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    Aquatic Microbiology

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    Renanda Baghaz Putra
    1) The document discusses cyanotoxins from blooming microcystis in a lake. Silver carp and bighead carp were introduced to control the blooms through biomanipulation. 2) Portulaca oleracea was tested for its ability to detoxify microcystins through absorption. The plant was able to decrease microcystin levels in culture medium. 3) Extracts from Microcystis aeruginosa were found to have antimycobacterial properties, inhibiting the growth of nontuberculous mycobacteria species at low concentrations.

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    Aquatic Microbiology

    Uploaded by

    Renanda Baghaz Putra
    116 views14 pages
    1) The document discusses cyanotoxins from blooming microcystis in a lake. Silver carp and bighead carp were introduced to control the blooms through biomanipulation. 2) Portulaca oleracea was tested for its ability to detoxify microcystins through absorption. The plant was able to decrease microcystin levels in culture medium. 3) Extracts from Microcystis aeruginosa were found to have antimycobacterial properties, inhibiting the growth of nontuberculous mycobacteria species at low concentrations.

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    Aquatic microbiology.ppt

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    1) The document discusses cyanotoxins from blooming microcystis in a lake. Silver carp and bighead carp were introduced to control the blooms through biomanipulation. 2) Portulaca oleracea was tested for its ability to detoxify microcystins through absorption. The plant was able to decrease microcystin levels in culture medium. 3) Extracts from Microcystis aeruginosa were found to have antimycobacterial properties, inhibiting the growth of nontuberculous mycobacteria species at low concentrations.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    116 views14 pages

    Aquatic Microbiology

    Uploaded by

    Renanda Baghaz Putra
    1) The document discusses cyanotoxins from blooming microcystis in a lake. Silver carp and bighead carp were introduced to control the blooms through biomanipulation. 2) Portulaca oleracea was tested for its ability to detoxify microcystins through absorption. The plant was able to decrease microcystin levels in culture medium. 3) Extracts from Microcystis aeruginosa were found to have antimycobacterial properties, inhibiting the growth of nontuberculous mycobacteria species at low concentrations.

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    of 14

    Cyanotoxin of Blooming Microcystin

    Oleh :
    RENANDA BAGHAZ D.S.P.
    Introduction

    Enrichment nutrien

    Blooming microcystis

    cyanotoxin
    Material and Method
    Biomanipulation

    Restocking
    3 Fish pen size
    Silver carp,
    0,36 km2
    Bighead carp

    Sampling per
    month , growth, plankton
    gut
    Material and Method
    Sampling

    30 silver carp and 30 bighead carp were randomly captured

    examine individual body length and body weight

    examine food items, 5 individuals of each carp and the guts removed
    in each month

    Intracellular microcystins were measured by a reverse-phase high-


    performance liquid chromatography (HPLC)
    Material and Method
    Detoxification with Portulaca oleracea

    P. oleracea cv. was grown under a germ-free condition on


    MurashigeeSkoog medium (3% sucrose and 0.2% gellan gum)
    in a culture bottle

    The propagation of the aseptic plants was carried out


    by stem cuttings on the same medium

    the plantlets were pre-cultured I with various concentrations of MC-


    LR, 0, 0.02, 0.2, or 2 mg/ml of MCLR, and after 7 days the amount of
    MC-LR in the medium and plant extracts were determined

    For the extraction of MC-LR from P. oleracea cv., freeze-dried


    plant samples were grinded with a mortar The sample was centrifuged
    at 140 0 g for 20 min, and the supernatant was collected and analyzed
    with HPLC
    Material and Method
    Antimycobacterial activity of microcystins

    m.aeruginosa Microcystin Evaluation of


    Isolates
    antimycobacterial
    extracts analysis mycobacterium spp
    activity

    •the cells were • Analysis of microcystin • M. aeruginosa extract, • the assay is


    obtained [D-Leu1] MC-LR, and accomplished on
    dissolved in from Microcystis RST microcystin-LR toxins microplates (96 wells)
    absolute methanol, 9501 was performed were tested against using resazurin as
    •sonicated three using the HPLC the nontuberculous indicator of cellular
    times and equipment Shimadzu mycobacteria: M. viability.
    SCL-10AVP (Japan). terrae (ATCC15755), M. • Medium 7H9 enriched
    centrifuged chelonae (ATCC 946) with 10 % OADC was
    (10,000 × g) at 4°C, and M. employed. The
    for ten minutes. kansasii(ATCC12478). extracts and pure
    Extracts were microcystin were
    evaporated at 40°C weighed, dissolved in
    DMSO
    • and the determination
    of minimal inhibitory
    concentration (MIC)
    Result
    Biomanipulation

    Blue points represent inflow water; Red points represent outflow water.

    Figure 4. Temporal changes of Microcystis biomass and MC concentration


    in both the fish pens and the surrounding lake from April to November.
    Result
    Biomanipulation
    Result
    Detoxification with Portulaca oleracea

    FIG. 1. Changes in MC-LR concentrations and protein phosphatases inhibition activities


    in the medium treated with P. oleracea cv. The initial MC-LR concentration was 0.02
    mg/ ml, and the medium was treated with P. oleracea cv. for 7 days. Open circle: MC-LR
    concentration in the medium treated with P. oleracea cv., open triangle: MC-LR
    concentration of control culture, closed circle: protein phosphatases inhibition activity
    in the medium treated with P. oleracea cv., and closed triangle: protein phosphatases
    inhibition activity of control culture.
    Result
    Antimycobacterial activity of microcystins
    Result
    Antimycobacterial activity of microcystins
    Result
    Antimycobacterial activity of microcystins
    Conclusion

    Biomanipulation using silver carp and Bighead


    carp significantly can remove MC

    Detoxification using Portulaca oleracea as


    detoxificator able to decrease microcystins
    through absorbtion when blooming in aquatic

    Microcystin can use as antimicrobial because it


    able to inhibition and inactivation activity of
    bacteria
    SEKIAN & TERIMA KASIH

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