Physico Chemical Properties of Flour and Starch From Jackfruit Seeds (Artocarpus Modified Starches
Physico Chemical Properties of Flour and Starch From Jackfruit Seeds (Artocarpus Modified Starches
Physico Chemical Properties of Flour and Starch From Jackfruit Seeds (Artocarpus Modified Starches
Kamontip Sajjaanantakul
Jackfruit (Artocarpus heterophyllus Lam.) is one of the most popular tropical fruits grown in Asia.
The objective of this study was to compare physico‐chemical properties of native flour and
starch from jackfruit seeds (A. heterophyllus) to commercially modified starches (Novation 2300
and Purity 4). The colour of jackfruit seed starch was lighter than the Novation 2300 starch but
darker than the Purity 4 starch. The jackfruit seed starch had a narrower gelatinization
temperature range than Purity 4 and required less gelatinization energy compared with modified
starches. The peak viscosity of jackfruit seed starch was lower than commercially modified
starches. Likewise, setback viscosity, swelling power and solubility of jackfruit seed starch
showed similar trends. Results from this study suggest that native starch from jackfruit seed
could be used as an alternative for modified starches in a system needing starch with a high
thermal and/or mechanical shear stability.
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2621.2004.00781.x
Some physicochemical and rheological properties of jackfruit seed flour and starch, isolated from the
flour were investigated. The flour had good capacities for water absorption (205%) and oil absorption
(93%). Substitution of wheat flour with the seed flour, at the level of 5, 10 and 20% markedly reduced
the gluten strength of the mixed dough. The Brabender amylogram (6% concentration, db) of seed
starch showed that its pasting temperature was 81 °C and its viscosity was moderate, remained constant
during a heating cycle and retrograded slightly on cooling. The starch showed an A-typed X-ray powder
diffraction pattern. KEYWORDS: Jackfruit Flour, Jackfruit Starch, physicochemical and rheological
properties. ScienceAsia 28 (2002) : 37-41 INTRODUCTION Jackfruit (Artocarpus heterophyllus Lam) is
popular fruit crop that is widely grown in Thailand and other tropical areas. The ripe fruit contains well
flavored yellow sweet bulbs and seeds (embedded in the bulb). The edible bulbs of ripe jackfruit are
consumed fresh or processed into canned products. Seeds make-up around 10 to 15% of the total fruit
weight and have high carbohydrate and protein contents (Bobbio et al 1978 and Kumar et al 1988).
Seeds are normally discarded or steamed and eaten as a snack or used in some local dishes. As fresh
seeds cannot kept for a long time, seed flour can be an alternative product, which be used in some food
products. There have been few studies on jackfruit seeds. Bobbio et al (1978) reported some
physicochemical properties, such as pasting characteristics of jackfruit seed starch. Kumar et al (1988)
studied the proximate compositions of two varieties of jackfruit seeds and reported considerable
biochemical difference between the two varieties. The starch content of the seed increases with
maturity (Rahman et al 1999). Different locations give different seed contents. Some functional
properties of jackfruit seed flour and its protein digestibility was reported by Singh et al (1991). This
work investigated some physicochemical and rheological properties of the flour and the isolated starch
from a local jackfruit variety called Thong – Sud – Jai. This variety is of local origin in Prachinburi
Province. It is widely grown in Thailand and gives a high fruit yield (Yospunya, 1999). The type of starch
analyzed by the X-ray powder diffraction technique, the first reported use of this technique to study
jackfruit starch. Additionally, bread dough substitution with jackfruit seed flour was also investigated.
MATERIAL AND METHODS Sample preparation Jackfruit (Artocarpus heterophyllus Lam) variety Thong
Sud Jai seeds from a local market in Bangkok, Thailand was used for this study. The seeds (3 kgs) were
cleaned and the white aril (seed coats) were peeled off. Seeds were then devided into two parts. One
part was lye-peeled with 5% NaOH for 2 min to remove the thin brown spermoderm covers the fleshy
white cotyledons. The seeds were sliced into thin chips and tray dried at 50° - 60 °C until their moisture
content was less than 13%. The chips were ground in a Pin mill FFC-23 to a 70 - mesh flour, packed in
plastic pouches and stored in a refrigerator (< 10 °C. This 38 ScienceAsia 28 (2002) step was repeated
several times until the supernatant was clean and clear. The starch was dried in a convection oven at 40
° to 60 °C until the moisture content was less than 13%, then ground with a mortar and pestle and
passed through a 70 µm sieve. Sample was stored in air tight containers at room temperature until use.
Chemical analyses. Representative samples of flour were analyzed in triplicate for moisture, crude
protein (conversion factor N x 5.7), crude lipid, and crude fiber ash contents using standard methods of
the Association of Official Analytical Chemists (AOAC) (1990). Total starch was measured following the
polarimetric procedure of Meloan and Pomeranz (1973). The Specific rotation, [α] = 203, which is
arbitrarily taken as value for all starch was used for calculation (AOAC 1990). The pH of the flour was
measured by the potentiometric method following the standard procedure in AOAC 1990. Nitrogen
content of jackfruit seed starch was analyzed by a macro-Kjeldahl method (AOAC, 1990). Four grams of
the starch was used for the analysis. Protein content was calculated by multiplying the nitrogen content
by 5.7. Amylose contents of jackfruit seed starch was determined in triplicate using the
spectrophotometric method described by Javis and Walker (1993). Standard potato amylose and
amylopectin (Sigma Chemical) were used as references. Physical and functional properties Granular
morphology of the isolated starch granules was identified in a light microscope. Scanning Electron
Microscopy analysis of the isolated starch was performed in an SEM (JOEL Tokyo, Japan) with
magnification of 1100X. X-ray diffraction: step-scanned X-ray powder diffraction patterns for jackfruit
starch were collected on the finely ground samples on a Shimadzu 610 diffractometer (Shimadzu, Tokyo,
Japan). The X-ray source operated at 30 kV and 40 mA with a Cu target and graphite – monochromator
radiation Kα radiation (λ = 1.5406). Data were collected by stepscanned method between 2 ° to 60 ° in
2θ with a step size of 0.02° 2θ and a counting time of 2 sec/step. Water and oil absorption capacities of
the flour were measured in triplicates by the method of Sosulski et al (1987), using 0.5 g flour (dry
weight basis, dwb) in 15 ml centrifuge tubes. Brabender viscosity curves of flour and starch at 8%
concentration (dwb) were determined according to the procedure described by Mazur et al (1957).
Standard procedures using the Brabender farinograph (Brabender OHG Duisburg, model 82575000) and
extensograph (Brabender OHG Duisburg, model 850000) were employed to determine the rheological
properties of the doughs. White breads were prepared by a straight dough method using the standard
procedure in AACC (1983). All purpose wheat flour (protein 10-11%) were supplemented with three
different levels (5,10, 20%) of jackfruit seed flour. Specific volume (cm3 / 100gm) of the breads was
determined by the seed dis-placement method. RESULT AND DISCUSSION Flour The flour composition of
this study, compared with Singh et al (1991) were shown in Table l. The major components of the flours
were carbohydrates (78.0%). The proximate analysis showed that protein (11.2%, dwb) and lipid (0.99%,
dwb) contents of the flour were lower than those reported by Singh et al (1991). The difference can be
contributed by variety difference, maturation of the seeds and environmental conditions. These effects
were already reported by Rahman et al (1999). Composition of flour depends on nature of the seeds.
Bobbio et al (1978) reported protein, crude lipids and carbohydrates contents of jackfruit seeds as
31.9%, 1.3% and 66.2% (dwb), respectively. The protein content reported by Bobbio et al was very high;
however, the seeds were reported to have been collected from fruits of various trees and no variety was
reported. Kumar et al (1988) also reported composition of seeds from two varieties of jackfruit. Protein,
crude lipids and carbohydrates content were 17.8-18.3%, 2.1-2.5% and 76.1% (dwb), respectively. If the
flour was prepared from seeds without removing the thin brown spermodesm, the crude fiber content
was 2.36% (Table 1), close to that reported by Singh et al (1991). However, the flour used in this study
was prepared only from lye-peeled seeds to remove the brown coating. Water absorption capacity and
oil absorption capacity of the flour were reported in Table l. These indicated good ability of the flour to
bind water and lipid. The water absorption in this study was higher than that reported by Singh et al
(1991). Milling equipment and flour preparation methods, such as a milling time can effect some
properties of the flour; for example, starch damage, which will result in high water absorption because
water can penetrate into granules more easily than intact granules. ScienceAsia 28 (2002) 39 Since the
seed flour had high protein content (11%), the possibility of substitution of wheat flour with the seed
flour for bread making was investigated. Table 2 showed the rheological tests of jackfruit seed flour
mixed with wheat flour. With increasing level of supplementation, the water absorption capacity
increased. High water absorption capacity is a characteristic of the wheat flour which is employed for
bread making (Kent, 1975). The bread dough peak time and dough stability time were reduced as
supplementation increased. This confirmed the reduction of protein gluten strength in the wheat/ seed
flour. The mixed dough also showed a poor mixing tolerance index (viscosity difference after 5 min of
peak viscosity). With more supplementation, the mixing tolerance index increased. Extensograph also
indicated that the dough had poor extensibility and resistance to extensibility as compared to normal
wheat dough. As the results, the specific baking volume of the bread was reduced by 51% at 5%
supplementation level of wheat flour with jackfruit seed flour (Table 2). Starch properties Scanning
electron microscopy (SEM) and optical microscopy under normal light showed round and bell-shaped
granules predominate (Figure 1). Optical microscopy with polarized light showed sharp dark maltese
cross in all starch granules. The hilum is central (Figure 2). The birefringence is different from that of the
faint polarization cross reported by Bobbio et al. Pasting properties of 8% jack-fruit seed starch was
studied by means of the Brabender viscoamylograph (Table 3). Tapioca and corn starches, which are
commonly used in food industry, are also shown Table 1. Composition and some physicochemical
characteristics of jackfruit flour (% dry weight basis, except moisture). Determination % (dwb) With
brown spermoderm Without brown spermoderm Singh et al (1991) Moisture 7.70 ±0.20 8.57±0.25 5.1
Crude protein (%Nx5.7) 11.02±0.46 11.17±0.21 17.2 Crude lipid 1.01±0.12 0.99±0.08 2.2 Crude fiber
2.36±0.04 1.67±0.11 3.06 Ash 3.97±0.04 3.92±0.03 3.6 Total Carbohydrate (a) 81.64 82.25 74 Total
starch 77.76±0.96 - Amylose content of starch 32.05±1.20 - Protein content of starch 1.84 - pH 5.68 -
Water absorption capacity(%) 205 141 Oil absorption capacity (%) 92.6 90.2 (a) determined by
difference Table 2. Rheological tests of flours and specific volumes of white bread. Parameter WF JF 5%
JF 10% JF 20% WF 95% WF 90% WF 80% % Water absorption 66.6 66.0 66.3 66.9 Arrival time (min) 1.7
1.7 2.0 1.7 Peak time (min) 8.6 4.9 4.7 3.4 Stability time (min) 11.4 6.8 5.3 4.1 Mixing tolerance index
(BU) 51 100 118 178 Extensibility (cm) 9 11.1 9.9 13.3 Resistance extensibility (BU) 1000 740 523 185
Specific volume of bread cm3/100 gm 8.64 4.21 3.51 3.21 WF, wheat flour, JF, jackfruit seed flour ; BU,
Brabender unit 40 ScienceAsia 28 (2002) for comparison. The lower breakdown of viscosity (P/F value)
during heating cycle for jackfruit seed starch as compared to that of tapioca starch indicated good
stability of the starch paste and good bonding forces within the starch granules (Zobel et al, 1984). The
starch paste also showed lower retrogradation as compared with tapioca and corn starch pastes.
Amylose content of jackfruit seed starch was 32%, (Table 1), higher than the mean value found in
tapioca starch (17%) and corn starch (26%). However, the starch paste showed relatively low
retrogradation. This is probably due to differences in amylose molecular weight and its ability to leach
out of the starch granules (Zobel, 1984). The jackfruit starch is in an amorphous state as seen from the
broad XRD peak pattern presented in Figure 3. The XRD pattern of jackfruit is different to the patterns of
potato and water chestnut starch, especially in position and relative peak intensity in the range of 2θ = 5
°-6 °, but it is similar to the pattern of waxy rice (Hizukuri, 1988). The absence of the peak of 2θ = 5 °-6 °
is characteristic of type-A starch, Therefore, the jackfruit seed starch belongs to A-type starch as do
cereal starches in general belong to the A-type pattern (Zobel, 1964). Cheetham and Tao (1998) also
reported waxy maize starch (amylose 0%) and maize starch (amylose 28%) showed a typical A-type
pattern. Transition from A-type to Btype pattern occurrs at amylose about 40%. CONCLUSION Flour
from jackfruit seed was prepared by dry milling. It is high in protein and carbohydrate contents. The
flour has good water and oil absorption abilities. However, less than 5% of wheat-flour can be replaced
with jackfruit seed flour for making white bread. Further studies are required to improve the seed flour
quality for usage. Starch was also isolated from the flour. Its amylose content was high. The starch
showed good paste stability during heating and gave the A-type X-ray diffraction pattern. REFERENCES 1.
AACC (1983) Approved Methods of the American Association of Cereal Chemists. AACC. St Paul, Minn. 2.
AOAC (1990) Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists.
Washington, DC. Table 3. Pasting properties of jackfruit – seed tapioca and corn starches. Pasting
properties % (db) Jackfruit Starch 8% Tapioca 8% Corn 8% Pasting temperature (°C) 81 61 73 Peak
viscosity (BU)(a) (P) 798 1590 750 Final viscosity @ 95 °C 20 min (BU) (F) 680 400 700 Cooled to 50 °C
(BU) ( C ) 820 690 1350 Breakdown (P / F) 1.17 3.98 1.07 Retrogradation (C / F) 1.21 1.73 1.93 (a) (BU) =
Brabender Unit Fig 1. Scanning electron micrograph (1100x) of jackfruit seed (Artocarpus heterophyluss
L) starch. Fig 2. Photomicrograph of jackfruit seed starch under polarized light. ScienceAsia 28 (2002) 41
3. Bobbio FO, El-Dash AA, Bobbio PA and Rodrigues L R (1978) Isolation and characterization of the
physicochemical properties of the starch of jackfruit seeds (Artocarpus heterorphyllus). Cereal Chem 55,
505-11. 4. Cheetham NWH and Tao L (1998) Variation in crystalline type with amylose content in maize
starch granules: an X-ray powder diffraction study. Carbohydrate Polymers 36, 277-84. 5. Hizukuri S,
Takeda Y, Shitaozono T, Abe J, Ohtakara A, Takeda C, Saga C and Suzuki A (1988) Structure and
properties of water chestnut (Trapa natans L var bispinosa Makino) starch. Starch/Starke, 40, 165-71. 6.
Javis CE and Walker JRL (1993) Simultaneous, rapid, spectrophotometric determination of total starch,
amylose and amylopectin. J Sci Food Agric 63, 53-7. 7. Kent HL (1975) Technology of Cereals 2nd ed
Flour Milling and Baking Association. 8. Kumar S, Singh AB, Abidi AB, Upadhyay RG and Singh A (1988)
Proximate composition of jack fruit seeds. J Food Sci Techno 25, 308-9. 9. Mazurs E, Schoch TJ and Kite
FE (1957) Graphical analysis of the Brabender viscosity curves of various starches. Cereal Chem 34, 141-
52. 10.Meloan CF and Pomeranz Y (1973) Food Analysis Laboratory Experiments. AVI Publishing
Company, Inc, Westport, Connecticut. 11.Rahman MA, Nahar N, Mian AJ and Mosihuzzaman M (1999)
Variation of carbohydrate composition of two forms of fruit from jack tree (Artocarpus heterophyllus L)
with maturity and climatic conditions. Food Chem 65, 91-7. 12.Singh A, Kumar S and Singh IS (1991)
Functional properties of jack fruit seed flour. Lebensm – Will u Technol 24, 373-4. 13.Sosulski FW and
McCurdy AR (1987) Functionality of flours, protein fractions and isolates from peas and faba bean. J
Food Sci 52, 1010-4. 14.Pranichaya Y (1999) Kanoon 3rd ed, Matichon Pub Comp, Bangkok, Thailand.
15.Zobel H F (1984) Gelatinization of Starch and Mechanical Properties of Starch Pastes. In Starch:
Chemistry and Technology. (Edited by Whistler, R L, BeMiller J N and Paschall E F ), pp 300-302.
Academic Press, New York. 16.Zobel H F (1964) X-ray Analysis of Granular Starches. In Methods in
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https://www.redalyc.org/pdf/3032/303226530005.pdf
Cheri Bantilan
Nutrition
Benefits
Downsides
Uses
Bottom line
Jackfruit is a fruit found in many parts of Asia.
It has been gaining popularity due to its delicious, sweet taste and various
health benefits.
However, the flesh isn’t the only part of the fruit you can eat — a single
jackfruit may contain 100–500 edible and nutritious seeds (1).
Calories: 53
Carbs: 11 grams
Protein: 2 grams
Fat: 0 grams
Fiber: 0.5 grams
Riboflavin: 8% of the Reference Daily Intake (RDI)
Thiamine: 7% of RDI
Magnesium: 5% of RDI
Phosphorus: 4% of RDI
Fiber and resistant starch have been linked to many powerful health benefits,
including hunger control, reduced blood sugar levels, and improved digestion
and insulin sensitivity
Modern research has found that they may have other helpful properties.
In fact, studies have shown that jackfruit seeds may have antibacterial effects
(2Trusted Source).
One study found that the surface of jackfruit seeds is covered with small
particles that act as antibacterial agents.
Some studies suggest that jackfruit seeds may have several anticancer
properties. This is believed to be due to their impressive levels of plant
compounds and antioxidants.
Studies have shown that these plant compounds may help fight inflammation,
enhance your immune system, and even repair DNA damage (12Trusted
Source, 13Trusted Source).
A recent test-tube study found that jackfruit seed extract reduced the
formation of cancerous blood vessels by 61% (14).
Similarly to other seeds, jackfruit seeds contain both soluble and insoluble
fiber.
Fiber passes through your digestive tract undigested and helps normalize
bowel movements by adding bulk to your stools, making them softer and
increasing frequency.
What’s more, fiber is considered a prebiotic, which means it helps feed the
beneficial bacteria in your gut (2Trusted Source, 3, 15).
These beneficial gut bacteria help support healthy digestion and immune
function (16Trusted Source, 17Trusted Source).
Numerous studies have found that increasing fiber intake can help relieve
constipation (18Trusted Source).
Furthermore, fiber may help protect against inflammatory bowel disease and
relieve symptoms of hemorrhoids (19Trusted Source, 20Trusted
Source, 21Trusted Source).
Elevated levels of LDL (bad) cholesterol are associated with high blood
pressure, diabetes, and an increased risk of heart disease (23Trusted
Source, 24Trusted Source).
Contrarily, higher levels of HDL (good) cholesterol have been found to have
heart-protective effects (25Trusted Source).
A study in rats found those that ate more jackfruit seeds had reduced levels of
LDL (bad) cholesterol and increased HDL (good) cholesterol, compared with
rats that ate fewer jackfruit seeds (26).
Individuals who are taking medications that increase the risk of bleeding
should be cautious of consuming jackfruit seeds.
In one study, jackfruit seed extract showed the ability to slow blood clotting
and even prevent clots from forming in humans (27).
Therefore, jackfruit seeds may further increase the risk of bleeding when
taken with these drugs. If you’re taking medications that may increase the risk
of bleeding, you should avoid consuming jackfruit seeds (3).
aspirin
blood thinners (anticoagulants)
antiplatelet drugs
nonsteroidal anti-inflammatory drugs (ibuprofen or naproxen)
Contain antinutrients
Raw jackfruit seeds contain powerful antinutrients called tannins and trypsin
inhibitors. These can interfere with nutrient absorption and digestion (1, 3).
Tannins are a type of polyphenol that’s commonly found in many plant foods.
They can bind to minerals, such as zinc and iron, and form an insoluble mass,
reducing your ability to absorb these minerals (28Trusted Source, 29Trusted
Source, 30).
The good news is that heat inactivates these antinutrients. Therefore, cooking
jackfruit seeds by roasting or boiling them will reduce their antinutritional
effects (1).
Jackfruit seeds should not be eaten raw due to their antinutrient content.
Boiling or roasting are two of the most popular preparation methods.
You can boil jackfruit seeds in a large pot of water for 20–30 minutes. Once
the seeds are tender, drain the pot and let them cool and dry.
You can also roast jackfruit seeds by preheating the oven to 400°F (205°C)
and laying them flat on a pan. Bake the seeds for 20 minutes, or until they are
brown and toasted.
You can use jackfruit seeds to add extra flavor and a crunchy texture to
dishes. Due to their large size, they can be consumed in many different ways.
The next time you eat jackfruit, take full advantage of everything it has to offer
by enjoying the seeds along with the delicious, sweet fruit.