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    Physioex 1.1

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    Tj Mordecki
    1. Thomas Mordecki completed an activity on cell transport mechanisms involving dialysis experiments using membranes of varying molecular weight cut-off (MWCO). 2. He correctly answered pre-lab and post-lab quiz questions about diffusion rates and molecular properties. 3. The experiment results showed that sodium diffused through all membranes as expected, while larger molecules like urea and glucose only diffused through higher MWCO membranes, and albumin did not diffuse at all, matching Thomas's predictions.

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    Physioex 1.1

    Uploaded by

    Tj Mordecki
    214 views5 pages
    1. Thomas Mordecki completed an activity on cell transport mechanisms involving dialysis experiments using membranes of varying molecular weight cut-off (MWCO). 2. He correctly answered pre-lab and post-lab quiz questions about diffusion rates and molecular properties. 3. The experiment results showed that sodium diffused through all membranes as expected, while larger molecules like urea and glucose only diffused through higher MWCO membranes, and albumin did not diffuse at all, matching Thomas's predictions.

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    Physioex ex1 activity1

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    1. Thomas Mordecki completed an activity on cell transport mechanisms involving dialysis experiments using membranes of varying molecular weight cut-off (MWCO). 2. He correctly answered pre-lab and post-lab quiz questions about diffusion rates and molecular properties. 3. The experiment results showed that sodium diffused through all membranes as expected, while larger molecules like urea and glucose only diffused through higher MWCO membranes, and albumin did not diffuse at all, matching Thomas's predictions.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    214 views5 pages

    Physioex 1.1

    Uploaded by

    Tj Mordecki
    1. Thomas Mordecki completed an activity on cell transport mechanisms involving dialysis experiments using membranes of varying molecular weight cut-off (MWCO). 2. He correctly answered pre-lab and post-lab quiz questions about diffusion rates and molecular properties. 3. The experiment results showed that sodium diffused through all membranes as expected, while larger molecules like urea and glucose only diffused through higher MWCO membranes, and albumin did not diffuse at all, matching Thomas's predictions.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 5

    Name: Thomas Mordecki

    Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report
    Pre-lab Quiz Results
    You scored 100% by answering 4 out of 4 questions correctly.
    1. The driving force for diffusion is
    You correctly answered: b. the kinetic energy of the molecules in motion.
    2. In diffusion, molecules move
    You correctly answered: a. from high concentration to low concentration.
    3. Which of the following dialysis membranes has the largest pore size?
    You correctly answered: d. 200 MWCO
    4. Avogadro's number is a constant for the number of
    You correctly answered: b. molecules.

    05/24/15

    page 1

    Experiment Results
    Predict Question:
    Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
    Your answer : c. No, not at all.
    Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average
    molecular weight of a single amino acid is 135 g/mole.
    Which of the following will be able to diffuse through the 200 MWCO membrane?
    Your answer : c. glucose only
    Stop & Think Questions:
    The reason sodium chloride didn't diffuse left to right is that
    You correctly answered: c. the membrane pore size was too small.
    Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino
    acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because
    You correctly answered: d. glucose and albumin are both too large to pass.
    The rate of diffusion for urea
    You correctly answered: b. is slower than that for sodium because urea is a larger molecule.
    Experiment Data:
    Solute

    MWCO

    Left Solute Concentration

    Right Solute Concentration

    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea

    20
    20
    20
    20
    20
    20
    20
    20
    50
    50
    50
    50
    50
    50
    50
    50
    100
    100
    100
    100
    100
    100

    9.00
    0.00
    0.00
    0.00
    0.00
    9.00
    0.00
    0.00
    9.00
    0.00
    0.00
    0.00
    18.00
    0.00
    0.00
    0.00
    9.00
    0.00
    0.00
    0.00
    0.00
    9.00

    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00

    05/24/15

    page 2

    Average Diffusion
    Rate
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0150
    0.0000
    0.0000
    0.0000
    0.0273
    0.0000
    0.0000
    0.0000
    0.0150
    0.0000
    0.0000
    0.0000
    0.0000
    0.0094

    Solute

    MWCO

    Left Solute Concentration

    Right Solute Concentration

    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose
    Na+ ClUrea
    Albumin
    Glucose

    100
    100
    200
    200
    200
    200
    200
    200
    200
    200

    0.00
    0.00
    0.00
    0.00
    0.00
    9.00
    0.00
    0.00
    9.00
    0.00

    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00
    0.00

    05/24/15

    page 3

    Average Diffusion
    Rate
    0.0000
    0.0000
    0.0000
    0.0000
    0.0000
    0.0042
    0.0000
    0.0000
    0.0000
    0.0000

    Post-lab Quiz Results


    You scored 100% by answering 4 out of 4 questions correctly.
    1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
    You correctly answered: b. increase the rate of diffusion.
    2. Describe the difference between the rate of diffusion seen for sodium and urea.
    You correctly answered: c. Urea diffused more slowly because it is larger than sodium.
    3. Which of the following solutes did not pass through any of the membranes?
    You correctly answered: b. albumin
    4. When diffusion stops, we say the solution has reached
    You correctly answered: a. equilibrium.

    05/24/15

    page 4

    Review Sheet Results


    1. Describe two variables that affect the rate of diffusion.
    Your answer:
    The molecular weight and temperature can affect the rate of diffusion. Larger molecules diffuse slower than smaller
    molecules. As temperature rises, the molecules move faster.
    2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare
    with your prediction?
    Your answer:
    I thought Urea wouldn't be able to diffuse through because it is too large and would not fit.
    3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the
    results compare with your prediction?
    Your answer:
    I thought glucose would diffuse because it is so much smaller. Albumin is a very large molecule and, because of its size, it
    would not be able to diffuse through a 20 MWCO membrane.
    4. Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.
    Your answer:
    Sodium, Urea, Glucose, Albumin

    05/24/15

    page 5

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