Declaration: Pamocea Insularum's Slime" Nur Diyana Binti Hamdan, Matric
Declaration: Pamocea Insularum's Slime" Nur Diyana Binti Hamdan, Matric
Declaration: Pamocea Insularum's Slime" Nur Diyana Binti Hamdan, Matric
I hereby declare that the work embodied in this report is the result of the original research
and has not been submitted for a higher degree to any universities or institutions.
______________________
Student
Name:
Date:
I certify that the report of this final year project entitled “Deternination Of Antioxidant And
Total Protein Content of Pamocea Insularum’s Slime” Nur Diyana Binti Hamdan, matric
number F14A0231 has been examined and all the correction recommended by examiners
have been done for the degree of Bachelor of Applied Science (Agriculture Technology) with
Honours,
Approved by:
___________________
Supervisor
Name:
Date:
i
ACKNOWLEDGEMENT
Assalamualaikum and have a good day. First of all I would like to express my sincere
gratitude to Allah S.W.T the Almighty because I was gave changes and His immeasurable
who gave me guidance, support and encouragement to make this research possible. Thank
you with all the knowledge and laboratory skills that you have teaches me. He helped me
from initial until final stage of this research and guide me how to runs and set up the
experiments.
and Mrs. Sha who always give me the advices and right way to use instruments in the
laboratory. Some of also have to stay after their work shift to let me finish my research.
Also, my greatest unlimited appreciation goes to my parents, En. Hamdan bin Harun
and Mrs. Che Jarah binti Che Mat because of their unlimited support and encouragement to
complete this research. They are the one makes me felt never give up in my learning
process at UMK.
I also would like to express my thankfulness all my SBP’s friends for their best out of
the best co-operations when I in need, inspirations and support to complete this research.
They spend time a lot if I need some explanations and confuse my research even late night.
They saw all my ups and down during research until I finish my final year project.
ii
Abstract
Pamocea insularum commonly known for bring negatives impact on aquatic ecosystems
especially for agricultural industry. Hence, instead of using pesticides to get rid of this
undiscovered value based product, why not it is used as a source of income and additional
revenue source to the farmers. This report is included the extraction method to get the slime
from the Pamocea insularum snail and determination of its properties content in the slime.
The properties that will be checked in the snail’s slime is antioxidant activity by using 1,1-
Dyphenyl-2-picryl-hydrazyl (DPPH) and total protein content using Bradford assay in
Pamocea insularum.
iii
Abstrak
Pamocea insularum dikenali dengan sebagai haiwan yang membawa impak negative
terhadap ekosistem akuatik terutamanya terhadap industri agrikultur. Oleh itu, daripada
menggunakan racun perosak untuk menghapuskan produk nilai yang belum diterokai, tidak
salah sekiranya ia digunakan sebagai sumber pendapatan dan sumber nilai sumber
tambahan kepada aetani yang menanggung kerugian. Laporan ini akan membincangkan
tentang pengekstrakan untuk mendapatkan lendir daripada siput Pamocea insularum dan
penentuan sifat kandungan di dalam lendir. Sifatnya akan ditentukan adalah antioksidan
aktiviti menggunakan 1,1-Dyphenyl-2-picrylhydrazyl (DPPH) dan keseluruhan kandungan
protein mnggunakan Bradford assei dalam Pamocea insularum.
iv
TABLES OF CONTENT:
PAGE
DECLARATION i
ACKNOWLEDGEMENT ii
ABSTRACT iii
ABSTRAK iv
TABLE OF CONTENTS v
LIST OF TABLES vi
LIST OF FIGURES
CHAPTER 1 INTRODUCTION 1
1.3 Objectives 3
2.3.1 Plants 8
2..5 Microoragnisms 10
2.4.1 Morphology 12
2.4.2 Habitat 13
v
2.4.3 Feeding, Growth and Reproduction 14
CHAPTER 3 METHODOLOGY
3.2 Apparatus 16
3.3 Methods 16
CHAPTER 4 RESULTS
Activity
vi
LIST OF GRAPH
No. PAGE
vii