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    Biotin

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    Scindiaa
    Biotin is a B vitamin that acts as a coenzyme in carboxylation reactions. It has an imidazole ring structure and binds to carboxylase enzymes through its valeric acid chain. Biotin is found in many foods and intestinal bacteria can synthesize it. As a coenzyme, biotin facilitates the transfer of carbon dioxide to substrates through carboxylation reactions involved in fatty acid synthesis and the citric acid cycle. Biotin deficiency can result from long-term antibiotic use and causes symptoms like dermatitis. Adults require 30-40 micrograms of biotin per day.

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    Biotin

    Uploaded by

    Scindiaa
    19 views21 pages
    Biotin is a B vitamin that acts as a coenzyme in carboxylation reactions. It has an imidazole ring structure and binds to carboxylase enzymes through its valeric acid chain. Biotin is found in many foods and intestinal bacteria can synthesize it. As a coenzyme, biotin facilitates the transfer of carbon dioxide to substrates through carboxylation reactions involved in fatty acid synthesis and the citric acid cycle. Biotin deficiency can result from long-term antibiotic use and causes symptoms like dermatitis. Adults require 30-40 micrograms of biotin per day.

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    Biotin is a B vitamin that acts as a coenzyme in carboxylation reactions. It has an imidazole ring structure and binds to carboxylase enzymes through its valeric acid chain. Biotin is found in many foods and intestinal bacteria can synthesize it. As a coenzyme, biotin facilitates the transfer of carbon dioxide to substrates through carboxylation reactions involved in fatty acid synthesis and the citric acid cycle. Biotin deficiency can result from long-term antibiotic use and causes symptoms like dermatitis. Adults require 30-40 micrograms of biotin per day.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    19 views21 pages

    Biotin

    Uploaded by

    Scindiaa
    Biotin is a B vitamin that acts as a coenzyme in carboxylation reactions. It has an imidazole ring structure and binds to carboxylase enzymes through its valeric acid chain. Biotin is found in many foods and intestinal bacteria can synthesize it. As a coenzyme, biotin facilitates the transfer of carbon dioxide to substrates through carboxylation reactions involved in fatty acid synthesis and the citric acid cycle. Biotin deficiency can result from long-term antibiotic use and causes symptoms like dermatitis. Adults require 30-40 micrograms of biotin per day.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 21

    BIOTIN

    By: Jeyanth S.
    OBJECTIVES
     Structure
     Sources
     Metabolism
     Active form
     Coenzyme activity
     Antagonists
     Deficiency
     Requirement
    STRUCTURE
    Imidazole
    ring

    Valeric
    acid
    Thiophene
    ring
    INTRODUCTION
     Known as anti-egg white injury factor.
     Formerly known as vitamin H and vitamin
    B7
     found that rats fed large amounts of egg-
    white as their only protein source exhibited
    neurological dysfunction, hair loss,
    dermatitis, and eventually, death.
    SOURCES
    METABOLISM
     Biotin is found as biocytin in many foods, readily
    absorbed.
     Taken up by liver and muscle.
     Bacterial flora will provide adequate quantities
    of biotin
     Because it is also synthesised by intestinal flora,
    excretion is 3-6 times greater than intake.
    ACTIVE FORM
     BIOCYTIN
     Valeric acid chain binds with epsilon amino group
    of lysine in enzyme
    CO-ENZYME ACTIVITY
     Essential for carboxylation reactions
     ABC of carboxylation reactions
     A – ATP
     B - Biotin
     C – CO2 and Carboxylase enzyme
    ACETYL COA CARBOXYLASE
    PYRUVATE CARBOXYLASE

    • It provides OAA for TCA cycle and


    Important in the glucogenic pathway.
    PROPIONYL COA CARBOXYLASE
    BIOTIN INDEPENDENT CARBOXYLATION
    REACTIONS
     Carbamoyl phosphate synthesis in Urea Cycle.
    CPS 1 AND CPS 2
     C6 in Purine ring.
     Malic enzyme converting pyruvate to malate.
    BIOTIN ANTAGONISTS
     Avidin
     A protein present in egg white- has a great affinity to biotin
     Intake of raw boiled egg causes biotin deficiency
     One molecule of avidin binds with 4 molecules of biotin
     Boiling of egg will neutralize its inhibitory activity
     Streptavidin
    USES
    ELISA TEST
    Biotin
    Labelling of
    DNA
    DEFICIENCY
     During prolonged use of antibacterial drugs
     Symptoms- dermatitis
    atrophic glossitis
    hyperesthesia
    muscle pain
    anorexia
    split nails
    REQUIREMENTS
     30-40µg for adults
     100-300µg in treating deficiency
    QUESTION
     Biotin acts as a coenzyme for all except:
    a. Pyruvate to Oxaloacetate
    b. Acetyl CoA to Malonyl CoA
    c. Propionyl CoA to Methyl Malonyl CoA
    d. Glutamate to Gamma Carboxy glutamate

    Answer: D

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