Technical Specifications BD Facsarray™

23-8382-00 FACSArray Tech Spec.
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BD Biosciences
Technical Specifications
BD FACSArray
Introduction
The BD FACSArray bioanalyzer sets a new standard for
fast and sensitive high-content analysis of applications in
cell biology, immunology, and drug discovery. The system
is compact, easy to use, and particularly suited for screening
applications in cellular and multiplex bead assays.
Speed is built in by combining a new micro-plate
technology for sample input and digital electronics
supporting acquisition rates of up to 15,000 events per
second. The optical platform of the bioanalyzer is equipped
with a green (532 nm) and a red (635 nm) laser, detecting
two particle-scattered lights, and four fluorescent parameters.
For more information about the BD FACSArray bioanalyzer
or other quality products from BD Biosciences, please contact
your local sales representative or call one of the locations
listed below.
System Performance
Excitation Optics
Fluorescence Sensitivity
Optical Platform
Mean or median differences of 200 PE molecules can be

detected between sample populations.
Fixed optical assembly

Lasers
Fluorescence Resolution
CVs of the brightest Spherotech Rainbow particles
(BD Biosciences Cat. No. 556291) are less than or equal to
7% at a sample flow rate of 1 L/sec.
Forward and Side Light Scatter Sensitivity
532 nm green solid state; 10 mW

635 nm red diode; 10 mW
Laser Beam Geometry (Both Lasers)
Focused beam height is 20 m and beam width is
65 m (nominal)
Sensitivity and dynamic range enable identification of

2.5-m to 50-m beads.
Forward and Side Light Scatter Resolution
Scatter performance resolves lymphocytes, monocytes, and
granulocytes.
Sample Acquisition Rate
Maximum acquisition rate: up to 15,000 events per second
with 6 parameters.
BD Biosciences
bdbiosciences.com
United States Canada
Europe
Japan
Asia Pacific
Latin America/Caribbean
877.232.8995 888.259.0187 32.53.720.211 0120.8555.90
65.6861.0633 55.11.5185.9995
For country-specific contact information, visit bdbiosciences.com/how_to_order/international_offices.shtml
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Emission Optics
Plate Sampler
Optical Coupling
Sample Introduction
Quartz cuvette is coupled to the emission lens by refractive

indexmatching optical gel for optimum collection efficiency.
96-well plate format
Quartz Optical Cuvette

180 m x 430 m
Forward Light Scatter Detector and Filters
High-performance solid state silicon diode detector, preceded
by a 532 nm bandpass filter for clear signal detection and
rejection of red diode laser signal.
Side Light Scatter Detector
Sensitive detection using photomultiplier and optical filters in
the emission optical bench train.
Fluorescence Detectors and Filters
Wide dynamic range photomultipliers (Yellow and Red
parameters) and avalanche photodiodes (Far Red and Near
Infrared parameters) with filters. Filters and mirrors are not
user changeable.
Two Collection Parameters from the 532 nm Laser
Yellow parameter; 585/42 nm for PE Far Red parameter
>685 nm for PE-Cy7 or PerCP-Cy5.5
Two Collection Parameters from the 635 nm Laser
Red parameter; 661/16 nm for APC or Alexa Fluor 647
Near Infrared (NIR) parameter; 780/60 nm for APC-Cy7
Sample Volume Injected

5 to 100 L (plus 20 L of overhead)
Sample Mixing
The sample of each well is mixed prior to acquisition via
uptake and resuspension of a specified mixing volume. The
mixing volume, mixing speed, and number of mixes are
customizable.
Throughput for BD CBA Ready-to-Use Kit Applications
<
_ 35 min per 96-well plate using BD CBA beads
1200 beads saved per file

300 beads/L concentration
Sample rate of 2 L/sec
Prepared in MultiScreen 96-well filtration and assay
plates
Throughput for Cell-Based Applications

<
_ 35 min per 96-well plate using lysed whole human blood
1000 events saved per file

600 cells/L concentration
Sample rate of 2 L/sec
BD Falcon 96-well polypropylene V-bottom plate
(Cat. No. 353263)
Carryover
<0.5% for bead and cell-based applications
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Fluidics
Data Management System
General Operation
Workstation
Internal fluidic tanks provide sheath fluid to the instrument

and receive waste. Sheath fluid is pumped from the
unpressurized user-accessed sheath tank to an internal
pressurized reservoir that supplies sheath fluid to the optical
cuvette and plate sampler.
PC workstation with Intel Pentium 4 at

3.0 GHz or faster
Fluidic Reservoirs
Data Storage
4-L sheath and waste containers provided with integrated

level sensing system.
80 GB hard drive CD ROM RW drive

Floppy drive 1.44 M
Sample Injection Rates
Networking
Adjustable rate from 0.5 to 3.0 L/sec
Ethernet
Preset Maintenance Options
Monitor
Start Up Fluidics
Shut Down Fluidics
Purge Sheath Filter
Drain and Fill Optical Cuvette
Clean (Daily Clean and System Clean [monthly])
Several LCD monitor sizes are available. More information

available upon request.
Memory
1 GB RAM
Printer
Ethernet supported network-ready color printers are
available. More information available upon request.
Sheath Fluid
BD FACS sheath solution with surfactant is required to
guarantee optimal and reproducible performance of the
bioanalyzer system
(Cat. No. 336524).
Data File Structure

Flow Cytometry Standard (FCS) 3.0 or 2.0
Data export formats support CSV, tab delimited, FCS list
mode
Software
Signal Processing
Workstation Display
BD FACSArray system software

FCAP Array software
Linear or four decades logarithmic display of data in

histograms or dot plots
Data Acquisition Parameters
Six digital parameters: four fluorescent and two light scatter
parameters
Fluorescent Compensation (Optical Spillover)
No limit to inter- and intra-beam compensation. Data are
acquired uncompensated to eliminate data collection
variables. Compensation is performed post acquisition during
data analysis.
10
Pulse Processing
Area and width measurements are available for the FSC and
SSC parameter. Area measurements are available for all four
fluorescent parameters. All measurement parameters acquired
as high-resolution, 18-bit, linear data for accurate postacquisition analysis.
NIR-A
10
10
10
Time
Time available correlated to any parameter.
10
Parameter Threshold
Up to two parameters
10
Red-A
10
10
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Installation Requirements
Power
Operation at 100-250 VAC, 15 Amp, and 50/60 Hz
Size and Weight
BD FACSArray bioanalyzer (excluding peripherals): 25.3
inches (64.3 cm) depth, 22.1 inches (56.1 cm) width, and
19.6 inches (49.8 cm) height; approximately 85 lb
Regulatory Requirements
CE marked for electrical safety, Europe
UL Standard for Safety of Electrical Laboratory Equipment
CSA for electrical safety, Canada
Laser safety for Class 1 (I) per applicable regulation
Technical Assistance
BD Biosciences Customer Support Center
2350 Qume Drive
San Jose, CA 95131-1807 USA
(877) 232-8995
Customers outside the US, contact your local
BD Biosciences representative or distributor.
The BD FACSArray bioanalyzer is For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Class 1 (I) Laser Product
BD, the BD Logo, BD FACS, BD Falcon, and BD FACSArray are trademarks of Becton, Dickinson and Company.
FCAP Array is a registered trademark of Soft Flow, Inc. Windows is a registered trademark of Microsoft
Corporation. Intel and Pentium are registered trademarks of Intel Corporation. MultiScreen is a registered
trademark of Millipore Corporation. Alexa Fluor is a registered trademark of Invitrogen, Inc. 2005 BD
Visit us at BDBiosciences.com/bdfacsarray
*Patent pending

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23-8382-00 FACSArray Tech Spec.

Mean or median differences of 200 PE molecules can be

Fixed optical assembly

532 nm green solid state; 10 mW

Sensitivity and dynamic range enable identification of

23-8382-00 FACSArray Tech Spec.qxp

Quartz cuvette is coupled to the emission lens by refractive

96-well plate format

Quartz Optical Cuvette

Sample Volume Injected

1200 beads saved per file

Throughput for Cell-Based Applications

1000 events saved per file

23-8382-00 FACSArray Tech Spec.qxp

Data Management System

Internal fluidic tanks provide sheath fluid to the instrument

PC workstation with Intel Pentium 4 at

4-L sheath and waste containers provided with integrated

80 GB hard drive CD ROM RW drive

Sample Injection Rates

Adjustable rate from 0.5 to 3.0 L/sec

Preset Maintenance Options

Several LCD monitor sizes are available. More information

Data File Structure

BD FACSArray system software

Linear or four decades logarithmic display of data in

23-8382-00 FACSArray Tech Spec.qxp

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