Maintenance Guide MR-96

Maintenance Guide
MR-96 Microplate Reader

CLINDIAG SYSTEMS B.V.B.A.
Contents
1 DESCRIPTION................................................................................................... 1
1.1 BASIC PRINCIPLE......................................................................................... 1
1.2 STRUCTURE CHART...................................................................................... 1
1.3 ASSEMBLY SCHEMATIC DIAGRAM................................................................... 2
2 OPERATIONAL ENVIRONMENT...................................................................... 2
3 WARNINGS AND PRECAUTIONS................................................................... 3
4 SERVICE AND MAINTENANCE...................................................................... 3
4.1 MAINTENANCE STAFF QUALIFICATIONS.......................................................... 3
4.2 TOOL............................................................................................................. 4
4.2.1 General Tools......................................................................................... 4
4.2.2 Specific Tools......................................................................................... 4
4.3 DAILY MAINTENANCE...................................................................................... 4
4.4 WEEKLY MAINTENANCE................................................................................ 5
4.5 MONTHLY MAINTENANCE................................................................................ 5
4.6 YEARLY MAINTENANCE................................................................................... 6
5 MAIN COMPONENTS...................................................................................... 6
5.1 MAIN CONTROL PANEL................................................................................... 6
5.1.1 Functions................................................................................................ 6
5.1.2 Structure Chart...................................................................................... 7
5.1.3 Circuit Diagram...................................................................................... 7
5.2 TEST BOARD................................................................................................... 7
5.2.1 Functions................................................................................................ 7
5.2.2 Structure Chart...................................................................................... 8
5.2.3 Circuit Diagram...................................................................................... 8
5.3 MOTOR DRIVEN BOARD................................................................................ 8
5.3.1 Functions................................................................................................ 8
5.3.2 Structure Chart...................................................................................... 8
5.3.3 Circuit Diagram...................................................................................... 8
5.4 OPTICAL SYSTEM......................................................................................... 9
5.4.1 Functions................................................................................................ 9
5.4.2 Structure Chart...................................................................................... 9
6 REPLACEMENTS......................................................................................... 10
6.1 REPLACEMENT OF LAMP............................................................................. 10
6.2 REPLACEMENT OF FILTER.......................................................................... 11
6.2 REPLACEMENT OF PRINTER...................................................................... 11
6.3 REPLACEMENT OF FUSE............................................................................. 12
7 TROUBLESHOOTING................................................................................... 13
7.1 TROUBLESHOOTING FOR COMMON PROBLEMS............................................. 13
7.2 Troubleshooting for Reagents................................................................... 15
1 Description
1.1 Basic Principle

Based on the Lambert-Beer law, microplate reader uses immune complex of
enzyme labeling and corresponding substrate from enzyme to produce color
reaction. Then it will be able to determine analyte concentration with different
absorption lines and absorption values.
1.2 Structure Chart

Structure chart of the instrument is shown as Figure1-1.
Light Filter Plate Detection Control Panel

Board
Motor Drive
Motor
Drive Panel LCD Screen
Filter Drive
Positioning Panel Control

Panel
12V Power 5V
Source
24V 15V
Figure 1-1 Structure chart
MG 02 17/08/2009 MR-96 Microplate Reader Page 1 of 20

1.3 Assembly Schematic Diagram
Assembly schematic diagram of the instrument (Wiring Schematic diagram) is
shown as Figure1-2.
Touch Screen
Indicator
Control Board
Inverter
Fan
Back Cover
Lamp
Motor
Test Board
Power Control
Motor Motor
Drive
Control
Positioning Board
Test Board
Figure 1-2 Schematic diagram
2 Operational Environment
Operational environment requires:
-- Normal operational temperature: 10℃ - 30℃
-- Relative humidity: 30% - 80%
-- Air pressure: 86KPa - 106 KPa
-- Avoid dust, noise or power interference.
-- Keep the instrument away from centrifugal, CT, X-ray apparatus and
interference sources from strong current, magnetic objects and shocks as well as
super power instruments, which require frequent switching on/off.
-- Avoid sunlight, direct ultraviolet light, heat sources, cold sources and air
outlets such as air conditioner.
Note
-- Check and confirm that all plugs on the instrument are properly connected,
well grounded and reliable.
-- Make sure that the instrument is placed on a smooth and stable table.
3 Warnings and Precautions

-- Do not operate the instrument near water sources or in the vicinity of fluid.
-- Do not place or operate the instrument on an unstable table.
-- Do not place the instrument close to radiators or heat sources.
-- There are ventilating openings at bottom panel and back panel. Make sure to
reserve a 20 cm-space at both sides for colling down. Also do not operate the
instrument in a closed environment.
-- Check and confirm that the power socket is correctly installed and well
grounded.
-- Check and confirm that all plugs on the instrument are properly connected,
well grounded and reliable.
-- Do not plug/unplug serial communication cable or other wires while the
instrument is running.
-- Make sure to use qualified reagents, control materials and standards that are
within periods of validity.
-- Do not make physical concact with patients’ serums to avoid potential risks.
-- Dispose waste samples and fluid in accordance with state and local laws or
regulations.
-- Make sure to use matched supply voltage on the instrument.
-- Be sure to cut off the power and turn off the instrument before repairing it.
Performing repairs with the instrument running may result in electric shock or
coponent breakage, and should be done by professional engineers.
-- Always use a specified fuse.
4 Service and Maintenance
4.1 Maintenance Staff Qualifications

Service is only provided by Clindiag after-sale engineers or our authorized
professionals.

4.2 Tools
4.2.1 General Tools

General tools for maintenance:
-- 1 medium-sized screwdriver
-- 1 small screwdriver
-- 1 medium-sized slot-head screwdriver
-- 1 small slot-head screwdriver
-- 1 pair needle nose pliers
-- 1 set inner hexagon spanner
-- 1 150W electric iron
-- 1 pack soldering tin
-- 1 digital multimeter
-- 1 pair of tweezers
4.2.2 Specific Tools

-- Soft cloth
-- Neutral cleaner
-- Lubricants
-- Cotton swap
-- Rubbing alcohol
-- Rubber gloves
-- Lens paper
4.3 Daily Maintenance

Daily maintenance steps are as follows:
-- Clean microplate reader’s operational environment.
-- Use a cloth with neutral cleaner to clean surface of the instrument.
-- Use a soft cloth to clean the screen.
Note
Do not allow any solvent, grease or corrosive substances to drop on the
instrument.
Warning
When cleaning with detergent, be sure to wear safe-guard glasses, rubber
gloves and strictly follow Warnings & Precautions on the bottle. If there is any
detergent dropped on your body, wash it with clean water and disinfectant
immediately. If necessary, go and see your doctor.
4.4 Weekly Maintenance

Weekly maintenance steps are as follows:
-- Use a soft cloth with neutral cleaner to clean surface of the instrument.
-- Use a soft cloth with neutral cleaner to clean slide rails of the instrument.
Note
Do not allow any solvent, grease or corrosive substance to drop on the
instrument.
Warning
-- Do not clean slide rails of the instrument with solution containing
hypochlorite. Failure to do so may result in permanent corrosion.
-- When cleaning with detergent, be sure to wear safe-guard glasses, rubber
4.5 Monthly Maintenance

Monthly maintenance steps are as follows:
-- Use a soft cloth with neutral cleaner to clean slide rails of the instrument.
-- Apply lubricants to guide rods of slide rails.
Note
instrument.
Warning
4.6 Yearly Maintenance

Yearly maintenance steps are as follows:
-- Open the upper cover and use a soft cloth with neutral cleaner to clean slide
rails of the instrument.
-- Open the upper cover and apply lubricants to guide rods of slide rails.
-- Open the upper cover and wipe the dust off the filter and lens with lens
paper.
Note
instrument.
Warning
-- Do not stain lens or filter when cleaning with reagents or solvents. Failure to
do so may result in abnormal test results.
5 Main Components
5.1 Main Control Panel
5.1.1 Functions
Microplate reader (Microplate reader for ELISA), which is composed of a main
control panel and a front panel, adopts a master-slave design mode. Once the
main control panel issues an instruction, the front panel will execute it and
complete such tasks as motor control, data processing & display, printing as well
as data storage & inquiry.
5.1.2 Structure Chart

Structure chart of main control panel is shown as Figure1-2.
5.1.3 Circuit Diagram

Circuit diagram of main control panel is shown as Figure5-1.

1 2 3 4
VCC
X1 U4 HY62WT08IED70C
1 2 U1 U2 A0 10 11 D0
12 P89C668HBA VCC 74HC373D A1 9 A0 D0 12 D1
VSS
44
1
12
23
34
22
CX1 CX2 A1 D1
NC
NC
NC
NC
A2 8 13 D2
20pF 22.1184MhZ 20pF GND 1 A3 7 A2 D2 15 D3
21 ALE 11 OC 2 A6 A4 6 A3 D3 16 D4
D 20 XTAL1 C 1Q 5 A4 A5 5 A4 D4 17 D5 D
CRY_X1 CRY_X2 XTAL2 36 D7 D6 3 2Q 6 A2 A6 4 A5 D5 18 D6
P0.7/AD7 37 D6 D4 4 1D 3Q 9 A0 A7 3 A6 D6 19 D7
10 P0.6/AD6 38 D5 D2 7 2D 4Q 12 A1 A8 25 A7 D7
VCC 35 RST P0.5/AD5 39 D4 D0 8 3D 5Q 15 A3 A9 24 A8
U8 MAX810 CRY_X1
PSEN 32 EA/VPP P0.4/AD4 40 D3 D1 13 4D 6Q 16 A5 A10 21 A9
2 CRY_X2 33 PSEN P0.3/AD3 41 D2 D3 14 5D 7Q 19 A7 A11 23 A10
VCC RESET ALE/PROG P0.2/AD2 42 D1 D5 17 6D 8Q A12 2 A11
3 P0.1/AD1 43 D0 D7 18 7D A13 26 A12
VCC RESET
/RD19 P0.0/AD0 8D A14 1 A13
1 /WR18 P3.7/RD 9 A14
GND FMQ17 P3.6/WR P1.7/SDA 8 U3 /RD 22
RESET ALEIRQ 16 P3.5/T1/CEX4 P1.6/SCL 7 74HC138D A15 20 OE
15 P3.4/T0/CEX3 P1.5/CEX2 6 A13 15 /WR 27 CE
P_BUSY
14 P3.3/INT1 P1.4/CEX1 5 A14 Y0 14 WE
TxD 13 P3.2/INT0 P1.3/CEX0 4 A15 Y1 13
RxD 11 P3.1/TxD P1.2/ECI 3SDA LED0 Y2 12
PSEN P3.0/RxD P1.1/T2EX 2SCL
P_DATA_STROBEVCC 6 Y3 11 CS3
1 P1.0/T2 DOUT GND 4 Y4 CS2 GND VCC
C 2 RA15 GND
4.7K
VCC
PEN DIN
31 GND1 5 A G2A Y5 10
9 C
VCC 1 2 CE_1
A15 + CE_2 P2.7/A15 CS_TCH
30 2 G2B Y6 7
P2.6/A14 DCLK 3 B Y7
29 C D0
JMP 10uF 104 P2.5/A13 28 A12 D1 U5 DS12887A
P2.4/A12 27 A11 D2 IRQ
P2.3/A11 G1
26 A10 D3 1 MOT VCC 24 /RST2
P2.2/A10 A15 A9
25 CS1 D4 2 23 /RD
P2.1/A9 A14 A8 NC SQW
24 D5 3 NC NC 22
VCC P2.0/A8 A13 D6 4 21 /WR
AD0 /RCLR
U10B 74HC00D U10A 74HC00D D7 5 AD1 NC 20 ALE
CE_11 CE_12 CE_13 CE_14 CE_15 CE_16 4 1 GND 6 19 CS2
7 AD2 /IRQ 18
RESET 6 /RST1 RESET 3 /RST2 AD3 /RST
104 104 104 104 104 104 5 2 8 17
9 AD4 DS 16
10 AD5 NC 15
11 AD6 R/W 14
12 AD7 AS 13R9_1
GND /CS 470
A K LED0
VCC VCC 12
B VCC B
CE_5 + CE_6 D0 LED
CU7_4
CU7_2 1uF 10uF 104
CZ2 1uF R9_2 470
A K GND
1
DB9 12
VCC R9_6
5 4.7K D1 LED F1
3
CE_3 CE_4
+
9 + R9_3
U7
+
4
10uF 104 4.7K T1
2
MAX232CSE FMQ 1
VCC
8 2 2 9013
2
6V+
16V-
3 OTXD GND VCC SPEAKER

7 13 R1 IN R1 OUT 12
2 ORXD 8 9 RxD VCC
1
6 11 R2 IN R2 OUT 14
1 TxD 10 T1 IN T1 OUT 7 U6 AT24LC256I/P
1 T2 IN T2 OUT 4
3 C1+ C2+ 5 1 8 VCC
R9_4VCC4.7K
+ CU7_1 C1 - C2 - CU7_3 + 2 VCCA0/WP 7 SCL
GND Title
GND
A1 TEST
A 1uF 1uF 3 6 SCL . A
15
GND 4 A3 SCL 5 SDA

VSS SDA
R9_5 4.7K Size Number Revision
VCC SDA A4
Date: 9 Sheet of
File: ¿° y:
1 2 3 4
Figure 5-1 Circuit diagram of main control board
5.2 Test board
5.2.1 Functions
Test board converts optical signals into electrical signals, which will be
processed by such analog signals as pre-amplification, gain control and analog-
to-digital conversion. Afterwards, the signals will be sent to front MCU for data
sampling and storage. Finally, the data will be sent from front end to back end
through serial communication and the processing results will be displayed on
LCD or printed by the printer.

Structure chart of test board is shown as Figure 1-2.

Circuit diagram of test board is shown as Figure 5-2.
1 2 3 4
HR2 CZ3 IDC10

A3144E VCC 1 M1_CLK
U11 74HC377D M1_EN
1
/A15 1 2 M2_EN
RHR1 /WR 11 G 2 VOLTAGE 3 M2_CLK
240 CLK 1Q 5 LED2 4 CW/CCW
D0 3 2Q 6 LED1 5 HALF/FULL
VCC2
GND3
OUTPUT
D D2 4 1D 3Q 9 LED0 6 GND D
1
2 D4 7 2D 4Q 12 HALF/FULL 7 N_PLATE_LOCATION
小车复位信号 D6 8 3D 5Q 15 CW/CCW 8 N_AD_SYNC
N_PLATE_RESET D7 13 4D 6Q 16 M2_EN 9 N_FILTER_RESET
VCC VCC D5 14 5D 7Q 19 M1_EN 10
D3 17 6D 8Q
D1 18 7D
8D
VCC
VSS
44
1
12
23
34
22
NC
NC
NC
NC
CX2 X1 CX1
20pF 2 1 20pF
JMP 21 X1 21
PSEN X2 20 XTAL1
1 GND 22.1184MhZ XTAL2 36 D7
2 U10 MAX810 P0.7/AD7 37 D6
2 RESET 10 P0.6/AD6 38 D5
VCC RESET VCC 35 RST P0.5/AD5 39 D4 U8F 74LS14
3 PSEN 32 EA/VPP P0.4/AD4 40 D3 FILTER_RESET 12 13 N_FILTER_RESET 滤光轮零位信号
VCC 33 PSEN P0.3/AD3 41 D2
1 ALE/PROG P0.2/AD2 42 D1
C GND P0.1/AD1 43 D0 C
RAGND 0 /RD 19 P0.0/AD0 RA15 U8B 74LS14
P3.7/RD 10K 小车定位信号
1 2 /WR 18 9 SDA VCC 1 2 A15 PLATE_LOCATION 4 3 N_PLATE_LOCATION
GND /M2_CLK 17 P3.6/WR P1.7/SDA 8 SCL
/M1_CLK 16 P3.5/T1/CEX4 P1.6/SCL 7 GAIN2
PLATE_LOCATION 15 P3.4/T0/CEX3 P1.5/CEX2 6 GAIN1
AGND AD_SYNC 14 P3.3/INT1 P1.4/CEX1 5 GAIN0 U8A 74LS14
TxD 13 P3.2/INT0 P1.3/CEX0 4 CHANNEL2 AD_SYNC 2 1 N_AD_SYNC 斩光电机同步信号
P3.1/TxD P1.2/ECI U9C
RxD 11 P3.0/RxD P1.1/T2EX 3 CHANNEL1 9
P1.0/T2 2 CHANNEL0 8 /A15
A14 10 U8C
CU6_1 CU7_1 CU8_1 CU9_1 CU10_1 CU11_1 CU12_1 P2.7/A15 31 A15
104 104 104 104 104 104 104 30 A14 A13 74LS00 PLATE_RESET6 5 N_PLATE_RESET 小车复位信号
VCC P2.6/A14 29 A13 1
P2.5/A13 28 3 AD_CS
P2.4/A12 74LS14
P2.3/A11 27 PLATE_RESET 2
26 FILTER_RESET U9A 74LS00
P2.2/A10 25 AD_OK U12 AT24C08N
P2.1/A9 24 AD_A0
B P2.0/A8 U9B 74LS00 B
VCC /WR 4 1 A0/WP VCC 8 VCC
6 AD_CE 2 A1 TEST 7 GND
U6 /RD 5 3 A3 SCL 6 SCL
CU7_3 P89C668HBA GND 4 5 SDA
1uF CU7_5 1uF VSS SDA
R9_1 470 A K LED0
VCC 12
U8D 74LS14
+
R9_2 470 D0 LED
LED1
+
CZ2 U7 A 12 K /M1_CLK 9 8 M1_CLK

VCC
3PIN/2.54 MAX232CSE
2
6V+
16V-
R9_3 470 D1 LED

3 OTXD 13 12 1 2A K LED2
2 ORXD 8 R1 IN R1 OUT 9 RxD 12 U8E 74LS14
1 11 R2 IN R2 OUT 14 D2 LED /M2_CLK 11 10 M2_CLK
TxD 10 T1 IN T1 OUT 7
1 T2 IN T2 OUT 4
3 C1+ C2+ 5
+ CU7_2 C1 - C2 - CU7_4 + VCC
R9_4 10K SCL Title
GND
A 1uF 1uF 1 A
15
R9_5 10K Size r Revision

VCC SDA A4
Date: 3-Jul-2009 Sheet of
File: Drawn By:
1 2 3 4
Figure 5-2 Circuit diagram of test board
5.3 Motor Driven Board
5.3.1 Functions
Motor driven board is to control motor rotation in micro plate and in filter
wheels so as to achieve reciprocating motion of micro plate and to switch filters.

Structure chart of motor driven board is shown as Figure1-2.

Circuit diagram of motor drive board is shown as Figure 5-3:

Figure 5-3 Circuit diagram of motor drive board
5.4 Optical System
5.4.1 Functions
Light from light sources goes through diaphragm and condenser to the
reflector and is reflected at 90 degree. Then through the filter, the reflected light
is transformed into monochromatic light. Condensed by optical fiber light-emitted
lens and transmitted by optical fiber, the monochromatic light then passes
through color solution vertically and focuses on test board.

Structure chart of optical system is shown as Figure 5-4.
Light Source
Beam Convex Reflector
Photocell Solution Filter Convex

Figure 5-4 Structure chart of optical system
6 Replacements
6.1 Replacement of Lamp

Make sure that the lamp doesn’t work or has worked for a total of 2,000 hours
before replacing. Lamp and lamp holder are shown below in Figure 6-1. Use the
following steps to replace them:
-- Switching off the instrument for 15 min. before opening the cover.
-- Unscrew the screws that secure shadow shield before opening it.
-- Pull out lamp holder together with the lamp, and then remove the lamp from
its holder.
-- Match the new lamp with the holder (OSRAM64607, 8V/50W) and reset it.
-- Close the shadow shield and tighten the screws.
-- Close the cover and restart the instrument.
Figure 6-1 lamp
Note
After replacement, check if the lamp connecting wire is properly connected.
Warning
-- Check and confirm the instrument is switched off when replacing lamp.
Replace lamp when the instrument is running may cause damage to new lamp.
-- Do not replace lamp immediately after the instrument is switched off. Failure
to do so may get you burnt by its extremely hot surface.
-- Do not touch the glass surface of the lamp. Failure to do so may result in
changes in the lamp’s characteristic. If there is any fingerprint on the glass, use a
cloth with rubbing alcohol to clean it.
6.2 Replacement of Filter
Schematic diagram of filter is shown as Figure 6-2. Use the following steps to
replace it if necessary:
-- Locate the filter needs to be replaced with the help of filter Schematic
diagram.
-- Remove snap rings from the filter with tweezers.
-- Remove the faulty filter.
-- Position the new filter to the original position and tighten it with snap rings.
Figure 6-2 Filter

Note
-- After filter replacement, check and confirm it is tightened by snap rings.
Failure to do so may result in abnormal noises when the instrument is started.
-- If there are any fingerprints or stains on the filter, use lens paper to clean it.
-- Note direction of assembly when replacing.
6.2 Replacement of Printer

Use the following steps to replace printer when it is necessary:
-- Switch off the instrument before opening the upper cover.
-- Unplug power cord and data cable of the printer.
-- Unscrew the screws that secure the printer.
-- Remove the broken printer.
-- Replace it with a new printer and set the dial plate according to Figure 6-3.
-- Connect power cord and data cable.
-- Secure the printer on the cover.

Figure 6-3 Printer
6.3 Replacement of Fuse

Use the following steps to replace fuse if necessary:
-- Turn off the instrument.
-- The fuse is installed in a fuse box close to the power switch at the back of
the instrument. Uncover the box lid and replace with a new fuse of the same
specifications.
-- Snap down the lid and restart the instrument.
Note: Always use a specified fuse.
7 Troubleshooting
7.1 Troubleshooting for Common Problems
The guide to solve common problems is shown as table7-1.
Table 7-1 Troubleshooting for common problems

Problem Cause Solution
The instrument Abnormal power 1 Check if the instrument is
fails to start supply switched on.
2 Check the power plug for
any loose.
Blown fuse Replace the fuse.
Improperly plugged Check power supply

power line voltage.
No signal after Power inverter on the Replace power inverter or

being started, but main board does not the main board.
instrument can work.
perform self Faulty display screen Replace the display
check. screen.
Lamp doesn’t Abnormal power Check voltage on the lamp.
work supply
Lamp failure Replace the lamp.
Signal value of a Failure lamp Replace the lamp.
certain Aged lamp Adjust the diaphragm
wavelength too horizontally or the reflector
low after self- vertically.
check.
No reset signal on Sampler tilts too Push the sampler to the
microplate much to the right. left by 1 cm.
Damaged horizontal Replace the horizontal
motor. motor.
optical coupler. optical coupler.
Sampler doesn’t Snap-gauge is not Replace the snap-gauge.
move placed into microplate
properly.
Foreign matters found Remove foreign matters
in sampler from sampler.
Loose or broken belt Tighten the belt or replace
it with a new one.

motor motor.
Printer fails to Printer abnormal Check the power plug for

start power supply any loose.
Printer dial plate is Check if the ON button is
not set correctly. pressed.
Built-in printer Printer power line is Reconnect printer power
doesn’t work not properly line.
connected.
Printing head is not Check and confirm the
reset. printing head is reset.
Printer data cable is Reconnect printing cable.
not properly
connected with the
main board.
Printing paper Correctly load with printing
incorrectly loaded paper.
Broken printer Replace the printer.
Incorrect printer Check and confirm the
settings printer is set correctly.
Poor print quality Replace the cartridge or
with external clean the printing head
printer Details please refer to
User’s Manual of Printer).
Paper jam in Details please refer to
external printer User’s Manual of Printer.
Failure of external Check the connections
printer between printer and main
boards as well as
operating requirements for
the printer Details please
refer to User’s Manual of
Printer).
Note
-- Only components provided by Clindiag or its distributors are allowed for
replacement.
-- If any unsolvable or repetitive errors arise, please contact us or our
distributor.

7.2 Troubleshooting for Reagents
Troubleshooting for reagents is listed as Table 7-2.
Table7- 2 Troubleshooting for reagents
Problem Cause Solution
light color and low Reagent kit is not Store reagent kit
sensitivity properly stored. according to its
instruction.
Reagent kit expires. Replace with a

qualified reagent
kit.
Reagent or sample is Allow reagent and
not balanced before samples to stabilize
use. at room
temperature for 10
min. before use.
Poor measurement Calibrate the
accuracy of pipettor, pipettor until it
dirty suction nose or coincides with
too much water in suction nozzle
suction nose closely. Make sure
to pipette slowly
and always use a
disposable suction
nose.
Temperature in Check and adjust
incubator is below temperature in
37℃; incubator when the
reaction plate is put
in. Do not open the
incubator frequently
during incubation
period so as to
avoid temperature
changes.
Insufficient incubating Use a clock for
time precise timing.

Excessive impact Reduce impact
force from rinsing, force and control
soaking time in the soaking time &
cleaner or rinsing rinsing frequency in
frequency accordance with
the instruction.
Insufficient When adding
chromogenic agents, chromogenic
chromogenic agents agents, the bottle
added in reverse order should be held in a
or add mixed vertical down
chromogenic agents; position and add
with uniform
strength at a
moderate speed.
Add chromogenic
agent A first then B
and never mix them
together.
Insufficient time for Use a clock for
substrate precise timing.
Poor quality distilled Determine the
water influence on
enzyme
immunoassay by
adding distilled
water into reagents.
Use NaN3 as Never add NaN3
corrosion prevention into samples.
of the samples, which
inhibits enzyme
reaction.
Poor Repeatability Different number of When repeating
samples or different tests of a certain
sampling time. sample, make sure
sampling time is
close to that of the
first sampling.
Samples not mixed Mix samples in the
well mixer after adding
samples.
Wrong filter or Use wavelength of
incorrect wavelength. 450/630 for TMB.

Poor repeatability of Recalibrate the
microplate reader. microplate reader.
Incorrect rinsing Add adequate
method. cleaning solvent
into each hole on
the plate and rinse
it with reaction
plate facing
downward; Shake
off residues on the
plate and pat dry it
on a clean or dust
free water
absorbent
materials; Make
sure holes are not
blocked and the
plate is thoroughly
washed.
Different incubation When using

conditions (Incubator incubator for
and water incubation testing, the color is
are used respectively darker and the OD
for the two tests.) value is 0.1-0.15
higher compared
with that of water
incubation. It is
recommended that
an incubator should
be used for both
tests. If water
incubation is
adopted, make
sure water
temperature is
37℃.
Accidentally add more More enzyme
or less enzyme than causes dark color,
needed. while less enzyme
leads to light color;
Note with a marker
pen for further
analysis.

Different sample Make sure the
amount retest is done by
Inconsistent the same operator
incubation time with the same
Different rinsing pipette and suction
methods (conditions) nose under the
same conditions,
avoiding any
possibility of
inconsistent results.
Not chromogenic for Poor quality distilled Compare it with
positive reference water. qualified distilled
water.
Incorrect method of Prepare cleaning
preparing cleaning solvent in line with
solvent. instructions.
Mistake stop solution Read the label
for cleaning solvent. carefully before
Mistake stop solution preparing cleaning
for substrate buffer. solvent.
Enzyme missing. Do not forget to
add enzyme.
Chromogenic agent A Add some
or B missing. chromogenic agent
and observe the
liquid level.
Inactivated enzyme Use vessels in the
remains in a certain reagent kit. Or use
vessel. clean and reliable
vessels.
All are chromogenic Poor water quality. Prevent distilled

water from being
contaminated.
Add too much enzyme Check if regulation
( Mistake 100uL for of the pipettor is at
50uL) or add too much a proper standard
ALT when diluting. before adding
enzyme; Focus you
attention on diluting
enzyme.

Excessive Check incubator
temperature in temperature before
incubator (exceeds setting the board
37℃), excessive and calculate the
reaction time of exact time.
enzyme conjugates, or
excessive color
developing time of
substrate.
Residue or enzyme Thoroughly rinse
remains in samples. the plate.
Prolonged substrate Prepare substrate
preparation or after enzyme
contaminated reaction (5 min.
substrate. before staring
rinsing).
Samples are Samples should be
contaminated for too kept at low
much exposure to temperatures and
outer. avoid being
contaminated.
Reuse pipette tip, Use a disposable
which had not been pipette tip.
cleaned or sterilized
properly, to add
enzyme or substrate.
Changes in plate Careless operation Strictly follow the
color instructions to rinse
plate, add samples
and develop color.
Plate rinsing is of
great significance.
Microplate washer is Check and adjust
blocked, too much microplate washer.
residues on the plate,
or cleaning solvent is
not added or aspirated
unevenly.
Improper centrifuging Proper centrifuging
treatment of samples, treatment of 3000
clotting or cellular rpm for 6 min.
constituents found in
reaction well.
Chromogenic Too many samples, Adopt multi-

sequence is not prolonged sampling channel constant
even. time or inconsistent reagent shifter to
reaction time. reduce sampling
time or control
quantity of each lot.
Use multi-stepper
to reduce sampling
time or control
sample amount.
Note
-- Follow reagent kit instructions to use. Failure to do so may result in uncertain
test results.
-- Mixture of reagents from different lots (standard A or enzyme working
solution), or mixture of different reagents of the same lot may result in light
color.
-- If whole white or whole chromogenic appears on reaction plate, a laboratory
step may be wrong. Check each step and if the problem remains, refer to
reagent of other lot to make sure reagent is qualified.
-- If there are any unsolvable or repetitive errors during operation, please
contact us or our distributors.
END

CLINDIAG SYSTEMS B.V.B.A.

Maintenance Guide MR-96

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Maintenance Guide

MR-96 Microplate Reader

1.1 Basic Principle

1.2 Structure Chart

Light Filter Plate Detection Control Panel

Positioning Panel Control

Figure 1-1 Structure chart

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Figure 1-2 Schematic diagram

3 Warnings and Precautions

4 Service and Maintenance

4.1 Maintenance Staff Qualifications

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4.2.1 General Tools

4.2.2 Specific Tools

4.3 Daily Maintenance

4.4 Weekly Maintenance

4.5 Monthly Maintenance

4.6 Yearly Maintenance

5.1 Main Control Panel

5.1.2 Structure Chart

5.1.3 Circuit Diagram

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3 OTXD GND VCC SPEAKER

GND 4 A3 SCL 5 SDA

Figure 5-1 Circuit diagram of main control board

5.2 Test board

5.2.2 Structure Chart

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HR2 CZ3 IDC10

CZ2 U7 A 12 K /M1_CLK 9 8 M1_CLK

R9_3 470 D1 LED

R9_5 10K Size r Revision

Figure 5-2 Circuit diagram of test board

5.3 Motor Driven Board

5.3.2 Structure Chart

5.3.3 Circuit Diagram

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5.4 Optical System

5.4.2 Structure Chart

Beam Convex Reflector

Photocell Solution Filter Convex

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6.1 Replacement of Lamp

Figure 6-1 lamp

Figure 6-2 Filter

6.2 Replacement of Printer

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6.3 Replacement of Fuse

Table 7-1 Troubleshooting for common problems

Improperly plugged Check power supply

No signal after Power inverter on the Replace power inverter or

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Printer fails to Printer abnormal Check the power plug for

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Reagent kit expires. Replace with a

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Different incubation When using