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Lec 2 - Water and Plant Cells

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Water and

plant cells

Water and plant cells


80-90% of a growing plant cell is water
This varies between types of plant cells

Carrot has 85-95% water


Wood has 35-75% water
Seeds have 5-15% water

Plant continuously absorb and lose water


Lost through the leaves
Called transpiration

Water Transpiration
The evaporation of water into the atmosphere from
the leaves and stems of plants.
It occurs chiefly at the leaves while their stomata are
open for the passage of CO2 and O2 during
photosynthesis.
Transpiration is not simply a hazard of plant life.
It is the "engine" that pulls water up from the roots
to:
supply photosynthesis (1%-2% of the total)
bring minerals from the roots for biosynthesis within leaf
cool the leaf .

Water

Hydrogen Bonds
The Amazing Hydrogen Bond
results from the partial charges
generated by the 105 degree angle
between the two hydrogen atoms on
the water molecule.
Hydrogen bond: attraction of
hydrogen with a partial positive
charge to another molecule with a
partial negative charge.

Water is Polar
Water is a polar molecule with differing
charges on differing sides.
This sets up a critical difference between
molecules:
Hydrophobic--afraid of water
Hydrophilic--love water

Water
(A) Hydrogen
bonds between
water molecules
results in local
aggregations of
water molecules

(B) Theses are


very short lived,
break up rapidly to
form more random
configurations
Due to temperature
variations in water

Properties of Water
1. Water is Liquid at
Room Temperature.

2. Water is the Universal


Solvent.

Structure = Charge
Charge = Hbond

Water dissolves polar


molecules
Salts such as sodium chloride (NaCl),
dissolve in water by dissociating as each
ion becomes surrounded by the polar water
molecules

Water allows chemical


reactions to occur
Water establishes
boundaries
Remember Hydrophilic and
Hydrophobic?

Properties of Water
3. Water is Cohesive
Water sticks to sides
Water fills vessels
Water molecules cling,
but also rearrange, this
allows water to flow!

4. The temperature of
water rises and falls
slowly
This prevents sudden or
dramatic changes!
One calorie will raise 1g
of water 1 degree C.
This is over two times the
amount of energy
required for other
covalent molecules!
Protection and Buffer

Properties of Water
5. Water has a high heat
of Vaporization
Water to steam requires a
lot of energy
This property helps
moderate the earths
temperature
Life as we know it would
not exist if we were all
gasses!

6. Frozen water is less


dense than liquid water
Lakes freeze from the top
down
Allows for ice activities
Protects organisms in
Lakes

Water transport processes


Moves from soil, through plant, and to atmosphere
by a variety of mediums

Cell wall
Cytoplasm
Plasma membranes
Air spaces

How water moves depends on what it is passing


through
(Soil , plant and atmosphere continuum) - SPAC

Water across plant membranes


There is some diffusion of
water directly across the bilipid membrane.
Aquaporins: Integral
membrane proteins that form
water selective channels
allows water to diffuse faster
Facilitates water movement in
plants

Alters the rate of water flow


across the plant cell
membrane NOT direction

Permeability and Diffusion


The plasma membrane is
selectively permeable.
This means that only some
molecules can cross.
Small uncharged molecules
like O2, CO2 and H2O pass.
Large or charged molecules
like proteins or ions
cannot pass.

Diffusion is the movement


of molecules from an area
of high concentration to an
area of low concentration.

20

10
15

15

Diffusion

Diffusion works down a concentration gradient. Leads to the gradual


mixing of molecules & eventual dissipation of conc. Differences.
It is rapid over short distances, but extremely slow over long distances

Pressure-driven bulk flow drives


long-distance water transport
Bulk flow:
Concerted movement of groups of molecules, most often in
response to a pressure gradient.

Dependant on the radius of the tube that water is traveling


in.
Double radius flow rate increases 16 times!!!!!!!!!!

This is the main method for water movement in :


Xylem, Cell Walls and in the Soil.
Independent of solute concentration gradients to a point
So different from diffusion

Cell water potential


All living things need a continuous input of
free energy to maintain and repair structures,
as well as to grow and reproduce
Biochemical reactions, solute accumulation,
and long distance transport are all driven by
the input of free energy into the plant
This is defined as Water Potential.

Cell water potential - yw

The equation yw = ys + yp + yg
Affected by three factors:

ys : Solute potential or osmotic potential


The effect of dissolved solutes on water and the cell

yp : Hydrostatic pressure of the solution.


A +ve pressure is known as Turgor pressure
Can be ve, as in the xylem and cell wall this is important in
moving water long distances in plants

yg : Gravity - causes water to move downwards unless


opposed by an equal and opposite force

Osmosis and Tonicity


Osmosis is the diffusion of
water across a plasma
membrane.
Osmosis occurs when there
is an unequal concentration
of water on either side of
the selectively permeable
plasma membrane.
Remember, H2O
CAN cross the plasma
membrane.

Tonicity is the
osmolarity of a
solution--the amount of
solute in a solution.
Solute--dissolved
substances like sugars
and salts.
Tonicity is always in
comparison to a cell.
The cell has a specific
amount of sugar and
salt.

Tonic Solutions
A Hypertonic solution has more solute than
the cell. A cell placed in this solution will give
up water (osmosis) and shrink.
A Hypotonic solution has less solute than the
cell. A cell placed in this solution will take up
water (osmosis) and blow up.
An Isotonic solution has just the right amount
of solute for the cell. A cell placed in this
solution will stay the same.

Plant cell in hypotonic solution


Flaccid cell in 0.1M sucrose
solution.
Water moves from sucrose
solution to cell swells up
becomes turgid.
This is a Hypotonic solution has less solute than the cell.
So higher water concentration.
Pressure increases on the
cell wall as cell expands to
equilibrium

The equation

yw = ys + yp

ys : Solute potential or osmotic potential


The effect of dissolved solutes on water and the cell
yp : Hydrostatic pressure of the solution.
A +ve pressure is known as Turgor pressure
Can be ve, as in the xylem and cell wall this is
important in moving water long distances in plants

Plant cell in hypertonic solution


Turgid cell in 0.3M sucrose
solution
Water movers from cell to
sucrose solution
A Hypertonic solution has
more solute than the cell. So
lower water concentration.
Turgor pressure reduced and
protoplast pulls away from
the cell wall

Yw and water status of


plants
Water potential has two main uses
1: Governs water transport across membranes.
2: Uses as a measure of the water status of plant.

Because of water loss to the atmosphere plants are


seldom fully hydrated.
They suffer from water deficits
Leads to inhibition of
1. Plant growth most likely to be affected
2. Photosynthesis

Yw and water status of


plants
Cell division slows down
Reduction of synthesis of:
Cell wall
Proteins
Closure of stomata
Due to accumulation of the
plant hormone Abscisic acid
This hormone induces
closure of stomata during
water stress
Naturally more of abscisic
acid hormone in desert
plants

Summary
Water is important to plants
Makes up the media in which all biochemical processes
occur that are essential to plant life.
Influences the structure and function of proteins, cell
membranes, nucleic acids, & carbohydrates

Water movement driven by free energy. Moves


by
Osmosis, bulk flow, diffusion or a combination
Help moves water from soil through plant to atmosphere

Water potential is a measure of water status of a


plant.

Quiz 1

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