Gen Lab Training FULL
Gen Lab Training FULL
Gen Lab Training FULL
September 2002
This training presentation has been created
for those who are required to undergo
general laboratory safety training and have
not taken this training with their supervisor.
It is also recommended for those
laboratories that do not fall under regulation,
but are needing a safety laboratory overview.
There are hyperlinks throughout the presentation. Where there is the following
icon additional training is required. Training
Introduction
Training
Know Your Surroundings
Training
Hazards in your Lab
Training
Hazards in your Lab
No open-toed shoes
No shorts unless a lab coat is used
Restrain hair when working with hazardous
materials
Remove protective clothing in public
Use the proper Personal Protective Equipment for
the job
Personal Habits
MSDS Content
Click here!
Reading the MSDS
MSDS
Hazard Communication
Training
Exposure Control Plan
The Exposure Control Plan is required for the labs that
have exposure to human blood or Other Potentially
Infectious Material (OPIM). This includes human
blood or human blood products such as semen or
vaginal secretions. This also includes fluids such as:
cerebrospinal, synovial, pleural, pericardial, peritoneal
and amniotic. Saliva and body fluid that is visibly
contaminated with blood also is considered OPIM.
Exposure Control Plan
Safety glasses
Gloves
Lab coat
Respirator (Depending on the agent and whether or not an
aerosol is created)
When to use PPE
www.animalpicturesarchive.com
Animals & PPE
When animals are being used the following
PPE must be used:
Gloves-May need a variety to prevent animal bites.
Lab coat-Needed to protect clothing and used as a barrier. To
Safety glasses-Must be
prevent the pathogen from leaving the lab, the lab coat must be
worn and must not leave the
lab.
worn to prevent possible injury to the
Training
Use of PPE
http://www.ehrs.upenn.edu/training/bloodborne/bloodborne.html
Safe Practices
is also impervious to moisture. The
sharps container must be labeled either
with “Biohazard” or “Infectious Waste”.
http://www.ehrs.upenn.edu/training/bloodborne/bloodborne.html
Disposal of Sharps
Training
What is a Biological Safety Level
(BSL)?
Biological Safety Level
Training
BSL-1 Laboratory
BIOHAZARD
All Personal Protective Equipment shall be removed prior to leaving this work area.
Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area
prohibited in this work area.
________________________________________________________________ __________________
Transmission route(s):
________________________________________________________________ __________________
________________________________________________________________ __________________
Emergency contacts
Name: ________________________________ Title: ______________ Phone: _______________
________________________________ _______________ _______________
________________________________ _______________ _______________
EH&S office (M-F 8-5) 646-3327 (after hours) 911.
________________________________________________________________ __________________
*Biosafety Level 2 is similar to BSL-1 and is suitable for work involving agents of moderate potentia l
hazard to personnel and the environment. It differs from BSL -1 in that
1.Lab personnel have specific training in handling pathogenic ag ents and directed to competent scientist;
2. Access to the lab is limited when work is being conducted:
3. Extreme precautions are taken with contaminated sharp items and
4. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological
safety cabinets or other physical containment equipment
Escherichia coli
Precautions:
Must use Eye protection, lab coat, gloves
Only use under BSC
Labeling
Chemical labeling has been briefly touched on earlier
in the presentation. One must remember that if any
chemical is transferred to a secondary container, this
container must be labeled. If the chemical will be
used by the end of the work shift, then labeling is
not necessary. Good science practices would
encourage you to label all containers.
Eating, drinking, smoking applying cosmetics or lip balm and handling contacts lenses area
prohibited in this work area.
__________________________________________________________________________________
Transmission route(s):
__________________________________________________________________________________
__________________________________________________________________________________
Emergency contacts
Name: ________________________________ Title: ______________ Phone: _______________
________________________________ _______________ _______________
take.
Containment of Laboratory
Containment
Laboratory Practices
Containment Equipment
Special laboratory design
Fume hood
Autoclave
Fume Hood
Gloves
Lab Coat
Shoe Covers
Safety Glasses
Respirator
Use of BSC
Toxic
Corrosive
Ignitable
Flammable
Oxidizer
Waste
http://www.lbl.gov/ehs/Medical/html/biohazardous.htm
Proper Disposal
EHS 438-8325
You have now completed the General Lab Safety
Training presentation. If you have any questions
please take the time to ask your supervisor or call
EHS. By signing the training form, you are
stating that you have completed the training and
that you understand the content within the
training. Please fill out the Certificate of
Completion on the next slide & turn into
your immediate supervisor.