Kinetics of Germination of Individual Spores of Geobacillus stearothermophilus as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Figure 1
Raman spectra of individual spores. Raman spectra of individual G. stearothermophilus (A), B. subtilis (B), and B. cereus spores (C), measured at 25°C (curve a), 65°C (curve b) and 95°C (curve c), respectively.
Curve d in Fig. 1(C) is the Raman spectrum of single B. cereus spores that had lost their CaDPA at 95°C. All the spectra were averages from 30 individual spores determined as described in Methods. The dotted lines are the protein bands of amide I (1653/1667 cm−1) and amide III (1253 cm−1), respectively.