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    PhysioEx Exercise 1 Act 1

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    Juan Alemán
    1. Juan Alemán completed an activity on cell transport mechanisms involving dialysis experiments using different membranes and solutes. 2. The experiments tested diffusion of sodium, chloride, urea, albumin, and glucose through membranes with pore sizes of 20MWCO, 50MWCO, 100MWCO, and 200MWCO. 3. The results showed that sodium diffused through the 50MWCO and 100MWCO membranes, but larger molecules like urea, albumin, and glucose did not diffuse through the smaller 20MWCO or 50MWCO membranes as their sizes exceeded the pore thresholds.

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    PhysioEx Exercise 1 Act 1

    Uploaded by

    Juan Alemán
    754 views5 pages
    1. Juan Alemán completed an activity on cell transport mechanisms involving dialysis experiments using different membranes and solutes. 2. The experiments tested diffusion of sodium, chloride, urea, albumin, and glucose through membranes with pore sizes of 20MWCO, 50MWCO, 100MWCO, and 200MWCO. 3. The results showed that sodium diffused through the 50MWCO and 100MWCO membranes, but larger molecules like urea, albumin, and glucose did not diffuse through the smaller 20MWCO or 50MWCO membranes as their sizes exceeded the pore thresholds.

    Original Description:

    Answers of PEx 9.1

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    1. Juan Alemán completed an activity on cell transport mechanisms involving dialysis experiments using different membranes and solutes. 2. The experiments tested diffusion of sodium, chloride, urea, albumin, and glucose through membranes with pore sizes of 20MWCO, 50MWCO, 100MWCO, and 200MWCO. 3. The results showed that sodium diffused through the 50MWCO and 100MWCO membranes, but larger molecules like urea, albumin, and glucose did not diffuse through the smaller 20MWCO or 50MWCO membranes as their sizes exceeded the pore thresholds.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    754 views5 pages

    PhysioEx Exercise 1 Act 1

    Uploaded by

    Juan Alemán
    1. Juan Alemán completed an activity on cell transport mechanisms involving dialysis experiments using different membranes and solutes. 2. The experiments tested diffusion of sodium, chloride, urea, albumin, and glucose through membranes with pore sizes of 20MWCO, 50MWCO, 100MWCO, and 200MWCO. 3. The results showed that sodium diffused through the 50MWCO and 100MWCO membranes, but larger molecules like urea, albumin, and glucose did not diffuse through the smaller 20MWCO or 50MWCO membranes as their sizes exceeded the pore thresholds.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 5

    Name: Juan Alemn

    Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report
    Pre-lab Quiz Results
    You scored 100% by answering 4 out of 4 questions correctly.
    1. The driving force for diffusion is
    You correctly answered: b. the kinetic energy of the molecules in motion.
    2. In diffusion, molecules move
    You correctly answered: a. from high concentration to low concentration.
    3. Which of the following dialysis membranes has the largest pore size?
    You correctly answered: d. 200 MWCO
    4. Avogadro's number is a constant for the number of
    You correctly answered: b. molecules.

    05/23/16

    page 1

    Experiment Results
    Predict Question:
    Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
    Your answer : c. No, not at all.
    Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average
    molecular weight of a single amino acid is 135 g/mole.
    Which of the following will be able to diffuse through the 200 MWCO membrane?
    Your answer : b. both glucose and albumin
    Stop & Think Questions:
    The reason sodium chloride didn't diffuse left to right is that
    You correctly answered: c. the membrane pore size was too small.
    Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino
    acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because
    You correctly answered: d. glucose and albumin are both too large to pass.
    The rate of diffusion for urea
    Your answer : c. is faster than that for sodium because urea is a smaller molecule.
    Correct answer: b. is slower than that for sodium because urea is a larger molecule.
    Experiment Data:
    Solute

    MWCO

    Left Solute Concentration

    Right Solute Concentration

    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ Cl-

    20
    20
    20
    20
    20
    20
    20
    20
    50
    50
    50
    50
    50
    50
    50
    50
    100
    100
    100
    100
    100

    9.00
    0.00
    0.00
    0.00
    0.00
    9.00
    0.00
    0.00
    9.00
    0.00
    0.00
    0.00
    18.00
    0.00
    0.00
    0.00
    9.00
    0.00
    0.00
    0.00
    0.00

    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00

    05/23/16

    page 2

    Average Diffusion
    Rate
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0150
    0.0000
    0.0000
    0.0000
    0.0273
    0.0000
    0.0000
    0.0000
    0.0150
    0.0000
    0.0000
    0.0000
    0.0000

    Solute

    MWCO

    Left Solute Concentration

    Right Solute Concentration

    Urea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose

    100
    100
    100
    200
    200
    200
    200
    200
    200
    200
    200

    9.00
    0.00
    0.00
    0.00
    0.00
    0.00
    6.00
    0.00
    0.00
    9.00
    0.00

    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00

    05/23/16

    page 3

    Average Diffusion
    Rate
    0.0094
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0029
    0.0000
    0.0000
    0.0000
    0.0000

    Post-lab Quiz Results


    You scored 75% by answering 3 out of 4 questions correctly.
    1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
    You correctly answered: b. increase the rate of diffusion.
    2. Describe the difference between the rate of diffusion seen for sodium and urea.
    Your answer: d. Urea diffused more slowly because it is smaller than sodium.
    Correct answer: c. Urea diffused more slowly because it is larger than sodium.
    3. Which of the following solutes did not pass through any of the membranes?
    You correctly answered: b. albumin
    4. When diffusion stops, we say the solution has reached
    You correctly answered: a. equilibrium.

    05/23/16

    page 4

    Review Sheet Results


    1. Describe two variables that affect the rate of diffusion.
    Your answer:
    Concentration.
    MWCO.
    2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare
    with your prediction?
    Your answer:
    Because the size of molecule.
    3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the
    results compare with your prediction?
    Your answer:
    Ok.
    4. Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.
    Your answer:
    Sodium Chloride.
    Urea.
    Glucose.
    Albumin.

    05/23/16

    page 5

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