In this study we propose a new image enhancement technique named Dynamic Band Imaging (DBI) which changes its parameters in time. DBI is the technology based on multi-spectral estimation to enhance the endoscopic color images in order to distinguish the slight color difference more clearly. From some results of fundamental analysis by subjective evaluations, DBI represents the effectiveness for image enhancement in comparison with the typical still image evaluation. Furthermore we developed the user interface to determine many parameters easily and then some practical appropriate parameters for endoscopic diagnosis are obtained. DBI is found as the effective method to enhance medical images.