Kuliah Parasitoid
Kuliah Parasitoid
Kuliah Parasitoid
AGENS HAYATI
PARASITOID
NURARIATY AGUS
PERBEDAAN PARASITOID, PREDATOR DAN
ENTOMOPATOGEN
ISOLASI
PARASITOID
PERBANYAKAN
HAMA AUGMENTASI
Persiapan
Ilmu Pengetahuan :
- Taksonomi
- Biologi
- Ekologi
- Perilaku
- Seleksi parasitoid
- Metodologi
- Sistem PHT
- Analisis ekonomi
Persiapan
Teknik
- Mass rearing
- Penyimpanan
- Transportasi dan Release
- Kesesuaian habitat dan manipulasi
- Evaluasi
ISOLASI PARASITOID
KRITERIA DIDAERAH
ISOLASI EKSPLORASI :
1.Pencarian awal di daerah asal
2.Iklim dan lingkungan similar
3.Flora, fauna dan habitat beragam
EKSPLORASI 4.Cukup luas dan beragam
5.Dekat dari centre of foreign
TAHAPAN :
1. Teknik pencarian
2. Teknik koleksi
3. Teknik pengiriman
PERBANYAKAN (MASS REARING) PARASITOID
Pupa P.xylostella
(400 puPupa P.xylostella
( pa/kPupa P.xylostella
urungan) imago
instar I
4 hari
6 hari
Instar II
2 hari
3 hari
III. Perbanyakan parasitoid D. semiclausum
7 hari
\
Kokon
Diadegma semiclausum
Pada 7º (4-10oC)
Untuk pengujian
diblender
Freezer
Contoh : Perbanyakan predator burung hantu, (Tito alba )
International protocols for importing biological control agents were strictly followed. A
dossier was prepared and all necessary authorization was obtained from relevant
ministries.
5. Mass rearing of biological control agent at laboratory condition
At the laboratory provided by the management of Sun Island resort, mass rearing of the
parasitoid began upon arrival of the parasitoids. To mass rear the parasitoids, the coconut
hispid beetle was also required to be reared. Both these activities were carried out at Sun
Island by MOFAMR staff assisted by resort staff.
The rearing procedure developed by Long Nam University, Viet Nam was followed.
Following the exposure of the first generation of parasitoids (i.e. those that emerged from
the mummies imported from Viet Nam) to Brontispa host larvae for parasitization, a
representative sample (some 100 dead parasitoids preserved in a vial with 80 percent
ethanol) were sent to the Natural History Museum, London, United Kingdom, for
verification and confirmation of the identity of A. hispinarum. This follows international
protocols to ensure that the only the desired species is imported and used for mass rearing
in the recipient country.
International protocols for importing biological control agents were strictly followed. A
dossier was prepared and all necessary authorization was obtained from relevant ministries.
5. Mass rearing of biological control agent at laboratory condition
At the laboratory provided by the management of Sun Island resort, mass rearing of the
parasitoid began upon arrival of the parasitoids. To mass rear the parasitoids, the coconut
hispid beetle was also required to be reared. Both these activities were carried out at Sun
Island by MOFAMR staff assisted by resort staff.
The rearing procedure developed by Long Nam University, Viet Nam was followed.
Following the exposure of the first generation of parasitoids (i.e. those that emerged from
the mummies imported from Viet Nam) to Brontispa host larvae for parasitization, a
representative sample (some 100 dead parasitoids preserved in a vial with 80 percent
ethanol) were sent to the Natural History Museum, London, United Kingdom, for
verification and confirmation of the identity of A. hispinarum. This follows international
protocols to ensure that the only the desired species is imported and used for mass rearing
in the recipient country.