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    Estimation of PCV by Wintrobe Method

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    jyoti singh
    This document describes the Wintrobe method for estimating packed cell volume (PCV). PCV, also known as hematocrit, measures the ratio of red blood cell volume to total blood volume as a percentage. The Wintrobe method involves filling a specialized tube containing anticoagulated whole blood all the way to the 100 mark and centrifuging. The length of the packed red blood cell column is then read directly from the tube to determine PCV. Normal PCV ranges are provided for males, females, pregnant females, and newborns. PCV is useful for diagnosing anemia and polycythemia and supporting dengue fever diagnosis.

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    Estimation of PCV by Wintrobe Method

    Uploaded by

    jyoti singh
    455 views18 pages
    This document describes the Wintrobe method for estimating packed cell volume (PCV). PCV, also known as hematocrit, measures the ratio of red blood cell volume to total blood volume as a percentage. The Wintrobe method involves filling a specialized tube containing anticoagulated whole blood all the way to the 100 mark and centrifuging. The length of the packed red blood cell column is then read directly from the tube to determine PCV. Normal PCV ranges are provided for males, females, pregnant females, and newborns. PCV is useful for diagnosing anemia and polycythemia and supporting dengue fever diagnosis.

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    ESTIMATION OF PCV BY WINTROBE METHOD

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    This document describes the Wintrobe method for estimating packed cell volume (PCV). PCV, also known as hematocrit, measures the ratio of red blood cell volume to total blood volume as a percentage. The Wintrobe method involves filling a specialized tube containing anticoagulated whole blood all the way to the 100 mark and centrifuging. The length of the packed red blood cell column is then read directly from the tube to determine PCV. Normal PCV ranges are provided for males, females, pregnant females, and newborns. PCV is useful for diagnosing anemia and polycythemia and supporting dengue fever diagnosis.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    455 views18 pages

    Estimation of PCV by Wintrobe Method

    Uploaded by

    jyoti singh
    This document describes the Wintrobe method for estimating packed cell volume (PCV). PCV, also known as hematocrit, measures the ratio of red blood cell volume to total blood volume as a percentage. The Wintrobe method involves filling a specialized tube containing anticoagulated whole blood all the way to the 100 mark and centrifuging. The length of the packed red blood cell column is then read directly from the tube to determine PCV. Normal PCV ranges are provided for males, females, pregnant females, and newborns. PCV is useful for diagnosing anemia and polycythemia and supporting dengue fever diagnosis.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 18

    ESTIMATION OF PCV

    BY WINTROBE
    METHOD
    PACKED CELL VOLUME
    • DEFINITION : PCV is defined as the
    ratio of volume of RBC’s to that of
    whole blood and is expressed as
    percentage.
    • Also known as hematocrit and
    erythrocyte volume fraction.
    HAEMATOCRIT
    • Earlier PCV and haematocrit were considered synonymous but
    now International Committee for Standardazition in
    Hematology, has said the term
    • PCV= traditional technique by centrifugation
    • Haematocrit= derivation on automated hematology analyzers.
    For understanding don’t copy in manual
    • Blood =plasma + cells.
    • This cells quantity in percentage is PCV .
    • Cells mostly are RBCs, so we here would be referring to RBCs
    only.
    REFERENCE RANGES FOR PCV
    • Adult Male : 40-50%
    • Adult Female : nonpregnant : 38-45%
    • Adult Female : pregnant : 36-42%
    • Newborn : 44-60%
    Uses of PCV
    • Diagnosis of anemia or polycythemia
    • Estimation of red cell indices(MCV & MCHC)
    • Checking accuracy of hemoglobin value
    • Smears can be made from buffy coat layer for detection of LE
    cells, malaria parasites, microfilaria, trypanosome, or blast
    cells
    • Supportive evidence for dengue haemorrhagic fever
    Methods for Estimation of
    PCV
    • Wintrobe’s method (Macro method)
    • Micro haematocrit method
    • Electronic method
    WINTROBE METHOD
    • PRINCIPLE : Anti-coagulated whole blood is
    centrifuged in a Wintrobe tube to
    completely pack the red cells.
    • The volume of packed red cells is read
    directly from tube.
    EQUIPMENTS
    1. WINTROBE TUBE: 110mm in length and 100 markings, each
    at the interval of 1mm, interval diameter 3mm. It can hold
    about 1mL of blood.
    2. PASTEUR PIPETTE
    3. CENTRIFUGATION MACHINE
    SPECIMEN
    • Venous blood collected in ethylene-di-
    amine-tetra-acetic acid (EDTA) vial (1.5 mg
    EDTA for 1 mL of blood).
    • Performed within 6 hours of collection
    METHODS
    • Mix the anti-coagulated blood sample throughly
    • Draw the blood sample in a Pasteur pipette and
    introduce the pipette up to the bottom of the
    Wintrobe tube. Fill the tube from the bottom
    exactly up to the 100 mark(1mL of blood).
    • Centrifuge the sample at 2,300 rpm for
    30minutes.
    • Take the reading of the length of the column of
    red cells.
    • After centrifugation layers are noted as :
    1. Upper most layer of plasma
    2. Platelets (thin white layer )
    3. Greyish pink layer of leucocyte
    4. Lower most is layer of RBC
    Advantages of Macro-method
    1. PCV and ESR is estimated simultaneously
    2. Buffy coat can be prepared for LE cells.
    3. By seeing the colour of the plasma, some
    pathological can be known.example :
    a) Yellow colour – Jaundice
    b) Pink colour – haemolysis
    c) Milky colour -- hyperlipidaemia
    Causes of raised PCV
    • Polycythemia
    • Burns
    • shock
    Causes of low PCV
    • Anaemia
    • pregnancy
    Formula
    • PCV = 3 X HAEMOGLOBIN.
    (GRAM PER DECILITRE)
    • PCV = 9 X RBC COUNT(MILLION
    PER CUBIC MM)

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