Sodium dodecyl-sulphate

Two-dimensional gel electrophoresis (2DE) is a two-dimensional technique for protein separation, which combines isoelectric focusing and sodium dodecyl sulphate (SDS) electrophoresis. The high resolving power results from separation according to charge (isoelectric point) in the first dimension and size (mobility in a porous gel) in the second dimension. Depending on the gel size, from several hundred to more than 5,000 proteins can be separated. [Pg.1252]

Escribano, J., Cabanes, J., and Garcfa-Carmona, R, Characterisation of latent polyphenol oxidase in table beet effect of sodium dodecyl sulphate, J. Sci. Food Agric., 73, 34, 1997. [Pg.97]

Site-specific desorption probability SDS Sodium dodecyl sulphate... [Pg.708]

Homogeneous LaMn03 nanopowder with the size of 19-55 nm and with the specific surface area of 17-22 m2/g has been synthesized using a surfactant, sodium dodecyl sulphate (SDS) to prevent agglomeration [47], The sonochemically prepared LaMn03 showed a lower phase transformation temperature of 700°C, as compared to the LaMn03 prepared by other conventional methods which has been attributed to the homogenization caused by sonication. Also, a sintered density of 97% of the powders was achieved for the sonochemically prepared powders at low temperature than that of conventionally prepared powders. [Pg.202]

Fig. 15.5 Long-exposure photographs recorded for argon-saturated water (a) and luminol solution (b) and (c) 10 mM sodium dodecyl sulphate (SDS) solution. The emission spectra in (d) are from pure argon-saturated water and 10 mM SDS solution. Note the sodium D line at 589 nm. In this experiment, sonication was performed at 159 kHz...

Modified CNTs feature various spectral changes depending on the methods and the location of modifications. These changes include variations in band frequencies, width, and intensities. For example, aryldiazonium salts [139] were used to modify individual sodium dodecyl sulphate (SDS) coated SWNTs with aryl group. The Raman spectrum of functionalized (SDS-free) SWNTs shows a disorder mode much higher than pristine SWNT the radial breathing modes are nearly unobservable. [Pg.507]

VCL N-vinylcaprolactam, SDS sodium dodecyl sulphate, MACUE042 polyethylene oxide)42 undecyl a-methacrylate, KPS potassium persulphate, VA-086 2,2 -azobis [ 2-methyl-N-(2-hydroxye thyl) propionamide ]... [Pg.55]

Sodium dodecyl sulphate polyacrylamide gel electrophoresis Single step isolation and identification procedure White rot fungi... [Pg.158]

Molecular masses of the same enzymes of different species are different. Molecular mass of the laccase of Pleorotus ostreatus was found to be 66.8 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) [48]. Purified enzyme of T. versicolor having a single band with a molecular mass of 68 kDa was in the same range with the molecular weights of laccase isoforms isolated from 2,5-xylidine-induced cultures of T. versicolor [49]. [Pg.163]

HTAC cationic micelles also markedly enhance the CL intensity of fluorescein (FL) in the oxidation of hydrogen peroxide catalyzed by horseradish peroxidase (HRP) [39], However, no CL enhancement was observed when anionic micelles of sodium dodecyl sulphate (SDS) or nonionic micelles of polyoxyethylene (23) dodecanol (Brij-35) were used (Fig. 9). CL enhancement is attributed to the electrostatic interaction of the anionic fluorescein with the HTAC micelles. The local concentration of fluorescein on the surface of the micelle increases the efficiency of the energy transferred from the singlet oxygen (which is produced in the peroxidation catalyzed by the HRP) to fluorescein. This chemiluminescent enhancement was applied to the determination of traces of hydrogen peroxide. The detection limit was three times smaller than that obtained in aqueous solution. [Pg.298]

Research has also been conducted in which steryl phosphonic acid (SPA) was examined in place of benzyl arsonic acid (BAA), which was used in an operating plant in China [5], In this study, several collectors were examined, including sodium laurate, sodium dodecyl sulphate, amino acids, diphosphonic acid (SPA). It was discovered that SPA was the most effective and that aliphatic alcohol (i.e. octanol) was required to maintain the effectiveness of SPA. The use of emulsifier in the mixture was required to provide a suitable emulsion of the composite collector. [Pg.181]

Figure 10.12 Micelles of sodium dodecyl sulphate (SDS) comprise as many as 80 monomer units. The micelle interior comprises the hydrocarbon chains, and is oil like. The periphery presented to the water of solution is made up of hydrated hydrophilic sulphonic acid groups...

While studying the binding of mercury by chromatin of rats injected with mercuric chloride, the nonhistone chromatin proteins in rat and kidney cell nuclei were shown to be mainly responsible for the mercury deposition [43]. The mercury-binding nonhistone proteins were found to be heterogeneous by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. [Pg.197]

There is a recent trend towards simultaneous CE separations of several classes of food additives. This has so far been applied to soft drinks and preserved fruits, but could also be used for other food products. An MEKC method was published (Lin et al., 2000) for simultaneous separation of intense sweeteners (dulcin, aspartame, saccharin and acesulfame K) and some preservatives (sorbic and benzoic acids, sodium dehydroacetate, methyl-, ethyl-, propyl- and isopropyl- p-hydroxybenzoates) in preserved fruits. Ion pair extraction and SPE cleanup were used prior to CE analysis. The average recovery of these various additives was 90% with good within-laboratory reproducibility of results. Another procedure was described by Frazier et al. (2000b) for separation of intense sweeteners, preservatives and colours as well as caffeine and caramel in soft drinks. Using the MEKC mode, separation was obtained in 15 min. The aqueous phase was 20 mM carbonate buffer at pH 9.5 and the micellar phase was 62 mM sodium dodecyl sulphate. A diode array detector was used for quantification in the range 190-600 nm, and limits of quantification of 0.01 mg/1 per analyte were reported. The authors observed that their procedure requires further validation for quantitative analysis. [Pg.125]

Figure 6.25 Plot of the normalised extensional viscosity of an 8% PVP solution with 320 mM sodium dodecyl sulphate. The data is plotted versus the reduced extension rate...

An interesting extension of aqueous solution radiolysis involved solutions of sodium dodecyl sulphate in the presence of MNP. Spin adducts of secondary alkyl radicals were detected provided that the critical micelle concentration of the surfactant was exceeded. Whilst it was rather loosely concluded that there is a marked catalytic effect of micelles on the rates of reaction of radicals with nitroso spin traps , no single origin of this effect could be clearly identified (Bakalik and Thomas, 1977). [Pg.40]

Procedure Dissolve accurately weighed 1 mg of corticotophin in 1 ml of 1 M acetic acid containing 1% w/v of sodium dodecyl sulphate. Heat the solution at 100 °C for 10 minutes and allow to cool. [Pg.480]

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