UDP-N-acetil-2-amino-2-dezoksiglukuronat dehidrogenaza
| UDP-N-acetil-2-amino-2-dezoksiglukuronat dehidrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.335 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
UDP-N-acetil-2-amino-2-dezoksiglukuronat dehidrogenaza (EC 1.1.1.335, WlbA, WbpB) je enzim sa sistematskim imenom UDP-N-acetil-2-amino-2-dezoksi-alfa-D-glukuronat:NAD+ 3-oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- UDP-N-acetil-2-amino-2-dezoksi-alfa-D-glukuronat + NAD+ UDP-2-acetamido-2-dezoksi-alfa-D-ribo-heks-3-uluronat + NADH + H+
Ovaj enzim učestvuje u biosintetičkom putu za UDP-alfa-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-didezoksi-alfa-D-manuronska kiselina), važnom prekursoru B-band lipopolisaharida. Enzimi iz Pseudomonas aeruginosa serotipa O5 i Thermus thermophilus formiraju kompleks sa enzimom koji katalizuje sleći korak sintetičkog puta (EC 2.6.1.98, UDP-2-acetamido-2-dezoksi-ribo-heksuluronat aminotransferaza). Ovaj enzim takođe deluje kao EC 1.1.99.2 (2-hidroksiglutarat dehidrogenaza), i koristi 2-oksoglutarat proizveden enzimom EC 2.6.1.98 da regeneriše čvrsto vezani NAD+. Enzimi iz Bordetella pertussis i Chromobakterija violaceum ne vezuju NAD+ jednako čvrsto, te im nije neophodan 2-oksoglutarat da funkcionišu.
- ↑ Westman, E.L., McNally, D.J., Charchoglyan, A., Brewer, D., Field, R.A. and Lam, J.S. (2009). „Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa”. J. Biol. Chem. 284: 11854-11862. PMID 19282284.
- ↑ Larkin, A. and Imperiali, B. (2009). „Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1”. Biochemistry 48: 5446-5455. PMID 19348502.
- ↑ Thoden, J.B. and Holden, H.M. (2010). „Structural and functional studies of WlbA: A dehydrogenase involved in the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid”. Biochemistry 49: 7939-7948. PMID 20690587.
- ↑ Thoden, J.B. and Holden, H.M. (2011). „Biochemical and structural characterization of WlbA from Bordetella pertussis and Chromobacterium violaceum: enzymes required for the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid”. Biochemistry 50: 1483-1491. PMID 21241053.
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