We developed a polarization-independent and reserved-band-less complementary spectral inverted optical phase conjugation (CSI-OPC) device using dual-band difference frequency generation based on highly efficient periodically poled LiNbO₃ waveguide technologies. To examine the nonlinearity mitigation in a long-haul transmission using a large number of OPCs, we installed a CSI-OPC device in the middle of a pure silica core fiber-based recirculating loop transmission line with a length of 320km. First, we examined the fiber-input power tolerance after 5,120-km and 6,400-km transmission using 22.5-Gbaud PDM-16QAM 10-channel DWDM signals and found a Q-factor improvement of over 1.3dB along with enhanced power tolerance thanks to mitigating the fiber nonlinearity. We then demonstrated transmission distance extension using the CSI-OPC device. The use of multiple CSI-OPCs enables an obvious performance improvements attained by extending the transmission distance from 6,400km to 8,960km, which corresponds to applying the CSI-OPC device 28 times. Moreover, there was no Q-factor degradation for the link in a linear regime after applying the CSI-OPC device more than 16 times. These results demonstrate that the CSI-OPC device can improve the nonlinear tolerance of PDM-16QAM signals without an excess penalty.