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===Structure===
===Structure===
[[File:HBV.png|thumb|The structure of hepatitis B virus]]
[[File:HBV.png|thumb|The structure of hepatitis B virus]]
Hepatitis B virus is a member of the [[Hepadnaviridae|Hepadnavirus family]].<ref name=Baron>{{cite book | author = Zuckerman AJ | title = Hepatitis Viruses. ''In:'' Baron's Medical Microbiology ''(Baron S et al., eds.)| edition = 4th | publisher = Univ of Texas Medical Branch | year = 1996 | url = http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.section.3738 | isbn = 0-9631172-1-1 }}</ref> The virus particle, ([[virion]]) consists of an outer [[lipid]] envelope and an [[icosahedron|icosahedral]] [[nucleocapsid]] core composed of [[protein]]. The nucleocapsid encloses the viral DNA and a DNA polymerase that has [[reverse transcriptase]] activity similar to retroviruses.<ref name="pmid15192795">{{cite journal |author=Locarnini S |title=Molecular virology of hepatitis B virus |journal=Semin. Liver Dis. |volume=24 |issue=Suppl 1 |pages=3–10 |year=2004 |pmid=15192795 |doi=10.1055/s-2004-828672}}</ref> The outer envelope contains embedded proteins which are involved in viral binding of, and entry into, susceptible cells. The virus is one of the smallest enveloped animal viruses with a virion diameter of 42&nbsp;nm, but [[pleomorphism (microbiology)|pleomorphic]] forms exist, including filamentous and spherical bodies lacking a core. These particles are not infectious and are composed of the lipid and protein that forms part of the surface of the virion, which is called the surface antigen ([[HBsAg]]), and is produced in excess during the life cycle of the virus.<ref name="pmid3014045">{{cite journal |author=Howard CR |title=The biology of hepadnaviruses |journal=J. Gen. Virol. |volume=67 |issue= 7|pages=1215–35 |year=1986 |pmid=3014045 |doi=10.1099/0022-1317-67-7-1215}}</ref>
Hepatitis B virus is a member of the [[Hepadnaviridae|Hepadnavirus family]].<ref name=Baron>{{cite book | author = Zuckerman AJ | title = Hepatitis Viruses. ''In:'' Baron's Medical Microbiology ''(Baron S et al., eds.)| edition = 4th | publisher = Univ of Texas Medical Branch | year = 1996 | url = http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.section.3738 | isbn = 0-9631172-1-1 }}</ref> The virus particle, called Dane particle<ref>{{Cite web|title = WHO {{!}} Hepatitis B|url = http://www.who.int/csr/disease/hepatitis/whocdscsrlyo20022/en/index2.html|website = www.who.int|accessdate = 2015-07-12}}</ref> ([[virion]]), consists of an outer [[lipid]] envelope and an [[icosahedron|icosahedral]] [[nucleocapsid]] core composed of [[protein]]. The nucleocapsid encloses the viral DNA and a DNA polymerase that has [[reverse transcriptase]] activity similar to retroviruses.<ref name="pmid15192795">{{cite journal |author=Locarnini S |title=Molecular virology of hepatitis B virus |journal=Semin. Liver Dis. |volume=24 |issue=Suppl 1 |pages=3–10 |year=2004 |pmid=15192795 |doi=10.1055/s-2004-828672}}</ref> The outer envelope contains embedded proteins which are involved in viral binding of, and entry into, susceptible cells. The virus is one of the smallest enveloped animal viruses with a virion diameter of 42&nbsp;nm, but [[pleomorphism (microbiology)|pleomorphic]] forms exist, including filamentous and spherical bodies lacking a core. These particles are not infectious and are composed of the lipid and protein that forms part of the surface of the virion, which is called the surface antigen ([[HBsAg]]), and is produced in excess during the life cycle of the virus.<ref name="pmid3014045">{{cite journal |author=Howard CR |title=The biology of hepadnaviruses |journal=J. Gen. Virol. |volume=67 |issue= 7|pages=1215–35 |year=1986 |pmid=3014045 |doi=10.1099/0022-1317-67-7-1215}}</ref>


===Components===
===Components===

Revision as of 11:29, 12 July 2015

For the disease, see Hepatitis B.

Hepatitis B virus
TEM micrograph showing hepatitis B viruses
Virus classification
Group:
Group VII (dsDNA-RT)
Order:
Unassigned
Family:
Hepadnaviridae
Genus:
Orthohepadnavirus
Species:
Hepatitis B virus

Hepatitis B virus, abbreviated HBV, is a species of the genus Orthohepadnavirus, which is likewise a part of the Hepadnaviridae family of viruses.[1] This virus causes the disease hepatitis B.[2]

Disease

Main article: Hepatitis B

In addition to causing hepatitis, infection with HBV can lead to cirrhosis and hepatocellular carcinoma.[3]

It has also been suggested that it may increase the risk of pancreatic cancer.[2]

Classification

The hepatitis B virus is classified as the type species of the Orthohepadnavirus, which contains three other species: the Ground squirrel hepatitis virus, Woodchuck hepatitis virus, and the Woolly monkey hepatitis B virus. The genus is classified as part of the Hepadnaviridae family, which contains two other genera, the Avihepadnavirus and a second which has yet to be assigned. This family of viruses have not been assigned to a viral order.[4] Viruses similar to hepatitis B have been found in all apes (orangutan, gibbons, gorillas and chimpanzees), in Old World monkeys,[5] and in a New World woolly monkeys suggesting an ancient origin for this virus in primates.

The virus is divided into four major serotypes (adr, adw, ayr, ayw) based on antigenic epitopes present on its envelope proteins, and into eight genotypes (A–H) according to overall nucleotide sequence variation of the genome. The genotypes have a distinct geographical distribution and are used in tracing the evolution and transmission of the virus. Differences between genotypes affect the disease severity, course and likelihood of complications, and response to treatment and possibly vaccination.[6][7]

Unclassified species

A number of as yet unclassified Hepatitis B like species have been isolated from bats.[8]

Morphology

Structure

The structure of hepatitis B virus

Hepatitis B virus is a member of the Hepadnavirus family.[9] The virus particle, called Dane particle[10] (virion), consists of an outer lipid envelope and an icosahedral nucleocapsid core composed of protein. The nucleocapsid encloses the viral DNA and a DNA polymerase that has reverse transcriptase activity similar to retroviruses.[11] The outer envelope contains embedded proteins which are involved in viral binding of, and entry into, susceptible cells. The virus is one of the smallest enveloped animal viruses with a virion diameter of 42 nm, but pleomorphic forms exist, including filamentous and spherical bodies lacking a core. These particles are not infectious and are composed of the lipid and protein that forms part of the surface of the virion, which is called the surface antigen (HBsAg), and is produced in excess during the life cycle of the virus.[12]

Components

It consists of:

Hepatitis D virus requires HBV envelope particles to become virulent.[15]

Evolution

The early evolution of the Hepatitis B, like that of all viruses, is difficult to establish.

The divergence of orthohepadnavirus and avihepadnavirus occurred ~125,000 years ago (95% interval 78,297–313,500).[16] Both the Avihepadnavirus and Orthohepadna viruses began to diversify about 25,000 years ago.[16] The branching at this time lead to the emergence of the Orthohepadna genotypes A–H. Human strains have a most recent common ancestor dating back to 7,000 (95% interval: 5,287–9,270) to 10,000 (95% interval: 6,305–16,681) years ago.

The Avihepadnavirus lack a X protein but a vestigial X reading frame is present in the genome of duck hepadnavirus.[17] The X protein may have evolved from a DNA glycosylase.

The rate of nonsynonymous mutations in this virus has been estimated to be about 2×10−5 amino acid replacements per site per year.[18] The mean number of nucleotide substitutions/site/year is ~7.9×10−5.

A second estimate of the origin of this virus suggests a most recent common ancestor of the human strains evolved ~1500 years ago.[19] The most recent common ancestor of the avian strains was placed at 6000 years ago. The mutation rate was estimated to be ~10−6 substitutions/site/year.

Genome

The genome organisation of HBV. The genes overlap.

Size

The genome of HBV is made of circular DNA, but it is unusual because the DNA is not fully double-stranded. One end of the full length strand is linked to the viral DNA polymerase. The genome is 3020–3320 nucleotides long (for the full length strand) and 1700–2800 nucleotides long (for the short length strand).[20]

Encoding

The negative-sense, (non-coding), is complementary to the viral mRNA. The viral DNA is found in the nucleus soon after infection of the cell. The partially double-stranded DNA is rendered fully double-stranded by completion of the (+) sense strand by viral polymerase and removal of a protein molecule from the (-) sense strand and a short sequence of RNA from the (+) sense strand. Non-coding bases are removed from the ends of the (-)sense strand and the ends are rejoined.

There are four known genes encoded by the genome called C, P, S, and X. The core protein is coded for by gene C (HBcAg), and its start codon is preceded by an upstream in-frame AUG start codon from which the pre-core protein is produced. HBeAg is produced by proteolytic processing of the pre-core protein. The DNA polymerase is encoded by gene P. Gene S is the gene that codes for the surface antigen (HBsAg). The HBsAg gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced.[21] The function of the protein coded for by gene X is not fully understood,[22] but some evidence suggests that it may function as a transcriptional transactivator.

Several non-coding RNA elements have been identified in the HBV genome. These include: HBV PREalpha, HBV PREbeta and HBV RNA encapsidation signal epsilon.[23][24]

Genotypes

Genotypes differ by at least 8% of the sequence and have distinct geographical distributions and this has been associated with anthropological history. Within genotypes subtypes have been described: these differ by 4–8% of the genome.

There are eight known genotypes labeled A through H.[6]

A possible new "I" genotype has been described,[25] but acceptance of this notation is not universal.[26]

Two further genotypes have since been recognised.[27] The current (2014) listing now runs A though to J. Several subtypes are also recognised.

There are at least 24 subtypes.

Different genotypes may respond to treatment in different ways.[28][29]

Individual genotypes

Type F which diverges from the other genomes by 14% is the most divergent type known. Type A is prevalent in Europe, Africa and South-east Asia, including the Philippines. Type B and C are predominant in Asia; type D is common in the Mediterranean area, the Middle East and India; type E is localized in sub-Saharan Africa; type F (or H) is restricted to Central and South America. Type G has been found in France and Germany. Genotypes A, D and F are predominant in Brazil and all genotypes occur in the United States with frequencies dependent on ethnicity.

The E and F strains appear to have originated in aboriginal populations of Africa and the New World, respectively.

Type A has two subtypes: Aa (A1) in Africa/Asia and the Philippines and Ae (A2) in Europe/United States.

Type B has two distinct geographical distributions: Bj/B1 ('j'—Japan) and Ba/B2 ('a'—Asia). Type Ba has been further subdivided into four clades (B2–B4).

Type C has two geographically subtypes: Cs (C1) in South-east Asia and Ce (C2) in East Asia. The C subtypes have been divided into five clades (C1–C5). A sixth clade (C6) has been described in the Philippines but only in one isolate to date.[30] Type C1 is associated with Vietnam, Myanmar and Thailand; type C2 with Japan, Korea and China; type C3 with New Caledonia and Polynesia; C4 with Australia; and C5 with the Philippines. A further subtype has been described in Papua, Indonesia.[31]

Type D has been divided into 7 subtypes (D1–D7).

Type F has been subdivided into 4 subtypes (F1–F4). F1 has been further divided into 1a and 1b. In Venezuela subtypes F1, F2, and F3 are found in East and West Amerindians. Among South Amerindians only F3 was found. Subtypes Ia, III, and IV exhibit a restricted geographic distribution (Central America, the North and the South of South America respectively) while clades Ib and II are found in all the Americas except in the Northern South America and North America respectively.

Life cycle

Hepatitis B virus replication

The life cycle of hepatitis B virus is complex. Hepatitis B is one of a few known non-retroviral viruses which use reverse transcription as a part of its replication process.

Attachment
The virus gains entry into the cell by binding to a receptor on the surface of the cell and enters it by clathrin-dependent endocytosis. The cell surface receptor has been identified as the Sodium/Bile acid cotransporting pepetide SLC10A1 (also named NTCP).
Penetration
The virus membrane then fuses with the host cell's membrane releasing the DNA and core proteins into the cytoplasm.
Uncoating
Because the virus multiplies via RNA made by a host enzyme, the viral genomic DNA has to be transferred to the cell nucleus. It is thought the capsid is transported on the microtubules to the nuclear pore. The core proteins dissociate from the partially double stranded viral DNA is then made fully double stranded and transformed into covalently closed circular DNA (cccDNA) that serves as a template for transcription of four viral mRNAs.
Replication
The largest mRNA, (which is longer than the viral genome), is used to make the new copies of the genome and to make the capsid core protein and the viral DNA polymerase.
Assembly
These four viral transcripts undergo additional processing and go on to form progeny virions which are released from the cell or returned to the nucleus and re-cycled to produce even more copies.[21][32]
Release
The long mRNA is then transported back to the cytoplasm where the virion P protein synthesizes DNA via its reverse transcriptase activity.

See also

References

  1. ^ Hunt, Richard (2007-11-21). "Hepatitis viruses". University of Southern California, Department of Pathology and Microbiology. Retrieved 2008-03-13.
  2. ^ a b Hassan MM, Li D, El-Deeb AS; et al. (October 2008). "Association between hepatitis B virus and pancreatic cancer". J. Clin. Oncol. 26 (28): 4557–62. doi:10.1200/JCO.2008.17.3526. PMC 2562875. PMID 18824707. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
  3. ^ Schwalbe M, Ohlenschläger O, Marchanka A; et al. (March 2008). "Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element". Nucleic Acids Res. 36 (5): 1681–9. doi:10.1093/nar/gkn006. PMC 2275152. PMID 18263618. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
  4. ^ Mason, W.S.; et al. (2008-07-08). "00.030. Hepadnaviridae". ICTVdB Index of Viruses. International Committee on Taxonomy of Viruses. Retrieved 2009-03-13. {{cite web}}: Explicit use of et al. in: |author= (help)
  5. ^ Dupinay T; et al. (November 2013). "Discovery of naturally occurring transmissible chronic hepatitis B virus infection among Macaca fascicularis from Mauritius Island". Hepatology,. Vol. 58, no. 5. pp. 1610–1620. doi:10.1002/hep.26428. PMID 23536484. {{cite news}}: Explicit use of et al. in: |author= (help)CS1 maint: extra punctuation (link)
  6. ^ a b Kramvis A, Kew M, François G (2005). "Hepatitis B virus genotypes". Vaccine. 23 (19): 2409–23. doi:10.1016/j.vaccine.2004.10.045. PMID 15752827.{{cite journal}}: CS1 maint: multiple names: authors list (link) Cite error: The named reference "pmid15752827" was defined multiple times with different content (see the help page).
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  12. ^ Howard CR (1986). "The biology of hepadnaviruses". J. Gen. Virol. 67 (7): 1215–35. doi:10.1099/0022-1317-67-7-1215. PMID 3014045.
  13. ^ Guo GH, Tan DM, Zhu PA, Liu F (February 2009). "Hepatitis B virus X protein promotes proliferation and upregulates TGF-beta1 and CTGF in human hepatic stellate cell line, LX-2". Hbpd Int. 8 (1): 59–64. PMID 19208517.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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  26. ^ Kurbanov F, Tanaka Y, Kramvis A, Simmonds P, Mizokami M (August 2008). "When should "I" consider a new hepatitis B virus genotype?". J. Virol. 82 (16): 8241–2. doi:10.1128/JVI.00793-08. PMC 2519592. PMID 18663008.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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  30. ^ Cavinta L, Sun J, May A; et al. (June 2009). "A new isolate of hepatitis B virus from the Philippines possibly representing a new subgenotype C6". J. Med. Virol. 81 (6): 983–7. doi:10.1002/jmv.21475. PMID 19382274. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
  31. ^ Lusida M.I., Nugrahaputra V.E., Soetjipto Handajani R., Nagano-Fujii M., Sasayama M., Utsumi T., Hotta H. (2008). "Novel subgenotypes of hepatitis B virus genotypes C and D in Papua, Indonesia". J. Clin. Microbiol. 46 (7): 2160–2166. doi:10.1128/JCM.01681-07. PMC 2446895. PMID 18463220.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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