Nanoparticle Tracking Analysis (NTA)

Presentation Nanoparticle Tracking Analysis (NTA)

Presentation

The NanoSight instrument utilizes Nanoparticle Tracking Analysis (NTA) to characterize nanoparticles from 10nm -2000nm in solution. This technology utilizes the properties of both light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of particles in liquid suspension. A fluorescence mode provides differentiation of labelled particles.

Application

Determination of particle size (10nm – 2000nm)
Concentration measurements of particles
Characterization of particle size distribution

Key words

Particle-by-particle detection and tracking, particle size, particle concentration, polydisperse samples

Staff

Platform manager : Christine Chatellard
Platform contact
Office : IBS room 264
Platform : IBS room 232

Specific Equipment

Manufacturer : Malvern
Model : NanoSight NS300
Software : NanoSight NTA 3.2
Accessorie : Syringe pump

Software Guide and operating Manuel are available near the instrument or on demand.

Samples

Only non-pathogenic biological samples are accepted. Decree of the July 18, 1994 establishing the list of biological pathogens, amended by Decrees of April 17, 1997 and June 30, 1998 (Decrees in French). The list of biological pathogens is available on the website of the IPBS.

• Sample volume requirements : 0,6 ml to 1 ml
• Concentration range : 106 – 109 particles per ml
• Bring your dilution buffer

Cost

Academics: Participation to the maintenance fees = 22 euros/ hr

Access mode and booking

Instrument allocation after user training : for users who have completed training to gain operational autonomy, time is allocated to them to perform and analyze their experiments. It is mandatory to attend the 2 hours training session before using this instrument - When needed, please ask to the platform manager

Contact the platform manager to arrange a meeting for training and to book an autonomous session.
The platform is accessible to academics and industrials

The platform manager will provide assistance for :

• experimental design
• data collection
• samples considerations
• data analysis

Follow up / acknowledgements

The user agrees to promote the obtained results by mentioning the platform in the acknowledgements in case of publication or scientific communication and to communicate the reference of the article to the responsible for the platform.

Acknowledgements in publications :
Please find below the sentence that has to be written in all publications based on our UMS platforms :
"This work used the platforms of the Grenoble Instruct-ERIC Center (ISBG : UAR 3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-0005-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB). We thank Christine Chatellard, for access to the Biophysical platform."