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    T47D Whole Cell Lysate Ab14899 T47D Whole Cell Lysate Ab14899

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    Rahmailla Khanza Diana Febriliantri
    T47D whole cell lysate is extracted from T47D breast cancer cells. [1] It is supplied as a liquid in a storage buffer and should be aliquoted and stored at -80°C to maintain stability. [2] T47D cells are used as a model for studying drug resistance in breast cancer as they express mutant p53 and can lose estrogen receptor expression with long-term estrogen deprivation. [3]

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    T47D Whole Cell Lysate Ab14899 T47D Whole Cell Lysate Ab14899

    Uploaded by

    Rahmailla Khanza Diana Febriliantri
    22 views2 pages
    T47D whole cell lysate is extracted from T47D breast cancer cells. [1] It is supplied as a liquid in a storage buffer and should be aliquoted and stored at -80°C to maintain stability. [2] T47D cells are used as a model for studying drug resistance in breast cancer as they express mutant p53 and can lose estrogen receptor expression with long-term estrogen deprivation. [3]

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    t47d whole cell

    Original Title

    datasheet_14899

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    T47D whole cell lysate is extracted from T47D breast cancer cells. [1] It is supplied as a liquid in a storage buffer and should be aliquoted and stored at -80°C to maintain stability. [2] T47D cells are used as a model for studying drug resistance in breast cancer as they express mutant p53 and can lose estrogen receptor expression with long-term estrogen deprivation. [3]

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    22 views2 pages

    T47D Whole Cell Lysate Ab14899 T47D Whole Cell Lysate Ab14899

    Uploaded by

    Rahmailla Khanza Diana Febriliantri
    T47D whole cell lysate is extracted from T47D breast cancer cells. [1] It is supplied as a liquid in a storage buffer and should be aliquoted and stored at -80°C to maintain stability. [2] T47D cells are used as a model for studying drug resistance in breast cancer as they express mutant p53 and can lose estrogen receptor expression with long-term estrogen deprivation. [3]

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Product datasheet

    T47D whole cell lysate ab14899


    1 References

    Overview

    Product name T47D whole cell lysate


    General notes Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift
    Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use
    fractions and then storing them at -80ºC.

    Properties

    Form Liquid
    Storage instructions Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
    Storage buffer pH: 7.90
    Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20%
    Glycerol, 0.009% PMSF, 0.008% DTT
    Purity Whole Cell Lysate
    Background T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor
    suppressor protein p53 protein. Under normal culturing conditions, they express progesterone
    receptor constitutively and are responsive to estrogen. They are able to lose the estrogen
    receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model
    for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Our Abpromise to you: Quality guaranteed and expert technical support

    Replacement or refund for products not performing as stated on the datasheet


    Valid for 12 months from date of delivery
    Response to your inquiry within 24 hours

    We provide support in Chinese, English, French, German, Japanese and Spanish


    Extensive multi-media technical resources to help you
    We investigate all quality concerns to ensure our products perform to the highest standards

    If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
    please visit https://www.abcam.com/abpromise or contact our technical team.

    1
    Terms and conditions

    Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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