Microbial metabolite deoxycholic acid shapes microbiota against Campylobacter jejuni chicken colonization
Fig 3
Bile acids fail to consistently inhibit C. jejuni in vitro growth.
C. jejuni 81–176 or AR101 at 103 CFU was inoculated into 5 ml Campylobacter broth under microaerophilic condition and cultured at 42°C for 24 hours. The broth was supplemented with various concentrations (0, 5, or 25 mM) of secondary bile acid DCA, primary bile acid CA, or conjugated primary bile acid TCA. (A) Bile acids on 81–176 growth. (B) Bile acids on AR101 growth. Different letters represent significance (P<0.05) between treatments. Results are representative of 3 independent experiments.