INSTRUCTIONS

CX33
Biological Microscope

This instruction manual is for the Olympus biological microscope.

To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating this
microscope, and always keep this manual at hand when operating this product. Optical Microscope and Accessory
Retain this instruction manual in an easily accessible place near the work desk for future
reference.
AX 8881
 In accordance with European Directive on Waste Electrical and Electronic Equipment, this
symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in
your country.

NOTE: This product has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the product is operated in a commercial environment. This product
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio communications.
Operation of this product in a residential area is likely to cause harmful interference in which case the
user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the product.

For Korea only

B급 기기 (가정용 방송통신기자재)

이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을

목적으로 하며, 모든 지역에서 사용할 수 있습니다.
Contents

Safety precautions ....................................................................................................................................... 1

1 Standard combination......................................................................................................................... 5

2 Nomenclature of operating portions ...................................................................................... 6

3 Outline of brightfield/darkfield observation methods ............................................. 7

4 Observation procedures.................................................................................................................... 9
1 Turning ON the LED illumination...................................................................................................................................... 9

2 Selection between the eyepiece light path and the camera light path ..................................... 9

3 Placing the specimen ............................................................................................................................................................ 10

4 Selecting the objective.......................................................................................................................................................... 14

5 Focusing on the specimen ................................................................................................................................................ 14

6 Adjusting the interpupillary distance ........................................................................................................................ 15

7 Adjusting the diopter. .............................................................................................................................................................. 16

8 Adjusting the aperture diaphragm (AS) ................................................................................................................ 17

9 Attaching the darkfield ring slit (for darkfield observation only) ....................................................... 17

10 Acquiring the image with the camera ..................................................................................................................... 18

11 Using the 100X oil immersion objective ................................................................................................................ 19

5 Glossary of optical performance terminology ............................................................. 21

6 Troubleshooting ...................................................................................................................................... 23

7 Specifications............................................................................................................................................ 25

8 List of optical performances ........................................................................................................ 26

9 Assembly....................................................................................................................................................... 27
9-1 Assembly diagram ......................................................................................................................................... 27

9-2 Assembly procedures ................................................................................................................................. 28

1 Removing the standard 10X eyepiece .................................................................................................................... 28

2 Attaching the eyepiece micrometer .......................................................................................................................... 28

3 Attaching the eyepieces (Standard 10X eyepieces or WHSZ15X-H) ........................................... 29

4 Attaching the objective CXPL20X or CXPL100XO ....................................................................................... 29

5 Attaching the specimen holder CX3-SHP or CX3-HLDT ......................................................................... 30

6 Attaching the camera adapter U-TV1XC and the camera ..................................................................... 30

7 Attaching the filter holder CH2-FH and darkfield ring slit CH2-DS .................................................. 31

8 Attaching the filter of 45mm in diameter .............................................................................................................. 31

9 Connecting the AC adapter and power cord ................................................................................................... 32

10 Attaching the allen wrench holder ............................................................................................................................. 33

 CX33
Safety precautions

If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
product may also be damaged. Always use the product according to this instruction manual.

The following symbols are used in this instruction manual.

CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury.
NOTE : Indicates a potentially hazardous situation which, if not avoided, may result in
damage to the product or other property.
TIP : Indicates the useful knowledge or information for use.

CAUTION - Prevention of infection -

Wear protective equipment such as gloves, etc.

When you observe the specimens which have potentiality of infection, wear protective equipment such as gloves, etc.
to prevent the specimens from touching your skin directly.
When you maintain the product which may have contacted the specimens which have potentiality of infection, wear
protective equipment such as gloves, etc., or clean the product before operation.
After observation, clean the portions where specimens contacted directly.
Remove the specimen when moving the product.
When moving this product, be sure to remove the specimen first, since it is in danger of dropping and spattering.

In case the specimen is damaged, promptly take the infection prevention measures.
When disposing of the product, follow the regulations and rules of your local government.
When disposing of the product which contacted the specimens which have potentiality of infection, follow the
regulations and rules of your local government.

CAUTION - Installation of the product -

Install the microscope on a sturdy, level table or bench.

For safety in particular, do not place a mat, etc. under the product.

CAUTION - Light from the objective -

Do not look directly at the light coming out from the objective or the light reflected from the specimen.
Be careful about the light coming out from the objective, since not only visible light but also light of invisible
wavelengths (such as ultraviolet and infrared) may be emitted depending on the illumination methods.

1
 CAUTION - LED (light emitting diode) -

Do not look directly at the light from LED for a long time.
If you feel that the light from LED is too bright during observation, adjust the light intensity using the brightness
adjustment knob before continuing the observation. The LED built in this product is basically eye-safe. However, do not
look directly at the light from LED for a long time while feeling too bright, since it may cause damage to your eyes.

CAUTION - Electric safety -

Always use the AC adapter and power cord provided by Olympus.

If the proper AC adapter and the power cord are not used, the electric safety and the EMC (Electro-Magnetic
Compatibility) performance of the product cannot be assured. If no power cord is provided, please select the proper
power cord by referring to the section “Proper selection of the power cord” at the end of this instruction manual.
Always connect the ground terminal.
Connect the ground terminal of the power cord and that of the power outlet. If the product is not grounded, our
intended electric safety and EMC performance of the product cannot be assured.
Do not use this product near the sources of strong electromagnetic radiation.
Proper operation may be interfered. Be sure to evaluate the electromagnetic environment before operating the product.
Disconnect the power cord in case of emergency.
In case of emergency, disconnect the power cord from the power cord connector of the product or from the power
outlet.
Install the product at the location where you can reach the power cord connector or the power outlet with your hand to
disconnect the power cord immediately.

This product complies with the emission and immunity requirements described in IEC61326 series.

CAUTION - Safety symbols -

The following symbols are attached to the product.

Study the meaning of the symbols and always use the product in the safest possible manner.

Symbol Meaning
Indicates a non-specific general hazard. Follow the description given after this symbol
or in the instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.

2
 CX33

Handling precautions

NOTE · This product is a precision instrument. Handle it with care

and avoid subjecting it to a sudden or severe impact.
· Never disassemble any part of the product. Otherwise,
failure could be caused.
· The objectives are screwed in tightly to prevent them from
being loosened during transportation. When removing the
objective, rotate it in the arrow direction as shown in the
picture on the left using the slip prevention rubber sheet,
etc.
· Be sure to use the specified objectives (objectives
provided with the microscope frame and CX-Plan series).
If incorrect objectives are combined, the performance
cannot be delivered.
· The tube of this microscope is removable, but do not
attach the tube of other microscopes.

1. Be careful not to apply the impact or let the dirt/dust attached to the microscope when carrying it.
2. Do not use the product in areas where it may be subjected to direct sunlight, high temperature and/or humidity, dust or
vibrations. (For conditions of operating environments, see “7 Specifications” on page 25.)
3. When installing the microscope, assure sufficient space around the microscope.
4. When carrying this microscope, disconnect the AC adapter from the microscope, and store the AC adapter and the power
cord in the microscope frame. Then, hold both sides of the arm as shown in the lower left picture, and carry it carefully. Then,
hold both sides of the arm a as shown in the picture on the left, and carry it carefully. (For procedures to store the AC
adapter and the power cord, see “Storing the AC adapter and power cord” on page 32.)

NOTE · Do not hold the stage b or the tube c , since they may
a be damaged.
· When carrying the microscope, be sure to remove the
specimen and filters in advance, since they are in a risk of
falling. Also, be careful not to touch the objective.
· Do not carry the microscope keeping the AC adapter
connected to the microscope. The AC adapter or the
microscope may be damaged by hitting the output
connector of the AC adapter.
· When carrying the microscope, be careful not to touch the
objective.

3
 Maintenance and storage

1. Do not leave stains or fingerprints on the lenses and filters. If they get dirty, blow away dust with a commercially
available blower and gently wipe the lens or filter with a piece of cleaning paper (or clean gauze). Only when cleaning
fingerprints and oil stains, slightly moisten a piece of cleaning paper with commercially available absolute alcohol
and wipe them off with it.

CAUTION Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away
from open flames or potential sources of electrical sparks. For example, the electrical equipment that
is switched ON and OFF may cause the ignition of a fire. Also, always use absolute alcohol only in a
well-ventilated room.
2. Wipe the portions other than the lens with a dry soft cloth. If the dirt cannot be removed by dry-wiping, moisten a soft
cloth with diluted neutral detergent and wipe the dirty surface with it.

NOTE Do not use organic solvents because they may deteriorate the coated surface or plastic parts.

3. After using this product, store it in a dry place or cover it with a dust cover. If you need the dedicated dust over (made
by Olympus), contact Olympus.

NOTE Do not use a highly sealable cover, such as a plastic bag, etc. as a dust cover. The humidity in the
microscope may increase to damage the product.

4. When disposing of this product, be sure to follow the regulations and rules of your local government. Contact Olympus
for any questions.

Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological
information at hospitals or laboratories. Typical field of applications are genetics, human blood and tissue examination,
neurology, pharmacology and cellular biology.
Do not use this instrument for any purpose other than its intended use.

This product complies with the requirements of directive 98/79/EC concerning in vitro
diagnostic medical devices. CE marking means the conformity to the directive.

This product is applied with the requirements of EMC standard IEC/EN61326-2-6 and
IEC/EN61326-1 concerning electromagnetic compatibility.

This product complies with the emission and immunity requirements described in IEC61326
series. The electromagnetic environment should be evaluated prior to operation of this product.

4
 CX33
1 Standard combination
Refer to the drawing below and make sure that all necessary units are included in the product you purchased.

Eyepieces
Tube

Objective

Power cord
Revolving nosepiece

Stage

Condenser

Microscope AC adapter
frame

Option units

· Eyepiece (2 pieces) · Filter holder · Darkfield ring · 20X objective

WHSZ15X-H CH2-FH CH2-DS 100X objective
· Eyepiece micrometer
(Ø24mm, Thickness: 1.5mm)
· Ø45mm filter
· Camera adapter · Camera · Specimen hold plate · Specimen holder
U-TV1xC DP22 or DP27 CX3-SHP CX3-HLDT

5
 2 Nomenclature of operating portions
Pre-focusing lever

Coarse focusing knob

Fine focusing knob

Binocular portion

Main switch
: Power is ON.
: Power is OFF.

Light path selection knob

Diopter adjustment rings

Revolving nosepiece

Specimen holder
Specimen holder fixing knobs

Coarse focusing knob

Fine focusing knob

Aperture diaphragm lever

Filter holder
(For darkfield observation)

Filter Y-axis knob

(The filter of 45mm in

diameter can be inserted.)
X-axis knob

Brightness adjustment knob

Tension adjustment ring

6
 CX33
3 Outline of brightfield/darkfield observation methods
    (Operation portion)      (Page)

1 Main switch 9 
Set the main switch to (ON) and adjust the brightness.
2 Brightness adjustment knob 9 

Select the light path. (Trinocular tube) 3 Light path selection knob 9

4 Specimen holder 10
Place the specimen on the stage.
5 X-axis/Y-axis knobs 12

Engage the 10X objective in the light path. 6 Revolving nosepiece 14

7 Coarse/fine focusing knobs 14

Bring the specimen into focus and adjust the brightness.
2 Brightness adjustment knob 9 

Adjust the interpupillary

distance. 8 Binocular portion 15

Adjust the diopter.

9 Diopter adjustment ring 16

For brightfield observation, adjust the aperture diaphragm. a Aperture diaphragm lever 17

Only for darkfield observation

Place the darkfield

b Filter holder 17
ring slit.

6 Revolving nosepiece 14
Engage the objective you use in the light path and bring
the specimen into focus. 7 Coarse/fine focusing knobs 14

Place the necessary filter. c Filter (Diameter:45mm) 31

Adjust the brightness. 2 Brightness adjustment knob 9 

Observation

7
 8

1
3

b
5

TIP Make a copy of this observation procedure guide and put it near the microscope to use for observation.

8
 CX33
4 Observation procedures
1 Turning ON the LED illumination

1 Set the main switch a to (ON).

2 Rotating the brightness adjustment knob b in the arrow direction

increases the brightness and rotating it in the opposite direction
b
decreases the brightness.

2 Selection between the eyepiece light path and the camera light path

You can select the light path for observing the image with eyepieces or the
light path for observing the image on monitors, etc. via camera.

1 Rotate the light path selection knob a of the trinocular tube to

select the light path. (Light intensity ratio: 100:0)
a
: Eyepiece

: Camera

NOTE Be sure to rotate the light path selection knob completely

until it stops to acquire the proper observation image.

9
 3 Placing the specimen

Specimen holder for observing one specimen

1 Rotate the coarse focusing knob a in the arrow direction to fully

b
lower the stage.
c
2 Press the specimen holding lever knob b backward (arrow direction)
to open the lever c , and slide the specimen from front to back on
the stage to place it.

3 After placing the specimen, return the specimen holding lever c

gently.
a
NOTE Place the specimen carefully. If you return the specimen
holding lever c with excess force or release your hand
from the specimen holding lever knob b in the middle,
the lever may collide with the specimen to damage it.

Placing the specimen with one hand

1 Rotate the coarse focusing knob a in the arrow direction to fully

lower the stage.
c 2 Place the specimen on the front side of the stage.
3 Spread the specimen holding lever c at the edge of the specimen
as shown in the picture on the left, and slide the specimen from front
to back on the stage to place it.

TIP For procedures to attach the specimen holder (option), see

page 30.

When using the specimen hold plate CX3-SHP (Option)

1 Rotate the coarse focusing knob a in the arrow direction to fully

b lower the stage.

c 2 Place the specimen on the resin sheet b .

NOTE · The outside of the aperture c of the resin sheet is not

illuminated by the light from the condenser, the specimen
cannot be observed.
When operating the X-axis knob, be careful not to cover
the aperture of the stage with the resin sheet.
a
· The specimen hold plate cannot be used in combination
with the 100X oil immersion objective because the optical
performances cannot be delivered sufficiently during
observation using the 100X oil immersion objective.
· The specimen hold plate cannot be used for the darkfield
observation because the shadow appears in the center of
the field of view during darkfield observation.

10
TIP For fixing the stage, see page 13.
 CX33

When using the specimen holder CX3-HLDT (Option)

1 Rotate the coarse focusing knob a in the arrow direction to fully

b lower the stage.

c 2 Press the specimen holding lever knob b backward (arrow direction)

to open the lever c , and slide the specimen from front to back on
the stage to place it.

3 After placing the first specimen until it touches the end, place the
second specimen so that it touches the first specimen.

a
4 After placing the specimens, return the specimen holding lever c
gently.

NOTE Place the specimen carefully. If you return the specimen

holding lever c with excess force or release your hand
from the specimen holding lever knob b in the middle,
the lever may collide with the slide glass to damage it.

Slide glass
It is recommended to use the following slide glasses a .

Length: 76 mm, Width: 26 mm, Thickness: 0.9 to 1.4 mm

b
(Thickness: 0.9 to 1.2 mm for darkfield observation)

NOTE Use the cover glass b with the thickness of 0.17mm to

fully deliver the performance of objective.

11
 Moving the specimen

· Rotating the upper Y-axis knob a moves the specimen in the Y-axis
a
direction (front and back).

· Rotating the lower X-axis knob b moves the specimen in the X-axis
direction (right and left).
b
Stage movable range: Depth 52 mm x Width 76 mm
NOTE · Do not move the specimen by touching the specimen
holder directly by hand, since it will damage the rotary
c mechanism of the knobs.
· The rotation torque of X-axis and Y-axis knobs become
heavier near the end of movable range of X-axis and
Y-axis. In this case, stop rotating the knob.
TIP · If the stage knob rubber c is attached and it is difficult to
operate the stage, remove the stage knob rubber.
If you want to change the height of the stage knob rubber, move
the stage knob rubber up and down to adjust it.
· When attaching the stage knob rubber, insert the larger one to
the Y-axis knob (upper side) and the smaller one to the X-axis
knob (lower side) from below.

Scale of specimen position

By reading and taking a note of X-axis and Y-axis scales of the observation
position on the specimen, you can return to the original observation position
easily even if the specimen is moved.

< Reading the scale >

1 Read the "0" position of the outside scale (vernier scale) by the inside
a
b scale (main scale). [ a ]
The scale indicates the unit of 1 mm.

2 Read the position where the outside scale line coincides with the
inside scale line by the outside scale (vernier scale). [ b ]
The scale indicates the unit of 0.1 mm.

TIP The position shown in the left picture can be read as

137.8 mm.

12
 CX33

Fixing the stage

If you want to move the observation position by moving the specimen with
your finger without using the specimen holder, the stage can be fixed so
that it does not move unexpectedly.

1 Move the X-axis/Y-axis knobs to match the hole a at the back right
c of the stage with the screw hole b .

2 Put the stage fixing knob c through the hole a , and screw it in the
screw hole b to fix the stage.
a NOTE The stage fixing knob is provided with the microscope.
Be sure to store the stage fixing knob in the safe place.
b

How to track an observation image

NOTE The up-down and left-right movements of the image

observed through eyepieces are opposite to the
movements of the specimen.

How to track the

image that moves
to the left (how to
move the image to
the right)? Move the
specimen to the
left.

Direction of moving the image

How to move
the image in the
lower area to the
upper area? Move the
specimen to the
front.

13
 4 Selecting the objective

1 Hold the revolving nosepiece a and rotate it so that the intended

objective comes exactly above the specimen.
NOTE · Do not rotate the revolving nosepiece by holding the
a objective.
· Be careful if you rotate the revolving nosepiece while
observing the edge of the slide glass with the high
magnification objective (40X, etc.), the objective may
interfere with the specimen holder.

TIP For attaching and removing the objectives, see page 29.

5 Focusing on the specimen

1 Rotate the coarse focusing knob a in clockwise direction (arrow

c direction) facing the microscope from right side to move the objective
c to the specimen as close as possible.

2 While observing the specimen through the eyepieces, slowly rotate

the coarse focusing knob a in the direction opposite to the arrow
to lower the stage.

b a
3 When the specimen comes into view, rotate the fine focusing knob
b to bring the specimen into focus precisely.

Adjusting the tension of the coarse focusing knob

The tension of the coarse focusing knob can be adjusted.

Insert a commercially-available flathead screwdriver into the groove c of
the tension adjustment ring. Rotating it clockwise (arrow direction) increases
the tension and counterclockwise decreases the tension of the coarse
focusing knob a .

a TIP If the stage descends by its own weight or the focus obtained
with the fine focusing knob b is lost soon, the tension is set
c
too loose. In this case, rotate the tension adjustment ring in
b
the arrow direction to increase the tension.

14
 CX33

Using the pre-focusing lever

The pre-focusing lever prevents the specimen from being damaged by

collision between the specimen and objective.

1 After bringing the specimen into focus with the high magnification
a objective, rotate the pre-focusing lever a in the arrow direction until
it stops.
2 To provide a certain margin for focusing range, rotate the pre-focusing
lever a by about half turn in the direction opposite to the arrow
direction from the stopped position.
NOTE If the pre-focusing lever is not positioned appropriately,
the stage may not rise and the specimen may not be
focused. In this case, adjust the pre-focusing lever
position again.
TIP It is recommended to use the pre-focusing lever to prevent the
specimen from damage, but if you do not use the pre-focusing
lever, rotate the pre-focusing lever in the direction opposite to
the arrow direction until it stops.

6 Adjusting the interpupillary distance

The interpupillary distance adjustment is to adjust the distance between

two eyepieces according to the distance between your eyes. This makes
a it possible to observe a single image and to reduce eye strain during
observation.

1 Keep the right and left eyepieces horizontally. While looking through
the eyepieces, move the binocular portion either in a or b direction
b
until the right and left fields of view coincide completely. The value
shown by the indicator (protrusion) c on the left side eyepiece sleeve
represents your interpupillary distance.

TIP · Note your interpupillary distance so that it can be easily

adjusted next time.
· You can adjust your interpupillary distance by moving the
binocular portion either to upper or lower side. Select the upper
side or the lower side according to your observation posture.

15
 7 Adjusting the diopter.

The diopter adjustment is to compensate for the difference in eyesights

of left and right eyes of the observer.

1 Rotate the diopter adjustment rings a of the right and left eyepieces
and move each scale “0” to each index b .

2 Engage the 10X objective in the light path and rotate the coarse/fine
b focusing knobs to bring the specimen into focus.

3 Change to the 40X objective, and rotate the coarse/fine focusing knobs
to bring the specimen into focus.

4 Change to the 10X objective. While looking through the right eyepiece
with your right eye, rotate the diopter adjustment ring a to bring the
specimen into focus.
In the same manner, while looking through the left eyepiece with
your left eye, rotate the diopter adjustment ring to bring the specimen
into focus.

a
5 Change to the 40X objective again, and rotate the coarse/fine focusing
knobs to bring the specimen into focus.

6 Change to the 10X objective, and check that the specimen is in focus
by looking through the right and left eyepieces.
TIP If the specimen is not in focus, repeat from 4 to 6 .

When the eyepiece micrometer is attached to the eyepiece

0 10 20 30 40 50 60 70 80 90 100 TIP For attaching the eyepiece micrometer, see page 28.

Eyepiece micrometer 1 While looking through the eyepiece equipped with the eyepiece
micrometer, rotate the diopter adjustment ring a to adjust so that
the scales and lines of the eyepiece micrometer can be viewed clearly.

2 Engage the 10X objective in the light path. While looking through the
eyepiece equipped with the eyepiece micrometer, rotate the coarse/
fine focusing knobs to bring the specimen into focus.

3 While looking through the eyepiece which is not equipped with the
eyepiece micrometer, rotate the diopter adjustment ring a to bring
the specimen into focus.

16
 CX33

Using the eye shades

When wearing eyeglasses

Use the eye shades in the normal, folded-down position.

When not wearing eyeglasses

Extend the folded eye shades in the arrow direction. Since the eye
shades prevent the unnecessary light from entering between eyepieces
and eyes, you can observe the specimen comfortably.

8 Adjusting the aperture diaphragm (AS)

The aperture diaphragm is a diaphragm to adjust the numerical aperture

of the condenser.
Adjusting the numerical aperture of the condenser appropriately
with respect to the numerical aperture of each objective allows
you to observe the specimen with the best contrast and resolution.
(For the aperture diaphragm, see page 22.)

1 Move the aperture diaphragm lever a to right and left direction to

match the index with the magnification of the objective in use.
a
NOTE For darkfield observation, open the aperture diaphragm.

9 Attaching the darkfield ring slit (for darkfield observation only)

For darkfield observation, the darkfield ring slit CH2-DS (option) and the filter
holder CH2-FH (option) must be attached to the condenser.

1 Insert the darkfield ring CH2-DS a into the filter holder CH2-FH b .
c 2 a 2 Push the filter holder b which includes the darkfield ring into the bottom
of the condenser c until the click sound is heard.
b 1 NOTE The darkfield observation is not available with the 100X oil
immersion objective.

17
 10 Acquiring the image with the camera

The observed image can be acquired by attaching the camera adapter and the digital camera for microscope to the
trinocular tube. (For attaching the camera adapter and the camera, see page 30.)

NOTE When using the camera adapter, be sure to adjust the parfocality. Otherwise, the image through
eyepieces and the image acquired by the camera are not focused at the same position.
For procedures to adjust the parfocality, refer to the instruction manual of the respective camera
adapter.

1 Rotate the light path selection knob a of the trinocular tube to move
it to position. (See page 9.)
a

TIP The image acquisition range is determined by the size of the

image sensor used in the camera and the magnification of
the camera adapter.
b The comparison between the observation range by standard
10X eyepieces and the image acquisition range by the
a
camera is shown in the picture on the left.

Observation a Digital camera for microscope (DP22)

range by Image sensor size (diagonal): 1/1.8-inch (9mm)
standard 10X
eyepieces
b Digital camera for microscope (DP27)
Image sensor size (diagonal): 2/3-inch (11mm)
When using the camera adapter 1 x (U-TV1XC)

18
 CX33

11 Using the 100X oil immersion objective

NOTE · Apply the immersion oil specified by Olympus to the tip of the 100X oil immersion objective.
Otherwise, the observed image is not in focus.
· Always use the immersion oil provided by Olympus.
Using the immersion oil which is not provided by Olympus cannot deliver the intended optical performance.
TIP For observation with the 100X oil immersion objective, it is recommended to apply the immersion oil between
the tip of condenser lens and the specimen as well prior to observation.
· If you apply the immersion oil between the tip of condenser lens and the specimen, the condenser of this
microscope delivers the best optical performance in the observation with the 100X oil immersion objective.
If you do not apply the immersion oil, the image observed with the 100X oil immersion objective becomes
slightly dark.
· For observation with 4X to 40X objectives, it is not necessary to apply the immersion oil between the tip of
condenser lens and the specimen. (The brightness of the observed image is same regardless of whether
the immersion oil is applied or not.) However, for observation by changing from 4X to 40X objectives to the
100X oil immersion objective, it is recommended to apply the immersion oil between the tip of condenser
lens and the specimen in advance.

1 (When applying the immersion oil between the tip of condenser lens
and the specimen)
Apply the immersion oil at the tip of condenser lens a first, and
place the specimen.

b a
2 Use the low magnification objective to bring the specimen into focus.

3 Apply the immersion oil on the observation position of the specimen

before engaging the 100X oil immersion objective into the light path.

4 Rotate the revolving nosepiece to engage the oil immersion objective

in the light path, and rotate the fine focusing knob to bring the
specimen into focus.
NOTE If the immersion oil contains air bubbles, the image will
be degraded. Make sure that the oil is free of air bubbles.
To remove bubbles, rotate the revolving nosepiece slightly
to move the oil immersion objective back and forth for
once or twice.

19
 5 After use, lower the stage and rotate the revolving nosepiece, and
remove the objective attached with the immersion oil from the
specimen.

6 Wipe off the immersion oil thoroughly from the tip of the objective
and the tip of the condenser lens with the cleaning paper or
the gauze slightly moistened with absolute alcohol. Wipe off the
immersion oil from the specimen in the same procedures.

NOTE · If you leave the immersion oil without wiping it off, the oil
may be adhered to cause the incorrect observation.
· The oil pan b can temporarily store the immersion oil
flowed down from the condenser lens. Clean the oil pan
regularly to prevent the immersion oil from overflow.
· If the immersion oil is overflowed from the oil pan b ,
b wipe off the immersion oil with the cleaning paper or
the gauze slightly moistened with absolute alcohol. If
the immersion oil is in the unreachable area, use the
tweezers to pick up the cleaning paper or the gauze and
wipe off the immersion oil.

CAUTION Follow the cautions indicated on the label of the

immersion oil.

20
 CX33
5 Glossary of optical performance terminology
Total magnification
The size of the specimen image to be observed is obtained by multiplying the eyepiece magnification by the objective
magnification. This value is referred to as the total magnification.

Example: Eyepiece (10X) x Objective (40X) = 400X

Resolution
The resolution is the ability of the lens to separate the image created by multiple proximal points.

The resolution is mainly determined by the ability of the objective and scarcely related to that of the eyepieces.

The function of the eyepieces is to just magnify an image already resolved by the objective.

Field number

The field number is the diameter of the image viewed through eyepieces indicated in millimeters.

Even with the same magnification, the larger the field number is, the larger field of view can be observed at a time.

Standard 10X eyepiece: 20mm

WHSZ15 x -H: 16mm

Actual field of view

The actual field of view is the size of the specimen actually viewed through eyepieces. This makes it possible to find out
the approximate size of the specimen.

Field number
Actual field of view =
Magnification of objective

Example : If the field number of the eyepiece is 20 and the magnification of the objective is 10X,

20
Actual field of view = = 2.0 mm
10

Approx. 1.0 mm, about half of actual field

of view.

Actual field of view: 2.0 mm

21
 Aperture diaphragm

The aperture diaphragm is a diaphragm to adjust the numerical aperture of the condenser.

Adjusting the numerical aperture of the condenser appropriately with respect to the numerical aperture of each objective
allows you to observe the specimen with the best contrast and resolution.

In general, since the contrast of the specimen to be observed 70-80%

with microscope is low, it is appropriate to adjust the 30-20%
numerical aperture of the condenser to approximately 70 to
80% of the numerical aperture of the objective.
The rough indication is that the aperture diaphragm image Aperture diaphragm image
viewed when looking into the tube without eyepieces looks as
the picture shown on the right. Pupil of objective
(bright circular ring)

Numerical aperture of objective and numerical aperture of condenser suitable for observation

Numerical aperture of Numerical aperture of condenser suitable for

Magnification objective observation of specimen for microscope
Objective
NA (when setting to 70% of objective)
Plan objective 4X 0.10 0.10 x 0.7 = 0.07
10X 0.25 0.25 x 0.7 = 0.175
20X 0.40 0.40 x 0.7 = 0.28
40X 0.65 0.65 x 0.7 = 0.455
100XO 1.25 1.25 x 0.7 = 0.875

22
 CX33
6 Troubleshooting
If problems occur, please review the following list and take remedial action as needed.
If you cannot solve the problem after checking the entire list, please contact Olympus for assistance.

Problem Cause Remedy Page

1. The brightness of observed field The light path selection knob of the Be sure to rotate the light path
of view is uneven. tube is not positioned correctly. selection knob completely until it 9
stops.

The objective is not engaged in the Rotate the revolving nosepiece until it
light path properly. clicks to engage the objective in the 14
light path.

The objective, eyepiece, condenser, Clean them thoroughly.

window lens or/and specimen are 4
dirty.

2. Dust or stains are visible in the The objective, eyepiece, condenser, Clean them thoroughly.
observed field of view. window lens or/and specimen are 4
dirty.

3. The observed image glares. The aperture diaphragm is narrowed Move the knob position of aperture
down too far. diaphragm lever to the position
17
of the same magnification as the
magnification of the objective in use.

4. The observed image is blurred The objective is not engaged in the Rotate the revolving nosepiece until it
in white or unclear. light path properly. clicks to engage the objective in the 14
light path.

The objective, eyepiece, condenser, Clean them thoroughly.

window lens or/and specimen are 4
dirty.

The immersion oil is not used with an Use the immersion oil.
19
oil immersion objective.

The immersion oil contains bubbles. Remove the air bubbles. 19

The specified immersion oil is not Use the immersion oil provided by
19
used. Olympus.

5. One-sided blur is found. The objective is not engaged in the Rotate the revolving nosepiece until it
The image looks as if flowing. light path properly. clicks to engage the objective in the 14
light path.

The specimen is not placed on the Place the specimen on the stage
stage properly. properly and secure it using the 10
specimen holder

6. The high magnification objective The specimen is placed upside Place the specimen with the cover
collides with the specimen down. glass side facing up.
10
before the specimen comes into
focus.

7. The tension of the coarse The tension adjustment ring is Loosen the tension adjustment ring to
14
focusing knob is too heavy. tightened too firmly. get proper tension.

8. The specimen is not in focus. The pre-focusing lever is positioned Raise its position.
15
(The stage cannot be raised.) too low.

23
 Problem Cause Remedy Page

9. The stage descends by its own The tension adjustment ring is Tighten the tension adjustment ring to
weight. loosened too much. get proper tension.
Or the focus is lost due to 14
slippage of the coarse focusing
knob.

10. The field of view is not matched The interpupillary distance is not Adjust it correctly.
15
between two eyes. adjusted correctly.

The difference in diopter of two eyes Correct it properly.

16
is not corrected properly.

Different eyepieces are used for right Use the same eyepiece for right side
29
side and left side. and left side.

11. When changing the objective The specimen is placed upside Place the specimen with the cover
10
from lower magnification to down. glass side facing up.
higher magnification, the
objective collides with the The cover glass is too thick. Use a cover glass with thickness of
0.17 mm. 10
specimen.

12. The LED illumination does not The AC adapter or power cord is Connect the power cord to a power
32
light. disconnected. outlet securely.

13. When changing the The diopter is incorrect. Adjust the diopter of eyepieces
magnification, the specimen is correctly. 16
defocused significantly.

Repair request
If you cannot solve the problems even though taking actions described in “Troubleshooting”, please contact Olympus
for assistance. Please provide us the following information at that time.
Product name and abbreviation (Ex.: Biological Microscope CX33RTFS2)
Product number
Phenomena

24
 CX33
7 Specifications
Item Specification
Optical system Infinity optical system
Microscope frame CX33RTFS2 / CX33LTFS2
Illumination system Built-in LED light source
Microscope frame (rated input power): 5 V 0.85 A
AC adapter (rated input power): 100-240 V 50-60 Hz 0.4 A
AC adapter (rated output power): 5 V 2.5 A
Focusing mechanism Stage height adjustment mechanism
Movement distance per scale of fine focusing knob: 2.5µm
Movement distance per rotation of fine focusing knob: 0.3mm
Movable range: 15mm (Upward by 1.5 mm and downward by 13.5mm from focal position)
Equipped with pre-focusing lever; Tension adjustable coarse focusing knob
Revolving nosepiece Fixed 4-hole revolving nosepiece
Tube Type Trinocular tube (fixed type)
Field number 20 (when using standard 10X eyepieces)
Tube tilting angle 30°
Interpupillary 48 to 75mm
distance
adjustable range
Light path 2 level selection: Eyepiece 100% or Camera100%
selection
Stage Movable range X direction: 76mm
Y direction: 52mm
Specimen holder CX33LTFS2 CX33RTFS2
Possible to hold 2 slide glasses Possible to hold 1 slide glass
Option CX3-SHP (Specimen hold plate) CX3-SHP (Specimen hold plate)
CX3-HLDT (Specimen holder)
Condenser Model type Abbe condenser
Numerical 1.25 (when immersed with oil)
aperture
Aperture Illumination aperture diaphragm blade, manual open/close type
diaphragm
Dimension / Weight 211 (W) x 390 (D) x 430 (H) mm / Approx. 7.0 kg (Not including AC adapter)
Operating environment · Indoor use
· Altitude: Max. 2000 meters
· Ambient temperature: 5 to 40 °C (41 to 104 °F)
· Humidity: Max. 80% (31 °C or less) (without condensation)
In case of over 31 °C (88 °F), the humidity in operating environment is decreased linearly
through 70% at 34 °C (93 °F), 60% at 37 °C (99 °F), and to 50% at 40 °C (104 °F).
· Supply voltage fluctuation: ±10 %
· Pollution degree: 2 (in accordance with IEC60664-1)
· Installation (overvoltage) category: II (in accordance with IEC60664-1)
Transportation / storage · Ambient temperature: -25 to 65°C (41 to 104 °F)
environments · Humidity: 0% to 90% (without condensation)

25
 8 List of optical performances
The following table shows the optical Magnification
performances when combining eyepieces and
Numerical aperture
objectives.
Mechanical tube
The picture on the right shows the various length
performances indicated on the objectives.

Field number
Color band
Cover glass thickness

Objective type (on the back side)

Plan objective

Optical Working Cover 10X Eyepieces (FN 20)

performance Numerical
distance glass Resolution Depth of Actual
Magnification aperture Total Remark
W.D. thickness (µm) focus field of
NA magnification
Objectives (mm) (mm) (µm) view
Plan objective 4X 0.10 27.8 - 3.36 40X 175.0 5.0
(FN 20) 10X 0.25 8.0 - 1.34 100X 28.0 2.0
20X 0.40 2.5 - 0.84 200X 9.27 1.1
40X 0.65 0.6 0.17 0.52 400X 3.04 0.5
100XO 1.25 0.13 - 0.27 1000X 0.69 0.2 Oil immersed

Glossary

Numerical aperture: The numerical aperture corresponds to F-number* of the camera and is related to the resolution. The
(NA) resolution becomes higher when the numerical aperture becomes larger.
(*: F-number is a value obtained by dividing the focal distance of lens by the valid aperture diameter.
This is used as an index to show the brightness of the lens.)

Working distance: Distance between the top surface of the cover glass and the tip of the objective.
(W.D.)

Resolution: The resolution is the ability of an objective to resolve adjacent two points in the image to the minimum
limit, which is expressed as the distance between two points on the specimen surface.

Field number: The field number is the diameter of the image viewed through eyepieces, indicated in millimeters.
(FN)

Total magnification: Magnification of objective x Magnification of eyepiece

Focal depth: The focal depth is the depth range of the specimen focused. The depth becomes deeper by
(Object side) narrowing down the aperture diaphragm and it becomes shallower by increasing the aperture
diaphragm of the objective.

Actual field of view: The actual field of view is a diameter of the field of view, expressed as the size (mm) on the specimen
surface.

26
 CX33
9 Assembly
9-1 Assembly diagram

The number in the following diagram indicates the order to attach each unit.
The detail assembly procedures are described on and after next page.

Camera
DP22 / DP27
(Option)

Standard 10X eyepiece Camera adapter

(detachable) U-TV1XC
(Option)
2

Eyepiece
6
1
WHSZ15X-H
(Option)

2
Eyepiece micrometer
(Option)

Specimen holder Objective

CX3-SHP/CX3-HLDT CXPL20X 10
(Option) CXPL100XO 4 Allen wrench
(Option)
holder

8 9
7
7
Microscope

Filter holder CH2-FH CX33RTFS2

(Option)

Darkfield ring slit

Filter
CH2-DS
Diameter: 45mm AC adapter 9 Power cord
(Option)
(Option)

For darkfield observation

Filter
Diameter: 32.5mm
(Option)

27
 9-2 Assembly procedures

1 Removing the standard 10X eyepiece

TIP The standard 10X eyepieces are clamped with screws.

1 Loosen the clamping screws a of the 10X eyepieces using the small
flathead screwdriver and remove the eyepieces.

2 Attaching the eyepiece micrometer

TIP The size of the eyepiece micrometer which can be attached to eyepieces of this product is 24 mm in diameter and
1.5 mm in thickness.
1 To attach the eyepiece micrometer, loosen the clamping screw of the
right side eyepiece using a small flathead screwdriver and remove
b the eyepiece.

2 Hold the eyepiece frame, loosen the reticle holder a and remove
3 the reticle holder a .

3 Insert the eyepiece micrometer b in the removed reticle holder a

with the display surface of the micrometer facing down.
4
a NOTE Be careful not to leave dirt on the eyepiece micrometer b ,
since it will be noticeable during observation.
4 Screw in the reticle holder a which includes the eyepiece micrometer
b to the bottom of the eyepiece gently.

NOTE Be sure to screw in the reticle holder a completely until

it stops.
5 Attach the eyepieces to the tube, and tighten the clamping screws.
4

28
 CX33

3 Attaching the eyepieces (Standard 10X eyepieces or WHSZ15X-H)

1 Insert WHC15X-H into the eyepiece sleeves and tighten the clamping
screws a using the flathead screwdriver.

4 Attaching the objective CXPL20X or CXPL100XO

1 Screw in the objective to the mount hole of the revolving nosepiece

completely by rotating it in the arrow direction paying attention not
to drop it.

Removing the objective

1 If the objective is screwed in too firmly, use the slip prevention rubber
sheet, etc. to rotate the objective in the direction opposite to the
attaching direction (arrow direction).

TIP When attaching the objective CXPL20X, remove the 40X objective
first, and then attach objectives in the order of 4X, 10X, 20X and
40X.

29
 5 Attaching the specimen holder CX3-SHP or CX3-HLDT

A 1 Rotate the coarse focusing knob to fully lower the stage.

b c a
2 Loosen the specimen holder fixing knob a (2 pcs.) of the stage
plate and remove the standard specimen holder by pulling it toward
front side.

TIP You can also loosen the specimen holder fixing knob a by
inserting the coin into the groove of the specimen holder fixing
knob a and rotating it in counterclockwise direction.

3 When using the specimen hold plate CX3-SHP

Put the metal plate c over the resin sheet (one sheet only) b by
B a matching respective U-shape grooves, and engage U-shape grooves
b
to the screws a . (Picture A shown on the left)

TIP Be sure to peel off the protection sticker attached to both

surfaces of the resin sheet b before use.

When using the specimen holder CX3-HLDT

Insert the U-shape groove of the specimen holder b in the specimen
holder fixing knob a . (Picture B shown on the left)

4 While pushing the specimen holder toward the back (arrow direction),
tighten the specimen holder fixing knob a (2 pcs.).

TIP You can also tighten the specimen holder fixing knob a by
inserting the coin into the groove of the specimen holder fixing
knob a and rotating it in clockwise direction.

6 Attaching the camera adapter U-TV1XC and the camera

TIP When attaching the camera adapter, also refer to the instruction manual of the camera adapter in use.

NOTE Be sure to use the specified camera adapter and the camera. Using those not specified cannot
ensure the stability of the microscope.

1 Attach the camera to the camera adapter.

1
2 Loosen the clamping screws a of the camera adapter of the trinocular
tube sufficiently using the provided allen wrench, and remove the
cap b .
b
3 3 Insert the camera adapter into the camera adapter attaching portion
a of the trinocular tube.

4 Tighten the camera adapter clamping screws a completely.

30
 CX33

7 Attaching the filter holder CH2-FH and darkfield ring slit CH2-DS

For details, see page 17.

8 Attaching the filter of 45mm in diameter

If necessary, insert the filter a in the filter holder (window lens) at the base
a
of the microscope frame.

TIP One to several filters of 45mm in diameter can be inserted in stack.

(Insertable thickness: up to 1.6mm)

NOTE If it is difficult to attach the filter due to the narrow space,

rotate the coarse focusing knob to raise the stage.

31
 9 Connecting the AC adapter and power cord

CAUTION · Always use the AC adapter and power cord provided by Olympus. If the proper AC adapter and the
power cord are not used, the electric safety and the EMC (Electro-Magnetic Compatibility) performance
of the product cannot be assured. If no power cord is provided, please select the proper power cord by
referring to the section “Proper selection of the power cord” at the end of this instruction manual.
NOTE · The power cord and AC adapter cord are vulnerable when bent or twisted. Never subject them to
excessive force.
· Be sure to set the main switch a to (OFF) before connecting the AC adapter and the power cord.

1 Connect the output connector b of the AC adapter to the input

connector c on the rear of the microscope.

c NOTE Do not tilt the microscope to backward. Otherwise, the

output connector b may be damaged.
a
1 2 Connect the connector portion d of the power cord to the connector
e completely.
b
2
3 Connect the power cord’s plug f to the power outlet g on the wall.

e
d

f
3

Storing the AC adapter and power cord

NOTE Be sure to set the main switch to (OFF) before storing
the AC adapter and the power cord.

1 Disconnect the power cord’s plug from the power outlet on the wall.
e
2 Disconnect the output connector a of the AC adapter from the input
connector b on the rear of the microscope.

3 Bundle the AC adapter cord c and store it at the back of the

microscope together with the AC adapter d and the power cord e .

a
d

b
c

32
 CX33

10 Attaching the allen wrench holder

TIP · It is recommended to attach the provided allen wrench holder

a a to the back of the microscope frame.

· The provided allen wrench b can be fixed to the provided

b
allen wrench holder a .

33
 Proper selection of the power supply cord
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to
“Specifications” and “Certified Cord” below:
Caution : In case you use a non-approved power supply cord for Olympus products, Olympus can no longer
warrant the electrical safety of the equipment.

Specifications

Voltage rating 125 V AC (for 100-120 V AC area) or, 250 V AC (for 220-240 V AC area)
Current rating 6 A minimum
Temperature rating 60 ˚C minimum
Length 3.05 m maximum
Fittings configuration Grounding type attachment plug cap. Opposite terminates in molded-on IEC
configuration appliance coupling.

Table 1 Certified cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with
an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of the agencies
listed in Table 1. In case you are unable to buy locally the power supply cord which is approved by one of the agencies
mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in your
country.

Country Agency Certification mark Country Agency Certification mark

Argentina IRAM Italy IMQ

Australia SAA Japan JET, JQA,

Austria ÖVE Netherlands KEMA

Belgium CEBEC Norway NEMKO

Canada CSA Spain AEE

Denmark DEMKO Sweden SEMKO

Finland FEI Switzerland SEV

United ASTA
France UTE
Kingdom BSI

Germany VDE U.S.A. UL

Ireland NSAI

34
 CX33

Table 2 HAR flexible cord

Approval organizations and cordage harmonization marking methods

Alternative marking utilizing

Printed or embossed black-red-yellow thread (Length
harmonization marking (May be of color section in mm)
Approval organization
located on jacket or insulation of
internal wiring) Black Red Yellow

Comite Electrotechnique Belge

CEBEC <HAR> 10 30 10
(CEBEC)

Verband Deutscher Elektrotechniker

<VDE> <HAR> 30 10 10
(VDE) e.V. Prüfstelle

Union Technique de I´Electricite´

USE <HAR> 30 10 30
(UTE)

Instituto Italiano del Marchio di

IEMMEQU <HAR> 10 30 50
Qualita´ (IMQ)

British Approvals Service for Electric

BASEC <HAR> 10 10 30
Cables (BASEC)

N.V. KEMA KEMA-KEUR <HAR> 10 30 30

SEMKO AB Svenska Elektriska

SEMKO <HAR> 10 10 50
Materielkontrollanstalter

Österreichischer Verband für

<ÖVE> <HAR> 30 10 50
Elektrotechnik (ÖVE)

Danmarks Elektriske Materialkontroll

<DEMKO> <HAR> 30 10 30
(DEMKO)

National Standards Authority of Ireland

<NSAI> <HAR> 30 30 50
(NSAI)

Norges Elektriske Materiellkontroll

NEMKO <HAR> 10 10 70
(NEMKO)

Asociacion Electrotecnica Y
<UNED> <HAR> 30 10 70
Electronica Espanola (AEE)

Hellenic Organization for

ELOT <HAR> 30 30 70
Standardization (ELOT)

Instituto Portages da Qualidade

np <HAR> 10 10 90
(IPQ)

Schweizerischer Elektro
SEV <HAR> 10 30 90
Technischer Verein (SEV)

Elektriska Inspektoratet SETI <HAR> 10 30 90

Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG

Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

35
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