1. The document describes a procedure for extracting DNA from a banana. The objective is to learn the protocol for DNA extraction and become familiar with the principles.
2. The materials needed include a banana, blender, salt, detergent containing EDTA, alcohol, beakers, pipettes, and filter paper.
3. The procedure involves blending banana with water, adding detergent and salt solution to help break cell membranes, filtering to separate DNA from other materials, and precipitating the DNA out of solution using cold alcohol.
1. The document describes a procedure for extracting DNA from a banana. The objective is to learn the protocol for DNA extraction and become familiar with the principles.
2. The materials needed include a banana, blender, salt, detergent containing EDTA, alcohol, beakers, pipettes, and filter paper.
3. The procedure involves blending banana with water, adding detergent and salt solution to help break cell membranes, filtering to separate DNA from other materials, and precipitating the DNA out of solution using cold alcohol.
1. The document describes a procedure for extracting DNA from a banana. The objective is to learn the protocol for DNA extraction and become familiar with the principles.
2. The materials needed include a banana, blender, salt, detergent containing EDTA, alcohol, beakers, pipettes, and filter paper.
3. The procedure involves blending banana with water, adding detergent and salt solution to help break cell membranes, filtering to separate DNA from other materials, and precipitating the DNA out of solution using cold alcohol.
1. The document describes a procedure for extracting DNA from a banana. The objective is to learn the protocol for DNA extraction and become familiar with the principles.
2. The materials needed include a banana, blender, salt, detergent containing EDTA, alcohol, beakers, pipettes, and filter paper.
3. The procedure involves blending banana with water, adding detergent and salt solution to help break cell membranes, filtering to separate DNA from other materials, and precipitating the DNA out of solution using cold alcohol.
Objective 1. Learn the protocol of extracting DNA 2. Be familiar with the principles behind extracting DNA
Materials Banana Distilled water Blender 91% isopropyl alcohol (chill inside ref or ice bath) Graduated cylinder NaCl (12%) (3pcs) Beaker (250 ml) Pipettes Stirring rod Filter paper or cheesecloth Water bath Liquid detergent containing EDTA(colorless)
Procedure 1. Put 100 ml of distilled water and one banana into the blender. Blend for 25 seconds, making sure the banana is completely pulverized. Pour the mixture into a beaker. 2. Mix 10 ml of liquid detergent with 1ml of salt in a beaker. Add 10 ml of distilled water. Stir gently to avoid creating foam. Continue for a few minutes until the soap and salt are dissolved. 3. Add 2 tablespoons of the banana mixture to the beaker containing the soap solution. Stir the mixture GENTLY for at least 10 minutes. 4. Pour the mixture from step 3 into the filter. After 10 minutes, some liquid, called the filtrate, should have collected in the bottom of the cup. Gently stir the mixture in the filter and let it sit for another minute. Remove the filter and set it aside. 5. Get a test tube of cold alcohol. Add it to the mixture. Let it sit undisturbed for about four minutes. Do not shake. A white material will precipitate. 6. Compare your procedure with virtual laboratory. Visit the following website http://vlab.amrita.edu/?sub=3&brch=77&sim=218&cnt=1 and http://vlab.amrita.edu/?sub=3&brch=77&sim=885&cnt=1.
Answer the following questions 1. Explain the role of detergent or meat tenderizer and NACl. ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ _______________________________________________________________________ 2. Differentiate HOT SHOT METHOD from the conventional DNA extraction method (amritavlab). ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ _________________________________________________________________________ 3. Give one example of commercially available DNA kit and describe their contents and protocol. ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ _______________________________________________________________________
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