Compatibility Testing
Compatibility Testing
Compatibility Testing
( PRETRANSFUSION TESTING)
2. cut the end of the segment next to the cell pack & use an
applicator stick to remove the cells or express a drop by
squeezing the tubing.
5. Crossmatching
Abbreviated Crossmatch:
an immediate spin, saline, major cross match used
to coupled with type and screen is used to
determine ABO compatibility in patient with
no demonstrable clinically significant antibodies &
(-) history of antibody formation
Crossmatching
2. Incubation at 370C ( High Protein Phase)
1. decreased workload
2. reduced volume of patients blood samples
3. reduced exposure of personnel to blood specimens
4. elimination of false reactions associated with the IS
cross match
Gel Technology
Newer Routine Non-test tube Method for
Compatibility Testing:
Innovative technique, invented buy Dr. Yves Lapierre
of France
To minimize problems associated with conventional
techniques
Now used worldwide
utilizes a small plastic card that contains six
microtubes, each filled with a gel for a specific test
3 kinds of gel available:
a) specific gel( ABO, Rh type & other phenotyping)
b) buffered gel for reverse typing
C) antiglobulin gel ( DAT/IAT)
Principle of Gel Technology
1. both the gel & the diluent used have low ionic
strength characteristics
labor intensive
skilled reading is required
Instability of completed test
Poor wash phase; false weak/negative
Inappropriate handling of completed test; results to
downgrade/negative
addition of sensitized control cells to check negative
results
Advantages of Gel Technology
Design of a crossmatch:
A. Provide conditions suitable for optimum reactivity
of all clinically significant Abs
a.1 temperature of reactivity
a.2 medium of reaction
B. Specimen used:
b.1 patients serum- not more than 48 hours old
b.2 not inactivated
b.3 not plasma
Defining the Problem