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Anupama Awadhesh Pandey

BIOTECH PPT

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Anupama Awadhesh Pandey

BIOTECH PPT

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s1032190456
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ANUPAMA AWADHESH PANDEY

TITLE
INVESTIGATING NOVEL PLASTIC-DEGRADING BACTERIA FROM
DUMPED SOIL AREAS FOR SUSTAINABLE PLASTIC WASTE
MANAGEMENT
INTRODUCTION
 Plastic has become an indispensable part of our lives and cutting down plastic consumption
entirely is difficult to achieve.

 The recalcitrant and non-biodegradable nature of plastic leads to the accumulation of tons of
plastic in landfills and water bodies, which further risks marine life and human life, causing serious
health issues.

 Plastic pollution is a global environmental crisis, with millions of tons of plastic waste
generated annually.

 Traditional methods of plastic disposal are inadequate, leading to an urgent need for innovative
solutions.
INTRODUCTION
 Recent studies have identified specific bacteria capable of degrading plastics, presenting a
promising avenue for bioremediation.

 Understanding plastic degradation's genetic and metabolic pathways is crucial for


bioremediation strategies.

 This research aims to explore novel plastic-degrading bacteria, characterize their enzymatic
mechanisms, and assess their potential for practical applications in waste management.
REVIEW OF LITERATURE
 The literature on plastic-degrading bacteria in waste management highlights the urgent need for
advancements in plastic waste management due to the significant environmental concerns
posed by plastic litter accumulation (Chigwada & Tekere, 2023).

 Additionally, microbial consortia or cocultures are recognized as cost-effective and eco-


friendly alternatives to conventional plastic waste management methods, potentially converting
by-products of microbial degradation into bio-energy sources (Skariyachan et al., 2021).

 Contradictorily, while bacterial degradation is deemed the most effective biodegradation


approach, our understanding of the mechanisms and strategies to enhance this process remains
limited (He et al., 2023).
REVIEW OF LITERATURE
 Moreover, despite the identification of microorganisms capable of degrading plastic waste, the
implementation of these biological treatment strategies on a commercial scale is still in its infancy
(Fernández et al., 2022; Shah, 2020).

 However, further research is necessary to optimize these biological processes and translate them
into viable commercial applications (Fernández et al., 2022; He et al., 2023).

 The future of plastic waste management is likely to be shaped by advancements in our


understanding of microbial degradation mechanisms and the strategic enhancement of
biodegradation capabilities (He et al., 2023; Yang et al., 2023).

 An excellent but perhaps overlooked tool for the study of microbial activity in the soil, nutrient
cycling, microbial succession, and ecology is the Winogradsky column (Pigage et al., 1985).
AIMS AND OBJECTIVES
 Isolation and Characterization: Isolate novel plastic-degrading bacteria from contaminated
environments thriving in both aerobic and anaerobic conditions.

 Enzymatic Analysis: Characterize the enzymes involved in plastic degradation.

 Metabolic Pathway Elucidation: Investigate metabolic pathways associated with plastic


degradation.

 Bioremediation Potential: Evaluate the effectiveness of isolated bacteria in real-world plastic


waste scenarios.
METHODOLOGY
1. Sample Collection
• Locations: Collect soil and water samples from plastic-polluted sites, such as landfills, rivers, and
coastal areas.

2. Isolation of Bacteria
•Serial Dilution
•Spread Plate Technique

3. Screening for Degradation Activity


• Agar cup Method
• Clear zone Method
• Streak Method
METHODOLOGY
4. Winogradsky Column
• Evaluate bacterial growth on plastic substrates and measure degradation rates using weight loss
and visual inspection over time under anaerobic conditions.

5. Identification
• Morphological: i) Gram Staining
ii) Colony Characteristics
• Biochemical Test
METHODOLOGY
6. Enzymatic Characterization

• Enzyme Assays: Identify and characterize enzymes involved in plastic degradation through
biochemical assays.
• Molecular Techniques: Perform gene sequencing and analysis to identify key plastic-
degrading enzymes.

7. Metabolic Pathway Analysis

• Bioinformatics Tools: Utilize genomic and transcriptomic data to reconstruct metabolic


pathways involved in plastic degradation using databases like KEGG and MetaCyc.
 Expected Outcomes

•Identification of novel plastic-degrading bacteria with high degradation efficiency.


•Characterization of key enzymes and metabolic pathways involved in plastic breakdown.
• Practical recommendations for using these bacteria in bioremediation strategies for plastic waste
management.

 Significance of the Study

• This research will contribute significantly to the field of environmental microbiology and
bioremediation by providing insights into effective biological solutions for plastic pollution. The
findings could lead to the development of sustainable practices for managing plastic waste and
restoring contaminated environments.
CONCLUSION
• This research proposal outlines a comprehensive approach to investigating plastic-degrading
bacteria, aiming to contribute valuable insights and practical solutions to the plastic pollution
crisis. Through the isolation, characterization, and application of these microorganisms, we hope
to advance the field of environmental biotechnology and promote sustainable waste management
practices.

• Recent advancements in the study of plastic-degrading bacteria demonstrate significant progress


in identifying new species, characterizing their enzymes, and applying this knowledge to practical
solutions for plastic pollution. Continued research in this field is crucial for developing
sustainable strategies to combat plastic waste effectively.
REFERENCES
 Yoshida, S., et al. (2016). "A bacterium that degrades and assimilates poly(ethylene terephthalate).
Science.“
 Ryu, H. W., et al. (2018). "Biodegradation of plastics by Pseudomonas.“ Environmental
Microbiology Reports.
 Kawai, F., et al. (2014). "Isolation of bacteria capable of degrading polyurethane.“ Applied
Microbiology and Biotechnology
 Hadad, D., Geresh, S., & Sivan, A. (2005). Biodegradation of polyethylene by the thermophilic
bacterium Brevibacillusborstelensis. Journal of Applied Microbiology, 98(5), 1093-1100. 4.
 Russell JR, Huang J, Anand P, Kucera K, Sandoval AG, Dantzler KW, Hickman D, Jee J, Kimovec
FM, Koppstein D, Marks DH,( 2011), Biodegradation of polyester polyurethane by endophytic
fungi. Appl Environ Microbiol., 77(17): 6076-6084
THANK YOU

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