M318E 03.12.CF.

1(1/2)

Microscope
ECLIPSE 80i
Instructions
<Microscopy>
Introduction
Thank you for purchasing this Nikon product.
This instruction manual, which describes basic microscope operations, is intended for users of the Nikon ECLIPSE 80i
microscope.
To ensure correct use, please read this manual carefully before operating the product.

This manual may not be reproduced or transmitted in whole or in part without Nikon's express consent.

The contents of this manual are subject to change without notice.

Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies
may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon
representative.

Some of the products described in this manual may not be included in the set you have purchased.

Make sure you have read the manuals for any other products attached to or to be used with this
microscope (super high-pressure mercury lamp power supply, high-intensity light source, etc.).

Warning/Caution symbols used in this manual

Although Nikon products are designed to provide the utmost safety, ignoring safety precautions or improper use may
result in personal injury or property damage, as well as voiding the terms of the warranty. To ensure safe use, please
read the instruction manual carefully and thoroughly before trying to operate the instrument. Do not discard this
manual. Store in a convenient location near the product for ready reference.
In this manual, safety precautions are indicated by the following symbols. For safe, correct use of the microscope,
always follow the instructions indicated by these symbols.

Symbol Meaning

Disregarding instructions indicated by this symbol may result in death or serious

WARNING injury.

Disregarding instructions indicated by this symbol may result in injury or property

CAUTION damage.

1
 Introduction

Meaning of symbols used on the product

When appearing on the product, the symbols below indicate the need for caution at all times during use.
Consult the instruction manual and read the relevant instructions before attempting to use or adjust any part to which
the symbol has been affixed.

Caution! Biohazard

This symbol found on the stage indicates the following:0

WARNING: Contact between sample and microscope may result in biohazard risks.

To avoid biohazard contamination, avoid touching the contaminated portion with bare hands.

Decontaminate the contaminated part according to the standard procedure specified for your
laboratory.

Caution for heat

This symbol found on the lamphouse of the ECLIPSE 80i indicates the following:

The lamp and surrounding areas (including the lamphouse) become very hot during and
immediately after a period of illumination.

Risk of burns. Do not touch the lamp or surrounding areas during or immediately after a
period of illumination.

Make sure the lamp and surrounding areas have cooled sufficiently before attempting to
replace the lamp

2
Safety Precautions

Please follow the safety precautions given below.

WARNING

1. Intended use of the product

This product is intended primarily for microscopic observations of cells and tissue set on glass sides
using diascopic (transmitted) and episcopic illumination.
It is intended for use in experimentation and observation of cells and tissue in the fields of pathology
and cytology in hospital and other laboratory settings.

2. Do not disassemble.

Disassembly may result in malfunctions and/or electrical shock and will void the terms of the warranty.
Never attempt to disassemble any part other than the parts described in this manual. If you experience
problems with the product, contact your nearest Nikon representative.

3. Read the instruction manuals carefully.

To ensure safety, carefully read this manual and the manual provided with any other equipment used
with this product. Observe all warnings and cautions given at the beginning of each manual.

When the digital imaging head or the D-FL Epi-illumination attachment is attached to the
microscope
The mercury lamp (or xenon lamp) for Epi-fl microscopy requires special care during handling. Make
sure you have read the instruction manual for the light source (super high-pressure mercury lamp
power supply or high-intensity source).

4. Power cord for microscope and the power cord for AC adapter

Use one of the power cords specified. Using the wrong power cord may result in fire or other hazards.
The product is classified as subject to Class I protection against electrical shock. Make sure it is
connected to an appropriate ground terminal.
Refer to Chapter 8 for the power cords specified.
To prevent electric shock, always turn off the main power switch (press it to the "{" position) of the
product before attaching or detaching the power cord.

5. Inspect the AC adapter (when using the D-CB C-BOX).

The D-CB C-BOX is powered by an AC adapter. Use only the specified adapter model meeting the
requirements. Using any other type of adapter may result in malfunction, overheating, and/or fire.
Refer to Chapter 8 for the adapter specified.
To prevent malfunctions and/or fire, use the AC adapter in a well-ventilated location. To ensure
proper heat radiation and to prevent overheating, never cover or place any object on the adapter.

To prevent malfunctions, always turn off the power switch (the switch is in the out position) of the
C-BOX before attaching the AC adapter.

3
 Safety Precautions

WARNING
6. Heat from the light source
The lamp and surrounding areas (including the lamphouse) will become very hot during and
immediately after a period of illumination.

Risk of burns. Never touch the lamp or surrounding areas during or immediately after a period of
illumination.

Make sure the lamp and surrounding areas have cooled sufficiently before attempting to replace the
lamp

To avoid risk of fire, do not place fabric, paper or highly flammable volatile materials such as
gasoline, petroleum benzine, paint thinner or alcohol near the lamphouse while the lamp is lit or
during a period of around thirty minutes after the lamp has been turned off.

7. Mercury lamps and xenon lamps (when the digital imaging head or the D-FL Epi-illumination
attachment is attached)
The mercury lamp (or xenon lamp) for the digital imaging head or the D-FL Epi-illumination
attachment requires special care during handling. For safe and correct use of this system, carefully
read the warnings below. Keep in mind all potential hazards. Additionally, carefully read the manual for
the super high-pressure mercury lamp power supply (or high-intensity light source) and the manual (if
provided) from the lamp manufacturer, then follow the instructions given therein.

Hazards of Mercury Lamps and Xenon Lamps

1) When lit, mercury (and xenon) lamps radiate ultraviolet light that can damage your eyes and skin.
Direct viewing of the light may result in blindness.
2) The lamps contain sealed gas under very high pressure, pressure that increases when the lamp is on.
If the lamp is scratched, fouled, subjected to high external pressure or physical impact, or used
beyond its service life, the sealed gas may escape or the lamp may burst, resulting in gas inhalation,
injury from glass, or other injury.
3) When the lamp is lit, the lamp and surroundings will become extremely hot. Touching the lamp with
bare hands may result in burns; flammable materials placed near the lamp may ignite.
4) Using the wrong lamp type may result in accidents, including bursting of the lamp.

Safety is a top design priority for Nikon products. The preceding hazards should pose no danger as
long as the user observes all of the warnings and cautions given in the manuals, and uses the system
only for its intended purpose.
However, failure to heed the warnings and cautions given in the manuals, subjecting the system to
shock or impact, or attempting to disassemble the system may result in accidents and injury. Make
sure you are familiar with and adhere to all warnings and cautions.

8. Always turn off the lamp when changing filter cubes (when the digital imaging head or the D-FL
Epi-illumination attachment is attached to the microscope).
When changing filter cubes, always turn off the digital imaging head or the D-FL Epi-illumination
attachment. Leaving the lamp on may result in ultraviolet exposure.

9. Leave the D/UV slider in the optical path (when using the digital imaging head or the D-FL
Epi-illumination attachment).
Always leave the D/UV slider (which is one of the D-ES EPI ND slider types) in the optical path when
performing Epi-bright-field microscopy, Epi-dark-field microscopy, or Epi-DIC microscopy with the
mercury lamphouse attached. If the slider is moved out of the path, ultraviolet light will be radiated
and damage your eyes.

4
 Safety Precautions

WARNING
10. Hazardous Sample
This microscope is intended primarily for microscopic observations of cells and tissue set on glass
sides.
Check to determine whether a sample is hazardous before handling.
Handle hazardous samples according to the standard procedure specified by your laboratory.
If the sample is potentially infectious, wear rubber gloves and avoid touching samples. If contact
occurs between a sample and the microscope, decontaminate the contaminated portion according to
the standard procedure specified for your laboratory.

CAUTION
1. Turn off power during assembly, connection/disconnection of cords, lamp replacement, and
maintenance.

To prevent electric shock and/or malfunctions, always turn off the power switch(es) of the product
(press it (them) to the "!" position) and unplug the power cord from the wall outlet before assembly,
connecting or disconnecting of cords, lamp replacement, and cleaning of the microscope and the
objective.

2. Lamp replacement precautions

To avoid burns, wait at least 30 minutes after the lamp is turned off to give it sufficient time to cool.
To avoid electric shock or malfunctions, never attempt to replace the lamp without first turning off the
power switches for the microscope and the peripheral devices (press them to the "!" position) and
unplugging the power cord from the wall outlet.
Make sure the lamphouse cover is securely fitted to the lamphouse after lamp replacement. Never turn
on the lamp while the lamphouse cover is open. Do not break up used lamps; instead, dispose of them
as special industrial waste or as specified by local regulations.

3. Use the specified lamp and lamphouse.

Always use the specified lamp and lamphouse. Using an unspecified lamp may cause malfunction or fire.
Refer to Chapter 8 for the specified lamp and lamphouse.

4. Avoid contact with water.

Never allow water to come into contact with the product, and keep the product away from liquids.
Splashing water onto the product may cause a short, resulting in malfunction or abnormal heating. If
water is splashed onto the product, immediately turn off the power switch (press it to the "!" position)
and remove the power cord from the receptacle. Then wipe off moisture with a dry cloth or something
similar. If water enters the product, do not use; in this case, contact your distributor.

5. Do not place any object on top of the product.

Do not place any object on top of the product or cover it with a cloth or the like. The system
temperature will rise, resulting in malfunctions.

5
 Safety Precautions

CAUTION
6. Cautions on assembling and installing the microscope

Take care to avoid pinching your fingers or hands during microscope assembly.

Scratches or fouling such as fingerprints on optical components (such as lens and filters) will
degrade microscope images. Be careful to avoid scratches or direct contact with the lens and filters.

The main unit weighs about 12 kg. Grasp the main unit by the recesses at the front of the arm and
at the bottom of the main unit.

Remove all attachments (if attached) from the microscope before carrying the microscope.

Do not install the product in a locker or cabinet.

7. Remove any covers from the product before switching on.

Do not use the product while covered with a cloth, etc., as this will give rise to abnormal heat, which
could cause a fire.

8. Caution concerning long, sustained observations

To relieve fatigue resulting from long observation sessions, limit continuous observations to one hour.
Take at least 10- to 15-minute breaks between observation sessions. Adjust the layout of other
equipment (such as the display and the mouse) and match to the position of the product and the height
of your chair.

9. Disposal of the product

To avoid biohazard risks, dispose of the product as contaminated equipment according to the standard
procedure specified for your laboratory.

6
 Notes on handling the product

Notes on handling the product

1. Handle the product gently.

This product is a precision optical instrument and requires gentle handling. Avoid subjecting it to sudden
impact and shocks.
Even relatively minor impacts are capable of affecting the precision of the object.

2. Weak electromagnetic waves

The product emits weak electromagnetic waves. Do not place precision electronic devices near the
product to avoid degrading their performance. If TV or radio reception is affected, move the TV or radio
farther away from the product.

3. Scratches, dirt, and foreign particles on the lens

Scratches or fouling such as fingerprints on optical components (such as lens and filters) will degrade
microscope images. If these parts become dirty, clean them as described in chapter "7. Care and
maintenance" at the end of this manual.

4. Dirt on the lamps

Never touch the lamp with bare hands. Dirt or fingerprints on the lamp will result in uneven illumination
and reduce the service life of the lamp. Always wear gloves when handling lamps.

5. Installation location

This product is a precision instrument. Use or storage in inappropriate environments may result in
malfunctions or poor performance. Consider the following factors when selecting an installation location:

Select a vibration-free location. Install the product on a level surface.

Install the product at least 10 cm away from walls.

Choose a location less exposed to hazards in the event of collisions, earthquakes, or other potential
disasters. To keep the product from falling, use strong rope or other means if necessary to secure it
to the working desk or to another heavy, stable item. To fix the microscope, there are two M6 screw
holes (one at the right and the other at the left) behind the recess at the base of the main body
(back side).

Avoid locations exposed to direct sunlight, locations immediately under room lights, and other bright
locations.

Avoid locations with excessive dust.

Do not install the product near any liquid.

Make sure the ambient temperature is 0 to 40C and humidity is 85% or less. Installing the 80i in hot,
humid locations may result in mildew formation or condensation, impairing performance or
generating malfunctions.

Do not install the product in a locker or cabinet.

Select a layout that allows easy removal of the power cord from the product's AC inlet in the event of
an emergency.

Room lights just above the microscope may reduce visibility in the objective as extraneous light. If
possible, switch off room lights directly above the microscope when making observations.

7
 Notes on handling the product

6. Focusing knobs

Never turn the focus knobs on the left and right sides of the microscope in opposite directions at the
same time. Doing so may damage the microscope.

Turning the coarse focus knob past its farthest point will damage the microscope. Never use undue
force when turning the knob.

7. Protect the ports from dust and extraneous light (when the trinocular eyepiece tube, the digital
imaging head, or the C-TE ergonomic binocular tube is attached).

To keep out extraneous light and dust, always attach the supplied cap to any port not currently in use.

8. Handling of filters (when the digital imaging head or the D-FL Epi-illumination attachment is
attached to the microscope)

Interference filters (especially excitation filters, which are exposed to strong light) degrade over time.
Replace them after the appropriate hours.

Filter characteristics may alter if the filter is exposed to high humidity. To prevent changes in or
degradation of filter characteristics, avoid using or storing the filters under conditions of high humidity
or high temperature. Avoid subjecting filters to rapid temperature changes. When a filter is not in use,
store in a desiccator or hermetically sealed container with a drying agent.

The filters in the nine types of filter cubes listed below offer sharp, high-resolution waveform
characteristics superior to normal filters. However, due to their sophisticated coatings, they must be
handled with special care. In particular, take care to avoid abrasion from cleaning. (Follow the
procedure described in section "1. Filter and lens cleaning" of chapter "7. Cleaning and Maintenance.")
Single-band filter cubes: DAPI, FITC, TxRed, GFP
Multi-band filter cubes: F-R, F-T, D-F, D-F-R, D-F-T

9. Handling of the D-FLD dark-field illumination cube (when using the digital imaging head or the D-FL
Epi-illumination attachment)

There are two wings in front of the D-FL Epi-illumination attachment, these prevent light leakage. Do not
apply any pressure onto the wings, as malfunction may result.

10. Unpacking and unclamping

1) Check the contents to ensure that the package contains the following:

ECLIPSE 80i x1

Tools: Hex wrench x2

Allen key x1

Accessories: Shield cover x1

Dust-proof cover x1

2) Before connecting the power cord, remove the cushioning material from under the sub-stage by turning
the focusing handle and raising the sub-stage.

3) Do not discard the packing case, as it may be needed for future transportation.

8
 Abbreviations Used in The Manual

Abbreviations Used in The Manual

The product names and abbreviations used in this manual are given below.

The manual uses the following abbreviations:

Name of device Abbreviation

Microscope ECLIPSE 80i 80i

C-ER Eye Level Riser Eye Level Riser

C-TE Ergonomic Binocular Tube Ergonomic Binocular Tube

C-TEP DSC Port for Ergonomic Binocular Tube DSC Port

D-FL Epi-illumination Attachment Epi-illumination Attachment

D-DH Digital Imaging Head M DIH-M

D-CB C-Box C-Box

D-LH Precentered Lamphouse Lamphouse

D-NID6 Intelligent Sextuple DIC Nosepiece Intelligent Nosepiece

C-HS Hand Switch Hand Switch

DS Camera Head DS-5M Camera Head

DS Camera Control Unit DS-L1 DS-L1

DS Camera Cable Camera Cable

Super High-pressure Mercury Lamp Power Supply Unit Mercury Lamp Power Supply Unit

Super High-pressure Mercury Lamphouse Mercury Lamphouse

9
 How to use this instruction manual

How to use this instruction manual

This instruction manual is composed of two parts, as below:

Manual 1 "Microscopy" describes basic microscope operations that you must follow. Please read
this manual carefully before operating the product.

Manual 2 "Reference" describes the operations of each attachment. Please read an appropriate
section as necessary.

Title Importance Content

Manual 1 Microscopy Must be read Safety precautions

Microscopy, Assembly, Troubleshooting,

Cleaning and maintenance, Specifications

Manual 2 Reference As necessary Detailed operations of attachments

10
 Using Microscopy Manual

Using Microscopy Manual

The part names and microscopy procedures are described in three separate sections,
corresponding to specific configurations of 80i and accessory devices. Confirm the combination
of the 80i and accessory devices you are using, then refer to the appropriate section.

Combination Description

Bright-field set See Chapter 1.

(Eyepiece tube mounted (For operating

directly on 80i) procedures and
detailed information on
(Camera and monitor are
individual parts, refer
optional.)
to the separate
Reference manual.)

80i + Epi-illumination See Chapter 2.

attachment
(For operating
(Camera and monitor are procedures and
optional.) detailed information on
individual parts, refer
to the Reference
manual.)

80i + DIH-M See Chapter 3.

(Camera and monitor are (For operating

optional.) procedures and
detailed information on
individual parts, refer
to the Reference
manual.)

11
 Contents

Contents

Introduction ....................................................................................................... 1
Warning/Caution symbols used in this manual .................................................................. 2
Meaning of symbols used on the product ......................................................................... 2
Safety Precautions .............................................................................................. 3
WARNING.................................................................................................... 3
CAUTION..................................................................................................... 5
Notes on handling the product .............................................................................. 7
Abbreviations Used in The Manual ......................................................................... 9
How to use this instruction manual .......................................................................10
Using Microscopy Manual ....................................................................................11
Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set) .........................14
1.1 Names of Main Components ............................................................................... 14
1.2 Names of Parts Used to Make Adjustments........................................................... 15
1.2.1 Right view ................................................................................................... 15
1.2.2 Left view ..................................................................................................... 16
1.2.3 Rear view.................................................................................................... 16
1.2.4 Ergonomic binocular tube with camera attached............................................... 17
1.3 Bright-Field Microscopy ...................................................................................... 18
1.4 Photomicroscopy............................................................................................... 22
Chapter 2 Part Names and Microscopy Procedures
(With Epi-illumination Attachment Mounted) ..........................................24
2.1 Names of Main Components ............................................................................... 24
2.2 Names of Parts Used to Make Adjustments........................................................... 25
2.2.1 Right view ................................................................................................... 25
2.2.2 Left view ..................................................................................................... 26
2.2.3 Rear view (without accessory device) ............................................................. 26
2.3 Bright-Field Microscopy ...................................................................................... 27
2.4 Epi-fluorescence Microscopy ............................................................................... 31
2.5 Photomicroscopy............................................................................................... 35
Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)) ..................37
3.1 Names of Main Components ............................................................................... 37
3.2 Names of Parts Used to Make Adjustments........................................................... 38
3.2.1 Right view ................................................................................................... 38
3.2.2 Left view ..................................................................................................... 39
3.2.3 Rear view (without accessory device) ............................................................. 40
3.3 Bright-Field Microscopy ...................................................................................... 41
3.4 Epi-fluorescence Microscopy ............................................................................... 45
3.5 Photomicroscopy............................................................................................... 49
Chapter 4 Assembly ...........................................................................................51
Chapter 5 Replacing Consumables ........................................................................63
5.1 Replacing the lamp............................................................................................ 63

12
 Contents

Chapter 6 Troubleshooting...................................................................................64
6.1 Optical............................................................................................................. 64
6.2 Electrical .......................................................................................................... 65
Chapter 7 Cleaning and Maintenance ....................................................................66
7.1 Lens cleaning ................................................................................................... 66
7.2 Cleaning the product ......................................................................................... 66
7.3 Disinfecting the product ..................................................................................... 66
7.4 Storage............................................................................................................ 67
7.5 Periodic Inspections (fee charged)....................................................................... 67
Chapter 8 Specifications......................................................................................68
8.1 Specifications ................................................................................................... 68

13
 1 Part Names and Microscopy Procedures
(Bright-Field Set)

1.1 Names of Main Components

Eyepiece Eyepiece tube

Camera head
(Required for image capture)
(One example shown in the photo)

DSC port
(Required for image capture)
Revolving
nosepiece

Objective
Lamphouse
Specimen holder

Stage

Condenser

Microscope (main body)

Camera control unit

(Required for image capture)
(One example shown in the photo)

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.2 Names of Parts Used to Make Adjustments

1
1.2 Names of Parts Used to Make Adjustments

1.2.1 Right view

Diopter
adjustment
ring

Optical path
switching lever

Aperture diaphragm
lever

Y stage knob

X stage knob

(Provided on the left side

in certain products)

Filter IN/OUT
switches

Field diaphragm knob Fine focus knob

Coarse focus
upper-limit clamp

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.2 Names of Parts Used to Make Adjustments

1.2.2 Left view

Condenser focus knob

Coarse focus
(Provided on the
torque adjustment
right side in knob
certain products)
Coarse focus knob

Condenser
Fine focus knob centering
screw

Brightness Preset switch Power ON

control knob indicator

1.2.3 Rear view

Tool storage
compartment

Power switch

AC power inlet

Input voltage
indication

Lamphouse

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.2 Names of Parts Used to Make Adjustments

1
1.2.4 Ergonomic binocular tube with camera attached

Camera cable connector

Camera head

C mount

Camera fine focus

adjustment screw
Camera centering screws

17
 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.3 Bright-Field Microscopy

1.3 Bright-Field Microscopy

1 Turn on power.

Press the power

switch to the "|"
position.

2 Press the preset switch.

Press the preset switch.

3 Move the ND8, ND32, and NCB11

filters into the optical path.

NCB11
ND32
ND8

The full optical

4 Push in the optical path

switching lever to direct the full
path directed
toward the
binocular
Push in the optical
path-switching lever.
section
optical path toward the
binocular section.

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.3 Bright-Field Microscopy

1
5 Raise the condenser to the
uppermost position.

Raise the condenser using the

condenser focus knob.

6 Fully open the field diaphragm

and aperture diaphragm.
(When using the 1-100x condenser, move the top
lens into the optical path.)

Fully open the Fully open the field

aperture diaphragm diaphragm using the
using the aperture field diaphragm knob.
diaphragm lever.

7 Set the 10x objective into the

optical path.

Select
the 10x
objective.

8 Set a specimen and move the

portion to be viewed into the
Set a specimen and secure in
place using the specimen holder.

optical path.

Move the portion to be viewed into the

optical path using the XY stage knobs.

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.3 Bright-Field Microscopy

9 Focus on the specimen.

Focus on the specimen using

the coarse and fine focus knobs.

10 Adjust the diopter and the

interpupillary distance.
Refer to "8. Diopter Adjustment" and "9.
Interpupillary Distance Adjustment" in the separate
Reference manual.

11 Focus and center the condenser.

Refer to "11. Adjustment of Condenser Position" in
the separate Reference manual.

Focus the condenser Center the condenser

using the condenser using the condenser
focus knob. centering screws.

Field diaphragm Field diaphragm

Eyepiece field Eyepiece field

of view of view

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.3 Bright-Field Microscopy

1
12 Switch to the desired objective
and view the specimen.
Adjust the field diaphragm and
aperture diaphragm each time
you change objectives.

Field diaphragm:
Select the
Set slightly narrower than the margins of desired objective.
the field of view.

Aperture diaphragm:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective

Aperture diaphragm Field diaphragm knob

knob

13 Turn off power after completing

observations.

Press the power

switch to the ""
position.

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 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.4 Photomicroscopy

1.4 Photomicroscopy

For detailed explanations of the camera, photomicroscopic software, and PC, refer to the
operating manuals provided with the respective products. The following instructions assume a
DS-5M digital camera and DS-L1 camera control unit.

1 Adjust the microscope for proper image observation.

See the directions given earlier in "1. Bright-Field Microscopy."

2 Adjust the camera head mounting position until the image is

displayed properly.
(1) Adjustment based on stage motion direction
Loosen the camera centering screws on the C
mount and adjust the camera position so that
moving the stage forward-backward or
left-right moves the image on the monitor in
the same direction. After making the (B)
appropriate adjustments, tighten the screws
(A)
firmly.

(2) Focus adjustment

If the image viewed through the eyepiece
appears to be in focus but the image on the
monitor is out of focus, turn the camera fine
Adjusting (A) and (B)
focus adjustment screw on the C mount until
the image on the monitor is in focus. (Use a
low-magnification lens to make this
adjustment.)
Note that such out of focus situations can also
indicate incorrect diopter adjustment. Make
sure you have made diopter adjustments
before adjusting camera focus. (Refer to the
separate Reference manual.)

Camera centering screws Camera fine focus

3
adjustment screw
Make camera settings.
For a detailed discussion of this topic, refer to the operating manual provided with the camera.
When using the DS-L1, you must choose and enter at least the following information:

Folder for data storage.

Name of file to be saved. (You can select "Auto.")

File format and file size.

Date and destination of data

22
 Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)

1.4 Photomicroscopy

1
4 Select the camera scene mode suitable for the microscopy
method.

5 Set the camera white balance.

To adjust white balance, press the WB button while capturing an image of a clear section of a
specimen slide. (For fluorescent photomicrography, adjust white balance under normal lighting
conditions before shooting.)

6 Capture and save images.

Focus on the specimen.
Refocus.
Adjust image brightness using the camera exposure compensation function.
Check the image using the Freeze button.
If the image is acceptable, press the CAPT. button to save the image.

(The operating procedure differs if DF/FL scene mode is selected. For a detailed discussion of
this topic, refer to the operating manual provided with the camera.)

23
 2 Part Names and Microscopy Procedures
(With Epi-illumination Attachment Mounted)

2.1 Names of Main Components

Camera head
(Required for image capture)
(One example shown in the photo)
Eyepiece tube
Photographic vertical tube adapter
Eyepiece
(Required for image capture)

Epi-illumination lamphouse
(The Epi-illumination
attachment uses a mercury,
Epi-illumination xenon, or high-intensity
attachment halogen lamp as its light
Revolving nosepiece source. It requires a separate
power supply unit. Before using
the lamphouse, be sure to read
the lamp operating manual.)
Objective
Lamphouse

Specimen holder
Stage

Condenser

Microscope (main body)

Camera control unit
(Required for image capture)

24
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.2 Names of Parts Used to Make Adjustments

2.2 Names of Parts Used to Make Adjustments

2.2.1 Right view 2

Camera cable

Camera head
C mount adapter

C mount adapter retaining screw

Optical path
Diopter adjustment switching lever
ring

Excitation method
switchover turret

Excitation ID tag pocket

Shutter open/close
lever
Filter cube mounting
section (inside)

Aperture diaphragm
lever

Y stage knob

X stage knob
(Provided on the left side
in certain products)

Filter IN/OUT switch

Fine focus knob

Coarse focus upper-limit clamp

Field diaphragm knob

Field diaphragm Aperture diaphragm

open/close lever (for open/close lever (for
Epi-fluorescent light) Epi-fluorescent light)

Field diaphragm Aperture diaphragm

centering holes centering holes

25
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.2 Names of Parts Used to Make Adjustments

2.2.2 Left view

Condenser focus knob

(Provided on Coarse focus torque

the right side adjustment knob
in certain
products) Coarse focus
knob
Condenser
Fine focus knob centering
screw

Brightness control knob Preset switch Power ON indicator

ND4 filter slider

ND8 filter slider

ND16 filter slider

Left side the Epi-illumination attachment

2.2.3 Rear view (without accessory device)

Tool storage compartment

Power switch
Lamphouse AC inlet
connector port

Input voltage indication

Lamphouse mounting section

26
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.3 Bright-Field Microscopy

2.3 Bright-Field Microscopy

1 Turn on power. 2

Press the power switch to

the "|" position.

2 Press the preset switch.

Press the preset switch.

3 Move the ND8, ND32, and

NCB11 filters into the optical
path.

NCB11
ND32
ND8

4 Push the optical path-switching

lever to direct the full optical
The full optical
path directed
toward binocular
Push in the optical
section
path toward the binocular path-switching lever.

section.

27
Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.3 Bright-Field Microscopy

5 Rotate the excitation method

switchover turret to set it at
position where no filter cube is
installed.

Rotate the excitation method

switchover turret.

6 Raise the condenser to the

uppermost position.

Raise the condenser using the

condenser focus knob.

7 Fully open the field diaphragm

and aperture diaphragm.
(When using the 1-100x condenser, move the top
lens into the optical path.)

Fully open the Fully open the field

aperture diaphragm diaphragm using the
using the aperture field diaphragm knob.
diaphragm lever.

8 Set the 10x objective into the

optical path.

Select the 10x

objective.

28
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.3 Bright-Field Microscopy

9 Set a specimen and move the

portion to be viewed into the
Set a specimen and secure in
place using the specimen holder.

optical path.
2

Move the portion to be viewed into the

optical path using the XY stage knobs.

10 Focus on the specimen.

Focus on the specimen using

the coarse and fine focus knobs.

11 Adjust the diopter and the

interpupillary distance.
Refer to "8. Diopter Adjustment" and "9.
Interpupillary Distance Adjustment" in the separate
Reference manual.

12 Focus and center the condenser.

Refer to "11. Adjustment of Condenser Position" in
the separate Reference manual.

Focus the condenser Center the condenser

using the condenser using the condenser
focus knob. centering screws.

Field diaphragm Field diaphragm

Eyepiece field of view Eyepiece field of view

29
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.3 Bright-Field Microscopy

13 Switch to the desired objective

and view the specimen.
Adjust the field diaphragm and
aperture diaphragm each time
you change objectives.
Select the desired objective.

Field diaphragm:
Set slightly narrower than the margins of
the field of view.

Aperture diaphragm:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective

Aperture diaphragm Field diaphragm knob

knob

14 Turn off power after completing

observations.

Press the power switch

to the "" position.

30
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.4 Epi-fluorescence Microscopy

2.4 Epi-fluorescence Microscopy

Before microscopy...

Check the cumulative operating hours of the lamp in the Epi-illumination

2
attachment. Replace the lamp if its cumulative operating hours exceed the
recommended maximum service life.

Use non-fluorescent slide glass.

Use non-fluorescent immersion oil.

To keep specimen colors from fading, keep the shutter closed when not
performing microscopy.

1 Perform steps 1 through 11 in "2.3 Bright-Field

Microscopy."

2 Lower the condenser, or remove the condenser and mount

the shielding tube in its place.

3 Turn off the microscope power

switch.

Press the power switch

to the "" position.

4 Close the shutter and block the

Epi-illumination light.

Close the shutter.

5 Move the filter cube for the

excitation method to be used
into the optical path.

Select a cube using the excitation

method switchover turret.

31
Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.4 Epi-fluorescence Microscopy

6 Fully open the field diaphragm

and aperture diaphragm of the
Epi-illumination attachment.

Fully open the field Fully open the

diaphragm. aperture diaphragm.

7 Switch on power for the light

source of the Epi-illumination
attachment, then open the
shutter and center the lamp.
(Refer to the operating manual
for the light source.) Open the shutter.

8 Set the 10x objective into the

optical path.

Select the 10x objective.

9 Set a specimen and move the

portion to be viewed into the
Set a specimen and secure in place
using the specimen holder.

optical path.

Move the portion to be viewed into the

optical path using the XY stage knobs.

32
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.4 Epi-fluorescence Microscopy

10 Focus on the specimen.

Focus on the specimen using

the coarse and fine focus knobs.

11 Center the field diaphragm of the

Epi-illumination attachment.
Field diaphragm
open/close lever

Refer to "4. Epi-fluorescent Field Diaphragm" in

"17. Fluorescence Observation" of the separate
Reference manual.
Field diaphragm
centering screw holes

Field
diaphragm
image

Eyepiece
field of view

12 Switch to the desired objective

and view the specimen.
Use the ND filters for the fluorescence
attachment to adjust brightness.

Adjust the field diaphragm so that it extends

slightly beyond the field of view.
Select the desired
The image brightness can be adjusted with objective.
the aperture diaphragm. Complete centering ND4 filter slider
before using the aperture diaphragm.
ND8 filter slider
When using oil immersion objectives, apply
immersion oil between the specimen and the ND16 filter slider
objective.

33
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.4 Epi-fluorescence Microscopy

13 To return to bright-field
microscopy
Close the shutter of the Epi-illumination
attachment and block the epifluorescent light.

Rotate the excitation method switchover turret

to move the position where no fluorescent
Close the shutter.
cube is installed into the optical path.
Use the excitation method switchover
If the condenser is not mounted, install the turret to change cubes.
condenser, then focus and center the
condenser. (Refer to step 12 of bright-field
microscopy.)

Turn on the microscope power switch to turn

on the diascopic light source.

Press the switch to the

"|" position.

14 Turn off all power switches after

completing observations.

Press the power switch

to the "" position.

34
 Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.5 Photomicroscopy

2.5 Photomicroscopy

For detailed discussions of the camera, photomicroscopic software, and PC, refer to the
operating manuals provided with the respective products. The following instructions assume a
2
DS-5M digital camera and DS-L1 camera control unit.

1 Adjust the microscope for proper image observation.

See the directions given in previous "1. Bright-Field Microscopy" or "2. Epi-fluorescence
Microscopy."

2 Adjust the camera head mounting position until the image is

displayed properly.
(1) Adjustment based on stage motion
directionLoosen the C-mount adapter
retaining screw and adjust the camera
position so that moving the stage
forward-backward or left-right moves the
image on the monitor in the same direction.
After making the appropriate adjustments,
tighten the screw firmly.
(B)

(A)

Adjusting (A) and (B)

C-mount
adapter
retaining
screw

35
Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)

2.5 Photomicroscopy

3 Make camera settings.

For a detailed discussion of this topic, refer to the operating manual provided with the camera.
When using the DS-L1, you must choose and enter at least the following information:

Folder for data storage.

Name of file to be saved. (You can select "Auto.")

File format and file size.

Date and destination of data

4 Select the camera scene mode suitable for the microscopy

method.

5 Set the camera white balance.

To adjust white balance, press the WB button while capturing an image of a clear section of a
specimen slide. (For fluorescent photomicrography, adjust white balance under normal lighting
conditions before shooting.)

6 Capture and save images.

Focus on the specimen.
Refocus.
Adjust image brightness using the camera exposure compensation function.
Check the image using the Freeze button.
If the image is acceptable, press the CAPT. button to save the image.

(The operating procedure differs if DF/FL scene mode is selected. For a detailed discussion of
this topic, refer to the operating manual provided with the camera.)

36
3 Part Names and Microscopy Procedures
(With DIH-M Mounted)

3.1 Names of Main Components

Camera head
(Required for image capture)
(One example shown in the photo)

Eyepiece
3
DIH-M

Epi-illumination lamphouse
(The Epi-illumination attachment
uses a mercury, xenon, or
high-intensity halogen lamp as
its light source. It requires a
Revolving nosepiece
separate power supply unit.
Before using the lamphouse, be
Objective sure to read the lamp operating
manual.)

Specimen holder Lamphouse

Stage
Condenser

Microscope
(main body) Camera control unit
Shutter open/close
(Required for image capture)
hand switch for
(One example shown in the photo)
Epi-fluorescent light

37
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.2 Names of Parts Used to Make Adjustments

3.2 Names of Parts Used to Make Adjustments

3.2.1 Right view

C mount adapter

C-mount adapter retaining screw

Optical path
Camera cable
switching lever (1)

Optical path Camera head

switching lever (2)

Diopter adjustment
ring

Zoom knob
Excitation method
switchover turret

Excitation ID tag pocket

Filter cube mounting

section
(inside)

Aperture diaphragm lever

Y stage knob
X stage knob
(Provided on the left side
in certain products)
Filter IN/OUT
switch

Fine focus knob

Coarse focus upper-limit clamp

Field diaphragm knob

Field diaphragm Aperture diaphragm

open/close lever (for open/close lever (for
Epi-fluorescent light) Epi-fluorescent light)

Field diaphragm Aperture

centering holes diaphragm
centering holes

38
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.2 Names of Parts Used to Make Adjustments

3.2.2 Left view

DIH-M Power ON indicator

ND4 filter slider

ND8 filter slider
ND16 filter slider

Condenser focus knob

(Provided on Coarse focus torque

the right side adjustment knob
in certain
products) Coarse focus
knob
Condenser
Fine focus knob centering
screw

Brightness control knob Preset switch Power ON indicator

39
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.2 Names of Parts Used to Make Adjustments

3.2.3 Rear view (without accessory device)

Tool storage compartment

C-Box mounting sections

Power switch
Lamphouse AC inle
connector port

Input voltage indication

Lamphouse mounting section

C-Box power
switch

Intelligent nosepiece
Not used
connector

DIH-M
Connector for connector
Epi-illumination
shutter open/close
hand switch

AC adapter inlet

Outside the C-Box Inside the C-Box

40
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.3 Bright-Field Microscopy

3.3 Bright-Field Microscopy

1 Turn on power.
The C-Box and the power source for the
Epi-illumination do not need to be turned on. If
they are turned on, keep the shutter of the
Epi-illumination closed.
Press the power switch to
3
(The shutter is automatically kept closed when the
power switch is turned on. Do not touch the hand the "|" position.
switch. If the shutter is opened accidentally, press
the hand switch once again to close the shutter.)

2 Press the preset switch.

Press the preset switch.

3 Move the ND8, ND32, and NCB11

filters into the optical path.

NCB11
ND32
ND8

4 Pull out both optical path

switching levers and direct the
The full optical
path directed
toward the
Pull out both upper and
lower optical path
switching levers.
binocular
full optical path toward the section
binocular section.

41
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.3 Bright-Field Microscopy

5 Rotate the excitation method

switchover turret to set it a
position where no fluorescent
cube is installed.

Rotate the excitation method

switchover turret.

6 Raise the condenser to the

uppermost position.

Raise the condenser using the

condenser focus knob.

7 Fully open the field diaphragm

and aperture diaphragm.

Fully open the aperture Fully open the field

diaphragm using the diaphragm using the
aperture diaphragm field diaphragm knob.
lever.

8 Set the 10x objective into the

optical path.

Select the 10x objective.

42
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.3 Bright-Field Microscopy

9 Set a specimen and move the

portion to be viewed into the
Set a specimen and secure in
place using the specimen holder.

optical path.

3
Move the portion to be viewed into the
optical path using the XY stage knobs.

10 Focus on the specimen.

Focus on the specimen using

the coarse and fine focus knobs.

11 Adjust the diopter and the

interpupillary distance.
Refer to "8. Diopter Adjustment" and "9.
Interpupillary Distance Adjustment" in the separate
Reference manual.

43
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.3 Bright-Field Microscopy

12 Focus and center the condenser.

Refer to "11. Adjustment of Condenser Position" in
the separate Reference manual.

Focus the condenser using Center the condenser

the condenser focus knob. using the condenser
centering screws.

Field diaphragm Field diaphragm

Eyepiece field of view Eyepiece field of view

13 Switch to the desired objective

and view the specimen.

Adjust the field diaphragm and

aperture diaphragm each time
you change objectives.
Field diaphragm:
Select the desired objective.
Set slightly narrower than the margins of
the field of view.

Aperture diaphragm:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective

Aperture diaphragm Field diaphragm knob

lever

14 Turn off power after completing

observations.

Press the power switch

to the "" position.

44
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.4 Epi-fluorescence Microscopy

3.4 Epi-fluorescence Microscopy

Before microscopy...

Check the cumulative operating hours of the lamp in the Epi-illumination

attachment. Replace the lamp if its cumulative operating hours exceed the
recommended maximum service life.

Use non-fluorescent slide glass.

3
Use non-fluorescent immersion oil.

To keep specimen colors from fading, keep the shutter closed when not
performing microscopy.

1 Perform steps 1 through 11 in "3.3 Bright-Field

Microscopy."

2 Lower the condenser, or remove the condenser and mount

the shielding tube in its place.

3 Turn off the microscope power

switch.

Press the power switch

to the "" position.

4 Move the filter cube for the

excitation method to be used
into the optical path.

Select a cube using the excitation

method switchover turret.

5 Fully open the field diaphragm

and aperture diaphragm of the
Epi-illumination.

Push to fully open Push to fully open

the field diaphragm. the aperture

45
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.4 Epi-fluorescence Microscopy

6 Switch on power for the C-Box

and Epi-illumination light source.

(Note that the shutter for the C-Box

Epi-illumination is closed power switch

automatically.)

7 Press the hand switch to open

the shutter of the
Push to open the shutter.

Epi-illumination and center the

lamp. (Refer to the lamp
operating manual.)

8 Set the 10x objective into the

optical path.

Select the 10x objective.

9 Set a specimen and move the

portion to be viewed into the
Set a specimen and secure in place
using the specimen holder.

optical path.

Move the portion to be viewed into the

optical path using the XY stage knobs.

46
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.4 Epi-fluorescence Microscopy

10 Focus on the specimen.

Focus on the specimen using the 3

coarse and fine focus knobs.

11 Center the field diaphragm of the

Epi-illumination attachment.
Field diaphragm
open/close lever

Refer to "4. Epi-fluorescent Field Diaphragm" in

"17. Fluorescence Observation" of the separate
Reference manual.
Field diaphragm centering
screw holes

Field
diaphragm
image

Eyepiece
field of view

12 Switch to the desired objective

and view the specimen.
Use the ND filters for the fluorescence
attachment to adjust brightness.

Adjust the field diaphragm so that it extends

slightly beyond the field of view.

The image brightness can be adjusted with

Select the desired objective.
the aperture diaphragm. Complete centering
before using the aperture diaphragm.

When using oil immersion objectives, apply

immersion oil between the specimen and the
objective.

ND16 filter slider

ND8 filter slider
ND4 filter slider

47
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.4 Epi-fluorescence Microscopy

13 To return to bright-field
microscopy.
Close the shutter of the Epi-illumination and
block the Epi-illumination.

Rotate the excitation method switchover

turret to move the position where no filter
cube is installed into the optical path. Use the excitation method switchover
turret to change cubes.
If the condenser is not mounted, install the
condenser, then focus and center the
condenser. (Refer to step 12 of bright-field Close the shutter.
microscopy.)

Turn on the microscope power switch to turn

on the diascopic light source.

Press the switch to the

"|" position.

14 Turn off all power switches after

completing observations.

Press the power switch

to the "" position.

48
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.5 Photomicroscopy

3.5 Photomicroscopy

For detailed discussions of the camera, photomicroscopic software, and PC, refer to the
operating manuals provided with the respective products. The following instructions assume a
DS-5M digital camera and DS-L1 camera control unit.

1 Adjust the microscope for proper image observation. 3

See the directions given earlier in "1. Bright-Field Microscopy" or "2. Epifluorescence
Microscopy."

2 Adjust the camera head mounting position until the image is

displayed properly.
(1) Adjustment based on stage motion direction
Loosen the C-mount adapter retaining screw
and adjust the camera position so that
moving the stage forward-backward or
left-right moves the image on the monitor in
the same direction. After making the
appropriate adjustments, tighten the screw
firmly. (B)

(A)

Adjusting (A) and (B)

C-mount adapter
retaining screw

3 Make camera settings.

For a detailed discussion of this topic, refer to the operating manual provided with the camera.
When using the DS-L1, you must choose and enter at least the following information:

Folder for data storage

Name of file to be saved (You can select "Auto.")

File format and file size

Date and destination of data

49
 Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.5 Photomicroscopy

4 Select the camera scene mode suitable for the microscopy

method.

5 Set the camera white balance.

To adjust white balance, press the WB button while capturing an image of a clear section of a
specimen slide. (For fluorescent photomicrography, adjust white balance under normal lighting
conditions before shooting.)

6 Capture and save images.

Adjust image brightness using the camera exposure compensation function.
Check the image using the Freeze button.
If the image is acceptable, press the CAPT. button to save the image.

(The operating procedure differs if DF/FL scene mode is selected. For a detailed discussion of
this topic, refer to the operating manual provided with the camera.)

50
4 Assembly

1 Checking the Input Voltage

Check the input voltage indicated on the back of
the microscope. Use the microscope only if this
indication matches the power supply voltage for the
area in which the microscope will be used.
Input voltage
Note: If the voltage indication and supply voltage
indication
differ, do not attempt to use the microscope.
Contact your nearest Nikon representative to seek
advice.
Back

2 Installing a Lamp
Lamphouse-cover fixing screw
(1) Loosen the lamphouse-cover fixing screw and
lift the lamphouse cover to remove.

(2) Attach the lamp to the lamp plug.

Avoid touching the bare lamp bulb with your
bare hands.
Use the lamp type specified. Using an
unspecified lamp may cause malfunction or
fire. Refer to Chapter 8 for the specified lamp.

(3) Replace the cover and tighten the screw.

Lamp
plug

CAUTION

The lamphouse cover must be attached. Failure to replace the lamphouse cover
may result in burns or fire from the heat generated by the lamp.

51
 Chapter 4 Assembly

3 Attaching the lamphouse Attaching the lamphouse

(1) Attach the lamphouse to the back of the

microscope and fix it with the fixing screw.

(2) Attach the connector.

Fixing screw

Attaching the connector

4 Installing a Stage
(1) Turn the coarse focus knob to remove the
cushioning material from the elevating
section.

Turn this to lower the elevating stage.

52
 Chapter 4 Assembly

(2) Place the stage on the elevating section and

fix into place with two screws.
(For the stage with a centering function, place
the stage on the elevating section by aligning
the projection on the back of the stage with
the groove of the elevating section.)

Fixing the stage

Screws fixing the stage

4

Groove

Stage with centering function

Projection

Back of the stage with centering function

(3) Place a specimen holder on the stage and

secure with screws.
Fixing the stage with centering function

Fixing the specimen holder

53
 Chapter 4 Assembly

5 Installing a Condenser Condenser focus knob

(1) Turn the coarse focus handle until the

elevating section is raised to the highest
position.

(2) Turn the condenser focus knob until the

elevating section is brought to the lowermost
position.

(3) Insert a condenser and adjust so that it faces

toward the front. Secure in place with a tool
stored in the back of the microscope. Fixing condenser

(4) Turn the condenser focus knob until the

elevating section is raised to the uppermost
position.

6 Installing an Eyepiece Tube directly

(1) Place an eyepiece tube on the microscope
arm.

(2) Attach the eyepiece by aligning the projection

of the eyepiece tube sleeve with the notch at
the objective.

(3) Attach a screw hole protection sticker

provided with the system to the bolt hole
Fixing the eyepiece tube
found on the top surface of the arm.

Projection Notch

Eyepiece attached in place

54
 Chapter 4 Assembly

7 Installing an Epi-illumination attachment

Screws to fix the Epi-illumination
(1) Place an Epi-illumination attachment on the
attachment (three screws in total)
microscope arm and fix it with a screw at the
front of the arm.

(2) Secure in place with two screws at the back of

the Epi-illumination attachment.

(3) Attach a screw hole protection sticker

provided with the system to the bolt hole.

(4) Attach a mercury lamphouse to the bayonet

mount on the back. (For more information,
refer to the instruction manual provided with
the super high-pressure mercury lamp power
Bayonet mount 4
supply.)

1) Attach a collector lens to the lamphouse.

2) Turn the bayonet mount clockwise (viewed

from the back) as far as it will go.

3) Insert the lamphouse into position.

4) Turn the bayonet mount anticlockwise

(viewed from the back) to lock the
lamphouse.
Mercury
5) Attach a mercury lamp. Eyepiece Eyepiece tube
lamphouse
6) Connect the mercury lamphouse to the
mercury lamp power supply.

(5) Attach the eyepiece tube.

(6) Attach the eyepiece by aligning the projection

of the eyepiece tube sleeve with the notch at
the objective.

Eyepiece tube fixing screw

Projection Notch

Eyepiece attached in place

55
 Chapter 4 Assembly

8 Using the DIH-M

(1) Attach two C-BOXs to the back of the
microscope with screws.
C-BOX
fixing

(2) Place the DIH-M on the microscope arm and

fix it with a screw at the front of the arm. Cover fixing screw

(3) Remove the back cover of the DIH-M by

loosening the two screws.

(4) Fix the DIH-M to the microscope with two

screws inside the arm.

DIH-M fixing screw (Three positions)

(5) Connect the ferrite core side of the control Control cable
cable to the CTL connector. When inspecting
DIH-M conditions, also attach the USB cable.
USB cable (Always
In this case, the other end of the USB cable is connect this cable
connected to the PC. when inspecting
(The control cable and the USB cable are DIH-M conditions.)
provided with the DHI-M.)
Interlock connector
Ferrite core for C1 (Used only
with C1 attached)
(6) Replace the cover.

For maintenance
(not used)

Viewed from the

other side

56
 Chapter 4 Assembly

(7) Connect the control cable attached to the Fixing screws for optical
path switching section
DIH-M to the C-BOX.

1) Remove the cover on the left side of the

DIH-M.

2) Loosen the optical path switching section

fixing board mount screw.

3) Loosen the two optical path switching

section fixing screws and remove the clamp Fixing board for Mount screw for fixing
optical path board for optical path
from the inside of the optical path switching
switching section switching section
section. (This frees the optical path
switching lever.) Cover

4) Move the fixing board slightly forward with

the fixing screws attached, then tighten the
Blind cap 4
mount screw.

5) Replace the cover.

6) Loosen the zooming section fixing screw to

remove the clamp from the DIH-M.

7) Attach the blind cap provided. Fixing screw for

zooming section

(8) Connect the control cable attached to the

DIH-M to the C-BOX.

Connect the control

cable attached to the
DIH-M here.

(9) Attach the hand switch for the

Epi-illumination shutter to the C-BOX.

Attach the hand switch for

the Epi-illumination shutter
here.

57
 Chapter 4 Assembly

(10) Attach the mercury lamphouse to the back of

Bayonet mount
the bayonet mount. (For more information,
refer to the instruction manual provided with
the super high-pressure mercury lamp power
supply.)

1) Attach a collector lens to the lamphouse.

2) Turn the bayonet mount clockwise (viewed
from the back) as far as it will go.
3) Insert the lamphouse into position.
4) Turn the bayonet mount anticlockwise Mercury lamphouse
Objective
(viewed from the back) to lock the
lamphouse.
5) Attach a mercury lamp.
6) Connect the mercury lamphouse to the
mercury lamp power supply.

Projection Notch
(11) Attach the eyepiece by aligning the projection
of the eyepiece tube sleeve with the notch at
the objective.

Eyepiece attached in place

58
 Chapter 4 Assembly

9 Installing a Revolving Nosepiece

Fixing the revolving nosepiece
(1) Lift the revolving nosepiece from a position
just forward of the point directly below the
fitting part and slide toward the back to
attach. (Continue sliding the revolving
nosepiece until its front position is aligned
with that of the fitting part.)

(2) Secure with the screw.

4
(3) When using the intelligent revolving
nosepiece, connect the connector attached to
the revolving nosepiece to the upper port Connect the connector
here when using the
found inside the C-BOX.
intelligent revolving
nosepiece.

10 Installing Objectives
Screw objectives into the revolving nosepiece. When installing the objective in this way, make
sure that the magnification of the objective increases when the revolving nosepiece is turned
clockwise (clockwise when viewed from above the eyepiece).

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 Chapter 4 Assembly

11 Installing Filter Cubes and a Light Shield (when the

Epi-illumination attachment or the DIH-M is installed)
(1) There is a front cover below the eyepiece. Pull Address
out the cover to remove. Excitation switching turret
indication

(2) Insert a filter cube into position.

Filter cube
Filter cube fitting part

(3) Insert a nameplate into the position with the

Address Address
same address as the one indicated on the filer
indication indication
cube select knob on the right side of the
microscope.

(4) Turn the filter cube select knob and insert a

filter cube into the remaining open position.

(5) Replace the cover.

Nameplate

(6) Attach a light shield to the front bottom of the

fluorescent unit with screws.

Light shield attached Light shield attached

(with DIH-M) (with Epi-illumination attachment)

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 Chapter 4 Assembly

12 Installing a Camera (when using a camera)

12-1 When attaching to the ergonomic binocular tube:

(1) To attach a camera head, screw it into the C Camera head
mount on the DSC port.

(2) Remove the rear cover of the ergonomic

binocular tube and insert the DSC port.

(3) Secure the DSC port into place with screws.

Securing the DSC port

DSC port 4
Camera cable
(4) Attach the camera cable to the camera head. fitting part
(Adjust the fitted position before using the
camera. Refer to 2.4 operation procedure.)

Camera
centering screw
Camera focus position
adjusting screws

12-2 When attaching to the trinocular eyepiece tube:

C-mount
(1) Attach the camera head to the trinocular adapter fixing
eyepiece tube using the C-mount adapter. screw

(2) Attach the camera cable to the camera head.

(Adjust the fitted position before using the
camera.)

12-3 When attaching the DIH-M: C-mount adapter

(1) Attach the camera head to the DIH-M using

the C-mount adapter that is near the rear
port.

(2) Attach the camera cable to the camera head.

(Adjust the fitting position before using the
camera.)

Fixing the C-mount adapter

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 Chapter 4 Assembly

13 Installing the Power Cord

(1) Check to confirm that the microscope power
switch is off.

(2) Insert one end of the power cord into the AC

inlet at the back of the microscope.
Power switch for the
(3) Insert the other end of the power cord into microscope
the wall outlet.
AC inlet
Use only the specified power cord.
Refer to Chapter 8 for the power cord
specified. Right side of the back

14 Connecting the AC Adapter (when using the DIH-M)

(1) Confirm that the power switch of the C-BOX
(attached to the back of the microscope) is off
(the button projects out). C-BOX power
switch

(2) Connect the AC adapter to the bottom port on Connect the

the front of the C-BOX. AC adapter
here.

(3) Connect the AC adapter to the wall outlet with

the power cord.

Use only the AC adapter and power cord

specified.
Refer to Chapter 8 for the specified AC
adapter and power cord.

Microscope assembly is now complete.

62
5 Replacing Consumables

5.1 Replacing the lamp

CAUTION

Be careful to avoid burns:

Wait until the lamp and nearby parts have cooled before attempting to replace
the lamp.

Be careful to avoid electrical shock:

Turn off the power switch and unplug the power cord from the outlet.

Be careful to avoid abnormal heat generation:

Use only the lamp specified.

Be careful to avoid actions that might reduce lamp service life:

Avoid touching the bare lamp bulb with bare hands. Soiling will reduce the
service life of the lamp.
5
(1) Loosen the screw and lift the cover to
remove. Lamphouse cover fixing screw

(2) Remove the old lamp.

(3) Replace with a new lamp.

Avoid touching the bare lamp bulb with bare
hands.
Use only the lamp specified. Refer to chapter
8 for the specified lamp.

(4) Replace the cover and fix it with the screw.

Lamp
plug

CAUTION

The lamphouse cover must be attached. Failure to replace the lamphouse cover
may result in burns or fire from the heat generated by the lamp.

63
 6 Troubleshooting

If the microscope does not function property, take appropriate action as described below. If the
problem is still not resolved after referring to "Troubleshooting," please contact your nearest
Nikon representative

6.1 Optical

Problem Possible causes Remedy

Install the Parts (nosepiece, condenser,
Parts installed incorrectly field diaphragm unit, aperture diaphragm
unit, etc.) correctly.
Switch the parts (optical path switching
lever, excitation method switching turret,
Movable parts not switched correctly nosepiece, filter slider, condenser turret,
View field vignetting etc.) correctly. (Operate the parts to a
Uneven illumination position until you feel resistance.)
across the view field Adjust the condenser so that the field
View field not visible Condenser adjusted in correctly diaphragm image is shown in the center
of the viewing field.
Improper objective-condenser
Use a proper combination.
combination
Install the lamp securely and adjust it as
Lamp installed incorrectly
necessary.
Field diaphragm not sufficiently Open the field diaphragm to the proper
opened setting.
Dirt and dust on lens, condenser,
Clean.
eyepiece lens, filter and specimen
Dirt or dust in the field Field diaphragm image not focused
Focus and center the condenser.
of view on the specimen surface
Open the field diaphragm to the proper
Field diaphragm closed too far
setting.
Field diaphragm closed or opened Open or close the field diaphragm to the
too far proper setting
Objective correction ring not Adjust the correction ring to match the
adjusted cover glass.
Adjust the condenser so that the field
Condenser maladjusted diaphragm image is shown in the center
of the viewing field.

Poor image quality Cover glass unattached Attach a cover glass of the specified
Poor contrast Cover glass thickness not as specified thickness (0.17 mm).

Poor resolution Tip pf the oil-immersed objective

not immersed in oil Use Nikon immersion oil.
Unspecified immersion oil used
Air bubbles in immersion oil Remove the air bubbles.
Immersion oil attached to tip of the
Clean.
dry objective
Dirt or dust on lens, condenser, eye
Clean.
piece lens, filter and specimen
Revolving nosepiece not installed
Install correctly and rotate to the click
correctly, or not rotated to the click
stop position.
Uneven focus stop position
Specimen projecting from the stage Securely attach the specimen to the
surface stage specimen holder.

64
 Chapter 6 Troubleshooting

6.2 Electrical

Problem Possible causes Remedy

Revolving nosepiece not installed
Install correctly and rotate to the click
correctly, or not rotated to the click
stop position.
stop position
Image flows Specimen projecting from the stage Securely attach the specimen to the
surface stage specimen holder.
Install correctly and rotate to the click
Condenser tilted
stop position.
NCB11 filter not being used Use the NCB11 filter.
Yellow-tinged
Lamp voltage too low Press the Preset switch and adjust the
View field too bright Lamp voltage too high brightness to match the ND filter
Lamp voltage too low combination.

Condenser aperture diaphragm Open the aperture diaphragm to the

stopped down too far proper setting.
Adjust the condenser so that the field
View field too dark Condenser maladjusted diaphragm image is shown in the center
of the viewing field.
Adjust the light path switching lever to
Image light spread over too great
provide the target location with 100%
an area
light.
Attach the specimen to the stage with the
Focus impossible on a
Specimen upside down
cover glass face up. 6
high-magnification Attach a cover glass of the specified
Unspecified cover glass thickness
objective thickness (0.17 mm).
Diopter not adjusted Adjust the diopter.
Too great a difference
in focus after objective Diopter not adjusted Adjust the diopter.
switching.
Specimen not moving Specimen holder insufficiently Attach the specimen holder securely to
smoothly attached to stage the stage.
Images on the left and Interpupillary distance incorrect Make adjustment
right eyepiece not
coincident Diopter not adjusted Adjust the diopter.

6.2 Electrical

Problem Possible causes Remedy

No power despite power Power cord not connected or
Connect properly.
switch turned on connected improperly
Lamp burned out Replace with specified lamp.
Lamp does not light
Impossible to install the lamp Replace with specified lamp.
Lamp used is
Lamp burns out quickly Replace with specified lamp.
unspecified/incompatible
Microscope brightness
control knob not Preset switch off If off, switch it on.
functional

65
7 Cleaning and Maintenance

7.1 Lens cleaning

Keep the lens free of dust, fingerprints, etc. Dirt on the lenses or filters will affect image quality.
If any of the lenses become dirty, clean them by the procedure given below.

Brush away dust with a soft brush or wipe away gently with gauze.

If fingerprints or grease gets on a lens, moisten a piece of soft, clean cotton cloth, lens
tissue, or gauze with absolute alcohol (ethyl or methyl alcohol) and wipe.

Use petroleum benzine only to remove immersion oil from the objective. For optimum
results, we recommend following up petroleum benzine with absolute alcohol (ethyl or
methyl alcohol). If petroleum benzine is unavailable, use methyl alcohol alone. When
using just methyl alcohol, note that surfaces will need to be wiped repeatedly to ensure
complete removal of immersion oil. Usually, three or four times should be sufficient to
clean the lens.

Never use petroleum benzine to clean the entrance lens at the bottom of the eyepiece
tube or prism surface of the eyepiece tube.

Absolute alcohol and petroleum benzine are highly flammable. Be careful when handling
these materials, particularly around open flames or when turning the power switch on or
off.

Follow the instructions provided by the manufacturer when using absolute alcohol.

7.2 Cleaning the product

We recommend using a silicon cloth to clean the microscope.

For stubborn dirt, dampen a piece of gauze with neutral detergent and wipe gently.

Use of organic solvents on plastic parts may result in discoloration.

7.3 Disinfecting the product

For routine disinfection of the microscope, we recommend using 70% medical alcohol.

If contact occurs between a sample and the microscope, determine whether the sample is
hazardous. If the sample is hazardous, follow the standard procedures for your
laboratory.

Use of organic solvents on plastic parts may result in discoloration.

66
 Chapter 7 Cleaning and Maintenance

7.4 Storage

7.4 Storage

Store the microscope in a dry location where mold is unlikely to form.

Store the objectives and eyepieces in a dry box or similar container with a drying agent.

Place the vinyl cover over the microscope to protect it from dust.

Switch off the microscope (press the switch to the "O" position) and wait for the
lamphouse to cool before covering the microscope with the vinyl cover.

7.5 Periodic inspections (fee charged)

To maintain the peak performance of the microscope, we recommend periodic inspections.

Contact your nearest Nikon representative for more information. (Parts and service charges
apply for this service.)

67
 8 Specifications

8.1 Specifications

Nikon Microscope ECLIPSE 80i

Model ECLIPSE 80i

Main unit

Optical system CFI60 system (infinity-corrected CF optical system)

Main unit T-shaped, double-winged

Illumination system Flyeye illumination, power supply incorporated, with preset feature

12V/100W long-life halogen lamp

(specified lamp housing: D-CH precentered lamp housing)

(specified lamp: PHILIPS7724I or OSRAM HLX64623)

NCB11, ND8, ND32 incorporated (replaceable)

Focusing section Manual single-axis coarse/fine motion handle system (right coarse/fine motion, left
coarse motion, calibration markings for fine motion: 1 m/marking)

Stroke: 27 mm, with refocusing mechanism

Coarse motion handle: 14mm/revolution, fine motion handle: 0.1 mm/revolution

Tube section Binocular tube, ergonomic binocular tube (binocular section 100%, binocular: DSC
port = 50:50)

DSC port mountable to ergonomic binocular tube

Trinocular eyepiece tube FUW (binocular: straight = 100:0, 0:100)

Trinocular eyepiece tube TUW (binocular: straight = 100:0, 20:80, 0:100)

Nosepiece section Sextuple nosepiece, sextuple DIC nosepiece, intelligent septuple nosepiece,
intelligent septuple DIC nosepiece

Stage section Vertical stage (vertical stage R: main centering unit)

Condenser section Abbe condenser, achromat condenser, darkfield condenser (dry, oil)
Phase contrast condenser (cannot be attached to main centering unit), achromatic
aplanat condenser, LWD condenser
1-100X condenser, DIC condenser (oil), universal condenser (dry)

Eyepiece lens section 10x, field number: 22, 25

Input ratings 100-240V AC, 10%, 50/60Hz, 2.4A max.

AC adapter for C-BOX Manufacturer: ILAN ELECTRONICS LTD.

Model: F1650K

Rated input voltage: 100V-240V AC, 1.2A max., 50-60Hz

Rated input voltage: 12V DC, 3.5A max

Other: UL-listed, GS-approved, CE-certified

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 Chapter 8 Specifications

8.1 Specifications

Power cord 100-120V regions

UL-listed detachable power cord set (3 conductor grounding Type SVT, AWG 18,
3m long maximum, rated at 125V AC minimum)

230V areas
EU/EN-approved 3-conductor power cord set (3 conductor grounding Type
H05VV-F, AWG 18, 3m long maximum, rated at 250V AC minimum)

Operating conditions Temperature: 0 to 40C

Humidity: 85% RH max. (no condensation)

Altitude: 2000 m max.

Degree of pollution: Degree 2

Installation: Category II

Electric shock protection class: Class I

Indoor use only

Transport/storage Temperature: -20 to 60C

conditions
Humidity: 90% RH max. (no condensation)

External dimensions and External dimensions: 300 (W) x 338 (H) x 384 (D) mm
weight (main unit)
Weight: Approx. 12 kg

Safety standards UL-Listed Product (UL61010A-1)

When using with the Meets FCC 15B Class A requirements.

following attachments:
This equipment has been tested and found to comply with the limits for a Class A
D-LH digital device, pursuant to Part 15 of the FCC Rules.
precentered These limits are designed to provide reasonable protection against harmful
lamphouse interference when the equipment is operated in a commercial environment.

This equipment generates, uses, and can radiate radio frequency energy and, if not
D-DH digital
8
installed and used in accordance with the instruction manual, may cause harmful
imaging head M interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful

D-CB C-BOX
interference in which case the user will be required to correct the interference at his
own expense.
D-NID6 sextuple
DIC nosepiece This class A digital apparatus complies with Canadian ICES-003.
Cet appreil numrique de classe A est conforme la norme NMB-003 du Canada
C-HS hand switch
(NMB-003).
Specified adapter
This group1 class B digital apparatus complies with Australian EMC
for C-Box
(AS/NZS2064).

CE Marking
Meets EU IVDD (In vitro diagnostic medical device Directive) requirements.
(GM-approved: In vitro diagnostic medical device)

EMC
Meets EU EMC Directive (EN61326) requirements.

69
 Chapter 8 Specifications

8.1 Specifications

D-DH Digital Imaging Head M for Nikon Microscopes

Model D-DH Digital Imaging Head M

Optical system Infinity-corrected CF optical system

Eyepiece lens field number: 25mm

Variable intermediate magnification:

Binocular tube 1X

Front port 1x

Rear port 0.8 to 2x (zoom ratio: 2.5, manual, with intelligent feature)

Light path switching Manual 3-way (eyepiece section: 100%, front port 100%, rear port 100%) (with
intelligent feature)

Eyepiece tube top viewing 25

angle

Fluorescent turret Manual sextuple turret (with noise terminator, intelligent feature)

Aperture stop Manual (unit detachable)

Field stop Manual (unit detachable)

ND filter Manual, 3 filters (slider type filters ND4, ND8, ND16)

Shutter Motorized (controlled with external hand switch)

Analyzer slot Yes (side surface)

Polarizer slot Yes (side surface)

Compatible lamp housing Hg, Xe, centered halogen (incompatible with precentered type)

External connection USB

C1 interlock

D-CB C-BOX

COM (for maintenance purposes)

Operating conditions Temperature: 0 to 40C

Humidity: 85% RH max. (no condensation)

Altitude: 2000 m max.

Degree of pollution: Degree 2

Installation: Category II

Indoor use only

Transport/storage Temperature: -20 to 60C
conditions
Humidity: 90% RH max. (no condensation)
External dimensions and External dimensions: 172 (W) x 185 (H) x 521 (D) mm (excluding projections)
weight
Weight: Approx. 8 kg

70