Abstract

Imaging of cell nuclei plays a critical role in cancer diagnosis and prognosis. To image noninvasively cell nuclei in vivo without staining, we developed UV photoacoustic microscopy (UV-PAM), in which $266\mathrm{nm}$ wavelength UV light excites unlabeled DNA and RNA in cell nuclei to produce photoacoustic waves. We applied UV-PAM to ex vivo imaging of cell nuclei in a mouse lip and a mouse small intestine and to in vivo imaging of the cell nuclei in the mouse skin. The UV-PAM images of unstained cell nuclei match the optical micrographs of the histologically stained cell nuclei. Given intrinsic optical contrast and high spatial resolution, in vivo label-free UV-PAM has potential for unique biological and clinical application.

© 2010 Optical Society of America

Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

Chi Zhang, Konstantin Maslov, and Lihong V. Wang
Opt. Lett. 35(19) 3195-3197 (2010)

Label-free cell nuclear imaging by Grüneisen relaxation photoacoustic microscopy

Xiaowei Liu, Terence T. W. Wong, Junhui Shi, Jun Ma, Qing Yang, and Lihong V. Wang
Opt. Lett. 43(4) 947-950 (2018)

PAExM: label-free hyper-resolution photoacoustic expansion microscopy

Hyojin Kim, Jin Woo Baik, Seungwan Jeon, Jin Young Kim, and Chulhong Kim
Opt. Lett. 45(24) 6755-6758 (2020)

Label-free in vivo fiber-based optical-resolution photoacoustic microscopy

P. Hajireza, W. Shi, and R. J. Zemp
Opt. Lett. 36(20) 4107-4109 (2011)

In vivo evaluation of a lipopolysaccharide-induced ear vascular leakage model in mice using photoacoustic microscopy

Zhe Li, Pengbo He, Yuqing Xu, Yuxiao Deng, Yuan Gao, and Sung-Liang Chen
Biomed. Opt. Express 13(9) 4802-4816 (2022)