Chapter 17: From Gene To Protein
Chapter 17: From Gene To Protein
Chapter 17: From Gene To Protein
What situation did Archibald Garrod suggest caused inborn errors of metabolism?
3.
4.
State the hypothesis formulated by George Beadle while studying eye color mutations in
Drosophila.
5.
What strategy did Beadle and Tatum adopt to test this hypothesis?
6.
Which organism did Beadle and Tatum use in their research? How did this organisms
nutritional requirements facilitate this research?
7.
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8.
Study Figure 17.2 carefully. On the figure below, outline the technique used to identify and
isolate mutant fungi.
9.
Cite two significant findings that resulted from the research of Beadle and Tatum.
10.
What revision of detail (but not of basic principle) did this hypothesis undergo as more
information was gained? Write this restatement and then box or highlight it. This is an
important concept!
From the first paragraph in this section, find three ways in which RNA differs from DNA.
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12.
13.
Define each of these processes that are essential to the formation of a protein:
transcription
translation
14.
Product Synthesized
Transcription
Translation
15.
16.
Write the central dogma of molecular genetics, as proclaimed by Francis Crick, in the box
below.
17.
How many nucleotide bases are there? _______________ How many amino acids?
__________
18.
How many nucleotides are required to code for these 20 amino acids? ___________________
19.
So, the language of DNA is a triplet code. How many unique triplets exist? ______________
20.
DNA is double-stranded, but for each protein, only one of these two strands is used to produce
an mRNA transcript. What is the coding strand called?
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21.
Here is a short DNA template. Below it, assemble the complementary mRNA strand.
3'A C
G A C C A G T A A A 5'
22. How many codons are there above? ________ Label one codon.
24. What was the first codonamino acid pair to be identified? __________________________
25. Of the 64 possible codons, how many code for amino acids? _________________________
26. What event is coded fro by UAA, UAG and UGA? ________________________________
27. What is the start codon? ____________________________________________________
28. Why is the genetic code said to be redundant but not ambiguous?
30. Now here is an important idea: DNA is DNA is DNA. By this we mean that the code is nearly
universal, and because of this, jellyfish genes can be inserted into pigs, or firefly genes can
make a tobacco plant glow. Enjoy a look at Figure 17.6 in your text . . . and no question to
answer here!
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32. You will recall from Chapter 16 that DNA polymerase III adds new nucleotides to the template
DNA strand to assemble each new strand of DNA. Both enzymes can assemble a new
polynucleotide only in the 5' direction. Which enzyme, DNA polymerase III or RNA
polymerase, does not require a primer to begin synthesis?
34. Figure 17.7 in your text will require a bit of study. Use it to label the following elements on the
figure below: promoter, RNA polymerase, transcription unit, DNA template, nontemplate
DNA,
and RNA transcript. Then, to the right of the figure, name the three stages of
transcription and briefly describe each stage.
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35. Lets now take a closer look at initiation. Read the paragraph titled RNA Polymerase Binding
and Initiation of Transcription carefully. List three important facts about the promoter
here.
(1)
(2)
(3)
36. Use Figure 17.8 in your text to label the following elements of the figure below: TATA box,
RNA polymerase II, transcription factors, template DNA strand, start point, 5' and 3', and
mRNA transcript. To the right of the figure, explain the three stages of initiation that are
shown.
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37. What is the TATA box? How do you think it got this name?
39. Now it is time to put all of the elements of transcription together. Write an essay below to
describe the process by which mRNA is formed. Use these terms correctly in your essay,
and
underline each one: TATA box, gene, terminator, promoter, elongation, 5 to 3',
termination,
initiation RNA, polymerase RNA nucleotides, template, start point,
termination signal, and transcription factors. This essay is typical of what you might be
asked to write on the AP Biology exam.
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Copyright 2010 Pearson Education, Inc.
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RNA processing occurs only in eukaryotic cells. The primary transcript is altered at both ends,
and sections in the middle are removed.
a. What happens at the 5' end?
b. What happens at the 3' end?
41.
What are three important functions of the 5' cap and poly-A tail?
42.
Distinguish between introns and exons. Perhaps it will help to remember this: Exons are
expressed.
43.
On the figure below, label: pre-mRNA, 5' cap, poly-A tail, introns, and exons.
44.
What are snRNPs? What two types of molecules make up a snurp? (We like the word snurp! It
reminds us of little cartoon characters that wore blue hoods and were called smurfs.
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45.
You will be introduced to a number of small RNAs in this course. What type is the RNA in a
snRNP?
46.
Snurps band together in little snurp groups to form spliceosomes. How do spliceosomes work?
47.
On the figure below, label the following: pre-mRNA, snRNPs, snRNA, protein, spliceosomes,
intron, and other proteins.
48.
Study the figure and text carefully to explain how the splice sites are recognized.
49.
What is a ribozyme?
50.
What commonly held idea was rendered obsolete by the discovery of ribozymes?
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51.
(2)
(3)
52.
You may need to read on in this section in order to answer this question as well as think back to
earlier information about mRNA. Come back to this question later if you wish. Three types
of RNA are needed for protein synthesis. Complete the chart below.
Type of RNA
mRNA
Description
Function
tRNA
rRNA
54.
What is an anticodon?
55.
Transfer RNA has two attachment sites. What binds at each site? Sketch tRNA, indicate the 2
attachment sites, and note where complementary base pairing and hydrogen bonding occur
to give tRNA its shape.
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56.
57.
Scientists expected to find one aminoacyl-tRNA synthetase per codon, but far fewer have been
discovered. How does wobble explain this?
58.
Use the figure below to explain the process of a specific amino acid being joined to a tRNA.
Also add these labels: aminoacyl-tRNA synthetase, ATP, amino acid, and tRNA.
59.
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60.
How does a prokaryotic ribosome differ from a eukaryotic ribosome? What is the medical
significance of this difference?
61.
On this figure, label the large subunit, small subunit, A, P, and E sites, mRNA binding site. To
the right of the figure, explain the functions of the A, P, and E sites.
62.
Much like transcription, we can divide translation into three stages. List them.
63.
Summarize the events of initiation. Include these components: small ribosomal subunit, large
ribosomal subunit, mRNA, initiator codon, tRNA, Met, initiation complex, P site, and GTP.
The
figure below may help you.
64.
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65.
Now, summarize the events of elongation. Include these components: mRNA, A site, tRNA,
codon, anticodon, ribozyme, P site, and E site. Again, the figure may help you.
66.
67.
What is a polyribosome?
68.
What are some of the things that will result in a final-form functional protein?
69.
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70.
Use the following figure to explain how proteins are targeted for the ER.
Concept 17.5 Point mutations can affect protein structure and function
71.
72.
73.
74.
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75.
76.
77.
78.
Concept 17.6 Although gene expression differs among the domains of life, the concept of a gene is
universal
79.
Describe two important ways in which bacterial and eukaryotic gene expression differ.
80.
What is a gene? It used to be simply stated that one gene codes for one polypeptide. That
definition has now been modified. Write below the broader molecular definition in use
today.
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81.
Finally, use this summary figure to put together all that you have learned in this chapter.
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