6 Spectro
6 Spectro
6 Spectro
Spectrophotometric Analysis
School Chem. & Chem. Tech.
Practice calculating and performing dilutions of solutions. Determine the concentration of phosphate in a water sample by spectrophotometric analysis. Construct and utilize an absorbance and calibration curve. Explore the dynamics of working with a larger group of students
BACKGROUND
Spectrophotometric Analysis and the Determination of Phosphate The Absorbance Curve The Calibration Curve
P0
%T = (
P ) 100% P0
T-Transmittance A-Absorbance
Complementary color
%T A = log( ) 100 A = bc
-molar absorptivity
b-solution path length c-molar concentration
Beer-Lambert Law
max
Absorbance
Ax=0.180,cx=?
Absorbance
0 .2
0 .1
0 .0 400
410
420
430
440
450
460
W a v e le n g t h ( n m )
PROCEDURE-- Part A.
Organizing your group