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Chagas disease

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Chagas disease (Template:Lang-pt, Template:Lang-es, mal de Chagas in both languages; also called American trypanosomiasis) is a tropical parasitic disease caused by the flagellate protozoan Trypanosoma cruzi. T. cruzi is commonly transmitted to humans and other mammals by an insect vector, the blood-sucking insects of the subfamily Triatominae (family Reduviidae) most commonly species belonging to the Triatoma, Rhodnius, and Panstrongylus genera.[1] The disease may also be spread through blood transfusion and organ transplantation, ingestion of food contaminated with parasites, and from a mother to her fetus.[2]

Chagas disease
SpecialtyInfectious diseases, parasitology Edit this on Wikidata

The symptoms of Chagas disease vary over the course of an infection. In the early, acute stage, symptoms are mild and usually produce no more than local swelling at the site of infection. The initial acute phase is responsive to antiparasitic treatments, with 60–90% cure rates. After 4–8 weeks, individuals with active infections enter the chronic phase of Chagas disease that is asymptomatic for 60–80% of chronically infected individuals through their lifetime. The antiparasitic treatments also appear to delay or prevent the development of disease symptoms during the chronic phase of the disease, but 20–40% of chronically infected individuals will still eventually develop life-threatening heart and digestive system disorders. The currently available antiparasitic treatments for Chagas disease are benznidazole and nifurtimox, which can cause temporary side effects in many patients including skin disorders, brain toxicity, and digestive system irritation.[3][4][5]

Chagas disease is contracted primarily in the Americas, particularly in poor, rural areas of Mexico, Central America, and South America; very rarely, the disease has originated in the Southern United States. The insects that spread the disease are known by various local names, including vinchuca in Argentina, Bolivia, Chile and Paraguay, barbeiro (the barber) in Brazil, pito in Colombia, chinche in Central America, chipo in Venezuela, chupança, chinchorro, and "the kissing bug". It is estimated that as many as 8 to 11 million people in Mexico, Central America, and South America have Chagas disease, most of whom do not know they are infected. Large-scale population movements from rural to urban areas of Latin America and to other regions of the world have increased the geographic distribution of Chagas disease, and cases have been noted in many countries, particularly in Europe.[4] Control strategies have mostly focused on eliminating the triatomine insect vector and preventing transmission from other sources.[1]

Signs and symptoms

 
Gross anatomy of a heart that has been damaged by chronic Chagas disease. See also: Chagas heart, radiology

The human disease occurs in two stages: an acute stage, which occurs shortly after an initial infection, and a chronic stage that develops over many years.

The acute phase lasts for the first few weeks or months of infection. It usually occurs unnoticed because it is symptom free or exhibits only mild symptoms that are not unique to Chagas disease. These can include fever, fatigue, body aches, headache, rash, loss of appetite, diarrhea, and vomiting. The signs on physical examination can include mild enlargement of the liver or spleen, swollen glands, and local swelling (a chagoma) where the parasite entered the body. The most recognized marker of acute Chagas disease is called Romaña's sign, which includes swelling of the eyelids on the side of the face near the bite wound or where the bug feces were deposited or accidentally rubbed into the eye. Rarely, young children, or adults may die from the acute disease due to severe inflammation/infection of the heart muscle (myocarditis) or brain (meningoencephalitis).[6] The acute phase also can be severe in people with weakened immune systems.[1]

If symptoms develop during the acute phase, they usually resolve spontaneously within 3–8 weeks in approximately 90% of individuals.[3][4] Although the symptoms resolve, even with treatment the infection persists and enters a chronic phase. Of individuals with chronic Chagas disease, 60–80% will never develop symptoms (called indeterminate chronic Chagas disease), while the remaining 20–40% will develop life-threatening heart and/or digestive disorders during their lifetime (called determinate chronic Chagas disease). In 10% of individuals the disease progresses directly from the acute form to a symptomatic clinical form of chronic Chagas disease.[3][4]

The symptomatic (determinate) chronic stage affects the nervous system, digestive system and heart. About two thirds of people with chronic symptoms have cardiac damage, including dilated cardiomyopathy, which causes heart rhythm abnormalities and may result in sudden death. About one third of patients go on to develop digestive system damage, resulting in dilation of the digestive tract (megacolon and megaesophagus), accompanied by severe weight loss. Swallowing difficulties (secondary achalasia) may be the first symptom of digestive disturbances and may lead to malnutrition.[2] Twenty to fifty percent of individuals with intestinal involvement also exhibit cardiac involvement.[2] Up to 10% of chronically infected individuals develop neuritis that results in altered tendon reflexes and sensory impairment. Isolated cases exhibit central nervous system involvement, including dementia, confusion, chronic encephalopathy and sensitivity and motor deficits.[7]

The clinical manifestations of Chagas disease are due to cell death in the target tissues that occurs during the infective cycle, by sequentially inducing an inflammatory response, cellular lesions, and fibrosis. For example, intracellular amastigotes destroy the intramural neurons of the autonomic nervous system in the intestine and heart, leading to megaintestine and heart aneurysms, respectively. If left untreated, Chagas disease can be fatal, in most cases due to heart muscle damage.[2]

Transmission

 
Rhodnius prolixus is the principal vector in Colombia, Venezuela, Guatemala, Honduras and some parts of Nicaragua and El Salvador.

In Chagas-endemic areas, the main mode of transmission is through an insect vector called a triatomine bug.[1] A triatomine becomes infected with T. cruzi by feeding on the blood of an infected person or animal. During the day, triatomines hide in crevices in the walls and roofs. The bugs emerge at night, when the inhabitants are sleeping. Because they tend to feed on people’s faces, triatomine bugs are also known as “kissing bugs.” After they bite and ingest blood, they defecate on the person. Triatomines pass T. cruzi parasites (called trypomastigotes) in feces left near the site of the bite wound. Scratching the site of the bite causes the trypomastigotes to enter the host through the wound, or through intact mucous membranes, such as the conjunctiva. Once inside the host, the trypomastigotes invade cells, where they differentiate into intracellular amastigotes. The amastigotes multiply by binary fission and differentiate into trypomastigotes, which are then released into the bloodstream. This cycle is repeated in each newly infected cell. Replication resumes only when the parasites enter another cell or are ingested by another vector.[1] (See also: Life cycle and transmission of T. cruzi)

Dense vegetation (such as that of tropical rainforests) and urban habitats are not ideal for the establishment of the human transmission cycle. However, in regions where the sylvatic habitat and its fauna are thinned by economic exploitation and human habitation, such as in newly deforested areas, piassava palm culture areas, and some parts of the Amazon region, a human transmission cycle may develop as the insects search for new food sources.[8]

T. cruzi can also be transmitted through blood transfusions. With the exception of blood derivatives (such as fractionated antibodies), all blood components are infective. The parasite remains viable at 4 °C for at least 18 days or up to 250 days when kept at room temperature. It is unclear whether T. cruzi can be transmitted through frozen-thawed blood components.[9]

Other modes of transmission include organ transplantation, through breast milk,[10] and by accidental laboratory exposure. Chagas disease can also be spread congenitally (from a pregnant woman to her baby) through the placenta, and accounts for approximately 13% of stillborn deaths in parts of Brazil.[11]

In 1991, farm workers in the state of Paraíba, Brazil, were infected by eating contaminated food; transmission has also occurred via contaminated açaí palm fruit juice and sugar cane juice.[12][13][14] A 2007 outbreak in 103 Venezuelan school children was attributed to contaminated guava juice.[15]

Diagnosis

 
Photomicrograph of Giemsa-stained Trypanosoma cruzi (CDC)

The presence of T. cruzi is diagnostic of Chagas disease. It can be detected by microscopic examination of fresh anticoagulated blood, or its buffy coat, for motile parasites; or by preparation of thin and thick blood smears stained with Giemsa, for direct visualization of parasites. Microscopically, T. cruzi can be confused with Trypanosoma rangeli, which is not known to be pathogenic in humans. Isolation of T. cruzi can occur by inoculation into mice, by culture in specialized media (for example,, NNN, LIT); and by xenodiagnosis,[16] where uninfected Reduviidae bugs are fed on the patient's blood, and their gut contents examined for parasites.[2]

Various immunoassays for T. cruzi are available and can be used to distinguish among strains (zymodemes of T.cruzi with divergent pathogenicities). These tests include: detecting complement fixation, indirect hemagglutination, indirect fluorescence assays, radioimmunoassays, and ELISA. Alternatively, diagnosis and strain identification can be made using polymerase chain reaction (PCR).[2]

Prevention

 
Awareness and prevention campaign poster in Cayenne, French Guiana, 2008

There is currently no vaccine against Chagas disease[17] and prevention is generally focused on fighting the vector Triatoma by using sprays and paints containing insecticides (synthetic pyrethroids), and improving housing and sanitary conditions in rural areas.[18] For urban dwellers, spending vacations and camping out in the wilderness or sleeping at hostels or mud houses in endemic areas can be dangerous; a mosquito net is recommended. Some stepstones of vector control include:

  • A yeast trap tested for monitoring infestations of certain species of triatomine bugs (Triatoma sordida, Triatoma brasiliensis, Triatoma pseudomaculata, and Panstrongylus megistus).[19]
  • Promising results were gained with the treatment of vector habitats with the fungus Beauveria bassiana.[20]
  • Targeting the symbionts of Triatominae through paratransgenesis.[21]

A number of potential vaccines are currently being tested. Vaccination with Trypanosoma rangeli has produced positive results in animal models.[22] More recently, the potential of DNA vaccines for immunotherapy of acute and chronic Chagas disease is being tested by several research groups.[23]

Blood transfusion was formerly the second most common mode of transmission for Chagas disease, but the development and implementation of blood bank screening tests has dramatically reduced this risk in the last decade. Blood donations in all endemic Latin American countries undergo Chagas screening, and testing is expanding in countries, such as France, Spain and the United States, that have significant or growing populations of immigrants from endemic areas.[24][25] In Spain, donors are evaluated with a questionnaire to identify individuals at risk of Chagas exposure for screening tests.[25] The US FDA has approved two Chagas tests including one recently approved in April 2010, and has published guidelines that recommend testing of all donated blood and tissue products.[25][26] While these tests are not required in U.S., it is estimated that 75–90% of the blood supply is currently tested for Chagas, including all units collected by the American Red Cross which accounts for 40% of the U.S. blood supply.[26][27] The Chagas Biovigilance Network reports current incidents of Chagas positive blood products in the United States, as reported by labs using the screening test approved by the FDA in 2007.[28]

Management

There are two approaches to treating Chagas disease, antiparasitic treatment, to kill the parasite; and symptomatic treatment, to manage the symptoms and signs of infection.

Medication

Antiparasitic treatment is most effective early in the course of infection, but is not limited to cases in the acute phase. Drugs of choice include azole or nitro derivatives such as benznidazole[29] or nifurtimox. Both agents are limited in their capacity to effect parasitologic cure (a complete elimination of T. cruzi from the body), especially in chronically infected patients, and resistance to these drugs has been reported.[30] Studies suggest that antiparasitic treatment leads to parasitological cure in about 60–85% of adults and more than 90% of infants treated in the first year of acute phase Chagas disease. Children (age 6 to 12-years) with chronic disease have a cure rate of about 60% with benznidazole. While the rate of cure declines the longer an adult has been infected with Chagas, treatment with benznidazole has been shown to slow the onset of heart disease in adults with chronic Chagas infections.[2][4]

Treatment of chronic infection in women prior to or during pregnancy does not appear to reduce the probability the disease will be passed on to the infant. Likewise, it is unclear whether prophylactic treatment of chronic infection is beneficial in persons who will undergo immunosuppression (for example, organ transplant recipients) or in persons who are already immunosuppressed (for example, those with HIV infection).[2]

Complications

In the chronic stage, treatment involves managing the clinical manifestations of the disease. For example, pacemakers and medications for irregular heartbeats, such as the anti-arrhythmia drug amiodarone, may be life saving for some patients with chronic cardiac disease,[31] while surgery may be required for megaintestine. The disease cannot be cured in this phase, however. Chronic heart disease caused by Chagas disease is now a common reason for heart transplantation surgery. Until recently, however, Chagas disease was considered a contraindication for the procedure, since the heart damage could recur as the parasite was expected to seize the opportunity provided by the immunosuppression that follows surgery.[32] It was noted that survival rates in Chagas patients could be significantly improved by using lower dosages of the immunosuppressant drug ciclosporin. Recently, direct stem cell therapy of the heart muscle using bone marrow cell transplantation has been shown to dramatically reduce risks of heart failure in Chagas patients.[33]

Experimental treatments

Several experimental treatments have shown promise in animal models. These include inhibitors of oxidosqualene cyclase and squalene synthase,[34][35] cysteine protease inhibitors,[34][36] dermaseptins collected from frogs in the genus Phyllomedusa (P. oreades and P. distincta),[37] the sesquiterpene lactone dehydroleucodine (DhL) which affects the growth of cultured epimastigote–phase Trypanosoma cruzi,[38] inhibitors of purine uptake,[34] and inhibitors of enzymes involved in trypanothione metabolism.[39] It is hoped that new drug targets may be revealed following the sequencing of the T. cruzi genome.[40] A 2004 in vitro study suggests that components of green tea (catechins) may be effective against Trypanosoma cruzi.[41]

Epidemiology

 
Chagas in Latin America (A:Endemic zones)

Chagas disease affects 8–10 million people living in endemic Latin American countries, with an additional 300,000–400,000 living in non-endemic countries, including Spain and the United States. An estimated 41,200 new cases occur annually in endemic countries and 14,400 infants are born with congenital Chagas disease annually. About 20,000 deaths are attributed to Chagas disease each year.[2][4]

The disease is present in 18 countries on the American continents, ranging from the southern United States to northern Argentina.[1] Chagas exists in two different ecological zones. In the Southern Cone region the main vector lives in and around human homes. In Central America and Mexico the main vector species lives both inside dwellings and in uninhabited areas. In both zones Chagas occurs almost exclusively in rural areas, where triatomine breed and feed on the over 150 species from 24 families of domestic and wild mammals, as well as humans, that are the natural reservoirs of T.cruzi.[42] Although Triatominae bugs feed on birds, they appear to be immune against infection and therefore are not considered to be a T. cruzi reservoir. Even when colonies of insects are eradicated from a house and surrounding domestic animal shelters, they can re-emerge from plants or animals that are part of the ancient, sylvatic (referring to wild animals) infection cycle. This is especially likely in zones with mixed open savannah, with clumps of trees interspersed by human habitation.[43]

 
Disability-adjusted life year for chagas disease per 100,000 inhabitants in 2004.[44]
  no data
  < 10
  10-20
  20-30
  30-40
  40-50
  50-75
  75-100
  100-150
  150-200
  200-250
  250–450
  > 450

The primary wildlife reservoirs for Trypanosoma cruzi in the United States include opossums, raccoons, armadillos, squirrels, woodrats and mice.[45] Opossums are particularly important as reservoirs because the parasite can complete its life cycle in the anal glands of the animal without having to re-enter the insect vector.[45] Recorded prevalence of the disease in opossums in the U.S. ranges from 8.3%[45] up to 37.5%.[46] Studies on raccoons in the Southeast have yielded infection rates ranging from 47%[47] to as low as 15.5%.[45] Armadillo prevalence studies have been described in Louisiana and range from a low of 1.1%[46] up to 28.8%.[48] Additionally small rodents including squirrels, mice and rats are important in the sylvatic transmission cycle because of their importance as bloodmeal sources for the insect vectors. A Texas study revealed 17.3% percent T. cruzi prevalence in 75 specimens representing four separate small rodent species.[49]

Chronic Chagas disease remains a major health problem in many Latin American countries, despite the effectiveness of hygienic and preventive measures, such as eliminating the transmitting insects. However, several landmarks have been achieved in the fight against Chagas disease in Latin America including a reduction by 72% of the incidence of human infection in children and young adults in the countries of the Southern Cone Initiative, and at least three countries (Uruguay, in 1997, and Chile, in 1999, and Brazil in 2006) have been certified free of vectorial and transfusional transmission.[2][50][51] In Argentina vectorial transmission has been interrupted in 13 of the 19 endemic provinces.[50] and major progress toward this goal has also been made in both Paraguay and Bolivia.

Screening of donated blood, blood components, solid organ donors, as well as donors of cells, tissues and cell and tissue products for T. cruzi is mandated in all Chagas endemic countries and has been implemented.[52] Approximately 300,000 infected people live in the United States, which is likely the result of immigration from Latin American countries.[53] With increased population movements, the possibility of transmission by blood transfusion became more substantial in the United States. Transfusion blood and tissue products are now actively screened in the U.S., thus addressing and minimizing this risk.[54]

History

 
Carlos Chagas, in his laboratory at the Instituto Oswaldo Cruz.

The disease was named after the Brazilian physician and infectologist Carlos Chagas, who first described it in 1909,[55][56][57][58] but the disease was not seen as a major public health problem in humans until the 1960s (the outbreak of Chagas disease in Brazil in the 1920s went widely ignored[59]). He discovered that the intestines of Triatomidae (now Reduviidae: Triatominae) harbored a flagellate protozoan, a new species of the Trypanosoma genus, and was able to prove experimentally that it could be transmitted to marmoset monkeys that were bitten by the infected bug. Later studies showed that squirrel monkeys were also vulnerable to infection.[60]

Chagas named the pathogenic parasite Trypanosoma cruzi[55] and later that year as Schizotrypanum cruzi,[57] both honoring Oswaldo Cruz, the noted Brazilian physician and epidemiologist who fought successfully epidemics of yellow fever, smallpox, and bubonic plague in Rio de Janeiro and other cities in the beginning of the 20th century. Chagas’ work is unique in the history of medicine because he was the only researcher so far to describe solely and completely a new infectious disease: its pathogen, vector, host, clinical manifestations, and epidemiology.

Nevertheless, he believed (falsely) until 1925 that the main infection route was by the bite of the insect—and not by its feces, as was proposed by his colleague Emile Brumpt in 1915 and assured by Silveira Dias in 1932, Cardoso in 1938, and Brumpt himself in 1939.[citation needed] Chagas was also the first to unknowingly discover and illustrate the parasitic fungal genus Pneumocystis, later infamously to be linked to PCP (Pneumocystis pneumonia in AIDS victims).[56] Confusion between the two pathogens' life-cycles led him to briefly recognize his genus Schizotrypanum, but following the description of Pneumocystis by others as an independent genus, Chagas returned to the use of the name Trypanosoma cruzi.

In Argentina, the disease is known as Mal de Chagas-Mazza, in honor of Salvador Mazza, the Argentine physician who in 1926 began investigating the disease and over the years became the principal researcher of this disease in the country.[61] Mazza produced the first scientific confirmation of the existence of Trypanosoma cruzi in Argentina in 1927, eventually leading to support from local and European medical schools and Argentine government policy makers.[62]

It has been hypothesized that Charles Darwin might have suffered from Chagas disease as a result of a bite of the so-called Great Black Bug of the Pampas (vinchuca) (see Charles Darwin's illness). The episode was reported by Darwin in his diaries of the Voyage of the Beagle as occurring in March 1835 to the east of the Andes near Mendoza. Darwin was young and generally in good health, though six months previously he had been ill for a month near Valparaiso, but in 1837, almost a year after he returned to England, he began to suffer intermittently from a strange group of symptoms, becoming incapacitated for much of the rest of his life. Attempts to test Darwin's remains at the Westminster Abbey by using modern PCR techniques were met with a refusal by the Abbey's curator.[63]

See also

References

  1. ^ a b c d e f "DPDx – Trypanosomiasis, American. Fact Sheet". Centers for Disease Control (CDC). Retrieved 12 May 2010.
  2. ^ a b c d e f g h i j Louis V Kirchhoff (17 December 2010). "Chagas Disease (American Trypanosomiasis)". eMedicine. Retrieved 12 May 2010. {{cite web}}: Cite has empty unknown parameter: |coauthors= (help)
  3. ^ a b c Bern C, Montgomery SP, Herwaldt BL; et al. (2007). "Evaluation and treatment of chagas disease in the United States: a systematic review". JAMA. 298 (18): 2171–81. doi:10.1001/jama.298.18.2171. PMID 18000201. Retrieved 12 May 2010. {{cite journal}}: Explicit use of et al. in: |author= (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  4. ^ a b c d e f Rassi A, Rassi A, Marin-Neto JA (2010). "Chagas disease". Lancet. 375 (9723): 1388–402. doi:10.1016/S0140-6736(10)60061-X. PMID 20399979. Retrieved 12 May 2010. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  5. ^ Rassi A, Dias JC, Marin-Neto JA, Rassi A (2009). "Challenges and opportunities for primary, secondary, and tertiary prevention of Chagas' disease". Heart. 95 (7): 524–34. doi:10.1136/hrt.2008.159624. PMID 19131444. Retrieved 12 May 2010. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  6. ^ Guimarães FN, da Silva NN, Clausell DT, de Mello AL, Rapone T, Snell T, Rodrigues N (1968). "Um surto epidêmico de doença de Chagas de provável transmissão digestiva, ocorrido em Teutonia (Estrêla – Rio Grande Do Sul)". Hospital (Rio J). 73 (6): 1767–804.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  7. ^ Córdova E, Maiolo E, Corti M, Orduña T (2010). "Neurological manifestations of Chagas' disease". Neurol. Res. 32 (3): 238–44. doi:10.1179/016164110X12644252260637. PMID 20406601. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  8. ^ Teixeira AR, Monteiro PS, Rebelo JM (2001). "Emerging Chagas disease: trophic network and cycle of transmission of Trypanosoma cruzi from palm trees in the Amazon". Emerging Infect Dis. 7 (1): 100–12. doi:10.3201/eid0701.010115. PMC 2631687. PMID 11266300.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  9. ^ Wendel S (2010). "Transfusion transmitted Chagas disease: Is it really under control?". Acta Trop. 115 (1–2): 28–34. doi:10.1016/j.actatropica.2009.12.006. PMID 20044970. Retrieved 13 May 2010. {{cite journal}}: Unknown parameter |month= ignored (help)
  10. ^ Santos Ferreira C, Amato Neto V, Gakiya E, Bezerra RC, Alarcón RS (2003). "Microwave treatment of human milk to prevent transmission of Chagas disease". Revista do Instituto de Medicina Tropical de São Paulo. 45 (1): 41–2. PMID 12751321.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  11. ^ Hudson L, Turner MJ (1984). "Immunological consequences of infection and vaccination in South American trypanosomiasis [and discussion]". Philos Trans R Soc Lond, B, Biol Sci. 307 (1131): 51–61. Bibcode:1984RSPTB.307...51H. doi:10.1098/rstb.1984.0108. JSTOR 2990154. PMID 6151688. {{cite journal}}: Unknown parameter |month= ignored (help) Retrieved 22 February 2007 through JSTOR.
  12. ^ "Chagas' disease (American trypanosomiasis) in southern Brazil" (PDF). CDR Weekly. 15 (13). United Kingdom Health Protection Agency. 2005. Retrieved 26 November 2007. {{cite journal}}: Unknown parameter |month= ignored (help)
  13. ^ Shikanai-Yasuda MA, Marcondes CB, Guedes LA (1991). "Possible oral transmission of acute Chagas' disease in Brazil". Rev Inst Med Trop São Paulo. 33 (5): 351–7. PMID 1844961.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  14. ^ da Silva Valente SA, de Costa Valente V, Neto HF (1999). "Considerations on the epidemiology and transmission of Chagas disease in the Brazilian Amazon". Mem Inst Oswaldo Cruz. 94 Suppl 1: 395–8. PMID 10677763.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  15. ^ Alarcón de Noya B, Díaz-Bello Z, Colmenares C; et al. (2010). "Large urban outbreak of orally acquired acute Chagas disease at a school in Caracas, Venezuela". J Infect Dis. 201 (9): 1308–15. doi:10.1086/651608. PMID 20307205. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
  16. ^ Brumpt E (1914). "Le xénodiagnostic. Application au diagnostic de quelques infections parasitaires et en particulier à la trypanosomose de Chagas" (PDF). Bull Soc Pathol Exot. 7 (10): 706–10.
  17. ^ "A killer that preys on the poor: Chagas disease" (pdf). Médecins Sans Frontières: Activity Report 2003/2004. Retrieved 29 August 2008.
  18. ^ Eduardo N. Zerba (1999). "Susceptibility and resistance to insecticides of Chagas disease vectors" (PDF). Medicina (Buenos Aires). 59 (Suppl 2): 41–6.
  19. ^ Pires HH, Lazzari CR, Diotaiuti L, Lorenzo MG (2000). "Performance of yeast-baited traps with Triatoma sordida, Triatoma brasiliensis, Triatoma pseudomaculata, and Panstrongylus megistus in laboratory assays". Rev Panam Salud Publica. 7 (6): 384–8. doi:10.1590/S1020-49892000000600005. PMID 10949899. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  20. ^ Luz C, Rocha LF, Nery GV, Magalhães BP, Tigano MS (2004). "Activity of oil-formulated Beauveria bassiana against Triatoma sordida in peridomestic areas in Central Brazil". Mem Inst Oswaldo Cruz. 99 (2): 211–8. doi:10.1590/S0074-02762004000200017. PMID 15250478. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  21. ^ Beard CB, Cordon-Rosales C, Durvasula RV (2002). "Bacterial symbionts of the Triatominae and their potential use in control of Chagas disease transmission". Annu Rev Entomol. 47: 123–41. doi:10.1146/annurev.ento.47.091201.145144. PMID 11729071.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  22. ^ Basso B, Moretti E, Fretes R (2008). "Vaccination with epimastigotes of different strains ofTrypanosoma rangeli protects mice against Trypanosoma cruzi infection". Mem Inst Oswaldo Cruz. 103 (4): 370–4. doi:10.1590/S0074-02762008000400010. PMID 18660992. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  23. ^ Dumonteil E, Escobedo-Ortegon J, Reyes-Rodriguez N, Arjona-Torres A, Ramirez-Sierra M (2004). "Immunotherapy of Trypanosoma cruziinfection with DNA vaccines in mice". Infect Immun. 72 (1): 46–53. doi:10.1128/IAI.72.1.46-53.2004. PMC 343959. PMID 14688079.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  24. ^ Castro E (2009). "Chagas' disease: lessons from routine donation testing". Transfus Med. 19 (1): 16–23. doi:10.1111/j.1365-3148.2009.00915.x. PMID 19302451. Retrieved 12 May 2010. {{cite journal}}: Unknown parameter |month= ignored (help)
  25. ^ a b c Gascon J, Bern C, Pinazo MJ (2009). "Chagas disease in Spain, the United States and other non-endemic countries". Acta Trop. 115 (1–2): 22–7. doi:10.1016/j.actatropica.2009.07.019. PMID 19646412. Retrieved 12 May 2010. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  26. ^ a b "FDA Approves Chagas Disease Screening Test for Blood, Tissue and Organ Donors". Retrieved 12 May 2010.
  27. ^ "Infectious Disease Testing". American Red Cross. Retrieved 12 May 2010.
  28. ^ "Chagas' Biovigilance Network". Retrieved 12 May 2010.
  29. ^ Garcia S, Ramos CO, Senra JF (2005). "Treatment with benznidazole during the chronic phase of experimental Chagas' disease decreases cardiac alterations". Antimicrob Agents Chemother. 49 (4): 1521–8. doi:10.1128/AAC.49.4.1521-1528.2005. PMC 1068607. PMID 15793134. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  30. ^ Buckner FS, Wilson AJ, White TC, Van Voorhis WC (1998). "Induction of resistance to azole drugs in Trypanosoma cruzi". Antimicrob Agents Chemother. 42 (12): 3245–50. PMC 106029. PMID 9835521. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  31. ^ Dubner S, Schapachnik E, Riera AR, Valero E (2008). "Chagas disease: state-of-the-art of diagnosis and management". Cardiol J. 15 (6): 493–504. PMID 19039752.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  32. ^ Bocchi EA, Bellotti G, Mocelin AO (1996). "Heart transplantation for chronic Chagas' heart disease". Ann Thorac Surg. 61 (6): 1727–33. doi:10.1016/0003-4975(96)00141-5. PMID 8651775. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  33. ^ Vilas-Boas F, Feitosa GS, Soares MB (2006). "Early results of bone marrow cell transplantation to the myocardium of patients with heart failure due to Chagas disease". Arq Bras Cardiol (in Portuguese). 87 (2): 159–66. PMID 16951834. {{cite journal}}: Unknown parameter |month= ignored (help); Unknown parameter |trans_title= ignored (|trans-title= suggested) (help)CS1 maint: multiple names: authors list (link)
  34. ^ a b c Scientific Working Group on Chagas Disease [April 2005] (July 2007). Guhl F, Lazdins-Helds JK (eds.): Reporte del grupo de trabajo científico sobre la enfermedad de Chagas (in Spanish). Geneva: WHO. Retrieved on 29 August 2008. Executive summary in English
  35. ^ Urbina JA, Concepcion JL, Rangel S, Visbal G, Lira R (2002). "Squalene synthase as a chemotherapeutic target in Trypanosoma cruzi and Leishmania mexicana". Mol Biochem Parasitol. 125 (1–2): 35–45. doi:10.1016/S0166-6851(02)00206-2. PMID 12467972.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  36. ^ Engel JC, Doyle PS, Hsieh I, McKerrow JH (1998). "Cysteine protease inhibitors cure an experimental Trypanosoma cruzi infection". J Exp Med. 188 (4): 725–34. doi:10.1084/jem.188.4.725. PMC 2213346. PMID 9705954. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  37. ^ Brand GD, Leite JR, Silva LP (2002). "Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta. Anti-Trypanosoma cruzi activity without cytotoxicity to mammalian cells". J Biol Chem. 277 (51): 49332–40. doi:10.1074/jbc.M209289200. PMID 12379643. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  38. ^ Brengio SD, Belmonte SA, Guerreiro E, Giordano OS, Pietrobon EO, Sosa MA (2000). "The sesquiterpene lactone dehydroleucodine (DhL) affects the growth of cultured epimastigotes of Trypanosoma cruzi". J Parasitol. 86 (2): 407–12. doi:10.1645/0022-3395(2000)086[0407:TSLDDA]2.0.CO;2. PMID 10780563. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  39. ^ Fairlamb AH, Cerami A (1992). "Metabolism and functions of trypanothione in the Kinetoplastida". Annu Rev Microbiol. 46: 695–729. doi:10.1146/annurev.mi.46.100192.003403. PMID 1444271.
  40. ^ El-Sayed NM, Myler PJ, Bartholomeu DC (2005). "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease". Science. 309 (5733): 409–15. Bibcode:2005Sci...309..409E. doi:10.1126/science.1112631. PMID 16020725. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  41. ^ Cristina Paveto, María C. Güida, Mónica I. Esteva, Virginia Martino, Jorge Coussio, Mirtha M. Flawiá, and Héctor N. Torres (January 2004). "Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins". Antimicrob Agents Chemother. 48: 69–74. doi:10.1128/AAC.48.1.69-74.2004. Retrieved 11 September 2011.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  42. ^ Morel CM, Lazdins J (2003). "Chagas disease". Nat Rev Microbiol. 1 (1): 14–5. doi:10.1038/nrmicro735. PMID 15040175. {{cite journal}}: Unknown parameter |month= ignored (help)
  43. ^ Pinto-Dias JC (1992). "Epidemiology of Chagas disease". In Wendel S, Brener Z, Camargo ME, Rassi A (eds.) (ed.). Chagas Disease—American Trypanosomiasis: its impact on transfusion and clinical medicine. ISBT Brazil '92. XXII Congress of the International Society of Blood Transfusion. XX Brazilian Congress of Hematology. Extraordinary Congress of the Brazilian College of Hematology. São Paulo: Editorial ISBT Brazil. Retrieved 11 September 2008. {{cite conference}}: |editor= has generic name (help); Unknown parameter |booktitle= ignored (|book-title= suggested) (help)CS1 maint: multiple names: editors list (link)
  44. ^ "Mortality and Burden of Disease Estimates for WHO Member States in 2004". World Health Organization.
  45. ^ a b c d Karsten V, Davis C, Kuhn R (1992). "Trypanosoma cruzi in wild raccoons and opossums in North Carolina". J Parasitol. 78 (3): 547–9. doi:10.2307/3283667. JSTOR 3283667. PMID 1597808. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  46. ^ a b Barr SC, Brown CC, Dennis VA, Klei TR (1991). "The lesions and prevalence of Trypanosoma cruzi in opossums and armadillos from southern Louisiana". J Parasitol. 77 (4): 624–7. doi:10.2307/3283170. JSTOR 3283170. PMID 1907654. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  47. ^ Yabsley MJ, Noblet GP (1 January 2002). "Seroprevalence of Trypanosoma cruzi in raccoons from South Carolina and Georgia". J Wildl Dis. 38 (1): 75–83. PMID 11838232.
  48. ^ Yaeger RG (1988). "The prevalence of Trypanosoma cruzi infection in armadillos collected at a site near New Orleans, Louisiana". Am J Trop Med Hyg. 38 (2): 323–6. PMID 3128127. {{cite journal}}: Unknown parameter |month= ignored (help)
  49. ^ Burkholder JE, Allison TC, Kelly VP (1980). "Trypanosoma cruzi (Chagas) (Protozoa: Kinetoplastida) in invertebrate, reservoir, and human hosts of the lower Rio Grande valley of Texas". J Parasitol. 66 (2): 305–11. doi:10.2307/3280824. JSTOR 3280824. PMID 6771371. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  50. ^ a b "The Southern Cone Initiative: an update". Special Programme for Research and Training in Tropical Diseases (TDR) (Press release). WHO. 2004. Retrieved 29 August 2008.
  51. ^ Massad E (2008). "The elimination of Chagas' disease from Brazil". Epidemiol Infect. 136 (9): 1153–64. doi:10.1017/S0950268807009879. PMC 2870925. PMID 18053273. {{cite journal}}: Unknown parameter |month= ignored (help)
  52. ^ Castro E (2009). "Chagas' disease: lessons from routine donation testing". Transfus Med. 19 (1): 16–23. doi:10.1111/j.1365-3148.2009.00915.x. PMID 19302451. Retrieved 13 May 2010. {{cite journal}}: Unknown parameter |month= ignored (help)
  53. ^ "Medical Encyclopedia: Chagas disease". National Institutes of Health. Retrieved 11 September 2008.
  54. ^ Kirchhoff LV; Kirchhoff, Louis V. (1993). "American trypanosomiasis (Chagas' disease)—a tropical disease now in the United States". N Engl J Med. 329 (9): 639–44. doi:10.1056/NEJM199308263290909. PMID 8341339. {{cite journal}}: Unknown parameter |month= ignored (help)
  55. ^ a b Chagas C (1909). "Neue Trypanosomen". Vorläufige Mitteilung Arch Schiff Tropenhyg. 13: 120–2.
  56. ^ a b Redhead SA, Cushion MT, Frenkel JK, Stringer JR (2006). "Pneumocystis and Trypanosoma cruzi: nomenclature and typifications". J Eukaryot Microbiol. 53 (1): 2–11. doi:10.1111/j.1550-7408.2005.00072.x. PMID 16441572.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  57. ^ a b Chagas C (1909). "Nova tripanozomiase humana: Estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homem [New human trypanosomiasis. Studies about the morphology and life-cycle of Schizotripanum cruzi, etiological agent of a new morbid entity of man]". Mem Inst Oswaldo Cruz. 1 (2): 159–218. doi:10.1590/S0074-02761909000200008. ISSN 0074-0276. (in Portuguese with German full translation as "Ueber eine neue Trypanosomiasis des Menschen.")
  58. ^ Kropf SP, Sá MR (2009 Jul). "The discovery of Trypanosoma cruzi and Chagas disease (1908–1909): tropical medicine in Brazil". Hist Cienc Saude Manguinhos. 16 (Suppl 1): 13–34. PMID 20027916. {{cite journal}}: Check date values in: |date= (help)
  59. ^ Coutinho M (1999). "Review of Historical Aspects of American Trypanosomiasis (Chagas' Disease) by Matthias Perleth". Isis. 90 (2): 397. doi:10.1086/384393. JSTOR 237120. {{cite journal}}: Unknown parameter |month= ignored (help)
  60. ^ Hulsebos LH, Choromanski L, Kuhn RE (1989). "The effect of interleukin-2 on parasitemia and myocarditis in experimental Chagas' disease". J Protozool. 36 (3): 293–8. PMID 2499678.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  61. ^ "ALCHA - Enfermedad de Chagas- Mazza ¿Cuales son sus Características?". Retrieved 15 September 2011.
  62. ^ "Historia de la enfermedad de Chagas" (in Spanish).
  63. ^ Adler D (1989). "Darwin's Illness". Isr J Med Sci. 25 (4): 218–21. PMID 2496051.

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