Etidijum bromid

Etidijum bromid
	3,8-Diamino-5-etil-6-fenilfenantridinijum bromid
Drugi nazivi	2,7-Diamino-10-etil-6-fenilfenantridinijum bromid, 2,7-Diamino-10-etil-9-fenilfenantridinijum bromid, 3,8-Diamino-1-etil-6-fenilfenantridinijum bromid, 5-Etil-6-fenil-fenantridin-3,8-diamin bromid, Etidijum bromid, Homidijum bromid, EtBr
Identifikacija
CAS registarski broj	1239-45-8
PubChem	14710
ChemSpider	14034
UNII	059NUO2Z1L
EINECS broj	214-984-6
KEGG	C11161
ChEBI	4883
ChEMBL	CHEMBL284328
RTECS registarski broj toksičnosti	SF7950000
ATCvet code	QP51AX06
Jmol-3D slike	Slika 1
SMILES
	CC[n+]1c2cc(ccc2c3ccc(cc3c1c4ccccc4)N)N.[Br-]
InChI
	InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H ; Kod: ZMMJGEGLRURXTF-UHFFFAOYSA-N InChI=1/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H; Kod: ZMMJGEGLRURXTF-UHFFFAOYAD
Svojstva
Molekulska formula	C21H20BrN3
Molarna masa	394,294 g/mol
Agregatno stanje	Ljubičasto-crvena čvrsta materija
Tačka topljenja	260 - 262 °C
Rastvorljivost u vodi	~ 40 g/l
Opasnost
NFPA 704	0 4 0
R-oznake	R25 R36/37/38 R46
S-oznake	S22 S24/25 S26 S36/37/39 S45 S53
Tačka paljenja	> 100 °C
	(šta je ovo?) (verifikuj) ; Ukoliko nije drugačije napomenuto, podaci se odnose na standardno stanje (25 °C, 100 kPa) materijala
	Infobox references

Etidijum bromid je interkalirajući agens koji je ćesto koristi za fluorescentno obeležavanje (bojenje nukleinskih kiseline) u molekularno biološkim laboratorijama u tehnikama poput elektroforeze na gelu. On se često obeležava sa „EtBr“, mada se ta oznaka koristi i za bromoetan. Kad se izloži ultraljubičastom svetlu, on fluorescira sa narandžastom bojom, koja postaje skoro 20-puta jača nakon vezivanja za DNK. Pod imenom homidijum, on je u širokoj upotrebi od 1950-ih u veterini za lečenje tripanozomoze kod marve, bolesti koju uzrokuju tripanozomi.^[6] Visoka učestalost otpornosti na antibiotike čini ovaj tretman nepraktičnim u nekim oblastima, gde se srodni izometamidijum hlorid takođe koristi. Etidijum bromid može da bude mutagen, karcinogen ili teratogen, mada to zavisi od organizma i okolnosti.

Reference

↑ Li Q, Cheng T, Wang Y, Bryant SH (2010). „PubChem as a public resource for drug discovery.”. Drug Discov Today 15 (23-24): 1052-7. DOI:10.1016/j.drudis.2010.10.003. PMID 20970519. edit
↑ Evan E. Bolton, Yanli Wang, Paul A. Thiessen, Stephen H. Bryant (2008). „Chapter 12 PubChem: Integrated Platform of Small Molecules and Biological Activities”. Annual Reports in Computational Chemistry 4: 217-241. DOI:10.1016/S1574-1400(08)00012-1.
↑ Hettne KM, Williams AJ, van Mulligen EM, Kleinjans J, Tkachenko V, Kors JA. (2010). „Automatic vs. manual curation of a multi-source chemical dictionary: the impact on text mining”. J Cheminform 2 (1): 3. DOI:10.1186/1758-2946-2-3. PMID 20331846. edit
↑ Joanne Wixon, Douglas Kell (2000). „Website Review: The Kyoto Encyclopedia of Genes and Genomes — KEGG”. Yeast 17 (1): 48–55. DOI:10.1002/(SICI)1097-0061(200004)17:1<48::AID-YEA2>3.0.CO;2-H.
↑ Gaulton A, Bellis LJ, Bento AP, Chambers J, Davies M, Hersey A, Light Y, McGlinchey S, Michalovich D, Al-Lazikani B, Overington JP. (2012). „ChEMBL: a large-scale bioactivity database for drug discovery”. Nucleic Acids Res 40 (Database issue): D1100-7. DOI:10.1093/nar/gkr777. PMID 21948594. edit
↑ Stevenson P, Sones KR, Gicheru MM, Mwangi EK. (1995). „Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya.”. Acta Trop. 59 (2): 257–258. DOI:10.1016/0001-706X(94)00080-K. PMID 7676909.

Literatura

Borst P (November 2005). „Ethidium DNA agarose gel electrophoresis: how it started”. IUBMB Life 57 (11): 745–7. DOI:10.1080/15216540500380855. PMID 16511967.

Povezano

Spoljašnje veze

Portal Hemija

[1] Li Q, Cheng T, Wang Y, Bryant SH (2010). „PubChem as a public resource for drug discovery.”. Drug Discov Today 15 (23-24): 1052-7. DOI:10.1016/j.drudis.2010.10.003. PMID 20970519. edit

[2] Evan E. Bolton, Yanli Wang, Paul A. Thiessen, Stephen H. Bryant (2008). „Chapter 12 PubChem: Integrated Platform of Small Molecules and Biological Activities”. Annual Reports in Computational Chemistry 4: 217-241. DOI:10.1016/S1574-1400(08)00012-1.

[3] Hettne KM, Williams AJ, van Mulligen EM, Kleinjans J, Tkachenko V, Kors JA. (2010). „Automatic vs. manual curation of a multi-source chemical dictionary: the impact on text mining”. J Cheminform 2 (1): 3. DOI:10.1186/1758-2946-2-3. PMID 20331846. edit

[4] Joanne Wixon, Douglas Kell (2000). „Website Review: The Kyoto Encyclopedia of Genes and Genomes — KEGG”. Yeast 17 (1): 48–55. DOI:10.1002/(SICI)1097-0061(200004)17:1<48::AID-YEA2>3.0.CO;2-H.

[5] Gaulton A, Bellis LJ, Bento AP, Chambers J, Davies M, Hersey A, Light Y, McGlinchey S, Michalovich D, Al-Lazikani B, Overington JP. (2012). „ChEMBL: a large-scale bioactivity database for drug discovery”. Nucleic Acids Res 40 (Database issue): D1100-7. DOI:10.1093/nar/gkr777. PMID 21948594. edit

[6] Stevenson P, Sones KR, Gicheru MM, Mwangi EK. (1995). „Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya.”. Acta Trop. 59 (2): 257–258. DOI:10.1016/0001-706X(94)00080-K. PMID 7676909.

[1]

[2]

[3]

[4]

[5]

[6]