• Pi-Pi Interaction

Pi-Pi Interaction

HPLC separation of aromatic compounds on Primesep P columns.

Schematic of Primesep P
stationary phase chemistry

SIELC Technologies has developed a new class of stationary phases, which provides three interactions with analytes:
  • Reverse-phase interaction
  • Pi-pi interaction
  • Strong cation exchange interaction

Depending on analyte properties, one, two, or all three interactions can be applied to the separation.

This stationary phase facilitates the separation of structural isomers of aromatic compounds.

Additional pi-pi interaction creates a difference in the bind state for structural isomers and often resolves critical pairs of compounds. The degree of pi-pi interaction can be adjusted by varying the amount of acetonitrile in the mobile phase.