BSC 15iia2 X - Opr PDF
BSC 15iia2 X - Opr PDF
BSC 15iia2 X - Opr PDF
Class II A2
BSC-11IIA2-X
BSC-13IIA2-X
BSC-15IIA2-X
USER MANUAL
Thank you very much for purchasing our class II A2 biological safety
cabinet.
Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 [email protected]
MRC.10.16
CONTENT
1. Unpacking, Installation, Debugging ..................................................................................................3
1.4 Installation........................................................................................................................................8
2.1 Functions........................................................................................................................................14
4. Warranty ..........................................................................................................................................43
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1. Unpacking, Installation, Debugging
Please firstly check if packing box is in good condition. If the packing box is damaged, please take
photos.
1.1 Unpacking
Choose the proper unpacking method according to the actual situation.
1) Method 1 Necessary tools for unpacking: Electric drill with hexagon dead M8
Picture 1
3
Picture 2
Rapid unpacking diagram (Picture 3). Disassemble the screws shown in the below Picture, then
move the wooden pieces to right and left.
Picture 3
4
For carton box:
Using ordinary scissors to cut packing tape, take off the package cover, then move up the paper box
body.
Picture 4
5
1.2 Accessories checking
Refer to the packing list and check the accessories.
Packing list (BSC‐11/13/15IIA2‐X)
Items Model Position Quantity
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1.3 Installation conditions and using environment
To avoid disturbances to the safety cabinet and its operator, follow the following guidelines, while
determining a suitable location for the cabinet:
a. The distance from the plane of the aperture to any circulation space should be at least 1000 mm,
so as to preserve a zone undisturbed by anyone other than the operator.
b. Biological safety cabinets should be placed in a position where there should be no opposing wall
(or other obstruction likely to affect the airflow) within 2000 mm of the front aperture.
c. Safety cabinets should not be installed in positions where they are likely to be affected by other
items or equipment. In particular the distance to the aperture of an opposing safety cabinet, fume
cupboard, or the edge of a local exhaust ventilation outlet should not be less than 3000 mm.
d. Any room air supply diffuser should not be within 1500 mm of the front aperture.
e. Doorways should not be within 1500 mm of the aperture or within 1000 mm of the side of the
safety cabinet.
f. The position of a safety cabinet should satisfy the spatial requirements (e.g. vision, lighting and
convenience of access) of the operator and personnel working nearby. When a cabinet is installed
on a bench top, the leading edge should be flush with or slightly overhanging the edge of the
bench top.
Working environment:
(5) Electrical parameters: Consistent with the rated voltage of the biosafety cabinet (See 2.1.5
technical parameter performance index);
(6) Power supply need to be grounded; (Judging method: testing the fire wire and the zero line of the
power supply with multimeter, the fire wire to ground voltage should be grid voltage and the zero
line to ground voltage should be 0, otherwise the power supply ground is bad).
(7) Test the voltage stability before using, if the voltage is unstable, should use the voltage regulator,
otherwise the control panel and transformer may be easily damaged.
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1.4 Installation
a. Remove all the package materials;
b. Inspect the surface of main body to make sure whether there is scratch, deformation or
uncorrelated things;
The base stand will be packed at back of main body, please take it out before installation. DO NOT
Note: The base mounting bolts are fixed to the base stand. When install the base stand, except the 4
bolts marked with red circle in Picture 5, please remove the other bolts and install.
Remove the M10×55 Hexagon socket head screws, Buckle plug, M10 Stainless steel flat washer, M10
Stainless steel spring washer, M10 Stainless steel cap nuts, referring to Picture 5 assembled base,
firmly fastening requirements.
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e. Connect base stand and main body
Picture 6
Aligned the mounting holes on the bottom of the cabinet side with the locating bolts, slow down the
cabinet on the mounting base.
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Picture 7
Take out the M10*55 Hexagon socket head screws, Flat washer 10, Spring washer 10 from the
accessory box, and fasten tightly according to Picture 7.
10
Picture 1
6. Drain valve
Take out drain valve coupling, shim, Ball nut, Rubber gasket, Drain valve, assembling from up to
down as Picture 8 illustrated.
Picture 2
Clockwise rotate caster’ red part to low down the base feet and the height of the cabinet. Low down
all four casters can move the cabinet position. Counterclockwise rotate casters’ red part can rise the
base leg and height of cabinet. Raise all four casters can at same time can fix the cabinet. Adjust the
four Foot -masters makes the cabinet stable.
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h. Foot Switch
Picture 3
Install Foot Switch as Picture 10. It’s socket is at the left top, connect the plug.
Picture 4
1. Fastening Nut
Take out fastening nuts , water and gas taps, installing as Picture 11.
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1.5 Checking after installation
First, make sure the Voltage and frequency to be same as logo showing, and then check the follows
items with power on:
Foot Switch Red pedal for glass up, black pedal for glass down
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2. User Instructions
2.1 Functions
2.1.1 Product Concept
This products belong to Class II A2 biological safety cabinet which fully meet the requirement of US
standard ANSI/NSF49:2002, European standard EN12469:2000, biological safety cabinet is a kind
of negative pressure filtration system for protecting operator, the laboratory environment and work
materials, the front opening which air flow inward have protection function for operator, the filtered
laminar flow generated by vertical HEPA can protect work materials, what’s more, the polluted air
flow become pure after processed by HEPA(ULPA) filter. When it’s used in microbiology
experiment environment filled with volatile or toxic chemical and radionuclide, suitable exhaust
hood in function have to be linked.
Biological Safety Cabinet is necessary equipment in the laboratory in the search of microbiology,
biomedical, DNA recombinant, animal experiment, and biological products, especially in the
occasion that operator need to adopt protective measure, such as medical and health, pharmacy,
medical research. Our equipment provides a safety working environment which don’t have bacterial
and dust in the process of bacterial culture.
Picture 5
2.1.4 Protected objects
Biological safety cabinets (BSCs) are designed to protect the operator, the laboratory environment
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and work materials from exposure to infectious aerosols and splashes that may be generated when
manipulating materials containing infectious agents, such as primary cultures, stocks and diagnostic
specimens.
Model
BSC-11IIA2-X BSC-13IIA2-X BSC-15IIA2-X
Parameters
Frequency 50 Hz 60Hz
Working Zone
940×600×660 mm 1150×600×660 mm 1350×600×660 mm
Size(W*D*H)
Fluorescent lamp
21W*2 21W*2 28W*2
Consumption
Notes: (1) Electric consumption power including power which operation area needs to load (Loading
no more than 500W)
(2) Our company has right for changing the products, if we need to change and re-design,
please forgive us for not notifying you.
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2.1.6 Performance Index
2) Leak-proof Cabinet
If cabinet pressurized to 500Pa, the pressure should be no less than 450 Pa after 30 min.
Scan and detect the HEPA filter, the leakage rate at any point should not be >0.01%.
4) Vibration amplitude
The net vibration amplitude between frequency 10Hz and 10KHz is no more than 5μm(rms).
5) Illumination
The average illumination is no less than 650 lux, max illumination is no less than 1000lux.
6) Mechanical performance
Structure design is reasonable, high quality materials are adopted for the cabinet.
The working surface will not occur permanent deformation when weight put reaching 23kg.
7) Electrical properties
The voltage increases to 1390V(AC) in 5s and keep for another 5s without breakdown.
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2.2 Product structure
2.2.1 Structural composition of BSC-11IIA2-X
Picture 6
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2.2.2 Structural composition of BSC-13IIA2-X
Picture 7
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2.2.3 Structural composition of BSC-15IIA2-X
Picture 15
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Air Filtration System is the most important system of BSC. It consists of blower, supply filter and
exhaust filter. The function of Air Filtration System is transferring filtered air to work area, ensure
the down flow velocity, and keep Class 100 cleanness of work area.
3) UV Light
UV lamp is inside work area. So UV lamp can well sterilize all space of work area. Emission of
253.7 nanometers can ensure most efficient decontamination.
4) Fluorescent Light
The BSC is equipped with straight tube type energy-saving fluorescent lamp. It can make sure
average illumination inside work area which meets standard requirements.
5) Air pipe
Air pipe is the ventage of differential pressure sensor.
The air pipe should not be blocked and please do not hang anything on the pipes,
6) Power lock
When the power cord is connected to main power, switch the key for power lock, then the equipment
is powered on.
(1) Please make sure the total load of sockets should be ≤ 500W;
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The equipment is equipped with main power fuse, waterproof socket fuse and fan fuse (only for
BSC-15IIA2-X). They are located near the power cord’s outlet. Fuse label is corresponding to the
relevant specifications. Please refer to 3.2.
Picture 8
Large LCD display indicates detailed key parameters, it is real-time display to reflect the equipment
working condition, such as effective working state of the filter, which is more intuitive. (Please refer
to 2.3)
10) Structure
a) Biological Safety Cabinet’s both sides and back area are negative pressure air channel. And
the negative pressure keeps work area away from contamination.
b) Cabinet body is built of 1.2mm cold-rolled steel with anti-powder coating. Strong and steady.
c) Work area is fully made of 304 stainless steel which looks beautiful and with corrosion
resistance performance.
d) Base stand is made of cold-rolled steel with anti-powder coating.
e) Soft touch type control panel, easy to handle and beautiful appearance.
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2.3 Control panel
Picture 9
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a) LCD Screen
The working status of the equipment and operation can be seen on the LCD screen.
: To control blower working status. (It will not work when front window is fully
closed.)
Clock Adjustment:
Press the light button, and then press the power button for 5 seconds. Then you see the state of clock
adjustment after a buzzer alarm.
Firstly, minute position is flashing, press UP and DOWN to adjust to present time. Then press the
MUTE button switching to hour position and adjust to present time. After that, press the light button
first, and press the power button for about 5 seconds. Data will be saved after a buzzer alarm.
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2.4 Remote Control &Foot switch
This remote control adopt specific chip which is featured with good anti-jamming performance,
longer control distance and high control precision.
1. Power (POWER)
2. Reservation Time (SUB)
3. Timer (INSTALL TIMER)
4. Confirm (CONFIRM)
5. Cancel (CANCEL)
6. Turn up (+)
7. Turn down (-)
8. Fan (FAN)
9. UV (UV)
10. Illumination (LIGHT)
11. Socket (SOCKET)
12. Mute (MUTE)
13. Front window up (UP)
14. Front window down (DOWN)
Picture 10
Remote Control
Picture 11
Press the left red switch by foot, front window goes up, press the right black switch, front window
goes down.
(1) Make sure input voltage is correct and stable. The rated load of main power socket
should be higher than cabinet consumption. Plug must be well grounded.
(2) In order to avoid air turbulence, the operator should slightly move his arms during
experiment. Hands should stay inside the working area at least 1 minute before
operating. In order to decrease the times of arms moving into and out of the working
area, prepare all the necessary items inside the cabinet before starting experiment;
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(3) Moving principles of different samples inside cabinet: When two or more samples need
to be moved, be sure that low-polluting samples move to high-polluting samples.
Movement of items should also follow the principles of slow-moving.
(4) Samples placed in parallel: Samples should be placed in the cabinet parallel to avoid
cross-contamination between samples and blocking back air grille.
(5) In order to avoid samples being sucked into the negative passage or the blower, do not
place soft and slight samples (for example: soft tissue) on the surface during
experiment;
(6) The weight of items placed in the cabinet should be no more than 23Kg/25×25cm2;
(7) Avoid vibration: avoid using vibration equipment (eg centrifuges, vortex oscillator, etc.)
inside the cabinet. Vibration would cause lower cleanliness of operating area and affect
operator protection.
(8) No flame: No flame is allowed inside the cabinet. Using of fire will lead to airflow
disorder, and filter damage. If sterilization is required during the experiment, infrared
sterilizer is highly recommended.
(9) HEPA filter life: With the usage time increasing, dust and bacteria accumulate inside
HEPA filter. Filter Resistance is getting bigger, when it reaches the maximum point,
there will be audible and visual alarm. Please replace new HEPA filter, otherwise it will
affect the safety performance of the equipment. The used filter should be processed as
medical waste.
(10) There is a negative passage surrounding the work area, which is sealed strictly in the
factory. The operator is not allowed to remove or loose screws of those parts. If
necessary, please contact service personal.
(11) Front Grille is used for air intake and drain. Do not block it, otherwise it will affect
airflow. Armrest is recommended to solve this problem and reducing the operator's
wrist fatigue.
(12) Long-term use of biological safety cabinets will inevitably cause pollution (e.g. HEPA
filters, corner cabinets, etc.). In order to sterilize thoroughly every 500 hours, formalin
(formaldehyde) fumigation sterilizer is recommended. After sterilization, neutralize
formaldehyde gas with ammonium hydrogen carbonate. Make sure no sterilization gas
escapes during the whole process.
(13) The maximum storage period is one year. If the period is more than one year,
performance test should be done .
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Serious declaration: we will take no responsibility for risks caused by improper
b. Open the power lock, LCD display lights up and alarm rings at the same time, then the machine
enters to standby status. Waiting for the operator to input button to use it.
c. Press POWER button, then the following functions are available: Fluorescent lamp. UV lamp, Fan,
Mute, Sockets, Front window up and down, Reservation timing
When front window is closed and fluorescent light is off, then press the UV button to
d. Before doing experiment, please sterilize the cabinet for more than 30 minutes by UV lamp.
(1) For safety of eyes and skin, people should leave room during the UV sterilization.
(2)UV lamp intensity should be tested regularly. If there is no test conditions, it should be
replace when the UV timer on the display indicate the working time reaches to 1000 hours.
e. Please move the front window at 200mm height from the work table, turn on the fan, make sure
the experiment should be started after fan working for at least half an hour.
For operating safety, please put testing materials inside the cabinet in advance, and keep
the front window at 200mm height from the work table during operation.
After finishing the experiment, please move the front window down to the bottom, and make sure to
sterilize the cabinet by UV lamp for 30 minutes before turning off the cabinet.
2 . 6 . D a i l y m a i n t e nan c e
Because the operating time will directly affect the judgment of maintenance needs, we recommend
the user keep a detailed record of operating time for reference.
When doing maintenance, please pay attention to cut off the power, so as to avoid electric
shock!
Soap, hot water or warm water, a soft cotton cloth, dry cloth or towel, medical alcohol or other
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disinfectants, 100 dilution of household bleach, abrasive household cleaners, sterile water
Wipe the entire surface with a soft cotton cloth or towel soaked with concentrated liquid soap, then
wipe up the soap with another cotton cloth or towel soaked with clean hot or warm water, and then
wipe the surface with a dry cotton cloth or towel rapidly.
For the contaminated or dirty work surface or sump., use 70% medical alcohol or other disinfectant
to wipe.
Disinfectants used for wiping should not damage 304 stainless steel.
Use soft cotton cloth or towel to wipe the surface with non-abrasive household cleanser.
a. Check the two conveyor belts of front window drive unit, and ensure that their tightness is
coincident.
b. Check the UV lamp and fluorescent lamps.
c. Apply for testing the overall performance of cabinet on an annual basis to ensure the
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performance safety. User is responsible for testing costs.
d. Record this maintenance result.
2.6.5 Storage conditions
Safety cabinet should be stored in a relative humidity no more than 75%, the temperature is below
40℃, in the warehouse with good ventilation performance, no acid, no alkali and no other corrosive
gases, storage period shall not exceed one year, safety cabinet for more than a year needs to
unpacked and checked. Only the tested and qualified safety cabinet can be sold.
Disinfection is necessary when any contaminated part of the biosafety cabinet needed for routine
maintenance, replacement filters, and performance testing, etc. Before doing certification test and gas
sterilization, all internal working surface and the exposed outer surface should be disinfected with a
suitable disinfectant.
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2.8 Replacement parts list
AAQ02 Fuse 5A
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BSC-13IIA2-X Replacement parts list
AAP02 Fuse 5A
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BSC-15IIA2-X Replacement parts list
No Designation Standard
AAJ02 Fuse 5A
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1 2 3 4 5 6 7 8
D T&H SENSOR D
FOOT SWITCH UP
WINDOW H
WINDOW L
BLACK(Φ1)
UV BA LLAS T
BLACK(Φ0.5)
WHITE(Φ0.5)
UV LAMP WHITE(Φ0.5)
BLUE(Φ1)
RED(Φ0.5)
RED(Φ0.5)
WHITE(Φ0.5)
BLACK(Φ0.5)
BLUE(Φ0.5)
1 3
2
Window
H
L
GND
VCC
H T UV Ctrl
GND
BLUE(Φ0.5)
RED(Φ0.5)
up
Foot
YELLOW(Φ0.5) down
C BLACK(Φ0.5) C
LAMP YELLOW(Φ0.5)
220V/50Hz
ANJIAN
1 MCU EIGHT CORE CABLE
LCD
LIGHT BALLAST
2
RED(Φ0.5) GREEN(Φ1)
3
RED(Φ0.5) 4
TRANS-IN
5 BLUE(Φ1)
BLACK(Φ0.5) 6 L
12V/0.5A
BLACK(Φ0.5) 7 N EX-FAN
8
LAMP BLUE(Φ0.5) L
N TRANS-OUT SPEAK
BLUE(Φ0.5)
L
UV
N
SIXTEEN CORE CABLE
SOCKET L
LIGHT SOCKET L
N
RED(Φ1) FUSE3 RED(Φ1) SPEAKER
L
5A N
BLUE(Φ1)
YELLOW GREEN (Φ1.0) BROWN(Φ1.0)
RED(Φ1)
DOWN
DOOR MOTOR
BLUE(Φ1.0) DOWN-FAN N UP
N
L N
B SOCKET B
YELLOW GREEN(Φ1.0)
2.9 Wiring diagram
WAVE FILTER
10A DOWN FAN
POWER POWER LOCK
YELLOW GREEN (Φ1.0)
YELLOW GREEN(Φ1.0)
N BLUE(Φ1.0)
A A
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1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8
D T&H SENSOR D
FOOT SWITCH UP
WINDOW H
WINDOW L
BLACK(Φ1)
U V BALLAS T
BLACK(Φ0.5)
WHITE(Φ0.5)
UV LAMP WHITE(Φ0.5)
BLUE(Φ1)
RED(Φ0.5)
RED(Φ0.5)
WHITE(Φ0.5)
BLACK(Φ0.5)
BLUE(Φ0.5)
1 3
2
Window
H
L
GND
VCC
H T UV Ctrl
GND
BLUE(Φ0.5)
BSC-15IIA2-X Wiring diagram
up RED(Φ0.5)
Foot
YELLOW(Φ0.5) down
C BLACK(Φ0.5) C
LAMP YELLOW(Φ0.5)
220V/50Hz
ANJIAN
1 MCU EIGHT CORE CABLE
LCD
LIGHT BALLAST
2
RED(Φ0.5) GREEN(Φ1)
3
RED(Φ0.5) 4
BLUE(Φ1) TRANS-IN
5
BLACK(Φ0.5) 6 L
EX-FAN 12V/0.5A
BLACK(Φ0.5) 7 N
8
LAMP BLUE(Φ0.5) L
N TRANS-OUT SPEAK
BLUE(Φ0.5)
L
UV
N
SIXTEEN CORE CABLE
SOCKET L
LIGHT SOCKET L
N
RED(Φ1) FUSE3 RED(Φ1) SPEAKER
L
5A N
BLUE(Φ1)
YELLOW GREEN (Φ1.0) BROWN(Φ1.0)
RED(Φ1)
DOWN
DOOR MOTOR
BLUE(Φ1.0) DOWN-FAN N UP
N
L N
B SOCKET B
YELLOW GREEN(Φ1.0)
FUSE4
10A BLUE(Φ1) BLACK(Φ1) BROWN(Φ1)
YELLOW(Φ1) BLUE(Φ1)
BROWN(Φ1.0) FUSE2 DOOR MOTOR
L
WAVE FILTER
10A DOWN FAN
POWER POWER LOCK
YELLOW GREEN (Φ1.0)
YELLOW GREEN(Φ1.0)
N BLUE(Φ1.0)
A A
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1 2 3 4 5 6 7 8
3. Trouble shooting and Labels
3.1 Common faults & solution
3.1.1 Warning and reminder
Digital display of pressure difference, digital velocity display, audible and visual alarm system.
There will be audio and visual alarm when front window is lifting over safety height. Same time
LCD display will twinkle exclamation mark. Then just adjust the height of the front window.(Front
window height setting value is 200mm).
There will be audio and visual alarm if pressure of air supply filter or exhaust filter can’t meet
present value, at the same time LCD display will twinkle exclamation mark. Remind the operator to
replace the filter immediately to protect the operator’s safety.
There will be audio and visual alarm if the inflow velocity and down flow velocity below 20% of the
standard value, namely, inflow velocity below 0.42m/s, down flow velocity below 0.26m/s, at the
same time LCD display will twinkle exclamation mark to remind the operator pay attention.
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3.1.2 Trouble shooting
Please confirm whether the power is connected or not, whether the power cord is obvious damaged
or not, whether the fuse is good or not, and whether the power locks are in the open state or not
before the fault diagnosis.
Front window, fluorescent lamp and Check the front window, fluorescent lamp and the blower is
blower open or not.
Make sure the power connects well and the fuse is well
Front window Front window is open or not, blower works only when the
front window is open
Micro Switch Check if Micro Switch is broken or works fine
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Control panel Change it
Pressure or air speed Gas circuit Check whether gas circuit has dropped, is broken, or jammed
displayed incorrectly
Control panel Change it
Remote control Check if the Remote control is broken or not, and if there’s
Remote control doesn’t
work Connection cable Check whether main control panel and display board is
Micro switch Check whether the micro switch is good, and it works
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NOTES
(1) The above electrical parts must be operated by a qualified electrician in safety conditions
(cutting off power supply). The other parts are not allowed to remove; otherwise the user
should take responsibility by them;
(2) When failures are not occur, and the operator can’t solve, please notify our maintenance
department immediately. For your safety, please do not maintain equipment by yourself;
(3) The maintenance of this equipment is undertaken by trained and recognized technicians;
(4) If you need to order parts, contact the agent or our technical service department, and please
indicate the model and serial number of the cabinet purchased.
Socket, fan fuse (only BSC-15IIA2-X has fan fuse) are located in the top operation panel. When
replace them, turn off the power and disconnect plug, use a Phillips screwdriver counterclockwise
pressing screwed fuse holder, remove the fuse out and replace a new fuse, and then clockwise
pressing screwed fuse holder; FireWire fuse is located in the side of the cabinet operation panel, take
out of the fuse holder using a slotted screwdriver and replace with a new fuse, and then press it back
Picture 22
When replacing lights, make sure that the power is off, open the operation panel like shown in
Picture 23, use the control panel support frame (fixed in the inside position of the control panel as
shown), then like Picture 24 shown screwing the tube 90°, take the correspondence type of lamp, put
it to the lamp holder and screw 90 °to the right position.
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Picture 23
Picture 24
UV lamp should be replaced regularly according to the frequency of use, when using UV lamps
reach to the time of 600 hours, we recommend to replace the lamp. When replacing, first make sure
the power is off, and then screw the bulb 90 ° and take it off , then take the correspondence type of
lamp, and put it to the lamp holder and and screw 90 ° in reverse direction. After replacing the UV
lamp, it needs to keep pressing the button of UV for about five seconds when the machine stays
standby.
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Picture 25
Picture 27
Note:
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3)Ground label
41
8) Downflow filter upstream label
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4. Warranty
1) Warranty is 12 months from EX-factory date (excluding consumable accessories, UV and
Fluorescent lamp, fuse).
2) We will take no responsibility for risks caused by improper operation and man-made damages.
3) After the expiration of warranty, our company is also responsible for repairs, but the
corresponding maintenance cost should be charged.
4) Life time of biosafety cabinet is 8 years from production date on the label.
5) We can provide equipment drawings and necessary technical data for maintenance companies or
personnel trained by our company.
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