the fan is defective or noisy or for repair of other assemblies Screwdriver Pozidriv 1 PT3 Fan assembly 3160-1017
CAUTION
The fan must be installed in the correct orientation to ensure optimum cooling and operation of the column compartment.
Preparations for this procedure: Turn off the column compartment. Disconnect the power cable. Disconnect capillaries. Remove column compartment from stack and place it on the working bench. Remove the front cover, top cover and top foam section, see Installing the Foam and the Top Cover on page 108.
1 Disconnect the fan assembly from the processor board.
91
2 Replace the fan assembly. Ensure correct fan orientation.
3 The arrow on the fan should correspond with the direction of air flow which is from the front to the rear of the column compartment.
4 Reconnect the fan assembly to the processor board and check that the cable of the left fan is correctly in the foam channel to assure correct closing of the foam parts.
5 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108.
92
Removing the Heat Exchanger Assemblies

When required Tools required Parts required If the heater is leaking, blocked or does not heat/cool or other assemblies have to be removed Screwdriver Pozidriv 1 PT3 Wrench 1/4 5/16 inch for capillary connections Heat exchanger assembly (left) G1316-60007, includes ambient temperature sensor Heat exchanger assembly (right) G1316-60006
Preparations for this procedure:

Turn off the column compartment. Disconnect the power cable. Disconnect capillaries. Remove column compartment from stack and place it on the working bench. Remove the front cover, top cover and top foam section, see Installing the Foam and the Top Cover on page 108.
1 Disconnect the heat exchanger cables from the processor board and the grounding connection of the column switching valve.
93
2 Carefully pull out the heater cables out of the foam channels.
3 Unscrew the Z-panel.
4 Press against the rear of the Z-panel to release the metal plate from the guide and pull it carefully upwards.
5 Lift the Z-panel together with the top plastic panel half-way out of the guide.
94
6 Locate the ambient temperature sensor in the top plastic part.
7 Carefully remove the ambient temperature sensor plugged into the rear of the top plastic panel.
8 Carefully pull out the top plastic panel together with the Z-panel completely.
9 Remove the heat exchanger assembly.
The repair level of the heat exchanger assemblies is the complete assembly.
95
Installing the Heat Exchanger Assemblies

When required Tools required Parts required If the heat exchanger is leaking, blocked or does not heat/cool or other assemblies have to be removed Screwdriver Pozidriv 1 PT3 Wrench 1/4 5/16 inch for capillary connections Heat exchanger assembly (left) G1316-60007, includes ambient temperature sensor Heat exchanger assembly (right) G1316-60006
1 Replace the leak tubing assembly into its location on leak base. Ensure that it keeps in this position during the next steps.
2 Replace the heat exchanger assembly. A screwdriver might be helpful to insert the silicon isolation.
Ensure that during the next steps the flexible cables close to the heat exchanger assemblies are not damaged.
96
3 Carefully insert the top plastic panel together with the Z-panel into the guide and press it half-way down.
4 Carefully plug the temperature sensor into the rear of the top plastic panel.
Ensure that the ambient temperature sensor is completely plugged into the rear of the top plastic panel. Ensure that the flexible cables close to the heat exchanger assemblies are not damaged.
5 Press the Z-panel together with the top plastic panel completely down. 6 Press down completely until it clicks into its holding position.
97
7 Fix the Z-panel with the two screws.
8 Reconnect the heat exchanger cables to the processor board and the grounding connection of the column switching valve.
9 Carefully replace the heat exchanger cables into the foam channels.
Next steps:
10 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108. 11 Replace the column compartment into stack. 12 Reconnect capillaries. 13 Reconnect the power cable. 14 Turn on the column compartment.
98
Exchanging the Power Supply

When required Tools required If defective Screwdriver Pozidriv 1 PT3 Wrench 1/4 inch Wrench 5 mm and 7 mm Power supply 0950-2528
Parts required
NOTE
The repair level of the power supply assembly is exchange of the complete assembly. No serviceable parts inside.
1 Switch off the column compartment, and disconnect the cables. 2 Remove column compartment from stack and place it on the working bench. 3 Remove the front cover, top cover and top foam section, see Removing the Top Cover and Foam on page 77. 4 Remove the column switching valve (if installed), see Removing the Column Switching Valve on page 80. 5 Remove the processor board, see Exchanging the Column Compartment Main (CCM) Board on page 86. 6 Remove the fan assemblies, see Exchanging the Fan on page 91. 7 Remove the heat exchanger assemblies, see Removing the Heat Exchanger Assemblies on page 93.
NOTE
The leak tubing assembly might fall out of its position.
8 Remove the leak sensor cable out of the foam channel.
99
15 Carefully remove the bottom foam.
16 Unscrew the power supply at the rear of the column compartment.
17 Press down the power switch light pipe to remove it from the coupler.
18 Remove the power supply completely. Re-use the coupler on the new power supply.
Power switch light pipe
Coupler
The repair level of the power supply assembly is exchange of the complete assembly. No serviceable parts inside.
100
19 Insert the power supply into its location and fix it with the screw at the rear panel.
20 Press down and clip in the power switch light pipe into the power supply.
Power switch light pipe
21 Reinstall bottom foam part.
Next steps:
22 Reinstall the processor board, see Exchanging the Column Compartment Main (CCM) Board on page 86. 23 Reinstall the fan assemblies, see Exchanging the Fan on page 91. 24 Reinstall the heat exchanger assemblies, see Installing the Heat Exchanger Assemblies on page 96. 25 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108. 26 Replace column compartment into the stack. 27 Reconnect the power cable and turn on the column compartment.
101
Replacing the Leak Sensor or Leak Base

When required Tools required If leak sensor is defective or leak base is damaged Screwdriver Pozidriv 1 PT3 Screwdriver flat blade Wrench 1/4 inch Hexagonal wrench 3 mm Leak sensor assembly 5061-3356 Leak base G1316-43101 (part of leak panel kit G1316-68700)
Parts required

Switch off the column compartment, and disconnect the power cable. Remove column compartment from stack and place it on the working bench. Remove the front cover, top cover and top foam section, see Removing the Top Cover and Foam on page 77.
1 Disconnect the leak sensor assembly from the processor board and pull the cable out of the foam channel.
102
2 Remove the column switching valve (if installed), see Removing the Column Switching Valve on page 80. 3 Remove the heat exchanger assemblies, see Removing the Heat Exchanger Assemblies on page 93.
4 Locate the clips.
5 Remove the clips (shown from the inside of the column compartment) on both sides using a flat screwdriver.
6 Remove the leak base from the cabinet by unlocking it with a flat blade on the right and left side of the leak base.
103
7 Remove the leak sensor assembly from the rear of the leak base.
8 Replace the leak sensor assembly into the leak base.
9 Route the leak sensor cable through the z-panel into the left foam channel.
10 Replace the leak base into the cabinet until it clicks into the cabinet. Insert the right side first.
104
11 Position the leak sensor so that it does not touch the bottom of the leak base.
12 Replace the clips (shown from the inside of the column compartment) on both sides using a flat screwdriver.
13 Replace the leak tubing assembly into its location on leak base. Ensure that it keeps in this position during the next steps.
14 Replace the heat exchanger assemblies, see Installing the Heat Exchanger Assemblies on page 96.
105
15 Reconnect the leak sensor and heat exchanger cables to the processor board and place the cables in the foam channel.
Next steps:
16 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108. 17 Replace the column compartment into the stack. 18 Reconnect the cables. 19 Turn on the column compartment.
106
Replacing Status Light Pipe

When required Tools required Parts required When part is broken Screwdriver Pozidriv 1 PT3 Status light pipe 5041-8384

Remove the front cover and top cover, see Removing the Top Cover and Foam on page 77.
1 The status light pipe is clipped into the top cover.
Next steps:
2 Replace the foam section, the top cover and front cover, see Installing the Foam and the Top Cover on page 108. 3 Replace the column compartment into the stack and reconnect the cables and capillaries. 4 Turn on the column compartment.
107
Installing the Foam and the Top Cover

When required Tools required Prerequisites When all repairs have been completed Screwdriver Pozidriv 1 PT3 The column compartment is open and other procedures have been carried out
1 Route the valve connector through the top foam and carefully fit the top foam into the column compartment.
2 Reconnect the valve connector to the main board.
Connector
108
3 Make sure that the foam is installed correctly and is located in the safety light switch.
4 Position of the foam in the safety light switch.
5 Place the top plate on the foam and slide it towards the rear and fix the screws at the rear of the top plate.
6 Place the top cover into the guides.
Ensure correct fit with Z-plane
109
7 Replace the cover.
8 Replace the front panel.
Next steps:
9 Replace the column compartment into the stack. 10 Reconnect the cables. 11 Turn on the column compartment.
110
Assembling the Main Cover

When required Tools required Parts required If cover was broken None Plastics kit G1316-68703 (includes base, top, left and right)
NOTE
The plastics kit contains all parts, but it is not assembled.
WA R N I N G
In case you insert the left or right side in the opposite position, you may not be able to remove the side from the top part.
1 Place the top part on the bench and insert the left and right side into the top part.
2 Replace the cover.
3 Replace the column compartment into the stack and reconnect the cables and capillaries. 4 Turn on the column compartment.
111
Replacing the Column Compartments Firmware

The installation of new firmware is required if new version solves problems of currently installed version. if after exchange of the processor board (CCM) the version on board is older than previous installed one. To upgrade the column compartments firmware the following steps have to be performed: 1 Load the firmware into the column compartment, see the help system of your user interface. 2 Perform a Temperature Calibration Procedure on page 58 to add the recalibration parameters into the boards memory (only necessary if a temperature calibration was done before). 3 If the CCM board was exchanged, re-enter the serial number information of the column compartment through the user interface, see Entering the Serial Number using the Control Module on page 88 or Entering the Serial Number using the ChemStation on page 89.
112
5 Identifying Parts and Materials

Overview of Main Assemblies 114 Sheet Metal Kit 120 Control Module 119 Sheet Metal Kit 120 Plastic Parts 121 Foam Parts 122 Power and Status Light Pipes 123 Leak Parts 124 Accessory Kit 125 Cable Overview 126 RS-232C Cable Kit 143 LAN Cables 144
113
Overview of Main Assemblies
3 2 1 4
4 5
Figure 18
Overview on Main Assemblies
114
Table 5
Item 1
Main Assemblies
Description Column compartment main board CCM (exchange part), requires minimum firmware A.05.05 does not need external CID board, Exchanging the Column Compartment Main (CCM) Board on page 86 Hexagonal nut for GPIB connector Hexagonal nut for RS-232 connector Cable CAN to Agilent 1100 Series modules Part Number G1316-69530
0380-0643 1251-7788 5181-1516 G1316-66503 0950-2528 3160-1017 G1316-60007 G1316-60006 0101-0920 5061-3356 See page 124 G1316-87303 G1316-68708 G1316-44103 See page 121 See page 120 See page 122
2 3 4 5 6 7 8 9
Column identification board CID Power supply assembly, additional power and status light parts, see page 123 Fan assembly Heater (left) Heater (right) Column switching valve (optional), additional column switching valve parts, see page 120 Leak sensor assembly Leak handling parts Low dispersion capillary (0.12 mm i.d., 70 mm) Capillary Kit Column Switching, see page 120 Cover plate (when switching valve is not installed) Front cover and plastic parts (housing) Sheet metal parts Foam parts
115
Column Switching Valve
Table 6
Item

Description Column switching valve kit, includes valve and Capillary Kit CSV Column switching valve (complete assembly) Cover plate (when switching valve is not installed) Capillary Kit Column Switching includes two capillaries (0.17mm i.d., 180 mm) and three capillaries (0.17 mm i.d., 90 mm) Part Number G1353-68700 0101-0920 G1316-44103 G1316-68708 G1313-87305 G1316-87300 1535-4857 0100-1850 0100-1851
1 2 3 4 5 6
Stator screws Stator Head Stator face Stator ring Rotor seal 3 grooves (Tefzel) Rotor seal 3 grooves (Vespel) Isolation seal Cover if Column Switching Valve is not installed
0100-1854 0100-1855 0100-1852 G1316-44103
116
6 5 4 3 2 1 Spring side shows to rear
Figure 19
117
2 position / 10 port Valve
Table 7
Item
2 position / 10 port Valve Valve Parts

Description Part Number
2PS/10 PT Valve kit includes: 2PS/10 PT valve Capillary kit Technical Note
G1316-68709
0101-1343 G1316-68711 G1316-90100
118
Control Module
Table 8
Item
Control Module Parts

Description Control Module, replacement part including cable Plastic Housing Kit, includes front, back and a clamp CAN cable Agilent 1100 module to control module Part Number G1323-67001 5062-8583 G1323-81600
NOTE
The control module G1323A was replaced by the G1323B in 1999. The G1323B comprises 2 MB memory instead of 1 MB on the older versions and the control of all 1100 modules intruded since then.
Figure 20
Control Module
119
Sheet Metal Kit
Table 9
Item
Sheet Metal Kit Parts

Description Sheet metal kit includes items 1, 2 and 3 Part Number G1316-68701 G1316-00600 G1316-09100 G1316-09102
4 5 6
RFI shield RFI spring side RFI spring bottom
5 4 6
Figure 21
Sheet Metal Kit Parts
120
Plastic Parts
Table 10
Item 1 2 3
Plastic Parts
Part Number G1316-68703 G1316-68704 5042-1381
Description Plastic kit, includes base, sides and top Front cover Name plate Agilent 1100 Series
NOTE
For correct assembling of the top and sides, see Assembling the Main Cover on page 111.
Figure 22
Plastic Parts
121
Foam Parts
Table 11
Item 1, 2 1 2
Foam Parts
Part Number G1316-68702
Description EPP foam kit, includes 1 and 2 Top Base
NOTE
Do not order the individual part numbers mentioned on the foam.
Figure 23
Foam Parts
122
Power and Status Light Pipes
Table 12
Item

Part Number 0950-2528 5041-8382 5041-8384 5041-8381 5041-8383
Description Power supply assembly
1 2 3 4
Power light pipe Status light pipe Power switch button Coupler for power supply actuator
3 2
Figure 24
123
Leak Parts
Table 13
Item 1 2, 3 4 5 6 7
Leak Parts
Part Number 5061-3356 G1316-68700 5041-8388 G1316-42300 0400-0002 G1316-60002 0890-1711
Description Leak sensor Leak Kit, includes leak top and leak base Leak funnel Leak funnel holder O-ring for ambient temperature sensor Waste assembly, includes complete Y-tubing assembly with leak funnel Waste tubing 1200 mm long (part of accessory kit)
2 6 4 3 5
Figure 25
Leak Parts
124
Accessory Kit
This kit contains some accessories and tools needed for the installation and repair of the column compartment. Table 14
Item
Accessory Kit Parts Listing

Part Number G1316-68705 5062-8588 9300-1408 5063-6526 G1316-87300 0100-0043 0100-0044 79814-22406
Description Accessory kit Column identification tag (blank) for re-ordering use (pack of 3) ESD wrist strap Column clip, quantity=2, for re-order use (pack of 6)
1 2 3 4
Capillary column-heat exchanger 90 mm lg, 0.17 i.d. (not assembled) contains items 2, 3 and 4 Ferrule front SST, quantity=2 Ferrule back SST, quantity=2 Fitting SST, quantity=2
Figure 26
Capillary (Column-Heat Exchanger) Parts
125
Cable Overview
WA R N I N G
Table 15
Type Analog cables
Cable Overview
Description 3390/2/3 integrators 3394/6 integrators Agilent 35900A A/D converter General purpose (spade lugs) Part Number 01040-60101 35900-60750 35900-60750 01046-60105 01046-60203 01046-60206 01046-60210 03394-60600
Remote cables
3390 integrator 3392/3 integrators 3394 integrator 3396A (Series I) integrator 3396 Series II / 3395A integrator, see page 134 3396 Series III / 395B integrator Agilent 1100 / HP 1050 modules / HP 1046A FLD HP 1046A FLD Agilent 35900A A/D converter HP 1090 liquid chromatographs Signal distribution module
03396-61010 5061-3378 5061-3378 5061-3378 01046-60202 01046-60202
126
Table 15
Type BCD cables
Cable Overview, continued

Description 3396 integrator General purpose (spade lugs) Part Number 03396-60560 G1351-81600 G1322-61600 5181-1516 5181-1519 G1323-81600 G1103-61611 10833A 10833B 34398A
Auxiliary CAN cables
Agilent 1100 Series vacuum degasser Agilent 1100 module to module, 0.5 m Agilent 1100 module to module, 1 m Agilent 1100 module to control module
External contacts GPIB cable
Agilent 1100 Series interface board to general purpose Agilent 1100 module to Agilent ChemStation, 1 m Agilent 1100 module to Agilent ChemStation, 2 m
RS-232 cable LAN cable
Agilent 1100 module to a computer This kit contains a 9-pin female to 9-pin female null modem (printer) cable and one adapter. Cross-over network cable (shielded, 3 m long), (for point to point connection) Twisted pair network cable (shielded, 7 m long) (for hub connections)
5023-0203 5023-0202
127
Analog Cables
One end of these cables provides a BNC connector to be connected to Agilent 1100 Series modules. The other end depends on the instrument to which connection is being made.
Agilent 1100 to 3390/2/3 Integrators
Connector 01040-60101
Pin 3390/2/3 1 2 3 4 5 6 7 8
Pin Agilent 1100 Shield
Signal Name Ground Not connected
Center
Signal + Connected to pin 6
Shield
Analog Connected to pin 4 Key Not connected
128
Agilent 1100 to 3394/6 Integrators
Pin 3394/6 1 2 3
Pin Agilent 1100
Signal Name Not connected
Shield Center
Analog Analog +
Agilent 1100 to BNC Connector
Connector 8120-1840
Pin BNC Shield Center
Pin Agilent 1100 Shield Center
Signal Name Analog Analog +
129
Agilent 1100 to General Purpose
Pin 3394/6 1 2 3
Pin Agilent 1100
Signal Name Not connected
Black Red
Analog Analog +
130
Remote Cables
One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent 1100 Series modules. The other end depends on the instrument to be connected to.
Agilent 1100 to 3390 Integrators
Pin 3390 2 NC 7 NC NC NC NC NC NC
Pin Agilent 1100 1 - White 2 - Brown 3 - Gray 4 - Blue 5 - Pink 6 - Yellow 7 - Red 8 - Green 9 - Black
Signal Name Digital ground Prepare run Start Shut down Not connected Power on Ready Stop Start request
Active (TTL)
Low Low Low
High High Low Low
131
Agilent 1100 to 3392/3 Integrators
Pin 3392/3 3 NC 11 NC NC NC 9
Active (TTL)
Low Low Low
High High Low Low
4 - Key
1 NC
132
Pin 3394 9 NC 3 NC NC NC 5,14 6 1 13, 15
Signal Name Digital ground Prepare run Start Shut down Not connected Power on Ready Stop Start request Not connected
Active (TTL)
Low Low Low
High High Low Low
NOTE
START and STOP are connected via diodes to pin 3 of the 3394 connector.
133
Agilent 1100 to 3396A Integrators
Pin 3394 9 NC 3 NC NC NC 5,14 1 NC 13, 15
Active (TTL)
Low Low Low
High High Low Low
Agilent 1100 to 3396 Series II / 3395A Integrators

Use the cable 03394-60600 and cut pin #5 on the integrator side. Otherwise the integrator prints START; not ready.
134
Agilent 1100 to 3396 Series III / 3395B Integrators
Pin 33XX 9 NC 3 NC NC NC 14 4 NC 13, 15
Active (TTL)
Low Low Low
High High Low Low
135
Agilent 1100 to HP 1050, HP 1046A or Agilent 35900 A/D Converters
Connector 5061-3378
Pin HP 1050 / 1 - White 2 - Brown 3 - Gray 4 - Blue 5 - Pink 6 - Yellow 7 - Red 8 - Green 9 - Black
Active (TTL)
Low Low Low
High High Low Low
Agilent 1100 to HP 1090 LC or Signal Distribution Module
Pin HP 1090 1 NC 4 7 8 NC 3
Active (TTL)
Low Low Low
High High Low Low
5 - Key
6 NC
136
Pin Universal
Active (TTL)
Low Low Low
High High Low Low
137
BCD Cables
One end of these cables provides a 15-pin BCD connector to be connected to the Agilent 1100 Series modules. The other end depends on the instrument to be connected to.

Connector G1351-81600 Wire Color Green Violet Blue Yellow Black Orange Red Brown Gray Gray/pink Red/blue White/green Brown/green not connected not connected Pin Agilent 1100 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 +5 V Low Signal Name BCD 5 BCD 7 BCD 6 BCD 4 BCD 0 BCD 3 BCD 2 BCD 1 Digital ground BCD 11 BCD 10 BCD 9 BCD 8 BCD Digit 20 80 40 10 1 8 4 2 Gray 800 400 200 100
138
Pin 3392/3 1 2 3 4 5 6 7 8 9 NC
Pin Agilent 1100 1 2 3 4 5 6 7 8 9 15
Signal Name BCD 5 BCD 7 BCD 6 BCD 4 BCD 0 BCD 3 BCD 2 BCD 1 Digital ground +5V
BCD Digit 20 80 40 10 1 8 4 2
Low
139
Auxiliary Cable
One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser. The other end is for general purpose.
Agilent 1100 Series Degasser to general purposes
Connector G1322-61600
Color White Brown Green Yellow Grey Pink
Pin Agilent 1100 1 2 3 4 5 6
Signal Name Ground Pressure signal
DC + 5 V IN Vent
140
CAN Cable
Both ends of this cable provide a modular plug to be connected to Agilent 1100 Series modules CAN-bus connectors.
Agilent 1100 module to module, 0.5 m Agilent 1100 module to module, 1 m Agilent 1100 module to control module
5181-1516 5181-1519 G1323-81600
141
External Contact Cable

5 10 15 1 6 11
One end of this cable provides a 15-pin plug to be connected to Agilent 1100 Series modules interface board. The other end is for general purpose.
Agilent 1100 Series Interface Board to general purposes
Connector G1103-61611
Color White Brown Green Yellow Grey Pink Blue Red Black Violet Grey/pink Red/blue White/green Brown/green White/yellow
Pin Agilent 1100 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Signal Name EXT 1 EXT 1 EXT 2 EXT 2 EXT 3 EXT 3 EXT 4 EXT 4 Not connected Not connected Not connected Not connected Not connected Not connected Not connected
142
RS-232C Cable Kit
This kit contains a 9-pin female to 9-pin female null modem (printer) cable and one adapter. Use the cable and adapter to connect Agilent Technologies instruments with 9-pin male RS-232C connectors to most PCs or printers.
Agilent 1100 module to PC RS-232C Cable Kit 34398A
Instrument
DCD RX TX DTR GND DSR RTS CTS RI DB9 Male 1 2 3 4 5 6 7 8 9 DB9 Female 1 2 3 4 5 6 7 8 9 DB9 Female
PC
DCD RX TX DTR GND DSR RTS CTS RI DB9 Male
143
LAN Cables
Recommended Cables
Description Cross-over network cable (shielded, 3 m long), (for point to point connection) Twisted pair network cable (shielded, 7 m long) (for hub connections)
Part number 5023-0203 5023-0202
144
6 Introduction to the Column Compartment

Introduction to the Column Compartment 146 System Overview 147 Column-Identification System 149 Column Switching Valve (Optional) 153 Electrical Connections 155 Instrument Layout 157 The Electronics 158 Column Compartment Main Board (CCM) 159 Firmware Description 164 Agilent 1100 Series Interfaces 166 Setting the 8-bit Configuration Switch 171 The Main Power Supply Assembly 176
145
The Agilent 1100 Series thermostatted column compartment is a stackable temperature-controlled column compartment for LC. It is available as standalone module or as a component of a Agilent 1100 Series system. It is used for heating and cooling to meet extreme requirements of retention time reproducibility. The main features of this column compartment are: Peltier heating and cooling from 10 degrees below ambient up to 80 C with high heating and cooling speeds for maximum application flexibility and stability, holds up to three 30-cm columns and optimized design gives minimum dead volumes and maximum efficiency, two independently programmable heat exchangers contribute volumes of only 3 and 6 l, electronic column-identification module as standard for GLP documentation of column type, and major column parameters, optional high-quality Rheodyne column switching valve with ceramic stator-face assemblies for prolonged lifetime. For specifications, see Performance Specifications on page 204.
146
System Overview
The Concept of Heating and Cooling

The design of this thermostatted column compartment uses column heating and cooling devices with Peltier elements. The solvent entering the column compartment is heated up or cooled down to a settable temperature with two low-volume heat exchangers (3 l on left side, 6 l on right side), made of a short piece of capillary 0.17 mm i.d. leading through a heat exchanger. The heat exchanger is designed such that it can function simultaneously as an air heater. The surface of the heat exchanger is shaped such that the area around the column is kept on similar temperature level as the liquid running through the column. This is done by thermal convection and radiation between the heat exchanger fins. With this design it is ensured that the column and the solvent flowing through it, are almost at the same temperature. Actual temperature control is accomplished at the heat exchanger. The solvent cools down or heats up on its transfer from the heating block to the column inlet. This depends on several factors: flow rate, setpoint temperature, ambient temperature and column dimensions. For example, temperature setpoint is 40 C. The heat-exchanger temperature is controlled at 40.8 C and at the column entry there could be 39.8 C. The actual temperature displayed on the user interface is always the derived temperature taken at the heat exchanger, corrected by the offset explained above.
NOTE
Default mode: heater actual, column offset Corrected mode: heater offset, column actual
Any type of heated column compartment brings one important consequence for column temperature equilibration. Before an equilibrium is reached the whole mass of column, column packing and solvent volume inside the column has to be brought to the selected temperature. This depends on several factors: flow rate, setpoint temperature, ambient temperature and column dimensions. The higher the flow rate, the faster the column equilibrates (due to thermostatted mobile phase).
147
Figure 27 shows a setpoint temperature of 40 C. Some time after entering the setpoint the heat exchanger has reached its temperature and the control activity starts. The TEMPERATURE NOT READY signal will be cancelled 20 seconds after the sensed temperature was within a range of 0.5 C of the setpoint (other values are settable from the user interface). However this does not mean that the column is at the correct temperature. The equilibration of the column can take longer. Stability of the high pressure readings is a good indication for equilibrium.
45
Heat exchanger temperature

40
Temperature (C)
35
Column temperature
30
25
Example for a flow rate of 5 ml/min of water
20 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
Time (min)
Figure 27
Equilibration of Heat Exchanger and Column Temperature
The temperature calibration and verification is described in Temperature Calibration Procedure on page 58.
148
Column-Identification System
The Agilent 1100 Series thermostatted column compartment is equipped with a column-identification system. This allows to write and to read column-specific information to and from the column-identification tag.
Antennas
Column clip
Figure 28
Column-Identification System
Table 16 shows the information that can be stored: Table 16

Item Product number Serial number Batch number
Column-Identification Module Information

Example 79916OD-552 950522 1675 Date of manufacturing Comment
149
Table 16
Item
Column-Identification Module Information, continued

Example 100 2.1 ODS Hypersil 10 m 1267 400 70 12 See Note below. Comment
Geometry [mm] Stationary phase Particle size Number of injections Maximum pressure allowed [bar] Maximum temperature recommended [C] Maximum pH recommended Column void volume [ml]
The number of injections will be updated each run to create a column lifecycle (history). The user interface allows to edit all information.
NOTE
If a column switching valve (see page 153) is installed in the module, the update of the number of injections depends on the position of the column switching valve. For example, if the left column is selected, the right column is not updated, and vice versa. If no column switching valve is installed both sides are updated at the same time.
Column-Identification Tag
When correctly placed on the heat exchanger, the distance between tag and antenna is 12 mm. This is the optimum distance for proper function. The identification tag can be easily removed from the column.
NOTE
For small diameter columns, a cable tie wrap should be used, to fix the column-identification tag at the column (in case you modify your own column). Assure that the tie wrap is not blocking the front cover.
150
NOTE
There is a difference in attaching the identification tag to the column depending on which heat exchanger it will be located, see Figure 29 on page 151 and Figure 30 on page 151. The Agilent logo should be always at the front side.
Figure 29
Column-Identification Tag for Left Heat Exchanger
Figure 30
Column-Identification Tag for Right Heat Exchanger
151
Column Clip
For better positioning of the column on the heat exchanger a column clip is available (part of accessory kit, Accessory Kit on page 125).
Figure 31
Column Clip
152
Column Switching Valve (Optional)
Figure 32
Location of Column Switching Valve
Two Column Selection

The valve can select either column 1 or column 2. The offline column is sealed by connecting head to rail. Switching should be done when the flow is off and the pressure is zero.
From autosampler Heater assembly 1 Heater assembly 2
Column 1 4
3 2
Column 2
1 6 To detector
Figure 33
Column 1 Active 153
Column 1 4
3 2
Column 2
1 6 To detector
Figure 34
Column 2 Active
Precolumn Back-flushing
The sample is injected into series-connected precolumn and analytical column. After the valve has switched, the analytical column flow continues in normal direction. Only the precolumn is back-flushed, eluting highly retained peaks directly to the detector.
Precolumn 4
3 2 1 6 To detector
Analytical Column
Figure 35
Precolumn Back-flushing
154
Electrical Connections
The GPIB connector is used to connect the column compartment with a computer. The address and control switch module next to the GPIB connector determines the GPIB address of your column compartment. The switches are preset to a default address (see Table 18 on page 167 or see Table 22 on page 172) and is recognized once after power is switched on. The CAN bus is a serial bus with high-speed data transfer. The two connectors for the CAN bus are used for internal Agilent 1100 Series module data transfer and synchronization. The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down, prepare, and so on. With the appropriate software, the RS-232C connector may be used to control the module from a computer through a RS-232C connection. This connector is activated and can be configured with the configuration switch next to the GPIB connector (see Communication Settings for RS-232C Communication on page 173). See your software documentation for further information The power input socket accepts a line voltage of 100120 or 220240 volts AC 10 % with a line frequency of 50 or 60 Hz. Maximum power consumption is 320 VA. There is no voltage selector on your column compartment because the power supply has wide-ranging capability. There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. The security lever at the power input socket prevents that the column compartment cover is taken off when line power is still connected.
WA R N I N G
155
Security lever
APG Remote RS-232C
CAN
GPIB
Power
Configuration switch
Figure 36
Electrical Connections
156
Instrument Layout
The industrial design of the column compartment incorporates several innovative features. It uses Agilents E-PAC for housing the electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers into which the mechanical and electronic boards components of the column compartment are placed. This is then housed in a metal inner cabinet which is then enclosed by a plastic external cabinet. The advantages of this packaging technology are: virtual elimination of fixing screws, bolts or ties, reducing the number of components and increasing the speed of assembly and disassembly, the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations, the plastic layers help cushion the electronic and mechanical parts from physical shock, and the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the column compartment.
157
The Electronics
The electronics are comprised of four main components: column compartment main board (CCM), see page 159. column identification board (CID), see Column-Identification Module on page 160 power supply, see page 176.
158
Column Compartment Main Board (CCM)
This board controls all information and activities of all assemblies within the column compartment. The operator enters parameters, changes modes and controls the column compartment through interfaces (CAN, GPIB, LAN or RS-232C), connected to the user-interfaces. Figure 37 on page 162 and Figure 38 on page 163 show block diagrams of this board.
ASIC Application Specific Integrated Circuit

The 304-pin application-specific integrated circuit (ASIC) provides interfacing to external devices through drivers, including GPIB, LAN, CAN, APG Remote. It is directly connected to the 4 control LEDs located near the connectors on this board and the 8-bit configuration switch which is used to configure the address for the GPIB communication, baud rate for RS-232C transfer, and so on. For switch settings, refer to Agilent 1100 Series Interfaces on page 166 and Setting the 8-bit Configuration Switch on page 171. In addition the ASIC controls and drives module specific functions and reads static status signals. It controls the cooling fans via the PWM (pulse width modulation) drivers. Movement of the cooling fans is sensed by the microprocessor.
Peltier Driver
There are two identical Peltier element drivers in the module. Their temperatures are controlled and measured on the hot and cold side of the Peltier elements by the micro-controller.
Valve Driver
One valve driver is used for the operation of an optional Column Switching Valve.
Electronic Fuses
The circuits that are connected to + 36 V are fused on the board electronically. This prevents the damage to components.
159
Column-Identification Module
The column-identification module (CID) is seated on the CCM board (see Overview on Repair Procedures on page 67) and allows to write and read column specific information (type, maximum pressure, number of injections, and so on, see Column-Identification System on page 149) to/from the column storage device via antennas in the heater assemblies. The number of injections will be updated each run to create a column lifecycle (history). The user interface allows to edit all information.
NOTE
The CCM board version G1316-66530/-69530 does not need the CID board anymore. The function of the CID board has been incorporated into the new CCM board. The minimum firmware for the new CCM board is A.05.05 and cannot be downgraded to firmware revisions below (is not required, since it works like all previous versions).
Diagnostic A/D Converter

The diagnostic A/D converter senses currents and voltages of the Peltier elements and converts the analog signals into digital values. When values are outside of the normal range, an appropriate error message is generated. It is also used for option identification.
Leak Converter
This block consists of a PTC (resistor with positive temperature coefficient) for the leak identification and a NTC (resistor with negative temperature coefficient) for the ambient temperature measurement. This ensures that temperature changes are not identified as leak. A leak would cool down the PTC and its change in resistance results into a leak signal. The signals are converted by the A/D converter.
Fan Drives
The variable revolution of the fans are controlled by the main processor depending on the internal heat distribution in the module. The fans provide a PWM signal which is proportional to the revolution. This fan status signal is used for diagnostics (defect recognition).
160
On-board Battery
An on-board lithium battery buffers the electronic memories when the column compartment is turned off. For safety information on lithium batteries see Lithium Batteries Information on page 211.
Interfaces
For detailed information on interfaces see Agilent 1100 Series Interfaces on page 166.
161
CCM
Main Control Functional Block Diagram J14 Peltier left J19 J19 Ambient Temp J15 Status LEDs 4 control LEDs 8-bit switch GPIB driver CAN driver Peltier drive R q J21 J18 Leak Sensor Peltier right
J11
Valve Driver
Peltier drive L q q
ASIC application specific integrated circuit
PWM driver
J17 Fan L
J4 J25 J5 J3
sense PWM driver J16 Fan R sense
REMOTE driver RS-232C driver Safety lock
J2
Main processor
Real time clock
Memory
J19 J21
Column ID -controller
J20
J7
MPS main power supply
Figure 37
Block Diagram Column Compartment Main Controller Functionality
162
CCM J14 Peltier L Temp Peltier drive L J19 + 24 V + 36 V
Ambient temperature J15 Peltier L temp Peltier drive R J21
+ 15V
Leak converter
J18
Leak sensor
+ 24 V + 36 V
Column Switching Valve
J11
+ 24V Valve drive + 15 V + 24V
J16 Fan drive R J17 Fan drive L Fan L Fan R
Column tag Colum n tag
J19 Antenna L Column id receiver and J21 transmitter Antenna electronics R J20 -controller + 15 V
+ 36 V electronic fuse
hardware is optional!
+ 36 V +24 V 15 V J7
+ 36 V
+5V
MPS main power supply
Figure 38
Interconnection Diagram Column Compartment Main Board
163
Firmware Description
The firmware of the instrument consists of two independent sections: a non-instrument specific section, called resident system, an instrument specific section, called main system.
Resident System
This resident section of the firmware is identical for all Agilent 1100 series modules. Its properties are: the complete communication capabilities (GPIB, CAN, LAN and RS-232C), memory management, ability to update the firmware of the main system.
Main System
Its properties are: the complete communication capabilities (GPIB, CAN, LAN and RS-232C), memory management, ability to update the firmware of the resident system. In addition the main system comprises the instrument functions that are divided into common functions like run synchronization via APG remote error handling, diagnostic functions and so on, or module specific functions like internal events such as heater control, column identification, and so on.
164
Firmware Updates
Firmware updates can be done using your user interface: hand-held control module with files from a PC-card or Agilent ChemStation with files from floppy disk The file naming conventions are: xxxx-vvv.DLB, where xxxxis the product number, e.g. 1316 for the G1316A TCC, and vvvis the revision number, for example 106 is revision 1.06 For instructions refer to your user interface.
NOTE
Update of main system can be done in the resident system only. Update of the resident system can be done in the main system only.
main FW update Resident System Main System
resident FW update
Figure 39
Firmware Update Mechanism
165
Agilent 1100 Series Interfaces
The Agilent 1100 Series modules provide the following interfaces: Table 17 Agilent 1100 Series Interfaces
Pumps Autosampler DA Detector MW Detector FL Detector Yes Yes Yes Yes 2 Yes VW Detector RI Detector Yes Yes Yes Yes 1 Yes Thermostatted Vacuum Column Degasser Compartment Yes Yes Yes Yes No No No No No Yes Yes* No
Interface Type
CAN GPIB RS-232C Remote Analog Interface board
Yes Yes Yes Yes Yes Yes
Yes Yes Yes Yes No Yes
* The vacuum degasser will have a special connector for specific use. For details see description of main board.
CAN connectors as interface to other Agilent 1100 Series modules, GPIB connector as interface to the Agilent ChemStation, RS-232C as interface to a computer, REMOTE connector as interface to other Agilent products, analog output connector(s) for signal output, and interface slot for specific interfacing (external contacts, BCD, LAN and so on). For identification and location of the connectors see Figure 5 on page 21.
WA R N I N G
Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations, see Cable Overview on page 126.
166
GPIB Interface
The GPIB connector is used to connect the module with a computer. The address and control switches next to the GPIB connector determine the GPIB address of your module. The switches are preset to a default address and recognized by the operating software from Agilent Technologies. Table 18 Default Addresses
28 22 23 24 25 26 27 Autosampler (HP 1050) Pump (HP 1050) VWD (HP 1050) DAD (HP 1050) 11 12 10 17 Autosampler RID 28 29
Autosampler Pump FLD VWD Agilent 8453A DAD/MWD Column Compartment
CAN Interface
The CAN is an intermodule communication interface. It is a 2 wire serial bus system supporting high speed data communication and real-time requirement.
Remote Interface
The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements. The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired-or technique).
167
To provide maximum safety within a distributed analysis system, one line is dedicated to SHUT DOWN the systems critical parts in case any module detects a serious problem. To detect whether all participating modules are switched on or properly powered, one line is defined to summarize the POWER ON state of all connected modules. Control of analysis is maintained by signal readiness READY for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal level are defined as: standard TTL levels (0 V is logic true, + 5 V is false) fan-out is 10, input load is 2.2 kOhm against + 5 V, and output are open collector type, inputs/outputs (wired-or technique). Table 19
Pin 1 2 3 4 5 6 7 8 POWER ON READY STOP
Remote Signal Distribution

Description Digital ground (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing preanalysis activities. (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities. (L) System has serious problem (for example, leak: stops pump). Receiver is any module capable to reduce safety risk. Not used (H) All modules connected to system are switched on. Receiver is any module relying on operation of others. (H) System is ready for next analysis. Receiver is any sequence controller. (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection). Receiver is any module performing run-time controlled activities. (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler.
Signal DGND PREPARE START SHUT DOWN
START REQUEST
168
RS-232C
The RS-232C connector is used to control the column module from a computer through RS-232C connection, using the appropriate software. This connector can be configured with the configuration switch module next to the GPIB connector. The RS-232C is designed as DCE (data communication equipment) with a 9-pin male SUB-D type connector. The pins are defined as: Table 20
Pin 1 2 3 4 5 6 7 8 9 In Out In In
RS-232C Connection Table

Function DCD RxD TxD DTR Ground DSR RTS CTS RI
Direction In In Out Out
169
Instrument
DCD RX TX DTR GND DSR RTS CTS RI DB9 Male 1 2 3 4 5 6 7 8 9 DB9 Female 1 2 3 4 5 6 7 8 9 DB9 Female
PC
DCD RX TX DTR GND DSR RTS CTS RI DB9 Male
Figure 40
RS-232 Cable
170
Setting the 8-bit Configuration Switch
The 8-bit configuration switch is located next to the GPIB connector. Switch settings provide configuration parameters for GPIB address, serial communication protocol and instrument specific initialization procedures.
Factory setting is shown for column compartment
Figure 41
8-bit Configuration Switch
Table 21
8-bit Configuration Switch

1 0 0 1 1 2 0 1 0 1 Baudrate Reserved RSVD SYS RSVD RSVD FC 3 4 5 6 7 8
Mode Select GPIB RS-232C Reserved TEST/BOOT
GPIB Address Data Bits Parity
171
Switches 1 and 2 define which set of parameters (for example, for GPIB, RS-232C, and so on) will be changed. Once the change has been completed, the instrument must be powered up again in order to store the values in the non-volatile memory. Here the parameters are kept, independently if you turn the column compartment off and on again. They will be kept until the same set of parameters is subsequently changed and power is reset. All other previously stored configuration settings are still being kept in non-volatile memory. In this manner you can store more than one set of parameters, for example, for GPIB and RS-232C, using the same 8-bit configuration switch twice.
GPIB Default Addresses

If you just want to change the GPIB address and need a detailed procedure, refer to the Installing Your Agilent ChemStation System handbook. Default GPIB address is set to the following addresses: Table 22
Module Pump FLD VWD Agilent 8453A DAD/MWD Column compartment Autosampler RID
Default Addresses for Agilent 1100 Series Modules

Address 22 23 24 25 26 27 28 29 Binary Address 00010110 00010111 00011000 00011101 00011010 00011011 00011100 00011101
where 0 means that the switch is down and 1 means that the switch is up.
172
Communication Settings for RS-232C Communication

The communication protocol used in the column compartment supports only hardware handshake (CTS/RTR). Switches 1 in down and 2 in up position define that the RS-232C parameters will be changed. Once the change has been completed, the column instrument must be powered up again in order to store the values in the non-volatile memory. Table 23
Mode Select RS-232C
Communication Settings for RS-232C Communication

1 0 2 1 3 Baudrate 4 5 6 Data Bits 7 Parity 8
Use the following tables for selecting the setting which you want to use for RS-232C communication. The number 0 means that the switch is down and 1 means that the switch is up. Table 24
Switches 3 0 0 0 0 4 0 0 1 1 5 0 1 0 1 9600 1200 2400 4800
Baudrate Settings
Baud Rate Switches 3 1 1 1 1 4 0 0 1 1 5 0 1 0 1 9600 14400 19200 38400 Baud Rate
Table 25
Switch 6 0 1
Data Bit Settings

Data Word Size 7 Bit Communication 8 Bit Communication
173
Table 26
Switches 7 0 1 1 8 0 0 1
Parity Settings
Parity
No Parity Odd Parity Even Parity
One start bit and one stop bit are always used (not selectable). Per default, the module will turn into 19200 baud, 8 data bit with no parity.
Forced Cold-Start Settings

Switches 1 and 2 do not force storage of this set of parameters in non-volatile memory. Returning the switches 1 and 2 to other positions (other than being both up) will allow for normal operation.
CAUTION
A forced cold start erases all methods and data stored in non-volatile memory. Exceptions are diagnose and repair logbooks which are saved from being erased.
If you use the following switch settings and power the column compartment up again, a forced cold start has been completed. Table 27 Forced Cold Start Settings
1 1 2 1 3 0 4 0 5 0 6 0 7 0 8 1
Mode Select TEST/BOOT
To return to normal operation, set switches back to your GPIB or RS 232C configuration settings.
174
Stay Resident Settings

Firmware update procedures may require this mode in case of firmware loading errors. Switches 1 and 2 do not force storage of this set of parameters in non-volatile memory. Returning the switches 1 and 2 to other positions (other than being both up) will allow for normal operation. If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident part, that is, it is not operable as a column compartment. It only uses basic functions of the operating system, for example, for communication. Table 28 Stay Resident Settings
1 1 2 1 3 0 4 0 5 1 6 0 7 0 8 0
Mode Select TEST/BOOT
To return to normal operation, set switches back to your GPIB or RS 232C configuration settings.
175
The Main Power Supply Assembly
The main power supply comprises a closed assembly (no on-site repair possibility). The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors. The line voltage can vary in a range from 100 120 or 220 240 volts AC 10 % and needs no manual setting.
M P S + 3 6 V s u p p ly + 3 6 V (n o t u s e d )
lin e v o lta g e
p rim a ry c irc u its
+ 2 4 V s u p p ly
+ 2 4 V / 4 .5 A
se n se
+ 5 V s w itc h in g re g u la to r
+ 5 V / 3 A
lin e a r v o lta g e re g u la to rs
+ 1 5 V / .3 A -1 5 V / .3 A
Figure 42
Main Power Supply (MPS) Block Diagram
WA R N I N G
To disconnect the instrument from line, unplug the power cord. The power supply still uses some power, even if the power switch on the front panel is turned off.
176
No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines. When overload conditions occur, the power supply turns off all output voltages. Turning the line power off and on again resets the power supply to normal operation if the cause of the overload condition has been removed. An over-temperature sensor in the main power supply is used to turn off output voltages if the temperature exceeds the acceptable limit (for example, if the cooling fan of the instrument fails). To reset the main power supply to normal operating conditions, turn the instrument off, wait until it is approximately at ambient temperature and turn the instrument on again. The following table gives the specifications of the main power supply. Table 29 Main Power Supply Specifications
130 W 100 120 or 220 240 volts AC 10 %, line frequency of 50/60 Hz + 24 V / 4.5 A (maximum) + 36 V / 2.5 A (maximum) +5V/3A + 15 V / 0.3 A - 15 V / 0.3 A Continuous output Wide ranging total power consumption of + 24 V and + 36 V must not exceed 107 W.
Maximum power Line Input Output 1 Output 2 Output 3 Output 4 Output 5
177
178
7 Control Module Screens for the Agilent 1100 Column Compartment

Major keys on the Agilent 1100 Control Module 180 Screens available from the Analysis screen 181 Screens available from the System screen 192 Screens available from the Records screen 194 Diagnostics and Tests 201
179
Control Module Screens for the Agilent 1100 Column Compartment
Major keys on the Agilent 1100 Control Module
Table 30
ESC m i Enter On/Off Start Plot Views Return to previous screen and scroll through top layer views (Analysis, Settings) Open context sensitive menus Information/help Store changed parameters or execute the choice from a pull-down menu Switch on heater(s) Start a run View the chromatogram Change between view of analysis - status - system screens
NOTE
The screens shown on the next pages are based on the following firmware revisions: Control Module firmware revision B.01.01 (G1323B). LC Module firmware revision 3.8x
NOTE
In case the control modules display seems to be frozen (hang-up due to a communication problem on the CAN bus, unplug the control module from the LC module and reconnect.
180
Screens available from the Analysis screen
The Analysis screen

This is the wake-up screen, if the Agilent 1100 thermostatted column compartment is the only configured Agilent 1100 module. It is used to enter the most common TCC method parameters. If no column switching valve (optional) is installed, the part is grayed out.
The m-key allows access to the context sensitive menu. Setup view leads you to add sections for additional Agilent 1100 modules. Restart re-boots the control module.
181
Heater ON/OFF
Use the F8 key (On/Off) to turn on the heater(s). If more than one module is available, select the F7 key (Temp).
Setup View
In the Setup view, e.g. another module can be added to the view.
182
Here, e.g. the variable wavelength detector parameters are shown on the display as well. The number of parameters on the display are restricted as additional modules are added. Maximum 4 modules are shown automatically. If more modules are in the system, you have to chose in Setup view.
With the Settings key you open a pull-down menu where you can select the column compartment module.
183
Settings
Within the Settings you can change the TCC parameters and with a different set of parameters available through the F1-5 keys. The F3 key is only available when the column switching valve is installed (optional). F7 key resets the TCC to default values. F8 opens a window to turn on the heater(s).
Use the m-key for the context sensitive menu. The Status command pulls up a monitor screen displaying signals and spectra as programmed. Reset will load the TCC default parameters.
Use F1-key (More). You can enter special TCC setpoints that enables the analysis either with any temperature or within a specified range.
184
Settings - Timetable
With the F2 key (Timetable) you can list the timetable for the TCC. Press F7 key (Insert) to add entries or F6 key (Delete) to remove entries.
Press the F7 key (Insert) to add a timetable events. Use the F6 key (Done) to view the entered lines of the timetable.
Use the m-key for the context sensitive menu. It gives you additional tools for the timetable.
185
Settings - Column Switching Valve

With the F3 key (Column Switch) you have access to the column switching valve, see Column Switching Valve (Optional) on page 153. You can select either the left or right column.
186
Settings - Column ID
With the F4 key (Column ID) you have access to the column id tag information, see Column-Identification System on page 149. Pressing F1 key (More) shows the rest of the parameters (inclusive # of injections). You can overwrite the information and press F7 key (Write) to transfer the new information into the column tag. F8 key (Right) changes to the right column id tag information (if installed).
187
Settings - Run times

With the F5 key (Runtimes) you can change the stop time and the post-run time.
Press F5 key (Views) and select Status.
188
Status
This is an example if an Agilent 1100 TCC is configured standalone. Information on the actual temperature setting (left and right), column name, elapsed run time, messages and the signal plot are shown. Press key F8 (Start) to start a run, key F7 (Rescale) to maximize the signal.
Signal plot
Press F6 key (Plot) to enter the plot screen (available also from the Analysis and System screen). Here you can observe the online signal(s). To add additional online signals (maximum 3), press F6 key (Select). If more than one signal is configured (see next), use the 1-2-3 number key to switch between the signals.
189
Press F6 key (Select). Here you can add additional online signals (maximum are 3). Additional signals could be also pressure or detector signals from other modules. Use the Right/Left arrows to switch between Available and Selected Signals. Use the F8 key (Move) to enter available signals into the box for selected signals or vice versa.
Method screens
On the Analysis screen use the F3 key (Method) to view the parameters in a method and F8 key (Save As) to save the method in the module(s). The PC-Card key is only active when a PCMCIA card is inserted in the control module.
190
Use F2 key (PC-Card) to save a method on a PCMCIA card. Use the Right/Left arrows to switch between PC-Card and Instrument window. Use the UP/Down arrows to select the method. Use the F7/F8 keys (Copy) to enter available signals into the box for selected signals or vice versa.
191
Screens available from the System screen
System screen
Use the Esc key to receive Views on the F5 key. Choose System from the pull-down menu. This screen shows the last activities in the system.
System - Control
Use the F1 key (Control) to select the TCC. Here you receive information about the not-ready conditions if needed. F2 key (Reset) does a re-initialization of the TCC.
192
System - Configuration
On the System screen use the F2 key (Configure) to select the TCC. Here you define further special setpoints for the TCC operation. The option line informs about a column switching valve, if installed (optional),.
Use the F1 key (Interfaces) to access the interface settings (if required).
193
Screens available from the Records screen
Records screen
Use the Esc key to receive Views on the F5 key. Choose System from the pull-down menu. Use the F4 key (Records) to select the TCC. Errors are reported either into the System Log (F2) or Error Log (F3).
System / Error Log

Use the F2 key (System Log) or F3 key (Error Log) to look for errors.
194
Info Log
Use the m-key to receive a pop-up menu, Select Info Log. A list of the last events are listed. For troubleshooting reasons they can be printed or saved to a file on the PCMCIA card (using the m-key for the context sensitive menu).
EMF (Early Maintenance Feedback)

Using the F1 key (EMF) enters the EMF section. There are no EMF setting possible on the TCC.
195
196
Firmware Update
Use the Esc key to receive Views on the F5 key. Choose System from the pull-down menu. Use the F3 key (Records) to select the TCC. Use the F5 key (FW Update) to enter the Update section. If you want to update the resident firmware (together with specific main firmware revisions), select the a file from the PCMCIA card (RESnnnn.DLB) and press execute. If you want to update the main firmware, press F7 key (Transfer) to turn the module into the resident mode (LED on module should blink yellow).
Use the Esc key to receive Views on the F5 key. Choose System from the pull-down menu. Use the F3 key (Records) to select the Generic module. In this screen the resident firmware revision is shown.
197
Use the F5 key (FW Update) to enter the Update section. Select the a file from the PCMCIA card (1316nnnn.DLB) and press execute. When the update has finished, press F7 key (Transfer) to return the module into the normal mode (LED on module should stay yellow).
If you have not saved your methods, please do it before continuing. Otherwise they will be overwritten during the update process.
198
Changing the serial number

In case the serial number of the module has to be added, use the m-key to open the menu Enter Serial#. The serial number becomes active after restart of the module.
Maintenance activities
On the Records screen use the F4 key (Maint log) to view and edit the maintenance logbook.
199
Use the F7 key (Add) to add new maintenance activities. If an activity is not listed, you can type the activity into the line Add using the control modules key pad.
200
Diagnostics and Tests
Tests screen
Use the Esc key to receive Views on the F5 key. Choose System from the pull-down menu. Use the F3 key (Tests) to select the TCC. F7 key (Reset) resets the module and F6 key (Plot) opens the plot window.
Calibration
With F1 key (Calibrate) the recalibration of the TCC can be accessed. Refer to Temperature Calibration on page 57 for more information before starting this function. with F8 key (Calibrate).
201
202
8 Specifications
Performance Specifications 204
203
Specifications
Performance Specifications
Table 31
Type
Performance Specifications Thermostatted Column Compartment

Specification 10 degrees below ambient to 80 C 0.15 C 0.8 C 0.5 C Three 30 cm 5 minutes from ambient to 40 C 10 minutes from 40 20 C 3 l left heat exchanger 6 l right heat exchanger 140 410 435 mm (5.5 16 17 inches) 10.2 kg (22.5 lbs) Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional i.d. 0.17 mm With calibration Comments
Temperature range Temperature stability Temperature accuracy Column capacity Warm-up/cool-down time Dead volume Dimensions (h w d) Weight Communications
Safety and maintenance Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. GLP features Column-identification module for GLP documentation of column type, see Column-Identification System on page 149 All materials recyclable.
Housing
204
Specifications
NOTE
All specifications are valid for distilled water at ambient temperature (25 C), set point at 40 C and a flow range from 0.25 ml/min.
205
Specifications
206
A Safety Informations
General Safety Information 208 Lithium Batteries Information 211 Radio Interference 212 Sound Emission 213 Solvent Information 214 Agilent Technologies on Internet 216
207
Safety Informations
General Safety Information
The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.
General
This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards. This instrument is designed and certified as a general purpose laboratory instrument for research and routine application only. It is not certified for in-vitro or medical applications.
Operation
Before applying power, comply with the installation section. Additionally the following must be observed. Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto-transformers, and devices connected to it must be connected to a protective earth via a ground socket. Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury. Whenever it is likely that the protection has been impaired, the instrument must be made inoperative and be secured against any intended operation. Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuseholders must be avoided.
208
Safety Informations
CAUTION
The operator of this instrument is advised that if the equipment is used in a manner not specified in this manual, the protection provided by the equipment may be impaired.
Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed. Energy available at many points may, if contacted, result in personal injury. Any adjustment, maintenance, and repair of the opened instrument under voltage should be avoided as much as possible. When inevitable, this should be carried out by a skilled person who is aware of the hazard involved. Do not attempt internal service or adjustment unless another person, capable of rendering first aid and resuscitation, is present. Do not replace components with power cable connected. Do not operate the instrument in the presence of flammable gases or fumes. Operation of any electrical instrument in such an environment constitutes a definite safety hazard. Do not install substitute parts or make any unauthorized modification to the instrument. Capacitors inside the instrument may still be charged, even though the instrument has been disconnected from its source of supply. Dangerous voltages, capable of causing serious personal injury, are present in this instrument. Use extreme caution when handling, testing and adjusting.
209
Safety Informations
Safety Symbols
Table 32 shows safety symbols used on the instrument and in the manuals. Table 32
Symbol
Safety Symbols
Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up.
WA R N I N G
A warning alerts you to situations that could cause physical injury or damage to the equipment. Do not proceed beyond a warning until you have fully understood and met the indicated conditions.
CAUTION
A caution alerts you to situations that could cause a possible loss of data. Do not proceed beyond a caution until you have fully understood and met the indicated conditions.
210
Safety Informations
Lithium Batteries Information
WA R N I N G
Danger of explosion if battery is incorrectly replaced. Replace only with the same or equivalent type recommended by the equipment manufacturer. Lithium batteries may not be disposed-off into the domestic waste. Transportation of discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries.
WA R N I N G
Lithiumbatteri - Eksplosionsfare ved fejlagtig hndtering. Udskiftning m kun ske med batteri af samme fabrikat og type. Lever det brugte batteri tilbage til leverandren.
WA R N I N G
Lithiumbatteri - Eksplosionsfare. Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten. Brukt batteri returneres appararleverandoren.
NOTE
Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien maar inleveren als KCA.
211
Safety Informations
Radio Interference
Manufacturers Declaration
This is to certify that this equipment is in accordance with the Radio Interference Requirements of Directive FTZ 1046/1984. The German Bundespost was notified that this equipment was put into circulation, the right to check the series for compliance with the requirements was granted.
Test and Measurement

If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
212
Safety Informations
Sound Emission
Manufacturers Declaration
This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. Sound Pressure Lp < 70 dB (A) At Operator Position Normal Operation According to ISO 7779:1988/EN 27779/1991 (Type Test)
213
Safety Informations
Solvent Information
Observe the following recommendations on the use of solvents.
Flow Cell
Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair the optical properties of the flow cell. Prevent any crystallization of buffer solutions. This will lead into a blockage/damage of the flow cell. If the flow cell is transported while temperatures are below 5 degree C, it must be assured that the cell is filled with alcohol. Aqueous solvents in the flow cell can built up algae. Therefore do not leave aqueous solvents sitting in the flow cell. Add small % of organic solvents (e.g. Acetonitrile or Methanol ~5%).
Solvents
Brown glass ware can avoid growth of algae. Always filter solvents through 0.4 m filters, small particles can permanently block the capillaries. Avoid the use of the following steel-corrosive solvents: Solutions of alkali halides and their respective acids (for example, lithium iodide, potassium chloride, and so on). High concentrations of inorganic acids like nitric acid, sulfuric acid especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel). Halogenated solvents or mixtures which form radicals and/or acids, for example: 2CHCl3 + O2 2COCl2 + 2HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.
214
Safety Informations
Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides. Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1-% solution of acetic acid in methanol will attack steel. Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). Mixtures of carbon tetrachloride with 2-propanol or THF.
215
Safety Informations
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products - Chemical Analysis It will provide also the latest firmware of the Agilent 1100 series modules for download.
216
Index
Numerics
2 position / 10 port Valve, 118 column capacity and length, 146 changing column and tags, 69 equilibration time, 148 column clip, 152 column ID settings, 187 column identification, 149 antenna and tag, 149 how to install tags, 150 tag, 150 what information, 149 column switching valve settings, 186 column switching valve (optional) description, 153 installing, 83 precolumn backflushing, 154 two column selection, 153 configuration switch default settings, 171 description and factory settings, 171 connector locations, 21 control module column id settings, 187 EMF, 195 enter serial number, 199 firmware update, 197 part number, 119 serial number change, 199 temperature calibration, 201 tests, 201 control module screen column switching valve, 186 controller. see CCM board cool-down time, 204 cooling concept, 147 coumn switching valve (optional) location, 153
D
dead volume, 204 delivery checklist, 15 dimensions, 14, 204
A
accessory kit, 125 installation, 16 accuracy of temperature, 204 Agilent on internet, 216 algea information, 214 antenna, 149
E
electrical connections descriptions of, 155 location of connectors, 156 EMF on control module, 195 equilibration time, 148 error messages, 30 column temperature, 52 compensation sensor open, 41 compensation sensor short, 42 cover violation, 46 defective heater circuit, 54 defective sensor, 49 defective temperature sensor, 49 heater profile, 50 heatsink temperature, 53 introduction, 33 leak, 38 leak sensor open, 39 leak sensor short, 40 left fan failed, 43 left temperature timeout, 47 open cover, 45 remote time out, 36 right fan failed, 44 right temperature timeout, 48 shutdown, 35 synchronization lost, 37 time-out, 34 valve failed, 51 ESD (electrostatic discharge) strap, 66 exchanging parts. see repairs external measuring device information, 58
B
battery description, 161 location on CCM board, 87 safety information, 211 boards column identification (CID), 160 location of connectors, 87 main board (CCM), 158
C
cable connecting APG Remote, 19 connecting CAN, 19 connecting ChemStation, 19 connecting GPIB, 19 connecting the power, 19 overview and identification, 126 calibration control module screen, 201 calibration, see temperature calibration CAN interface, 167 CCM board description, 158 CID board, 160 location, 67 replacing, 90
217
Index
F
features column identification, 146 column switching valve, 146 GLP, 204 instrument layout, 157 safety and maintenace, 204 firmware main system, 164 resident system, 164 updates, 165 firmware update with control module, 197 front view of module, 20 function test, 55 description, 55 failed, 56 profile, 55 fuses, 177
instrument layout, 157 interfaces APG remote, 167 CAN, 167 GPIB, 167 RS-232C, 169 internet, 216 introduction concept of heating and cooling, 147 system overview, 147 to column compartment, 27
O
operation temperature, 14 operation, theory of electronics, 158 optimizing the performance, 28
P
parts identification, 114 accessory kit, 125 cable overview, 126 cables - analog, 128 cables - auxiliary, 140 cables - BCD, 138 cables - external contact, 142 cables - LAN cables, 144 cables - remote, 131 column switching valve, 120 control module, 119 foams, 122 leak panels, 124 main assemblies, 114 plastics, 121 power and status, 123 sheet metal kit, 120 performance optimizing, 28 specifications and features, 204 physical specifications, 14 humidity, 14 line voltage and frequency, 14 operation temperature, 14 power consumption, 14 weight and dimensions, 14 power consumption, 14 power settings (autoranging), 176 power supply description, 176
L
LAN cables, 144 leaks, correcting, 74 limits of temperature calibration, 58 line voltage and frequency, 14
G
GLP features, 204 GPIB default addresses, 172 interface, 167
M
maintenance (simple repairs), 68 message column temperature, 52 compensation sensor open, 41 compensation sensor short, 42 cover violation, 46 defective heater circuit, 54 defective sensor, 49 defective temperature sensor, 49 heater profile, 50 heatsink temperature, 53 leak, 38 leak sensor open, 39 leak sensor short, 40 left fan failed, 43 left temperature timeout, 47 open cover, 45 remote time out, 36 right fan failed, 44 right temperature timeout, 48 shutdown, 35 synchronization lost, 37 time-out, 34 valve failed, 51
H
heat exchanger equilibration time, 148 heating concept, 147 humidity, 14
I
information on batteries, 211 on external measuring device, 58 installation accessory kit, 16 capillaries and waste tubings, 25 column, 24, 25 delivery checklist, 15 flow connections, 23 module, 20 of column switching valve, 83 temperature sensor, 60 unpacking, 15
R
rear view of module, 21 remote (APG) interface, 167 Removing the Top Cover and Foam, 77 repairs assembling main cover, 111 changing the column, 69 cleaning the instrument, 65 correcting leaks, 74
218
Index
exchanging CID board, 90 exchanging column switching valve parts, 72 exchanging processor board (CCM), 86 exchanging the fan, 91 installing column switching valve, 83 installing foam and top cover, 108 installing heat exchangers, 96 internal parts, 75 introduction, 64 overview, 67 removing column switching valve, 80 removing heat exchangers, 93 removing top cover and foam, 77 replacing leak sensor and leak base, 102 replacing status light pipe, 107 simple repairs overview, 68 types simple/internal, 64 using the ESD strap, 66 warnings and cautions, 64 replacing parts. see repairs RS-232C cable kit to PC, 143 communication settings, 173 interface, 169
location, 31 system overview, 147
T
tag, 149 installation, 150 temperature accuracy, 204 range, 204 stability, 204 temperature calibration, 30 control module screen, 201 cross-over point, 57 description limits, 58 problems, 59 procedure, 58 temperature verification, 30 external measuring device, 58 principle, 60 test thermostat function test, 30, 55 thermostat function test failed, 56 tests on control module, 201 troubleshooting error messages, 30 status indicators, 30
S
safety information on lithium batteries, 211 standards, 14 serial number entered on ChemStation, 89 entered on control module, 88, 199 serial number change with control module, 199 setting the addresses, 171 site requirements, 12 bench space, 13 environment, 13 power considerations, 12 power cords, 12 specifications, 204 stack configuration, 17 front view, 18 rear view, 19 status indicators, 30, 31 details, 31
U
unpacking, 15
V
verification, see temperature verification
W
warm-up time, 204 weight, 14, 204
219
Index
220
www.agilent.com
In This Book
This manual contains technical reference information about the Agilent 1100 Series thermostatted column compartment. The manual describes the following: installing the column compartment, the column compartment optimization, diagnostics and troubleshooting, repairing the column compartment, parts and materials, introduction to and theory of operation, and screens of local control module.
Agilent Technologies 1995, 1996-2004

Printed in Germany Edition 05/2004
*G1316-90004* *G1316-90004* G1316-90004


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1100 Series Thermos Tatted Column Compartment Reference Manual G1316-90004

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Agilent 1100 Series Thermostatted Column Compartment

Manual Part Number

Restricted Rights Legend

1100 Series TCC Reference Manual

How to optimize the Column Compartment

Troubleshooting and Test Functions

Repairing the Column Compartment

Identifying Parts and Materials

Introduction to the Column Compartment

Control Module Sceens for the Agilent 1100 Column Compartment

1100 Series TCC Reference Manual

1100 Series TCC Reference Manual

1100 Series TCC Reference Manual

1100 Series TCC Reference Manual

1100 Series TCC Reference Manual

Column-Identification System Column-Identification Tag Column Clip 152

1100 Series TCC Reference Manual

Performance Specifications A Safety Informations 207

Agilent Technologies on Internet Index 217

1100 Series TCC Reference Manual

1100 Series TCC Reference Manual

Agilent 1100 Series Thermostatted Column Compartment Reference Manual

1 Installing the Column Compartment

Installing the Column Compartment

A suitable environment is important to ensure optimum performance of the column compartment.

1100 Series TCC Reference Manual

Installing the Column Compartment

1100 Series TCC Reference Manual

Installing the Column Compartment

1100 Series TCC Reference Manual

Installing the Column Compartment

Unpacking the Column Compartment

Column Compartment Delivery Checklist

1100 Series TCC Reference Manual

Installing the Column Compartment

Accessory Kit Contents

Accessory Kit Contents (Part number G1316-68705)

Ferrule front Ferrule back Fitting Capillary

Capillary (Column-Heat Exchanger) Parts

1100 Series TCC Reference Manual

Installing the Column Compartment

Optimizing the Stack Configuration

1100 Series TCC Reference Manual

Installing the Column Compartment

Autosampler Control module

Recommended Stack Configuration (Front View)

1100 Series TCC Reference Manual

Installing the Column Compartment

Remote cable Analog pressure signal

CAN Bus cable

CAN Bus cable

AC power Analog signal to recorder GPIB or LAN to LC ChemStation

Recommended Stack Configuration (Rear View)

1100 Series TCC Reference Manual

Installing the Column Compartment

Installing the Column Compartment

Status indicator green/yellow/red

Line power switch with green light

Front View of the Thermostatted Column Compartment