Scil ABC Vet User Manual

ABC VetUserManual
Part number: RAB 015A Ind.E
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REVISIONS
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A B RAH533 AA RAH569 AA RAH536 AA RAH634 AA RAH653 AA X Creation version V1.0 Software
all all 15.12.96 19.06.98
V1.42 version Update software ABCVet16 parameters creck c erwi Electromagneti ronment V210 version soltware Update V2.21 version software Update of Newcertificate conformity
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DECLARATION CON FORMITE DE DECLARATION CON FORMITY OF

ACcoRDrRit% EN45014
DIAG N OSTI CS
ParcEurom6decine ruedu Caduc6e BP7290 MONTPELLIER 34184 4 Cedex FRANCE
NOUSLE FABRICANT WETHEMANUFACTURER
*X{ABXDiagnostics
Adresse ParcEurom6decine Address ruedu Caduc6e BP7290 34184MONTPELLIER Cedex 4 FRANCE DECLARONS OUELE PRODUIT DECLARE THATTHE PRODUCT
Nomproduit Productname Moddles Models
MICROS
OT-CTOS-CS-A Vet-CHOR B C |
DIRECTIVES NORMES ET ESTCONFORMEAUX CONFORMS TO THE FOLLOWING DIRECTIVESAN D STANDARDS Directives 89/336/CEECEM Directives 89/336/EEC EMC 73/23/CEEBasse Tension 73/23/EEC Low voltage
Normes t E C 0 ' l - 1 d . 9 9 1 E 1 6 Standards EN50082-1 Ed.1992
E N5 5 0 1 1 l a s sB E d .1 9 9 1 C . E N6 1 0 0 0 - 3 - 2 E d . 1 9 9 5 EN61000-3-3 1998 Ed. DECEMBRE 2OOO DECEMBER 2OOO
FORM 98 Rev4 01
Thismanualis a guide tor the useof the ABG Yef BloodCounter Automated
and for Data thismanual been in verified validated isbelieved beadequate theintended of the to use has and AutomatedBlood Counter.lttheAutomatedBlood Counterorthe procedures usedfor the purpose are validity suitability in confirmationtheir of and should obtained be beyond capabilities the specified thismanual, doesnotguarantee results assumes obligation no or fromABXDiagnostics; ABX and otherwise Diagnostics nor liability. publicationnota license operate This is under a recommendation to infringe upon product any to patents. or process
HAZARDS POTENTIAL features guard operator frominjury, instrufhe Automated safety to the the Blood Counterincludes several frominaccuracies. alertthe operator potentially To hazardous mentfromdamage of and the test results whicharedescribed is wherever necessary headings below provided conditions, of theboldcaptioned one throughout text. this
VUARNING I
Flags a procedure that if not followed properly,can prove to be extremely or hazardousto either the operatoror the environment both.
; CAUTION
I Emphasizesoperating procedures that must be followed to avoid possible to | Oamage or destructionof the instrument.
IMPORTANT
Emphasizes operating proceduresthat must be followed to avoid erroneousresults.
operational function. during aftera specific or
Tableof contents
Section1: lntroduction
Section2 : lnstallation
Section3 : Specifications
Section4 : Technology
Section5 : Description Section6 : Startupand sample run
Section7 : Calibration
Section 8 : Instrumentconfiguration
and troubleshooting Section9 : Maintenance
\_./-
1. INTRODUCTION
CONTENT
-)
1. INTRODUCTION
1.1.INTENDED E US (Microprocessor controlled) is Veterinary)a fullyautomated Blood Counter TheABG Vet(Animal specimens. of blood diagnostic testing whole forthein vitro used hematology analyzer hematology according itsinternal to system as TheABG Vetis available a 8 or 16parameters reagents: setup, anduseof specific ABC Vet 8p: - WBC,RBC,PLT, - HGB,HCT, - MCV, MCHC, MCH, - RBCandPLTdistribution curves. ABC Vet 16p: - WBC,LYM7., MON#, MON%, GRA%, GRA#, LYM#, - RBC,HGB,HCT, RDW, PLT, MPV, MCH,MCHC, MCV, RBC,PLT curves:WBC, and3 distribution fromEDTA). from12 pl of wholeblood(taken
IMPORTANT
- TheABG VetBP in instrumentis available "bottle" and "pack" instrument Upes. - The ABC Vet 16P(LMGE) in is instrument available "pack" instrument type only.
WBC : RBC HGB HCT: MCV MCH MCHC RDW: PLT: M P V: LYM% LYM#: MON% MON#: GRA% GRA#:
cellcount White blood cellcount Redblood Hemoglobin Hematocrit Mean cellvolume hemoglobin Mean corpuscular concentration hemoglobin Mean corpuscular width Reddistribution Platelet count plateletvolume Mean percent Lymphocyte number Lymphocyte percent Monocyte number Monocyte percent Granulocyte number Granulocyte
of per Thisrateincludes timefromintroduction the is42 Therateof determinations samples hour. is The the and and analysis displaying printing results. system to blood sample sample thewhole printer recording test for all anda graphic system dilution with totally automated, an internal printouts. flags results including andgraphic
1. INTRODUGTION
1.2.PRESENTATION in Theinstrument, whichis small size,has7 mainparts: 1 - Theelectrical supply. 2 - Theelectronic board. pneumatics. 3 - Thedilution panel including keyboard a LCDscreen. and 4 - A control a - A reagent 5 compartment. and of curves. out 6 - A printer prints theresults the plotting thedistribution that - A smartcardreader select bloodspecies, runcontrole results. the to bloodandto record 7 to (Diag1.1). at together onepanel, thefrontof thesystem on Allthecontrols grouped are
Diag.l-01
1.3. NOTES Your retains rightto makechanges the instrument thisdocument. to and ABX Diagnosfics the provide latest office revision thisdocument. the of local ABX Diagnosfibsrepresentative will prior or in without may Nopartof thisdocument becopied reproduced anyformor by anymeans written consent ABX Diagnosfics. of
qq
2. INSTALLATION
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2. INSTALLATION
2.1. INSPECTION it. sending We, nevertheless, out is A thorough inspection carried on tne ABC Vet before to any to as the recommend checking totalsystem soonas it is received report anomalies the in procedures be mustimperatively followed the ordergivenbelow. The carrier. installation 2.2.UNPACKING protective in foambefore beingplaced a cardboard in is The instrument enveloped a special, Remove cardboard containing box the the fourangles theboxto unpack system. of box.Cutthe (seeDiag.1). kit tne ABC Vet installalion fromits location
Diag.2-01 type according the instrument : to kits Twodifferent installation areavailable - A ABC Vet "bollle" kit installation - A ABC Vet "pack" kit installation : specifications printers available to according thecountry Twodifferent are - Printer : (220V,50/60H2) model European - Printer model (110V, : 50/60H2) US XAA398A XAA399A
2. INSTALLATION
2.3.PACKAGE CONTENT - TheABC Vetboxes parts : the contain following ABC Vet Printer (english) manual User's "Dog"Smart card "Cat"Smart card "Horse" Smart card
"BloodControl" Smaftcard
8P
16P
R A B0 1 5A 20520 12 20520 13 20520 14 205 20 32 D A C0 1 1A D A C0 1 2A
R A B0 1 5A 20520 63 20520 64 20520 65 20520 73 D A C0 1 1A D A C0 1 2A
(european) Power cable or (US) cable Table 2-01 Power
- TheABC Vet "bollle" kit : installation includes DESIGNATION ReagentstrawL=270mm needle Sampling DiluenUwaste connector male screws Connector Cristaltube 3x6 O ring 6x1.5 1 Oring ,4x1,25 2 Rubber stopper holes
kit 2-02 Commoni nstallation Table
PART NUMBER XEAOlTA GBC069A EAC OO9 A EAC OO7 A E A E0 1 1A FAA036 A FAA053 A
F B LO O 1 A
OTY 3
1
2
4
1
2 3
I
I
X E A3 1 2A
- TheABC Vet "pack" kit : installation includes DESIGNATION

needle Sampling
PARTNUMBER GBC069A FAA036 A FAA053 A X E A3 1 2A
QTY 1
Oring6x1.5 O ring1,4 x 1,25 Table 2-03 kit installation Common
2 2
1
qq
2. INSTALLATION
- The common : kit installation (XEA312A) includes DESIGNATION Fuse1A,220V5P.0 Bentwrench 2.0 Bentwrench 2.5 Bent wrench 1.5 Allen 2.5 key Tox keyT10 Tygon tube1.52 Tygon lube2.29 T210-6 Fitting 1.6mm T PAHTNUMEER
DAR O4O A MAB OOlA
ATY
2 1
I I
MAB OO2 A MAB OO3 A MAB 069 A

MAB O9O A
1 1
EAE OO7 A EAE OO9 A EAB032A

F A A0 1 7 A
2 2
I
o ring30.8x3.6
.5 O ring15x1 KM Grease 1011 ABC Vetcover Table 2-04 ABC Vetcoverkey
1 1 1 1 1
FAA029 A X E A0 1 9A F B H0 1 5A
FAJ OO4 A
2.4.WORKING CONDITIONS Please notethatABC table workstation. or andlevel on ABC Vel should placed a clean be (66lbs).Avoidexposure sunlito 30 weighapproximately kilograms and reagents Vet, prinler must leftbehind the 20 be of ght.Proper ventilation requires a space at least cm (8 inches) that apparatus. check Electromaqnetic 2.4.1. environment levelof electromagnetic lessthanthe required to The ABC Vet hasbeendesigned produce The interferences to in in interferencesorder operate conformity itsdestination. electromagnetic to in operation otherinstruments of levelallowing correct the to by the ABC Vet arelimited a with r conformity thei destination. is in of check theinstrument notplaced theproximiy electromagnetic that Incaseof problems, (radars, scanner, etc...). X-rays, waveemissions fields, short or protection 2.4.2.Environment in mustbe collected a plantspecialized by and Whenold the instruments theiraccessories according thelegislation. to of and elimination recycling thiskindof equipment 2.5.GROUNDING (earth) plugis correctly to connected grounding required. that is Check thewallground Proper lf thereis no ground stake. thenusea ground grounding installation. electricity the laboratory mustbe applied. norms Current electricity
2. INSTALLATION
2.6.HUMIDITY TEMPERATURE AND CONDITIONS 18 ABC Vet 8p can function between to 32'C (65 to 90'F),ABC Vet I6p can function humidity, meaning than85%withnocondenless 20 between to 30'C(68to 86'F),withrelative (50'F), instrument less lf the to sation. it is kept a temperature than1O'C at should allowed sit be roomtemperature before use. for an hourat thecorrect 2.7.VISUAL CHECKS 2.7.1. Mechanical check kit, into in the as on Using keycontained theinstallation turnthelocker shown theDiag.2.2 order protection door. to open pneumatic the
Diag.2-02 f andremove cover: pullit backward liftit upto the the Unscrew 5 cover ixation screws and the (Diag. rear theinstrument 3). of
Diag.2-03
qcl
2. INSTALLATION
to locking (A) as far as possible the leftand placethe sample clip Pushthe plastic carriage in here (B) to side, as needle carriage as farforward possible theright-hand as shown Diag.4, position. (C) sureit is in itsupper that below. Check the needle is notbentandmake .J
Diag.2-04
in Each be below. chamber should in its as of Check position thechambers shown Diag.S the is firmly the RBCchamber. block attached to proper position itsclips theelectrode with and
Diag.2-05 block 1 - Electrode 3 - RBCchamber 2 - Clip 4 - Mixing chamber
2. INSTALLATION
protection chamber cover<1>.Check thatthe of Unscrew slightly 2 screws theWBC/Hgb the firmlyto the chamber in blockis attached is chamber fixedproperly its clipsandthe electrode (Diag.6).
Diag.2-06 2.7.2. Connection check circuit in 7). boardaresecurely place(Diag. on Check theconnectors the printed that
+n
Diag.2-07
2. INSTALLATION
pressing theholder (Diag. lock 8) on on f Remove fuseholderromitslocation therearpanel the 220 Slow-Blow' be : they the andcheck fusecharacteristics should 1 Ampere, Volts
*HY|H'@-9
Diag.2-08
cover. coverandthe instrument chamber Re-install HGB^ruBC the CONNECTIONS 2.8.REAGENT : packages available are Tworeagent - Bottles containers (ABCVetSP only). and - Reagent pack. . 2.8.1Bottleconnections in 9. as compartment shown theDiag. the inside reagent are reagents placed Lyse cleaning and and bottle thebluetubeto thecleaner Connect stoppers. and Install reagent straws thebottle the cover' the Close compartment thewhite tubeto thelysebottle.
Diag.2-9
2. INSTALLATION
: CAUTION I The diluent containerwill be locatedon the bench at the same level than the
instrument.
in kit input output included theinstallation attheliquid located at Install male and the connectors (Diag. 10). rear thebottom theinstrument panel of (see above) tube using diluent the straw a 3x6cristal and Connect diluent the container CAUTION (1 metermaximum) the diluent inputlocated the bottom the instrument panel. rear at of on using cristaltubex 6 onthewaste the 3 output, place waste and the Connect waste the container level bench). container below instrument (underthe the
DILUANT DECHETS DILUENT I4IASIES
Diag.2-10
procedures wastedisposal,Neverconnect for Alwaysfollow the recommanded drain pipes,Foreachwasteconthe instrumentwastesdirectlyto the laboratory procedureas describedin section2.9. tainer,follow the neutralization packconnection 2.8.2. Reagent in TheReagent Packincludes entire of reagents a "Pack" is ableto receive waste the set and the pockets by liquids. Three contain 3 reagents the diluent, cleaner lyseandareclosed and soft pocket empty located thebottom thepack. fourth at The is and means thevalveconnectors of of liquids. is intended receiving for waste plateinorder remove protection plugs to the located the at Push downwards thepacksupport on packlocation (Diag. 11). bottom the of
Diag.2-11 12. into of as Install pack on the directly thecompartmenttheinstrument shown theDiag. Push the to connectors. thepackdownin order plugcorrectly packon themale
qq
2. INSTALLATION
ffi
Diag.2-12
valvein orderto on (see Thefreemaleconnector Diag.13)mustbe plugged the packupper liquids. receive waste the
Diag.2-13
CAUTION:
not In order to avoid leak problemsit is recommended to unplug severaltimes the same reagentpack,
2. INSTALLATION
PRECAUTIONS HANDLING 2.9.REAGENT ANDWASTE (15'Cto 25"C;59'F to77'F). Operating at Reagents haveto be stored roomtemperature 2.6. contains cyanides have be handled and to conditions givenin section Lysereagent are precautions regulations. Always followthe recommended according the localor national to (seesection for the reagent specifications). 3.3 require,waste liquids can be neutralized lf your local or nationalorEanizations beforebeing discarded. - Wastefiquidsof lhe ABCVet canbe neutralized usingthe followingprocedure: then wasteliquids,add 50mLof SodiumHydroxidesolution 200g/L, For 20L of solusolution 12'Gl daily preparedfrom the commercialized 250mLof bleach tion (48'Cl). - Gyanides add from the lysing reagent(ABC Vet8P):for 20L of lysing reagent, sotu50mLof SodiumHydroxide2}lglL,then 100mLof AmmoniumPersulfate or tion 500g/Ldaily prepared, add 50mLof SodiumHydroxide2ililglL,thenadd solution30%. 500mL SodiumHypochloride of
WARNING!
NOTE
with the pack,this one collects the wastegenerated all nstruments equipped with compliance the latest environmental inoto facilitate
2.1 ELECTRICAL O. CONNECTIONS in electrical to supply using power the cableincluded TheABC Vet is connected the laboratory 2.3). Connect power the on cable thepluglocated therearleftto installation (see kit section the (Diag. 1a). hand sideofthedevice plug. Theinstrument be operated anyother the can at under power Two1Afusesarelocated, (from to 60Hz) without modification. (from to 240V) frequency 50 100 or voltage
wiF
-o^o
Diag.2-14
SWITCH 1 - ON/OFF HOLDER 2 - FUSE PLUG SUPPLY 3 - MAIN
4 - RS232 OUTPUT 5 - PRINTER OUTPUT
qq
2. INSTALLATION
POINTS 2.11. GENERAL .1 lt device. responds the601 to 1 TheABG Vel is classified a class , typeB electro-medical as Worksecurity reliability general and Commission. Electrotechnical normof the International under following the conditions only: by characteristics guaranteed ABX Diagnosfics are - services repairs provided a AB<x Diagnosfics authorized technician by and are - theelectricalsupply laboratory regulations or follows national international the of the - thesystem operated instructions. the is under following next located the rearsideof the instrument, to the at : of SYMBOLSSignificationthesymbols plug: main
(ground) Earth
documents read Warning: enclosed
OFF(supply)
ON(supply)
current Alternating of to conforms the requirements the CEM89/336/EEC This product 73i and by directive thelowvoltage modifyed the93i68/EEC directive, ?31EEC directive.
providing particular of Equipement degree a TYPEB EQUIPEMENT. particularly regarding : protection electric shock, against - allowable leakage current - reliability the protective of earth connection.
2. INSTALLATION
2.12.PRINTER : CAUTION Do not attempt to connect a printer which is not recolnmendedby a ABX technicianand which is not approvedby the norms CEI Diagnosticsauthorized or 601-1 CEI950.
with Lock The printer connected ABC Vet withthe cabledelivered the instrument. the is to place tightening 2 screws eachendof theconnector the ABC Vet. in the on to by connector in Attach otherendof the cable the printer lockthe printer to and connector placeby the the itself 15). on means the2 clipslocated theconnector (Diag. of
Diag.2-15
NOTE:
See printer documentationfor rnoredetails
2.13. REAGENT PRIMING it no installed,contains reagents. Allthereagents to beprimed have When ABC Vetisfirst the located therearpanel. When the now. TurnON instrument pressing ONiOFF by the switch on : instrument on,thedisplay shows turns
panel fromredto green thedisplay turns the After minutes, LEDof thefront the and shows three following:
qq
2- INSTALLATION
(seesection is withthemanual startup cycle whentheinstrument setup Thismessage appears running startup cycle.Press anykey,the main a cyclebefore 8.3.3) prevent analysis any to is : menu displayed
Bottlesand containers set 2.13.1. line cycle clear reagent of air bubbles. to the andcarryouta PRIME Install reagent bottle the is a of be Thisprocedure should donewhenever newbottle reagent installed. The ENTER. and to move cursor thefunction SERVICE press the From MAIN MENU, the f?l : menuis displayed service
''
ENTER. PRIME The menuis and f Move cursor function pnfftf REAGENTS press the to |3l \-_J : displayed
nextto the required or the Select either function Orir" ALLREAGENTS move cursor the O ENTER. reagent press and is : menu displayed while following the Thepriming cycle starts
NOTE
for inspect reagent lines visually andpumps air bubbles. Before samples, analyzing ABX Diagnostics representative Callthe priming airbubbles stillpresent. if are Repeat priming air doesnoteliminate bubbles. service departmentif
: CAUTION
foaming This causesexcessive Neverinitiatetwo lyse prime cycles back-to-baek. in the waste chamber.Run a blank cycle betweer ?ch lyse prime cycle.
2. INSTALLATION
pack 2.13.2. Reagent move cursor thefunction the to From MAINMENU, the Q : menuis displayed service ENTER. The SenVlCE andpress
givenby the LCDin order install pack. PACK follow instructions the to the CHANGE and
Move cursor C the ro
PACK key. REAGENT andpress ENTER Select function the the O
will is cycle beautomatically a carried andthefollowing out Once newPACK installed priming the menu displayed.
NOTE
inspect visually reagent linesandpumps air bubbles. for Before analyzing samples, priming (REAGENT PACK menu, function : eRfME)if airbubbles still are Repeat @ present. if does servicedepartment priming CalltheABX Diagnosticsrepresentative noteliminate bubbles. air
left menu, function Oisplays number analysis PACK the the of cycles to From REAGENT the O runwiththesamepack. cycleat anytimeusingthe selection tt*'"e) It is alsopossible runa priming to @ PACK menu. REAGENT of the
: CAUTION
not I lt is recommended to removethe pack severaltimes beforethe reagentsare I toully used in orderto avoid leak problems.
qq
3. SPECIFICATIONS
.......... PERFORMANCE 3.1. SPECTFTCATIONS REAGENTSPECTFTCATTONS 3.3. n 3 . 3 . . D i l u e AtB CV e t8 P : A B CD 1 1 . . . . . . . . . . . . . 1 ......... 3.3.2. Lyse ABCVet8P : ABCLYSE . Ve C 3 . 3 . 3C l e a n e r A B O t8 P : A B C L E A N. . . . . . . . . . . . . . . packABC 8P Vet 3.3.4. Reagent packABC 16P Vet Reagent 3.3.5. :t 3 . 3 . 5 . 1 i l u e nA B CD I L1 M G . . . . . . . . . . . D LMGE 3.3.5.2. Lyse:ABCLYSE .. :A C 3 . 3 . 5 . 3 .l e a n e r B C L E A N . . . . . . . . . . . . . . C M 3.3.6. INOCLAIR 3.4.1. 1eanin9............. C Blood 3.4.2. specimens Known interfering substances 3.4.3.
2 .............. ............... I . . . . . . . . . . 8. . . . ................ 8 ...................... I ............ I .......... I ................... 9 ....... 9 . . . . . . . . . . 9. . . . . . . . . . . . . .1 . . .0 . . . . .1 0 . .....10 ......10
qq
3. SPECIFICATIONS
SPECIFICATIONS 3.1.PERFORMANCE no for operations aspirating and blood counts, requires manual ABC Vet performs automated given print-out, computer The transfer. parameters and measuring, calculations, blood, dilution, areas follow: setup according the internat to ABC Vet 8p: - WBC,RBC,PLT, - HGB, HCT, - MCV, MCH, MCHC, - RBCandPLT curves. distribution ABC Vet 16p: - WBC,LYM%, MON#, GRA%, GRA#, MON%, LYM#, . RBC, RDW, PLT, MPV, MCH, MCHC, MCV, HGB,HCT, PLT WBC,RBC, curves: and3 distribution - Dimensions inches) 440 (Diag.3.1) Height:approximatelymm(16,5 :- Width: approximately mm(14,2 inches) 360 - Depth approximately mm(12,6 inches) 330 :
-o
SSSeo
Diag.3-01
- Option smart : card
* Reader: GCl400 . Calibration :GFM2K card . Memory : card : - MCOS24K(capacity 60 CBCs) - MPCOS (capacily CBCs) :78 32K
3. SPECIFICATIONS
- Weight : - Power : supply - LCDscreen : - Power : Consumption - Conditions use: ABC Vet 8p for ABCVet16p - Hemoglobin: - Sizeof apertures : - Finaldilutions : - Throughput : - Veterinary smartcards: NEFEHENCEPN / 16P NEFERENCE'1PN 8P * approximately (31 14Kgs lbs). * 100Vac 240Vac 10/",50 Hzto 60 Hz to + * 2 lines 40 characters, of backlighted * Maximum 150VA(-30"/", 10%) : + * In use: 110VA(-30/", 10%) + . Stand-by mode:35 VA(-30/",+10%) . 18 - 32'C (65- 90"F)roomtemperature .20 - 30"C(68to 86'F)roomtemperature * 85/omax(rel.humidity, condensation) no . HGB/WBC LED chamber, 555nm. * WBC= 80 pm . RBC/PLT 50 = Um * WBC= approximately 1/300 . RBC/ PLT= approximately 1/20,000 . 45 sampleshour / approximately
ESPECES SPECIES /
Chien Dog " I Chat/ Cat * Cheval Horse I
205 20 63 205 20 64 205 20 65 205 20 66 205 20 67 205 20 68 205 20 69 205 20 70 205 20 71 205 20 72 * 205 20 73
20520 12 205 20 13 20520 14 40520 16 20520 17 205 20 36 205 20 37 205 20 38 205 20 39 245'204:0 205 20 32
Rat/ Hat
Souris Mouse I Vacfp I eow
Lapin I Rabbit
Co0hon Pig / Mouton Sheep I
Sirye/ Mohkey
I Table 3-01 Controle Control
* Those with are smart cards delivered theinstrument
3. SPECIFICATIONS
- Capacity internal memory: of - Volume whole : sample blood of - Reagent : consumption CYCLE * Lastsample only . 12pl * Software version 2'21 V
DILUENT
27ml
LYsF
1ml
CLEANER
X
DIL.BLEAGH
X X X
Sartup Stand-By
Analysiscycle
x
20ml
x
0.6m1 10;7ml
X
9ml 1.3m1
Prime allreagents
Primediluent
30ml
30ml 6.5m1
X
5.3rnl
X X
x
X
Primelyse
Primecleaner Auto clean cycle cleaning Concentrated HGB blank cycle Backflushcycle Table 3-02
10,7m1
X
x
X
5.3m1 9ml
X
17,5m1 17,5m| 6,0m1

X
x
X
6ml
X X
1.3rnl
X
X
X
- Option: Reagent pack - Measuring principles :
* Total : capacity 4,2 litres * WBC'RBC.PLT = lmpedance change - HematocritNumeric = integration 'HGB (ABCyefBP)=Cyanmethemoglobin (550nm) method ' HGB(ABCyef16P) Chromogenous = cyanide{ree complex (550nm) method
3, SPECIFICATIONS
- Reproducibility whole withoul from blood sample samplings onefresh : (based 20consecutive on alarm) PARAMETERS WBC RBC HGB HCT MCV
PLT CVo/" <2,5
NORMAL RANGE
4 to 12 x '103/mm3
<2
< 1.7 <2
< l
5 to 11x 106/rnmo 8 to 20 g/dl

27 la 57 o/" 37 to 77 ll
<5
100to ts00 103/mmg x 13to 24 pg 26 to 39 gldl (%) Undefired 13to65% 0to7 "/" 25 to 78 "/" (fl) Undefined
MCH MCHC RDW Lymphocytes(o/o) (%) Monocytes Granulocyte$f1o) Table 3-03

MPV
- Linearity Linearity low was testedusinga commercially available rangeand full range : and according themanufacturer's to Thekitswere analyzed datawascomputed linearity kits. test Each value. and usedas the reference six instructions. kit included levels onelevelwas Each wereas follows : Theresults thisstudy of level was runfourtimes. IMPORTANT I Linearitylimitsare differentfrom a specieto another
PARAMETERS
WBC(x103 cells/pl)
LNEARTTY RANGE 0,5to80 0.2to 7.5 10to 1000 2.5to 23

11 to 55 .6
LMMS (whichever greater) is +l- 0.2or +l- 3o/o (whichever greater) is ii- 0,05or +l- Zoh (whichevergreater) +l 10or +l- 60/o is (whicbveris greater) +/-0,3or +l- 2o/o (whichevergreater) +l-2 or +l-3o/o is
RBC(x106 cells/pL)
PLT(xl03cells/pl) FIGB.{gldL)
HCr(%)
Table 3-04
56to62.4
(whichevergreater) +/-5 or +l- SYo is
3. SPECIFICATIONS
- CarryoverCarry-over tested analyzing with of samples highconcentrationsWBC's, by was : followed three cycles. by background was Each sample runintriplicate, RBC'5, HGBandPLT's. : formula the is using following f he"h carryover calculated - Background3 Background'1 Carryover X 100 Sample3 Background3 WBC Level o/"Carry-over (actual) Table 3-05
CVo/o claim
RBC
7.58
HGB 23.4 0.0

<0.5o/o
PLT
988
63.0 0.3
<0.5o/"
0.00
<0.5%
0.0
<0.5/.
- Accuracy Approximately patient were analyzed the ABC Vet and a on specimens 200 : The locations theUnited in States. different at analyzer three reference commercially available the following table summarizes data:
S I T E1
PARAMETER '103/mm3) WBC (x RBC (x 106/mm3) N R N
SITE 2
R N
SITE 3
R
198
198
0.992 0.995
0.994
209 212 212 212 212 212 212 212 201 204 110
104
0.997 0.995
0.998 0.994 0.987
203 204 204 204 204 204 204 204 198 203 119 119
'119
0.995 0.990 0.985 0.982 0.980 0.926

4.471
H G B( s / d L ) H C T( % )
MCV (fL)
1BB
198 198
0.980 O.9BB 0.969 0.311 0.950 0.994 0.639 0.975 0.552 0.969
M c H( p s )
McHC (g/dL) R D W( % )
PLT (x 103/mm3) MPV (fL)
188
188
0.962 0.654
0.944 0.981
198 169
191
0.895 0.926 0.863 0.970

0.461 0.968
0.709
0.991
(%) Lymphocytes M o n o n u c l e a( % ) rs (%) Granulocytes

Table3.6
9B
98
0.787 0.990
9B
105
Reference Analyzer
B a k e rS y s t e m 9000
C o u l t e S P l u sl V r
C o u l t eJ T r
3. SPECIFICATIONS
3.2.PRINTER - Size(seeDiag.3.2.) . Height approximately mm = 132 : . width = approximately mm 275 . Length approximately = 366
Diag.3-02 - Weight: 4 approximatelykilos 230VACI 10/oor 110 VAC+ 10/" 50/60hz tl{z
- Power : supply
- Powerconsumption: W 30 - Paper(Zfold): - Ribbon : - Printing mode: - Printing speed: - Butfer : - Number columns : of Width: 102i 254mm nylon black g Matrix serialimpact needles per for 264Characters second 12cpi 4 Koapprox. 80for 1Ocpi.
3. SPECIFICATIONS
SPECIFICATIONS 3.3.REAGENT ABX Diagnostics reagents must used be high-quality correctly, lnorder theinstrument operate for to reagents atemperature at date expiration andstorethe Respect provides reagents. allthenecessary 15 between to 25"C(59to 77"F).
IMPORTANT
in is instrument not available "bottle"instrument TheABCVet16P(LMGE) type.
ABC Vef8P : ABCDIL 3.3.1.Diluent in the involved counting identifying blood and for is Function: Thediluent necessary theprocess cells. Azide <0.1"h. Sodium phosphate containing buffer : Composition Saline solution. aqueous : and DescriptionLimpid odorless point About PH 100oC, : neutral. : properties Boiling : Physico-chemical gloves whenhandling the Use skinandeyecontact. laboratory : PrecautionsAvoid Handling irritation result. can a is quantity reagent ingested mucous of lf a large reagents. flushi rinse EmergencyFirst aid : lf the eyesor skin come into contactwith the reagent, vomiting. immediately, induce and quantity ingested, water drink is lf withwater. a large abundantly LvseABCVef8P: ABGLYSE 3.3.2. salt potassium a ammonium and at cyanide 0.06%, quarternary contains Function: Thereagent < azide 0.1"/o. phosphate sodium containing buffer a saline limpid. solution, DescriptionAqueous : point approximately 100"C. : basic. PH : properties Boiling : Physico-chemical gloves Wearlaboratory witheyes,skinand clothing. :Avoidcontact HandlingPrecautions The if can may product. product beharmful ingested. product beabsorbed The whenhandling the wound, inhalation. or through open an lf witn the orskincomeintocontactwith reagent,ilush water. FirstAid: lf theeyes Emergency quantity ingested, water is drink a large freshairimmediately.lf is breathe thereagent inhaled, doctor. center, contact or Call vomiting. localanti-poison immediately, induce and ABC Vef8P : ABGCLEAN 3.3.3.Cleaner Maybe harmful. agent. Function: Washing proteolitic derivatives. and enzyme tensioactive containing buffer isotonic : GompositionStabilized whichis colourless. solution Description: Aqueous point: around 00'C. PH : basic. 1 properties: Boiling Physico-chemical witheyes,skinandclothing :Avoidcontact HandlingPrecautions lf withwater. with comesintocontact theeyes,rinse FirstAid : Incasetheproduct Emergency a is call the product ingested, immediatelydoctor.
3. SPECIFICATIONS
packABC Vef8P 3.3.4.Reaqent above. of Seethespecificationsreagents packABC Vet16P Reaqent 3.3.5. pack LMGEcontains following reagents the : TheABC reagent ABG 3.3.5.1.Diluent: DILLMG for involved counting differentiating in the Function Thisdiluent necessary theprocess is and : blood cells. which : solution contains organic an buffer, antiseptic an saline CompositionStabilized < Azide 0.1%. andSodium solution. : aqueous DescriptionLimpid odorless and point About properties Boiling : : 100'C,PH: neutral. Physico-chemical gloves whenhandling the : Use HandlingPrecautions Avoidskinandeyecontact. laboratory quantity reagent ingested mucous is irritation result. of a can reagents. a large lf withthe reagent, flush/ rinse Emergency First aid : lf the eyesor skincomeintocontact quantity ingested, water is vomiting. drink immediately, induce water. a large lf and with abundantly LMGE 3.3.5.2. Lyse: ABCLYSE hemoglobin is cells, determine and Function: The reagent usedto lyseblood concentration perform whiteblood differentiation. cell free. contains tensioactives is potassium and cyanide : CompositionThereagent DescriptionAqueous : solution. point approximately properties Boiling : : 100'C.PH:basic Physico-chemical gloves witheyes,skinandclothing. Wearlaboratory :Avoidcontact HandlingPrecautions product. product be harmful ingested. product beabsorbed may if The The can whenhandling the wound, inhalation. or through open an lf with flushwithwater. Emergency FirstAid : lf theeyesor skincomeintocontact thereagent, quantity ingested, water freshairimmediately.lf a large is drink is breathe thereagent inhaled, vomiting. local Call anti-poison center, contact or immediately, induce doctor. and ABCCLEAN 3.3.5.3. Cleaner: Maybe harmful. Function: Washing agent. proteolitic containing derivatives. : isotonic buffer enzyme tensioactive and GompositionStabilized whichis colourless. Description :Aqueous solution point around properties Boiling : 100'C.PH: basic. : Physico-chemical witheyes,skinandclothing : contact HandlingPrecautions Avoid lf into with withwater. comes contact theeyes,rinse Emergency FirstAid : Incasetheproduct product ingested, immediatelydoctor. a the is call
qq
3. SPECIFICATIONS
MINOCLAIR 3.3.6. procedure. Diluted bleach solution also can cleaning MINOCLAIR usedin theconcentrated is precautions emergency aidrecommendations. first and handling the be used.Respect same Maybe harmful Function:Cleaning agent (0,26%) (4%), Hydroxide Hypochlorite Sodium :Sodium Composition smell chloride. of and colorless hasa typical :Aqueous solution, Description + point: 100'C.PH : 12,9 0,5at 20oC' properties Boiling : Physico-chemical with Rinse lhoroughly water skin, clothing. and with precautions :Avoid contact eyes, Handling gloves whenhandling whennotinuse.Wearlaboratory closed Keep container the afterhandling. if or, maybe harmful ingested inhaled. The theproduct. product with into with thoroughly comes contact skin,rinse FirstAid : In casetheproduct Emergency for and withwater 15 minutes callfor a medical rinse witheyes, into water. it comes contact lf immediately. and Drink lotof water calla doctor a vomiting. lf do check. ingested, notinduce 3.4.LIM|TS Cleaning 3.4.1. procedures listed. mainteThe are and shutdown, maintenance In section specific start-up, 9, use of for are identified mandatory the proper andoperation the ABC Vel. nanceprocedures IN MAY PROCEDURES RESULT RECOMMENDED OFTHESE FAILURE EXECUTEANY TO OF RELIABILITY THESYSTEM. DECREASED Bloodspecimens 3.4.2. of (including flagged results results outside thenormal or test Verificationanyabnormal result of procedures for laboratory standard methods other or reference using range) be should performed blood list limitations automated of below known sections verification results.The of theconclusive of which theprinciple impedance. use cellcounters Knowninterferinq substances 3.4.3. : WBCWhiteBlood Cells(Leukocytes) sample. will dilution theblood of limits thesystem require of the WBCresults exceed linearity that value. the assay will to sample help obtain correct Re-assaying diluted the Blood Cell) in red nucleated bloodcells willbe counted the WBC(White NRBC- lmmature on blood parameter. differentialwhite cellcount performed thestained However, manual the presence NRBC's' of film blood - willrevealthe for whitebloodcellcount,the WBCassayvaluemustbe corrected the Following manual the for the utilized correcting WBCparameter, presence nucleated bloodcells.Theformula red of is red whennucleated bloodcellsarepresent, : WBCX 100 counted CORRECTWBC: WBC) 100+ (#of NRBC/100
3. SPECIFICATIONS
rare in maynot the sample UnlysedRedCells- Inparticularly instances, erythrocytes theblood will WBCcount. lyse. erythrocytes cause falsely a elevated completely Non-lysed patients giveelevated in myeloma may Multiple myeloma Theprecipitationproteins multiple of WBCcounts. contain cellstroma red whichmayelevate white counts. Hemolysis Hemolyzed specimens cell mayresult thisdisease in low LeukemiaA spurious WBCcount state because thepossible of leading some to increased fragility theleukocytes of destruction these of cells during counting. of and drugsmay increase fragility the the Chemotherapy Cytotoxic immunosuppressive low leukocytes which maycause WBCcounts. levels cryoglobulin maybeassociated myeloma, of that with carcinoma, CryoglobulinsIncreased lymphoproliferative metastic leukemia, macroglobulinemia, disorders, tumors, autoimmune pregnancy, phenomena, aneurism, infections, idiopathic disease, thromboembolic disorders, value. specimen be counts theHGB and The can the diabetes, canelevate WBC,RBCor PLT etc immediately a manual WBC,RBC, PLT can warmed to 37'C andreanalyzed or or count be up performed. : RBCRedBloodCells(Erythrocytes) alltheformed elements theblood erythrocytes, in : leukocytes, Theredblood contains celldilution platelets notcounted (red of cells), During counting theerythrocytes blood the are andplatelets. threshold. the since theirsizefallsbelow minimum (White hand, included theRBCcount. in However, since Leukocytes cells), theother on are blood of ratiobetween blood red cellsandwhiteblood cellsis so extreme. influence the the thenormal WBConthe RBCis negligible. where WBCis extremely high, RBCcountmaybecorrected, the the HighWBCs- ln rarecases low especiallythe RBCcountis extremely if RBCcount.Bloodsamples Agglutinatedred blood cells - May causea falselydecreased red MCHand by abnormal containing agglutinated bloodcellsmaybe identified observing the of film. MCHC values, wellas by examinationthestained blood as which may in disease lower Coldagglutinins lgMimmunoglobulins areelevated coldagglutinin MCV. RBCandPLTcounts increase and HGB(Hemoglobin): factors of and/or therapeutic Turbidityof the blood sample- Dueto anynumber physiologic To HGBresults. obtain hemoglobin results accurate whenincreased mayproduce falsely elevated determine cause theturbidity follow appropriate the of and sample occurs, the turbidity theblood of method below: WBCwill causeexcessive scatter. these light ln WBC :An extremely elevated 1. Elevated (manual) methods.The and reference diluted sample shouldbe centrifuged, the casesuse f luidmeasured a spectrophotometer. on supernatant lipids theblood in willgive plasma "milky" 2. Elevated lipids : Elevated sample the a appearance. (as hyperproteinemia in gammapaties) and This condition occurwith hyperlipidemia, can reference hemoglobin determinations be achieved using can hyperbilirubinemia. Accurate by (manual) blank. methods a plasma and
qcl
3. SPECIFICATIONS
to cellsareresistant lysing. the may lncreased turbidity alsobe seenin casewhere redblood the Hgb by but elevated result, maybe detected observing a Thiscondition cause falsely will edgeof the WBC baseline the leading on values, the increased and MCH,MCHC abnormal to will of the results cause results the MCHandMCHC be hemoglobin histogram. Erroneous erroneous well. as HGB bloods mayproduce falsely a elevated of Fetalbloods- The mixing fetalandmaternal value. HCT(Hematocrit) HCT Red cells erroneous andMCVvalues. blood Redbloodcellsagglutination Mayproduce MCHandMCHC values, wellasby as the by may agglutination bedetected observing abnormal methods be required obtain may to manual cases, film blood ln such examinationthestained of HCT anaccurate value. Volume) MCV(MeanCorpuscular MCVvalue. Redblood cellagglutina an Redbloodcellagglutination Mayproduce erroneous MCHandMCHC values, wellasbyexamination as the by tionmaybedetected observing abnormal methods be required obtain accurate may to an manual film.Insuchcases, of thestained blood MCVvalue. Excessivenumbersof large plateletsand/orthe presenceof an excessivelyhigh WBC ln careful of determinationtheMCVvalue. suchcases, with count- Mayinterfere theaccurate the film blood mayreveal error' examinationthestained of : Hemoglobin) MCH(Mean Corpuscular The listed and value theRBCcount. limitations fortheHGB and of TheMCHisa function th HGB values. erroneous on RBC have effect theMCHandmaycause will an Concentration) Hemoglobin (Mean MCHC Corpuscular limitations fortheHGBandHCT listed is of TheMCHC a function theHGBandHCTvalues.the values. erroneous and willhaveaneffect theMCHC maycause on Width) RDW(Redblood cell Distribution Theredblood dilution cell of widthis a function the RBCcount. The redblood distribution cell During leucocytes platelets. and in elements theblood: erythrocytes, contains oftheformed all in (Red are cells)platelets notincluded RBCcountsince of thecounting theerythrocytes blood (White leucocytes cells) counted are However, blood threshold. the theirsizefallsbelow minimum the ratiobetween RBCandWBCisso extreme, the Since normal in andincluded theRBCcount. especially low influence theWBCis extremely andthe RBCcountmayneedto be corrected, of low. if the RBCcount extremely is RDWs. Blood RBCcountanderroneous decreased a Agglutinated RBC- Maycause falsely MCHand by abnormal RBC the samples containing agglutinated maybe detected observing film. blood of MCHC values, wellas by examinationthestained as RDWresults to iron, due Nutritionaldeficiencyor blood transfusion- Maycauseelevated RBCdistribution mayalsobepresent frombi-modal High vitamin orfolate conditions. RDWs 812 in blood transfusion.
3. SPECIFICATIONS
PLT(Platelets) Verysmall erythrocytes(microcytes) erythrocytesfragments(schizocytes), WBC and , with fragments Mayinterfere theproper counting platelets cause of PLT and elevated counts. The Agglutinated causing erroneously platelet an low erythrocytes Maytrapplatelets, count. presence agglutinated maybe detected observation abnormal erythrocytes by MCHand of of of MCHC values bycareful examinationthestained blood film. and low Giant plateletsin excessivenumbers- may causean erroneously platelet countsince parameter are not may exceed upperthreshold the platelet the for theselargeplatelets and counted. and drugs of ChemotherapyCytotoxic immunosuppressive mayincrease fragility these the (manual) Reference methods may be necessary to cellswhichmaycauselow PLTcounts. platelet count. obtain accurate an platelet red Hemolysis Hemolyzed contain cellstroma whichmayelevate specimens counts. platelet with may A.C.D. anticoagulated acid-citrate-dextrose contain blood- Blood aggregates platelet which the count. could depress inclusions, as Howell-Jolly such RBCinclusions Erythrocyte bodies, Heinz siderotic bodies, granules, mayproduce spuriously platelet a increased etc count. andbasophilic platelets to poor Platelet due collection techniques platelet satellitosis agglutinationClumped or platelet may of a an caused EDTA by activation immunoglobulins cause decreased countand/or WBCcount. specimen The should recollected sodium be in and elevated citrate anticoagulant ThefinalPLTresult reanalyzed onlythe platelet for count. mustbe corrected the sodium for citrate dilution effect. MPV (MeanPlateletVolume) Giant plateletsthat exceedthe upper threshold of the Plateletparameter- May not be platelets notbe included theinstrument's will these in counted platelets. as Consequently, larger Volume. Platelet calculation Mean of erythrocyticfragments(Schizocytes), Very small erythrocytes(microcytes), and white with counting sizing Platelets. and blood cell fragments- Mayinterfere the proper of MPV The Agglutinated causing erroneous result. presence an erythrocytes MaytrapPlatelets, may MCHandMCHC by of of agglutinated erythrocytes bedetected observation the abnormal values bycareful of film. examination thestained blood and the Chemotherapy Mayalsoaffect sizingof PLTs.
IMPORTANT
in not Volume. Blood collected EDTAwill maintain stable a MeanPlatelet samples in withtimeandtemperature. Platelets swell collected EDTA
'l
c|c|
3. SPECIFICATIONS
(Lymphocyte countabsolutevalue) LYM# red The presence nucleated blood of fromthe WBCcount. countis derived The Lymphocyte with that to parasites, erythrocytes areresistant lysismayinterfere an and certain cells(NRBC), # as to listed Limitations fortheWBCcountpertain the LYM count well. LYM accurate count. percentage) (Lymphocyte LYM% The of percent a function theWBCcountandthe number Lymphocytes. of is Thelymphocyte parasites, erythrocytes are resistant to that and ceftain presence nucleated RBC(NRBC), of pertain for Limitations listed theWBCcount LYM% count. with lysismayinterfere an accurate to the LYM% well. as (mononuclear countabsolute) MON# cell presence large fromthe WBCcount.The of is The mononuclear countabsolute derived cell may number basophils interfere of and lymphocytes, blasts, excessive lymphocytes, atypical number monocytes of withan accurate percentage) MON%(Mononuclear of percentage a function the WBCcountandthe number monocyles. of is The mononuclear number of lymphocytes, and excessive blasts, atypical The presence largelymphocytes, of count. MON% with may basophils interfere an accurate cell GRA#(Granulocyte count absolute) presence of The excessive fromthe WBCcellcount. cell The granulocyte countis derived may promyelocytes, blasts, plasmacells interfere and myelocytes, metamyelocytes, eosinophils, granulocyte count. withan accurate percentage) GRA%(Granulocyte of of percentage a function theWBCcountandthe number thegranulocyis Thegranulocyte promyelocytes, blasts, myelocytes, metamyelocytes, presence eosinophils, of tes.Theexcessive GRA%count. with andplasma cellsmayinterfere an accurate
4. TECHNOLOGY
4 . 1 . 1s a m p r o , a * i n g . . . : : : : : : : : : . . . : . . : . . : . . : : : : . . . . . . . . . . . . . . e 4 . 1 . 2S a m p l i n g e d l r i n s i n g . ne e rinsing 4.1.3. Chamber needle and 4 . 1 . 4F i r sd i l u t i o n . t 4 . 1 . 5S e c o na s p i r a t i o n . d 4.1.6. Second dilution transfer and 4.1.8. Rinsing drain and 4 . 2 M E A S U R E M EP R | N C | P L E S . . . . . . . . . . . . . . . . . NT principles 4.2.1. RBC/ WBC/ Pltdetection principle 4.2.2.Hemoglobin measurement principle 4.2.3. Hematocrit measurement 4,3. DISTRIBUTION CELL STUDY 4.3.1. White blood distribution cell 4 . 3 . 1 . 1 .n a l y s p r i n c i p l e A is 4.3.1 Diluent lysing .2. action and 4 . 3 . 1 . 3 .o l u m e t r itc d y . . . . . . . . V s u . 4 . 3 . . 4 .R e s u l t s 1 .......... 4.3.2. Distribution Cells of RedBlood 4 . 3 . 3P l a t e l e t d i s t r i b u.t.i.o.n . . . . . . . . . . . . . 4.4. FLAGS STUDY GENERAL OF 4 . 4 . 1P l a t e l e t f l a g s . 4.4.2.HGBflag............ 4.4.3. Eosinophilflag 4.4.4. capabilities Comments theflagging on
. . . . . .2 . ................ 3 .......... 4 . . . . . . . . . . .4 . . . . ......................... 5 .......... 6 ....... I . . . . . . . . . . . .9 . . . . ............... 9 11 ................. 11 .................. 11 ..,............ 11 ......... . . . . . . . . . . . .1.1 . . . . .....12 1 . . . . . . . .2 . . . . . . . . .1.2 . . 13 ............. . . . . .1 3 14 .......,... . . . . . . . . . 1.5 . . . . . . .1 5 . 15 .......... 15 ...........
qq
4. TECHNOLOGY
HYDRAULIC 4.1. through a commanded the maincomputer by is system completely TheABC Vet hydraulic printed circuitboardinterface. A blockQ) cycle. dilution usedfor the analysis system 1 the Thediagram shows hydraulic Thisblockincludes sampling a tubes. the through hydraulic reagents aspirates delivers and syringeA,adiluentdispenserBandalysesyringeC.Bloodsamplesareaspiratedthrougha /:\ ($ whichis moving chamber andthe RBCchamber overthe mixing needte sampling Q,} Q is to sample transfered theWBCchamber of . fn" first dilution theblood @ of Ot means
generated the wastechamber in vacuum Q places.
. liquiOvalves route fluids the required the to
Diag.4-01
4.1.1.Sample drawinq
12pl blood needle and down thesampling Thedilution block @ aspirates ofwhole Q moves from sample tube. the
4. TECHNOLOGY
Diag.4-02
4.1.2. Samplingneedlerinsing Theneedle Q while dilution rises it is rinsed the Otock and Q moves (the needle up sampling
The for is in the sample). diluent used therinsing aspirated the doesnotmove itcontains blood as wastecnamOerQ.
Diag.4-03
4. TECHNOLOGY
4.1.3. Ghamber and needlerinsinq TheWBCchamber @ chamber Q needle while sampling the 'r drained @ tou", downinto mixing the
blockQ . fn. dilution
side the delivers diluent rinse external of the to again it drained theWBCchamber into . fn" WBCchambe, Q
Themixing chamber time. needle thesecond for sampling S Q
generated thewaste into chamber Ot mean thevacuum of Q
into cnamOer is drained thewaste @
Diag.4-04
Firstdilution 4.1.4. When dilution block the Q part chamber is the moves thefirst ofthediluent sentinto mixing up,
part needle block the Q thenpushes @ . fn" dilution @ tnrougn external ofthesampling part is sample pushed as syringe thesecond of thediluent wellas theblood and thesampling of of chamber . n totalamount 2.20m1 needle through sampting the @ @ intothe mixing ratio The is diluent delivered. dilution is 1/183. of introduced thebottom at of by withthediluent means airbubbles is mixed Theblood sample themixing chamber @ WBCchambe, Q generated thewaste chamber by , tror thepressure Q ,t drained thewaste. to the through
chamber . Thewaste Q
4. TECHNOLOGY
Diag.4-05
4.1.5. Second aspiration RBCchambe, @ The block isdrained. dilution Q moves down thebottom aspirates to and
needle moves so up The block 27,Sytlof firstdilution. sampling the @ tou". up.Thedilution its of to needle clean external to delivers 0,84m1 diluent thesampling dispenser thatthediluent from moves the RBCchambe, . Liquids aspirated the into needle are Thesampling side. @ of by RBC chamber means vacuum. chamberto waste the
Diag.4-06
q9
4. TECHNOLOGY
dilutionand transfer 4.1.6. Second chamber in part The remaining of the firstdilution the mixing Q
/':\ chamberQ)
't transferred the WBC to
in usingthe vacuumgenerated the wastechamber(S .nt the sametime,the
dilution block Q
into lyse O.56mlof solution theWBCchambe, moves anddelivers up Q
1/300. Thedilution becomes ratio usedforthe (n) detivers firstpartof thediluent the block Thediluent of dispenser thedilution needle side dilution theexternal ofthesampling on second @ ($ into RBCchamber the anO and
blockQ syringe the dilution of partof the diluent the through sampling the second
Atotal dilution. amount of sample thefirst out needle through sampling the Q rinsing theblood ratio The is of 3mlof diluent delivered. dilution is 1/20000' chamber into Pressure generated thewaste is Q needle Thesampling Q to ,o drain liquids thewaste. the .
cnamOer tou", overthemixing @
Diag.4-07
4. TECHNOLOGY
4.1.7. Counts withdiluent whichis aspirated using vacuum the heads rinsed are RBCandWBCcounting generated thewastecnamOer . into Q into Vacuum again is by of applied the is to Waste chamber drained thewaste means pressure. heads the WBC of of through counting the waste to chamber startthe aspiration the sample ($ chamber Q) andthe RBCchamber . is during firstcount the through WBCcnamOer . the Hemoglobin measurementtaken Q (duration seconds), halfsecond is on 8 a backflush applied When firstcountis completed the A chamber again. second countis carried heads thewaste and bothcounting @ ,. drained withdetergent whenthe second countis headsare rinsed and out for 8 seconds counting completed.
Diag.4-08
4. TECHNOLOGY
Rinsinq 4.1.8. anddrain TheWBCchamber O Thedilution block Q generated thewaste into chamUer the using vacuum ', drained Q .
part needle the moves andrinses external of thesampling up @ , tn" to 'r transfered theWBCchamber Q . fn" WBCchamOer Q the using vacuum
in chamber diluent themixing Q
generated the wastechamber into Q needle chamber . fn" sampling @ @ fromthedilution block Q
is drained the waste into
is rou". intothe RBCchambet @ . Diluent sent
part into to theexternal of theneedle the RBCchamber.
Diag.4-09
4. TECHNOLOGY
4.2.MEASUREMENT PRINCIPLES principles 4.2.1. RBC/ WBC/ Plt detection generated the passage cells principle based an impedance variation is of Thecounting on by micro-aperture. through calibrated the (current in diluent conductor). conductivity the The of 1 - Thesample diluted an electrolytic is from of cells. diluent differs considerably thenonconductivitytheblood micro-aperture. electrodes placed Two on 2 -The dilution pulled is the are through calibrated passes Electric current through electrodes the continuously. eachsideof theaperture. (or resistance impedance) the the electric between 3 - Whenthecellpasses through aperture, proportionately thecellvolume (Diag.a-10). with increases twoelectrodes
SOLUTIOH AHALYSER A SOLUTIOH EE AHALYSED TO

VACUUM= COI'ISTAHT
W
AHALYSIHG lulODUl--E Diag.4-10 lTlrulErfEltFsl
Wecanderive fromOhm's : law U = Voltage
U = Rl l= Current R = Resistance
(through aperture), U increases with the I R thus Since is constant, increases eachcellpassage proportionately cellvolume. tothe
4. TECHNOLOGY
so which amplification increases, the circuit have impulses a verylowvoltage, 4 - Thegenerated (Diag 11). noise the themandeliminate background can system analyze thattheelectronic
TIME
Diag.4-11
of circuits separately carryoutthe analysis white and chambers detection 5 - Twomeasuring cells. and blood cells, thatof platelets redblood and measurements thefollowing: are for different used these 6 - Thedilutions . WBC= 12plof whole is 83, the dilution at 1/1 then0.84 with mixed 2.20mlof diluent, final blood resulting a finaldilution 1/300. in of before count, the mlof diluent 0.56m1 lyseareadded of and in resulting a dilution with are at " RBC Plt=27,5plofthedilution 1/'183 mixed 3mlofthediluent, I of 1/20,000. which by RBC,Plt)is analyzed the micro-processor alsohandles 7 - Eachtypeof cell(WBC, (ABCVef16phistograms). thecelldistribution performing which the electronics, avoids useof the 8 - Tocount platelets, ABC yetuseshigh the generated therear impulses at sideofthe of for hydraulic systems theeliminationfaulty complex aperture. impulses a height with comparable zone, creates this the Whenredblood cellsre-enter analysis impulse The uses shape. instrument a verysophisticated impulses, witha different but to platelet platelet shape. which doesnothavethetypical rejects impulse any Thissystem sorting system. hydraulic system. and aperture a traditional maintainsveryreliable a Thissorting system g - ABC Vet 16pprovides for channels the by on curves, analysis 256 counting distribution for WBC,RBCand128channels Platelets.
4. TECHNOLOGY
principle 4.2.2. Hemoqlobin measurement is including blank 2 measures blank sequence cycle HGBReference an 1 - During STARTUP the is is twomeasures tooimportant thirdmeasure performed. a these run.lf thedifference between HGBblank out and to 2 - Every cyclean Hgbblankis carried on diluent compared theprevious analysis. This lyseagentis added the3,04mlof1/300 to dilution. reagent 3a (ABC VetSP) 0.56mlof potassium andpotassium ferricyanide cyanide contains ]K IKCN]. [Fe(Cn) is This lyseagent added the3,04mlof 1/300 to dilution. reagent 3b (ABC Vet16P) 0.56mlof no contains cyanide. with freedby the lyseof the redblood cellscombines the 4a (ABC VetBP) The hemoglobin potassium cyanmethemoglobin compound. to cyanide formthechromogenous freedbythelyseof the redblood to cellsis stabilized forma 4b (ABCyef 16P)- Thehemoglobin compound. cyanide-free chromogenous part by through optical of theWBC the is 5 - Thecompound thenmeasured spectrophotometry, of with chamber, a wavelength 550nm. withtheunits up inthesection set 8.1.3"lnstrument configuration". is 6 - Theresult given will to sequences becarried previously theanalysis come, out to blank NOTE I HGBreference : if the operator - hasrequired CALIBRATION operation. a - hasleftthe instrument morethan 10 minutes afterthe STARTUP. - hasleftthe instrument morethan60 minutes afterANALYSIS. - hasnotcarried the STARTUP cycleafterswitching the instrument. on out principle 4.2.3.Hematocrit measurement is generated the passage a cellthrough micro-aperture by of the 1 - Theheight the impulse of proportionalthevolume theanalyzed of cell. to directly of integration theMCV. is as of 2 - Thehematocrit measured a function thenumeric set 8.1 configuration". is withtheunits up inthesection .3 "lnstrument 3 - Theresult given 4.3.CELLDISTRIBUTION STUDY (histograms)forWBC RBC 256analysis on and outvolumetricdistributions ABQVetl6pcanies range forPLT withthefollowing measuring : and channels, 128channels . WBC= approximately to 460fl. 30 . RBC= approximately to 300fl. 25 * Plt= approximatelyto 33 fl. 2 4.3.1. Whitebloodcell distribution : the threeleukocyte sub-populations studyreveals following TheWBCvolumetric distribution Monocytes, Granulocytes. Lymphocytes, principle 4.3.1.1. Analysis and diluent studyof leukocytes useof a (patented) after is on Theprinciple based thevolumetric reagent. a lysing
4. TECHNOLOGY
4.3.1.2. Diluent and lysingaction The reaction. lysehasa for the preserves prepares cellmembrane thedifferential and Thediluent membranes The cytoplasmic membranes. lymphocyte on mode action cytoplasmic of differential nucleous, the around the the and cytoplasm shrink membrane soluble of allow release water the granulocytes a limited reaction due have while reaction, the an monocytes undergo intermediate of action whichprotects themfromtheshrinking structure and to a molecule theircytoplasmic the lyse. 4.3.1 Volumetric .3. study coming of analyzes heights eachimpulse, the ABC vet16p lysing action, Afterthedifferential with of is A channels. curve obtained number in fromtheaperture ranked the256counting and on and cells they-axis cellvolume thex-axis. on ( Diag. 4-12.) : downaccordingly Thecells be broken will . the lymphocytes 30 between - 100fl . * themonocytes 100 between - 150fl . * thegranulocytes (unlimited volumetrically). 150 between fl anda maximum
(um3)
Diag.4-12
curve. in zonesin the distribution placethemselves different Pathologic will,of course, cells elements. of of flagswillalertthe laboperator the presence suchpathologic Mobile fixed and (see is when option validated ticket this out is (seesection 4.4.). Thiscurve printed ontheresult section 8.1.2). lower than having DILflagor a WBCresult a out will NOTE I Thiscurve notbe printed for results 1000. 4.3.1.4. Results (%) in are and monocytes, granulocytes expressed percents and absolute The lymphocytes, GRA%, GRA#. MON#, MON%, (#): LYM#, numbers LYM%,
4. TECHNOLOGY
4.3.2. Distribution RedBloodCells of linked anisocytosis. erythrocyte anomalies to of Thestudy thedistribution RBCdetects of you (RDW)will enable to follow evolution thewidthof the the of Width A RedCellDistribution volume. and in relation thecellnumber average to curve KSD RDW= MCV With K=systemconstant according statistical to deviation studies celldistribution. on standard SD= Determined = Mean Volume erythrocytes of MCV Corpuscular gi.2). (see when option validated section is ticket this Thiscurve printed ontheresult is out 4.3.3. Platelet distribution platelets, plalelet detects anomalies alerts lab and the The platelet studycounts distribution (schizocytes, cellpopulation microcytes, seesecetc..., in of operator theevent a non-platelet (see whenthisoption validated section ticket is is out Thiscurve printed ontheresult tion4.4.). 8.1.2). placed 2 fl anda variable threshold. The at high a 1 - Platelets counted, between lowthreshold are population, in whichis present the to the microcyte varies according variable highthreshold platelet (seeDiag. 4-13). zone analysis
Diag.4-13
Platelet Volume) directly is fromtheanalysis derived 2 - Measuring MPV:TheMPV(Mean the platelet MPVis expressed pm3 fl. in or curve.The distribution of the PCT: 3 - Calculating PCT"/"=
PLT(103 x MPV (fl ) /pl)
10000 Width) Thiscountis derived Distribution : fromthe platelet 4 - Measuring PDW(Platelet the curve.
4. TECHNOLOGY
Diag.4-14
from2 fl (S1)and of starting 15h ot the number platelets PDW= Width thecurvebetween of (S2). top platelets withthevariable threshold beginning of 15/"ofthenumber
FLAGS 4.4,STUDY GENERAL OF generalflags occur: may the Withtheprint-out results following of - * following three the analyzed sample that or HCT PLTindicates thesystem WBC,RBC, either precision The limits. result the andwereoutside systems differed counts times thatallthree but the by should verified repeating sample' be - $ , placed indicates two of the three that the and preceding parameter afterthe test number limits: werein following counts - 7"/"tor WBC - 5%for RBC - 15%for PLT are lf oneof thetwofirstcounts under: - 3000for WBC:The limits 97" becomes - 16000 RBC: Thelimits 87o becomes for - 400for PLT:The limits becomes20h parameter be reported. can The result the concerned for
4. TECHNOLOGY
- -- D printedbehinda resultindicates the linearity has that rangefor that parameter been exceeded. and each timetheflagreappears. Repeat sample using 1i:2dilution repeat a the shows thevalueis above upperlimitsetupby that the H : located to a result a parameter next of (see 8.2.2). theoperator section shows thevalueis below lowerlimitsetup by that the L : located nextto a result a parameter of (see 8.2.1). theoperator section
IMPORTANT
in they are initialized Linearitylimitsare ditferentfrom a specieto another, the the instrumentwhen downloading smart card.
4.4.1. Platelet flaqs (Displayed printed : seesection 6) out and - MICfollowing Platelet presence microcytes the Platelet in result indicates excessive the of the (PRP) a manual This RichPlasma using Platelet a or count. Verify result the measurement zone. 8.2.4). flagcanbe adjusted theuser(seesection by - SCHfollowing Platelet result indicates presence schizocytes Platelet the or aggregates of the result. Thisflagcan be measurement zone.Review/scan beforereporting slide in the Platelet 8.2.4). by adjusted theuser(seesection - SCLfollowing Platelet A indicates presence small result the cellsinthe2 and3 fl zone. of the persist, lf out verified. thisflagshould should carried andthe results be second sample cycle perform automatic lf verifyusinga Platelet cycleand resample. the flag persists cleaning an (PRP) thesample make manual for This and a slide count thePlatelets. flagcan Rich Plasma of 8.2.4). be adjusted theuser(seesection by 4.4.2. HGBflaq (Displayed printed : seesection 6) out and - ! : located to theHGBresult and shows theHGBblank that carried during analysis out the next precision limits. differed wereoutside system's and the blank theprevious analysis provides result Thisresult a HGBblank. according theprevious to Nevertheless instrument the can be reported. 9.2.3.3. run to more thanthree consecutive timesrefefto section lf thissuspicious occurs flag procedure. thecheckup 4.4.3. Eosinophilflaq - EOS( ) : located percentage high.Limitis indicates Eosinophil an nextto WBCalarms to by to adjusted al2/", thisflagcanbe adjusted theuserfrom0.01% 25.5h. 4.4.4. Comments the flaqgingcapabilities on manually signaled theABC Vefshould verified by be Allanomalies abnormaldistributions and/or As resistance cytoplasmic of of elements. a result thedifferential forthepresence pathological of elements can be foundin a numberof in cell membranes the different types,pathological to of or cells Thisalsoapplies thepresence normal non-pathological thathave zones. different in Thiswillresult formof treatment alarm in zones. or beensubject chemotherapy someother to a false alarm.
q9
5. DESCRIPTION
5. DESCRIPTION
5.1.INSTRUMENT
Diag.5-01
1 2
3 Cover part 4 Doorto pneumatic
door carriage SamPling panel Front
1 - ABC VetCover (seescrew section locations Diag.2.4 on of Theinstrument coverisfixedbythemeans 5 screws dooron the access openthe pneumatic the to 2.7).Betore attempt remove cover, any . frontofthesystem parts. alsoallows the lt to Thisdoorgivesan access the pneumatic door. access 2 - Pneumatic to lt is recommended keepthe doorlocked cycleoperation. to operator checkthe hydraulic interference shield. with as cycles it is equipped an electrical during measuring the and lts blood sampling. internal forthel2plwhole is Thisneedle used needle. 3 - Sampling it has instrument to be moved, is recommended Whenthe is rinsing fullyautomatic. external to position door the through pneumatic in order avoid in lift to manually upthe needle itsupper anydamage. : with to panel. the allows operator communicate the instrument Thispanel 4 - Control - To access different cYcles the - To identify Patients the - To setup instrument, etc... the
5. DESCRIPTION
Frontpaneland commandkevs 5.1.1. A - Usingthe keyboard havebeendispatched "menus". in Eachmenuhasa cerAll functionalitiesthe instrument of (see OVERVIEW These menus allaccessible section 9.5). tainnumber functions MENU of are Navigation themenus done is at startup. in through MAINMENU the displayed theinstrument
k"yt command ffi using ">"cursor the
and m
. Entry a function done in is by

key.
in and tne moving cursor frontof thefunction pressing @ the
NOTE
it are to Whenmenus and functions known, is possible enterdirectly menuor the it ENTER whena function number access (andpress is function to
Diag.5-02
1 2 3 4 5
key Startup Standby key key ldentification cyclekey Startanalysis Numerical keyboard
6 7 8 9 10
Delete key Enterkey Escape key Display scrollkeys Cyclelightindicators
qq
5. DESCRIPTION
B - Commandkeys a key 1 - Startup : Whenthiskeyis pressed, startup procedure is a and cycleincluding cleaning rinsing is rinsed left out. carried Detergent in thechambers is for and withdiluent thesystem ready the analysis of Thiscyclehasa duration approximately cycles. (thiscyclecan be run 2 or 3 timesif 130seconds limits). the are blankvalues notwithin acceptable
at 2 - Standby key : this key is usedfor shutdown this day. theendoftheworking When keyis pressed, to it is mandatory carryout a startupcycle before of cycle.This cycle has a duration any analysis 65 approximately seconds.
identification the 3 - Thiskeyis usedto enter patient and (13 characters maximum, letters numbers), or the runnumber.
key cyclecommand : Thiskeyactivates 4 - Analysis whole blood the cycle andaspirates 12pl thesample needle in is When sampling the for sample analysis. on the its upperposition, firstpressure the keywill pressure start will lower needle thesecond the and the analysis.
m m ffi m m ffi ffi ffi ffi m G m

ffi+#
: keyboard Keysfrorn0 to 9 allowthe 5 - Numerical figures : to operator enterthefollowing - Date - Calibration values - Laboratory limits - Patient number analysis for - Leukocytes flag differential values.
will simultaneously allowan These2 keyspressed of adjustment the LCD. automatic
5. DESCRIPTION
6 - Whenit is pressed, keydeletes informathis the tionentered the LCDdisplay. on
7 - Thiskey is pressed validate informations to the entered the LCDdiplay. on
I - Whenit is pressed, key allows operator this the to exita function to comebackto the previous and menu.This key can be usedto stopan hydraulic cyle(seeNOTE below).
ffi ffi
keysallow userto scroll or downin 9 - These the up t t h e i n s t r u m e nm e n u st o a c c e s st h e d i f f e r e n t functions to choose letters thepain and alphabetic tientidentification.
@
NOTE Whenthe STARTUP
10- Whenthe<start> is activated, indicator key the lightflashes during sampling the time.Whenthe indicator is to stopsflashing, operator allowed the position. remove tubefromthe sampling This the cyclehasa duration 100seconds. of
is pressed duringan hydraulic cycle,it is necessary run a to to rinse instrument the before analysis. any
C - Screendetails
t ,
t! Jr
t;. t:
if,:::::: :::t:i:::t: l
is The Theposition thecursor givenby the">"as shown above. 1 andy on the rightside of indicate moremenus available anddown. are up of thescreen that
5. DESCRIPTION
Rearpanell Mainfuses 5.1.2.
or
I I I I I
: O
I I
I . I
I a
Diag.5-03 1 2 3 4 panel Rear connection 5 RS232computer 6 Printer output ON/OFF switch F1,F2 Main fuses Mainpowersocket
-@: ur - asov
lDlar lntd.ioa Fot*rr3
*TfiT',ffi'@^@
lmide fusos
Diag.5-04
5. DESCRIPTION
5.1.3.Left side internalview
Diag.5-05
1 2
syringe Vacuum/pressure block1 Liquidelectrovalve
3 4
Dilution block Liquidelectrovalve block2
5. DESCRIPTION
5.1.4.Frontinternalview
Diag.5-06
1 2 3 4 5
LCDdisplay Keyboard Cyclelightindicators needle carriage Sampling Startcycle key
6 7 8 9
Mixing chamber RBCchamber Spectrophotomer WBCchamber
AND SAMPLERUN 6. STARTUP
6 . 1 S T A R T U PH E C K S . C set Bottles containers 6.1.1. and pack Reagent 6.1.2. R 6 . 2 P R I N T ES T A R T U P . 6 . 3| N S T R U M E N T S T A R T U P . . . . . . . . . . . . ANTMAL BLOOD TYPE SELECTION 6.4. MIXING 6.5. SAMPLE COLLECTIONAND 6.5.1. Sample collection VERIFICATION ............. DATLYQUALTTYCONTROL/CALIBRATION 6.6. identification 6.6.1. mode US mode identification 6.6.2. Standard R G 6 . 7 . U N N I NS A M P L E S d o. 6 . 7 . 1S a m p lie e n t i f i c a t.i . . n. . . . . . . . . . Automatic cleaning 6.7.2. 6 . 7 . 3E n d ft h ed a yr i n s i n g . o 6 . 8 . 1A B CV e t8 p . A 6 . 8 . 2 . B CV e t1 6 p
. . . . . . . . 2. . . 2 .".'..'.-" ."'.'."-."2 ...........2 ............2 3 ........................ 4 ....................... 4 ....'.. ............. 5 5 ...............". .'.'...... 6 .........7 ...."..7 7 ...............'........ ...""...'--."...7 . . . ' . ' . . . . . .I' . " . . . . . ' . ' . '1.1 . .
qq

6.1.STARTUP CHECKS Bottlesand containers set 6.1.1. reagent levelis low,replace and primesystem lf Checkthe levelof eachreagent. reagent 2.13. in described section according the procedure to
NOTE
present the bottom Particles in of pourreagent to fromonecontainer another. Never newreagent, causing unacceptable background the can theoldcontainer contaminate for counts, especially PLI-s.
paper. more printer paper needed, is replace lf the Empty waste and the container check printer paper. theprinter pack Reagent 6.1.2. of is by The lowlevel the reagents indicated the number CBCsleft(from5 to 0) whenthe of cycle: operator to runan analysis tries
the section 6.6)pressing either runthe cycle(as described to The operator the choice has packby a newone(seesection 2.13). keyor to replace Reagent the m 6.2.PRINTERSTARTUP (seesection 2.12.). LEDare Check thecontrol that of Press theON/OFF switch theprinter on
oN.
6.3.INSTRUMENT STARTUP The located therearpanel. display shows switch on the TurnONinstrument pressing ON/OFF by : thefollowing
phase panel that fromredtogreen indicate theinitialization iscompleted. to LED Then front turns the

(seesection for of 8.3.3 thesetup theautomatic cycle startup Waitfortheendof theautomatic andperform will a cycle key, instrument perform startup the or the startup cycle) press @ without blood any sample). on cycle reagent count(ananalysis for a blank cycle a background : the doesnotexceed following count Check thebackground that - WBC: - RBC: - PLT: (0.2 0.2 t o3/mm3 on thedisplay) (0.01 thedisplay) on 0.01t o6/mm3 (5 5 103/mm3 on thedisplay)
perform will another STARTUP values, instrument automatically the lf theresults above these are is "Startup failed,checkreagents" persists after3 cycles,a message cycle.lf the problem procedure. to 9.2.2.2 runthecheckup refer displayed, to section out. lf this one is not within blankis carried During STARTUP cyclean HGB reference the failed, and checkreagents" "HGBReference a valuesafter3 cycles, flag "Startup acceptable procedure. to 9.2.3.3. runthecheckup to resultsrefer section failed" printed withSTARTUP is out ; until FAILED" printed withallthe results a is "STARTUP out lf theSTARTUP themessage fails newSTARTUP carried is out. it to 4 has When instrument notbeenusedforat least houres, is possible setupan automatic the (auto. is When option activated mode). this (seesection 8.3.4Inactive runof a STARTUP cycle whichever This occurs of every mode) STARTUP isrunautomatically 4 hours inactivity. cycle cycle a (except SERVICE menu). the menu displayed theinstrument is on the value ifthe the NOTE I During STARTUP, HGBREFERENCE is unacceptable, background run is out countis notcarried anda newattempt directly (3 maximum)'
BLOOD TYPE SELECTION 6.4.ANIMAL with smart card. typerecorded a veterinary in the Theinstrument keeps memory lastanimal informations : the Each veterinary cardcontains following smart - Typeof animal - Highandlowlimits each parameter for - PLTandEOS(ABCVet16p) thresholds flag - Sample lyse volumes and - WBC,RBC,PLTcoincidence values coefficient - Calibration HGB,HCT, PLT forWBC,RBC, coefficients adjustment - DlLflaglimits WBC,RBC, PLT HGB,HCT, for the into if is 1 - Whenthe initializationcompleted, no smartcardis present the cardreader, : is following menu displayed
<2> next is Theanimal typerecorded displayed to thefunction VETERINARY.
lJonaAlQ
qq

Cycle or in cardreaderwhenyoupushStart 2 - lf aveterinary smartcardis present thesmart load key,thiscardis automatically in the instrument. Start
Diag.6-01
NOTE
At anytime,beforeyou run a sample,you can inserta smartcard to changespecie.
NOTE
this the lf you manipulate cardduringthe sampling will interrupt to and initializeparameters default(DOG). the numeration
AND COLLECTION MIXING 6.5.SAMPLE 6.5.1. Sample collection by or sample blood themeans vacuum atmospheric of has Sample collection to bedoneonvenous with volume 100;.tl of bloodintoa microtainer a minimum lt to collection tubes. is possible collect K3. the used this andanalyze on theABC Vet.lnallcases, anticoagulant hasto be EDTA
IMPORTANT
The sample collection tube has to be filled with the exact quantity of bloodsample blood indicatedon thetube itself.Any incorrectlymeasured collectionwill show a possiblevariationin the results.
for whicharehighly recommended the tubes collection manufactures sample ABX Diagnosfi'cs has a listof recommended sample in use of ABCVet(notavailable US).ABX Diagnostics at collection tubes yourdisposal. Mixinq 6.5.2. (witha gentle anddownand rolling mixed up and Blood mustbe thoroughly gently samples motion), before measurements. any

VERIFICATION / CONTROLCALIBRATION 6.6.DAILY QUALITY levels control of that analyzes three it samples is recommended theoperator Before analyzing is limits. that acceptable (low, to normal high) verify thesystem within and material : modes available are Twoldentification identification eachanalysis. on the 1 - USmode: thatrequires patient a on mode: incrementsrunnumber eachanalysis. 2 - Standard mode) (seesection the to 8.3.7. select identification 6.6.1. modeidentification US
^ Pressthe CJ3*) menu is key of the front panel to enterthe controllD. The identification
displayed:
letters numbers. Letters be entered or can using13characters, Theidentification beentered can Press ENTER eachletter stepto for to keysof thefrontpanel. the using m O "no (orESCAPE to save key is entered key the thenextone.Press ENTER whentheidentification BAR THE one) thedisplayed : a message'PRESS SAMPLING " is displayed.
Diag.6-02 the behind bar on Present sample shown Diag.6-02and pressthe sampling located as the rey. tne needle press @ or sampling
qcl

whenit is notusedduring firsthour the risesup after10 minutes needle NOTE I Thesampling key)will lower the on bar The of functioning. firstpressure the sampling (or @ pressure starttheanalysis will needle, second the
ldentification in memory stays cyclehasnot beenrun,the current the NOTE I Untit analysis whenpressing @ r."v. the on andcan be recovered the screen to the flashing, LEDturns red,remove tube. the indicator Whenthelight stops printout, LEDturns green theinstrument ready thenext is for to and the At theendoftheresult analysis. perform following ranges : the the results outside acceptable are lf anyof thecontrol a - Re-run control the (seesection 9.1) the and the b - Clean system re-run control c - Opena newvial (seesection 7) the d - Recalibrate system mode identif ication 6.6.2. Standard Press @ the Run#. Thefollowing menuis displayed : keyto enter control the
keysof the frontpanel. Press the (from'1to 9999)using numeric the Enterthe run number (ortheESCAPE to savethedisplayed :A message key one) ENTER to record number key the is "PRESS BAR" displayed. THESAMPLING 1 the bar behind sampling the on Present sample shown Diag. andpress sampling located as the
needle press ffi or tne
key.
is for printout, LEDturns green theinstrument ready thenext and to the At theendof theresult analysis. the to flashing, LEDturns red,remove tube. the When light indicator stops the perform following ranges : the the lf anyof thecontrol results outside acceptable are the a - Re-run control (seesection 9.1) the and b - Clean system re-run control the c - Open newvial a the d - Recalibrate system

6.7.RUNNING SAMPLES
6.7.1. Sampleidentification next check theanimal displayed tothefunction that type up After the completing start procedure, Run to to 2 VETERINARY corresponds the bloodsamples be analyzed. a non pathological anaby control analyses thenthesample and day, fromtheprevious followed thequality blood lyses. (US or to either sampleidentification mode) the section 6.6.2. enter 1 - Proceed described as mode). thesamplerun # (Standard the behind the bar on as 2 - Present sample shown Diag.1 and press sampling located the t<ey. needle press @ or ttre sampling Automatic cleaninq 6.7.2. procedure from cleaning an has When instrument run50samples thedatechanging, automatic the while menu approximately theautocleaning isdisplayed lasts minutes cycle 3 iscarried This out.
*yJ.o-.qs*l1'$$..-i.
NOTE
PLEASE 'MNS wArr
frequency beadjusted theuserfrom1 to 50as described can by cleaning automatic 8.3.4 setup(section in the instrument
Endof the day rinsinq 6.7.3. key, the the cycle It is necessary runa standby/shutdown at theendof theday.Press @ to by into performs complete withdetergent, putsthesystem thestand and cleaning instrument a iftheworking is completed leftinthisstandby or off day The can mode. instrument beswitched next analysis. mode or overnight untilthe is Whenthe instrument before
S
NOTE
mode,it is necessary carryout a startup to
6.8.RESULTS to results displayed printed according the and out are Whenthe analysis cycleis completed, : of setuo theinstrument - Result (seesection 8.1) setup - Date (seesection 8.4.2) format - Sample (see 6.5.2) identificationrunnumber section or - Unitsetup (seesection 8.1.3) - Possible 8.2) see flags(lablimits section

6.8.1.ABC VetSp
A - Displayed result : results displayed Theparameter are
* Identification 1. US mode : whenmoving cursor the bottom the to Thesample identification be reviewed can
'ffiffi
uri,':.*rr, ,l& ,.ffi;:i %',o', ,:i$.,
2. Standard mode whenmoving cursor the bottom the to run Thesample number be reviewed can
* Flags : to moving cursor the bottom the The PLTflagscan be reviewed
from again anytimewhenmoving cursor at the run NOTE I Thelastsample canbe displayed neSUf-fSandpressing ENTER. the mainmenu thefunction to Q
in 4.3. with described section easily theflags Abnormal values be identified can

B - Resultprintout 1 .U Sm o d e
RESULTS
(4 HDOG
v . W g t
lwnr: WBC RBC HGB HCT
_ \:-/
@ \Y-l
10.0
10
MCV MCH MCHC PLT
30.6 34.1 287
Pg gldl 10t/mm'
Diag.6-03 : printout (ABGVet8p)canbefound(seeDiag.6-03) Onthe result was run. 1 - Thedatesample wasrun. 2 - Thetimesample by that identification wasentered the operator. 3 - The sample 4 - The selected specie. 5 - The PLTflagsif any. flags' with 6 - TheCBCresults theircorresponding number. 7 - Thesequence status. 8 - TheSTARTUP Thisonecan by and to is number updated 0 everyday increases 1 on eachcycle. Thesequence notbe modified the operator. by
qq

2. Standard mode
RESULTS
DATE: 11/09/98
WBC RBC HGB

|-|L, I
1O.O 4.90 15.0 43.9
10'/mm' 10u/mm' g/dl YO
MCV McH MCHC PLT
30.6 34.1 287
Pg g/dl 10'/mm'
Diag.6-04 printout (ABC Vet8p)canbe found(seeDiag.6-04): On the result 1 - Thedatesample wasrun. was 2 - Thetimesample run. by run that 3 - Thesample number wasentered theoperator. 4 - Theselected specie. 5 - The PLTflagsif any. flags. with 6 - TheCBCresults theircorresponding 7 - Thesequence number. Thisonecan and by Thesequence is to number updated 0 everyday increases 1 on eachcycle. : notbe modified the operator. by

ABC Vet16p 6.8.2. result A - Displayed : is of Thefirstgroup parameters displayed
to whenmoving cursor thetop : the group parameters beaccessed can of Thesecond
* ldentification 1 .U Sm o d e to whenmoving cursor the bottom the can Thepatient identification be reviewed
mode 2. Standard : to the whenmoving cursor thebottom run can Thepatient number be reviewed
j H#iffi*, T-',yF
* Flags:
lt::i::;
to the moving cursor thebottom can ThePLT andLMGflags be reviewed
rs
fromthe the at again anytimewhenmoving cursor run sample canbedisplayed NOTE I tne tast ENTER. and main menu thefunction RESULTS pressing to O
4.3. in with described section easily theflags values be identified can Abnormal

B - Resultprintout 1 .U Sm o d e
RESULT'S
WBC RBC HEE HCT PLT
l : : ; :
tl .3 5.97 13.f) 3S.? 3bb
ltJE/rnm3 lDA/mms g/dL 7. l0F/mms
MCV MCH MCM RDW
pms : 65 : 21.BFpe H! 33.48q/dl I 13.I81/
WBC FLAGS: DIFF I TLYPf: LA ZI'lONr 5.5 %ERAr A6.7
B'I B ?/. H 7.
1.1 A.3 ?.9
B l$slmmF lOs/nrns H l0E/mms
Diag.6-05
: printout befound(seeDiag.6-05) can Onthe result 1 - Thedatesample run. was by identification wasentered theoperator. that 2 - Thesample number. 3 - Thesequence 4 - Theselected specie status. 5 - Thestartup 6 - TheCBCresults. 7 - The Diffresults. representations. 8 - Thehistogram was 9 - Thetimesample run. by and to number updated 0 everyday increases 1 on eachcycle.Thisone is The sequence by can notbe modified the operator.
RUN AND SAMPLE 6. STARTUP
2. Standard mode
REgULT'S
WBC : RBC : HEB : HCT c FLT !
11.5 5.97 13.O 58.9 26b
108/mms lQ-lmms q/dL 7. l0s/mmG
65 MCV I um= 21.BFpe MCH : MCMH: 33.48q/dl RDW: l3.Ln7.
WBC FLAGS: DIFF: TLYPI: 9. E 8 7 , Zl"lONr 3.3 8 7 . ZGiRAT A6.7 H Z
1.1 B lt)t/mrns 0. 3 lOt/rnrnt 9.9 H l0tlrnma
Diag.6-06 printout be found(seeDiag.6-06) : can Ontheresult was 1 - Thedatesample run. by run 2 - Thesample # thatwas entered theoperator. number. 3 - Thesequence 4 - Theselected specie 5 - TheCBCresults. 6 - TheDiffresults. representations. 7 - Thehistogram was 8 - Thetimesamole run. and by is to number updated 0 everyday increases 1 on eachcycle.Thisone Thesequence by can notbe modified the operator.
7. CALIBRATION
qcl
7. CALIBRATION
7.1. INTRODUCTION ways: in TheABC Vetcalibralion be achieved twoditferent can lt TYPE samples. requires CALIBRATION the blood 1 - Calibrationcarried withcalibration is out package be usedto enter into can Thecalibration contained thecalibration card smartcard. informations. directly calibration the product. ABX Diagnostics calibration Callyour to usetheMfNOCAL directly. andcanbe entered 2 - Calibration are coefficients known
IMPORTANT ment,blankvaluesmust be in the acceptable limits (seesection6.3).
instruhas to be done alwayson a clean and reproducible Calibration
From mainmenu, move cursor to the the TYPEcardintothereader. Insert CALIBRATION the menuis displayed : the <2> and thefunction VETERINARY pressENTER. following
rYPEcALlBRArloN : "S,,Sir?*
in contained thesmart type PressENTER confirm CALIBRATION andthe informations the to MENU, move cursor thefunction the to From into cardarerecorded the instrument. the MAIN menu : The and |3-.]CnLtenATtON pressENTER. CALIBRATION is displayed CALTBRATION HH:MM 1 AUTOCALIBRATION 2 COEFFICIENTS
7.2.CALIBRATION CARDAVAILABLE 7-01. as on cardreader shown diagram Installthe card CALIBRATION inthesmart
Diag.7-01
7. CALIBRATION
ENTER. and Move cursor function AUTOCALIBRATIONpress to the Q card, values recorded thememory into are date the As thelotnumber, expiration andthetarget : remain only2 operations - Theoperator 7.3.1, section described selection - Theselection thenumber thecalibrator 7.4.1 . samples described section of of 7.4.2."Run step calibration". out carry thefollowing section done, Oncethese steps two has as NOTE I Thecardcanbe removed soonas the lot number beenvalidated. before starting calibration. be type NOTE I Thecalibration must validate 7.3.CALIBRATION CARDNOTAVAILABLE and to movethe cursor function nUTOCALIBRATION press menu, Fromthe calibration [l the menu step the selection, The ENTER. CALIBRATION willunfold bystepthrough operator before values thenumber samples beanalyzed and of to the lot,identification, target calibrator the starting calibration. 7.3.1. Selectoperator (see of identification section 8.3.1setup the operator Move cursor oneof the4 required the to , press next identification A ENTER. star(.) isdisplayed to thechosen identification) and operator identification. to andthemenu turns thecalibrator
7.3.2. Changelot number
has lf of blood to bechanged, is lot Thecurrent number displayed.thelotnumber thecalibration : pressENTER. following menuis displayed The
ffi#;r;
('10 directly using maximum). Numbers be entered can Enter newlot number characters the keys.Foreach ano(T) using G the letters be entered can keyboard, the numerical pressthe ENTER to validate and enterthe nextone,validate second timeto it a key letter, has to theexpiration lf thelotnumber notchanged, date. The turns record lotnumber. menu the to date. press ESCAPE the menu turnsdirectly the expiration key, the
qq
7. CALIBRATION
7.3.3. date Chanqe expiration in set 8.4.2. Thecurrent date expiration is displayed theformat up in section (DD:MMIYY) EXP DATE? (DD.MM.YY) EXPCHANGE ' CURRENT: NO: ESC
values. theexpiration lf press escape themenu turnsto thetarget date key, lf it is correct, the press menuis displayed : ENTER, following the needs be changed, to
requested the display on and pressENto Change expiration according theformat the date valuemodifications. TER.The menu turnsto the target 7.3.4.Changetarqet values valueis displayed : WBCtarget Thefollowing menuwiththe current
il
' vg;,*3$"*o ,l 3i"
pressESCAPE, menu value. the lf the turnsto RBCtarget lf theWBCtarget valueis correct, pressENTER, following menuis displayed : the valuehasto be changed,
.'i ;tfigEi,Y," ,,i'

ENTER press ESCdirectly. menu The or value required press if and Enter newWBCtarget the for When and PLT. Repeat sameprocedure RBC,HGB,HCT, value. the turnsto RBCtarget (or to the of samples be thislastvaluehasbeenmodified ESCpressed), number calibrator is analyzed displayed. 7.4.RUNCALIBRATION 7.4.1. numberof samples Chanqe
The autocalculation on The calibration the ABC Vetcan be performed 3 to 11 analyses. of performs results order obtain bestcalibration in to the coefficients. on module statistics these
IMPORTANT
to ln order to obtainthe best calibrationpossible,it is recommanded run blood a minimumof 5 times. the calibration
NOTE
in calculations usedfor the The first resultis not takeninto account the statistic is The calibration. firstsample usedas a
7. CALIBRATION
(or ESC). following number calibrator the of samples press the Press ENTER change required to : menuis displayed
if or samples pressENTER required pressESC.The and of Enter newnumber calibrator the menuis displayed. runcalibration 7.4.2.Run calibration (temperature, instructions mixing, etc...). to according the specific Prepare calibrator the
: menuis displayed key.Thestartcalibration Press ENTER the RT CALIBRATION : T EXITCAUBRATIGN the Openthevialandposition sampling the Instrument requesting firstrunof thecalibrator. is the or Press sampling located bar behind needle press the the inside bottle. needle the deeply the Whenthe cycleLEDstopsflashing, remove vial located the frontpanel. on cyclebutton menuis Whenthe analysis cycleends,the firstresult the and replace cap on the calibrator.
displayed:
wBc
;x.x
PRES$ HNIEA. ].. TOCONT}NUE
givenin the calibrator instructions pressENand the are Check thatthe results within limits : menuis displayed TER.Thefirstvalidation , vAl-lD.CALIBRATION
EsCTO DTSCARD " ,,

limits, is possible reject results to restart it and to the the lf the results notwithin acceptable are the thefirstrunusing ESCkey. rejected. flags($ , * or ! for HGB)are automatically having NOTE I Results run pressthe ENTER key.The menuof the second calibration is lf the results correct, are : displayed
sienr cAtiB*Au lrt $ru nnn-r1t{ *sc ro'ExrfCAI
i.'#iEffi-;;^;;-;;' ;
of the until and sample follow sameprocedure thenumber samples Runthesecond calibrator the calculates the Whenthe last resulthas beenvalidated, instrument set up is obtained. : for factors eachparameter statistical calibration percentage difference between targetvalueandthe the of Mean, coefficient variation, target, coefcalibration as of coefficient wellas a recall theprevious newcalibration mean(% CHG), printout. on appear the result status ficient a pass/fail and
qq
7. CALIBRATION
passed 7.4.3. Calibration limits : the lf thestatistic figures within acceptable are - Coefficient variation within limits given section and, in 3.1 is the of - Thepercentage value less is 20 and than between target themean the difference passed the results printed Thecalculated become the out. coefficients are and thecalibration is and newcalibration coefficients thecalibrationcompleted.
PARAMETERS
wBc
2.5
RBC
HGB
t"tcT
2.0
PLT
MPV
Tab.7-01
CV
2.0
3.0
NOTE
( in is calculations Thefirstrun of the calibrator nottakenintoaccount the statistic "P"is out nextto the runnumber). CALIBRATION DATE:04102194 J O P E R A T O:RM G N LOT r:6115N RUN 1 P 2 3 4 5
o
TIME09:21 :
WBC 6.3 6.3 6.4 6.5 6.6 6.5
RBC 5.30 5.35 5.40 5.3'1 5.28 5.30 5.32 5.34 0.89 0.37 0.90 0.88 OK
HGB 16.0 16.1 16.2 16.1 16.1 16.0 16.1 16.1 0.43 0 '1.'13 1. 1 3 OK
HCT 47.2 47.1 47 46.8 47.5 47.1 47.1 47 0.54 0.21 0.98 1.03 OK
PLT 230 224 230 240 228 235 231.4 221 2.69 4.70 0.91 0.95 OK
6.46 MEAN TARGET 6.5 1.76 cv 0.61 % cHG OLDCAL 0.98 CURRENT0.97
Diag.7-02
STATUS
OK
: is Thefollowing message displayed
Press keyto return the MAINMENU. to a 7.4.4.Callbrationfailed limits, following menuis displayed: the the lf the statistic figures notwithin acceptable are
- Coefficientvariation notwithin limits given thetable in 7-01 above or, is the of - Thepercentage 20 mean valueisgreaterthan and between target the the difference the out. coefficients rejected, are are failedandtheresults printed Thecalculated thecalibration previous returns thecalibration coefficients and to calibration instrument keeps memory in the menu.
7. CALIBRATION
CALIBRATION DATE:04102194 FAILED CALIBRATION J O P E R A T O:R M G L O TN r : 6 1 1 5 N RUN 1 P 2
e 4 5 6
: TIME09:21
WBC 6.3 6.3

o.+
6.5 8.2 6.5 6.78 6.5 11.7 4.30 1.16 0.97 FAILED
RBC 5.30 5.35 5.40 5.31 5.28 5.30 5.32 5.34 0.89 a37 0.90 0.88 OK
HGB 16.0 16.1 16.2 16.1 16.1 16.0 16.1 16.1 0.43 0 1.13 1.13 OK
HCT 47.2 47.1 47 46.8 47.5 47.1 47.1 47 0.54 0.21 0.98 1.03 OK
PLT 230 224 230 240 228 235 231.4 221 2.69 4.70 0.90 0.95 OK
MEAN TARGET cv % cHG REJ. OEFF C CURRENT STATUS

Diag.7-03
IMPORTANT
has failed on one or several parametersnone ol When the calibration them are calibrated.
NOTE
to the or fails, Whenthe calibration the userhasthe possibility restart calibration, to function thecalibration through coefficient the of coefficients the enter directly calibration menu, using password. the
(see when calibration thefollowing fails, setup), the lf theprinter notused is section .4,printer 8.1 menuis displayed : SAVED snv=ocoEFF.wec o.gz RBC0,8s HGB0.e5 COEFF.WBG 0.97 RBC 0.90 0.90 R E J E C T .O E F F . ' ; .' ' 1 , 1 6 C
I
I
keysin orderto using O t'" Rejected saved are and coefficients displayed O "no Pressthe ESCAPE to return the main key to checkand to record faultyparameter(s). the menu. calibration 7.5. CALIBRATION COEFFICIENTS whenthey are the coefficients by Calibration be achieved directly changing calibration can known. Movethe cursor function to fl menuis displayed : The following COefflClENTSand pressENTER.
qq
7. CALIBRATION
7.5.1. Changinqcalibration coefficients Movethe cursor function to O \_J
displayed:
B.COEFF. pressENTER. menuis Cnf-f and The following
password requested enterthe function. is to Enter passwotd"123" the one or A specific the pressENTER. following The menuis displayed: in 8.3.2and defined section
for Enter newcoefficients WBCand RBC,thenmovethecursor downto the HGB,HCT, the new PLTpositions enter required coefficients. the and
'1&M,,.,,:;
i,.J; *,==:
l
' tt,,
pressENTER record setup. have to the Whenthe required coefficients beenchanged, 7.5.2.Print coefficients 7.4.1), is possible printthe coefficient menu(section it values. Fromthe COEFFICIENTS to pl Movethe cursor function on enff.ff COEFF. pressENTER. printout the The and of valuesstartsautoia'tically. coefficient COEFFICIENTS : D A T E0 1 / 1 1 / 1 9 9 5 CURRENT WBC :0.97 RBC 0.88 TIME:HH:MM HGB HCT PLT 1.13 1.03 0.95
Diag.7-04
7.5.3.Coefficientlimits corresponds the following to ranges : coefficients Checkthatthe calibration LIMITS Minimum Mean Maximum WBC 0.80 1.00 1.20 RBC 0.65 0.81 0.97 HGB 1.10 1.38 1.66 HCT 0.83 1.04 1.25
PLT 0.86 1.07 1.28
Diag.7-05
lf not,callyourABX DiagnosfibsRepresentative ServiceDepartment.
CONFIGURATION 8. INSTRUMENT
8 . 1 R E S U L T SP T I O N S . O rt 8 . 1 . 1R e p r i n e s u l t s . Printerselection 8.1.4. LIMITS 8.2. CHANGE LABORATORY o 8 . 2 . 1R e s u l t wl i m i t s . . t l 8 . 2 . 2R e s u lh i g h i m i t s. . . . . . . . . . values Print limits flag 8.2.3. and S.2.4.Flagadjustment . . 8 . 3 S P E C T A L N C T T O.N.S. . . . . . . . . . . . . FU 8.3.1. Change operator password 8.3.2. Change 8 . 3 . 3S t a r t uc y c l e . p m 8 . 3 . 4I.n a c t i v eo d e Autocleaning frequency 8.3.5. printout Internalsetup 8.3.6. signal 8.3.7. Cycle audible end ldentification ......... mode 8.3.8. D 8.4. ATEANDTTME 8 . 4 . 1C h a n g e t i m e . or 8 . 4 . 2 . D af1 e m a t 8 . 4 . 3C h a n g e d a t e . 8.5HOSTOPTTONS . 8 . 5 . 1H o s t c o m m u n i c a t i o n . . . . . . . . . . . . . 1 8 . 5 . 2S e n d i m i t s . . . . . . . . . . . r 8 . 5 . 3B a u d a t e. . . . . . . . . . . . . Transmission 8.5.4. MEMORYCARD 8.6.
. , . , . , . .2 . . . . . . . . . . .2. . . ......... 3 .......... 4 . . . . . . .4. . . . . . . . . . . . . .5 . . . . ............. 5 .........5 . . . . . . . .6 . . ......... 6 .......7 ..............7 .............7 ................. 8 .................... I ............... I ............... 8 . . . . . . . . . . . .I. . . . . . . . . . . . . . .I. . . . . . . . . . . . . . . .9 . . . . . . . . . . . . . . .9. . . . 1. . . . . . . . . . . . . 0. . . . . . .1 0 . . . . . .1 0 . . . . .1 0 ............ 10 ...............11
li l i.i.l. 'll ll l l llllllllrr..'.... *'i1'....'..'............
8. INSTRUMENT CONFIGURATION
: options TheABC Vethas operator several - Specific limits. laboratory - Dateandtimeformats. - Result format. - RS232options. - Specialfunctions. needs function through SETUP the to according theoperator These options beconfigurated can movethe cursor the function to Fromthe MAINMENU, of the MAINMENU. f;l and press \__J : menu displayed is ENTER. SETUP The
SETUP HH:MM
OPTIONS 8.1. RESULTS
:it ;ii:i
::tii
: the options to The RESULTS the menuallows operator access following - To reprint results the lastsample memory. in of the - To printoutor notthe RBCand PLTdistribution curves. - To select unittype. the - Toselect printer type the - To select notthe limitprintouts. or to From setupmenu, move cursor thefunction the the O : RESULTS menuis displayed RE$ULT$ HH:MM Reprint results 8.1.1. in movethe cursor function to of the To reprint results the lastsample memory, the of Q re-printed is of The ENTER. results thelastsample automatically RESULTS menu press and numbers, run the sample andsequence identification, withthe dateandtime,the associated (see .2). is possible if flags thehistogramstheirprintout selected 8.1 and Printout 8.1.2. (histograms) WBC,RBCandPLT beprinted when option can out this for Thedistribution curves function move cursorto the menu, is selected. From RESULTS the Q : HISTOGRAM menu displayed is press The ENTER. anO RESULTS pressENTER, the and
Yi:: FFtNrdil$ ,+ H*:Mfrrl i,:/!l J$$
#; ,i;T-;
rH Ii TOGRAM$ 'HOj
., HISTOGRAMS'
: will ENTERresults be printed reprinted and function press and Move cursor therequired to the to withor without histograms according the selection. the
8.1.3. Units 4 whenmoving the accessible unit between different systems has The operator the choice pl The ENTER. UNITS andpressing menu thefunction UruffS to fromthe RESULTS cursor
\__J
: menuis displayed
are The4 different systems : STANDARD WBC RBC PLT HCT HGB MCV MCH MCHC MPV 103/mm3 106/mm3 103/mm3
ol /o
SI
INTER1
INTER2
109/l 1012t1 109/l
109/ 10124 109/
103imm3 106/mm3 103/mm3

o/
fo
vl
mmol/l fl fmol mmolil fl
vl
gll fl
Table B-01
g/dl pm3 pg gldl Lim3
g/dl fl
pg g/dl fl
pg gll fl
unit and in Move cursor frontof the required system pressENTER. the 8.1.4.Printerselection menu. printers a no printout fromthe RESULTS optioncan be selected and Fourdifferent menuis The PRINTER to Movethe cursor the function O pnfrufERand pressENTER. displayed:
printer : Move cursor the required the to - 1 RESERVED : EPSON columns) printer (80 1 - 2 RESERVED STAR printer 2: - 3 RESERVED : SEIKO printer (thermal) 3 - 4 STANDARDCITIZEN matrix) printer (dot : - 5 NONE In to andpressENTER. move cursor thefunction NONE the is or if no printout required, O fromthe displayat the end of by will this lastcase,results haveto be recorded the operator eachanalysis.
Printlimits 8.1.5. laboratory limits")can printed underneath be out in Change Thelaboratory (entered "8.2. limits menu, move cursor function From RESULTS the the to is when option selected. eachresult this menu displayed is : press LIMITS The ENTER. PRINT [s ] and PRTLIMITS HH:MM
>,,",::l,,,YE$
,;:::.::NG::
with to the and Move cursor YESto print results the limits the The* indicates current state. the ENTER. press without limits press the the and Move cursor NOto print results to ENTER. the LIMITS LABORATORY 8.2.CHANGE
IMPORTANT
Limitsare differentaccordingto the species.Theselimits are recordedon the smartcard and can be modifiedby the user (seesection8.2).A confirwhenexitingthe Change Lab.Limitsmenu. mationof the recordis required
with the limits above upper Results exceed laboratory areidentified a flag: H for results the that limit. limit, for results L below lower the is via whenthisoption computer the RSoutput to These limits betransmitted an external can (see validated section 8.5.2). move cursor thefunction to From SETUP menu, the the O : is TER. menu displayed TheLABLIMITS CHGLABLIMITS Ht{:MM Resultlow limits 8.2.1. Movethe cursor function to lil \-J : displayed menuis The LOWLIMITS f-OW LIMITS pressENTER. and ENCHGLABLIMITS press and
> 1 LOWLIMITS 2 HIGH LIMITS
:XX
<xxi ;
;.:::
ffi
(RBC parameter press for ENTER, following menu and the next Move cursor to therequired the : is example) displayed
value. ENTER press ESCkeyto keep current or the the low andpress Enter required value the parameter follow sameprocedure. Press ESC the and the Move cursor thenextrequired to the out. have carried keywhenallmodifications been
Resulthiqh limits 8.2.2. and to movethe cursor function I HIGHLIMITS press menu, Fromthe CHGLABLIMITS 12 \-J ENTER.
low fortheresult limits. Followthe same Drocedure Printlimits and flaq values 8.2.3. as The and ENTER. high lowlimits well and Move cursor function enfruf LIMITS press to the @ : out 8-01) (see are 8.2.4) printed (Diag. section astheflagvalues
LIMITS DATE:23i11/1994 TIME:09:39
LEI : RBC i lffii ICT i ]fl., i Itlt I lltlf, I PLI I PLT Flass:
LOW 3.5
HIGH 10,0 10t4nP 5.80 106ffn1 16.5 g/dl
3,m
11.0
s,0
25.5 xl.5 150
50.8 9l S.5 s.0 390
I Ftn3 Pg atdl 103rrm3
sLh sqfl:
lllcl:
8,0 8.0 8.0
Diag.8-01.
.Diag.8-01shown ABC Vet Spprinted limits out a Flaqadiustment 8.2.4. on move cursor thefunction the From menu the CHGLABLlMlTS, Q : is TER". flagmenu displayed The "ENff-nCSandpress
(SCL menu "ENTER". following The and Move cursor front theflagto beadjusted press in of the : forexemple) displayed is
value. or to valueand press"ENTER" press"ESC" keepthe current Enter flagrequired the key Press "ESC" the the in Move cursor frontof the nextflagandrepete sameprocedure. the menu. to to values havebeenadjusted return the previous whenall required NOTE is sensitivity. higher the triggering Lower the flagvalue, is
(dogbydefault) : are values Thefactory adjustment S C L :3 . 0 0 FUNCTIONS 8.3. SPECIAL to a allow user : the through password These functions accessible special 1-ldentify4users 2 - Change password the mode 3 - Choose STARTUP the frequency 4 - Adjust cleaning the of 5 - Print internal the SETUP theinstrument signal endaudible 6 - SetON/OFF cycle the mode 7 - Choose identification the The to menu, movethe cursor funtion Fromthe SETUP O SpeCfnLandpress"ENTER". in Entertnffisword <123> the one defined or ?> message <PASSWORD is displayed. : menu displayed: is is "ENTER". thepassword correct, SPECIAL lf the section 8.3.2 andpress
S C H: 4 . 6 7 MIC: 4.37
E O S: 2 . 0 0
8.3.1. Change operator (calibration, require operator identification. different Four the functions QC) Some theinstrument of From SPECIAL menu, the modified anytimeby theuser. at identifications be entered and can : is ENTER. CHANGE menu displayed The OP move cursor thefunction the to fJ-'l andpress \____-,,
identificationbechanged pressENTER. to and operator in Movethecursor frontof the required (example operator : 1) for Thefollowing is menu displayed
(4 maximum). Alphabetic identification be can identificationcharacters Enter newoperator the key the the andCT=) keys.Press ENTER to memorize identifientered using G the identifications forthe3 other Repeat sameprocedure the figure. the cation ESCto keep current or if necessary.
8.3.2.Chanqepassword functions suchas : someimportant to is The useof a password mandatory access - Changing calibration factors. the - Accessing technician functions. the - Changing password. the fromthe SPEis movethe cursor lf password <'123>. the change requested, is The original CIALmenuto function Q : menuis displayed pressENTER. PASSWORD The anO
iess,r;r. cnG
CURRHNT:. i characters if 3 Enter newpasswordnecessary,numeric the password displayed. is Thecurrent one. the or keyboard ESCto keep current maximum using numeric the Startupcycle 8.3.3. in out startup rinse anydetergent the to day every attheinstrument is TheSTARTUP cycle used any before whichmust determined be count includes background a cycle The system. STARTUP interferences that to is necessary ensure thereare no extraneous This samplesareanalyzed. of noiseandaffectthe cellcount.lf the results the bacas that may be detected background performs given a 6.3, specifications in section the analyzer the kground countare notwithin ly. P second STARTU cycleautomatical accessed or startup, manually at ThisSTARTUP cyclecanbe runautomaticallyeachinstrument fromthe needs, according theoperator's to the key. configure instrument To using @ the "ENTER". STARTUP The the menu, move cursorto function SfnnfUP andpress the SpECIAL [l \__J : menuis displayed
is "ENTER". When MANUAL setup selected, the and setup press Move cursor therequired to the : startup at will message bedisplayed theinstrument thefollowing
thentne @ Press keyto access MAINmenu the a
is keyif an analysis required.
IMPORTANT
8.3.4.Inactivemode
will NOTINITIATED" be printedout with the analysis "STARTUP A message cycle is not carriedout afterthe startupof the resultswhen the STARTUP instrument.
(auto cycleautomatically mode)after4 hoursof the This modeallows userto run a startup (except menu). is SERVICE which evermenu displayed This occurs instrument inactivity. cycle the menu, move fromtheSPECIAL needs, to according theoperator's Toconfigure instrument the is menu MODE The and tVE cursorto function I trunCf MODE press'ENTER". INACTIVE the |+ \_/ : displayed
>-1AUTO INACTIVFMODE . H H : M M i i , , I M A N U A L "ENTER". newsetup recorded. The is and option press Move cursor therequired to the Autocleani f requencv nq 8.3.5. (see is carried every samples out 50 solution normally involvingcleaner a Anautomatic cleaning frequency cleaning the according to The section 6.6.2). userhasthepossibilty setup automatic menu, From move cursor the run usually initslaboratory. theSPECIAL tothenumber samples of "ENTER". CLEAN. is The FREQ. menu displayed: FREQ. press and tothefunction CLEAN. llJ F . C L E A N R E Q? : CURRENT] E X I T :E S C S A V E :E N T E R
frequency is The cleaning frequency press"ENTER". newautomatic and Enter required the recorded. Internal setupprintout 8.3.6. Move cursor function the on out this Thesetup alluseroptions be printed using function. can of r^-\ (limits configuration) internalsetup and The SETUP press'ENTER". instrument and gJ PRINT is printed out. Cycleend audiblesignal 8.3.7. menu. Move cursor the (BlP) be setupwiththeSPECIAL the to A cycle can endaudible signal *ENTER". audible menu displayed is : The signal function )gUZZeR press and 7
The is and Move cursorto required option press'ENTER". newsetup recorded. the the ldentification mode 8.3.8. : Twoidentification modes available are - USmode:theoperator letters numbers of eachpatient using or must typeintheidentification The results are will out (see 6.5). section Theidentification beprinted withtheresults. STARTUP printed too. out or - Standard This running analysis an series. before a can mode: theoperator enter runnumber and out cycle printed withtheresults. runnumber beincremented each will on Move cursor thefunction the to O
:..:::a:::-::a:.
"ENTER". following The menu displayed: is and 'OMODE press

iir*
.ii]#f :.-.:.:.:.:.rill
rDMODEI
l-tflMM - 'l 'i
i,ii
"ENTER". new"lD MODE" recorded. The is and option press Move cursorto required the the
ANDTIME 8.4. DATE From specifications. the SETUP to according the country Dateand time can be changed to menu, move cursor thefunction the O : menuis displayed DATET|ff*e l. i rlH:MM
8.4.1.Changetime
"ENTER". DATE TIME The TIMEandpress DATE
Move cursorto function the the Q
menu displayed: is press "ENTER". CHANGE TIME The anO
The HH.MM press"ENTER". newtimeis recorded. and Enter required timein theformat the 8.4.2.Dateformat (the FORMAT current to move cursor thefunction DATE the TIMEmenu, From DATE the O FORMAT menuis displayed: The and is setting displayed) press"ENTER". DATE
4 different can be used: dateformats
MM.DD,YY DD.MM.YY YY.MM,DD YY.DD.MM
'ENTER'. is Thenewdateformat and selection press in Movethecursor frontof the required recorded. 8.4.3.Chanqedate DATE andpress to movethecursor the function 1CHANGE Fromthe DATE TIMEmenu, Is 'ENTER". CHGDATE menuis displayed: The
"ENTER". new The in recalled themenu andpress to Enter newdateaccording theformat the dateis recorded.
OPTIONS 8.5.HOST laboratory via computer theRS232 data is of TheABG Ver capable transmitting to an external plug to to computer beconnected theanalyzer, oneendofthe interface.youhave external lf an Plug vendor) theexternal into computer. theother (provided yourcomputer by computer cable as on receptacle therearoftheinstrument shown the at into cable thecable endof thecomputer 2.10. section NOTE the specifications, to computer TheABC Vethasto be setupaccording the external computer specialist only. by have functions to beused yourlaboratory following port : of Thedefault setup theserial areasfollow :8 1 - Byte number 2-Parity:none 3 - Stopbyte: 1 4-Xon/Xoff:none and press to Fromthe SETUPmenu,movethe cursor the function O HOSTOPTIONS \__J menuis displayed: "ENTER". HOSTOPTIONS The
"ENTER" access different settings. to the and option press in Move cursor frontoftherequired the "ENTER" record newsetup. The the and to setting press in of Move cursor front therequired the : are settings asfollow different options their and . 8.5.1Hostcommunication ( 1 - F O R M A T lA B X ) 2 - FORMAT2 3 - STANDARD 4 - TR OFF(transmission OFF) 8.5.2. Sendlimits 1-YES 2-NO 8.5.3.Baud rate 1-300 2- 1240 3 -2400 4 - 4800 5 - 9600
Transmission 8.5.4. and move cursor thefunction TRANSMISSION to menu, the Fromthe HOST OPTTONS fTl \__J via laboratory computer the RS pressENTER. lastresults transmitted theexternal to are The 232.
8.6.MEMORYCARD lntroduction 8.6.1. with Thismenuis available if theABG Velis equipped chipcardreader. only functions : program the TheMEMORY contains sixfollowing function. the to 1 - MEMO allows operator enable/disable memory : the identification stored is the of 2 - PRINT LIST: thepurpose thissubmenu to print listofthepatient card. on thememory only. to oneresult allows operator print the ONE: thissubmenu 3 - REPRINT recorded the on to out the allows operator print alltheresults 4 - REPRINT : thissubmenu ALL memory card. fromXXXto YYY. 5 - PRTFROMTO : printout results card. recorded the memory on alltheresults 6 - CLEAR CARD: erase to the From SETUP menumove cursor thefunction the O : is Thememory menu displayed MEMOCARD pressENTER. and
' * ''l Yfi,HF,-E?*l;i :rii ri
HH:MM:ffi
8.6.2.Memoon/off Fromthe Insert card into the slot in the upperleft cornerof the analyzer. the MEMORY function move cursorto MEMOCARD menu, the [l \.J menuis displayed : The ftff ftfOandpressENTER. following
on card.The the backup the memory or Thisfunction the allows userto enable disable result . indicates current function, move the To function. activate MEMO the of state the memory the - ON andpress move cursor 2 - OFFandpressENTER. lhe to To ENTER. disable cursor 1 to
IMPORTANT
modeturns to US mode.lt As soon as the MEMOis ON,the identification is mandatoryto enter the identificationbeforerunning the analysis.
Runninqthe samples 8.5.3. The the Plunge probeintothe sample tubeand press sampling to startthe analysis. bar the following : menuis displayed
Pnf tp *:,t . CURRENT :

Then, analyzer ENTER. if the checks a MEMO CARD Enter patient identification press and the is present. - lf the Cardhasnotbeeninserted, following message : the appears
::::::::: :: ,::=, . l':::i.: i:::::.::=
$t0i'-.....= ;tt.!,.1!LQti = S i hlTEA:l
ENTER. Insert cardandpress the - lf thecardis notthe MEMORY thefollowing message warns operator one, the
Replace cardby a memory andpressENTER. one the - lf the cardhasbeenintroduced a wrongway in

. tl:l
foreward on the uppersideandpressENit and Remove cardand insert withthe arrow the TER. Whenthecardis full :
ENTER. Insert newoneandpress a are on card,as wellas thepatient When analysis completed results stored thesmart is the the identification. CAUTION : I fne graphic resultsare not recordedon the card.
When oneis full,theoperator musteither replace the this 60 results be stored thecard. can on of fromthecurrent or all cardby a newoneto carry thebackup the results clear the results on card(see8.6.7). whichcorresponds the indexof the results to The results storedundera backupnumber are recorded the card. on NOTE if Results notbe recorded the MEMOis OFF. can
8.6.4.Print list identifications recorded to The PRINT the LISToption allows operator printoutallthe patient (column number as on the card,the time and the date of the analysis well as the backup MEMO). ro move cursor menu, the From MEMOCARD the O ENTER. PRINT LIST andpress
vFri-lsT :
Example :
l:<ON>
MEMO 1 2 3 4 5
DATE 10t23t1995 10t23t1995 10t23t1995 10t26t1995 10t26t1995
TIME '16:39 17:18 17:26 17:33 17:36
NAME 123 951 1235 784 895
Diag.8-02
8.6.5.Reprintone is with This number displayed results number. backup involves backup the Reprinting result one identification. underneath patient the
t:
ii::.
t::;1
the ONE and pressENTER. to movethe cursor f 3-'l PRINT menu, Fromthe MEMOCARD \__J : following menuis displayed
i,i 'GUHg#' Wt
me
rVE:ESC
i ErutEn
The are out to of Enter backup number the results be printed and pressENTER. results the (on the printed withthe backup below time). number the right, out in on stored thecardis displayed CURRENT]. number results of [ NOTE I tne totat Reprintall 8.6.6. Check quantity the stored thecard. on alltheresults to Thisfunction allows operator reprint the movethe cursor to menu, ALL.Fromthe MEMOCARD of paper running REPRINT a before without out The are ENTER. results printed in theusualformat neentNTALLandpress [! graphs. (the pressESCuntilit hasbeendetected the program by lf the printout to be interrupted has printout). program checks keysonlyat the endof a result the
8.6.7.Print from to on recorded thecard' only to the allows operator printout oneparlof the results Thisfunction TO movethe cursor,oo pRT FROM andpressENTER. menu, Fromthe MEMOCARD
Move cursor,o the O
: is submenu displayed :the BEGIN ENTER and BEGIN press
out to of number thefirstresult be printed andpressENTER' Typein the backup to Movethecursor O : is :the ENDsubmenu displayed ENDandpressENTER
''' ,UffrYf*'E?c' i;:.i': EU*o'

'-
Movethe out to of number the lastresult be printed and pressENTER' Typein the backup out are The results printed in the usual and RESULTS pressENTER. ro cursor O PRINT the without graphs. format 8.6.8.Clearcard to the allows operator eraseall the on can not be erased its own.Thisfunction One result movethe cursor,oO CLEAR menu, on recorded the card.Fromthe MEMOCARD resutts Confirm the to is A ENTER. message displayed warntheuserabout erasing. andpress CARD ESC' or withENTER cancelwith for be re-used to is are The results erasedandthe backupnumber updated 0. The cardcan newresults.
AND TROUBLESHOOTING 9. MAINTENANCE
taaa
AND 9. MAINTENANCE TROUBLESHOOTING

9.1.MAINTENANCE SERVICE AND 9.1.1. Overview results a well-maintained is to and factors contributing accurate reliable Oneof the principle properly. functioning The is to instrument. maintenancerequired keepyourinstrument Routine maintenance automated to a minimum. Thissection and ABC Vetis designed keepthis to procedures. maintenance describes dailyandperiodic the Dailymaintenance 9.1.2. performance yourABG procedures required dailyto maintain optimum of These are cleaning Vet
IMPORTANT
cleaningproceduresmay Failureto performany of these recommended result in decreasedreliabilityof your system.
cycles 9.1.2.1. andstandby Startup cyclemustbe performed. can be performed This At the beginning eachday,a startup of involvement manually pressing G'TD fey. or by tne without operator's automatically the goesinto"STANDBY" theend key.Theinstrument at At theendof eachday,press @ the (instrument in Leave instrument thismode the 1 overnight ofthecycle. Thiscycle takes minute. reagent visible thechambers. is in hasto beswitched inthismode), cleaner blue off 9.1.2.2. Automatic cleaning programmed the after by A cleaning automatically the number samples of cycleis activated to workload, section can see 8.3.4for operator. cyclefrequency be adjusted the laboratory The This fromtheservice menu(see theAUTOMATIC CLEANING SETUP. onecanbe rundirectly section 9.1.3.9). general cleaning 9.1.2.3. lnstrument with of Usewater anda dropof liquid Ingeneral, intrument to becleaned a wetpiece cloth. has Never solvant abrasive materials. of use or soapif necessary clean outside theinstrument. to the Disconnect instrument themain from electrical Wipe anytrace blood soonas possible. as of off is surethe instrument clean anddrybefore intervention make and supply before cleaning any reconnection. 9.1.2.4. filter Air nextto thepower in to at supply order An airfilteris placed inside instrument, therearbottom the or by retain dust.Thisfilterwillbe cleaned replaced an ABX DiagnosticsRepresentative the maintenance. Technician during preventive the Service only functions Service 9.1.3. These for functions available the userto cleanandcheckhis instrument. are Several service Move cursor the Function SERVICE the to functions accessible theMainmenu. from are l.T-) \-J : menu displayed is The andvalidate. SERVICE w,sttw - 4 W Ii
wj,:
1 hi
! . Wri: 1
,ffi
; - j

Backflush 9.1.3.1. Movethe in aperture caseof blockages. Thiscycleallows userto cleanthe instrument the is out ENTER. backflush The cycle carried and cursor thefunction BACKFLUSH press to O from are thatthe liquids aspirated the WBCand Check approximately. and lasts22 seconds backintothe thatthe liquids alsopushed are Check the RBCchambers through apertures. cleaning Perform concentrated a maybe blocked. the lf chambers. it is notthecase, aperture 9.1.3.4. procedure described section in as Drainchambers 9.1.3.2. properly to maintain and are thatthe chambers drained Thiscycleallows userto check the This it flushes liquid of the instrument. cycle out the manifold as somepartsof the hydraulic and CHAMBERS to Move tasts seconds 25 approximately. thecursor thefunction DRAIN [tl \___J pressENTER. - Checkthatthe wastechamber drained properly. not,checkthatthe wastelinefromthe lf is is or at chamber thewasteconnector the rearof the instrument notcrimped blocked. to - Checkthatthe WBCand RBCchambers drained properly. not,checkthatthe drain lf are or are chamber notcrimped blocked. to tubesgoing thewaste - Check properly. not,check tubesare lf thatthefollowing is chamber drained thatthe mixing : notcrimped blocked or - Check tubebetween bottom the mixing to chamber thetop of theWBCchamber. of the the - Check tubebetween topof theWBCchamber thevalve<3>. to the the - Check tubebetween valve chamber. <3>andthe waste the the Primereagents 9.1.3.3. containers one whenreplacing or all reagent the Thiscycleallows userto prime reagents the (orthepack). to Move cursor thefunction the S priming cycle: A - Bottlesand containersset - All reagents - Diluent priming - Lysepriming - Cleaner priming thattheyareclearof air bubbles. Check lines andpump. reagent Visually inspect B - ReagentPack given the and PACK follow instructions bytheLCDin order Select function : CHATGE the Q carried will cycle be automatically installed priming a Oncethe newPACK to install pack. the that linesandpump.Check theyareclearof airbubbles. reagent inspect out.Visually Run ENTER. therequired enfnfE andpress

9.1.3.4. Concentrated cleaning This of Thisprocedure cleaning the RBCand WBCapertures. cyclelasts3 allows strong a interventionfillthe with to chambers 1:5diluted the minutes approximately involves operator and bleach solution. :4 waterto parthigh 1 as deionized is The1:5bleach solution prepared follow parts NOTE: quality, - available (1 hypochlorite chloride). sodium bleach 0 -15% fragance-free ENTER. following The and CLEANING press Move cursor thefunction CONCENTRATED the to Q menuis displayed :
menuis displayed : anykey.Thefollowing coverdoorandpress Openthe instrument lN SOLIJTIONI POUR3MLOF BLEACH PRESS KEYTO CONTINUE;;". , A The into a pour chamber press key. and solution themixing Using 5mlsyringe, 3mlof bleach a : nextmenuis displayed IN4qBC CHAMBER SOLUTION POUR 3MLOF B-IEAOH ' ... PHESS KEYTO.CONTINUE A and pressa key.The next menuis inside the RBCchamber Pour3ml of bleach solution displayed:
menuis displayed : Thefollowing anykey. coverdoorandpress Close instrument the
Every10 seconds staris cleared The a off. of at Stars displayed the beginning thecycle. are to fromthe mixing chamber procedure of solution transfer thebleach involves cycles, different which cleaning theapertuof rinsing, allow perfect a aspiration, backflush, theWBCchamber, analysis begin. can sample is res.After thisprocedure completed, Transfer 9.1.3.5. chamber the WBCchamber. to Usinga fromthe MIXING It is possible check transfer the to Movethecursor thefunction chamber. to pour2mlof diluent the mixing into Smlsyringe, O menuis displayed the cycle starts: and The TRANSFER pressENTER. following and
P, in fromthe mixing chamber to for is check a blockage the lines lf thetransfer notcompleted, to fromthe WBCchamber the valve<3>,andfromthe valve<3>to the the WBCchamber, pressure/vacuum syringe.

checks Mechanic 9.1.3.6. Move cursor thefunction to the O \__J : displayed is menu The ENTER. MECHANIC and MECHANIC press
of detection the allowthe userto checkthe correct A - Sensoroperations: Thisfunction and CHECXSENSORS press to Movethe cursor the function motorhomepositions. Q : is menu displayed SENSORS The ENTER. CHECK
::1
needle - NEEDLE the front : SENSOR openthe instrument doorand pushupward sampling by : to on detection thedisplay0 turns 1 followed 10stars. thecorrect to support thetop.Check - CARRIAGE movethe sampling needle its upperposition, in : SENSOR withthe sampling to : on detection thedisplay 0 turns the Check correct handsideposition. on carriage theright 1 followed 10 stars. by
Press any keyto exitthefunction. rueeOf-E and U/D to : B - Needle anddownoperationMovethe cursor the function up Q and has the translation, movement to be smooth pressENTER. the observe needle Closely regular. UR NEEDLE andpress to : teft/right operationMovethe cursor the function C - Needle O and has the translation, movement to be smooth regular. the ENTER. observe needle Closely and SYRINGE pressENTER. to the : D - Liquid syringe Move cursor thefunction LIQUID O andregular. has the translation, movement to be smooth the Closely observe syringe SYRINGE to : syringe Movethe cursor the function E - pressure/vacuum fll eneSSURE has the translation, movement to be smooth the observe syringe Closely andpressENTER. andregular. observe Closely and to : the F - Valves Move cursor thefunction VALVES pressENTER. O and has the thevalveoperations, movement to be straight regular.

for readability. the : can Move contrast be adjusted a better G - Change contrast Thedisplay cursor thefunction to O is displayed : The ENTER. CHGCONTRAST menu and CHGCONTRAST press
Press + keyof the numeric keyboard increase contrast, press - keyto reduce to the or the the pressENTER validthesetup. is Whenthe bestcontrast obtained, to thecontrast. NOTE I The contrast fromany othermenuby pressing menucan be accessed adjustment
ontn* simultaneously @
"nd O
"yr.
piston the upper position thedilution in H - Park: Raises vacuum/waste syringe and block the position. syringes the lower in 9.1.3.7. Gyclefunctions run Fromthe Thesefunctions allowthe userto checkthe cyclenumbers by its instrument. The to CVCIESand pressENTER. CYSERVICE menu, movethe cursor the function @ : CLESmenuis displayed
: ,3;f$$lli:i l;'t'r: : ,,,

: Thefollowing STANDBY CBCs. and cycles be reviewedSTARTUP, can functions 9.1.3.8. Technician to on basis. Thisfunctions the engineer checkthe instrument a technical allows fieldservice password. Thesefunctions be accessed withthe useof a specific only can 9.1.3.9. Automatic cleaning Movethe cursor function to Q press"ENTER". anO
(seesection workload 8.3.4). Thecyclefrequency be ajdusted the laboratory to can

9.2.TROUBLESHOOTING 9.2.1. Overview if : require troubleshooting YourABC Vetmayoccasionally - System are operations faultY. - Thebackground high. is count unacceptably - Your (e.9., high results suspicious consistently are or values out-of-range, patient are control methods). results manual by to RBCcounts inability verify or - Precision poor. is - Calibration drifting. is see mentioned thefollowing in discussions, partsof theanalyzer to NOTE I lf needed, locate in 9.4. valveis listed section of 5. of thediagrams thesection Thefunction eachliquid procedure 9.2.2.ldentification malfunction: is the of session to identify source thesystem Thefirststepin anytroubleshooting out precision, calibration. Thesestepsshould carried in be or reagents, system operations, : below sequence described as . 9.2.2.1Systemoperations in 4. as operation described section lf the ABCVet the Press the keyandobserve instrument procedure. operations are lf properly, with continue the identification appears be operating to procedures appropriate troubleshooting and of faulty, identify source the malfunction initiate the (seesection 9.2.4). Reagents 9.2.2.2. your or values out-of-range,yourpatient are is lf yourbackground count unacceptable, control your Replace can or reagent deterioration contamination be suspected. results suspicious, are procedure Obtain in 9.1.3). described seciion cleaning the reagents perform concentrated and lf samples. reassay or controls patient 6.3),andif appropriate, count(seesection a background (or results are but countis acceptable, controlvalues stillout-of-range patient thebackground procedure. replacing reagents performing lf and with continue identification arestillsuspicious), count,call your ABX Diagnosfics does not correctthe background cleaning concentrated Representative SeruiceDepartment. 9.2.2.3. Precision (%CV). of the andcalculate coefficient variation 5 sample to 10times Analyze freshpatient a by of deviation the measurements the the by %CVis calculated dividing standard by this multiplying result 100. meanand
'X
SD=
r(x-xi
where: X = mean n | - individual value

V.
deviation SD= standard Il = # of observations Parameters wBc RBC HGB HCT PLT %CV <2.5h <2.0h <1.7"/" <2.0h <5.0%
: Thefollowing should obtained be CVs
procedure precision acceptable. the precision any if is lf of Proceed with the identification paramete(s) initiate parameter notwithin identify out-of-range the and is thesespecifications, (see procedures section 9.2.3). appropriate troubleshooting 9.2.2.4. Galibration properly, freshuncontaminated reagents beingused, are lf thesystem appears be operating to your Calibrate the calibration. is the andtheprecision within specifications, ABCVetmayneed 7. in instrument described section as parameters Troubleshootinq 9.2.3. precision a parameter is wheneverthe of should performed be Theprocedures below described result incorrect suspicious. is or or noted notwithin specifications above, a parameter the WBCand HGB 9.2.3.1. Press the are or if Perform following bothyourWBCandHGBresults incorrect suspicious. the listed in of below theorder specified. operations theanalyzer keyandclosely the observe specific procedure(s). troubleshooting appropriate ldentify malfunction(s) initiate and the probe is cycle, thesample between edgeof the the dilution the Sampleprobe : During initial (Diag. close thebottom to 9-01)? mixing of chamber thecenter thechamber, and
Diag.9-01
position themixing probe notin itscorrect ABX in chamber, callyour is lf it is,continue. sample lf DiagnosticsRepresentative SeruiceDepartment. ls in dispenser? theplunger thediluent of : Diluent dispenser Doyouseebubbles thediluent (Diag. sample analysis 9-02)? during moving anddown smoothly dispenser up
o o o
Diag.9-02
properly, is lf continue. yousee dispenser operating lf youdo notseebubbles thediluent and pointD properly, refer section to 9.1.3.6, is dispenser not operating bubbles, if the diluent or Liquid for actions. syringe appropriate
cl/cl

from dilution transferred the Transfer initialdilutionto WBCchamber: ls all of the initial of (Diag. 9-03)? mixing chamber theWBCchamber to
Diag.9-03 if is Liquid transfer the initial dilution not completely lf it is, continue. 9.1.3.5. See section to from chamber theWBCchamber. transferred themixing moving anddownsmoothly? up : Transfer lyseto WBGchamber ls thelysepumpplunger of (Diag. 9-04)? Canyouseeair bubbles
Diag.9-04
properly, no bubbles seen(thussystemoperations all are and lf the lysepumpis operating . ServiceDepartment call appear beacceptable), yourABX DiagnosfibsRepresentative to in bottle. Change reagent the bottle of lf bubbles seen, check level MINILYSE thereagent the are (orpack) primethe Callyour reagentlines(seesection2.13). ABXDiagnosticsRepre*ntative and the if Departmenf thisdoesnotcorrect WBCand HGBresults. Seruice

WBC 9.2.3.2. is or if Check following onlytheWBCcount incorrect suspicious. the procedure peformed cleaning cleaningprocedure: Was the concentrated Goncentrated lf the (see reagents)?not,perform procedure section 9.2.2.2, partof theidentification earlier as in 9'1.3.4. procedure described section as cleaning concentrated procedure (see earlieras part of the identification Calibration: Was the systemcalibrated afterthe concentrated WBCcountis stillerroneous section 9.2.2.4\.lfit was not,and the 7. in as the procedure performed, calibrate instrument described section Continue was cleaning if : troubleshooting - Thisdoesnotcorrect WBCresults. the - Theinstrument already procedure. as beencalibrated partof the identification has - Earlier procedure failed. the the attemps calibrate WBCduring identification to valve <6>. of the Analyze sample observe operation theliquid a and <6> valve lf cycle? theliquid during analysis <6> and valve opening closing Valve <6>: ls liquid call the lf thisdoesnotcorrect WBCcount, your the replace valve. is notopening closing, and SeruiceDepartment. ABX Diagnostics Representative Representative *ruice Department. properly, ABX Diagnostics callyour lfthevalveisoperating HGB 9.2.3.3. or are if Check following onlytheHGBresults incorrect suspicious. the performed? it was not,perform the lf cleaning cleaning: Wastheconcentrated Concentrated ifthis .3.4. troubleshooting doesnot Continue in 9.1 cleaning described section as concentrated correct HGBresult. the procedure? HGB lf as earlier partof the identification calibrated Calibration: Wasthe system 7. procedure, in it calibrate as described section wasnotcalibrated partof the identification as press @ keyandclosely observe failed, the have lf allattempts calibrate parameter this to ldentiffthe malfunction(s) in specified. listed below theorder of thespecific operations theanalyzer procedure(s). troubleshooting andinitiate appropriate call to still lf uponcloseinspection operations appear be acceptable, yourABX Diagnostics all Department. Representative Seruice Can plunger moving anddownsmoothly? youseeairbubbles? up Lysepump : ls thelysepump lf continue. lysepumpis properly bubbles notobserved, are and lf the lysepumpis operating Point fortroubleshooting D 9.1 see properly bubbles observed, section .3.6. are or notoperating procedure. power on (seeDiag. lf 9-05)? it is is whenthesystem HGBLED: ls the HGBLEDilluminated yourABX DiagnosfibsRepresentative call are allsystem operations acceptable, andtherefore poweris on,callyour whenthesystem Departmenl.lf theHGBLEDis notilluminated Seruice SeruiceDepartment. ABX Diagnostics Representative
Diag.9-05 9.2.3.4. RBC, HCTandPLT all to if and Check following RBC,HCT, PLTresults appear be erroneous. the performed earlier part as cleaning Concentrated cleaningprocedure: Wastheconcentrated perform concentrated (see reagents)? not, lf procedure section the 9.2.2.2, of the identification Thenpress (S) keyandclosely obprocedure tne in 9.1.3.4. cleaning described section ldentify listedbelowin the orderspecified. the of servethe specific operations the analyzer procedure(s).uponcloseinspection lf all troubleshooting malfunction(s) initiate appropriate and Service call to operations appear be acceptable, yourABX DiagnosticsRepresentative still Department. is moving anddown? lf the12pl up syringe operating syringe Sampling syringe: ls thesample properly is during lf syringe notoperating properly continue. thesampling analysis, during sample ServiceDepartment. sample analysis, callyourABX DiagnosticsRepresentative (Diag. lstheplunger 2)? of in dispenser Diluent : dispenser Doyouseeanybubbles thediluent lf are sample during analysis? noairbubbles moving anddownsmoothly up thediluent dispenser properly, plunger operating lf are orthe is continue. bubbles seen, seen, thediluent dispenser and pointD for properly, section 9.1.3.6. plunger the diluent see is dispenser not operating of troubleshooting actions. in during initial the chamber of Mixingchamber: ls therea stream air bubbles the mixing and the centerof the the dilution cycle?ls the sampleprobebetween edgeof the chamber (seeDiag. section 1, 9.2.3.1). chamber, close thebottom to in lf thereis a streamof bubbles, and the sampleprobeis in its properposition the mixing closeto thebottom) of (between edgeof thechamber thecenter thechamber, and the chamber (seeRBCchamber of lf below). thereis no stream the continue observing RBCchamber by of dilution cycle, check operation theliquid the the during initial in chamber bubbles themixing valve <3>.

lf the replace valve. thisdoesnotcorrect the and lf theliquid valve<3>is notopening closing, lf Department. thevalveisoperating Seruice problem, Representative ABX Diagnostics callyour properly, y ourABX Diagn ostics RepresentativeService Department. call (between edgeof the position themixing in the chamber probe notin itsproper lf thesample is call your ABX Diagnosfi?s closeto the bottom) and the centerof the chamber, chamber Representative ServiceDepartment. of probe and the between edgeof the chamber the center the RBCchamber ls the sample : (seeDiag. ? 9-06) chamber, close thebottom to
Diag.9-06 (between edge thechamber position theRBCchamber the of in probe in itsproper is lf thesample operations appearto and system closeto the bottom), therefore andthecenter thechamber, of ServiceDepartment. beacceptable, yourABX Diagnosfics Representative call (betwepn edgeof the position the RBCchamber in the probe notin itsproper is lf thesample closeto the bottom)call your ABX Diagnosfibs chamber and the centerof the chamber, rtme nt. ve Representati &ru ice Depa RBC 9.2.3.5. Analyse a is or if Perform following actions onlythe RBCparameter erroneous suspicious. the listed in specified. below theorder of operation theanalyzer the normal control obserue specific and procedure(s). lf uponclose troubleshooting appropriate and ldentify malfunction(s) initiate the Representative call be still inspection alloperations appearto acceptable, yourABX Diagnostics SeruiceDepartment. <6> lf valve analysis cycle? theliquid during <6> and valve opening closing Valve <6>: ls liquid properly, ABX is callyour lf properly, replace valve. thevalve stillnotoperating the is notoperating properly, the lf continue Departmenf. thevalveis operating Representative Seruice Diagnostics procedure.

moving and down? lf the 12plsyringe not is up syringe Samplingsyringe: ls the sample point for troubleshooting 9.1.3.6 D acproperly see analysis, section during sample operating properly during sample analysis, continue procedure. the is tions. thesampling lf syringe operating (Diag. in dispenser 9-02)?ls the Diluentdispenser: Do you see any bubbles the diluent plunger thediluent lf smoothly during sample analysis?noair moving anddown up dispenser of plunger operating properly, is lf continue. bubbles dispenser bubbles seen, thediluent are and properly, section is see 9.1.3.6 dispenser notoperating of areseen, the plunger thediluent or point fortroubleshooting D actions. <8>and <11>opening closing valves and during analysis Valves<8>and <11>:Are liquid lf and replace valves. this <8> are the lf valves and<11> notopening closing, cycle? theliquid Representative ABX Diagnostics Department. Seruice callyour doesnotcorrect RBCcount, the properly, your ABX DiagnosticsRepresentative Service lf the valvesare operating call Department. 9.2.3.6. HCT is or if Check following onlytheHCTresult incorrect suspicious. the procedure? HCT lf as earlier partof the identification calibrated Calibration: Wasthe system procedure, it in 7. calibrate as described section was notcalibrated partof the identification as Departmenf thisdoesnotcorrect HCT if Seruice the Representative ABX Diagnostics Callyour result. 9.2.3.7. PLT is Review if or Perform following actions onlythe RBCparameter erroneous suspicious. the patient results. your lf Sampleflags : Weretherefrequent flagsnextto the PLTresults? therewere,replace $ procedure it wasnotalready if doneas partof cleaning reagents perform concentrated the and procedure section (see reagents). youdo notobserve flagson lf 9.2.2.2 the identification $ patient samples, continue. procedure? PLT lf as earlier partof the identification calibrated Calibration: Wasthe system procedure, 7. calibrate as described section it in was notcalibrated partof the identification as SeruiceDepartmentif doesnotcorrect PLT this the Callyour ABX DiagnosticsRepresentative probe is probe: During initial between edgeof the cycle, the sample the dilution Sample the (Diag.9-01)?lf it is, closeto the bottom mixing and the centerof the chamber, chamber in chamber, yourABX call continue. sampleprobeis not in its correctposition the mixing lf Diagnostics Representative SeruiceDepartment. Troubleshooting 9.2.4. svstemoperations whenever should performed be system operations Theprocedures on described the nextpages and the troubleshooting are faulty.ldentify sourceof the malfunction initiate appropriate the procedure. 'SERVICE" in to menuis available the ABC Vetprogtam helpthe userin the A This menu and operations. SERVICE can transfers mechanic troubleshooting hydraulic of the havebeenpassed. whenthefollowing checks be accessed

Power 9.2.4.1. the of of because a lack powerto ABC a to if followingyouareunable analyze sample Checkthe lf into cordplugged thewalloutlet? it is, ls is,continue. thepower power on? switch lf it Vet.lsthe continue. call lf lf in ls therecurrent thewalloutlet? thereis,continue. thereis no current, yourmaintenance department. the to beenunable identify lf theyare,andyouaretherefore Arethefusesstillfunctionning? lf SeruiceDepartment. yourABX DiagnosticsRepresentative failure,call of source the power 2'7'2. in replace themas described section or are thefuses defective blown, Display 9.2.4.2. remains dark, occurs thedisplay and alarm on, is Whenthe instrument switched if an audible properly fitted? : the check followingArebothconnectors - Check flatcable (seeDiag. 9-07)' on connection thedisplay the - Check flatcable (seeDiag. 9-07). board theelectronic on connection the (and failure of the to identify source thedisplay been unable therefore lf theyare,andyouhave the keys access to undm the press simultaneously O occured), no audible alarm point G. in 9.1.3.6 described section the Follow procedure menu. adjustment display Department' Service Representative remains dark,callyourABX Diagnostics lf thedisplay
Diag.9-07
Motors 9.2.4.3. mayshow the occurs, display a or on Afterhaving switched the instrument whenever problem messages These initialization. in a concerningfailure oneofthe motor someerrormessages : and on for appears a fewseconds thedisplay areas follow -"NEEDLE ERROR'' MOTOR -'TRANSFER ERROR" MOTOR -'LIOUID ERROR" MOTOR SYRINGE -,PRESSURE ERROR" MOTOR SYRINGE lf (seeDiag. 9-08)? theyare, board properly fitted the electronic on connectors Arethe motor failure, yourABX call of the to beenunable identify source the motor and you havetherefore SeruiceDepartment. Diagnostics Representative
tk,
lnEuL
o
Diag.9-08
:61-r
6\ 6\-1
t3t ||
/\ll I \-/11--I
dLr!lJr-r^fl-=
ilI--?|'l
/ t
f-----1
H
&r 9 J j "
4 - Needle motor 5 - Fanmotor
motor 1 - Pressure syringe motor 2 - Liquid syringe motor 3 - Transfer
it menu to the havebeenpassed, is nowpossible access SERVICE When STARTUP checks the partsas described in and of in orderto checkthe operation the hydraulics the mechanical section 9.1.3. MESSAGES 9.3.ERROR intervention. Follow insthe the and can Someerrormessages be displayed require operator lf be given eachmessage referto the specified section. the problem cannot for and tructions SeruiceDepartment. ABX DiagnosticsRepresentative solved, callyour : MOTOR" Thismessage bedisplayed theinstruat can A - "ERROR PRESSURE : SYRINGE syringe motor. Checkthat the motoris on mentstartup. showsa malfunction the pressure lt properly 9-0 board(seeDiag. 8). lf it is,callyourABX Diagnosft'cs on connected theelectronic Representative SeruiceDepartment. : at MOTOR" Thismessage bedisplayed theinstrument can B - "ERROR LIQUID : SYRINGE motor. Checkthat the motoris properly on lt startup. showsa malfunction the liquidsyringe board(see Diag.9-08).lf it is, call your ABX Diagnosfics connected the electronic on Representative $eruiceDepartment. at can MOTOR": This message be displayed the instrument C - "ERROR: TRANSFER is motor. connected Check themotor properly that on lt startup. shows malfunction thetransfer a on the electronic board(seeDiag.9-08).lf it is, callyourABX DiagnosfibsRepresentative SeruiceDepartment. lt startup. : can at MOTOR" Thismessage be displayed the instrument D - "ERROR NEEDLE : motor. Check the motoris properly that connected the on on showsa malfunction the needle Service board(seeDiag.9-08).lf it is, call yourABX Diagnosfics Representative electronic Department. when operator some appears the attemps print to :Thismessage E - "PRINT PROGRESS" lN printout. dataduring result a is whenthe printerrunsout of F - "ERROR: OUT OF PAPER": This message displayed paper. - PressESCto clearoff the message the and to complete cycle,the last resultcan be repaper(seesection 2.11)or, printed a afierinstalling newrollof - install new rollof paperas described section in 2.11and pressENTER. resultwill be the a printed automatically out.

is whenthe printer off line. is OFFLINE":This message displayed G -'ERROR : PRINTER in lamp, according thetypeof theprinter use) to lamp(orselect of Check status theonline the the whenthelineis onto printautomatically result. ENTER 2.11 in as described section . Press is whenthe printer off or not is : H - "ERROR: NO PRINTER" This message displayed in 8.1.4to set up described section the or Reconnect printer follow procedure the connected. printer. without the instrument is whena printout is : NOTSELECTED" Thismessage displayed t - "ERROR: PRINTER in 8.1 described section .4 to Follow procedure the has requested no printer beenselected. and selecta printer. in is J - "ERROR: BAD DATE! TRYAGAIN": Thismessage displayed the datechange with is (seesection whenthe newdateentered incompatible the dateformat 8.4.2) function to the dateor change dateformataccording the a 8.4.3.Re-enter correct setupin section procedure in 8.4.3. described section in time is K - "ERROR: BAD TIME ! TRYAGAIN": This message displayed the change Re-enter format HH.MM. : whenthenewtimehasnottherequired (see 8.4.1) function section time. the correct requests a whenthe operator is BAR" : Thismessage displayed L - "PRESS SAMPLING probe) andthe sampling the bar (START or sampling behind sampling key sample analysis the key position). firstpress theSTART willlower The on (upper position probe in itsstanby is presswillstartthe analysis the is probe message displayed, second andthe above sampling cycle. whenever ESCkey is the is BY M - 'CYCLEABORTED USER": Thismessage displayed "CYCLE is ABORTED?" displayed. message A pressed cycle. confirmation during hydraulic an out cycle key,an initialisation is carried to the by is lf theabortion confirmed pressing ENTER in reinitialize motors theirhomeposition. the
IMPORTANT
cycle to After abortingan hydrauliccycle,it is necessary run a STARTUP instrumentbeforeany sampleanalysis. to rinse the
in is : XXX,MAXI : XXX" :this message displayed the following N - "BAD VALUE...MINI : occasions : - Whenthe sample number above 6.5'1). 9999(seesection is run - When target (see 7.2.4) oul of the are in values entered theAUTOCALIBRATION section the limits. - When number sample (see 7.2.5) outof is in selected theAUTOCALIBRATION section of the (3 thelimits to 11). - Whenthe calibration (seesection 7.3.1)are function in factors entered the CALIBRATION out of the factorlimits. (seesection - When laboratory values 8.2)are LAB.LIMITS in entered the CHANGE limit the out of the limits. - When flagvalues (see 8.2.3) outof the are function section ADJUST in entered theFLAG the limits. (see - When automatic function CLEANING in value entered theAUTO frequency cleaning the limits. is 8.3.4) outof the section NOTE with are values availables displayed theco and Maximum minimum indications. to the the Correct values
error

is whenthe instrument turned :This message displayed is O - "STARTUP NOTINITIATED" carried (seesection out 8.3.3). ThisSTARTUP on, andthe startup cycleis notautomatically in to the from cycle order flush instrument thecleaning cycle mandatory each is STANDBY after is to reagent. blankcycleon reagents performed checkthe cleanliness the instrument. of A pressthe STARTUP is analysis requested, Whenthis message displayed a sample is and cyclekey. : is whenthe instruREAGENTS"Thismessage displayed P - "STARTUP FAILED, CHECK (seesection gives of range startup values 3 cycles 6.3).Check ment after consecutive out blank if or a cleaning the the expiration dates, replace reagents necessary perform concentrated 9.1.3.4. section according the procedure to described gives is whenthe instrument : out FAILED,,thismessage displayed Q - "HGB REFERENCE (seesection HGB blank cycles 6.3). HGBblank values 3 of ranges after consecutive reference if or the reagents necessary performa concentrated Checkthe expiration dates, replace section 9.1.3.4. described cleaning to according theprocedure :this LASTOPERATION> message displayed is when BETWEEN R - (4 HOURS ELAPSED In 4 for to any has used at least hours. order prevent driftintheresults, theinstrument notbeen is key a the to has before sample run.Press ENTER in order run a STARTUP to be performed the STARTUP. has Elseruna STANDBY cycleif the instrument to be stopped.

9.4. PURPOSE THE VALVES OF Valve #1 Valve #2 Valve#3 Valve#4 Valve#5 Valve #6 Yalve#7 Valve #8 the Controls lysedistribution in the syringe the Cancels pressure/vacuum pressure/vacuum and to of Allows bubbling thefirstdilution thetransfer the WBCchamber the headduring rinsing the inputin theWBCcounting the Controls cleaner syringe the Controls drainof the pressure/vacuum heads in needed the WBC& RBCcounting Activates vacuum the headduring rinsing the inputin the RBCcounting the Controls diluent input inside needle rinseblock the of the Controls aspiration thediluent/air
needle inside aspiration the Valve the #10 Controls diluent distribution Valve the #11 Controls diluent the Valve #12 Controls drainof the WBCchamber Valve the #13 Controls drainof the RBCchamber
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2 WAYS NC
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2 VOIESNF
3 WAYS
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Diag.9-10
6'6
3 VOIES

9.5.MENU OVERVIEW
ABC Vef
'- - Calibration Bloodcards- - -' Withoutpack
VETERINARY CARDS
RAM 030 A lnd.C

DIAGRAM 9.6.PNEUMATIC
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Scil ABC Vet User Manual

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ABC VetUserManual

Part number: RAB 015A Ind.E

nt" A" Caducee Parc buromddecine 34184MONTPELLIER Cedex 04 FRANCE

DECLARATION CON FORMITE DE DECLARATION CON FORMITY OF

ParcEurom6decine ruedu Caduc6e BP7290 MONTPELLIER 34184 4 Cedex FRANCE

NOUSLE FABRICANT WETHEMANUFACTURER

E N5 5 0 1 1 l a s sB E d .1 9 9 1 C . E N6 1 0 0 0 - 3 - 2 E d . 1 9 9 5 EN61000-3-3 1998 Ed. DECEMBRE 2OOO DECEMBER 2OOO

Emphasizes operating proceduresthat must be followed to avoid erroneousresults.

operational function. during aftera specific or

Section5 : Description Section6 : Startupand sample run

and troubleshooting Section9 : Maintenance

R A B0 1 5A 20520 12 20520 13 20520 14 205 20 32 D A C0 1 1A D A C0 1 2A

R A B0 1 5A 20520 63 20520 64 20520 65 20520 73 D A C0 1 1A D A C0 1 2A

(european) Power cable or (US) cable Table 2-01 Power

- TheABC Vet "pack" kit : installation includes DESIGNATION

PARTNUMBER GBC069A FAA036 A FAA053 A X E A3 1 2A

Oring6x1.5 O ring1,4 x 1,25 Table 2-03 kit installation Common

MAB OO2 A MAB OO3 A MAB 069 A

EAE OO7 A EAE OO9 A EAB032A

Diag.2-05 block 1 - Electrode 3 - RBCchamber 2 - Clip 4 - Mixing chamber

DILUANT DECHETS DILUENT I4IASIES

SWITCH 1 - ON/OFF HOLDER 2 - FUSE PLUG SUPPLY 3 - MAIN

4 - RS232 OUTPUT 5 - PRINTER OUTPUT

documents read Warning: enclosed

See printer documentationfor rnoredetails

Move cursor C the ro

PACK key. REAGENT andpress ENTER Select function the the O

- Option smart : card

Chien Dog " I Chat/ Cat * Cheval Horse I

Co0hon Pig / Mouton Sheep I

* Those with are smart cards delivered theinstrument

17,5m1 17,5m| 6,0m1

- Option: Reagent pack - Measuring principles :

5 to 11x 106/rnmo 8 to 20 g/dl

MCH MCHC RDW Lymphocytes(o/o) (%) Monocytes Granulocyte$f1o) Table 3-03

LNEARTTY RANGE 0,5to80 0.2to 7.5 10to 1000 2.5to 23

(whichevergreater) +/-5 or +l- SYo is

HGB 23.4 0.0

0.995 0.990 0.985 0.982 0.980 0.926

0.895 0.926 0.863 0.970

(%) Lymphocytes M o n o n u c l e a( % ) rs (%) Granulocytes

in is instrument not available "bottle"instrument TheABCVet16P(LMGE) type.

generated the wastechamber in vacuum Q places.

. liquiOvalves route fluids the required the to

blockQ . fn. dilution

Themixing chamber time. needle thesecond for sampling S Q

generated thewaste into chamber Ot mean thevacuum of Q

into cnamOer is drained thewaste @

't transferred the WBC to

in usingthe vacuumgenerated the wastechamber(S .nt the sametime,the

into lyse O.56mlof solution theWBCchambe, moves anddelivers up Q

cnamOer tou", overthemixing @

in chamber diluent themixing Q

is drained the waste into

is rou". intothe RBCchambet @ . Diluent sent

part into to theexternal of theneedle the RBCchamber.

SOLUTIOH AHALYSER A SOLUTIOH EE AHALYSED TO

Wecanderive fromOhm's : law U = Voltage

3 Cover part 4 Doorto pneumatic

door carriage SamPling panel Front

' vg;,3$"o ,l 3i"

sienr cAtiBAu lrt $ru nnn-r1t{ sc ro'ExrfCAI